USE OF DIGITAL IMAGES TO DETECTION OF MELAMINE BASED ON
INTERACTION BETWEEN MELAMINE AND GOLD NANOPARTICLES
Dayana Borges Bittar, Tiago Augusto Catelani, Karina Nigoghossian, Hernane da Silva
Barud, Sidney José Lima Ribeiro, Leonardo Pezza and Helena Redigolo Pezza
Instituto de Química, Universidade Estadual Paulista “Julio de Mesquita Filho”, UNESP, R. Prof.
Francisco Degni 55, P.O. Box 355, 14800-900, Araraquara, SP, Brazil.
Adulteration of dairy products, especially involving milk products contaminated with
melamine (1,3,5-triazine-2,4,6-triamine) have created a widespread food safety
concern. Due to the serious health concerns associated with melamine consumption
rapid, inexpensive, and sensitive methods to detect melamine are essential. The
speed and portability are important features required by new analytical methodologies
concerning the detection of contaminants / adulterants in food. In this context, this
study aims to test the applicability of a software on line free (GIMP 2.8) in the
treatment of images obtained in a touch plate, from a mobile device, and correlate
these data with the concentration of contaminant melamine. Colorimetric gold
nanoparticles sensor was developed for determination of melamine. This technique is
the indirect colorimetry, which is measured in the intensity of light reflected by the
solution, addressing the RGB color model. This is a low cost method, which requires
microliters of reagents, an important factor when working with expensive materials or
high-grade toxicity satisfying in this way, some principles of Green Chemistry. The
quantification of analyte was performed work from the calibration curve method (Abs =
- 0.12389 - 0.46697.Cmelamine; R = - 0.987), which was constructed by a linear track
Working from 0.3 to 0.7 mg L-1 having an RSD < 8% LOD =0.0729 and LOQ = 0.2431.
Further tests will be conducted with real food samples contaminated with melamine.
Keywords: Melamine, Gold Nanoparticles, Digital Image, colorimetry.