Analysis of microbiological quality of "in natura" water in Cascavel city
Cristian Sergio Braga; Simone Gomes Damasceno, Fabiana Andre Falconi.
Universidade Estadual do Oeste do Paraná, Caixa Postal 701, CEP: 85819-110. Cascavel, Paraná, Brasil. E-mail:
[email protected]
keywords: Total coliforms, contamination, E.coli
supply without treatment and admits the presence of total
coliforms in the absence of Escherichia coli or coliform
organisms per 100 mL sample. So, 40% of samples were
Nowadays, water is the main global concern as it is rejected for human consumption, since they were
essential for human life, as well as with regard to its use in contaminated with E.coli.
an inappropriate way and to its maintenance for the
benefit of everyone, in quality and quantity (SÁ, 2005).
The untreated water, mostly, naturally has bacteria due to
their contact with soil and air. Many of these
microorganisms are harmless, but water can also be At last, 40% of the studied samples were contaminated by
microorganisms total coliforms and E.coli, which does not meet the criteria
(SCHULLER, 2004), so, it becomes a vehicle of diarrheal of the Ministry of Health, thus improper for consumption.
diseases of infectious nature. Therefore, an assessment of
water quality is essential, mainly for human consumption
(ISSAC-MARQUEZ et al, 1994).
Thus, this study aimed at determining if "in natura" water
samples, from springs and wells, were in accordance with To the 10 Regional of Health from Paraná State and to the
the current legislation based on total coliforms and municipal government, responsible for the collection of
water samples.
Escherichia coli parameters (BRAZIL, 2004).
The samples were collected in Cascavel, from August 2010
to April 2011, totaling 69 samples. The methodology was
based on the Colilert method to determine total and fecal
coliforms. The sampling collection and methodology are BRASIL. Portaria MS n.º 518/2004 / Ministério da Saúde,
recommended by Standard Methods for the Examination of Secretaria de Vigilância em Saúde, Coordenação Geral de
Water and Wastewater (1995). Results were expressed as Vigilância em Saúde Ambiental – Brasília: Editora do
the absence or presence of total coliforms and E.coli in 100 Ministério da Saúde, 2005. 28 p.
ml of water.
GREENBERG AE, Clesleri LS, Eaton AD, editors.
American Public Health Association (APHA). Standard
methods for the examination of water and wastewater.
The results of total and faecal coliforms count from 20th ed. New York, 1995.
collected samples can be observed on Table 1.
ISSAC-MARQUEZ A.P, Lezama-Davila CM, Ku-Pech
Table 1. Results of total and fecal coliforms analyses RP, Tamay-Segovia P. Calidad sanitaria de los
from water samples collected at wells and springs in suministros de agua para consumo humano en
Campeche. Salud Pública Méx. 36: 655-61, 1994.
Cascavel, Paraná.
nº of
% of
nº of
% of
According to Table 1, from the 69 samples analyzed, 40
(58%) of them were contaminated by total coliforms and 23
(40%) by E. coli. The decree No. 518 of March 25th, 2004,
states that water for human consumption, from individual
sources such as wells, mines, springs and other sources of
SÁ, L.C., Jesus, I.M., Santos, E.C.O.,Vale, E.R., Loureiro,
E.C.B., Sá, E.V. Qualidade microbiológica da água
para consumo humano em duas áreas contempladas
com intervenções de saneamento – Belém do Pará,
Brasil . Revista Epidemiologia e Serviços de Saúde 168,
v. 14, n. 3, jul/set, 2005.
SCHULLER, Dorit. Unidade IX - Microbiologia de água
destinada ao consumo humano. In CASAL, Margarida,
coord. - "Microbiologia e genética molecular
microbiana - manual de laboratório". [S.l.]:
Copissaurio, 2004.

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