NANOPARTICULAS
MATÁLICAS
QF-435-AULA-8
Prof. Nelson Durán-IQ-UNICAMP
Chem. Eur. J. 2004, 10, 5570 – 5579
Drechsler, Erdogan, and Rotello
Elechiguerra et al. J. Nanobiotechnol. 396) 2005
METALLIC NANOPARTICLES
AS CARRIERS
METALLIC NANOPARTICLES AS CARRIERS
Gimenez et al. J. Biomed. Nanotechnol.
1, 1-7 (2005)
Lewin et al. Nat. Biotechnol.
18, 410 (2000)
HAuCl4 + NaHB4
Ho et al. Anal. Chem. 2004,
76, 7162-7168.
Li et al. nnotechnology 2005, 16, 1912-1917
Lewin et al. Nat Biotechnol. 18, 410 (2000)
http://www.massmedic.com/docs/rotello.pdf
http://www.massmedic.com/docs/rotello.pdf
Mixed Monolayer Protected Gold Clusters (MMPCs)
http://www.massmedic.com/docs/rotello.pdf
Exemplo: Np's de Au
funcionalizadas com anti-câncer para
Leucemia.
• Preparação:
– Redução de Ácido
tetracloroáurico(III)
com Borohidreto de
Sódio.
– Funcionalização após
30s da adição do
agente redutor
6-mercaptopurine
Podsiadlo et al. Langmuir. 24, 568, 2008
PRATA EM PRODUTOS
Pasta de Dente
Que fazem as nanopartículas? Escova de
dentes
Purificador de Ar
Refrigerador
Que fazem as nanopartículas? Sem
comentários
MATERIAS PARA FERIMENTOS
Área superficial grande de partículas
de prata tamanho nano melhora a
efetividade antibacteriana
-melhora os tempos de recuperação
-mata bactéria mais rápido com
nanoparticulas que outras formas
de prata
Como preparar as nanopartícula?
Nanopartículas de prata
Sal do metal + agente redutor
E.C. Constable, Depart. Chemistry, University of Basel, Switzerland
Como preparar as nanopartícula?
Nanopartículas de Au e Pd
Arredondadas, lisas de 18 nm de
partículas de Au por redução
de citrato
Hexagonal de 30 nm de partículas
de Pd preparada por redução de
uma sal de Pd com baixa
concentração de ácido ascórbico
E.C. Constable, Depart. Chemistry, University of Basel, Switzerland
Como preparar as nanopartícula?
Nanopartículas de metais
Conhecidas da antiguidade
Ouro coloidal usada para vidros tingido com vermelho e
púrpura dos tempos medievais até agora
E.C. Constable, Depart. Chemistry, University of Basel, Switzerland
Sintese de nanoparticulas
metalicas
FeCl3 + 6H2O + FeCl2 . 4H2O + NH4OH
85oC
--------------
HAuCl4 + NaBH4 ---------------
[Ag(NH3)2]+ Ac. ascorbico/1 h r.t. --------------
PREPARATION
Chemical Synthesis of silver nanoparticles
Como preparar nanopartículas? Nanocubos de
prata
E.C. Constable, Depart. Chemistry, University of Basel, Switzerland
Como preparar as nanopartícula?
Nanopartículas de óxidos metálicos
E.C. Constable, Depart. Chemistry, University of Basel, Switzerland
Caracterização de nanopartículas de TiO2 por
TEM
E.C. Constable, Depart. Chemistry, University of Basel, Switzerland
Como caracterizar as nanopartículas?
BIOSYNTHESIS OF SILVER
NANOPARTICLES BY
FUNGI
BIOSYNTHESIS OF SILVER NANOPARTICLES BY FUNGI
Fusarium oxysporum
Ahmad et al. Colloids Surf.
B. Biointerfaces 2003, 28,
313-318. Mukherjee et al.
ChemBioChem. 2002, 3,
461-463.
Pune-India
Aspergillus fumigatus
Bhaisa and D´Souza,
Colloids Surf. B. Biointerfaces 2006, 47,
166-164
Munbai-India
BIOSYNTHESIS OF SILVER NANOPARTICLES BY FUNGI
Phoma sp
Chen et al. Lett. Appl. Microbiol.
