July 25th- 28th, 2012 Rio de Janeiro, Brazil 1 Contents www.sbbc.org.br Scientific Content Welcome to ICCB 2012 SBBC Council and Committees ICCB 10th Annual Meeting Supporters Meeting at a Glance Pre-Meeting Educational Activities Wednesday Program Thursday Program Friday Program Saturday Program Travel Awards General Information Meeting Registration Meeting Policies Meeting resources Transportation and Hotel Map General Travel Information Important phone numbers RioCentro Convention Center Attendee Resources RioCentro Convention Center Floor Plans Exhibitors Exhibitor Listings Exhibit Hall Floor Plan Poster Information Poster sessions and assignment Presentation instructions Poster title list Authors Author Index 2 Welcome! Welcome to the heart of biomedical sciences! As important as the function of cells for life, cell biology is at the center stage of science nowadays, either through the promises in therapy strategies and biotechnology or through the development of new tools and concepts, not to forget its importance and influence on science education. The program is dense, explores the many aspects of this fascinating area, and counts on the contribution from internationally recognized experts. We would like to express our sincere gratitude to invited speakers, guests, participants, exhibitors and the staff working to the different committees. This is also a great opportunity to thank the Brazilian Society for Cell Biology (SBBC) and the International Federation of Cell Biology (IFCB) for the partnership, and the different government agencies and institutions that contributed for both organizational and financial aspects, especially FIOCRUZ. The ICCB2012 is a happy and timely coincidence between the International Congress on Cell Biology and the Congress of the Brazilian Society for Cell Biology, which was detected and worked out in 2004. By that time, SBBC hosted the Ibero-American Congress of Cell Biology (CIABIC) in Campinas, and it became clear that integration of Latin America should be strongly encouraged for the following years. In practical terms, ICCB2012 will gather more than 2000 participants, which is twice as large as SBBC biennial meetings. ICCB2012 is one of the sixty nine international events taking place in Rio in 2012, and the city will host the World Soccer Cup in 2014 and Olympic Games in 2016. All this only reinforces the vibrating environment that we will be in for the next four days. We hope that by putting this meeting together we will be able to contribute to the discussion and definition of Cell Biology for the next years and moreover, we intend to create a forum to highlight the importance of science development in a nation whose origin roots up the hills in the Wonderful City and increases the pathways and connections for its integration regionally. Have a great ICCB and enjoy your stay in Rio! Hernandes F Carvalho and Patricia Gama Co-Chairs ICCB 2012 3 th th 10 ICCB and 16 SBBC Meetings Co-Organizers The Brazilian Society for Cell Biology (SBBC) EXECUTIVE COMMITTEE President Vice-President Directors Secretary Treasurer Wilson Savino - Fiocruz, Rio de Janeiro Vilma R Martins – Hospital A C Camargo, São Paulo Marimélia Porcionatto - UNIFESP, São Paulo Patrícia Gama - USP, São Paulo Flavia A C Gomes - UFRJ, Rio de Janeiro Irene Yan - USP, São Paulo Marinilce F Santos - USP, São Paulo The International Federation for Cell Biology (IFCB) President Denys Wheatley - Aberdeen, Scotland, UK Vice-President Cheng-Wen Wu - NHRI, Taiwan Secretary General Hernandes F Carvalho - UNICAMP, Brazil Executive organization Av. das Américas 3500 – Bl. Hong Kong 3000 - Sl. 405 Le Monde Office - Barra da Tijuca - RJ - 22640-102 Tel.: 55 21 3326-3320 Fax: 55 21 2437-1483 www.interevent.com.br 4 Organizing Committee Co-Chairs Hernandes F Carvalho (State University of Campinas) International Federation for Cell Biology IFCB Patrícia Gama (University of São Paulo) Brazilian Society for Cell Biology SBBC Brazilian Committee Estela Bevilacqua (University of São Paulo) Flávia Gomes (Federal University of Rio de Janeiro) Irene Yan (University of São Paulo) Marinilce F Santos (University of São Paulo) Marimélia Porcionatto (Federal University of São Paulo) Milton Moraes (FIOCRUZ) Thereza Christina Barja-Fidalgo (University of Rio de Janeiro) International Contacts Europe: Anne Eichmann (College de France, France; Yale University, USA) US: Bechara Kachar (NIDCD, NIH, USA) Latin America: Gabriel Rabinovitch (Buenos Aires University, Argentina) Asia: Ken Wen Wu (NHRI, Taiwan) Australia: James Armitage (Monash University, Australia) Scientific Committee Bechara Kachar Betina Malnic Carla Collares Carlos Ramos Célia Regina Garcia Celuta Sales Alviano Cláudia Mermelstein Claudio Simon Constance Oliver Edna Kimura Emer Suavinho Ferro Enilza Espreafico Estela Bevilacqua Fábio Papes Fernando Costa e Silva Filho Flávia CA Gomes Glaucia Santelli Gustavo Amarante Mendes Hernandes F Carvalho Hugo Armelin Irene Yan Ivarne Tersariol Jorg Kobarg José Garcia Abreu José Mauro Granjeiro José Xavier Neto Klaus Hartfelder Luiz Renato França Manoel Costa Mari Sogayar Maria Célia Jamur Maria Isabel Cano Marimélia Porcionatto Marinilce F Santos Marlene Benchimol Mirian Jasulionis Nadja Souza-Pinto Paolo Meda Patrícia Bozza Patrícia Gama Renata Pasqualini Ricardo Guellerman Roger Chammas Ruy Jaeger Sang Won Han Sérgio Schenckman Silvana Allodi Vilma R Martins Vivaldo Moura Neto Wadih Arap Wanderley de Souza Wilma Kempinas Wilson Savino 5 The organizers gratefully acknowledge the Financial Support FCW Fundação Conrado Wessel Institutional Support 6 The organizers gratefully acknowledge Exhibitors and Sponsors Fairport Biolince INFABIC- INCT 7 Program at a Glance July 25th (Wednesday) Room # 201 Cell Migration Marcelo Lamers 09h00- 10h30 202 204 205 Image J Cell culture as Transcriptional A public domain alternative model regulation & for image for animal transcriptome processing and experimentation analyses analysis Silvya S MariaKlaus Hartfelder Ruy Jaeger Engler & Silvia Berlanga 10h30- 10h45 12h00 12h15 Plant Cell Biology Adriana S Hemerly 207 208 209 212 Neurobiology signaling and plasticity in glial cells Flávia Gomes Advanced Microscopy Manoel Costa Muscle cell differentiation Claudia Mermelstein & Cécile GauthierRouviere (SBBC & French Society for Cell Biology) Cellular and molecular tools for invertebrate models Silvana Allodi Neurobiology signaling in glial cells Advanced Microscopy Muscle cell differentiation Cellular and molecular tools for invertebrate models Cooffe Break Cell Migration 10h45- 12h15 Courses 206 Image J analysis Cell culture as alternative model for animal experimentation Transcriptional regulation & transcriptome analyses Plant Cell Biology Exhibits open Lunch 8 12h30 Room # 12h30- 14h00 14h00-15h00 201 202 204 Special Interest Activities 205 206 Round Table: Publishing in Cell Biology Roger Chammas 12h45-14h45 SBBC Workshop: Can universities help schools? Marimélia Porcionatto L #1 L #2 Claudio Joazeiro Daria M-Rosen Neurodegeneration Metabolic stress L #3 Mark Ellisman Frontiers in Microscopy Imaging L #4 Y Shav-Tal Single gene tracking 17h30 17h30 12h45-14h45 Can universities help schools? L #5 Célia R Garcia Host Parasite Interaction: Malaria 208 209 12h45-14h45 IFCB Workshop: Scientific Writing Denys Wheatley 12h45-17h00 Workshop: Creative Cell Biology in schools Luiz Anastacio Alves 12h45-14h45 IFCB Workshop: Scientific Writing 12h45-17h00 Workshop: Creative Cell Biology in schools Coffee Break 15h00 15h30-17h00 207 Symp #1 Prions Jerson Lima e Silva Symp #2 Symp #3 Symp #4 Programs, Gene Therapy Cell Biology and Genes, and Martin Bonamino Reproduction Homeostasis Luiz Renato José Xavier Neto França Symp #5 Host Parasite Interaction Wanderley de Souza 12h45-17h00 Workshop: Creative Cell Biology in schools Exhibits close Opening Ceremony (Main Hall) Keynote Conference Elaine Fuchs 9 July 26th (Thursday) Room # 201 202 204 205 207 208 Symp #6 Membrane biology José Garcia Abreu Symp #7 Signaling in Development Ricardo G P Ramos Symp #8 Epithelial Proliferation & Differentiation Mari Sogayar Symp #9 Immune Cell Biology Wilson Savino Symp #10 Cell Biology and Education Bruce Alberts & Cynthia Jensen (American Society for Cell Biology & IFCB) Symp #11 Glia Club Vivaldo Moura Neto & Bernardo Castellano 08h45 - 10h15 10h00 Exhibits open Break Keynote Conference (Main Hall) Douglas Green 10h15 10h45- 11h45 11h45-13h45 Room # 12h15-13h45 13h00-14h00 Room # 14h00-15h00 Pavillion 5 (1st and 2nd floors) / 1st Poster Session Poster Presentation 11h45-12h45 even nrs Poster presentation 12h45-13h45 odd nrs 201 202 Special Symp Selected abstracts (Professionals) Special Symp Selected abstracts (Graduate students) Exhibitors - technical conferences 205 GE Healthcare Keynote Conference (Main Hall) Bruce Alberts Room # 204 IFCB General Assembly 207 Nikon 208 Life Technologies 10 Room # 15h15-16h15 201 202 204 205 207 L #6 Juan Bonifacino Polarized Sorting in neurons L #7 Anne Eichman Guidance of vascular patterning L #8 Hans Clevers Intestinal stem cell L #9 Mauro Pavão Targeting proteinglycan interactions L #10 Alejandro Schinder Neurobiology Coffee Break 16h15-16h45 16h45-18h15 208 Symp #12 Protein Folding and Assembly Carlos Ramos Symp #13 Vascular Cell Biology Robson Monteiro Symp #14 Cell Cycle control mechanisms Hugo Armelin & Patrícia Gama Symp #15 Migration and Regeneration Fernando Costa e Silva Filho Symp #16 Inflammation Patricia Bozza Symp #17 Glia Flávia Gomes 207 208 Exhibits close Keynote Conference (Main Hall) Ruslan Medzhitov 18h00 18h30-19h30 July 27th (Friday) Room # 201 Symp #18 Cancer therapy Jorg Kobarg 08h45 - 10h15 10h00 10h15 10h45- 11h45 11h45-13h45 202 204 Symp #19 Symp #20 Regulators of Tissue Regeneration neural transmission Juan Larrain Vilma Martins & Roy Larson 205 Symp #21 Metabolic Programming James Armitage Symp # 22 Symp #23 Mitochondria Cytotoxicity Nadja Souza-Pinto Sandra Azevedo & & Enilza Espreafico Tamara Lah Turnsec Exhibits open Break Keynote Conference (Main Hall) Daniel St Johnston Pavillion 5 (1st and 2nd floors) / 2nd Poster session Poster Presentation 11h45-12h45 even nrs Poster presentation 12h45-13h45 odd nrs Room # 204 SBBC General Assembly 11 Room # 12h15-13h45 201 202 Special Symp Selected abstracts (Undergraduate students) Special Symp Selected abstracts (Graduate students) Exhibitors - technical conferences 205 Carl Zeiss 13h00-14h00 Room # Room # 14h15-15h15 201 202 204 L #11 Miriam Jasiulionis Epigenetics and malignant transformation L #12 Stefan Linder Podosomes, microtubules and motor proteins L #13 Marcelo Morales Bone-marrow stem cell therapy Symp #24 Cancer Renata Pasqualini 17h30-18h30 18h00 208 Roche 205 207 208 Symp #27 Maternal interface Estela Bevilacqua Symp #28 Cells as biosensors Glaucia Santelli & Paulo Saldiva Special Symp Oral Presentations (Extra Session) Coffee Break 15h15-15h45 15h45-17h15 207 BD Symp #25 Cell motility James Sellers Symp # 26 RNA regulation Jean Pierre Perrault (Canadian Cell Biology Society) & Carla C Oliveira Keynote Conference (Main Hall) Jennifer Lippincott-Schwartz Exhibits close 12 July 28th (Saturday) 9h00 Room # 9h00-10h00 Exhibits open 201 202 204 205 L# 14 Rick Horwitz Mechanosensing through myosin II L# 15 Stephen Doxsey Mitotic centrosomes in asymmetric events L# 16 Andrzej Bartke Growth hormone and Aging L #17 Sérgio Ferreira Neurodegenerative disorders Coffee Break 10h00 10h30-12h00 Symp # 29 MMPs and TIMPs Ruy Jaeger Symp #30 Telomeres Maria Isabel Cano Symp #31 Cancer Stemness Ken Wu (Taiwan Society for Cell and Molecular Biology) 12h00 Exhibits close Lunch 12h00 Room # 13h00 14h15- 15h15 15h15-15h45 Symp #32 Unconventional organelles Marlene Benchimol 201 202 204 L #18 Peter Friedl Cell migration L# 19 Xavier Belles MicroRNAs and metamorphosis L #20 Rafael Linden Prions Closing conference (Main Hall) Richard Hynes Closing remarks (Main Hall) 13 GENERAL INFORMATION ATTENDEE AND EXHIBITOR REGISTRATION Wednesday, July 25th 7h30-17h30 Thursday, July 26th 8h00-18h00 Friday July 27th 8h00-17h00 Saturday July 28th 8h00-16h00 SBBC MEETING MANAGEMENT/BUSINESS OFFICE Registration counter and Exhibition Hall 14h00- 17h00 MEDIA DESK & VIP ROOM- 2nd floor Room 210 8h00- 17h00 BADGES/REPLACEMENT POLICY Meeting badges must be worn at all times while in RioCentro Convention Center. Children over the age of 12 must wear a badge. There is a R$ 30,00 charge for lost or misplaced badges. Photo identification will be required for replacement. To avoid these charges, please remember to bring your meeting badge and materials with you. CAMERAS Cameras and other recording devices are prohibited in Poster Sessions. DRINKING AND SMOKING POLICIES The SBBC and IFCB encourage responsible drinking for those drinking alcohol. Coffee and water will be offered at Coffee breaks. Alcoholic beverages are allowed only in specific areas. According to Rio de Janeiro State Law 5.517 it is prohibited to smoke in any area at any public area, including the Convention Center. Food Court A Food Court will be available during meeting hours and will be organized with different restaurants. EXHIBIT HALL HOURS Wednesday Thursday and Friday Saturday 12h00- 17h30 10h00-18h00 9h00- 13h00 14 GROUND TRANSPORTATION In Rio de Janeiro, public transportation includes buses, subway and trains. Ticket costs around R$ 3,00. An integrated special ticket (bilhete único) can be used both in buses and subway. A special bus service runs from the Airports (Galeão and Santos Dumont) to specific sites in the city. For more information: www.rioonibus.com https://www.cartaoriocard.com.br/scrcpr/ For bus routes check: http://www.vadeonibus.com.br/vadeonibus/index.php Taxis are yellow in Rio de Janeiro, and there are two other companies working as red and blue. There will be a bus service to and from Riocentro, see the map below. LOST AND FOUND AND MESSAGE CENTER Please contact Registration Desk for lost and found. Messages for invited speakers and/or attendees should be left at ICCB registration desk. POSTER SESSIONS Poster Sessions will be held at 1st and 2nd floors (Room 203) and will be organized according to the different areas (informed below). Poster Session I: Thursday, July 26th Poster Session II: Friday, July 27th Author presentation for both sessions: 11h45- 12h45 even numbers 12h45-13h45- odd numbers Poster numbers will identify the boards. Tapes and hangers should be brought to the area by presenters. The Organizing Committee will not provide these items and will not collect and keep Posters that are left on the Boards. SAFETY AND SECURITY Rio de Janeiro is a large city and care should be taken as in any other huge city. We are committed to make the necessary efforts to ensure a safe, productive and nice event for everyone. Please remember to take off your badge when exiting the Convention Center. Please be aware of your surroundings at all times. For emergencies while in RioCentro, contact a uniformed security officer. For emergencies while in your hotel, please follow the specific instructions. WEATHER July monthly highs average 25o C, lows average 15o C. It is usually a dry season in Rio de Janeiro. 15 Transportation and hotels Two differents Routes will be serving the Congress. Transportation System Routes Blue Route: from Barra da Tijuca Beach Stops Praia Linda, Windsor Barra, Sheraton Barra and Casa del Mar Red Route: From Avenida das Américas Barra First and Bourbon Residence Guests staying at the hotels: Paradiso All Suites, Transamérica Barra and Royalty Barra must take the Blue Route transportation. Departures to Rio Centro (Blue Route and Red Route) July 25th Beetween 07:15 | 07:30 Between 12:00 | 12:15 July 26th Between 07:30 | 07:45 Between 09:00 | 09:15 July 27th Between 07:30 | 07:45 Between 09:00 | 09:15 July 28th Between 08:00 | 08:15 Between 09:30 | 09:45 Between 16:30 | 16:45 Return from Rio Centro (Blue Route and Red Route) July 25th and July 26th 19:45 July 27th 18:45 July 28th 15:45 (*) (*) On this day, there will be transportation departuring from Rio Centro to the Internacional Airport (Galeão) and Santos Dumont Airport, in this order. A free bus will be running from Barra Shopping (BS) to Riocentro (RC) and back at hourly schedule (starting at 12h30 at BS on July 25th and 8h00 (BS) on the following days). For more information contact the registration desk. 16 More on travelling information BARRA DA TIJUCA Barra da Tijuca is Rio´s most modern living complex and community; sophisticated, vibrant and offering innumerable attractions such as fine bars and restaurants serving world class cuisine, airconditioned shopping malls featuring world famous fashions and designers labels, theme parks, ecological reserves and sports of all types. Only 15 km from Ipanema Beach and 18 km from Copacabana this part of the city is on continued expansion and is becoming Rio’s business and economic center extended by miles of an outrageous virgin beach. While in Rio don’t miss the opportunity to discover the city. PASSPORT & VISAS Passports are required for all foreigners. Brazil’s visa requirements are based upon reciprocity. If your home country requires a visa for Brazilian travelers, you will need one for your visit. Additional information can be obtained from the nearest Brazilian Embassy or Consulate. Your passport expiration date should be at least six months from the date of your arrival. For more information, please visit http://www.passportsandvisas.com/visas/brazil-visa-faq.asp HEALTH There are no compulsory health requirements for entry into Brazil. We suggest that you contact your local Consulate for current advice. Please note that if you are entering Brazil via Colombia, Equador or Peru, you will be required to provide a current yellow fever vaccination certificate for immigration purposes. MEDICAL AND INSURANCE SERVICES Rio de Janeiro and Brazil have a number of internationally respected hospitals, clinics and doctors, but treatment is costly, so visitors are strongly advised to take out medical trip insurance. The Congress Organization is not liable for any health problems, personal accidents, lost baggage or cancellation of travel arrangements, flights, etc. We recommend that participants provide their own insurance policies. FOOD & DRINKS The most common dishes feature various meats, rice and the ubiquitous Brazilian black beans (feijão), while restaurants many times offer all-you-can-eat barbecues and buffets. Many kinds of alcoholic drinks are available, including excellent lager style beers such as Antarctica, Brahma, Cerpa and Skol. The most popular local beverage is Cachaça, most commonly served as 'Caipirinha' with slices of lime or lemon. There are no restrictions on licensing hours. Soft drinks include Guarana (a carbonated cola-like drink, made from the Amazon fruit, guarana) and many varieties of fruit juices (sucos). Brazilian coffee tends to be served less strong than Italian coffee, so if stronger coffee is desired, request express coffee (café expresso). If you would like to avoid sugar in juices or coffee, you should specifically request that it not be added. FOREIGN EXCHANGE The Brazilian monetary unit is the Real (R$). Exchange rates are published daily in the newspaper. Cash and traveler checks, especially US Dollars (USD), can be exchanged at most banks, exchange houses and major hotels. • Bank notes (paper money) are in denominations of R$ 100, R$ 50, R$ 10, R$ 5, R$ 1. • Coins are 1.00 real, 50 centavos (cents), 25 centavos, 10 centavos and 5 centavos. • Banking Hours - 10:00-16:00 Monday to Friday. 17 TIPPING In most restaurants and bars, a 10% service fee is added to the bill. More sophisticated places may add 15%. If service is not included, it will be stated at the bottom of the bill: “Serviço não incluído.” Airport and hotel porters: the R$ is equivalent to the USD of $1.00 per suitcase. Hotels: Hotels generally include any service charge on the bill. Restaurants: Tips are discretionary, but often found on the final bills as a "suggestion." In Brazil, a typical tip remains 10%. Taxis: Tips are not expected by taxi drivers although most passengers will round the fare up if satisfied with the service SOME LAST ADVICES TO ENJOY RIO Below, are a few general recommendations to help you enjoy a safe and relaxing trip to Rio de Janeiro: Never leave your luggage unattended or with a stranger and please be aware that some may attempt to create a distraction to divert your attention from your belongings. The sun in Brazil can be more direct and stronger - extra precautions are necessary Be aware of dangerous undertows; stay near other bathers and observe the warning flags. Do not go to the beach at night. Avoid wearing expensive jewellery or watches, carry limited amounts of cash and keep your passport and other important travel documents at your hotel. When not in use, it is advised that you carry your camera in your pocket and as a precaution, be sure to carry your bag in front of you. IMPORTANT PHONE NUMBERS Brazil code: 55 Rio de Janeiro code 21 Police 190 Emergency and Fireman 193 Internacional Airport 0800999099 Domestic Airport (Santos Dumont) 0800244646 Riocentro 3035 9100 18 Meeting will be held at RioCentro Convention Center Av Salvador Allende 6555, Barra da Tijuca, Rio de Janeiro, RJ, 22780-160 Brazil Google maps At Riocentro ICCB is at Pavillion 5 You are here Pavillion 5 19 Pavillion 5 1st floor Exhibitors Ambriex AOTEC BD Biosciences Biogen Biolince Biometrix Carl Zeiss Fairport FUNPECPeprotec GE Healthcare INFABIC- INCT Inopat John Wiley Life Technologies Lonza Merck Millipore Nikon Nobilis Tur Nova Analítica Olympus Perkin Elmer Promega Roche SBBC SBS Livraria Sigma Spectrun Pavillion 5 2nd Floor 20 Pre-meeting activities SBBC Educational program Workshop “Experiencing Biology” June 23rd, 2012 Escola Estadual Leda Guimarães Natal (Parelheiros, São Paulo, SP) Coordination: Profa Dra Marimélia Porcionatto (UNIFESP) Advanced Optical Microscopy Course July 16th- 20th Universidade Federal do Rio de Janeiro, UFRJ Coordination: Prof Dr Manoel Luis Costa Wednesday, July 25 Courses 9h00- 12h15 (Coffee Break 10h30- 10h45) Room 201 Cell Migration Marcelo Lamers, Peter Friedl, Alan Horwitz Room 202 Image J: A public domain for image processing and analysis Ruy Jaeger Room 204 Cell culture as alternative model for animal experimentation Silvya Stuchi Maria-Engler, Silvia Berlanga Room 205 Transcriptional regulation & transcriptome analyses Klaus Hartfelder Room 206 Plant Cell Biology Adriana Silva Hemerly, Marcelo Dornelas Room 207 Neurobiology signaling and plasticity in glial cells Flávia Gomes, Arturo Ortega, Ricardo A Melo Reis, Adan Aguirre, Marcelo Santiago Room 208 Advanced Microscopy Manoel Costa, Clarissa Henry, John Murray, João Menezes Room 209 Muscle cell differentiation Cláudia Mermelstein, Cécile Gauthier-Rouviere (SBBC & SFBC) Room 212 Cellular and molecular tools for invertebrate models Silvana Allodi, Cintia M Barros, Dib Ammar, Rodrigo Fonseca, Juliana Américo 12h00 – Exhibits open 21 Round Table and Workshops 12h30- 14h00 Room 201 Special Interest Subgroup Round Table: Publishing in Cell Biology Chair Roger Chammas Bruce Alberts, Fiona Watt 12h45- 14h45 Room 206 SBBC Workshop: Can universities help schools? Marimélia Porcionatto Room 208 IFCB Workshop: Scientific Writing Denys Wheatley 12h45- 17h00 Room 209 Workshop: Creative Cell Biology in schools Luiz Anastácio Alves, Flávia Lima, Daniela Uziel Rozental Lectures 14h00- 15h00 Room 201 L# 1 Claudio Joazeiro The Scripps Research Institute, La Jolla, USA The ribosome-associated E3 ubiquitin ligase, Listerin (Ltn1), is implicated in neurodegeneration and mediates a novel pathway of protein quality control Chair: Marilene H Lopes Room 202 L# 2 Daria Mochly-Rosen Stanford University, USA Excessive mitochondrial fission mediated by PKC and by Drp1 activation; new targets for neuroprotection Chair: Déborah Schechman Room 204 L# 3 Mark Ellisman University of California San Diego, USA New approaches for correlated LM and 3D EM applied to MULTISCALE CHALLENGES: Bridging Gaps in Knowledge and Understanding Chair: Marcia Attias 22 Room 205 L# 4 Yaron Shav-Tal Bar-Ilan University, Ramat Gan, Israel Dynamics of gene expression in real-time measured on single genes in single living cells Chair: Jean Pierre-Perreault Room 207 L#5 Célia Regina Garcia Universidade de São Paulo, Brasil PfCBF transcription factor, a new player for signal transduction in melatonin-pathways in malaria parasites Chair: Sérgio Schenkman 15h00- Coffee Break Symposia and Special Interest Subgroups 15h30- 17h00 Room 201 Symp #1 Special Subgroup Prion protein in physiology and pathology Chair: Jerson Silva Vilma Martins Hospital A.C. Camargo, São Paulo, Brasil Neurodegeneration and cancer: a crosstalk between prion protein and its ligand STI1 David Harris Boston University School of Medicine, Boston, USA Neurotoxic Activities of PrPC in Prion and Alzheimer’s Diseases Jerson Silva Federal University of Rio de Janeiro, Rio de Janeiro, Brazil Room 202 Symp #2 Special Subgroup Programs, Genes and Homeostasis Chair: José Xavier Neto Michael Schubert Université de Lyon, France Retinoic acid signaling in development and evolution Kleber Franchini LNLS, Campinas, Brazil José Xavier Neto LNLS, Campinas, Brasil Ancient programs of gene expression in development and homeostasis 23 Room 204 Symp #3 Special Subgroup Gene Therapy Chair: Martin Bonamino Luigi Naldini Director, San Raffaele Telethon Institute for Gene Therapy (HSR-TIGET), Italy A Hematopoietic Stem Cell (HSC) Specific microRNA Gives Novel Insights into the Regulation of HSC Homeostasis and Allows Safer HSC-based Gene Therapy Rafael Linden Universidade Federal do Rio de Janeiro, Brazil Fundamentals of a novel approach to gene therapy of glaucoma Martin Bonamino Instituto Nacional de Cancer, Rio de Janeiro, Brazil Conditional models for Chimeric Antigen Receptor (CAR) based activation of T lymphocytes Room 205 Symp #4 Special Subgroup Cell Biology & Reproduction Chair: Luiz Renato França Richard Sharpe University of Edinburgh, UK Fetal testis differentiation and function, its regulation and its disorders Maria Christina Werneck Avellar Universidade Federal de São Paulo, Brazil Antimicrobial Proteins Secreted by the Epididymis Luiz Renato França UFMG, Belo Horizonte, Brazil Spermatogonial stem cell niche in vertebrates Room 207 Symp #5 Special Subgroup Host Parasite Interaction Chair: Wanderley de Souza Wanderley de Souza Universidade Federal do Rio de Janeiro (UFRJ), Brazil Introductory notes Sérgio Schenkman Universidade Federal de São Paulo (UNIFESP), Brazil Dephosphorylation of eIF5A is Required for Translation Arrest at the Stationary Growth Phase of Trypanosoma cruzi Kiaran Kirk The Australian National University, Canberra, Australia Ion Regulation in the Malaria Parasite: The Target of a New Generation of Antimalarials Michel Rabinovitch Universidade Federal de São Paulo (UNIFESP), Brazil Why coinfect cells with non-viral pathogens? 