CELL CYCLE ARREST INDUCED BY QUERCETIN IN HUMAN TUMOR
XENOGRAFT MODEL
Maso V 1, Calgarotto AK1, Franchi Jr GC2, Nowill AE2, Vasallo J3, Latuf Filho P3;
Saad STO1.
1. Instituto Nacional de Ciência e Tecnologia do Sangue, Hemocentro Unicamp,
Universidade Estadual de Campinas (UNICAMP), Campinas-SP.
2. Centro Integrado de Pesquisas oncohematológicas na InfânciaCIPOI, Universidade Estadual de Campinas (UNICAMP), Campinas-SP.
3. Centro de Investigação em Pediatria – CIPED, Universidade Estadual de
Campinas (UNICAMP), Campinas-SP.
Quercetin is ubiquitously found in plants and plant food sources, several beneficial
health effects have been associated with the dietary uptake of this polyphenolic
compound. A variety of studies indicate a possible use of quercetin for cancer treatment
through its interaction with multiple cancer-related pathways. The underlying
mechanisms of myelodysplastic syndromes involve signal proteins and transcription
factors which gives the cell a growth advantage over its normal counterpart.
Accordingly, the goal of this work was to identify the quercetin effects using P39 cells
in xenografted mice. The myeloid cell line P39, derived from a patient with MDSchronic myelomonocytic leukemia, was inoculated subcutaneously in NOD.CB17Prkdcscid/J lineage mice and quercetin (120mg/Kg) was administrated once every four
days by intraperitoneal. After 21 days, the mice were sacrificed and the tumors were
removed for western blotting and immunohistochemistry analyzes. Quercetin treatment
reduced 31.6% of tumor volume compared to control. Quercetin induces cell cycle
arrest in G1 phase, with reduction of cyclin D and E expression and phosphorylation of
Rb. The p21 staining was increased compared to control. We did not observe any
significant difference in cyclin A, Cdk2, 4 and 6. The quercetin treatment in
xenotransplant model reduces the tumor development, with pronounced induction of
cell cycle arrest.
Agência Financiadora: FAPESP
Área 02: Farmacologia