2003, 37, 105-108
Beijing, China
Phanerochaete chrysoporium Vigneshwaran et al.
Colloids Surf. B. Biointerfaces, 2006, 53, 55-59
Munbai-India
Aspergillus flavus Vigneshwaran et al.
Mat. Lett. 2007, 61, 1413-1418
Munbai-India
Pleurotus sajor-caju
Vigneshwaran et al.
Indian Pat. Appl. 2007:709864
BIOSYNTHESIS OF SILVER NANOPARTICLES BY FUNGI
Durán et al.
J. Biomed. Nanotechnol.
2007, 3, 203-208.
Campinas, SP-Brazil
Fusarium oxysporum
Durán et al. J. Nanobiotechnol.
2005. 3:8, 1-7.
Campinas-SP-Brazil
BIOSYNTHESIS OF SILVER
NANOPARTICLES
BY
BACTERIA AND YEAST
BIOSYNTHESIS OF SILVER NANOPARTICLES BY BACTERIA AND YEAST
Aeromonas sp.
Fu et al. Chin.J.
Chem. Eng.
2006,
14, 114-116.
Xiamen, China
Enterobacter clocae
Shahverdi et al. Process
Biochem. 2007, 42, 919-923
Teheran-Iran
Yeast strain
Kowshik et al.
Nanotechnology
2003,
14, 95-100. Berlin,
Germany
Bacterial Biosynthesis of Cadmium Sulfide Nanocrystals
Sweeney et al.
Chem. Biol. 11, 1553 ()2004)
Cd
N
P
S
Fe
C
O
Sulfeto de Cadmio assistido por Lactobacillus sp.
Jha et al. NANO: Brief Reports and Reviews
Vol. 2, No. 4 (2007) 239–242
Biogenic formation of photoactive arsenic-sulfide
nanotubes by Shewanella sp. strain HN-41.
Lee et al., PNAS, 104, 20410–20415 (2007)
BIOSYNTHESIS OF SILVER
NANOPARTICLES
BY PLANT EXTRACTS
BIOSYNTHESIS OF SILVER NANOPARTICLES BY PLANT EXTRACTS
Neem leaf broth
Shankar et al.J. Colloid
Interf. Sci. 2004, 275,
496-502.
Pune,India
Geranium (Pelargonium graveolens)
Shankar et al. Biotechnol Prog.
2003, 19, 1627-1631
Pune, India
BIOSYNTHESIS OF SILVER NANOPARTICLES BY PLANT EXTRACTS
Cinnamomum camphora leaf
Huang et al. Nanotechnology,
2007, 18, 1-11
Xiamen, China
Alfalfa grass
Gardea-Torresdey, Langmuir 2003,
19, 1357-1361
Texas, USA/Mexico, Mexico
Aloe vera plant extract
Chandran et al., Biotechnol Prog. 2006, 22, 577-583.
Pune, India
Emblica Officinalis
Ankamwar et al., J. Nanosc. Nanotechnol. 5, 16651671.
Pune, India
MECHANSTIC ASPECTS OF
BIOSYNTHESIS OF SILVER
NANOPARTICLES
MECHANSTIC ASPECTS OF BIOSYNTHESIS OF SILVER NANOPARTICLES
The
silver-binding
peptides
from
Pseudomonas stutzeriAG259 cells were
obtained by using a combinatorial
approach to identify these peptides from
a phage display library of random
peptides. The interaction of peptide with
the metal clusters provides a chemically
reducing environment around the cluster,
thereby allowing further accelerated
reduction of silver ions at the interface
between peptide and metal.
Naik et al. Nature Mater. 2002,
1, 169-172. Ohio, USA
MECHANSTIC ASPECTS OF BIOSYNTHESIS OF SILVER NANOPARTICLES
Si and Mandal, Chem Eur. J. 2007, 13, 3160-3168.
Kolkata, India
Similar results with tryptophan and gold
Selvakannan et al. J. Colloids Interf. Sci. 2004, 269, 97-102
Bhattacharjee et al. J. Nanosci. Nanotechnol. 2005, 5, 1141-1147.