24 Keynote Conference 17h30 Opening Cerimony Main Hall Opening Conference Elaine Fuchs The Rockefeller University, New York, USA Skin Stem Cells in Homeostasis, Wound Repair and Cancer Chair: Estela Bevilacqua Thursday, July 26 Symposia 8h45- 10h15 Room 201 Symp #6 Membrane Biology Chair: José Garcia Abreu Christophe Lamaze Institut Curie, Paris, France Membrane Dynamics and Mechanics of Signaling: Role of Caveolae Derek Toomre Yale University School of Medicine, USA Studying spatial control of exocytosis at the nanoscale in living cells José Garcia Abreu Universidade Federal do Rio Janeiro, Brazil New inhibitory Wnt/β-catenin mechanisms affecting embryonic head formation Room 202 Symp #7 Signaling in Development Chair: Ricardo Guellerman Pinheiro Ramos Roeland Nusse Stanford University, USA Wnt Signaling, Stem Cells and Tissue Repair Olivier Pourquié Institut de genétique et biologie moleculaire et celulaire, INSERM, France Formation of the Vertebrate Body Axis Matthew Scott Stanford University, USA Hedgehog Signaling in Development and Disease Room 204 Symp #8 Epithelial proliferation and differentiation Chair: Mari Sogayar Fiona Watt CRUK Cambridge Research Institute, Li Ka Shing Centre, UK Intrinsic And Extrinsic Regulation Of Epidermal Stem Cell Fate 25 João Viola Program of Cellular Biology, Instituto Nacional de Câncer (INCA), Rio de Janeiro, RJ, Brazil. Differential roles for NFAT transcription factor isoforms in cell transformation Mari Sogayar Department of Biochemistry, Chemistry Institute, University of São Paulo, São Paulo, Brazil Room 205 Symp #9 Immune Cell Biology Chair: Wilson Savino Flávio Salazar Onfraya University of Chile Immunological and Clinical Outcomes of a New DC-Based Vaccine Augustin G Zapata Faculty of Biology, Complutense University, Spain Eph/ephrin-mediated interactions govern functional maturation of developing thymocytes in the thymic epitelial 3D network Wilson Savino Instituto Oswaldo Cruz, FIOCRUZ, Rio de Janeiro, Brazil Room 207 Symp #10 Cell Biology and Education (ASCB & IFCB Symposium) Chairs: Bruce Alberts and Cynthia Jensen Cynthia Jensen University of Auckland, New Zealand Cell Biology and Education Marlene Behchimol Universidade Santa Úrsula and Fundação CECIERJ, Rio de Janeiro, Brasil Teaching at Distance: Interactive Multimedia of the Cell Biology of Trypanosoma cruzi Kiaran Kirk The Australian National University, Canberra, Australia Engaging undergraduate students in research Room 208 Symp #11 Glia Club Chairs: Vivaldo Moura Neto and Bernardo Castellano Arthur Butt University of Portsmouth, U.K. GSK3β is a profound negative regulator of oligodendrocyte differentiation and myelination Geoff Pilkington University of Portsmouth, UK Role of cancer stem cells in adult and paediatric brain neoplasms: hoax or holy grail? Tamara Lah Turnsec National Institute of Biology, Ljubljana, Slovenia Mesenchymal Stem Cells lower proliferation and invasion of Glioblastoma cells, exploiting the Immune Response Mediating Chemokines 26 Bernardo Castellano Universidade Autonoma de Barcelona Effects of CNS-targeted Il-6 or Il-10 production on microglial activation and motor neuron degeneration after facial nerve axotomy 10h00 – Exhibits open 10h15- 10h45 – Break Keynote Conference 10h45- 11h45 Main Hall Douglas Green St. Jude Children's Research Hospital, USA Cell death Chair: Wilson Savino st 1 Poster Session 11h45- 12h45 Pavillion 5 (1st and 2nd floors) Poster Presentation - even numbers 12h45- 13h45 Pavillion 5 (1st and 2nd floors) Poster Presentation - odd numbers 11h45 – IFCB General Assembly – Room 204 Symposia- Selected Abstracts 12h15- 13h45 Room 201 Special Symposium Professional selected abstracts Hiroshi Hosoya,Hiroshima University Hinrich P Hansen University Clinic Cologne, Köln, Germany Simone Vargas da Silva, Universidade do Estado do Rio de Janeiro Danielle Pereira Cavalcanti, Inmetro Patricia V. Burgos, Universidad Austral de Chile Chairs: Maria Inês Borella, Flávia Lima and Graciela Dutari 27 Room 202 Special Symposium Graduate students selected abstracts- Session I Andrew Oliveira Silva, Universidade Federal do Rio Grande do Sul Gabriela Nana Colaneri, Universidade Federal de São Paulo, UNIFESP Priscila Teles de Tolêdo Bernardes, UFMG Luciana Pescatore, FM USP Diego Pinheiro Aguiar, ICB, UFRJ Chair: James Armitage, Sérgio L Felisbino Exhibitors Technical Conferences and Activities 13h00- 14h00 Room 205 GE Healthcare Room 207 Nikon Room 208 Life Technologies Keynote Conference 14h00- 15h00 Main Hall Bruce Alberts University of California, San Francisco, USA Science, Biology, and the World’s Future Chairs: Wanderley de Souza and Denys Wheatley Lectures 15h15- 16h15 Room 201 L# 6 Juan Bonifacino National Institutes of Health, Bethesda, USA Signal-Adaptor Interactions that Mediate Polarized Sorting in Neurons Chair: Luis Lamberti Silva Room 202 L# 7 Anne Eichmann Yale University, New Haven, USA Guidance of vascular patterning: lessons from the nervous system Chair: Luiz Eurico Nasciutti Room 204 L# 8 Hans Clevers University Medical Centre Utrecht, Utrecht, the Netherlands Lgr5 Stem Cells in self-renewal and cancer Chair: Vivaldo Moura Neto 28 Room 205 L# 9 Mauro Pavão Universidade Federal do Rio de Janeiro, Brasil Targeting protein-glycan interactions at cell surface during EMT and hematogeneous metastasis: consequences on tumor invasion and metastasis Chair: Marimélia Porcionatto Room 207 L# 10 Alejandro Schinder Leloir Institute, Buenos Aires, Argentina Adult-born neurons contribute to information processing in the dentate gyrus Chair: Flávia Gomes 16h15- 16h45 – Coffee Break Symposia 16h45- 18h15 Room 201 Symp #12 Protein Folding and Assembly Chair: Carlos Ramos Douglas M Cyr UNC-Chapel Hill, USA Mechanisms for folding corrector action in rescue of mutant CFTR from premature degradation by ER Quality Control Alberto Macario Istituto Euro- Mediterraneo di Scienza et Tecnologia, Palermo, Italy Chaperonopathies: Impact on protein folding and beyond Francisco Laurindo University of São Paulo, Brazil Redox Processes Associated with Physicological Protein Folding and Endoplasmic Reticulum Stress Room 202 Symp #13 Vascular cell biology Chair: Robson Monteiro Joseph H McCarty University of Texas, Houston, USA Cell Adhesion and Signaling Pathways in Neurovascular Developmen Jean Leon Thomas Brain and Spinal Cord Institute, Paris, France Vascular growth factor signaling in neurogenesis Robson Monteiro Institute of Medical Biochemistry, UFRJ, Brazil Tumor-Derived Microvesicles and their Role in Cancer Progression 29 Room 204 Symp #14 Cell cycle control mechanisms Chairs: Hugo Armelin and Patricia Gama Leslie I Gold New York University Stabilizing nuclear p27kip1 with Skp2/Cks1 E3 ligase inhibitors as a potential therapeutic intervention for endometrial cancer and other cancers Michele Pagano New York University, USA Cyclin F-mediated degradation of RRM2 (Ribonucleotide Reductase family member 2) controls genome integrity and DNA repair Guido Lenz Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil Resveratrol and Temozolomide co-treatment induces mitotic catatrophe and senescence in glioma cells through modulation of mitotic regulators Room 205 Symp #15 Migration and regeneration Chair: Fernando Costa e Silva Filho Mark Ginsberg University of California, San Diego, USA Inside- out integrin signaling Tatiana Coelho-Sampaio UFRJ, Rio de Janeiro, Brasil Human-laminin mediates axonal regeneration promoted by human adipose tissue-derived stromal cells after spinal cord injury in rats Fernando Costa e Silva Filho UFRJ, Rio de Janeiro, Brasil A mechanochenical cross-talking between eukaryotic cells and their surroundings instruct cells on what they have to do Room 207 Symp #16 Inflammation Chair: Patrícia Bozza Niels O S Câmara University of Sao Paulo, Brazil The intimate link between fibrosis and inflammatory response Richard Bucala Yale University, New Haven, USA How a Parasite MIF Suppresses T cell Immunity and Influenced the Evolution of Macrophage Responsivenes Patrícia Bozza Instituto Oswaldo Cruz, FIOCRUZ, Brazil Room 208 Symp #17 Glia Chair: Flavia A C Gomes Arturo Ortega Cinvestav-IPN, México DF, Mexico GLAST/EAAT1 induces Glutamine release through SNAT3 in cultured chick cerebellar Bergmann glial cells 30 Frank Pfrieger University of Strasbourg, France Understanding Neuron-Glia Interactions: Models Matter Adan Aguirre Stony Brook University, USA NG2+ glial cells participate in normal brain physiology and thedevelopment of depressive-like behaviors 18h00 – Exhibits close Keynote Conference 18h30- 19h30 Main Hall Ruslan Medzhitov Yale University, New Haven, USA Inflammation: Physiology, Pathology and Evolution Chair: Patricia Bozza Friday, July 27 Symposia 8h45- 10h15 Room 201 Symp #18 Perspectives in cancer therapies Chair: Jörg Kobarg Atanasio Pandiella Universidad de Salamanca, Spain Deciphering Neuregulin-HER signaling in breast cancer Jörg Kobarg Centro Nacional de Pesquisa em Energia e Materiais, Campinas, Brasil Prospecting and testing new molecular target proteins for cancer therapy: integrating systems and structural biology Room 202 Symp #19 Regulators of neural transmission Chairs: Vilma Martins and Roy Larson Jeremy M Henley University of Bristol Regulation of neuronal function and dysfunction by protein SUMOylation Christina Joselevitch University of São Paulo, Brazil Gain control in the outer retina Martin Cammarota Instituto de Pesquisas Biomédicas, PUCRS, Porto Alegre, Brazil 31 Room 204 Symp #20 Tissue Regeneration Chair: Juan Larrain Ken Poss HHMI, Duke University Medical Center, Durham, USA Cellular and molecular mechanisms of zebrafish heart regeneration José Garcia-Arrarás University of Puerto Rico Cellular and molecular mechanisms of intestinal regeneration in echinoderms Juan Larrain Pontificia Universidad Catolica de Chile Spinal cord regeneration in Xenopus Room 205 Symp #21 Metabolic Programming Chair: James A Armitage Patricia Lisboa State University of Rio de Janeiro, Brazil Smoking in the postnatal life and future obesity: the nicotine role on the endocrine dysfunctions Licio Velloso University of Campinas, Brazil Diet-induced hypothalamic inflammation in obesity James A Armitage Monash University, Victoria, Australia Maternal obesity, diabetes or high fat intake in pregnancy: Are they all independent risk factors for metabolic syndrome in her offspring? Room 207 Symp #22 Mitochondria Chairs and Speakers: Enilza Espreafico and Nadja Souza-Pinto Nadja Souza-Pinto Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, SP, Brazil Mitochondrial BER activities maintain mtDNA stability and mitochondrial function Alicia Kowaltowski Universidade de São Paulo, Brazil Dietary interventions, mitochondria, oxidants and lifespan Enilza Espreafico Universidade de São Paulo, Ribeirão Preto, Brazil Evidence implicating KIAA0090/CG2943 in mitochondrial function Room 208 Symp #23 Cytotoxicity Brazilian-Slovenian Meeting Chairs: Sandra Azevedo and Tamara Lah Turnsec Bojan Sedmak National Institute of Biology, Ljubljana, Slovenia Cyclic cyanopeptides influence cytoskeleton organization in glial cells 32 Gregor Anderluh University of Ljubljana, Slovenia Equinatoxin effects on cellular membranes 10h00- Exhibits open 10h15- 10h45- Break Keynote Conference 10h45- 11h45 Main Hall nd 2 Daniel St Johnston University of Cambridge, UK Polarizing perpendicular axes in Drosophila Chair: Irene Yan Poster Session 11h45- 12h45 Pavillion 5 (1st and 2nd floors) Poster Presentation - even numbers 12h45- 13h45 Pavillion 5 (1st and 2nd floor) Poster Presentation - odd numbers 11h45 – SBBC General Assembly – Room 204 Symposia- Selected Abstracts 12h15- 13h45 Room 201 Special Symposium Undergrad students selected abstracts Wesley Luiz Barros da Silva, Fluminense Federal University Luana De Santana Bottas, Biosciences, University of São Paulo, São Paulo, Brazil. Lucas Antonio Duarte Nicolau, UFPI Brenno Vinícius Martins Henrique, University of Brasília Flavio Augusto Rocha Barbosa, Universidade Federal de Santa Catarina Chairs: Ivarne Tersariol, Regina Goldenberg 33 Room 202 Special Symposium Graduate students selected abstracts María Eugenia Sabatino, Universidad Nacional de Córdoba, Argentina Eugenio Damaceno Hottz, IOC, Fiocruz Manuela Weitkunat, Max Planck Institute of Biochemistry Clarissa Xavier Resende Valim, FMRP, USP Rebeca Piatniczka Iglesia, ICB, Universidade de São Paulo, Brasil Chairs: Christina Barja-Fidalgo, Paola Casanello, Giselle Zenker Justo Exhibitors Technical Conferences and Activities 13h00- 14h00 Room 205 Carl Zeiss Room 207 BD Room 208 Roche Lectures 14h15- 15h15 Room 201 L# 11 Miriam Jasiulionis Ontogeny and Epigenetics Laboratory, Pharmacology Department, Universidade Federal de São Paulo, São Paulo, Brazil Epigenetic alterations: Linking sustained stress to melanocyte malignant transformation Chair: James Armitage Room 202 L# 12 Stefan Linder University Medical Center Eppendorf, Hamburg Regulation of macrophage podosomes by microtubules and motor proteins Chair: Fernando Costa e Silva Filho Room 204 L# 13 Marcelo Morales Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, UFRJ, Rio de Janeiro, Brasil Bone marrow-derived stem cell therapy attenuates lung silicosis and lung fibrosis Chair: Ken Wu 15h15- 15h45 – Coffee Break 34 Symposia 15h45- 17h15 Room 201 Symp #24 Cancer Chair: Renata Pasqualini Webster Cavenee University of California San Diego, USA Tumor Cell to Tumor Cell Interaction Drives Cancer Heterogeneity Wadih Arap The University of Texas, M D Anderson Cancer Center, USA Targeting Adipose Tissue to Prevent Cancer Progression Renata Pasqualini The University of Texas, M D Anderson Cancer Center, USA Integration of in Vivo Phage Display & Targeted nanotechnology and Moleculargenetic Imaging Room 202 Symp #25 Cell motility Chair: James Sellers Paul Selvin University of Illinois, USA Single Molecule Fluorescence and Optical Trapping Applied to Molecular Motors: Two can do it better than one. Marie-France Carlier Cytoskeleton Dynamics and Motility group, Gif-sur-Yvette, France Microfluidics pushes forward microscopy analysis of actin dynamics James Sellers National Institutes of Health, Bethesda, USA A Tale of Two Tails: The Regulation of Myosin-5a and Myosin-7a Room 204 Symp #26 RNA regulation Canadian Cell Biology Society Chairs and Speakers: Jean-Pierre Perreault and Carla Columbano Richard B Pearson University of Melbourne, Australia Inhibition of RNA Polymerase I as a Strategy to Treat Cancer Carla Columbano Oliveira Universidade de São Paulo, Brasil Identification of proteins regulating the RNA exosome Jean-Pierre Perreault Université de Sherbrooke, Canada Impact of G-quadruplex structures on the human transcriptome Room 205 Symp #27 Maternal interface Chair: Estela Bevilacqua Felipe Vadillo-Ortega Universidad Nacional de Mexico (UNAM), Mexico 35 Paola Casanello Faculty of Medicine, Pontificea Universidad Católica de Chile, Chile The placenta as an early marker of genomic, proteomic and epigenetic changes involved in vascular diseases Graciela Panzetta Universidad Nacional de Córdoba, Argentina Expression and function of PSG, StarD7 and KLF6 genes in human trophoblast cells Room 207 Symp #28 Cells as biosensors Chairs: Paulo Saldiva and Glaucia Santelli Cecília Verônica Nunez Instituto Nacional de Pesquisas da Amazônia, Manaus, Amazonas, Brazil Cytotoxic indole alkaloids isolated from Duroia macrophylla (Rubiaceae) Paulo Saldiva University of São Paulo, Brazil Cellular responses to ambient levels of air pollution Glaucia Santelli University of São Paulo, Brazil Cell-fiber interactions: effects on cell biology Room 208 Special Symposium – Extra Session Bárbara Hissa de Carvalho Vieira Couto, Universidade Federal de Minas Gerais, Brasil Ana Lúcia Vargas Arigony Corte, Universidade Federal do Rio Grande do Sul, Brasil Daniel Moreira Silva, Universidade Federal de Uberlândia, Brasil Raphael Silveira Vidal, Universidade Federal do Rio de Janeiro, Brasil Eduardo Cremonese Filippi Chiela, Universidade Federal do Rio Grande do Sul Samyra Maria dos Santos Nassif Lacerda, Universidade Federal de Minas Gerais Chair: Marimelia Porcionatto Keynote Conference 17h30- 18h30 Main Hall Jennifer Lippincott-Schwartz Kennedy Shriver NICHHD, NIH, Bethesda, MD 20892 Navigating the cellular landscape with new optical probes, imaging strategies and technical innovations Chair: Nadja Souza-Pinto 18h00- Exhibits close 36 Saturday, July 28 Lectures 9h00- 10h00 Room 201 L# 14 Rick Horwitz Department of Cell Biology, University of Virginia School of Medicine Mechanosensing Through Myosin II: From Migration to Learning and Memory Chair: Christina Barja-Fidalgo Room 202 L# 15 Stephen Doxsey University of Massachusetts Medical School Emerging roles of mitotic centrosomes in asymmetric events and cilia disorders Chair: Glaucia M M Santelli Room 204 L# 16 Andrzej Bartke Department of Internal Medicine, Southern Illinois University School of Medicine, Springfield, Illinois, USA Growth Hormone and Aging; Benefits of Endocrine Defects Chair: Luiz Renato França Room 205 L# 17 Sérgio Teixeira Ferreira Institute of Biomedical Sciences, Federal University of Rio de Janeiro, Brazil Synapse failure induced by Alzheimer's toxic Aβ oligomers Chair: Bernardo Castellano 10h00- 10h30 – Coffee Break Symposia 10h30- 12h00 Room 201 Symp #29 MMPs and TIMPs Chair: Ruy Jaeger Stanley Zucker Stony Brook University, USA Membrane Type I- Matrix Metalloproteinase (MMP14): A Multifaceted Cell Surface Protease in Cancer Rama Khokha University of Western Ontario, Canada 37 Vincent Lagente UMR991 INSERM/Université de Rennes 1, France Role of matrix metalloproteinases and inflammasome pathway in the development of airway inflammation and fibrosis Room 202 Symp #30 Telomeres Chair: Maria Isabel Cano Maria Teresa Teixeira Institut de Biologie Physico-Chimique, France Saccharomyces cerevisiae as a model for the study of telomere-mediated replicative senescence Rodrigo Calado Universidade de São Paulo, Ribeirão Preto, Brazil Telomere dysfunction in human disease Maria Isabel Cano UNESP, Botucatu, Brazil Searching for a CST-like complex at Leishmania spp. telomeres Room 204 Symp #31 Cancer Stemness Taiwan CB Society Chair: Ken Wen Wu Tariq Enver University College London, UK Ken Wen Wu National Yang-Ming University, Taipei, Taiwan SOX2 promotes lung cancer stemness by inducing EGFR and BCL2L1 expression Room 205 Symp #32 Unconventional organelles Chair: Marlene Benchimol Martin Embley Newcastle University, UK Reductive evolution and the minimal mitochondria of microsporidian parasites Kildare Miranda Federal University of Rio de Janeiro, Brazil Dynamic control of the contractile vacuole complex and acidocalcisomes and their functional role in the mechanisms of regulatory volume decrease in Trypanosomatid parasites Ulysses Casado Lins Federal University of Rio de Janeiro, Brazil Cell biology of magnetotactic bacteria and their organelles: the magnetosomes Marlene Benchimol Universidade Santa Úrsula, Rio de Janeiro, Brazil An unconventional organelle: the hydrogenosome 38 Lectures 14h15- 15h15 Room 201 L# 18 Peter Friedl Radboud University Nijmegen Medical Centre, The Netherlands Collective cancer invasion, tissue guidance, and plasticity of therapy response Chair: Marinilce F Santos Room 202 L# 19 Xavier Belles Institute of Evolutionary Biology (CSIC-UPF), Barcelona Regulation of insect metamorphosis and the role of microRNAs. Nepenthe teams up with Psyche Chair: Klaus Hartfelder Room 204 L# 20 Rafael Linden UFRJ, Rio de Janeiro, Brazil The prion protein as a prototypical cell surface scaffold protein Chair: Vilma Martins Keynote Conference 14h15- 15h15 Main Hall Richard Hynes MIT, Cambridge, USA Extrinsic influences on tumor progression - platelets and extracellular matrix Chair: Hernandes F Carvalho 15h15- Main Hall- Closing Remarks 39 Travel Awards Scientific Committee Flavia C A Gomes (Federal University of Rio de Janeiro) Milton Moraes (FIOCRUZ) Hernandes de Carvalho (State University of Campinas) Coordination: Amanda Araujo (Interevent) Selected Students Affiliation Country Adny H. Silva Amado Quintar Andrés Nilson Caniuguir Ortega Anita Mayara Feitosa Santos Bernardo Javier Krause Leyton Carla Evelyn Coimbra Nunez Eder Carlos Schmidt Everton de Brito Oliveira Costa Universidade Federal de Santa Catarina (UFSC) Universidad de Córdoba Perinatology Research Laboratory (PRL) Universidade Federal do Ceará (UFC) Perinatology Research Laboratory (PRL) Universidade Estadual de Campinas (UNICAMP) Universidade Federal de Santa Catarina (UFSC) Centro Regional de Hemoterapia de Ribeirão Preto Universidade Federal de Santa Catarina (UFSC) EMBRAPA / CENARGEN Centre de Recherche en Oncologie biologique et Oncopharmacologie Hiroshima University Universidad Nacional de Córdoba Universidade Federal de São Paulo (UNIFESP) Life and Health Sciences Research Institute (ICVS), School of Health Sciences, University of Minho Institute of Molecular and Cell Biology Universidade Federal do Pará (UFPA) Universidade Federal do Pernambuco (UFPE) Universidade Federal do Ceará (UFC) Universidade Federal do Rio Grande (FURG) Brazil Argentina Chile Brazil Chile Brazil Brazil Brazil Flavio Augusto Rocha Barbosa Graziella Anselmo Joanitti Luciana Pescatore Alves Makiko Morita María Eugenia Sabatino Mariana Cristina Cabral Silva Olga Catarina Lopes Martinho Patrícia Renck Nunes Paula Cristina Rodrigues Frade Paulo Euzébio Cabral Filho Priscila Briseno Frota Renata Ottes Vasconcelos Brazil Brazil France Japan Argentina Brazil Portugal Singapore Brazil Brazil Brazil Brazil 40 Keynote Conferences- Abstracts Skin Stem Cells in Homeostasis, Wound Repair and Cancer Cell death Elaine Fuchs Howard Hughes Medical Institute, The Rockefeller University, New York, NY, USA 10065 Douglas Green St. Jude Children's Research Hospital, USA Embryonic skin begins as a single layer of unspecified epithelial cells. During development, these cells receive external cues to undergo a series of morphogenetic events which culminate in the production of a stratified epidermis replete with hair follicles, sebaceous glands and sweat glands. Postnatally, each of these tissues undergoes self-renewal which requires stem cells. We’ve demonstrated that there are distinct populations of resident stem cells within epithelial tissues. How these cells develop and how they balance self-renewal and differentiation is of fundamental importance to our understanding of normal tissue maintenance and wound repair, and to elucidate how the balance of growth and differentiation goes awry in cancers, particularly squamous cell carcinomas, among the most prevalent and life-threatening of human cancers. Using skin as our paradigm, we’ve been dissecting how extrinsic signaling to stem sets off a cascade of changes in transcription that governs the activation of stem cells during tissue development, homeostasis, hair cycling and tumorigenesis. Our findings have provided us with new insights into our understanding of the process of stem cell activation, and in so doing have revealed mechanisms which are also deregulated in a variety of different human cancers. In this talk, I will review some of our studies that implicate a complicated cross-talk between stem cells and their niche microenvironment, and how these communication circuitries change in normal homeostasis and wound repair and in tumor progression. Science, Biology, and the World’s Future Inflammation: Physiology, Pathology and Evolution Bruce Alberts Professor Emeritus of Biochemistry and Biophysics, University of California, San Francisco; Editor-in-chief, Science magazine; United States Science Envoy Ruslan Medzhitov, Ph.D. David W. Wallace Professor of Immunobiology Investigator, Howard Hughes Medical Institute Yale University School of Medicine New Haven, CT USA There are many exciting challenges ahead for biologists. Living organisms are so complicated that we will need new methods of analysis to achieve any deep understanding of their molecular mechanisms. For example, even when we have determined each of the hundreds of different molecular interactions that create the actin cytoskeletal system, and know the three-dimensional structures and rate constants for the formation and disassembly of each of its possible sub-complexes, the challenge of computing the outcomes will remain. In the same sense, most of the interesting properties of cells and organisms are “emergent properties”, resulting from a large network of interactions that have non-intuitive outcomes. More broadly, the knowledge and the problem-solving skills of scientists are critical for every nation – no matter how rich or poor. Thus, for example, science has produced a deep understanding of the natural world that often enables an accurate prediction of the consequences of current actions on the future. In addition, every society needs the values of science: honesty, generosity, and an insistence on evidence while respecting all ideas and opinions regardless of their source of origin. To spread such values, science education needs to be redefined at all levels, with much less emphasis on the memorization of science facts and terms. Instead, we should be providing empowering experiences in problem-solving that take advantage of the curiosity that children bring to school and increase a student’s understanding of the world. Closely related changes in the introductory science courses in college, emphasizing “science as a way of knowing,” are the key to driving these reforms. Inflammation is an adaptive response to noxious conditions that disrupt tissue homeostasis. The inflammatory response alters the functional state of target tissues and organs aiming to eliminate the inflammatory inducers and to restore homeostasis. This unavoidably occurs at the expense of normal tissue function thereby creating a potential for pathological alterations. In addition, inflammatory control mechanisms are generally antagonistic to the homeostatic control mechanisms. Therefore, the inflammatory response presents a fundamental trade-off between host protection and inflammatory pathology. This trade-off was optimized under environmental conditions that are no longer present for most modern human populations and was shaped by evolutionary priorities that are no longer relevant for most humans suffering from inflammatory diseases. Understanding the evolutionary and physiological roots of the inflammatory response will help to develop prophylactic and therapeutic better strategies for the prevention and treatment of modern human diseases. 41 Polarizing perpendicular axes in Drosophila Daniel St Johnston The Gurdon Institute, University of Cambridge, UK Almost all cells in a multicellular organism must be polarized to perform their normal functions, while a loss of polarity is a hallmark of cancer. Work over the last decade has revealed that a conserved set of polarity (PAR) proteins define complementary cortical domains in all polarized cell-types examined so far. How these complementary domains are established is much less well understood, however, with the exception of the C. elegans zygote. We have analyzed how the similar PAR domains form in the Drosophila oocyte to define the anterior–posterior axis (AP) of the embryo. Our results reveal that the oocyte is polarized by a different mechanism from the C. elegans zygote that also appears to operate in epithelial cells. Nevertheless, the organizing principles that underlie polarity seem to be conserved. The dorsal-ventral (DV) axis is also defined by the polarized organization of the oocyte, in this case by the movement of the nucleus from the posterior cortex of the oocyte to its anterior/lateral border. Although the movement of the oocyte nucleus was thought to depend on the prior establishment of AP polarity, we have isolated a mutant that separates these two processes. More importantly, live imaging reveals a novel mechanism of nuclear movement. This reveals that the oocyte has two parallel polarity systems and leads to a revised view of how orthogonal AP and DV axes form. Navigating the cellular landscape with new optical probes, imaging strategies and technical innovations Jennifer Lippincott-Schwartz Eugene Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892 Emerging visualization technologies are playing an increasingly important role in the study of numerous aspects of cell biology, capturing processes at the level of whole organisms down to single molecules. Recent developments in probes, techniques, microscopes and quantification are dramatically expanding the areas of productive imaging. Photoactivatable fluorescent proteins (PA-FPs) have been particular fruitful in this regard. They become bright and visible upon being exposed to a pulse of UV light. This allows selected populations of proteins to be pulselabeled and tracked over time. Used for in cellulo pulse chase experiments, the PA-FPs have helped clarify mechanisms for biogenesis, targeting, and maintenance of organelles as separate identities within cells. PA-FPs have further permitted the development of single molecule-based superresolution (SR) imaging, which dramatically improves the spatial resolution of light microscopy by over an order of magnitude (~10-20 nm resolution). Involving the controlled activation and sampling of sparse subsets of photoconvertible fluorescent molecules, single molecule SR imaging offers exciting possibilities for obtaining molecule scale information on biological events occurring at variable time scales. Here, I discuss the new fluorescent imaging techniques and the ways they are helping researchers navigate through the cell to unravel long-standing biological questions. Extrinsic influences on tumor progression - platelets and extracellular matrix Richard Hynes Howard Hughes Medical Institute, Koch Institute, MIT, Cambridge, MA 02139, USA. [email protected] Intrinsic changes in tumor cells contribute to metastatic potential, but influences from the surrounding environment also play important roles. We have shown that platelets actively promote invasive and malignant behavior of tumor cells by activating signal transduction pathways (TGF- /SMAD and NF B) within the tumor cells and enhancing invasive behavior, extravasation and metastasis. Platelets form aggregates around tumor cells that also recruit leukocytes, which further enhance malignancy. Alterations in extracellular matrix (ECM) occur during normal development and in pathologies such as fibrosis, skeletal diseases and cancer. Despite clear indications that tumor ECM and its interactions with cells play important roles in tumor progression, we do not have a good picture of ECM composition, origins and functions in tumors. One reason lies in the biochemical properties of ECM proteins (large size, insolubility, cross-linking, etc.) that render attempts to characterize ECM composition very challenging. We have developed proteomics-based methods coupled with bioinformatic definition of the “matrisome” (ECM and ECMassociated proteins) to analyze the protein composition of tissue extracellular matrices. We have characterized the ECMs of normal tissues and of non-metastatic and metastatic tumors. We have applied this approach to understand the origins of tumor ECM and shown that both tumor cells and stromal cells contribute to significant changes in the ECMs of tumors of differing metastatic potential. We have begun to apply this approach to human patient material to characterize the ECM composition of tumors of varying prognosis with the goal of developing ECM signatures that may be of diagnostic and/or prognostic value. 