rd
23 Congress of the International Union for Biochemistry and Molecular Biology
th
44 Annual Meeting of the Brazilian Society for Biochemistry and Molecular Biology
th
th
Foz do Iguaçu, PR, Brazil, August 24 to 28 , 2015
CHEMICAL COMPOSITION OF AQUEOUS EXTRACT OF Campomanesia
xanthocarpa
Sant’Anna, L. S.1,2; Merlugo, L. 1,2; Santos, M.C.3,4; Ehle, C. 1; Limberger, J. 1; Blanco,
M. 1; Mendez, A.S.L.5; Moreira, C. M.1,2
1
Grupo de Pesquisa em Fisiologia Cardiovascular; Universidade Federal do Pampa,
RS, Brazil.
2
Programa de Pós-graduação em Bioquímica, Universidade Federal do Pampa,
Uruguaiana-RS, Brazil.
3
Laboratório de Desenvolvimento e Controle de Qualidade de Medicamentos,
Universidade Federal do Pampa, Uruguaiana - RS, Brazil.
4
Programa de Pós-graduação em Ciências Farmacêuticas, Universidade Federal do
Pampa, Uruguaiana - RS, Brazil.
5
Universidade Federal do Rio Grande do Sul, Porto Alegre - RS, Brazil.
INTRODUCTION: Popular traditional reports the use of guabiroba (Campomanesia
xanthocarpa) tea leaves for several diseases, mainly for digestive and dyslipidemia.
Studies demonstrated the presence of components such as flavonoids, saponines,
tannins and terpenes in guabiroba leaves, which could explain these properties. The
objective was to analyze the chemical composition of the aqueous extract of C.
xanthocarpa. MATERIAL AND METHODS: The leaves were collected, selected and
dried at 40 °C for five days, and the extract was prepared by infused with water at 80
°C. The extract was evaluated for total phenolic by the colorimetric method of FolinCiocalteau and the results are expressed in milligrams of Gallic Acid Equivalent per
milliliter ( mgGAE/mL). The total flavonoid content, expressed in Rutin Equivalent (RE),
was determined by spectrophotometric method based on the formation of complexes
with aluminum chloride solution. The chemical components were determined by
chromatography method in HPLC, using reverse phase system, gradient elution with
flow rate of 1 mL/min, mobile phase consisted of acetonitrile (5% v/v) and acetonitrile
(50% v/ v) containing 0,05 % phosphoric acid (85%) , injection volume of 40 µL, and
with DAD detection. RESULTS: The total phenolic content was 3.7360 mgAGE/mL,
since the content of total flavonoids was RE 2.5070 mg/g. In the chemical identification,
the results were based on comparison of the profile with patterns, suggesting the
presence of chlorogenic acid, theobromine and quercetin, the latter at high
concentrations. CONCLUSION: Based on the results it can be concluded that the
leaves of C. xanthocarpa present relevant content of phenolics and flavonoids, also it
was found the presence of substances with known clinical potential that can assist in
the investigation of the pharmacological properties of this plant.
Key-Words: Campomanesia xanthocarpa, chemical composition, quercetin.
Brazilian Society for Biochemistry and
Molecular Biology (SBBq)