MECHANSTIC ASPECTS OF BIOSYNTHESIS OF SILVER NANOPARTICLES
Selvakannan et al. Langmuir, 2004, 20, 7825-7836.
Pune, India
Specific for Au
Stacik et al. J. Mater Chem. 2005, 15, 749-753
MECHANSTIC ASPECTS OF BIOSYNTHESIS OF SILVER NANOPARTICLES
Fusarium oxysporum
Durán et al. J. Nanobiotechnol. 2005. 3:8, 1-7.
Campinas-SP-Brazil
Durán et al.
J. Biomed. Nanotechnol. 2007, 3, 203-208.
Campinas, SP-Brazil
Fusarium moniliforme was negative in quinone production
SILVER NANOPARTICLES
AGAINST PATHOGENS
SILVER NANOPARTICLES AS ANTIBIOTIC CARRIERS
P. Li, J. Li, C. Wu, Q. Wu,J. Li. Synergistic antibacterial effects
of β-lactam antibiotic combined with silver nanoparticles.
Nanotechnology 16, 1912–1917 (2005)
Without any antibiotics
5 ug/mL of silver nanoparticles
150 ug/mL of amoxicillin
150 ug/mL of amoxicillin + 5 ug/mL of
silver nanoparticles
SILVER NANOPARTICLES AS ANTIBIOTIC CARRIERS
E. coli 5 x 106 cfu bac., a) 5 ug mL-1 silver nanoparticles b) 0.150 ug mL-1 antibiotic,
c) 0.150 ug mL-1 antibiotic + a), d) b) + 10 ug mL-1 silver nanoparticles
SILVER NANOPARTICLES AS ANTIBIOTIC CARRIERS
AMOXICILLIN MECHANISM
Li et al., Nanotechnology 16, 1912 (2005)
SILVER NANOPARTICLES AS ANTIBIOTIC CARRIERS
CLINDAMYCIN
SILVER NANOPARTICLES AS ANTIBIOTIC CARRIERS
CLIN
CLIN
CLIN
CLIN
CLIN
CLIN
CLIN
CLIN
Chemical and fungal synthesis
Brocchi et al. J. Nanosci. Nanotechnol., submitted (2008)
SILVER NANOPARTICLES AS ANTIBIOTIC CARRIERS
MINIMUM INHIBITION CONCENTRATION (MIC)
Durán et al., Crit. Rev. Microbiol. Submitted (2008)
VANCOMICINA EM NANOPARTICULAS PARA AUMENTAR
SUA ATIVIDADE ANTIMICROBIANA
Gu et al. Nano Lett. 3, 1261-1263 (2003)
SINTESE DE NANOPARTICULAS DE OURO COM
VANCOMICINA
SILVER NANOPARTICLES
AND NEGLECTED DISEASES
SILVER NANOPARTICLES AND NEGLECTED DISEASES
Goal
Cutaneous leishmaniasis
SILVER NANOPARTICLES AND NEGLECTED DISEASES
CLIN
CLIN
CLIN
CLIN
CLIN
CLIN
CLIN
CLIN
Clindamycin associated to silver nanoparticles could be effective in these
neglected diseases
SILVER NANOPARTICLES
AND ITS TOXICITY
Mitochondrial function through MTS
C18-4 spermatogonial stem cells
Ag nanoparticles 15 nm
Membrane leakage though lactate dihydrogenase ( LDH)
Silver nanoparticles (15 nm) reduced mitochondrial function drastically and
increased membrane leakage. Our data show that the C18-4 cells are more
sensitive than the BRL 3A cells in that respect (Braydich-Stolle et al. ToxSci
Advance Access published July 13, 2005).
