Analysis of microbiological quality of "in natura" water in Cascavel city Cristian Sergio Braga; Simone Gomes Damasceno, Fabiana Andre Falconi. Universidade Estadual do Oeste do Paraná, Caixa Postal 701, CEP: 85819-110. Cascavel, Paraná, Brasil. E-mail: [email protected] keywords: Total coliforms, contamination, E.coli supply without treatment and admits the presence of total coliforms in the absence of Escherichia coli or coliform organisms per 100 mL sample. So, 40% of samples were Nowadays, water is the main global concern as it is rejected for human consumption, since they were essential for human life, as well as with regard to its use in contaminated with E.coli. an inappropriate way and to its maintenance for the benefit of everyone, in quality and quantity (SÁ, 2005). CONCLUSION The untreated water, mostly, naturally has bacteria due to their contact with soil and air. Many of these microorganisms are harmless, but water can also be At last, 40% of the studied samples were contaminated by contaminated with pathogenic microorganisms total coliforms and E.coli, which does not meet the criteria (SCHULLER, 2004), so, it becomes a vehicle of diarrheal of the Ministry of Health, thus improper for consumption. diseases of infectious nature. Therefore, an assessment of water quality is essential, mainly for human consumption ACKNOWLEDGMENT (ISSAC-MARQUEZ et al, 1994). Thus, this study aimed at determining if "in natura" water th samples, from springs and wells, were in accordance with To the 10 Regional of Health from Paraná State and to the the current legislation based on total coliforms and municipal government, responsible for the collection of water samples. Escherichia coli parameters (BRAZIL, 2004). The samples were collected in Cascavel, from August 2010 to April 2011, totaling 69 samples. The methodology was based on the Colilert method to determine total and fecal coliforms. The sampling collection and methodology are BRASIL. Portaria MS n.º 518/2004 / Ministério da Saúde, recommended by Standard Methods for the Examination of Secretaria de Vigilância em Saúde, Coordenação Geral de Water and Wastewater (1995). Results were expressed as Vigilância em Saúde Ambiental – Brasília: Editora do the absence or presence of total coliforms and E.coli in 100 Ministério da Saúde, 2005. 28 p. ml of water. GREENBERG AE, Clesleri LS, Eaton AD, editors. American Public Health Association (APHA). Standard RESULTS AND DISCUSSION methods for the examination of water and wastewater. The results of total and faecal coliforms count from 20th ed. New York, 1995. collected samples can be observed on Table 1. ISSAC-MARQUEZ A.P, Lezama-Davila CM, Ku-Pech Table 1. Results of total and fecal coliforms analyses RP, Tamay-Segovia P. Calidad sanitaria de los from water samples collected at wells and springs in suministros de agua para consumo humano en Campeche. Salud Pública Méx. 36: 655-61, 1994. Cascavel, Paraná. INTRODUCTION Absence Total Presence nº of % of nº of % of samples samples samples samples 29 42% 40 58% Coliforms E.coli 46 60% 23 40% According to Table 1, from the 69 samples analyzed, 40 (58%) of them were contaminated by total coliforms and 23 (40%) by E. coli. The decree No. 518 of March 25th, 2004, states that water for human consumption, from individual sources such as wells, mines, springs and other sources of SÁ, L.C., Jesus, I.M., Santos, E.C.O.,Vale, E.R., Loureiro, E.C.B., Sá, E.V. Qualidade microbiológica da água para consumo humano em duas áreas contempladas com intervenções de saneamento – Belém do Pará, Brasil . Revista Epidemiologia e Serviços de Saúde 168, v. 14, n. 3, jul/set, 2005. SCHULLER, Dorit. Unidade IX - Microbiologia de água destinada ao consumo humano. In CASAL, Margarida, coord. - "Microbiologia e genética molecular microbiana - manual de laboratório". [S.l.]: Copissaurio, 2004.