STRESS OXIDATIVE AND RESPIRATORY TREE HISTOMICROGRAPH OF HOLOTHURIA GRISEA EXPOSED TO TIN
PEREIRA, EDGAR ROCHA; PEREIRA,TATIANA MIURA; SANTOS, BRUNA FAÉ; GIORI, ELIANE OLIVEIRA; CASTRO,
RODRIGO ROQUE LESQUEVES E CRUZ, ZILMA MARIA ALMEIDA.
Laboratório de Biomarcadores Ambientais – Universidade Vila Velha, Vila Velha/ES, Brasil
The contamination of the marine environment by heavy metals can induce morphological and physiological changes in
organisms, compromising the reproduction and survival of species. To evaluate tin action, SnCl4, on Catalase (CAT),
Glutathione Reductase (GR), Superoxide Dismutase (SOD), Glutathione-S-transferase (GST) of Holothuria grisea and
alterations on respiratory tree structures. Echinoderms were acclimatized and exposed to the tin concentration 0.3
mg/L; 0.6 mg/L and 0.9 mg/L. The After 96 hours, the animals were sacrificed and organs were used for evaluation of
enzymes and histology of Respiratory tree. Inhibition of SOD was observed in all organs was unlike that of GST alone
showed activity in respiratory tree. Only intestine and muscle showed activity to GR. In presence of 9.0 mg/L, SOD was
inhibited by 84%, 72%, 58% and 46% for muscle, gonads, intestines and respiratory tree, respectively. A similar
response was detected when the organs were assayed for CAT. The histologic analyses show the presence of vacuoles
in the basal pole of the cells and marked dilation of the ducts. Those organisms exposed to 0.6 mg/L were observed
noticeable deterioration of tissues as hydrolysis in some regions of respiratory tree, vacuoles formation in the basal pole
of the cells and marked dilation of the ducts. Finally, when analyzed exposed to 9.0 mg/L, changes were detected in the
above concentrations characterized plus hyalinization the cytoplasm of epithelial cells. Many organisms are used in
biomonitoring of environments contaminated by tin. Holothuria is a restricted mobility organism and well represented in
the marine environment, which makes it interesting for ecotoxicological studies. The respiratory tree makes direct
contact with the chemical dissolved in water, which makes it the object of analysis in ecotoxicology.
Keywords: Element-trace, histology, SOD