Pal et al., 73, 712 (2007)
Pal et al., 73, 712 (2007)
SILVER NANOPARTICLES AND NEGLECTED DISEASES
SKIN PERMEATION IN VITRO
20-50 nm
Human abdominal skin
The nanoparticles did not reach the
receptor compartment
SILVER NANOPARTICLES AND NEGLECTED DISEASES
ANTIBACTERIAL EFFECT OF SILVER NANOPARTICLES
Silver nanoparticles from Aspergillus niger
Gade et al., Appl. Microbiol. Biotechnol., submitted (2008)
(Amravati-India/Campinas SP-Brazil)
PREPARATION
Durán et al. 2005,2007,2008
Biosynthesis of silver nanoparticles
Fusarium oxysporum was grown by 7 days
The biomass was filtrated and resuspended in sterile water
The biomass was filtrated and AgNO3 (10 mM) was added
in the fungal liquid
Absorptions were measured in UV-Vis
Durán et al. J. Nanobiotechnol. 3, 1-7 (2005).
MATERIAIS E MÉTODOS
Coleção de 20 linhagens de Fusarium
Culturas de
Fusarium spp
MEV - nanopartículas
Espectro UV-vis
0,017/10-3 M de AgNO3
12 Horas
Solução coloidal
de reação Caldo fungal
Cultura em
meio
líquido (YM)
72 Horas
Biomassa em
água destl.
Para seleção da linhagem com melhor eficiência, levamos em
consideração os dois métodos de biossíntese, tanto usando a
biomassa quanto usando o líquido fungal
A
Métodos
No
caldo,
foram
introduzindo,017/10-3
M de AgNO3
Nanopartículas de
prata.
B
Após ser separada a
biomassa da água
destilada. Este peso
úmido
foi
adicionada em uma
solução de 0,017/10-3
M de AgNO3 .
Souza et al. 58º SBPC, Florianopolis (2006)
Utilizando as
nanopartículas
da melhor
linhagem
Nanopartículas
Impregnação em
tecido têxtil para
Algodão 100%
Poliéster
atividade biocida
Teste oligodinâmica
Staphylococcus aureus
Klebisiela pneumoniae
Durán et al. Patente Braz. PIBr 0605681-4 (2006)
PRÉ-ENSAIO E RECUPERAÇÃO DAS NANOPARTÍCULAS EM
EFLUENTES
A água de lavagem do tecido têxtil impregnado com
nanopartículas de prata, foi submetida ao tratamento C.
violaceum.
100mL/100mL
100mL/60mL
100mL/20mL
48 Horas
sob
agitação
Aeração (0,5
L/mim)
Análise
espectro UV-vis
Ausência de
ar
Durán et al. J. Biomed. Nanotechnol. 3, 203-208 (2007).
RESULTADOS
•Espectro
UV-vis
de
linhagens
de
produtoras de nanopartículas de prata.
Souza et al. 58º SBPC, Florianopolis, S.C. (2006)
Fusarium
Silver nanoparticles
Size: 1,6 nm
(biosynthesis)
Durán et al. J. Nanobiotechnol. 2005
Silver nanoparticles
Size: 175 nm
(Chemical synthesis)
a
Amostras centrifugadas
Espectro UV-VÍS
b
Impregnação das nanopartículas em a) algodão e
b) polyester
MEV do extrato líquido
1) Algodão 100% e 2) polyester 100% 3) Cultura de Staphylococus
aureus
e
4)
Teste
com
tecido
algodão
impregnado
com
nanopartículas de prata frente à cultura bacteriana e 5) teste do
tecido polyester impregnado com nanopartículas de prata frente à
cultura bacteriana
1
4
3
2
5
ATIVIDADE BIOCIDA DO TECIDO IMPREGNADO
(ALGODÃO) COM NANOPARTÍCULAS DE PRATA
frente a bactéria S. aureus
MICROSCOPIA ELETRÔNICA DE VARREDURA DO TRATAMENTO
DE ALGODÃO COM NANOPARTICULAS DE PRATA
Tecido Controle (sem nanopartículas de prata)
Tecido com
nanopartículas de
prata
Durán et al. J. Biomed. Nanotechnol. 3, 203-208 (2007).
ATIVIDADE
BIOCIDA
DO
TECIDO
IMPREGNADO
(POLIÉSTER) COM NANOPARTÍCULAS DE PRATA frente a
bactéria S. aureus
M.O das amostras não impregnadas
M.O
do
impregnado
nps.
Poliéster
com
POSSÍVEL RECUPERAÇÃO
•Os tecidos impregnados com nanopartículas, após
sucessivas lavagens, perdem gradativamente sua
atividade biocida, uma vez que as nanopartículas
são liberadas durante este processo.
