Brazilian Collection of Environmental and Industrial Microorganisms Coleção Brasileira de Micro-organismos de Ambiente e Indústria - (CBMAI) Curator Dr. Derlene Attili de Angelis / [email protected] Address: Coleção Brasileira de Micro-organismos de Ambiente e Indústria (CBMAI), Divisão de Recursos Microbianos (DRM) - CPQBA/UNICAMP Rua Alexandre Cazellato, 999 Vila Betel - Paulínia - SP - CEP 13148-218– Brasil Telephone: +55 (19) 2139-2894 / 2895 FAX: +55 (19) 2139-2852 http://webdrm.cpqba.unicamp.br/cbmai/ Support anp Agência Nacional do Petróleo About CBMAI The Brazilian Collection of Environmental and Industrial Microorganisms - CBMAI was established in January 2002 at the Chemical, Biological and Agricultural Multidisciplinary Research Center (CPQBA) with the support of the State University of Campinas (UNICAMP), São Paulo Research Foundation (FAPESP), Ministry of Science, Technology and Innovation (MCTI) and Research and Projects Financing (FINEP). CBMAI is a service collection with the mission of acting as a Biological Resource Center (as defined by the Organisation for Economic Cooperation and Development - OECD), dedicated to the preservation, storage and distribution of microorganisms and its associated information as well as the taxonomic characterization and identification. CBMAI staff has expertise in microbiology and systematics of bacteria and fungi, with large experience in polyphasic taxonomy, molecular systematics, phylogeny and application of culture-independent methods for the microbial diversity characterization in environmental and industrial samples (eg. water, soil, oil, raw materials and manufactured products). Consultancy can be offered on quality system documents related to Culture Collections with focus on the NBR ISO/IEC 17025:2005 and OECD Best Practice Guidelines for Biological Resource Centers. Collection and Information System CBMAI collection holds microbial strains for biotechnological, educational and taxonomic (reference and type strains) applications and it contains biological material restricted to risk groups 1 and 2 (according to the classification of the World Health Organization - WHO), which include bacteria, fungi and yeasts. Genetically modified organisms (GMOs) are accepted for depositing when belonging to risk groups 1 or 2 according to the Brazilian classification (CTNBio). CBMAI data management uses the software micro-Sicol (Collections Information System) which was developed specifically for microbial collections and which permits creating the online catalogue (http://webdrm.cpqba.unicamp.br/catalogo) and its periodical updating. Services Deposit of Cultures: CBMAI is continuously increasing its collection, encouraging the deposit of microbial strains from either research projects or of industrial and environmental importance. Deposit requests are previously analyzed to confirm the biosafety risk level of new strains in accordance with those accepted by the Collection. OGMs may be accepted if they attend the biosafety restriction. CBMAI offers three types of deposit: open (public access), confidential (restricted deposit) and for legal purposes (compliance with Brazilian legislation, MP2186-16, with respect to access and delivery of microorganisms for research, bioprospecting and technological development). Supply of Cultures CBMAI distributes microbial strains for public and private institutions which has infrastructure and trained staff for handling microorganisms. CBMAI strains are preferably distributed as lyophilized ampoules or living cultures, when necessary. Characterization and Identification CBMAI offers characterization and identification services for bacteria, filamentous fungi and yeasts based on molecular and conventional taxonomy. The isolates can be identified using rDNA sequencing, fingerprinting, phylogenetic analysis, morphology, colony patterns, biochemical tests, etc. Microbial analyses at infra-specific level are being developed through Randomly Amplified Polymorphic DNA method RAPD, mainly for the typing of yeasts of industrial importance. Culture independent methods may be contracted, as well as the construction and analysis of genomic libraries. Curator Dr. Derlene Attili de Angelis / [email protected]