42 Lectures- Abstracts L#1 The ribosome-associated E3 ubiquitin ligase, Listerin (Ltn1), is implicated in neurodegeneration and mediates a novel pathway of protein quality control Claudio Joazeiro The Scripps Research Institute, La Jolla, CA mRNA lacking stop codons ('non-stop mRNA') can arise from errors in gene expression, and encode aberrant proteins whose accumulation could be deleterious to cellular function. In bacteria, these 'non-stop proteins' become co-translationally tagged with a peptide encoded by ssrA/tmRNA (transfermessenger RNA), which signals their degradation by energydependent proteases. How eukaryotic cells eliminate non-stop proteins remained unknown. We have recently reported that the S. cerevisiae Ltn1 RING-domain-type E3 ubiquitin ligase acts in the quality control of non-stop proteins (Bengtson & Joazeiro 2010. Nature 467:470-3). The Ltn1-mediated process is mechanistically distinct but conceptually analogous to that performed by ssrA: Ltn1 is predominantly associated with ribosomes, and marks nascent non-stop proteins with ubiquitin to signal their proteasomal degradation. Ltn1-mediated ubiquitylation of non-stop proteins seems to be triggered by their stalling in ribosomes on translation through the poly(A) tail. The biological relevance of this process is underscored by the finding that loss of Ltn1 function confers sensitivity to stress caused by increased non-stop protein production. We speculate that defective protein quality control may underlie the neurodegenerative phenotype that results from mutation of the mouse Ltn1 homologue, Listerin. In my talk, I will review these data and will present recent findings and further characterization of the Listerin/Ltn1 pathway. L#2 Excessive mitochondrial fission mediated by PKC and by Drp1 activation; new targets for neuroprotection Daria Mochly-Rosen1, Marie-Helene1 Distanik and Xin Qi1.2 1 Department of Chemical and Systems Biology, Stanford University School of Medicine, Stanford, CA, USA. 2Current address: Department of Physiology, Center for Mitochondrial Diseases, Case Western Reserve University School of Medicine, Cleveland, OH, USA Neuronal cell death in a number of neurological disorders is associated with aberrant mitochondrial dynamics and mitochondrial degeneration. However, the triggers for this mitochondrial dysregulation are not known. We found that activation of protein kinase C (PKC) induced aberrant mitochondrial fragmentation and impaired mitochondrial function in neurons in an in vivo rat model of hypertensive encephalopathy. We found that PKC directly bound to Drp1, a major mitochondrial fission protein, and phosphorylated it at Ser 579, thus increasing mitochondrial fragmentation. Importantly, inhibition of PKC, using a selective PKC inhibitor peptide that we have designed, reduced impaired mitochondrial fission and conferred neuronal protection in models of hypertensive encephalopathy. We also found that this PKC inhibitor reduced mitochondrial dysfunction in models of Parkinsonism. Together, we show that PKC activation dysregulates the mitochondrial fission machinery and increases ROS production, thus contributing to at least two neurological pathologies by increasing mitochondrial fission, fragmentation and dysfunction. Since PKC may be critical for other cellular functions, we next focused on generating an inhibitor of Drp1 interaction with Fis1. Applying a rationally designed approach, we identified P110, a peptide inhibitor of Drp1/Fis1 interaction, and show that this peptide is highly effective and selective in inhibiting aberrant mitochondrial fission in neurons and cell death induced by neurotoxins, such as those associated with Parkinsonism. Therefore, we suggest that inhibitors of aberrant mitochondrial fission might be useful for treatment of human diseases in which dysregulation of mitochondrial dynamics occurs. 43 L#3 New approaches for correlated LM and 3D EM applied to MULTISCALE CHALLENGES: Bridging Gaps in Knowledge and Understanding Mark H. Ellisman, Ph.D., Professor of Neurosciences and Bioengineering; Director, the National Center for Microscopy and Imaging Research (NCMIR) (http://www.ncmir.ucsd.edu/) UCSD. A grand goal in cell biology is to understand how the interplay of structural, chemical and electrical signals in and between cells gives rise to tissue properties, especially for complex tissues like nervous systems. New technologies are hastening progress as biologists make use of an increasingly powerful arsenal of tools and technologies for obtaining data, from the level of molecules to whole organs, and at the same time engage in the arduous and challenging process of adapting and assembling data at all scales of resolution and across disciplines into computerized databases. This talk will highlight projects in which development and application of new contrasting methods and imaging tools have allowed us to observe otherwise hidden relationships between cellular, subcellular and molecular constituents of cells, including those of nervous systems. New chemistries for carrying out correlated light and electron microscopy will be described, as well as recent advances in large-scale high-resolution 3D reconstruction with LM, TEM and SEM based methods. Examples of next generation cell-centric image libraries and web-based multiscale information exploration environments for sharing and exploring these data will also be described. L#4 Dynamics of gene expression in real-time measured on single genes in single living cells Yaron Shav-Tal The Mina & Everard Goodman Faculty of Life Sciences, Bar-Ilan University, Ramat Gan, Israel How can the transcriptional output of a gene be measured in a living cell? One approach is to measure transcriptional kinetics on multiple-copy gene-arrays by quantifying the rates at which fluorescently tagged mRNA is produced. This technique can provide accurate rates of transcription elongation in vivo. Another system allows the detection of transcriptional gene activity from a single gene, thereby providing the ability to analyze the kinetics of gene expression at the single allele level. This analysis can discern between endogenous and overexpressed states of a gene, and provide spatial and temporal information on transcription throughout the cell cycle. 44 L#5 PfCBF transcription factor, a new player for signal transduction in melatonin-pathways in malaria parasites L#6 Signal-Adaptor Interactions that Mediate Polarized Sorting in Neurons Wania Rezende Lima, Miriam Moraes and Célia R. S. Garcia Departamento de Fisiologia, Instituto de Biociências, Universidade de São Paulo – São Paulo- Brasil Ginny G. Farías, Loreto Cuitino, Xiaoli Guo, Xuefeng Ren, Rafael Mattera and Juan S. Bonifacino Cell Biology and Metabolism Program, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland, USA The signal transduction pathways controlling malaria parasite development remain largely unexplored. It is now accepted that Plasmodium senses the environment and exploits calcium and cAMP signalling pathways to modulate cellular functions. We want to understand how the molecular machinery for signalling transduction is put in action in Plasmodium, how second messengers are generated and if they play a role in the cell cycle. We have reported that potentially important signaling molecules from the host cell, the Red Blood Cell (RBCs) such as ATP modulates Plasmodium falciparum progression within RBCs through the rise of cytosolic Ca2+. We have used a cell-permeant form of caged-IP3 to investigate the cytosolic IP3 levels under physiological conditions in infected RBCs co-loaded with both the cell-permeant caged-IP3 and Fluo4-AM. UV flash photolysis of caged-IP3 under these conditions elicited a rapid and transient increase in intracellular Ca2+ in RBCs infected with P. falciparum. Thereby providing a direct evidence that a classical PLC-dependent intracellular Ca2+ release pathway operates in P. falciparum infected RBCs. We provided the first direct evidence that the host hormone melatonin elicits a rise in intracellular IP3 levels in the malaria parasite. We have also found that the Plasmodium kinase PfPK7 is central in the downstream mechanism for synchronizing the parasite as a P. falciparum clone unable to express PfPK7 does not respond to melatonin. Taken together these data implicate that melatonin activates Phospholipase C (PLC) to generate IP3 and open ER-localized IP3sensitive Ca2+ channels in P. falciparum In our search for the molecular effectors of second messenger signaling in P. falciparum we have search for the role of the PfCBF. The CBF family of transcription factors are involved in the regulation of cell cycle of many eukaryotic genes. The molecular and functional characterization of transcription factors in P. falciparum are yet poorly studied. In order to determine the protein and mRNA expression levels of intraerythrocytic PfCBF, western-blot and qRT-PCR were performed. To localize the CBF protein distribution in the parasite, confocal microscopy and subcellular fractionation were carried out. The effect cAMP on PfCBF gene and protein expression during intraerythrocytic development of the malaria parasites was followed by qRT-PCR and western blot analysis. PfCBF is expressed throughout the intra-erythrocytic stages but is greatly detectable at the schizont stage at both the mRNA and protein levels. In conclusion, We suggest that PfCBF may have an integral role in parasite melatonin responses and the subsequent parasite development. Then progress in understanding the function of PfCBF transcription factor in the context of the melatonin response is likely to provide a better knowledge of the parasite biology. Neurons are anatomically and functionally polarized cells that conduct nerve impulses in a vectorial fashion. Impulses are received by dendrites, propagated through the soma, and eventually transmitted to other cells by axons. The plasma membrane of each of these neuronal domains has a distinct protein composition, but the mechanisms responsible for this differential distribution of plasma membrane proteins remain poorly understood. By analogy to other protein sorting processes, we hypothesized that biosynthetic delivery of transmembrane proteins to different neuronal domains could be mediated by interaction of sorting signals in the cargo proteins with adaptor proteins that are components of protein coats. Indeed, we found that a tyrosine-based sorting signal in the cytosolic domain of the transferrin receptor (TfR) mediates sorting of this protein to the somatodendritic domain in both hippocampal and cortical neurons. This signal binds to the mu1A subunit of the clathrin-associated, heterotetrameric adaptor protein 1 (AP-1) complex. Overexpression of a dominant-negative mu1A mutant incapable of binding signals, or RNAi-mediated depletion of another subunit of the AP-1 complex, gamma-adaptin, resulted in missorting of the TfR to the axon. Various microscopic techniques revealed that sorting occurs at AP-1-coated areas of the TGN through exclusion of the TfR from transport carriers bound for the axon. These findings demonstrate that interactions of sorting signals with AP-1 mediate clathrin-dependent sorting of the TfR and other cargos to the neuronal somatodendritic domain. Together with recent observations in other polarized cell types, our findings support the notion that AP-1 is a global regulator of polarized sorting. 45 L#7 Guidance of vascular patterning: lessons from the nervous system Anne Eichmann Centre Interdisciplinaire de Recherche en Biologie (CIRB), Collège de France, Paris, France, Cardiovascular Research Center, Yale University School of Medicine, 300 George Street, New Haven, CT 06510-3221, USA Anatomical parallels between the nervous and the vascular system are readily apparent in peripheral body tissues, where blood vessels and nerves ramify throughout nearly all domains of the body and are usually aligned. Alignment of nerves and blood vessels allows the establishment of a physical relationship between them, as larger nerves are vascularized by vasa nervorum to ensure their oxygen and nutriment supply, while arteries are innervated by autonomic nerve fibers that control vascular tone. To orchestrate the formation of their highly branched, exquisitely wired networks, nerves and blood vessels have developed shared cellular and molecular principles. L#8 Lgr5 Stem Cells in self-renewal and cancer Hans Clevers Hubrecht Institute, Royal Netherlands Academy of Arts and Sciences & University Medical Centre Utrecht, Uppsalalaan 8, 3584 CT Utrecht, the Netherlands The intestinal epithelium is the most rapidly self-renewing mammalian tissue. Lgr5 is a gene transcribed in cycling, crypt base columnar cells at the crypt base. Using lineage tracing experiments the Lgr5+ve cells were identified as the stem cells of the intestinal epithelium. Furthermore, Lgr5+ve stem cells can initiate ever-expanding organoids in vitro. The Lgr5+ve stem cell hierarchy of differentiation is maintained in these organoids. Thus, intestinal crypt-villus units can be built from a single stem cell in the absence of a non-epithelial cellular niche. Although, Lgr5 stem cells persist life-long, crypts drift toward clonality quickly. The cellular dynamics are consistent with a model in which the stem cells divide symmetrically, and stochastically adopt stem or transient amplifying cell fates after cell division. Lgr5 stem cells are interspersed between differentiated Paneth cells, which produce all essential signals for stem-cell maintenance. Co-culturing of sorted stem cells with Paneth cells dramatically improves organoid formation. Genetic removal of Paneth cells in vivo results in the concomitant loss of Lgr5 stem cells. Intestinal cancer is initiated by Wnt pathway-activating mutations in genes such as APC. Deletion of APC in stem cells, but not in other crypt cells results in neoplasia, identifying the stem cell as the cell-of-origin of adenomas. Moreover, a stem cell/progenitor cell hierarchy is maintained in stem cell-derived adenomas, lending support to the “cancer stem cell”-concept. 46 L#9 Targeting protein-glycan interactions at cell surface during EMT and hematogeneous metastasis: consequences on tumor invasion and metastasis Mauro S. G. Pavao, Eliene O. Kozlowski and Felipe C. O. B. Teixeira Instituto de Bioquímica Médica, Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro, Brasil Two critical moments of carcinoma invasion and metastasis are the epithelial to mesenchymal transition (EMT), which occurs in the primary tumor and the hematogeneous metastasis, which occurs in the vascular system. Stromal growth factors and cell sulfate heparan sulfate proteoglycans (HSPG) are key mediators of EMT, whereas tumor cell surface glycans and P-selectin on activated platelets are essential for hematogeneous metastasis. Cell surface HSPG are co-receptors for the binding of several growth factors to their receptors involved in EMT and tumor dissemination. Two families of HSPGs carry the majority of the heparan sulfate on cells: glypicans and syndecans. Hematogeneous metastasis of cancer cells is a cascade of events involving the intravasation of tumor cells into the bloodstream, evasion of innate immune surveillance, adhesion to vascular endothelium of distant organs and colonization of tissues. During this process, the interaction of tumor cell surface glycans and platelet P-selectin creates complexes that allows tumor cells to evade the immune defenses and eventually colonize distant organs. Previous work from our lab suggest that the unique glycosaminoglycans from marine invertebrates may attenuate the response of tumor cells to growth factor–mediated EMT in the primary tumor and Pselectin-mediated formation of tumor cell-platelet complex during hematogeneous metastasis. As a consequence, tumor cell dissemination can be drastically reduced. L#10 Adult-born neurons contribute to information processing in the dentate gyrus Alejandro F. Schinder Laboratory of Neuronal Plasticity, Leloir Institute (IIBBACONICET), Buenos Aires The adult dentate gyrus generates new granule cells (GCs) that develop over several weeks and integrate into the preexisting network. While adult hippocampal neurogenesis has been implicated in learning and memory, the specific role of new GCs remains unclear. Is it solely the continuous addition of new neurons to the network what is important, or are there unique functional properties only attributable to new GCs that are relevant to information processing? While developing, immature GCs display elevated intrinsic excitability, reduced GABAergic inhibition and a capacity to undergo activitydependent synaptic potentiation. Such high intrinsic excitability would potentially allow immature GCs to be activated by entorhinal afferents in spite of their low density of glutamatergic inputs. It has thus recently been hypothesized that immature GCs might be critical to hippocampal function. We have recently examined whether immature adult-born neurons contribute to information encoding. Combining calcium imaging and electrophysiology in acute slices we found that weak afferent activity recruits few mature GCs while activating a substantial proportion of the immature neurons. These different activation thresholds are dictated by an enhanced excitation/inhibition balance that is transiently expressed in immature GCs. In addition, immature GCs exhibit low input specificity that switches with time towards a highly specific responsiveness. Therefore, activity patterns entering the dentate gyrus can undergo differential decoding by a heterogeneous population of GCs originated at different times. 47 L#11 Epigenetic alterations: Linking sustained stress to melanocyte malignant transformation L#12 Regulation of macrophage podosomes by microtubules and motor proteins Miriam Galvonas Jasiulionis Ontogeny and Epigenetics Laboratory, Pharmacology Department, Universidade Federal de São Paulo, São Paulo, Brazil Stefan Linder University Medical Center Eppendorf, Hamburg As other tumor types, both genetic and epigenetic alterations seem to contribute to melanoma genesis. Consistent evidences have suggested the key role of epigenetic marks in the genesis of pathologies induced by chronic stress. Increased levels of reactive oxygen species (ROS), caused for example by chronic inflammation, aging or UV radiation, might be responsible for abnormal epigenetic marks, which might significantly contribute to melanoma development. In this way, the study of the relationship among sustained stress, aberrant epigenetic marks and melanocyte malignant transformation may help to comprehend the mechanisms involved in melanoma genesis and, also, open new avenues to the development of new therapeutic strategies. Our laboratory established a murine model of melanocyte malignant transformation associated with sustained stress conditions. Progressive morphological and molecular alterations, which lead to the acquisition of malignant phenotype, were observed after submitting nontumorigenic melanocytes to sequential cycles of anchorage blockade. In this way, pre-malignant melanocytes corresponding to intermediate phases of malignant transformation (1C, 2C, 3C and 4C), and different melanoma cell lines, both non-metastatic (4C3- and 4C11-) and metastatic (4C3+, 4C11+, Tm1 e Tm5), were obtained from the nontumorigenic melanocyte lineage melan-a. Data from our group demonstrated that melan-a anchorage blockade results in oxidative stress and that increased levels of superoxide anion are related to global DNA hypermethylation and increased levels of Dnmt1 protein observed in this condition. We have been studied molecular mechanisms underlying alterations of epigenetic marks by ROS and the impact of this modulation on melanocyte malignant transformation. Podosomes are actin-based matrix contacts in a variety of cell types, most notably monocytic cells, and are characterized by their ability to lyse extracellular matrix material. Besides their dependence on actin regulation, podosomes are also contacted by microtubule plus ends and are influenced by microtubuledependent transport processes. This talk will highlight the role of microtubules and motor proteins in the regulation of podosome turnover and podosomal matrix degradation in primary human macrophages. A particular focus will be on the role of kinesin motors and the transport of the matrix metalloproteinase MT1-MMP. A further topic will be the differential regulation of podosome subpopulations, and especially the influence of the actomyosin system in podosome turnover. 48 L#13 Bone marrow-derived stem cell therapy attenuates lung silicosis and lung fibrosis L#14 Mechanosensing Through Myosin II: Learning and Memory Marcelo Morales Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, UFRJ, Rio de Janeiro, Brasil Rick Horwitz, Miguel Vicente-Manzanares, Lingfeng Chen, Jennifer Hodges, Karen Litwa, Kris Kubow, and Alexia Bachir Department of Cell Biology, University of Virginia School of Medicine Silicosis is an occupational disease produced by the deposition of silica particles in the lungs, which causes respiratory failure due to a fibrotic reaction. There is no effective treatment for silicosis, other than the cessation of the causative exposure. We showed in animal models of silicosis that bone marrow stem cell therapy is efficient to prevent the development of granulomas and attenuates the inflammatory process. In patients we reported the time course of lung perfusion scintigraphy of five patients with silicosis treated with intrabronchial instillation of autologous bone marrow derived mononuclear cells through bronchoscopy and this procedure showed to be safe with benefits to the lungs. These results open the opportunity for further studies in patients and possible use of this new technology for the treatment of lung silicosis. From Migration to Myosin II (MII) generates and interprets mechanical cues and thereby participates in a signaling loop that regulates diverse cellular processes. In cell migration, MII is a downstream target of Rho GTPases and major regulator of protrusion, adhesion, and polarity. We have recently identified new modes of MII regulation - novel tyrosine phosphorylation sites in the regulatory light chain and a cluster of phosphorylation sites in the tail region of the heavy chain - that modify its contractile and bundling properties. Most migration studies have utilized αvβ3 or α5β1 integrins, which serve as receptors for vitronectin and fibronectin. We have now extended this to other cell types and integrins. On the one hand myosin II plays an analogous role in the organization of dendritic spines and the postsynaptic density in hippocampal neurons during their development and response to stimulation. On the other, its role is greatly diminished in cells using the α6β1 or αLβ2 integrins. In these cells, the apparent affinity between integrin and its ECM leads to a novel molecular fluxing of integrins in adhesions. This results in reduced force transmission to the substrate, enhanced signaling, and increased directional migration. For cells migrating in 3D, the role of MII is modulated by the organization of the surrounding matrix resulting in the characteristic adhesion profiles observed in 3D. Finally, the effect of MII on adhesions is being studied using correlation microscopy and cryoEM. 49 L#15 Emerging roles of mitotic centrosomes in asymmetric events and cilia disorders Stephen Doxey University of Massachusetts Medical School , Department Program in Molecular Medicine University of Massachusetts Medical School 373 Plantation Street Worcester MA 01605 Mitosis is a fundamental process required for cell proliferation in all multicellular organisms. Much has been learned about the underpinnings of this process over the last century, but new and unexpected insights continue to be uncovered. Here we describe two novel and unanticipated findings associated with mitosis. The first is the unexpected identification of mitotic functions for proteins long known to function in cilia formation and ciliopathies. We show that the cilia proteins IFT88, IFT20 and IFT57 play a crucial role in the organization of spindle poles. Their depletion disrupts astral microtubules and misorients spindles. This has important implications for cystogenesis that accompanies ciliopathies. A second study shows that the midbody, a singular organelle formed between dividing daughter cells, is inherited by one daughter cell rather than being lost as a remnant or residual body as previously believed. Midbodies are inherited asymmetrically by the daughter cell with the older centrosome. Disruption of the older centrosome randomizes midbody inheritance. Midbodies accumulate in stem cells and cancer ‘stem cells’ but not in normal or differentiating cells. In differentiating cells midbodies are degraded by receptor-mediated autophagy; stem cells and cancer stem cells evade autophagic degradation. Midbody enrichment by blocking degradation enhances reprogramming to induced pluripotent stem cells and increases in vitro tumorigenicity of cancer cells. These results reveal unexpected post-mitotic roles for midbodies in stem cells and cancer ‘stem cells’. L#16 Growth hormone and aging; benefits of endocrine defects Andrzej Bartke Department of Internal Medicine, Southern Illinois University School of Medicine, Springfield, Illinois, USA Growth hormone (GH) levels progressively decline after reaching maximal levels in early adulthood and it was suspected that this decline may represent one of the causes of aging. Surprisingly, mice with mutations that cause GH deficiency and mice with targeted deletion of GH receptors live much longer than their normal siblings and exhibit symptoms of delayed aging. Extended longevity of these mutants is associated with reduced growth and adult body size, improved maintenance of pluripotent bone marrow stem cells, reduced incidence of cancer, increased fibroblast resistance to various cytotoxic stressors and various metabolic changes. Circulating levels of insulin and glucose are reduced and insulin sensitivity measured by insulin tolerance tests is enhanced. In GH receptor deleted (GHRKO) mice improved whole animal insulin sensitivity has been related to increased levels and phosphorylation of hepatic insulin receptors and reduced inhibitory (Serine 307) phosphorylation of IRS-1. mTOR signaling likely contributes to this change in IRS-1 phosphorylation. Results of surgical removal of intraabdominal adipose tissue indicate that adiponectin secreted by these fat depots enhances insulin sensitivity of long-lived GH-related mutants. Metabolic shifts in GH-deficient and GH-resistant mice also include increased oxygen consumption and reduced respiratory quotient, implying increased reliance on lipids as metabolic fuel. In sum, suppression of GH signals slows aging in mice by multiple mechanisms. Supported by NIA. 50 L#17 Synapse failure induced by Alzheimer's toxic Aβ oligomers Sérgio Teixeira Ferreira Biomedical Sciences Institute, Federal University of Rio de Janeiro (UFRJ), Rio de Janeiro, Brazil More than one hundred years after its original description, the mechanisms leading to memory loss and progressive cognitive impairment in Alzheimer’s disease (AD) remain controversial. Considerable evidence accumulated during the past decade implicates soluble oligomers of the amyloid-β peptide (Aβ), which accumulate in the brains of AD patients, as the proximal toxins that attack neurons and cause synapse failure culminating with memory impairment. This presentation will focus on mechanisms by which Aβ oligomers (AβOs) attack synapses and negatively impact the function of neuronal receptors important for synaptic plasticity. We recently showed that AβOs cause aberrant activation of NMDA receptors, which triggers dysregulation of intracellular Ca2+ levels, neuronal oxidative stress and receptor internalization. Similarly, AβOs induce removal of AMPA receptors from synapses. Along with changes in pre-synaptic neurotransmitter vesicle release, combined removal of NMDA and AMPA receptors from synapses may be part of the mechanism by which AβOs inhibit synaptic plasticity. We also demonstrated that NMDA receptors play a key role in the binding of Aβ oligomers to a neuronal receptor complex that putatively comprises additional protein components, among which the cellular prion protein. Finally, our recent work has shown that A Os inhibit neuronal insulin signaling, essential for neuronal survival, synaptic plasticity and memory formation. Elucidation of molecular/cellular mechanisms underlying the deleterious impact of AβOs on synapses may illuminate the development of novel therapeutic approaches to combat memory loss in AD. Key words: synaptotoxicity, amyloid- , memory loss L#18 Collective cancer invasion, tissue guidance, and plasticity of therapy response Peter Friedl1,2 1 Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands and 2 The University of Texas, MD Anderson Cancer Center, Houston, TX, USA The tumor microenvironment contributes to cancer invasion, growth and survival with impact on tumor response to therapy. We here employ intravital infrared multiphoton imaging for the multi-parameter visualization of cancer invasion, guidance by the tumor stroma, and therapy response. The data show predominantly collective cancer cell into the host stroma at speeds of up to 200 µm per day. Invasion resulted from nondestructive contact-guidance type migration exploiting preformed tracks of multi-interface topography, including 1D, 2D and 3D dimensionalities, but was independent of β1 and β3 integrin-mediated mechanotransduction. Collective invasion was coupled to altered survival capability, withstanding highdose radiotherapy and forming a resistance niche for subsequent relapse of the disease. Albeit invasion was integrinindependent, invasion-associated radioresistance was sensitive to the β1/β3 integrin targeting by RNAi or antiantibody treatment, resulting in anoikis induction and regression of both, tumor lesion and invasion strands. In conclusion, collective invasion is an important invasion mode in solid tumors that receive integrin signals from the tissue microenvironment for acquiring an altered phenotype and improved survival. 