Curriculum vitae
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1. Personal data
Full Name
Sara Teresa Neves da Silva
Work Address
ECO-BIO/IICT
Quinta do Marquês (EAN)
2784-505 Oeiras
PORTUGAL
Contacts
Work Phone: 214544680/
Mobile Phone: 938595884
Email: [email protected]
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2. Academic degrees
2008
Degree: Licenciatura (Biology)
Degree granting institution: Universidade Lusófona de Humanidades e Tecnologias
(ULHT)
Final grade: 16
Thesis title: “Characterization of RmNhaE, a New Na+/H+ Antiporter from
Rhodothermus marinus”.
Scientific area: Biological Sciences
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3. Previous activity and current status
October 2007 to June 2008
Undergraduate Student (5th year)
Instituto de Tecnologia Química e Biológica (ITQB) / Instituto de Investigação Científica
Tropical (IICT)
January 2009 Research Grant (BI) student (PTDC/BIA-PRO/67105/2006)
Instituto de Investigação Científica Tropical
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4. Area of scientific activity
Molecular biology, biochemistry of prokaryotes, microbiology.
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5. Present research interest
Domain of specialization
- Bacterial growth under aerobic conditions
- Heterologous expression of membrane proteins
- Preparation, isolation and solubilization of the membrane fraction of bacteria
- Proteomic analysis using denaturing and native gel electrophoresis and second dimension
electrophoresis (2D Tricine-SDS-PAGE)
- Western Blotting
- Enzymatic activity analysis by fluorescence spectroscopy and in-gel activity
- Protein and DNA sequence analysis using bioinformatics tools
- UV-visible absorption spectrometry
- Analysis of catalytic activity using oxygen electrode
- DNA extraction
- PCR, colony PCR, Real-Time PCR
- Cloning in prokaryotes
Current research interests
- Mitochondrial and Prokaryotic Aerobic Respiratory Chains
- Analysis of Supramolecular structures of Prokaryotic Aerobic Respiratory Chains
- Fluorescence Resonance Energy Transfer
- Molecular Biology
- Enzymology, Biochemistry, and Proteomics
Other professional interests/activities
- VIII National Meeting of Biology Students (UCoimbra, 2004)
- IX National Meeting of Biology Students and Workshop “Molecular MacGyver – in
search of the hidden DNA” (UALG, 2005)
- X National Meeting of Biology Students and Workshop “Genetics as a tool for the
study and conservation of biodiversity” (UPorto, 2006)
- X Meeting of the Portuguese Society of Human Genetics (Coimbra, 2006)
- Theoretical and Practical sessions of the course “Introduction to Real-Time PCR: Basic
Aspects and Applications” (FCM/UNL, 2006)
- Organization of the International Conference “Conservation and Animal Welfare”
(ULHT, 2006)
- XI National Meeting of Biology Students and Workshops “Initiation to plant flow
cytometry” and “Polysaccharide fingerprints” (UAveiro, 2007)
- XII National Meeting of Biology Students (UMinho, 2008)
- I Portuguese-Spanish-British Joint Congress of Biophysics (Lisboa, 2008)
- Pathway Analysis in Proteomics (PathProt I) (Oeiras, 2008)
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6. Experience as scientific advisor
- Summer practical course to high school students (Programa Ciência Viva) – “Study of
plants respiratory chain” (IICT Oeiras, 2008)
- Summer practical course to high school students (Programa Ciência Viva) - "Study of the
Supercomplexes of the respiratory chain of Bacillus subtilis" (IICT Oeiras, 2009)
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7. Participation in R&D projects
January 2009 to present
BI Grant Student
Instituto de Investigação Científica Tropical, Oeiras
PTDC/BIA-PRO/67105/2006
"Identification and characterization of the bacterial aerobic respiratory chain supercomplexes
from Escherichia coli and Bacillus subtilis"
June 2008 to January 2009
Instituto de Investigação Científica Tropical, Oeiras
PTDC/BIA-PRO/67105/2006
"Identification and characterization of the bacterial aerobic respiratory chain
supercomplexes from Escherichia coli and Bacillus subtilis"
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10. Communications in scientific meetings
2009
"The aerobic respiratory chain of Escherichia coli harbors a NADH and Succinate Oxidase
Supercomplex" at the Chemistry of Metals in Biological Systems Symposium, in Louvain-laNeuve, Belgium.
2008
- Poster entitled “Evidence for the presence of a Na+/H+ antiporter subunit in
Rhodothermus marinus complex I”, at the European Bioenergetics Conference.
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11. Language skills
Language
English
French
Portuguese
Spanish
Reading
Very Good
Basic
Very Good
Basic
Writing
Very Good
Basic
Very Good
Basic
Conversation
Very Good
Basic
Very Good
Basic