•Para evitar o descarte de nanopartículas no
ambiente, foram realizados ensaios com diferentes
concentrações
de
inóculo
da
bactéria,
Chromobacterium violaceum.
Espectros A e B, correspondentes às amostras controle (A, sem
diluição e B, diluição de 10 vezes). (C, D e E submetidas à
aeração). (F, G e H submetidas à ausência de ar) todas tratadas
com Chromobacterium violaceum.
EDS: Espectro de nanopartículas de prata em bactéria
Chromobacterium violaceum.
•Todas
CONCLUSÃO
as 20 linhagens de Fusarium utilizadas neste trabalho
apresentaram capacidade de produção de nanopartículas de prata,
entretanto, foram as três mais eficientes separadas (dados apresentados
no Relatório Parcial). Destas, foi selecionada a linhagem 07SD.
•Avaliaram-se dois métodos de biossíntese: um empregando diretamente
a biomassa do fungo e outro apenas o líquido fungal.
Este último
mostrou melhores resultados. Logo, deu-se continuidade ao trabalho
empregando este método.
•Os tecidos impregnados com as nanopartículas de prata apresentaram
atividade biocida frente a Staphylococcus aureus e Klebsiella pneunomiae.
•A
possibilidade de recuperação das nanopartículas de prata após
lavagens sucessivas dos tecidos utilizando Chromobacterium violaceum
foi comprovada, podendo ser empregada no reaproveitamento contínuo
dessas nanopartículas (ainda em teste)
Durán et al. J. Biomed. Nanotechnol. 3, 203-208 (2007).
Jun Tian, Kenneth K. Y. Wong, Chi-Ming Ho, Chun-Nam Lok,
Wing-Yiu Yu,] Chi-Ming Che, Jen-Fu Chiu, and Paul K. H. Tam.
Topical delivery of silver nanoparticles promotes wound
healing.ChemMedChem.(2007)2,129-136.
• Preparation of the dressings:
• Nanosilver-coated dressing: Initial experiments were carried
out by using silver nanoparticle grafted dressing (silver
content: 2.75 mgg1, Anson Nanotechnology Group Co., Ltd.
Hong Kong). A piece of silver nanoparticle-coated dressing
(4H3 cm2) weighed 0.1737 g. This corresponds to 0.4777 mg of
silver nanoparticles on each piece. Transmission electron
microscopy revealed that silver nanoparticles were spherical,
with diameters of 149.8 nm based on 750 particle
measurements on five sites of interest. Subsequently
experiments were performed with silver nanoparticles (1 mm)
synthesized by borohydride reduction of AgNO3 in the
presence of citrate as a stabilizing agent, as previously
described.[45] Similar efficacy with silver nanoparticle dressing
was confirmed (data not shown).
SSD
NT
ND
Silver Nano
Silver sulfadiazine
E:epidermid
HF: hair follicles
CULTURA DE MICRORGANISMOS NO PANO DA FERIDA NOS DIAS 1, 3, 5, 7 E 10 NOS ANIMAIS
TRATADOS COM ND (GRIS), SSD (BRANCO E NÃO TRATADO (GRIS ESCURO)
silver nanoparticles can modulate local and systemic
inflammatory response following burn injury by cytokine
modulation.
IL-6
 ND
▄ No treatment
▲ SSD
IL-6 aumenta inflamação
TGF-beta auenta inflamação
Il-10regula citokinas
VGEF (vascular endotelial
growth factor)
promove cicatrização
IFN-gama aumenta colageno
TGF-beta
VGEF
IL-10
IFN-gama
PATENTES EM COSMÉTICOS
•
•
•
•
•
•
•
•
•
•
Facial mask comprising a nanometer metal coating. Ko, Chuan Tao. (Taiwan). U.S. Pat. Appl. Publ.