51 L#19 Regulation of insect metamorphosis and the role of microRNAs. Nepenthe teams up with Psyche Xavier Bellés Institute of Evolutionary Biology (CSIC-UPF), Barcelona E-mail: [email protected] Insect metamorphosis has fascinated mankind since the time of Aristotle, some two thousand years ago. But it was not until the decade of 1930 that the studies of Vincent B. Wigglesworth showed that insect metamorphosis is regulated by two hormones: the molting hormone, which induces the successive molts, and the juvenile hormone, which maintains the juvenile character of them. Then, a number of transcription factors act as mediators of these hormones, as well as a number of target genes that codify for proteins, giving the shape and behaviour of a juvenile or an adult stage. Working on the cockroach Blattella germanica, we found that microRNAs, which are RNAs of ca. 22 nucleotides that play a repressing action on mRNA, have a key role in metamorphosis. We silenced the expression of dicer-1 (a ribonuclease that mediates the maturation of microRNAs) in the last nymphal instar and the cockroaches, instead of molting to the adult stage, transformed into supernumerary nymphs. We presume that there are microRNAs that repress the expression of genes that maintain the juvenile status quo (like Nepenthe, the drug of forgetfulness of the Greeks). This leads to a change of genetic program, from juvenile to adult and, within the latter, there must be other microRNAs that modulate and refine the expression of genes that give rise to the adult animal, thus making it all right (like Psyche, the Greek symbol of transformation and new life). L#20 The prion protein as a prototypical cell surface scaffold protein. Rafael Linden Instituto de Biofísica da UFRJ, Rio de Janeiro, Brazil. Based on multiple interaction partners and pleiotropic signaling properties, we proposed the hypothesis that the prion protein (PrPC) is a cell surface scaffold protein, that serves as a dynamic platform for the assembly of signaling modules involved in widespread systemic functions. Our recent work is focused on 3 topics: (a) identification and validation of additional molecular interactions of PrPC; (b) structural evidence for allosteric function of PrPC; (c) functional properties and dysfunction of PrPC beyond the nervous system. A phage display screen, together with evidence from PrPC-null mice, implicate several neurotransmitter receptors and/or transporters in PrPCdependent signaling; Biophysical techniques showed that interaction of PrPC with its ligand hop/STI1 entails reciprocal structural remodeling, and strongly suggest allosteric effects that may be involved in the propagation of signals through PrPCmediated multiprotein complexes; Beyond the nervous system, we found that both peripheral inflammation and behavioral stress modulate the content of PrPC at the plasma membrane of neutrophils, with consequences upon peroxide-dependent cytotoxicity towards vascular endothelial cells. Our studies add to the understanding of both allosteric properties of the prion protein and systemic control of its expression and function. The data are consistent with the scaffold hypothesis that explains the multiple roles of the prion protein in physiology and pathology, and further suggest that PrPC may be relevant for clinically observed associations of stress and anxiety with either the severity or the progression of various degenerative/ noncommunicable diseases. (Supported by CNPq, FAPERJ, CAPES, FAPESP) 52 Symposia- Abstracts Symp#1 Prion protein in physiology and pathology Chair Jerson Silva Jerson Silva Federal University of Rio de Janeiro (UFRJ), Rio de Janeiro, Brazil Introductory notes Neurodegeneration and Cancer: a Crosstalk Between Prion Protein and its Ligand STI1 Vilma R. Martins Hospital A.C. Camargo, International Center for Research, Sao Paulo, Brazil C Prion protein (PrP ) is a highly abundant protein in the central nervous system. It’s misfolding is associated with fatal C neurodegenerative illnesses named prion diseases. PrP is known to bind to a number of extracellular or membrane proteins triggering specific signals that modulate diverse cellular functions. One of these ligands is Stress Inducible protein C 1 (STI1) whose interaction with PrP promotes neuronal survival, differentiation, neural progenitor/stem self-renewal and C memory formation. On the other hand, PrP transduces neurotoxic signals upon binding to A oligomers, the toxic C components in Alzheimer’s disease (AD). Our group has been exploring how the toxic signals triggered by PrP - A oligomers can be impaired by STI1. The results point that at least in vitro the toxicity of A oligomers can be reverted by C C recombinant STI1. Remarkable, PrP was described to participate in tumoral processes. Our results show that both PrP and STI1 are highly expressed in human glioblastomas (GBM) and their expression is associated with increased tumor C proliferation and decreased patient’s survival. In cell culture of GBM and in animal models the inhibition of PrP -STI1 C binding was able to inhibit the proliferation and also to increase animal survival. Therefore, the complex PrP -STI1 is an important therapeutic target in AD and in GBM. C Neurotoxic Activities of PrP in Prion and Alzheimer’s Diseases David A. Harris, Brian Fluharty, Jessie A. Turnbaugh, Tania Massignan, Ursula Unterberger, and Emiliano Biasini Dept. of Biochemistry, Boston University School of Medicine, Boston, MA 02118, USA C There is evidence that alterations in the normal physiological activity of PrP contribute to prion-induced neurotoxicity, and may also play a role in Alzheimer’s disease. This mechanism has been difficult to investigate, however, because the C normal function of PrP has remained obscure, and there are no assays available to measure it. We have found that cells expressing deletions or disease-associated point mutations in the conserved, central region of PrP exhibit spontaneous ionic currents that are likely due to unconventional channels or pores formed by the mutant PrP molecules themselves. These currents predispose neurons to excitotoxic death induced by endogenous, glutamatergic synaptic input. Current activity depends on the presence of a polybasic amino acid segment at the N-terminus (residues 23-31) that may serve as a tethered protein transduction domain capable of transiently permeabilizing the plasma membrane. The sequence C domains of PrP that are important for current activity are also critical for binding of oligomeric forms of the Alzheimer’s C Aβ peptide, suggesting that PrP may play a general role in mediating neurotoxicity in several neurodegenerative diseases, perhaps via abnormal activity of ion channels. 53 Symp#2 Programs, Genes and Homeostasis Chair José Xavier Neto Ancient Programs of Gene Expression in Development and Homeostasis José Xavier Neto LNLS, Campinas, Brazil The origins of vertebrate cardiac chambers among the simpler peristaltic pumps of invertebrate chordates are not understood. Peristaltic pumps operate by contractions that originate in the outside of a muscular conduit to squeeze its contents inside. Although versatile and adaptable, peristaltic pumps are limited by poor inflow-to-outflow coordination, often manifested by backflow, loss of fluid energy by distension, reflection and reversion. By breaking down circulatory work into dedicated inflow (reservoirs or atria) and outflow modules (pumps or ventricles), chambered hearts eliminated the inflow-to-outflow interference displayed by peristaltic pumps. This chambered mode of operation evolved only in vertebrates and in mollusks, which are animals that display simple tubular peristaltic pumps during early phases of their ontogenies, suggesting that chambered pumps evolved from ancestral peristaltic pumps. In amniotes such as mice and chicken, cardiac progenitors are divided by differential RA signalling into two broad anterior and posterior domains that will later give to outflow (ventricles and outflow tract) and inflow (sinus venosa and atria) cardiac tissues. This patterning mechanism has been modeled in a two-step process. Early specification signals are first conveyed by paracrine diffusion of the morphogen retinoic acid (RA) from posterior mesoderm towards posterior cardiac progenitors. Late determination signals are communicated by autocrine RA signaling setup by a caudal to rostral wave of raldh2 (aldh1a2), a gene encoding the main RA synthetic enzyme, which transiently confers posterior cardiac precursors with the ability to synthesize their own RA. Retinoic Acid Signaling in Development and Evolution Michael Schubert Institut de Génomique Fonctionnelle de Lyon (UMR 5242 du CNRS, Ecole Normale Supérieure de Lyon, Université Claude Bernard Lyon 1), Université de Lyon, 46 allée d’Italie, 69364 Lyon Cedex 07, France. E-mail: [email protected] Extensive research carried out in the course of the last 100 years has established that retinoids, which constitute a group of fat-soluble morphogens related to retinol (vitamin A), play crucial roles in early development, organogenesis, tissue homeostasis, proliferation, differentiation, and apoptosis. In vertebrates, most, but not all, retinoid functions are mediated by retinoic acid (RA) binding to heterodimers of two nuclear receptors: retinoic acid receptor (RAR) and retinoid X receptor (RXR). Retinoid signaling was long thought to be vertebrate-specific, but developmental studies in invertebrate chordates have revealed roles for retinoids conserved in all chordates. Outside chordates, however, evidence for functional roles of retinoids and of the RAR/RXR heterodimer remains scarce, although recent bioinformatic analyses have revealed that genes involved in retinoid signaling are present in the genomes of a variety of metazoan animals, including echinoderms (sea urchins) and lophotrochozoans (annelids and mollusks). These in silico results suggest that the retinoid pathway might have already been present in Urbilateria, the last common ancestor of protostomes and deuterostomes. To obtain insights into the diversification of the retinoid signaling cascade during morphological and genomic evolution, we have been using both developmental biology and bioinformatic approaches. We have thus addressed the question of the evolutionary origins of the retinoid signaling pathway and studied the molecular hierarchy controlled by retinoid signaling and its changes during evolution, with particular focus on the diversification of chordates. Altogether, our analyses have provided new insights into the origin and functional elaboration of the retinoid signaling pathway in the course of evolution. Kleber Franchini LNLS, Campinas, Brazil 54 Symp#3 Gene Therapy Chair Martin Bonamino Gene Therapy of Limb Ischemia with GM-CSF Sang Won Han Department of Biophysics, Federal University of São Paulo, São Paulo, Brazil Peripheral arterial diseases (PAD) affect about 1000 per million every year and about 200 of them suffer limb amputations. The PAD incidence increases in the case of diabetic patients and people with more than 60 years. Therefore, PAD has a high socio-economic and medical impact today. Approximately half of critical limb ischemic patients can be treated by vascular surgery, but the remainder depends mainly on the velocity of adaptation of the existing collateral vessels, a process known as arteriogenesis. Several growth factors, cytokines and proteases are required for arteriogenesis, which is the process of remodeling preexistent vessels during the ischemia. The monocytes, attracted by the presence of tumor necrosis factor-α and monocyte chemoattractant protein-1 at the inflammatory sites, are the main producers of factors for arteriogenesis. The time and concentration of the factors required to complete arteriogenesis varies in each case of PAD, consequently the action of the activated monocytes may not be enough to resolve the ischemic problem. One way to prolong the life of monocytes is by inhibiting apoptosis by granulocyte-colony-stimulating factor (GM-CSF), which is a hematopoietic-stimulator that enhances the survival, proliferation and rate of differentiation of hematopoietic cells. In my presentation, therapeutic effects of GM-CSF in limb ischemia by gene therapy will be presented and discussed. A Hematopoietic Stem Cell (HSC) Specific microRNA Gives Novel Insights into the Regulation of HSC Homeostasis and Allows Safer HSC-based Gene Therapy 1,2 1,2 1 3 3 3 Bernhard Gentner , Alice Giustacchini , Ilaria Visigalli , Eric Lechman , Peter van Galen , Hidefumi Hiramatsu , Francesco 1,2 1 1,2 3 1 1,2 Boccalatte , Massimo Saini , Silvia Ungari , John E Dick , Alessandra Biffi and Luigi Naldini . 1 2 San Raffaele Telethon Institute for Gene Therapy; Vita-Salute San Raffaele University, San Raffaele Scientific Institute, 3 Milan, Italy; Division of Stem Cell and Developmental Biology, University of Toronto, Toronto, Ontario We report that miR-126, a microRNA preferentially expressed in Hematopoietic Stem Cells (HSC) among the hematopoietic lineage, plays a pivotal role in restraining cell cycle progression of HSC in vitro and in vivo. miR-126 knockdown expanded functional mouse and human HSC in vivo, without HSC exhaustion. Conversely, enforced miR-126 expression increased the fraction of phenotypic HSC in G0. miR-126 control of proliferation is attributable, in part, to attenuation of signal transduction by the PI3K/AKT/GSK3β axis. We propose that miR-126 establishes a threshold for HSC activation and provides negative feedback aiding the return of stimulated HSC to quiescence. Our results establish that the HSC quiescence/activation equilibrium is regulated by miRNAs in a mechanism conserved between mouse and human. The discovery of HSC-specific microRNAs also prompted us to design novel vectors for HSC-mediated gene therapy with enhanced efficacy and safety. By adding miR-126 target sequences (miRT) to a vector cassette, miR-126 activity was harnessed to negatively regulate transgene expression in HSC and target expression to differentiated hematopoietic cells. These HSC-off vectors allow delivering a transgene into HSC without affecting its proteome, while achieving sustained multi-lineage expression in the progeny. The utility of this new vector was demonstrated for the gene therapy of Krabbe disease in the mouse model. miR-126 regulated vectors overcame hematopoietic toxicity associated to unregulated galactocerebrosidase (GALC) expression in HSC, while delivering substantial amounts of GALC enzyme in the nervous system with improved survival of the affected mice. Conditional models for Chimeric Antigen Receptor (CAR) based activation of T lymphocytes Martin Bonamino Instituto Nacional de Cancer – Rio de Janeiro – Brazil The modification of T lymphocytes with Chimeric Antigen Receptors (CARs) represents a promising strategy for combined adoptive T cell based immune-gene therapy of cancer. Current CAR molecules are constructed fusing an immunoglobulinderived Fab-based antigen recognition domain in the scFv configuration, a transmembrane domain and signaling domains promoting T cell activation. Clinical trials based on this strategy have reported impressive hematological responses for leukemia and lymphomas for CARs designed against CD19, CD20 or CD30 antigens. One potential limitation of this strategy is the off-target effects observed when target antigens are expressed in healthy tissues. To circumvent this limitation we are considering conditional activation based on two CARs in a way that only the correct antigen combination induces complete T cell activation. This strategy has the potential to expand the panel of CAR-targeted antigens, narrowing T cell responses based on CAR mediated antigen recognition and increasing the safety of CAR-based immune-gene therapies. 55 Symp#4 Cell Biology & Reproduction Chair Luiz Renato França Spermatogonial Stem Cell Niche in Vertebrates Luiz R França Luiz Renato França,Paulo HA Campos Júnior, Guilherme MJ Costa, Samyra SMSN Lacerda, Gleide F Avelar, Alana L Sousa, *Marie-Claude Hofmann Laboratory of Cellular Biology, Dept. of Morphology, ICB/UFMG, Belo Horizonte, MG, Brazil. *MD Anderson Cancer Center, Dept. of Endocrine Neoplasia and Hormonal Disorders, Houston, TX, USA (email: [email protected]) Spermatogonial stem cells (SSCs) are located in a particular environment called the “niche” that is controlled by the basement membrane, key testis somatic cells, and factors originating from the vascular network. Although crucial for SSC physiology, the niche is still poorly understood, particularly in non-model vertebrates where the testis cytoarchitecture + could provide important cues for niche components and regulation. Recently, we demonstrated that Aund GFRA1 cells present preferential location (nearby blood vessels) in vertebrate species other than mouse and rat, such as zebrafish, bullfrog, turtle and horse. Additionally, we observed that peccaries present a peculiar Leydig cell (LC) distribution, whereby these cells situate around lobes of seminiferous tubules. Since the role of LCs as a niche component is not yet clearly elucidated, this feature makes the peccary an interesting model for investigating the SSC niche. Subsequently, we + observed that in peccaries, ~93% of Aundspermatogonia are GFRA1 and that these cells are preferentially located adjacent to the interstitium without LCs. Moreover, the expression of CSF-1 was observed in LCs and peritubular myoid cells (PMCs) + while its receptor was present in LCs and in GFRA1 Aund. In summary, besides reinforcing the fundamental role of Sertoli cells in GDNF-GFRA1 signaling for SSC self-renewal in vertebrates, our data suggest that the mechanisms involved in SSC physiology may be conserved in vertebrates. However, our peccary findings indicate that, contrary to PMCs, LCs might play a minor role in the SSC niche/physiology and that LCs are probably involved in the differentiation of Aund toward type A1 spermatogonia. Fetal Testis Differentiation and Function, its Regulation and its Disorders Richard M Sharpe, Rod Mitchell, Afshan Dean, Karen Kilcoyne, Sophie Platts, Ashley Boyle, Sheila Macpherson, Chris McKinnell, Richard Anderson, Sander van den Driesche MRC Centre for Reproductive Health, The Queen’s Medical Research Institute, University of Edinburgh, UK (contact: [email protected]) Differentiation of the testis represents the first step along the pathway to becoming a male. Understanding of the processes that regulate testis differentiation have added importance because there is increasing evidence that the commonest disorders of human male reproductive health may largely stem form this period in life. Thus the testicular dysgenesis syndrome (TDS) hypothesis proposes that subnormal ‘set-up’ of the fetal testis/cell types leads to subnormal function (especially of the fetal Leydig cells) which, in turn, leads to male reproductive disorders that manifest at birth (cryptorchidism, hypospadias, micropenis) or in adulthood (low sperm count, low-normal testosterone, testis germ cell cancer). Direct evaluation of this hypothesis in humans is difficult, so we have used a rat model of TDS (fetal exposure to dibutyl phthalate; DBP) to help elucidate key mechanisms and cell-cell relationships in the fetal testis, disruption of which leads to TDS disorders. These have helped identify the masculinisation programming window (MPW) within which testosterone production by fetal Leydig cells is critical for determining normality and ultimate adult size of all male reproductive organs; deficiency in testosterone production in the MPW determines risk of later TDS disorders and can be ‘measured’ retrospectively by anogenital distance (AGD). Our studies show that DBP-induced focal dysgenesis (malformation of seminiferous cords, intratubular Leydig cells, mis-specification of somatic cells) is closely interlinked with deficiency in testosterone production in the MPW, even though the dysgenesis manifests after the MPW. The mechanisms and factors involved, insofar as they are understood, will be discussed. Antimicrobial Proteins Secreted by the Epididymis Maria Christina W. Avellar Section of Experimental Endocrinology, Department of Pharmacology, Universidade Federal de São Paulo – Escola Paulista de Medicina (UNIFESP-EPM), São Paulo, SP, 04044-020, Brazil. This presentation will focus on the expression and regulation of naturally occurring antimicrobial proteins secreted by the epididymis. Aspects of the pattern of expression of mRNA and protein for selected genes, highlighting isoforms expressed by the beta-defensin SPAG11B gene, in the adult tissue and during development of the rat epididymis will be discussed. The effects of androgens, luminal fluid, glucocorticoids and exposure to in vivo bacterial products on their expression and immunolocalization will be also presented. Financial Support: FAPESP, CNPq, CAPES and Fogarty International Center (subcontract UNIFESP-EPM/University of North Carolina at Chapel Hill, USA). Email: [email protected]. 56 Symp#5 Host Parasite Interaction Chair Wanderley de Souza Wanderley de Souza Federal University of Rio de Janeiro (UFRJ), Rio de Janeiro, Brazil Introductory Notes Dephosphorylation of eIF5A is Required for Translation Arrest at the Stationary Growth Phase of Trypanosoma cruzi Chung, J.*; Rocha, A. A.*, Tonelli, R. R.;,Castilho, B. A., and Sérgio Schenkman. Departamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal de São Paulo, São Paulo, Brazil * Both authors contributed equally The protein known as eukaryotic initiation factor 5A (eIF5A) has an elusive role in the translation elongation. It has a unique and essential hypusine modification on a conserved lysine residue in most eukaryotes. In addition, this protein is modified by phosphorylations with unknown functions. Here we found that a phosphorylated state of eIF5A from Trypanosoma cruzi (TceIF5A), the protozoan that causes Chagas’ disease, predominates in exponentially growing cells and extensive dephosphorylation occurs in cells reaching the stationary growth phase. The phosphorylation was shown to occur mainly at Ser 2, then at Ser 47, homologous to yeast eIF5A. In addition, a novel phosphorylation site was identified at Tyr 21. In exponential cells, TceIF5A is partially associated with polysomes, compatible with its function as an elongation factor and become relatively enriched in polysomal fractions at stationary at stationary phase. TceIF5A overexpression increases the rate of cell proliferation, protein synthesis, and the relative amount of polysomes. When Ser 2 is replaced by Asp, but not by Ala, in the overexpressors, the cells still show an increased protein synthesis and growth rate. However, the presence of Asp causes cell damage when cultures reach the stationary phase. Wild type, or Ala, but not Asp replacing Ser 2 forms of TceIF5A causes protein synthesis arrest and are enriched in polysomal content at the stationary phase. We conclude that dephosphorylation of eIF5A has a key role in arresting the protein synthesis under unfavorable conditions, indicating that eIF5A phosphorylation/dephosphorylation might control its association with polysomes during translation elongation. Ion Regulation in the Malaria Parasite: The Target of a New Generation of Antimalarials Kiaran Kirk Research School of Biology, The Australian National University, Canberra, ACT, 0200, Australia The intraerythrocytic malaria parasite induces novel channels in the membrane of its host erythrocyte. These channels mediate the uptake of essential nutrients but, at the same time, allow a net influx of Na+ ions, resulting in an elevated Na+ concentration in the host cell compartment. The intraerythrocytic malaria parasite itself maintains an intracellular Na+ concentration some ten-fold less than that in its host cell, extruding Na+ via a Na+-ATPase on its plasma membrane. Bioinformatic analysis of the genome of the human malaria parasite Plasmodium falciparum reveals that the most likely candidate for the parasite’s Na+ ATPase is a protein known as PfATP4. The spiroindolones are a new class of compound that inhibit the in vitro growth of the human malaria parasite, Plasmodium falciparum, at nanomolar concentrations. One of the spiroindolones is now in Phase IIa clinical trials, and is the first molecule with a novel mechanism of action to enter Phase IIa studies for malaria in the last 20 years. Prolonged exposure of P. falciparum parasites to sub-lethal concentrations of spiroindolones leads to the emergence of spiroindolone-resistant parasites, with the resistance attributable to mutations in PfATP4. In this talk I will introduce the cell physiology of the intracellular malaria parasite and present evidence that the spiroindolones exert their antimalarial effect by inhibiting Na+ extrusion via PfATP4, thereby disrupting Na+ regulation in the intracellular parasite. Why coinfect cells with non-viral pathogens? Michel Rabinovitch Universidade Federal de São Paulo – Escola Paulista de Medicina, Departamento de Microbiologia, Imunologia e Parasitologia, São Paulo, Brazil e-mail: [email protected] Coinfection of E. coli bacteria with mutant bacteriophages in the 1940s spurred the development of molecular virology. Beginning in the 1960s, virologists, among them alumni of the CSHL Phage Course, coinfected mammalian cells with virus mutants, strains or species and developed the basic procedures and concepts of viral genetics. The AIDS pandemic of the 1980s begot cell coinfections of HIV and non-viral pathogens. In the 1990s, impressive horizontal gene exchanges were detected between pathogens sheltered in free-living protozoa. In contrast, there are few reports–briefly reviewed in the presentation-of mammalian cells coinfected with bacteria or protozoa. Initially, the model permitted the targeting of pathogens to intracellular compartments they normally don’t occupy, thanks to C. burnetii’s exceptionally large and hospitable parasitophorous vacuoles. Vacuolar colocalization will probably be rare in the world of coinfections. We believe that coinfection, apart from its ludic quality, may uncover unexpected, and hopefully interesting, interactions involving (for instance), antagonism via secreted toxins or antibiotics, competition for cell derived nutrients, quorum sensing effects, exploitable modulation of host cell gene expression and transduction cascades. We thus argue that coinfection with non-viral-pathogens could provide an added tool for mono-infection-entrenched cellular microbiologists. However, whereas mono-infections may reflect a dialogue – by proxy - between two genomes, the neo-coinfector may be confronted with a livelier dialogue involving three – genomes, besides the potential participation of a fourth, the host cell mitochondrial genome. One problem remains: given the number of available microorganisms, how is one to select the most promising pair for coinfection? 