rd 23 Congress of the International Union for Biochemistry and Molecular Biology th 44 Annual Meeting of the Brazilian Society for Biochemistry and Molecular Biology th th Foz do Iguaçu, PR, Brazil, August 24 to 28 , 2015 PROTEIN PROFILE OF Fusarium oxysporum AND Phanerochaete chrysosporium DURING THE LAPACHOL BIOTRANSFORMATION PROCESS. Coitinho, L.B.1; Siqueira A.C.R1; Emery, F.S.1; Cabral, H.1 1 Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo. Biotransformation is an important process in enzyme related chemical transformations. Fungi can be considered as a promising source of new compounds, mainly for chiral reactions. Chemo-, regio-, and stereoselective processes are very important in the synthesis of many chemical and pharmaceutical intermediates. Lapachol is a naphthoquinone that presents many interesting biological activities, which have potential uses in the pharmaceutical industry. The aims of this project are to analyze the protein profile of Fusarium oxysporum and Phanerochaete chrysosporium during the lapachol biotransformation process. The microorganism was cultivated in 250 mL Erlenmeyer flasks containing 50 mL of soy-glucose medium and incubated on a rotary shaker at 120 rpm at 30°C. After 24 hours, the mycelial mass was transferred to a Czapeck medium containing lapachol solution in DMSO (final concentration of 50 µg/mL) and fermentation was continued for 24 and 48 hours. The secretome and proteome were obtained through precipitation followed by SDS-Page 12%. In addition, the proteolytic and lipase activities were determined using fluorescenceresonance energy transfer (FRET) substrate and 4-nitrophenyl palmitate, respectively. The obtained results suggest that lapachol modifies the profile of the proteins production compared to the control. Both proteomes of P. chrysosporium and F. oxysporum presented protease activity, although both controls showed a higher production in comparison to the fermentation with lapachol. Moreover, both secretome during the biotransformation process did not show any protease activity. The proteome and secretome of P. chrysosporium in the presence of lapachol did not show lipase activity whereas F. oxysporum showed lipase activity. According to the results, the fungi modify their protein profile when exposed to lapachol during the biotransformation process. Acknowledgements: Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP). Processo Nº 2014/01497-9 e 2012/24703-8 Key Words: Biotransformation; Lapachol; Proteins. Brazilian Society for Biochemistry and Molecular Biology (SBBq)