(2007), 5pp. CODEN: USXXCO US 2007125383
Silver-containing an antimicrobial body care product. Hanke, Bernhard. (Procter and Gamble Company,
USA). PCT Int. Appl. (2000), 22 pp. CODEN: PIXXD2 WO 2000078281
Antimicrobial gel formulation containing silver nanoparticles for treating female genital infections, its
preparation method and uses. Chen, Hu. (Shenzhen Agt Pharm. Co., Ltd., Peop. Rep. China). Faming
Zhuanli Shenqing Gongkai Shuomingshu (2005), 16pp. CODEN: CNXXEV CN 1672689 A
Manufacture of cosmetics containing silver nanoparticles and Carbopol. Liu, Baning; Ma, Xiaoling; Liu,
Xi. (Peop. Rep. China). Faming Zhuanli Shenqing Gongkai Shuomingshu (2004), No pp. given.
CODEN: CNXXEV CN 1539403 A
Formulation of UV absorbers by incorporation in solid lipid nanoparticles. Herzog, Bernd. (Ciba
Specialty Chemicals Corporation, USA). U.S. Pat. Appl. Publ. (2003), 16 pp. CODEN: USXXCO US
2003235540 A1
Pharmaceutical compositions for treating allergic dermatitis comprising nano-gold solution. Han, Sang
Pil; Yoo, Eui Jae; Choi, Paeck Hi; Choi, Young Deuk. (Hanssem Health Co., Ltd., S. Korea). Repub.
Korean Kongkae Taeho Kongbo (2006), No pp. given. CODEN: KRXXA7 KR 2006134261
Hair preparations containing silver nanoparticle colloids for the treatment of bacteria-caused hair
depilation. Han, Sang Pil; Yoo, Eui Jae; Choi, Paeck Hi; Choi, Young Deuk. (Hanssem Health Co., Ltd.,
S. Korea). Repub. Korean Kongkae Taeho Kongbo (2006), No pp. given. CODEN: KRXXA7 KR
2006110600
Vaginal adhesive preparation containing silver nanoparticles and its preparation method. Gu, Maojian;
Ren, Fuzheng; Liang, Wei; Jiang, Zongrun. (Shanghai Jnj Modern Pharmaceutical Technologies Inc.,
Peop. Rep. China). Faming Zhuanli Shenqing Gongkai Shuomingshu (2006), 14pp. CODEN: CNXXEV
CN 1857310
Anti-microbial condom containing silver nanoparticles, capable of preventing various adult diseases and
pregnancy. Yang, Won Dong. (S. Korea). Repub. Korean Kongkae Taeho Kongbo (2005), No pp.
given. CODEN: KRXXA7 KR 2005114182
Antiaging cosmetics containing gold nanoparticles and silk fibroin for maintaining antioxidant activity.
Hur, Won; Lim, Kun Bin; Ahn, Hyo Soon; Shin, Hwa Sook. (Fineco. Ltd., S. Korea). Repub. Korean
Kongkae Taeho Kongbo (2006), No pp. given. CODEN: KRXXA7 KR 2006108789
SINTESE DE NANOPARTICULAS
DE OURO ASSOCIADA A IgG
HAuCl4 + NaBH4
IgG de soro humano
BACTERIA
BACTERIA
Imã
SEPARAÇÃO
ANALISE
MLDI Analysis: (H. Steen and M. Mann. “The abc’s (and xyz’s) of peptide
sequencing”. Nat. Rev. Mol. Cell Biol. 5, 699-711 (2003)).
Staphylococcus saprophyticus were collected from patients at the Hospital,
Streptococcus pyogenes JRS 75 and JRS 4 were from collections S. pyogenes
JRS 75 was obtained by mutating M protein from the strain of S. pyogenes
JRS 4.29
The lowest cell concentration that was detected for both Staphylococcus
saprophyticus and Staphylococcus aureus in aqueous sample solutions (0.5
mL) was 3 x 10(5) cfu/mL, while the detectable cell concentration for S.
saprophyticus in a urine sample was 3 x 10(7) cfu/mL
Absorption and desorption of chemotherapeutic drugs
from a magnectically targeted carrier (MTC). Rudger et al.
J. Control. Releae 74, 335-340 (2001).
Doxorubicin follows the Langmuir theory for
milling, particles are resuspended and dispersed,
adsorption
to
MTCs.
The
Langmuir
theory
holds that the solute adsorbs to sites on the carbon
surface in a discrete one to one correspondence.
