Mica.
TU 202
Maxlmum resldues IImit of ethylenebisdithiocarbamates In papaya
2
2
Nadia Regina Rodrigues\ David Martins , Oswaldo Yamanishl , Alfredo J05.6 Barreto Luiz - Luiz" Rosângela Abakerli 3 , Elisabeth Fa/, Valdique Medina2, Tarcilo
.
,
Galvão
12UNICAMP, CAMPINAS, Brazil
3Private consultant, Brazil
This work demonstrates tIlat the endogenous levei of CS2 in papava fruits is bigger than the dithiocarbamate MRL established in EU and, therefore, its residues
determination by measuring the evolved CS2 is prone of false positive results. Papaya trees treated and untreated with EBDC fungicide supplied samples in which
the CS2 leveis were estimated by GC/FPD. From the distribution of the empirical accumulated probability functions Df untreated and EBDC treated samples a cut off
value was calculated that allowed to classify the results in with or without residues. lhe quality of this classification function was evaluated through a matrix of error
and the 'K-HAr statistics. The value that best discriminates between endogenous CS2 and true EBDC residues is 0.36 mg kg-1; bigger values would indicate the
use of EBDC.
TU 203
Trace elements and organlc mercury (Org-Hg) In tlssues offranciscana dolphln (Pontoporla blainvillei~ from Two Brazlilan coast areas
3
4
Tércia Seixas\ Helena Kehrig\ Gilberto Fillmann2, Ana Paula Di Beneditt0 , Cristina Souza3, Eduardo Secchi ,Isabel Moreira , Olaf Malm '
'UFRJ/IBCCF, RIO DE JANEIRO, Brazil
2FURG, RIO GRANDE, Brazll
3UENF, CAMPOS DOS GOYTACAZES, Brazil
4PUC-Rio, RIO DE JANEIRO, Brazll
Despite animais collected at both areas presented similar body length, Individuais from southeastem coast were older than the ones from the SOUth, because they
belong to distinct populations. No signlficant gender differences were observed in concentration of elements. Animais trom tIle south presented the highest renal
and hepatic Hg, Se, Cd, As, Cu and Pb concentrations. Org-Hg in the South dolphins were higher than in the Southeast. Uver shOwed the highest Hg, whereas
highest Cd was found in kidney. As and Pb in both organs were similar. Hepatic Hg, Cd, Se, As and Pb increase with body length (L), whereas hepatic Cu
decreases with L. Inlerelement relationships in organs were also investlgated. Hepatlc Hg and Se presented a significant correlation, presenting the Se:Hg ratios
ciosa to 4 in both group areas. Differences found among the elements tlssues trom both areas were related to tIle preferred prey, bioavailability in the marine
environment, and a1so th9 environment conditions.
TU 204
Mercury specles of and selenlum In dolphln IIver
1
1
Helena Kehrig , Tércia Seixas" Ana Paula Di Beneditto2, Cristina Souza2, Olaf Malm
'UFRJ/lnstituto de BiDffsica Carlos Chag, RIO DE JANEIRO, Brazil
2UENF, CAMPOS DOS GOYTACAZES, Brazil
High hepatic Hg and Se are probably related to the role played by liver in terms Df pollutant bio-transformation. In order to assess the importance of liver in
regulation Df Hg accumulation, 4 types of Hg species Onorganic Hg (Inorg Hg), methylmercury (MeHg), other organic Hg (Org Hg) and HgSe) and (Se) were
analyzed in liver (N=12) Df a doIphin species from Brazilian coast. Hg and Se (in a dry wt.) ranged from 0.84 to 137.92 mg HglKg and 1.79 to 69.98 mg SelKg.
MeHg was the smallest fraction of Hg presented, with an average of 19 % (2 % to 43 %). The highest tractlon of Hg was as HgSe, corresponding to 35 % o % to
89 %). lhe highest % MeHg and the lowest % HgSe occurred in Hvers that presenteei Hg below 1.0 mgIKg. The highest HgSe (> 44%) occurred in livers that
presenteei Hg above 5.5 mgIKg. Org Hg made up 24 % anel lnorg Hg 22 % of Hg. The low % MeHg anel the high % HgSe Df ali animais were related to different
capacities ar strategles Df detoxificatiOn Df MeHg in thls organ.
r
TUlO5
Subchronlc alumlnum etrects on osmo-lonic and hematologlcal parameters of a neotropical flsh
MaJina Mori Pires de Camargo" Mansa Fernandes" Cláudia Martine:!
