XII Congresso Brasileiro de Ecotoxicologia 25 a 28 de setembro de 2012 Porto de Galinhas – PE TOXICITY ASSESSMENT OF THE PHARMACEUTICAL COMPOUND TRICLOSAN ON FRESHWATER INVERTEBRATES WITH SPIKED SEDIMENTS Fabio H. Pusceddu1; Fernando S. Cortez2; Aldo R. Santos2; Camilo D.Seabra Pereira3; Augusto Cesar3; Maria B. Bohrer-Morel1 [email protected] (Instituto de Pesquisas Energéticas e Nucleares – Universidade de São Paulo, São Paulo, São Paulo) 2 [email protected] (Instituto de Pesquisas Energéticas e Nucleares – Universidade de São Paulo, São Paulo, São Paulo) 2 [email protected] (Universidade Santa Cecília, Santos, São Paulo) 3 [email protected] (Universidade Federal de São Paulo, Santos, São Paulo) 3 [email protected] (Universidade Federal de São Paulo, Santos, São Paulo) 1 [email protected] (Instituto de Pesquisas Energéticas e Nucleares – Universidade de São Paulo, São Paulo, São Paulo) 1 The increase of Pharmaceutical and Personal Care Products (PPCP) occurrence in the aquatic environment cause adverse effects on the human health and aquatic communities. Triclosan (TCS) is a pharmaceutical compound widely used because of its bactericidal mechanism of action, found in at least 932 products such as shampoos, toilet soaps, deodorants, lotions, toothpaste, detergents and socks, among others. TCS by photodegradation results into dichlorodibenzo-p-dioxin, and has great discussion on environmental agencies about the use or restriction of this product. The aim of this work is to assess biological effects of TCS to Chironomus xanthus and Ceriodaphnia dubia exposed in spiked sediments. The spiked-sediment in the present study was carried through the "slurry spiking" technique. The evaluations were performed by acute toxicity assays with Chironomus xanthus and chronic toxicity assays and teratogens effects with Ceriodaphnia dubia. The CE50/96h obtained on acute toxicity assays with C. xanthus was 45.26 mg.kg-1 and NOEC and LOEC to C. dubia were 5.78 e 6.94 mg.kg-1, respectively. The neonates produced during the experiment have not showed malformations. Using equilibrium partitioning theory, these spiked sediment values are equivalent to interstitial water LC50 values of 47.28 μg/L to C. xanthus and LOEC of 7.24 μg/L to C. dubia. Considering the LOEC values to C. dubia obtained in the present work, it could be suggested that such effects are likely to occur in the environment, since current environmental concentrations of TCS in sediment have been reported in order from mg.kg-1. Moreover, when taken into account the equilibrium partitioning theory, the effect values are in a range of environmental concentrations reported in others studies. Since the sediments act as a secondary source of contaminants to aquatic environments by natural phenomena and anthropogenic activities, there is a necessity for more studies to determine the effects of TCS in sediments, as well as assessments on the bioavailable fraction of this compound at environmentally relevant concentrations. Keywords: Triclosan, ecotoxicity, spiked sediment Sociedade Brasileira de Ecotoxicologia (SBE) Universidade Federal de Pernambuco (UFPE) 471