57 Symp#6 Membrane Biology Chair José Garcia Abreu New Inhibitory Wnt/β-catenin Mechanisms Affecting Embryonic Head Formation Jose Garcia Abreu, PhD. Instituto de Ciencias Biomédicas – Universidade Federal do Rio Janeiro. Rio de Janeiro – Brazil. The establishment of vertebrate embryonic axes involves a series of cellular and molecular events right after fertilization. In the frog embryo the dorsal ventral axis relies on accumulation of dorsal beta-catenin which together with Nieuwkoop center set the dorsal organizer, also known as Spemann Organizer. Upon the onset of gastrulation a number of secreted factors act dorsally preventing dorsal fate from ventral signals. At the same time cells from the prechordal plate endomesoderm secrete inhibitors of ventro-lateral Wnt signals setting up the antero-posterior axis. Therefore, Wnt/beta-catenin signaling plays major role in the establishment and patterning of embryonic axis. Here, we present a new Wnt-beta-catenin inhibitor, expressed in the Spemann Organizer which is essential for proper head formation. We also present data on how cholestero-rich membrane microdomains interfere with the morphogentic fields in the head organizer, the pre-chordal plate. Support: FAPERJ, CNPQ and CAPES. Membrane Dynamics and Mechanics of Signaling: Role of Caveolae Sinha, B., D. Koster, R. Ruez, P. Gonnord, M. Bastiani, D. Abankwa, R.V. Stan, G. Butler-Browne, B. Vedie, L. Johannes, N. Morone, R.G. Parton, G. Raposo, P. Sens, C. Lamaze, and P. Nassoy. 2011. Cells respond to mechanical stress by rapid disassembly of caveolae. Cell. 144:402-13. Nassoy, P., and Christophe Lamaze. Stressing caveolae new role in cell mechanics. Trends Cell Biol. in press Institut Curie, Paris, France The functions of caveolae, the characteristic plasma membrane invaginations, has long remained debated. The particular abundance of caveolae in endothelial and muscle cells cells, which respectively experience shear stress and stretching, led us to investigate the role of caveolae in membrane-mediated mechanical response. Acute mechanical stress induced by osmotic swelling or by uniaxial stretching results in a rapid disappearance of caveolae, in a reduced caveolin/Cavin1 interaction, and in an increase of free caveolins at the plasma membrane. Tether-pulling force measurements in cells and in plasma membrane spheres demonstrate that caveola flattening and disassembly is the primary actin- and ATP-independent cell response that buffers membrane tension surges during mechanical stress. Conversely, stress release leads to complete caveola reassembly in an actin- and ATP-dependent process. We further show that mechanosensing through caveolae in endothelial cells involves the Jak/Stat signaling pathway. Caveolae are therefore mechanosensors and mechanotransducers which constitute a physiological membrane reservoir that quickly accommodates sudden and acute mechanical stresses. Studying Spatial Control of Exocytosis at the Nanoscale in Living Cells Derek Toomre Yale University School of Medicine, USA Cells spatially control exocytosis to control a range of biological function – from cell division, migration, invasion and the formation of specialized structures such as the primary cilium. Loss of this spatial-temporal control can lead to diseases including cancer, diabetes, and polycystic kidney disease. This seminar will focus firstly on new superresolution ‘nanoscopes’ and their power to visualize subcellular processes with incredible detail. I will then show how it to query and even optogenetically manipulate the very last steps of exocystosis, vesicle tethering and fusion. As case studies, I will show how this approach has given new insight and lead to new concept in cytokinesis, cell migration, and insulin-mediated trafficking of Glut4 in adipocytes. For examples, in adipocytes we see two membrane trafficking pathways to the surface and a regulation by insulin on the expansion of the fusion pore. Challenges and potentials of these new nanoscopes, with special emphasis to membrane traffic, will be discussed. 58 Symp#7 Signaling in development Chair Ricardo Guelerman Pinheiro Ramos Ricardo Guelerman Pinheiro Ramos University of São Paulo, Ribeirão Preto, Brazil Introductory notes Wnt Signaling, Stem Cells and Tissue Repair Roel Nusse, Howard Hughes Medical Institute, Department of Developmental Biology, Lorry I. Lokey Stem Cell Research Building. Stanford Work from many laboratories has shown that Wnt signals are essential for the control over stem cells. A right balance between the number of stem and differentiated cells is essential for the proper function of organs. Locally acting signals, including Wnts, are important to maintain this balance in a spatially organized manner and these signals are key to understanding the regulation of growth. How this is achieved is far from clear and is the subject of studies in our lab, both in vivo and in cell culture. In vivo, a particular question we address is how physiological changes, such as those occurring during hormonal stimuli, injury or programmed tissue degeneration have an impact on the selfrenewal signals and on stem cell biology. Current research includes: 1) Identifying and tracing Wnt-responsive stem cells in tissues; 2) mapping cis-acting transcriptional control elements (enhancers) that control Wnt gene expression in normal and injured tissues; 3) the use of active Wnt proteins to maintain stem cells (including embryonic stem cells) in cell culture; 4) presenting Wnt protein in a vectorial manner to stem cells to direct asymmetric cell division. Formation of the Vertebrate Body Axis Olivier Pourquié Institut de genétique et biologie moleculaire et celulaire, INSERM, France Hedgehog Signaling in Development and Disease Matthew Scott Departments of Developmental Biology, Genetics, and Bioengineering, Howard Hughes Medical Institute, Clark Center West Wing W252, 318 Campus Drive, Stanford University School of Medicine, Stanford, California 94305-5439 Phone 650-725-7680 Fax (650) 725-2952 Hedgehog (Hh) signaling is important for the development of most organs and tissues. Damage to Hh signal transduction components causes birth defects and cancer. We have been exploring four areas of Hedgehog signaling: transduction in primary cilia, roles of Neuropilin proteins, identification of direct Hh target genes in the cerebellum and medulloblastomas (MBs), and mutations in MB tumor genomes. Neuropilins 1 and 2 (Nrp1, 2) are transmembrane proteins with roles in axon guidance and vascular endothelial growth factor (VEGF) signaling. We found that they are important positive regulators of Hh signal transduction. Nrps are expressed at times and locations of active Hh signal transduction during mouse development. We show that Nrps mediate Hh transduction between activated Smoothened (Smo) protein and the negative regulator Suppressor of Fused (SuFu). Nrp1 transcription is induced by Hh signaling and Nrp1 over-expression increases maximal Hh target gene activation, indicating the existence of a positive feedback circuit. We are testing the importance of Nrps for growth of MB cells. Nrps act upstream of Gli proteins, transcription factors that directly control Hh target gene transcription. We have identified direct targets of Gli1 in normal mouse cerebellum development and in MBs, and found that Gli1 is located at some common genes in the two cell types as well as many locations unique to each cell type. To understand better the properties of MB tumor cells we are determining exome sequences of human and mouse tumors. 59 Symp#8 Epithelial proliferation and differentiation Chair Mari Sogayar Mari Sogayar Department of Biochemistry, Chemistry Institute, University of São Paulo, São Paulo, Brazil Introductory notes and talk Intrinsic And Extrinsic Regulation Of Epidermal Stem Cell Fate Fiona M Watt CRUK Cambridge Research Institute, Li Ka Shing Centre, Robinson Way, Cambridge CB2 0RE, UK When an epidermal stem cell divides its progeny can either remain stem cells or undergo terminal differentiation. These cell fate decisions are controlled both by intrinsic mechanisms and by external signals from the local microenvironment or niche. Interactions between epidermal stem cells and the niche are reciprocal, since stem cells are capable of remodelling their environment. My lab is investigating the interplay between specific intrinsic and extrinsic signals in regulating stem cell fate in adult epidermis. We find that both in vitro and in vivo approaches are informative and conclude that the way that a stem cell behaves is to a large extent determined by extrinsic signals. Wellcome Trust Centre for Stem Cell Research , University of Cambridge (CSCR), and the other in Cancer Research UK Cambridge Research Institute (CRI) Differential roles for NFAT transcription factor isoforms in cell transformation João Viola Program of Cellular Biology, Instituto Nacional de Câncer (INCA), Rio de Janeiro, RJ, Brazil. The nuclear factor of activated T cells (NFAT) family of transcription factors are inducible proteins that play a key role in gene expression. The NFAT family is composed of four calcium-responsive proteins (NFAT1-4). Each NFAT gene may be alternatively spliced into two or more isoforms that differ at the N- and/or C-termini, although the core of the DBD and NHR regions remain conserved. Once NFAT is activated, these proteins can bind to their target promoter elements and activate the transcription of specific responsive genes, either alone or in combination with other nuclear partners. Regardless of their widely known cytokine gene expression properties, NFAT transcription factors have been shown to regulate other genes related to cell cycle progression, cell differentiation and apoptosis, unraveling a broader role for these proteins in normal cell physiology. Recent studies suggest that the NFAT family of transcription factors plays a much broader role in cell proliferation and apoptosis, and their contributions to tumorigenesis are becoming clearer. Here, we demonstrate that three of NFAT proteins (NFAT1 and NFAT2 - and -isoforms) induce distinct phenotypes in NIH3T3 cells and the differential roles for NFAT family members are partially mapped to the transactivation domains (TAD) located at the N- and C-terminal end of these proteins. In fact, our results suggest that NFAT1 and NFAT2 isoform act as tumor suppressor genes, mainly by inducing cell death and cell cycle arrest, whereas NFAT2 -isoform act as an oncogene, by protecting cells from apoptosis. Finally, our results support distinct roles for the different isoforms of NFAT transcription factors in cell transformation. Financial Support: ICGEB, INCT-Cancer, FAPERJ, CNPq and CAPES. Contact: [email protected] 60 Symp#9 Immune Cell Biology Chair Wilson Savino Wilson Savino Instituto Oswaldo Cruz, FIOCRUZ, Rio de Janeiro, Brazil Introductory notes and talk Immunological and Clinical Outcomes of a New DC-Based Vaccine Flavio Salazar-Onfray and Mercedes López 1 Millennium Nucleus on Immunology and Immunotherapy, Faculty of Medicine, University of Chile. We developed an original method for production of therapeutic dendritic-like cells named Tumor Antigen Presenting ® Cells (TAPCells ) using an allogeneic melanoma-derived cell lysate (TRIMEL) as activation factor and antigen provider. TAPCells-based immunotherapy induced T cell-mediated immune responses and improved long-term survival of stage IV patients in studies involving more than 100 individuals (López et al. 2009, J Clin Oncol; Aguilera et al. 2011, Clin Cancer Res). Importantly, 61% of tested patients (58 out of 94) showed a Delayed Type Hypersensitivity (DTH) reaction against TRIMEL indicating the development of anti-tumor immunological memory that correlates with prolonged patient survival. The in vitro analysis of TRIMEL showed that it contains damage associated molecular patterns such as HMBG-1 protein, induced by heat shock, capable to improve, through TLR4, the DC maturation and antigen crosspresentation. In fact, a TLR4 polymorphism correlates with patient clinical outcome. DTH response against TRIMEL was associated with prolonged survival of the stage IV responder melanoma patients (DTH +; 35 months) compared to the non-responders (DTH -; 11 months). Furthermore, we observed that DC-vaccination resulted in a three-fold augment of Th1 cell population releasing IFN-γ and a two-fold increase of Th17 lymphocyte population capable to produce IL-17 in the PBL of DTH+ patients respect to DTH- ones. Antibodies against melanoma antigens can be detected in the sera from several vaccinated patients, althougth no correlation with clinical responses could be established. Taken together, our results indicate that TAPCells immunization resulted in two different pattern of response associated to the immunological and clinical outcome. Our study may contribute to the better understanding of clinical immunological responses produced by DC-vaccines and to the development of improved DC-based vaccines. Supported by Fondecyt 1090238 and 1090243. Eph/ephrin-mediated Interactions Govern Functional Maturation of Developing Thymocytes in the Thymic Epitelial 3D Network 1 2 1 1 2 1 Agustín G Zapata , Juan J Muñoz , David Alfaro , Javier Gª Ceca , Teresa Cejalvo , Esther Trobajas , Sara Montero (1) Department of Cell Biology, Faculty of Biology, (2) Centre for Cytometry and Fluorescence Microscopy, Complutense University, 28040 Madrid, Spain 1 The thymus is a primary lymphoid organ in which a 3D epithelial network supports the functional maturation of lymphoid progenitors into T lymphocytes. The process is highly dependent on the migration of developing thymocytes to the adequate thymic niche in which thymic epithelial cell (TEC)-thymocyte interactions are critical. In the current presentation we report the role played in these processes by Eph and ephrins, a large family of receptors and ligands, respectively involved in the organogenenesis and homeostasis of numerous tisssues, regulating cellular attachment/detachment. They are extensively expressed in the thymus, partially govern colonization of lymphoid progenitors and their migration throughout thymic parenchyma and their lack deeply affects not only T-cell maturation but also correct organization of thymic epithelial network, reflecting the relevance of these molecules in the thymocyte-TEC interactions that largely modulate the biology of thymus 61 Symp#10 Cell Biology and Education ASCB & IFCB Symposium Chairs Bruce Alberts and Cynthia Jensen Cell Biology and Education Jensen, C.G. Department of Anatomy with Radiology University of Auckland, Auckland, New Zealand Although Cell Biologists have a wide range of research interests, they all have a common interest in teaching cell and molecular biology to undergraduate and postgraduate students and in training masters and PhD students and postdoctoral fellows in research techniques. The speakers and discussants at this symposium will describe a variety of methods of teaching and training in cell biology, including descriptions of special courses and distance teaching. There will be an opportunity for discussion, and questions and comments from the audience will be encouraged. Teaching at Distance: Interactive Multimedia of the Cell Biology of Trypanosoma cruzi 1, 2 2 1,4 3, 4 Benchimol, M. , Teixeira, D.E. , Crepaldi, P.H. ; De Souza, W. 1 Universidade Santa Úrsula, Rio de Janeiro, RJ, Brasil 2 Fundação CECIERJ, Rio de Janeiro, RJ, Brasil. 3 Instituto de Biofisica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Brasil 4 INMETRO, Rio de Janeiro, RJ, Brasil CECIERJ is a state public foundation focused in education. It includes the CEDERJ arm, which is specialized in teaching at distance as university. Our group has developed an intense work producing multimedia material to undergraduate students in Biology. The aim of this work was to develop educational materials in the graphical version using threedimensional (3D) animations, to visualize the morphology, dynamic processes and basic knowledge of the Cell Biology as a whole and here, in special of Trypanosoma cruzi, the causative agent of Chagas´ disease. Parasitic protozoa are important agents of human and veterinary diseases not only in Brazil but also all over the world. The life cycle of these protozoa is presented in different levels of education, from fundamental school to graduation level. Videos and animations include: cell division, endocytosis and flagellar beating; the interaction of the parasite with a vertebrate host cell and the behavior of this protozoan in the digestive tract of the invertebrate host. Thus, this material could: (1) facilitate the cell biology of parasites learning and teaching, (2) provide good material which can be used by several people at different levels, such as lectures, classes, research, thesis, etc. Supported by CNPq, FAPERJ and CECIERJ Engaging Undergraduate Students in Research Kiaran Kirk Research School of Biology, The Australian National University, Canberra, ACT, 0200, Australia There are many benefits to be gained from engaging high-achieving students in ‘real research’ from as early as possible in their undergraduate career. At the Australian National University we offer students the opportunity to do laboratory-based ‘Biology Research Projects’ that count as courses towards their Science degree. We have also introduced a research-focused (and highly-selected) degree, the ‘Bachelor of Philosophy’, in which a quarter of the courses taken over a three year period are in the form of research projects. Our experience with this mode of teaching will be discussed. 62 Symp#11 Glia Club Chairs Bernardo Castellano and Vivaldo Moura Neto GSK3β is a Profound Negative Regulator of Oligodendrocyte Differentiation and Myelination Arthur M. Butt, Andrea Rivera and Kasum Azim Institute of Biomedical and Biomolecular Sciences, University of Portsmouth, U.K. Oligodendrocytes are the myelinating cells of the central nervous system and are the primary targets of tissue destruction in the demyelinating disease multiple sclerosis. The enzyme GSK3β is a target of many receptor-mediated signalling pathways that regulate the differentiation of from their precursors (OPCs). We have examined this using a range of small molecular inhibitors of GSK3β in the mouse brain. Inhibition of GSK3β stimulates the generation of OPCs from neural precursor cells (NPCs) in the subventricular zone (SVZ), involving the canonical Wnt-β-catenin signalling pathway. Significantly, inhibition of GSK3β also dramatically stimulates oligodendrocyte differentiation and myelination. A key finding is that GSK3β inhibition has equivalent effects in the adult and stimulates the regeneration of oligodendrocytes and remyelination following demyelination in the adult forebrain. Using a genome wide microarray approach, we find that GSK3β inhibition regulates oligodendrocyte generation via multiple positive and negative regulatory signalling pathways. GSK3β inhibition significantly upregulated Sox10 and Olig2, key positive regulators of oligodendrocyte differentiation, and down-regulated the Wnt and Notch signalling pathways, together with helix-loop-helix ID (inhibitor of differentiation), which are dominant negative regulators of oligodendrocyte differentiation. This study identifies novel functions for GSK3β as a profound negative regulator of oligodendrocytes at all stages of their differentiation in vivo. Moreover, our findings indicated that GSK3β signalling pathways contributed to inefficient regeneration of oligodendrocytes and myelin repair in demyelination. Role of Cancer Stem Cells in Adult and Paediatric Brain Neoplasms: Hoax or Holy Grail? Professor Geoffrey J Pilkington BSc PhD CBiol FSB FRCPath Professor of Cellular and Molecular Neuro-oncology, University of Portsmouth, St Michael’s Building, White Swan Road Portsmouth PO1 2DT UK In the 1970s stem cell-like populations were described in ethylnitrosourea-induced rat glioma and hypothesised as the origin of such tumours. These were located around blood vessels and degenerating neurones, sub-ependymally, at the lateral ventricles. More recently cancer stem cells (CSCs), reported largely on their expression of CD133, gathered considerable interest in adult and paediatric brain tumours. Initial observations that the CD133+ population were the initiators of brain tumours, based upon their ability to produce tumours in xenograft models while CD133- failed to do so have, however, been challenged. The value of CD133 (Prominin-1) has therefore been questioned but the function of CD133 or its possible link with processes underlying tumour development and progression remains obscure. Adult glioblastoma biopsies yield only 1-5% CSCs based upon CD133 immunostaining. We described a low passage paediatric glioblastoma culture where over 40% of the cells express CD133 and, if transferred to neural basal medium under hypoxic conditions, this elevated to >95% CD133+. Magnetic bead immunoseparated CD133+ and CD133- were used for genetic profiling, adhesion, invasion, and proliferation assays in response to nuclear- and mitochondrially-acting pro-apoptotic agents. We also investigated the significance of CD133 glycosylation and influence of oxygen on such glycosylation. Whether or not CD133 is a marker of CSCs in brain tumours remains controversial but it may be of significance to the biology of paediatric brain neoplasms and requires further investigation both in vitro and in tissue sections. Hoax? certainly not, but CD133 must be put into context and, while the Holy Grail issue remains unresolved, this is an area of intense interest in unravelling the mysteries of tumour biology. Mesenchymal Stem Cells lower proliferation and invasion of Glioblastoma cells, exploiting the Immune Response Mediating Chemokines Tamara T. Lah, Motaln H1 , Gruden K2, Hren M2, Primon M1 and Schichor Ch3 1. Department of Genetic Toxicology and Cancer Biology, National Institute of Biology, Ljubljana, Slovenia. 2 Department of Biotechnology and Systems Biology, National Institute of Biology, Ljubljana, Slovenia. 3 Klinikum Großhadern, Ludwig-Maximilians-Universität, München, Germany. Human mesenchymal stem cells (hMSC) are gaining the forefront position in therapies for curing several diseases. The majority of them is focused on the improvements in the regenerative medicine, whereas only limited number of studies is addressing the potential use of hMSC for anti-cancer therapy, although the findings in several different cancer models are suggesting they could be developed into efficient cellbased therapeutics. Considering recent in vivo and in vitro reports on hMSCs growth inhibitory effect on most malignant brain tumor – glioblastoma multiforme (GBM) we focused here on the cellular processes responsible for this inhibitory effect, such as cell proliferation, invasion and senescence which we confirmed in several GBM lines. We performed whole genome mRNA analysis and cytokine profiling of both, the hMSC and the U87MG GBM cells grown in co-cultures. We found that several chemokines may account either for the decrease of both, proliferation and invasion of U87-MG, as well as for induced MSCs proliferation and invasion when the two cells were grown in the indirect co-cultures. CCL2/MCP-1 was collectively identified as one of the few most significantly up-regulated chemokine responsible for hMSC and U87-MG paracrine signaling and we functionally confirmed its role in GBM cell invasion in vitro. In conclusion, our results indicate the CCL2/MCP1 to be the key player of paracrine MSC/glioma cell interactions. Several other gene/protein putative markers were identified to take part in hMSC/glioma cell communication for the first time. Together with CCL2/MCP-1 those markers could be utilized in future, as target genes for cell-based anticancer therapy. Effects of cns-targeted il-6 or il-10 production on microglial activation and motor neuron degeneration after facial nerve axotomy B. Castellano1, N. Villacampa1, B. Almolda1, I.L. Campbell2 and B. González1 1Department of Cell Biology, Physiology and Immunology, Institute of Neurosciences. Universitat Autònoma de Barcelona, Spain. 