1Universidade Federal de São carlos, MARluA, Brazil
2Universidade Estadual de londrina, LONDRINA, Brazll
The toxicity Df aluminum is well known to fish and however there is a lack of studies about the effects of AI in tropical freshwater fish. lhus the goaJ of this study was
to assay tIle effects Df tlis metal in the fish Proctilodus Ilneatus. lherefore, juveniles Df P. lineatus were exposed for 24 and 96 h and 15 days to 0,1 ppm Df
dissolved AI or only to clean water (control). After that, fish were anesthetized and blood was used fOr the following analysis: hemoglobin (Hb), hematocrit (Hct), red
b\ood cells count (RBC), plasmatic concentrations of glucose, sodium (Na+), chloride (CI-) anel osmolarity. Rsh exposed to AI showed a significant increase in the
Hb, Hct anel RBC when compared to the respective controls in ali exposure periods, and they also showed hyperglycemia after 24 and 96 h. Animais exposed to AI
for 24 h showed a translent decrease Na+ anel 05molarlty In comparision to controls. Nevertheless these parameters plus blood CI· increased significantly in relation
to controls after 15 d of exposure. These results indicate that AI caused osmo-ionic anel hematological disturbances to tIlis neotropical flsh specles, which can Impair
lhe survival of this fish.
TU 206
Hlstopathologlcal altaratlons In the gUls of Clarias garleplnus as an Indicator of exposure to environmental toxlcants
2
Marcelle Marchand\ Ina Pleterse\ lrene Bamhoom , Riana Bomman2
'University Df Johannesburg, JOHANNESBURG, South Africa
~ept of Urology, University of Pretoria, PRETORIA, South AfrIca
The RieMei Nature Reserve in Pretoria, South Africa serves to provide the area with water and protect the natural environment. For this reason the catchment area
needs to be conserved and water needs to be accumulateel anel distributed. Within the reserve are two dams, the Marais - anel Rietvlei Dam, separated by a natural
wetland. Atthough the dams are known to have elevated leveis of toxicants they provlde a habitat for tIle sharptooth catfish, ClaJias gariepinus. The gills of C.
gariepinus were qualitatlvely and quantitatively assessed to identify histopathological alterations as a result of exposure to environmental toxicants. Water samples
were anatyzed for selected metais and potential endocrlne disrupting chemlcals. Chemical toxicants anel metais in the water Df both dams were present at leveis that
could be toxic or endocrlne disruptive. Examination Df gill tissue from 162 specimens reveaied marked histopathological alterations. The alterations included
aneurysms (telangiectasia), oedema of secondary lamenae, anel hyperplasia of lamellar epithelium.
TU 207
PerslBtent organochlorlne pollutants and stable Isotopes In feathers of blrds during fali and sprlng migration In a subtropical region of Western Mexlco
Miguel Mora
U.S. Geological Survey, COLLEGE STATION, TEXAS, United States of America
The objective of this study was to assess the impact Df POPs on birds during the fali and spring migration in westem Michoacán, Mexico. Passerine insectivorous
birds were collected during fali and spring, 2001-2002. The carcasses were analyzed for organochlorine compourds, Tall feathers were analyzed for stable
isotopes of C, N, and H. Organochlorine compounds detected in more than 50% of the saJ1llles were: oxychlordane, p,p'·DDE, p,p'-DDT and Total PCBs. DOE was
the CC detected at the highest concentration. There were no signlficaot differences in concentrations of OCs among species, between seasons, or between resident
and migrant birds. Concentrations of DOE were approxlmately two-fold higher in birds during spring than in fali, however; concentrations were not slgnificantly
different. Two resident birds collected in fali and spring had DOE resiclues of 12,103 and 10,677 nglg wet weight, respectively. There were no signlficant differences
in d13C anel d15N values among species, between seasons or in migrant and resident birds. However, d2H values were different between species and helped
differentiate migrant trom resident birds. Our results suggest that during fali and spring there is not a significant buildup of persistent OCs in migrant or resident
passerine birds in nor1hWest Michoacán, Mexico.
TU 208
Hematological changes in freshwater fish Colossoma macropomum during cadmlum exposure and after subsequent depuration.
2
Raquel Salazar\ Raquel Salaza.r', Alida León\ Mairin Lemus1 , Luisa Centen0 , Kung Chung'
-