ACTIVATED CARBON
MAGNETICALLY TARGETED CARRIER (MTC)
ALVOS NAO ESPECIFICOS
------------------------------------------------------------------------------------------------------
Rudger et al. J. Control. Releae 74, 335-340 (2001)
Treatment of a hepatocellular carcinoma by
MTCs in clinical human trials
As shown in the left panel following the treatment of a HCC tumor with MCT-DOX the hepatic arteries
Remain patent, demonstrating particles toletaribility as evidenced by the lack of trombosis and/or
embolization of the arteries. Localization and retention of the particles in the target tumor are showed
in the the righ papel by magnetic resonant imagins (MIR) (the particles were in the local after 28 days)
PATENTES EM COSMÉTICOS
•
•
•
•
•
•
•
•
•
•
Facial mask comprising a nanometer metal coating. Ko, Chuan Tao. (Taiwan). U.S. Pat. Appl. Publ.
(2007), 5pp. CODEN: USXXCO US 2007125383
Silver-containing an antimicrobial body care product. Hanke, Bernhard. (Procter and Gamble Company,
USA). PCT Int. Appl. (2000), 22 pp. CODEN: PIXXD2 WO 2000078281
Antimicrobial gel formulation containing silver nanoparticles for treating female genital infections, its
preparation method and uses. Chen, Hu. (Shenzhen Agt Pharm. Co., Ltd., Peop. Rep. China). Faming
Zhuanli Shenqing Gongkai Shuomingshu (2005), 16pp. CODEN: CNXXEV CN 1672689 A
Manufacture of cosmetics containing silver nanoparticles and Carbopol. Liu, Baning; Ma, Xiaoling; Liu,
Xi. (Peop. Rep. China). Faming Zhuanli Shenqing Gongkai Shuomingshu (2004), No pp. given.
CODEN: CNXXEV CN 1539403 A
Formulation of UV absorbers by incorporation in solid lipid nanoparticles. Herzog, Bernd. (Ciba
Specialty Chemicals Corporation, USA). U.S. Pat. Appl. Publ. (2003), 16 pp. CODEN: USXXCO US
2003235540 A1
Pharmaceutical compositions for treating allergic dermatitis comprising nano-gold solution. Han, Sang
Pil; Yoo, Eui Jae; Choi, Paeck Hi; Choi, Young Deuk. (Hanssem Health Co., Ltd., S. Korea). Repub.
Korean Kongkae Taeho Kongbo (2006), No pp. given. CODEN: KRXXA7 KR 2006134261
Hair preparations containing silver nanoparticle colloids for the treatment of bacteria-caused hair
depilation. Han, Sang Pil; Yoo, Eui Jae; Choi, Paeck Hi; Choi, Young Deuk. (Hanssem Health Co., Ltd.,
S. Korea). Repub. Korean Kongkae Taeho Kongbo (2006), No pp. given. CODEN: KRXXA7 KR
2006110600
Vaginal adhesive preparation containing silver nanoparticles and its preparation method. Gu, Maojian;
Ren, Fuzheng; Liang, Wei; Jiang, Zongrun. (Shanghai Jnj Modern Pharmaceutical Technologies Inc.,
Peop. Rep. China). Faming Zhuanli Shenqing Gongkai Shuomingshu (2006), 14pp. CODEN: CNXXEV
CN 1857310
Anti-microbial condom containing silver nanoparticles, capable of preventing various adult diseases and
pregnancy. Yang, Won Dong. (S. Korea). Repub. Korean Kongkae Taeho Kongbo (2005), No pp.
given. CODEN: KRXXA7 KR 2005114182
Antiaging cosmetics containing gold nanoparticles and silk fibroin for maintaining antioxidant activity.
Hur, Won; Lim, Kun Bin; Ahn, Hyo Soon; Shin, Hwa Sook. (Fineco. Ltd., S. Korea). Repub. Korean
Kongkae Taeho Kongbo (2006), No pp. given. CODEN: KRXXA7 KR 2006108789
AGRADECIMENTOS
REDE NANOTUB0S
DE CARBONO
CNPq/MCT
CNPq/MCT