2School of Molecular Bioscience, University of Sydney, Sydney, NSW 2006, Australia Interleukin-6 (IL-6) and Interleukin-10 (IL-10) are key cytokines with an important role in the regulation of the inflammatory and immune responses. In the central nervous system (CNS), increased expression of both IL-6 and IL-10 occurs in a wide range of pathological conditions. Meanwhile IL-6 has been usually recognized as a cytokine with a dual role acting as a pro-inflammatory or anti-inflammatory signal inducing glial activation while IL-10 is mainly involved counteracting the inflammatory response and immune reactions. The objective of the present study was to evaluate the effects of local production of either IL-6 or IL-10 in the microglial response, lymphocyte infiltration and neuronal degeneration induced by transection of the facial nerve, a sterile neuronal injury model. Facial nerve axotomy (FNA) was performed in transgenic mice with astrocyte-targeted production of either IL-6 (GFAP-IL6Tg) or IL-10 (GFAP-IL10Tg) and their corresponding wild-type (WT) littermates. The analysis was performed by histology, immunohistochemistry and flow cytometry at different time points, ranging from 3 to 42 days post-lesion. Our observations clearly indicate that GFAP targeted expression of either IL-6 or IL-10 exerts a direct impact on the pattern of microglial activation and neuronal degeneration/survival or axotomized motor neurons. As will be discussed, changes observed in the expression of different molecules such as Iba1, CD11b, CD16/32, MHC class II and some integrins like osteopontin and their receptors (CD44 and 5) may be involved in the differential glial activation pattern and lymphocyte recruitment producing a specific outcome of facial nerve axotomy. Supportedby Ministerio de Ciencia e Innovación (BFU2008-04407/BFI) (BFU2011-27400) and NH&MRC grant 632754 63 Symp#12 Protein Folding and Assembly Chair Carlos Ramos Protein Folding and Assembly Carlos Ramos Chemistry Institute, UNICAMP, Campinas, Brazil Protein folding and assembly have strong biotechnological and medical relevance, and understanding how proteins fold into their native structures has long been a major goal for researchers aiming to predict structure from the primary amino acid sequence. Protein homeostasis is relevant for several cellular processes, such as aging, neurodegenerative diseases, evolutionary processes, and synaptic plasticity. Although failure to reach or maintain the correct folded structure leads to serious consequences, under normal circumstances aberrant proteins become eliminated. A correct balance between folding and the degradation of misfolded proteins is maintained by a basic cellular phenomenon known as protein quality control (PQC). This correct balance is critical for cell viability, particularly when considering macromolecular crowding in the intracellular environment. In this environment, improper associations between partially unfolded proteins are enhanced, and mechanisms that prevent incorrect folding or help to eliminate irreversible aggregates that may be harmful to cells are necessary. The resulting misfolded proteins may be degraded by proteases or repaired by chaperones, but proteins that escape PQC will probably aggregate. In this symposium speakers will discuss recent advances in our knowledge of how proteins fold and assembly inside the cell. Mechanisms for folding corrector action in rescue of mutant CFTR from premature degradation by ER Quality Control Douglas M. Cyr Department of Cell Biology, School of Medicine, UNC-Chapel Hill, Chapel Hill, NC, 27599 CF patients inherit a variety of mutations that cause folding defects in CFTR, which lead to its recognition for premature degradation by Hsp70 dependent E3 ubiquitin ligases (RMA1 and CHIP) on the cytoplasmic face of the ER. The folding defects in ΔF508-CFTR, as well as defects in other rare mutants, are correctable, but the mechanism of action for folding correctors is unknown. ΔF508 occurs in nucleotide binding domain 1 (NBD1) and blocks Cl- channel assembly by hindering interactions of NBD1 with intracellular loops exposed by MSD1 and MSD2. Therefore, correction of folding defects in ΔF508-CFTR requires assembly to a conformation to permits escape form multiple ERQC machines and may require repair of more than one folding defect. This talk we describe current knowledge about the mechanism for recognition of misfolded CFTR by ERQC machines and present information on the mechanism for folding corrector action in treatment of CF. Prospects for treatment ΔF508 homozytotes and compound heterozygotes with different combinations of folding correctors will be discussed. This work is supported by grants from the National Institutes of Health and the North American Cystic Fibrosis Foundation. Chaperonopathies: Impact on protein folding and beyond Alberto J. L. Macario, a,b and Everly Conway de Macarioa a Department of Microbiology and Immunology, School of Medicine, University of Maryland at Baltimore, and IMET, Baltimore, MD, USA; bIstituto Euro-Mediterraneo di Scienza et Tecnologia (IEMEST), Palermo, Italy. Chaperonology encompasses the study of molecular chaperones and heat-shock proteins in all their aspects, normal and abnormal; physiological and pathological, including medico-clinical; biochemical; molecular biological; genetic; and biological. A subfield of Chaperonology deals with the chaperonopathies, i.e., diseases in which chaperones play a pathogenic role, participating in the mechanism of disease as etiologic-pathogenic factors. These diseases can be classified as any other in the Medical textbooks, considering genetic features, molecular mechanism, age of onset, clinical manifestations and course, response to treatment, and so on. Some are genetic and hereditary while others are acquired and not transmissible, the former are due, for example, to mutations in a chaperone gene, whereas the latter are due, for example, to post-translational modifications of the chaperone protein molecule. Since this is a new field, only recently defined within Medicine and that is not treated in most textbooks of Medicine or Pathology, the presentation will consist of an introductory overview. Various types of genetic and acquired chaperonopathies will be briefly discussed, considering that malfunctioning chaperones affect not only protein homeostasis (i.e., the canonical role of chaperones) but alto other unrelated cellular functions, pertaining for example, to cancer and autoimmune diseases. Some of these are classified as chaperonopathies by mistake or collaborationism. In them, one or more molecular chaperone, even if normal, actively favors disease, e.g., certain types of cancer require chaperones for cell growth and dissemination; in this context, data on Hsp60 chaperone migrations in cancer will be described. Acknowledgment: AJLM was partially supported by IEMEST. e-mail: [email protected] REDOX PROCESSES ASSOCIATED WITH PHYSIOLOGICAL PROTEIN FOLDING AND ENDOPLASMIC RETICULUM STRESS Francisco R. M. Laurindo Vascular Biology Laboratory, Heart Institute(Incor), University of São Paulo School of Medicine. Protein folding at the endoplasmic reticulum(ER) lumen involves chaperone-assisted folding, glycosylation and disulfide bond introduction, the later consisting in the transfer of oxidizing equivalents to cysteine thiols at their correct location in nascent proteins. The main effectors of disulfide bond introduction are protein disulfide isomerase(s), particularly PDIA1 (PDI). ER-based PDI is an abundantly expressed thioredoxin superfamily oxidoreductase displaying many interactions with redox and nonredox proteins and several post-translational modifications. PDI family contains >20 members with some apparent complementary actions. PDI has oxidoreductase, isomerase and chaperone effects, the latter not directly dependent on its thiols. PDI is a converging hub for pathways of disulfide bond introduction into ER-processed proteins, via hydrogen peroxide-generating mechanisms involving the ER flavooxidase Ero1α, as well as hydrogen peroxide-consuming reactions involving peroxiredoxin IV and novel peroxidases Gpx7/8. A situation in which ER-dependent reactive oxygen species (ROS) generation is increased in many cell types is ER stress. PDI is a candidate pathway for coupling ER stress to ROS generation. Emerging information suggests a convergence between PDI and Nox family NADPH oxidases. In vascular smooth muscle cells, PDI silencing prevents Nox responses to angiotensin-II and inhibits Akt phosphorylation in vascular cells. Also PDI is required for Nox1/ROSdependent vascular smooth muscle cell migration through pathways involving small GTPases Rac1 and RhoA, while PDI silencing promotes cytoskeletal disruption. PDI overexpression spontaneously enhances Nox activation and expression. During acute ER stress in smooth muscle cells, silencing of either Nox4 or PDI abolishes ROS generation, while PDI silencing enhances apoptosis. During sustained ER stress, ROS generation correlates with increased apoptosis, but does not induce cell death. At the cell surface, PDI mediates redox-dependent adhesion, coagulation/thrombosis, immune functions and virus internalization. The route of PDI externalization remains elusive. Thus, such multiple effects renders PDI(s) putative redox cell signaling adaptors of broader significance. Altogether, redox pathways associated with (patho)physiological protein folding are prime candidate reg ulators of cellular redox status in many diseases (Research supported by: FAPESP, CNPq/INCT Redoxoma). 64 Symp#13 Vascular cell biology Chair Robson Monteiro Tumor-Derived Microvesicles and their Role in Cancer Progression Robson Q. Monteiro Institute of Medical Biochemistry, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil Shedding of phosphatidylserine (PS)-containing microvesicles (MVs) by cancer cells have been correlated with several pro-tumoral responses. In addition, the procoagulant properties of MVs suggest their involvement in the establishment of cancer-associated prothrombotic states. Comparison of MVs produced by a non-tumorigenic melanocyte-derived cell line (melan-A) with its tumorigenic melanoma counterpart, Tm1, showed an increased rate of MVs production upon malignant transformation. Moreover, tumor-derived MVs displayed increased levels of the clotting initiator protein, tissue factor (TF). As a result, Tm1 but not melan-aderived MVs accelerated thrombosis in vivo. Analysis of plasma obtained from melanoma-bearing mice showed the presence of MVs with a similar procoagulant pattern as compared to Tm1 MVs produced in vitro. Remarkably, flow-cytometric analysis demonstrated that 60% of ex-vivo MVs are TF-positive and carry the melanoma-associated antigen, demonstrating its tumor origin. These data reinforce the possible involvement of tumor-derived MVs in the establishment of cancer-associated hypercoagulant states, indicating an important role for TF in this process. Since MVs may horizontally transfer their cargo between different cells, we further investigated the exchange of TF-bearing MVs between human breast cancer cell lines with different aggressiveness potential. Incubation of low aggressive MCF-7 cells with MVs from the aggressive cell line, MDA-MB-231, rendered a significant gain of TF activity. This phenomenon was not observed upon pretreatment of MVs with an anti-TF neutralizing antibody or annexin V, which blocks PS sites on MVs surface. These data indicate that TF-bearing MVs can be transferred between different populations of cancer cells, and thus may contribute to the propagation of a TF-related aggressive phenotype among heterogeneous cell subsets present in the tumor microenvironment. This study was supported by the Brazilian agencies CNPq and FAPERJ. Cell Adhesion and Signaling Pathways in Neurovascular Development Joseph H. McCarty Department of Cancer Biology, University of Texas M.D. Anderson Cancer Center, 1515 Holcombe Boulevard, Houston, Texas, 77030, U.S.A. The mammalian central nervous system contains billions of neurons and glia that are interlaced with an elaborate network of blood vessels comprised of endothelial cells, pericytes and vascular basement membranes. During development blood vessels grow and sprout along a pre-formed latticework of glial cells; however, the mechanisms by which glial cells control central nervous system neovascularization remain enigmatic. We have used Cre-lox strategies in mice to demonstrate that αvβ8 integrin expressed in glial cells is essential for neovascularization of the developing central nervous system. Cell type-specific inactivation of αv or β8 integrin gene expression in radial glia using a Nestin-Cre transgene leads to the development of hemorrhagic blood vessels that form glomeruloid-like tufts in the embryonic brain and the neonatal retina. These pathologies correlate with diminished activation of latent TGFβs, which are extracellular matrix-bound protein ligands for αvβ8 integrin. Genetic ablation of canonical TGFβ receptors Alk5 or TGFβR2 in vascular endothelial cells during embryogenesis result in brain vascular pathologies that are identical to those in integrin conditional knockout mice. Furthermore, tamoxifen-inducible inactivation of TGFβ receptor signaling in retinal endothelial cells also leads to defective angiogenesis and intraretinal hemorrhage. Collectively, our data demonstrate that αvβ8 integrin and TGFβ receptors are components of a paracrine signaling axis that links glial cells to endothelial cells during central nervous system vascular development. Vascular growth factor signaling in neurogenesis Jean-Léon Thomas#, Anne Eichmann* Departments of Neurology# and Cardiovascular Medicine*, Yale School of Medicine, New Haven, CT, USA # Brain and Spinal Cord Institute, Paris, France In the adult mammalian brain, the potential to generate new neurons is restricted to a limited number of sites called neurogenic niches, which are localized in the subventricular zone (SVZ) lining the cerebral ventricles and in the dentate gyrus (DG) of the hippocampus. Injury of brain tissue resulting from trauma or pathologies activates neurogenesis in these niches, attesting to an endogenous repair potential that is generally not sufficient to allow a complete rescue. To enhance this endogenous neurogenic response without negative side effects, it is crucial to characterize the mechanisms which are active in neurogenic niches. Functionally, members of the vascular endothelial growth factor (VEGF) family stimulate adult neurogenesis and neuronal plasticity, opening potential approaches for repair of neurodegenerative diseases. However, it has been unclear whether VEGFs stimulate neurogenesis directly via VEGF receptors (VEGFRs) expressed by neural cells, or indirectly via the release of growth factors from angiogenic capillaries. We have reported that the lymphangiogenic growth factor VEGF-C is expressed by neural cells and provides trophic support to neural progenitor cells during brain development (Le Bras, Nat Neurosci, 2006). Here, we will discuss our latest findings on its receptor VEGFR-3, which is expressed by adult NSCs, and is critical for adult neurogenesis by acting directly in NSCs and niche astrocytes, but not endothelial cells (Calvo, Genes Dev., 2011). 65 Symp#14 Cell cycle control mechanisms Chairs Patricia Gama and Hugo Aguirre Armelin Stabilizing nuclear p27kip1 with Skp2/Cks1 E3 ligase inhibitors as a potential therapeutic intervention for endometrial cancer and other cancers Savvas C. Pavlides, Lily Wu, Kuang-Tzu Huang, Stepahnie V. Blank, Khushbakhat Mittal, Timothy Cardozo, and Leslie I. Gold School of Medicine, New York University, USA The cell cycle is precisely regulated via the ubiquitin-proteasome system (UPS) by three substrate-specific E3 ubiquitin ligases, APCCdc20, APC-Cdh1, and SCF-Skp2/Cks1. Inhibitors of specific E3 ligases that degrade proteins involved in cell cycle arrest are significant targets to block for cancer therapy and a significant improvement over currently used non-specific proteasome inhibitors. The cyclindependent kinase inhibitor, p27kip1 (p27), is important for cell cycle arrest in G1. SCF-Skp2/Cks1 ubiquitylates nuclear p27 targeting it for degradation thereby causing cell cycle progression. In turn, APC-Cdh1 signals Skp2 and Cks1 degradation, maintaining abundant levels of p27 for cell cycle arrest. We show perpetual degradation of p27 by the UPS in type I endometrial carcinoma (ECA), an estrogen (E2)-induced cancer. Using normal human primary endometrial epithelial cells (EECs), we demonstrate that E2 induces MAPK-Erk2-driven ubiquitin-mediated degradation of p27 by its phosphorylation at T187, required for its degradation by SCFSkp2/Cks1. Also, E2 decreases APC-Cdh1 to increase Skp2 and Cks1 levels for p27 degradation. We propose that E2-induced degradation is involved in the pathogenesis of ECA because knocking-down Skp2 completely blocks E2-induced p27 degradation and growth stimulation. Conversely, progesterone (Pg) and TGF-β, both inhibitors of EEC growth, markedly increase p27 by increasing Cdh1 thereby causing destruction of Skp2/Cks1 leaving p27 intact. Accordingly, separately knocking-down Cdh1 and TGF-β obviate both Pg- and TGF-β-induced stabilization of nuclear p27 and completely blocks their ability to inhibit growth. These studies suggest that preventing p27 degradation is a rational therapeutic strategy for the treatment of type I ECA. Indeed, small molecule inhibitors of Skp2 E3ligase activity (Skp2E3LIs) shown in silico to block p27 binding to Skp2-Cks1, inhibit both E2-induced proliferation and degradation of p27 in an ECA cell line and in primary ECA cells. Significant progress has been made in vitro showing lack of toxicity and that certain Skp2E3LIs specifically block nuclear degradation of p27 where it can inhibit Cdk1 for cell cycle arrest. Skp2E3LIs provide tools for understanding the role of the UPS in p27-mediated cell cycle regulation and as a novel approach to treating ECA and many other cancers with inverse levels between p27 and Skp2. Cyclin F-mediated degradation of RRM2 (Ribonucleotide Reductase family member 2) controls genome integrity and DNA repair Vincenzo D’Angiolella1, Valerio Donato1, Frances M. Forrester1, Yeon-Tae Jeong1, Claudia Pellacani1, Yasusei Kudo1,2, Anita Saraf3, Laurence Florens3, Michael P. Washburn3,4, and Michele Pagano1,5 1 Department of Pathology, NYU Cancer Institute, New York University School of Medicine, 522 First Avenue, SRB 1107, New York, NY 10016, USA. 2Department of Oral Molecular Pathology, Institute of Health Biosciences, The University of Tokushima Graduate School, Tokushima, Japan. 3The Stowers Institute for Medical Research, 1000 East 50th Street, Kansas City, MO 64110, USA. 4Department of Pathology and Laboratory Medicine, The University of Kansas Medical Center, 3901 Rainbow Boulevard, Kansas City, Kansas 66160, USA. 5Howard Hughes Medical Institute F-box proteins are the substrate recognition subunits of SCF ubiquitin ligase complexes. The F-box protein family obtained its name from Cyclin F (also known as Fbxo1), in which the F-box motif was first described. Cyclin F is localized both to the centrosomes and the nucleus. At the centrosomes, Cyclin F targets CP110 for proteasomal degradation during G2 to limit centrosome duplication to once per cell cycle. Instead, the nuclear function of Cyclin F remains elusive. Using purifications and mass spectrometry, we identified RRM2 (the ribonucleotide reductase family member 2) as a new interactor of the F-box protein Cyclin F. Ribonucleotide reductase (RNR) catalyzes the conversion of ribonucleotides to the deoxyribonucleotides (dNTPs) that are necessary for replicative and repair DNA synthesis. Because of this fundamental function, RNR is among the most well-conserved (from prokaryotes to eukaryotes) and highly-regulated enzymes. Indeed, an unbalanced and/or increased dNTP pools produce a hypermutator phenotype, and decreased dNTP levels interfere with proper DNA replication and repair. We found that, during G2, following CDK-mediated phosphorylation of Thr33, RRM2 is degraded via SCFCyclin F to maintain balanced dNTP pools and genome stability. After DNA damage, Cyclin F is downregulated in an ATR-dependent manner to allow accumulation of RRM2. Defective elimination of Cyclin F delays DNA repair and sensitizes cells to DNA damage, a phenotype that is reverted by expressing a non-degradable RRM2 mutant. In summary, we have identified a novel biochemical pathway that controls the abundance of dNTPs and ensures efficient DNA repair in response to genotoxic stress. Resveratrol and Temozolomide co-treatment induces mitotic catatrophe and senescence in glioma cells through modulation of mitotic regulators Eduardo C. Filippi-Chiela and Guido Lenz* Department of Biophysics and Center of Biotechnology, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil. Email: [email protected] Phone: 55 51 33087613 Blockage of the cell cycle is an important strategy in cancer therapeutics. On the other hand, forcing mitosis in cells with high levels of DNA damage may also be a good strategy, since it may induce mitotic catastrophe (MC) and senescence. Temozolomide (TMZ), the primary therapy used in gliomas, causes DNA damage and G2 arrest. Resveratrol (Rsv) presents additive toxicity with TMZ in several glioma cells in vitro and in vivo, but the mechanism of additive toxicity is not clear, which is the aim of the present work. Rsv abrogated the TMZ-induced G2 arrest when added together, but not after TMZ. Rsv potentiated the increase in TMZ-induced gammaH2AX, but not ATM and Chk2. Abrogation of TMZ-induced cell cycle arrest by Rsv involved a reduction of cyclin D, pWee1(S642) and of the Wee1 target site, pCdc2(Y15) and increase of cyclin B. This suggests a state of forced passage through G2 checkpoint despite large DNA damage, a scenario typical of MC. In order to quantify MC and senescence, we developed a quantitative method called nuclear morphometric analysis (NMA). Indeed, after acute treatment with Rsv+TMZ, the proportion of cells with high nuclear irregularity increased from 5 to 28% in 48h. Seven days later, a large induction of senescence and reduction in clonogenicity was observed. In conclusion, presence of Rsv forced damaged cells treated with TMZ through mitosis due to a reduction of Wee1 and pCdc2(Y15), leading to MC and senescence. Funding: CNPQ and FAPERGS. No conflict of interest. 66 Symp#15 Migration and Regeneration Chair Fernando Costa e Silva Filho A mechanochenical cross-talking between eukaryotic cells and their surroundings instruct cells on what they have to do 1* 2 1 1,3 Fernando Costa e Silva Filho , Nathan Bessa Viana , Lilian de Mello Gil , and Débora Barreiros Petrópolis 1,2 1 2 3 UFRJ- Instituto de Biofísica Carlos Chagas Filho and Instituto de Física (Brazil), Institute Pasteur (France), 1,3 and INCT- Instituto Nacional de Pesquisa Translacional em Saúde e Ambiente da Região Amazonica (Brazil) Collagen I (COL) is abundant in the extracellular matrix (ECM) of most of studied animal tissues and one of the most widely-used scaffolds for three-dimensional (3D) cell culture and tissue engineering applications, which is partly derived from the ability of purified COL monomers to self-assemble into stable, 3D gels at physiological pH. The fiber diameter, pore size, and bulk elasticity of reconstituted COL gels can be tuned within modest ranges by changing COL concentration, ionic strength, pH, and the temperature of gelation. The last in turn begins with the entropy-driven nucleation of triple-helical COL monomers into small aggregates, which subsequently self-assemble into thin filaments that laterally crosslink into the well known COL fibers. A 3D COL matrix is then formed via non-covalent entanglement of the fibers. As a consequence of this entanglement, reconstituted COL networks or meshes typically exhibit nonaffine mechanical properties which means applied stresses are dissipated non-uniformly throughout meshes via sliding, slipping, bending, and bucking of individual COL fibers. Given such 3D COL properties which partially mimic the mechanical configuration of naturally occurring ECM we have been used COL meshes under different mechanical configurations to explore further the responsiveness of some protozoa and mammalian cells to the mechanics of their surroundings. Trophozoitic forms of the parasitic protozoan E. histolytica (HM1:IMSS) and human osteoblasts (HOB) are cells we have elected to investigate the mechanisms underlying the interaction between eukaryotes and each one of 2D (biofilms) and 3D (meshes) COL setups by using biophysical, biochemical, structural and ultrastructural methods as well as optical tweezers. Altogether, the resulting data we have obtained clearly show that the early response of a protozoan and a mammalian cell to a same mechanochemical environment is quite different: while HM1:IMSS cells tend to use COL fibers as migration tracks, HOB cells tend to remodel the mesh at high extent following invasion. Inside-out integrin signaling Mark Ginsberg University of California, San Diego, Department of Medicine, La Jolla, CA Integrin activation contributes to leukocyte trafficking, cell migration and extracellular matrix assembly. Deletion of talin or point mutations in talin or integrins that disrupt their interaction led to profound defects in integrin activation. We reconstructed integrin activation in vitro and found that that talin binding is sufficient for activation. Talin interaction with phospholipids is required for its capacity to activate integrins. Nanodiscs bearing a single lipidembedded integrin and revealed that talin activates unclustered integrins leading to molecular extension in the absence of force or other membrane proteins. Rap1 small GTPases are important in activation of integrins and we report that they interact with RIAM (Rap-interacting Adaptor Molecule) to promote talin-dependent activation. RIAM connects the membrane targeting sequences in Ras GTPases to talin, thereby recruiting talin to the plasma membrane and activating integrins. A minimized 50 residue Rap-RIAM module, containing the talin binding site of RIAM joined to the membrane-targeting sequence of Rap1A is sufficient to target talin to the plasma membrane and to mediate activation in the absence of Rap1 activity. The structure of the αIIbβ3 transmembrane domain (TMD) reveals how talin binding can disrupt the αβ TMD complex and lead to the long range conformational change that results in integrin activation. These studies define the molecular mechanisms whereby talin activates integrins and establish a signaling roadmap between agonist stimulation and integrin activation. Human-laminin mediates axonal regeneration promoted by human adipose tissue-derived stromal cells after spinal cord injury in rats Tatiana Coelho Sampaio Universidade Federal do Rio de Janeiro, Instituto de Ciências Biomédicas, Departamento de Histologia e Embriologia, Rio de Janeiro, Brasil Adipose tissue is a convenient source of adult mesenchymal cells for regenerative therapies. We injected human adipose tissue-derived stromal cells (hADSC) after acute spinal cord injury in immunocompetent rats and found that they promoted extensive morphological and functional recuperation. In contrast to controls, treated animals presented 1) clusters of neural precursors in the spinal parenchyma, 2) blood vessels with double basement membranes and 3) abundant deposition of laminin of human origin at the lesion site and spinal midline. These effects did not occur upon treatment with conditioned medium, but did occur after injection of hADSC into the non-injured cord. Fibers positive for 5-HT, Beta III tubulin or GAP-43 were visible in the tissue surrounding the cystic cavity in close association with laminin. We propose that laminin is the main effector of hADSC-induced axonal regeneration and that it acts by increasing the amount of local neural precursors. 67 Symp#16 Inflammation Chair Patrícia Bozza Patrícia Bozza Instituto Oswaldo Cruz, FIOCRUZ, Rio de Janeiro, Brazil Introductory notes The intimate link between fibrosis and inflammatory response Niels Olsen Saraiva Camara Department of Immunology, Institute of Biomedical Sciences, University of Sao Paulo, São Paulo, Brazil Toll-like receptors (TLRs) are an innate family of receptors that can sense tissue damage and orchestrate a cascade of inflammation. Recent reports have shown reduced fibrosis in TLR4-deficient mice. TLR2 and TLR4 signal via the intracellular adaptor molecule MyD88, although only few studies implicated a role for MyD88 in fibrosis. TLRs modulate the immune system through the production of different cytokines and influence fibrosis. In fact, fibrosis is strongly linked with the development of a Th2-biased response (involving IL4, IL5 and IL13). Macrophages are considered to play a pivotal role in the development of fibrosis. Recent studies raise the possibility that the effector phenotype of the recruited macrophages, rather than their presence, determines the extent of renal parenchymal injury. Macrophages are classified in distinct subpopulations according to their response to innate or adaptive immune signals. The term “classically activated” has been used to designate the effector macrophages that are produced during cell-mediated immune responses. Such macrophages are also designated M1 macrophages and express iNOS, CXCL9, CCR7, CXCL11, IL12 and IFNγ. On the other hand, one of the first innate signals released during tissue injury is thought to be IL4, an inducer of “alternatively activated” or M2 macrophages. Since collagen deposition is a hallmark of all chronic diseases, preceded by the development of sterile inflammation, which can be modulated by the presence of cytokines, here, we present data that MyD88-depend pathway could be involved in sensing these tissue alterations and in favoring a Th2-prone pro-fibrotic immune response. How a Parasite MIF Suppresses T cell Immunity and Influenced the Evolution of Macrophage Responsiveness Rick Bucala MD PhD, Yale University, New Haven, CT. The inability to acquire protective immunity against Plasmodia is the chief obstacle to malaria control, and an inadequate T cell response may contribute to persistent blood stage infection. We observed that high levels of inflammatory cytokines inhibit Plasmodium-specific memory T cell development and result in fewer protective memory T cells. The Plasmodium ortholog of macrophage migration inhibitory factor (MIF), which is produced during infection, is associated with inflammatory sequelae in human malaria and induces high levels of pro-inflammatory cytokine expression. Using a genetically targeted strain of P. berghei, the Plasmodium MIF (PMIF) mediated increase in inflammatory cytokine expression was found to promote T cell apoptosis, resulting in fewer antigen-experienced CD4 T cells that become memory cells. CD4 T cells activated in the presence of PMIF fail to produce robust anti-malaria recall responses during a secondary challenge infection and are unable to control parasitemia. These results indicate that Plasmodia modulate the adaptive immune response and interfere with the generation of malaria-specific memory CD4 T cells, thereby facilitating parasite persistence and transmission. The immunoregulatory function of PMIF may account for its expression across all Plasmodium spp., its evolutionary conservation in protozoan and helminthic parasites, and the prevalence of low expression MIF alleles in many human populations. Targeting PMIF may be a useful approach for augmenting natural host immunity and for producing more effective vaccines. 68 Symp#17 Glia Chair Flávia Carvalho Alcântara Gomes GLAST/EAAT1 induces Glutamine release through SNAT3 in cultured chick cerebellar Bergmann glial cells 1 2 Angelina Rodriguez and Arturo Ortega 1 2 Facultad de Química, Universidad Autónoma de Querétaro, Querétaro, Mexico., Departamento de Genética y Biología Molecular, Cinvestav-IPN, México DF, Mexico. Glutamate is the main excitatory neurotransmitter in the vertebrate brain. Once released, its extracellular levels are tightly regulated through the action of a family of sodium-dependent glutamate/aspartate transporters profusely expressed in glial cells. Once internalized into the glial compartment, it is metabolized by glutamine synthetase to glutamine and released to the synaptic space through sodium-dependent neutral amino acid carriers of the N System. Glutamine is then taken up by neurons via System A transporters completing the so-called glutamate/glutamine shuttle. Although this neuronal/glial coupling was described decades ago, it has only been recently that the biochemical framework that supports this shuttle has begun to be elucidated. Using the established model of cultured cerebellar Bergmann glia cells from chick cerebellum, we characterized the functional and physical coupling of glutamate uptake and glutamine release. A time-dependent glutamate transporter-induced glutamine release could be demonstrated. Furthermore, D-aspartate, a specific glutamate transporter ligand, was capable to enhance the coimmunoprecipitation of the glutamate and glutamine transporters, whereas glutamine tended to reduce this association. Our results clearly pointout that glial cells that enwrap glutamatergic synapses act as sensors of neuronal activity and through their contribution to the neurotransmitter recycling, could well the rate-limiting step of glutamatergic synaptic function. Understanding Neuron-Glia Interactions: Models Matter Frank W. Pfrieger Institute of Cellular and Integrative Neurosciences, CNRS UPR 3212, University of Strasbourg, Strasbourg, France Brain development and function depend on interactions of neurons and different type of glial cells. Within the last years, we have developed new experimental approaches that allow to study these interactions in vitro and in vivo, with a focus on the retina. In my presentation, I will summarize our results, which indicate contributions of astroglial cells to synapse development, neuronal volume regulation and cholesterol homeostasis in the brain. Adan Aguirre Department of Pharmacological Sciences, 442 Center for Molecular Medicine, Stony Brook University, , Stony Brook, NY 11794-5140 69 Symp#18 Perspectives in cancer therapies Chair Jörg Kobarg Prospecting and testing new molecular target proteins for cancer therapy: integrating systems and structural biology” Jörg Kobarg, PhD LNBio-Laboratório Nacional de Biociências, CNPEM-Centro Nacional de Pesquisa em Energia e Materiais Rua Giuseppe Máximo Scolfaro 10.000 Campinas-SP, Brasil, CEP 13083-970 F: 0055-19-3512-1125 Starting from identified cancer related proteins or candidate proteins we explore and integrate several techniques and approaches ranging from structural biology, micro array, proteomics, protein interactome to cellular and molecular functional characterization, in order to obtain information on the mechanisms underlying the dysfunction of these proteins in cancer and to envision new modes of interference aiming at the target specific therapeutic intervention in cancer. Distinct aspects of this approach are exemplified by three different proteins or groups of proteins currently under investigation: 1. FEZ1 as a kinesin associated transport adaptor protein that when over-expressed can lead to the formation of so called “flower-like nuclei“, a hall mark of certain aggressive sub-types of leukemia. 2. Il-7 Receptor Cys insertion mutations that lead to aberrant receptor homo-dimerization and constitutive activation , growth and survival of lymphocytes and to tumor formation in the mice model. 3. The 11 human members of the family of NIMA (Never in mitosis gene A)-related serine/threonine kinases (Neks) have cell cycle-related functions, were recently described as related to pathologies, particularly cancer, and present promissing chemotherapeutic targets. In order to understand better the cellular functions of human Nek kinases we performed yeast two-hybrid assays using Nek1, 6, 7 and 9 as baits to identify their protein interaction partners. Similar studies are currently ongoing for Nek 3, 4, 5, 10 and 11. We will present a general overview of our results and their implications for these protein kinases functions in the context of tumorigenesis. Modern optical techniques for diagnostic and treatment of cancer and microorganisms Vanderlei S. Bagnato Instituto de Física de São Carlos, Universidade de São Paulo, São Carlos, Brazil Solving health problems with photonics techniques is attractive due to its more selective and fast response, minimally or non-invasive procedure, and potential low cost instrumentation. These characteristics are especially relevant for emergent economy countries, where health care is still deficient for large amount of population. Brazil shows a diverse situation along its large territory: it is possible to find the best medicine with the highest technology available and well educated personnel, but also a poor health care or even the lack of one. Diagnostics and treatment techniques that are effective, low cost, and that requires simple instrumentation may be good solutions for improving health care. This presentation will start with the main principles involved in photonics for live science and present the status of development for applications in cancer diagnostic and treatmne as well as microbial control. Deciphering Neuregulin-HER signaling in breast cancer Atanasio Pandiella Centro de Investigación del Cancer. CSIC-Universidad de Salamanca, Spain. The ErbB/HER receptors and their ligands play important roles in animal physiology, and their deregulation has been linked to diseases such as cancer. Activation of the HER receptors may occur by different mechanisms, including ligand binding, receptor overexpression, or molecular alterations. In breast cancer, one of the HER family recptors, termed HER2, is overexpressed in tumors of 20% of patients, and this has led to the development of therapies against HER2 which are now routinely used in the breast cancer clinic. While assessment of the levels of HER receptors in breast cancer has been extensively analyzed, the role of their ligands has been less well studied. We have explored the expression of Neuregulins (NRGs), a subgroup of HER ligands, in breast cancer samples. We observed frequent expression of these ligands, and such expression was linked to metastatic dissemination and poor clinical outcome, indicating that targeting this ligand system may be therapeutically beneficial. For this reason, we have started a program to identify how the NRG-HER signaling system controls the proliferation of breast cancer cells. Genomic as well as proteomic strategies have allowed us to identify novel signaling intermediates of the NRG-HER system. Using biochemical, genetic, cell biological techniques, as well as xenografted mice, we have elucidated the participation of these novel signaling intermediates in NRG-stimulated proliferative responses in breast cancer cells. Pharmacological action on some of these intermediates allowed us to evaluate the potential therapeutic value of their targeting in breast cancer. 70 Symp#19 Regulators of neural transmission Chairs Vilma R Martins and Roy Larson Regulation of neuronal function and dysfunction by protein SUMOylation Jeremy M. Henley University of Bristol The post-translational modification SUMOylation is a major regulator of protein function that plays an important role in a wide range of cellular processes. SUMOylation involves the covalent attachment of a member of the small ubiquitin-like modifier (SUMO) family of proteins to lysine residues in specific target proteins via an enzymatic cascade analogous to, but distinct from, the ubiquitination pathway. The implications for neuronal protein SUMOylation are far-reaching in both normal cell function and in neurological and neurodegenerative diseases. I will discuss aspects of our work attempting to identify and functionally characterise SUMO substrates; elucidate the molecular mechanisms regulating, and consequences of, substrate SUMOylation and deSUMOylation; determine the activity-dependence of SUMO and SUMO-specific protease trafficking to synapses; and define how SUMOylation regulates synaptic transmission under basal, stimulated and pathological conditions. Gain control in the outer retina 1,2 1 Joselevitch, C. , Kamermans, M. 1 - Retinal Signal Processing, The Netherlands Institute for Neuroscience; The Netherlands. 2 - Department of Experimental Psychology, Universidade de São Paulo, Brazil. Gain control mechanisms are present at all retinal layers. They are especially important for cells that receive mixedinput from rods and cones, in order to avoid premature saturation as light levels increase. Here we describe a mechanism at work in the goldfish retina that modulates the effectiveness of the rod-bipolar cell synapse. Voltageclamp recordings of mixed-input ON bipolar cells in retinal slices show that a voltage-gated current is activated during the light-induced depolarization at scotopic levels. The activation of this current effectively diminishes the amplitude of the bipolar cell rod-driven light response and makes it faster and more transient with increasing light intensity. This + + effect can be abolished by the K channel blocker TEA, which indicates that the voltage-gated current is mediated by K + ions. Mathematical simulations with NEURON suggest that the K channels are most likely concentrated at the dendritic tips of mixed-input ON bipolar cells, close to the site of glutamate release by photoreceptors. Since the magnitude of activation of such channels depends directly on the amplitude of the light response, they control the gain of the rod bipolar cell synapse and speed up synaptic transmission as light levels increase and rod responses themselves inactivate slowly. Protein synthesis and memory processing Martin Cammarota Instituto de Pesquisas Biomédicas, PUCRS, Porto Alegre, Brazil 71 Symp#20 Tissue Regeneration Chair Juan Larrain Spinal cord regeneration in Xenopus 1 1 1, 1 2 1 Rosana Muñoz , Dasfne Lee-Liu Mauricio Moreno , Gabriela Edwards , Leonardo I. Almonacid , Victor Tapia , Karina 1 2 1 Tapia , Francisco Melo , Juan Larrain 1 Center for Aging and Regeneration and Millenium Nucleus in Regenerative Biology, Department of Cell and Molecular 2 Biology, Pontificia Universidad Catolica de Chile; Molecular Bioinformatics Laboratory, Millennium Institute on Immunology and Immunotherapy, Department of Molecular Genetics and Microbiology, Pontificia Universidad Catolica de Chile. Xenopus tadpoles are able to regenerate the spinal cord (SC) after injury, although this capacity is lost when they reach metamorphosis. The cellular and molecular mechanisms that explain this differential SC regeneration ability have not been unveiled (or are not completely understood). Thus, a deep comparison between the regenerative capacities in these two stages of Xenopus life cycle might be crucial to establish why SC regeneration is lost during metamorphosis. + We have found that spinal cord transection activates proliferation of Sox2 cells from the ependymal layer in + regenerative but not in non-regenerative stages. At 6 days post transection (dpt) Sox2 cells fill the gap between the spinal cord stumps and provide a surface for axonal regeneration.To deepen this analysis, we propose that the differences in regeneration capacity can be explained at least at the level of gene expression. Thus, we aim to identify a group of SC transcripts that are permissive and another non-permissive for regeneration. To demonstrate this we performed a high-throughput analysis of the SC transcriptome (RNA-Seq) after injury (transection) in different stages. So far, we have found that more than 4000 transcripts show differential expression when comparing regenerative and non-regenerative stages. We have successfully validated these differences in a group of them using qRT-PCR. And importantly, gene ontology enrichment analyses show that genes belonging to the biological processes ‘cell cycle’ and ‘immune response’ are differentially regulated after spinal cord injury, amongst others. Considering these preliminary results we suggest that the differences in SC regeneration capacities in Xenopus could be explained at least by a differential expression of cell cycle and immune response genes. Cellular and Molecular Mechanisms of Zebrafish Heart Regeneration Ken Poss HHMI, Duke University Medical Center, Durham, USA Cellular and molecular mechanisms of intestinal regeneration in echinoderms Jose E. García-Arrarás University of Puerto Rico In recent years we have seen a growing interest in determining the cellular and molecular mechanisms involved in the process of organ regeneration. This process comprises a sequence of temporal and spatial events that interact to give rise to a new organ. We have studied the regeneration of the digestive tract using as a model system the sea cucumber Holothuria glaberrima. This echinoderm, like many other holothurians, has the capacity to regenerate most of its digestive tract following its loss. We have shown that the new intestinal primordium is a blastema-like structure that forms as the mesenterial cells undergo a series of changes that include dedifferentiation, proliferation and apoptosis. These cells undergo changes at the molecular level, expressing molecular markers not normally found in the normal mesentery. Among the molecular markers that appear to be over-expressed within the epithelial cells during the formation of the intestinal rudiment are those associated with signalling pathways such as Wnt 9 and BMP1/TLD, with the control of cellular proliferation and cell death such as TCTP, survivin, and mortalin and with the ubiquitinproteasome system. The drastic changes observed in the expression profile of the epithelial component suggest that these cells are the key players in the regeneration process and provide a target to further explore their role in organ regeneration. Thus, as we unravel the cellular and molecular mechanisms associated with intestinal regeneration in echinoderms we provide a much-needed insight into the basic biological processes involved in organ regeneration. Funded by NIH (1SC1GM084770), NSF (IOS-0842870) and the University of Puerto Rico 72 Symp#21 Metabolic programming Chair James Armitage Maternal obesity, diabetes or high fat intake in pregnancy: Are they all independent risk factors for metabolic syndrome in her offspring? James A Armitage Department of Anatomy and Developmental Biology Monash University, Victoria, Australia In many societies across the globe, more than half of the women of reproductive age are overweight or obese. Maternal obesity and diabetes in pregnancy have long been recognised as risk factors for adverse pregnancy outcomes, including emergency caesarean section, shoulder dystocia and perinatal complications. More recently we have also come to appreciate the fact that maternal obesity or diabetes may also programme metabolic, cardiovascular and renal dysfunction in her offspring. Our studies in diabetic mice show that maternal hyperglycaemia programmes abnormal fetal growth and a reduction in kidney development, which occurs from the earliest time points of kidney development and may result in lifelong alterations in renal function. In addition to the obesity epidemic, humans across the globe are consuming diets very high in fats and oils, particularly saturated fatty acids. At present it is not known whether consumption of a high fat diet is sufficient to programme metabolic cardiovascular or renal disease in the offspring. We developed a rodent model to better understand the role of maternal fat intake in pregnancy without the confound of maternal obesity and show that high saturated fat intake in pregnancy and suckling programmes offspring hypertension and altered renal function independent of maternal or offspring obesity. The mechanisms underlying this programming may relate to placental fatty acid transfer and cytokine production in late gestation. In conclusion, maternal diabetes and excessive maternal saturated fatty acid intake can programme alterations in kidney development and function independent of maternal obesity. Given the preponderance for high saturated fat intake, and the increasing prevalence of diabetes in women of reproductive age, these findings offer strong evidence for moderation of fat intake in pregnancy and careful management of hyperglycaemia in pregnant women. SMOKING IN THE POSTNATAL LIFE AND FUTURE OBESITY: the nicotine role on the endocrine dysfunctions. Patricia Cristina Lisboa Laboratory of Endocrine Physiology, Department of Physiological Sciences, Biology Institute, State University of Rio de Janeiro, RJ. Around 40% of children worldwide are exposed to tobacco smoke at home. Children born from smoking mothers present several developmental impairment. Environmental changes in a critical window of development, such as gestation or lactation, can cause permanent alterations in the metabolism, leading to disease at adulthood; a phenomenon called programming or developmental plasticity, which is based on epigenetic alterations (DNA methylation and histone acetylation) that change the pattern of expression of several genes involved with the metabolism regulation. A histone deacetylase, Sirt1 is inhibited by nicotine and can play an importante role in the developmental plasticity. Obesity is a global epidemic and it has been shown an association of maternal smoking with the development of obesity in childhood. However, little is known about the early and late effects of the tobacco in neonatal life upon adiposity and endocrine function. We studied two models of programming related to smoking that produced animals with higher cardiovascular risk and endocrine dysfunction during development: maternal nicotine exposure (i) and maternal cigarette smoke exposure (ii), both only during lactation. We evidenced a relationship between hyperleptinemia in offspring whose mothers were exposed to nicotine during lactation with the further development of leptin and insulin resistance as well as thyroid and adrenal dysfunctions in adulthood. Thus, an environment free of smoke during lactation is essential to improve health outcomes in adult life, reducing the risk for future diseases. The knowledge about the pathophysiological mechanisms involved in maternal smoking can give new insight concerning therapeutic strategies for obesity. Diet-induced hypothalamic inflammation in obesity Licio A. Velloso University of Campinas, Brazil Obesity results from the failure of the homeostatic control of caloric intake and energy expenditure. Most of this control is exerted by a complex network of hypothalamic neurons that integrate hormone, nutrient and neuronal signals involved in the sensing and responsiveness to the fluctuations of the body energy stores. Data obtained in the latest fifteen years have placed hypothalamic dysfunction in a central position in the pathogenesis of obesity. Here, we will review the seminal work that have contributed to our current knowledge of the mechanisms involved in the control of food intake and thermogenesis and also the implications of hypothalamic dysfunction in the development of obesity. We will present data from both experimental and human studies showing that saturated fats present in the western diet can activate hypothalamic inflammation which results in the defective control of energy homeostasis. 73 Symp#22 Mitochondria Chairs Enilza Espreafico and Nadja Souza-Pinto Evidence implicating KIAA0090/CG2943 in mitochondrial function ENILZA M ESPREAFICO, RODRIGO R SILVA, CARLOS A COUTO LIMA, ROBERTO A. MOLINA, JOSANE F SOUSA, MILENE M LOPES, MAIARO C MACHADO, LUCAS ANHEZINI, RICARDO GP RAMOS Departamento de Biologia Celular e Molecular e Bioagentes Patogênicos, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, 14049-900 - Ribeirão Preto, SP, Brasil Human KIAA0090 is an evolutionarily conserved and ubiquitously expressed gene that maps to a chromosomal region (1p36.13) with frequent aberrations in cancer. It is a complex gene with cDNA sequences in databases supporting the occurrence of more than 20 alternative transcripts. The RefSeq transcript is predicted to encode a 993 aa transmembrane protein whose S. cerevisiae ortholog (EMC1) was recently proposed to function on transmembrane protein folding in the endoplasmic reticulum (ER). Deletion of the gene in yeast and C. elegans leads to slow growth and a number of interactors involved in multiple pathways, including cell cycle, secretory pathway, UPR, ERAD, ion transport, cytoskeleton, transcription factors, and mitochondrial electron transfer, have been detected. We found KIAA0090 to be upregulated in melanoma cells and nevi. Expression of EGFP-tagged proteins in mammalian cells showed pronounced apoptotic cell death and involved alterations in mitochondria and ER. KIAA0090 knockdown also led to an increase of cell death rates in melanoma cells. The endogenous protein was primarily localized either to mitochondria or Golgi, depending whether the antibody used was to the N- or C-terminal regions. The results shown here corroborate many genetic interactions found in yeast, but suggest that KIAA0090 protein has a broader subcellular localization and function than the one proposed for its yeast counterpart. To decipher the role of this gene in development, we are beginning to conduct functional studies on the Drosophila melanogaster KIAA0090 ortholog, CG2943. Driving an RNAi targeting CG2943 mRNA to skeletal muscle led to high rates of pupal lethality and generated offsprings unable to fly and with severe deficiency of locomotion. Ultrastructural analyses of muscle fibers are currently being performed to gain insights into the structural basis that accounts for the observed phenotype. Financial Support: FAPESP, CNPq, DECIT, CAPES, FAEPA Mitochondrial BER activities maintain mtDNA stability and mitochondrial function Nadja Souza-Pinto Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, SP, Brazil The mammalian mitochondrial DNA (mtDNA) codes for 13 polypeptides, all essential components of the electron transfer chain. Mutations and deletions of the mtDNA lead to mitochondrial dysfunction, which cause several human syndromes and have been implicated in common, multi-factorial diseases such as cancer and neurodegeneration. The mtDNA is closely associated with the inner mitochondrial membrane, where reactive oxygen species are generated as byproducts of normal oxidative metabolism. Thus, mitochondria rely on DNA repair pathways to maintain mtDNA stability. Among those, the base excision repair has been extensively characterized in mammalian mitochondrial. Mitochondrial BER (mtBER) involves 5 enzymatic steps, catalyzed by isoforms of the enzymes involved in nuclear BER, which have been biochemically characterized. However, its regulation is still unclear. We hypothesized that mtBER activity is modulated by proteins interactions, in a fashion akin to the modulation of nuclear BER. Using in vitro assays to measure each BER step independently, we have identified two proteins which strongly affect mtBER activity. The repair factor CSB (mutated in Cockayne Syndrome) is involved in maintaining mtBER activities anchored to the inner mitochondrial membrane, where the mtDNA is located, and thus, favors mtDNA repair efficiency. On the other hand, the nucleoid protein TFAM (Mitochondrial transcription factor A) binds to damaged DNA, diminishing the accesses of repair enzymes. TFAM binding to DNA is modulated by p53, allowing the damage to be accessed by the BER enzymes. We propose a model in which CSB, TFAM, p53, and others, yet unidentified proteins, modulate mtBER activity in response to stress. Dietary interventions, mitochondria, oxidants and lifespan Alicia J. Kowaltowski Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, SP, Brazil Mitochondrial energy metabolism and mitochondrially-derived oxidants have, for many years, been recognized as central toward the effects of aging. Calorie restriction (CR) enhances animal lifespan and prevents age-related diseases, including neurological decline. Recent evidence suggests a mechanism involved in CR-induced lifespan extension is NO●-stimulated mitochondrial biogenesis. We examine here the effects of CR on brain mitochondrial content. CR increased eNOS and nNOS and the content of mitochondrial proteins in the brain. We established an in vitro system to study the neurological effects of CR using serum extracted from animals on this diet. In cultured neurons, CR serum enhanced nNOS expression and increased nitrite levels (a NO● product). CR serum also enhanced the levels of cytochrome c oxidase and increased citrate synthase activity and respiratory rates. CR serum effects were inhibited by L-NAME and mimicked by the NO● donor SNAP. Furthermore, both CR sera and SNAP were capable of improving neuronal survival. Since eNOS is the main source of NO● involved in mitochondrial biogenesis, we investigated the mechanism of NOS activation by treating vascular cells with serum from CR rats and found increased Akt and eNOS phosphorylation, in addition to enhanced nitrite release. Inhibiting Akt phosphorylation or immunoprecipitating adiponectin (found in high quantities in CR serum) completely prevented the increment in nitrite release and eNOS activation. Overall, we demonstrate that adiponectin in the serum from CR animals increases NO• signaling by activating the insulin pathway, resulting in enhanced mitochondrial biogenesis and neuronal survival. Supported by FAPESP, CNPq and INCT/NAP Redoxoma 74 Symp#23 Cytotoxicity -Brazilian-Slovenian Meeting Chairs Sandra Azevedo and Tamara Lah Turnsec Tamara Lah Turnsec Department of Genetic Toxicology and Cancer Biology, National Institute of Biology, Ljubljana, Slovenia. Cancer initiation and promotion is caused by a number of substances of artificial as well as natural origin that perturb normal cell metabolism and in the first case also effect gene structure and expression by direct or indirect effects on cell nuclei. Genetic toxicology is the field that describes ad study such events and when these are initiated by environmentally released substances, the ecotoxicology may explain its effect is origin, distribution and metabolism as well as the effects on different organisms, including humans. In this session all these are combined, leading us from ecotoxicology, associated with water organisms that release a number of toxins into their environment. Being of structurally very diverse families and sizes (from small peptides of unusual structure, that would not be degraded by host proteases (but rather inhibit them) to larger oligomeric protein structures forming pores in the cell membranes. These toxic substances may have differential effects, both on the same and other species in their environment. After overcoming the cell defence and resistance mechanisms, the toxins either cause autophagy, senescence or apoptosis via a number of pathways, which may lead either to cell death or transformation, which primed the cells with the potency to tumour progression. By the same token, a number of toxins are also being testing for their effect on eradicating cancer cells and recently also cancer stem cells. The problem here, especially in the case of cancer stem cells. The latter in particular have developed a number of resistance mechanisms. This being overcome by an appropriate combination of toxins with other strategies to specifically attack cancer cells, offer new strategy in cancer treatment that may lead to improved efects at least in certain cancer strategies. Also, as cancer tissue is composed of a variety of tumour and normal - stromal cells, the effects on these, as well as on their interactions need to be considered in the future. Cyclic cyanopeptides influence cytoskeleton organization in glial cells Bojan Sedmak National Institute of Biology, Ljubljana, Department of Genetic Toxicology and Cancer Biology, Večna pot 111, 1000 Ljubljana, Slovenia, EU Three basic ways of interaction are possible after cell exposure to biologically active substances; interaction with membrane receptors, membrane insertion and cell entrance. The acute toxicity and cytotoxicity of the best known cyanopeptide microcystin (MC) is primarily due to its ability to enter mammalian cells misusing the organic anion-transporting polypeptide system. Strong protein phosphatase inhibitory activity is believed to be the mechanism by which MC destroys liver cells and consecutively the target organ. In terms of genotoxicity brain cells suffer the most damage implying the possibility of MC passage through the blood brain barrier. In addition to MCs bloom forming cyanobacteria produce a variety of other non-hepatotoxic cyclic cyanopeptides similar in origin structure and activity in considerable amount. Our experiments are focused to normal NHA and tumour derived U87 astrocytes to introduce cyanopeptides as research tools and feasible lead substances in pharmacology. We have monitored the influence of cyclic cyanopeptides on both morphological and genetic level. The effects on cell morphology were studied by pursuing the changes in intermediate filament (IF) organization. The target were two IF’s, glial fibrillary protein expressed in many astrocyte cell lines and nestin expressed by many cell types during development and does not persist into adulthood. Epifluorescent and confocal microscopy were used to pursue the morphological changes while the expression of genes controlling the intracellular scaffolding’s biogenesis, organization, polymerization and depolymerization of MF, MT, IF as well as the cytoskeletal regulatory genes, relevant ARF and RHO G-protein members and their regulatory factors was assessed with RT2ProfilerTMPCR Array. Equinatoxin effects on cellular membranes 1 Miša Mojca Cajnko, 1 Maja Marušič, 2 Biserka Bakrač, 1 Simon Caserman, 1,2 Gregor Anderluh 1 National Institute of Chemistry, Ljubljana, Slovenia 2 Department of Biology, Biotechnical Faculty, University of Ljubljana, Slovenia Disruption of cellular membranes is a very efficient way to alter cellular function and, hence, pore-forming toxins are one of the most important groups of natural toxins. The most studied pore forming toxins are bacterial virulence factors. Actinoporins are efficient pore-forming toxins produced by sea anemones. They exclusively form pores in membranes that contain sphingomyelin. They are an important example of so-called alpha-helical pore-forming toxins, since the final conductive pathway is formed by amphipatic alphahelices. The pore formation is a multistep process that involves recognition of the membrane sphingomyelin, firm binding to the membrane accompanied with the transfer of the N-terminal region to the lipid-water interface and final pore formation after oligomerization of several monomers. We have recently shown that equinatoxin II (EqtII), the most studied representative of actinoporins, specifically binds SM, but not other lipids, and described molecular mechanism of SM recognition. By using EqtII as a molecular probe we show that sphingomyelin in the Golgi apparatus is exposed to the cytosol of the cell. Due to its unique features, EqtII was also used to study sphingomyelin distribution in the plasma membrane. EqtII binding was accompanied by extensive plasma membrane reorganization into microscopic domains that resemble coalesced lipid rafts. Pore formation enabled entry of calcium ions in the cell, which was followed in number of responses such as hydrolysis of phosphatidylinositol 4,5-bisphosphate, plasma membrane blebbing, actin cytoskeleton reorganization, and inhibition of endocytosis. In Caco2 epithelial cells EqtII was found to decrease transepithelial electrical resistance. It seems that plasma membrane reorganisation is, at least in part, a killing strategy of actinoporins. 75 Symp#24 Cancer Chair Renata Pasqualini Integration of in Vivo Phage Display & Targeted nanotechnology and Molecular-genetic Imaging Renata Pasqualini, Ph.D. The University of Texas, M D Anderson Cancer Center, USA Our group has previously reported the design, generation, and construction of AAV/phage (termed AAVP) particles (Hajitou et al. 2006, Hajitou et al. 2007, Soghomonyan et al. 2007) for targeted molecular-genetic imaging. These hybrid vectors containing prokaryotic and eukaryotic cis-genomic elements have the potential to integrate ligand-directed targeting and molecular-genetic imaging. In a related line of research, we have used labeled, targeted peptide motifs themselves as imaging tools (Yao et al. 2005, Marchiò et al. 2004, Arap et al. 2004, Zurita et al. 2004, Cardó-Vila et al. 2003, Chen et al. 2003, Mintz et al. 2003). In pilot experiments, AAVP-based molecular-genetic imaging appears to be superior in side-by-side comparison to standard imaging because it provides prediction of therapeutic response in addition to only monitoring (Hajitou et al., PNAS, 2008). Thus, we plan to focus primarily on the development of AAVP-based molecular-genetic imaging approaches. Finally, we have also designed and developed nanotechnology-based (i.e., bottom-up self-assembled) biocompatible networks of phage-gold as nano-molecular sensors and reporters (Souza et al. 2006a, Souza et al. 2006b). This new methodology will be incorporated and will likely prove to be quite synergistic with AAVP (Souza et al. 2011). Here we used prototypes of this new class of targeted hybrid vectors for therapy and for molecular-genetic imaging, in conjunction to the discovery of new ligand motifs that target human tumor endothelium. AAVP-based anti-vascular cancer therapy by targeted TNF in pet dogs with native tumors has also been successful (Paoloni et al. 2009). Ultimately to generate an “imaging transcriptome” for human tumors. The incorporation of transcriptional targeting (through tissue-specific or radiation-induced promoters) to ligand-directed AAVP-targeting may enable one to determine a gene (or set of genes) status without tissue biopsy. Tumor Cell to Tumor Cell Interaction Drives Cancer Heterogeneity Webster K. Cavenee Ludwig Institute for Cancer Research, University of California San Diego, 9500 Gilman Drive, La Jolla, CA 92093-0660 USA Most efforts to understand the consequences of large-scale genomic mining of data from human tumors have focused on their cellintrinsic activities both in vitro and in vivo. Because of this, targeted therapeutic approaches have primarily been directed at features of individual tumor cells and their intrinsic mutations. For example, we have for more than a decade functionally dissected the amplification and mutation of the epidermal growth factor receptor gene (EGFR), that results in the common and oncogenic EGFRvIII (ΔEGFR) variant, a signature pathogenetic event in glioblastoma, the most common intracranial tumor. These analyses have allowed us to develop both small molecule- and antibody-based therapeutics that are now in clinical trials. Paradoxically, despite its greater intrinsic biological activity than wildtype EGFR (wtEGFR), only a minority of cancer cells in primary tumors possesses the hallmark ΔEGFR lesion, while the remainder expresses wtEGFR. We hypothesized that the ΔEGFR-expressing subpopulation has an extrinsic activity that provides enhanced tumorigenicity to the entire tumor cell population, perhaps through a paracrine mechanism. Using a combination of mixed tumor engraftments and biochemical analysis of paracrine factors and signaling pathways activation, we determined that human glioma tissues, glioma cell lines, glioma stem cells and primary mouse astrocytes, that express ΔEGFR each secrete IL-6 and/or LIF cytokines. This then prompts a novel interaction between the receptor that is common to these cytokines, gp130, and wtEGFR in neighboring cells that express amplified levels of EGFR, resulting in co-receptor activation and tumor growth enhancement. Ablating IL-6, LIF or gp130 uncouples this cellular cross-talk and potently attenuates tumor growth enhancement. These findings demonstrate that the heterogeneity that characterizes GBM, and perhaps other tumors with this feature, does not occur stochastically. Instead, it results from both intrinsic and extrinsic activities of driver mutations and can be an actively maintained feature. This illuminates for the first time a heterotypic cancer cell interaction of potential therapeutic significance. Targeting Adipose Tissue to Prevent Cancer Progression Wadih Arap, M.D., Ph.D. The University of Texas, M D Anderson Cancer Center, USA Human obesity is a leading cause of morbidity and mortality and a financial burden worldwide. Despite efforts in past decades, very few drugs have been developed for the treatment of obese patients. The paradigm of obesity treatment currently relies on CNS and/or peripheral metabolic mechanisms to suppress appetite and elevate energy expenditure, or inhibition of fat absorption. Only two Food FDA-approved drugs for weight loss are currently available in the United States (phentermine and orlistat); most unfortunately, placebo-subtracted weight losses are small and concerns over side effects limit their use, hence the great therapeutic challenge. Here we evaluated and validated a new conceptual approach against obesity: targeted induction of apoptosis in blood vessels supplying white adipose tissue (WAT). Our group is a pioneer in this area and has previously designed and has recently established adipotide as a prototype in a new class of drugs that target the vascular endothelium of white fat in pre-clinical models of obese rodents and obese non-human primates. We have chosen to pursue a pilot application of adipotide as a strategy to overcome the obesity-related tumor-promoting effects of obesity in the context of human prostate cancer progression and recurrence. Notably, we have received “safe-to-proceed status” from the FDA for the IND application of adipotide; as such, the start of the firstin-human clinical trial in obese prostate cancer patients is imminent. Our Specific Aims are: (i) To define the metabolic and oncologic consequences of targeted treatment with adipotide in obese men with prostate cancer. (ii) To lead optimize adipotide derivatives and dose-limiting toxicity in rodents and non-human primates. In the short-term, imaging guided studies will enable the rapid translation and drug lead optimization of adipotide and will provide the clinical foundation for approval of an entirely new approach against human obesity. In the long-term, a successful innovative therapy such as adipotide against human obesity would truly be transformative with immense public health impact against not only obesity but also against associated patient co-morbidities including diabetes, metabolic syndrome, arterial hypertension, atherosclerosis, and cancer. 76 Symp#25 Cell motility Chair James Sellers A Tale of Two Tails: The Regulation of Myosin-5a and Myosin-7a James R. Sellers National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD 20892 USA Myosins are a superfamily of molecular motors that can be subdivided into more than 35 classes. Myosins have undergone structural and functional adaptations to perform many duties inside cells and in many cases the activity of the myosin is tightly regulated. In this talk I will discuss the structure, function and regulation of two myosins, mouse myosin-5a and Drosophila myosin-7a. Myosin-5a is a processive cargo transporter in cells which helps move organelles from one point to another, whereas myosin-7a appears to be active in areas of very high actin density such as stereocelia and other actin bundles. In humans, mutations in myosin-7a lead to deafness and blindness. The enzymatic activities of both of these myosins are regulated in vitro through head-tail interactions, but the molecular details of these interactions are very different. Myosin-5a is a dimeric motor and the presence of both heads is important to the regulation, whereas myosin-7a is monomeric. Each myosin has at least one binding partner that is sufficient to activate the myosin from its off state. For myosin-5a this binding partner is termed melanophilin which also interacts with Rab27a to form a tripartite complex that connects the melanosome to actin filaments. We discovered a novel protein in Drosophila that interacts with myosin-7a to activate its activity. The details of the regulation of these two myosins will be discussed. Single Molecule Fluorescence and Optical Trapping Applied to Molecular Motors: Two can do it better than one. Paul Selvin Mindy Tonks Hoffman, Ben H. Blehm, Paul R. Selvin, University of Illinois, Urbana-Champaign Kinesin and dynein are molecular motors that move in opposite directions on a microtubule. They often act on the same cargo, causing the cargo to frequently switch direction. Whether this back-and-forth motion results from a coordinating complex or from a tug-of-war between the two motors is currently unknown. We have applied single molecule fluorescence to determine that they are undergoing a synergistic tug-of-war. By synergistic, we mean that the combination of the two motors is able to bypass roadblocks along the microtubule. Furthermore, using an in vivo optical trap, and by comparing directional stall forces in vivo and in vitro, we found when cargo is going in the positive microtubule direction, kinesin and dynein are pulling, with the dynein walking backwards. The net stall force equals the stall force of kinesin (≈ 7 pN) minus the stall forces of the number of dyneins (1.1 pN x ND, where ND, = 0 to 6). When moving in the negative microtubule direction, the stall force is just equal to a multiple of dynein’s stall force (1.1 pN x ND), implying that kinesin has fallen off the microtubule. Microfluidics pushes forward microscopy analysis of actin dynamics 1 1 1 2 Marie-France Carlier , Antoine Jégou , Guillaume Romet-Lemonne , Thomas Niedermayer and Reinhard Lipowsky 1 Cytoskeleton Dynamics and Motility group, CNRS UPR 3280, 91198 Gif-sur-Yvette France 2 Theory and Biosystems, Max Planck Institute of Colloids and Interfaces, Potsdam Germany 2 Cycles of site-directed actin polymerization and depolymerization, associated with ATP hydolysis, drive a large number of motile processes in eukaryotic cells. The study of actin dynamics and its control by regulatory proteins has mainly relied, for 30 years, on bulk solution kinetic measurements in which the behavior of many filaments is averaged. Recently, individual filament dynamics have been approached using Total Internal Reflection Fluorescence (TIRF) microscopy. The latter method uniquely allows analysis of fluctuations in length of filaments or their processive assembly by formins, but suffers from artifacts resulting from the need to immobilize filaments on a coverslip, the lack of spatial and temporal resolution and tedious image analysis. To overcome these problems, we have implemented microfluidics in the TIRF method. Two issues have been addressed at the scale of single filaments. First, by switching rapidly filaments from polymerizing to depolymerizing conditions, analysis of nucleotide dependent disassembly rate demonstrates that the slow release of Pi following rapid cleavage of ATP on a single filament assembling from ATP-Gactin occurs via a random mechanism. Second, we show that the reported abrupt switches to very slow filament depolymerization, attributed to the structural stabilization of filaments upon ageing (Kue and Mitchison, PNAS 2008, Science 2009) are actual pauses in depolymerization, caused by stochastic formation of photo-induced, immunodetectable covalent actin dimers within the filaments. Statistic analysis of the frequency of pauses shows that pauses represent the slow dissociation of actin dimers. Further developments of microfluidics in the study of actin dynamics will be discussed. 77 Symp#26 RNA regulation - Canadian Society for Cell Biology Chairs and speakers Jean Pierre Perrault and Carla Columbano Impact of G-quadruplex structures on the human transcriptome Jean-Pierre Perreault and Jean-Denis Beaudoin Groupe ARN/RNA group, Département de biochimie, Faculté de médecine et des sciences de la santé, Université de Sherbrooke, Sherbrooke, QC, J1H 5N4, Canada ([email protected]) Given that greater than 90% of the human genome is expressed, it is logical to assume that post-transcriptional regulatory mechanisms must be the primary means of controlling the flow of information from mRNA to protein. Guanine-rich nucleic acid sequences can fold into non-canonical, four stranded helical structures called G-quadruplexes. Initially, we have developped a robust approach that includes in silico, in vitro and in cellulo experiments permitting an in-depth evaluation of the global impact of Gquadruplexes as translational repressors. Briefly, sequences including potential G-quadruplexes were selected within 9 distinct genes encoding proteins involved in various biological processes. Six of these sequences were observed to fold into G-quadruplex structures in vitro, all of which exhibited translational inhibition in cellulo when linked to a reporter gene. In addition, the impact of single nucleotide polymorphism was shown to be important in the formation of G-quadruplexes located within the 5’-untranslated region of an mRNA. Subsequently, the same approach was applied in order to study to evaluate the presence of G-quadruplex structures within human 3'-UTRs. Specifically, two potential G-quadruplex sequences located in the 3'-UTR of the low density lipoprotein receptorrelated protein 5 (LRP5) gene and the fragile X mental retardation autosomal homolog 1 (FXR1) gene were chaarcterized. Both of these G-quadruplex structures increases by 2-fold the gene expression of a reporter gene by stimulating the polyadenylation of its mRNA throughout an alternative site located downstream of the canonical site of their corresponding 3'-UTR. Sequence analysis, site directed mutagenesis, miRNA regulation network analysis and G-quadruplex ligand experiments were performed to define rules governing this phenomenon. In light of these results, we suggest that 3'-UTR G-quadruplexes can regulate alternative polyadenylation sites, leading to the expression of shorter transcripts, and can interfer with the miRNA regulatory network of a specific mRNA. In light of these results, the G-quadruplexes represent a class of RNA motif that is broadly distributed in the cellular transcriptome and have important impact on mRNA species. Identification of proteins regulating the RNA exosome Carla Columbano Oliveira Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo In eukaryotes, many posttranscriptional processing events are necessary for the synthesis of mature RNAs. mRNAs, tRNAs, rRNAs, snRNAs and snoRNAs are processed through several steps that involve specific reactions between RNA and proteins. In fact, most cellular RNAs are associated with proteins, forming ribonucleoprotein complexes (RNPs), which participate in different aspects of gene expression. The main focus of our laboratory has been the study of the posttranscriptional control of gene expression through the functional and structural characterization of proteins that regulate processing of different types of RNA. We will show the identification and functional characterization of Saccharomyces cerevisiae proteins that interact with and regulate the RNA exosome, a protein complex involved in processing and degradation of all types of RNA. Nop53p and Nop8p are nucleolar proteins involved in the maturation of the large ribosomal subunit and regulate the RNA exosome during this process in different ways. While Nop53p activates the exosome, Nop8p inhibits it. These observations led to the hypothesis that the interactions between different proteins and the exosome are responsible for directing the complex to its substrates, and for controlling its activity. Inhibition of RNA Polymerase I as a Strategy to Treat Cancer Megan J. Bywater1, Katherine M. Hannan1, Gretchen Poortinga1, Joanna C. Chan1, Elaine Sanij1, Nadine Hein1, Carleen Cullinane1, Denis Drygin2, William G. Rice2, Ricky W. Johnstone1,3, Grant A. McArthur1,3, Ross D. Hannan1,3 and Richard B. Pearson1,3. 1 Division of Cancer Research, Peter MacCallum Cancer Centre, St Andrews Place, East Melbourne, Vic, Australia; 2Cylene Pharmaceuticals Inc., 5820 Nancy Ridge Drive, San Diego, CA 92121, USA; 3Sir Peter MacCallum Department of Oncology, University of Melbourne, Melbourne Australia. Morphologic abnormalities of the nucleolus, the site of transcription of the ribosomal genes (rDNA) by RNA Polymerase I (Pol I), have been recognized as diagnostic for cancer for more then a century. Furthermore, accelerated ribosome biogenesis is invariably associated with malignant transformation. Nevertheless, a critical, unresolved question has been whether the accelerated ribosome biogenesis responsible for the nucleolar changes is required for maintenance of the malignant phenotype. Here we show that the PI3K/AKT pathway, deregulated in a high proportion of human tumours, is a critical regulator of ribosome biogenesis. Constitutively active AKT is sufficient to drive rRNA synthesis, ribosome biogenesis and cell growth. Furthermore, AKT cooperates with c-MYC to activate rRNA synthesis and ribosome biogenesis identifying the AKT/mTORC1/MYC network as a master controller of cell growth. Consistent with this concept, AKT activity is required for maximal activation of rRNA synthesis and tumour formation in the E-Myc mouse model of Burkitt’s lymphoma (1). Our findings raise the exciting possibility that malignant diseases characterized by unrestrained cellular growth may be vulnerable to therapeutic strategies that target ribosome biogenesis. To directly test this hypothesis, we used genetic manipulation and a novel selective small molecule inhibitor of Pol I transcription (CX5461) (2), to provide the first definitive evidence that accelerated rDNA transcription and nucleolar integrity are necessary for oncogenic activity in hematologic tumour cells. Further, we show that Pol I transcription can be targeted in vivo to therapeutically treat tumors in both genetically engineered and xenograft models of lymphoma and leukemia through the non-genotoxic activation of p53-dependent apoptosis, while sparing normal cells of hematological lineages. Thus, selective inhibition of Pol I transcription, a so called ‘”house keeping” process, can serve as a novel therapeutic strategy for the treatment of cancer (3). (1) Chan J.C, et al (2011) Science Signaling 4 (188), ra56, (2) Drygin, D et al., (2011) Cancer Res, 71(4):1418-3 (3) Bywater, M.J. et al (2012) Cancer Cell (accepted for publication) 78 Symp#27 Maternal interface Chair Estela Bevilacqua Felipe Vadillo-Ortega Universidad Nacional de Mexico (UNAM), Mexico The placenta as an early marker of genomic, proteomic and epigenetic changes involved in vascular diseases Paola Casanello, Krause B, Caniuguir A, Muñoz E, Carrasco I. Faculty of Medicine Pontificia Universidad Católica de Chile Fetal programming resulting from disturbed intrauterine growth induces permanent physiological alterations that increase the risk of developing cardiometabolic diseases in the adulthood. Most of the support for this notion has come from animal models, and correlations between neonatal data and adult health in humans. Interestingly, human placenta seems to represent a good source for the study of this process. There is convincing data showing that macroscopic placental characteristics as well as molecular and epigenetic markers in the placenta at term predict adult cardiometabolic risk. We have studied the effect of hypoxia on vascular function and endothelial physiology in placentae from intrauterine growth restriction (IUGR) fetuses. These studies have shown that endothelial cells from IUGR placentae (IUGR-EC) present altered expression patterns at transcriptional and proteomic levels, similar to those observed in normal EC exposed to hypoxia, confirmed the idea that endothelial dysfunction can be programmed in utero In fact, IUGR-EC present altered expression of eNOS, CAT-1 and arginase-2, which correlate with altered NOSdependent vascular relaxation. Moreover, the altered expression of eNOS in IUGR-EC is associated to specific changes in the DNA methylation status at NOS3 promoter. Interestingly, eNOS expression in IUGR-EC can be reprogrammed preventing the heritance of DNA-methylation patterns by transient silencing of DNMT1. All these data highlight the applicability of placental studies in order to predict future health risk in humans, however further efforts are necessary to validate the implications of these seminal findings and how these could represent the vascular alterations that take place in the fetus. Supported by FONDECYT-1120928, CONICYT Anillos ACT-73, AT24100107(Chile). EM & BK hold CONICYT PhD grants. Expression and function of PSG, StarD7 and KLF6 genes in human trophoblast cells Graciela M Panzetta-Dutari., Racca AC., Camolotto S., Ridano ME., Flores-Martin J., Rena V. & Genti-Raimondi S. CIBICI-CONICET. Departamento de Bioquímica Clínica, Facultad de Ciencias Químicas. Universidad Nacional de Córdoba. Ciudad Universitaria. Córdoba. Argentina. Placenta is intimately related to fetal and maternal health. Villous cytrophoblasts (CTB) differentiate by fusion to form the syncytiotrophoblast (STB) layer characterized by a high metabolic and biosynthetic activity. Human pregnancyspecific glycoproteins (PSG) are the major STB secreted proteins at term, and low PSG levels have been associated with complicated pregnancies. Steroidogenic acute regulatory protein-related lipid transfer domain containing 7 (StarD7) has a wide-spread expression in trophoblastic tissues with highest levels in choriocarcinoma cells, and KLF6 knockout mice exhibit impaired placental development. In order to further elucidate gene expression control and function of these proteins in placental biology, we performed microscopy, qRT-PCR, western-blot, transfection, siRNA, DNAprotein interaction and immunoprecipitation assays, among others, in human trofoblastic cell models. We found that PSGs are early differentiation markers whose expression precedes that of hCG and cell fusion. PSG promoter activation involves regulation by Sp1, KLF6, acetylation/ deacetylation balance and 5`proximal sequences. Remarkably, KLF6 peaks early during the syncytialization process, transactivates PSG and hCG genes, and KLF6 down-regulation inhibits CTB fusion, suggesting it is an essential regulator of trophoblast differentiation. StarD7 expression is regulated by SF-1 and Wnt--catenin signaling which might have important implications in phospholipid uptake and transport contributing to trophoblast development. Finally, as an increased risk of pregnancy alterations has been reported in women chronically exposed to pesticides, we investigated chlorpyrifos effect on trophoblast cells. Exposures to concentrations which did not alter cell viability and fusion modified KLF6, hCG, GCM1, ABCG2, and P-gp but not PSG and StarD7 gene expression. These studies have provided a better understanding about the molecular players involved in trophoblast cell biology and hence in pregnancy maintenance. This study was conducted with the ethics approval from the Human Studies Local Committee. It was supported by CONICET, FONCyT, MinCyT of Córdoba and SECyT-UNC Listing of authors Panzetta-Dutari GM., [email protected]; Racca AC., [email protected]; Camolotto S., [email protected]; Ridano ME., [email protected]; Flores-Martin J., [email protected]; Rena V., [email protected]; Genti-Raimondi S., [email protected] Haya de la Torre y Medina Allende. Ciudad Universitaria. X5000HUA Córdoba, Argentina, 79 Symp #28 Cells as biosensors Chairs Glaucia M Machado Santelli and Paulo Saldiva Cytotoxic indole alkaloids isolated from Duroia macrophylla (Rubiaceae). Cecilia Veronica Nunez1*, Marne Carvalho de Vasconcellos2, Vincent Roumy3, Sevser Sahpaz3, François Bailleul3, Thierry Hennebelle3. 1 Laboratório de Bioprospecção e Biotecnologia, Coordenação de Tecnologia e Inovação, Instituto Nacional de Pesquisas da Amazônia, Aleixo, Manaus, Amazonas, 69060-001, Brazil; 2 Faculdade de Ciências Farmacêuticas, Universidade Federal do Amazonas – UFAM, Rua Alexandre Amorim, 330, Aparecida, Manaus, Amazonas, 69010-330, Brazil; 3 Laboratoire de Pharmacognosie, EA 4481, Université de Lille 2 – Droit e Santé, 59006, Lille, France. Duroia macrophylla Huber is a native plant species of the Amazon region. It is known as cabeça-de-urubú, apuruí or puruí-grande-damata but no medicinal use is described for it. In our research group bioprospection programm, we collected several Rubiaceae plant species, prepare organic and aqueous extracts and assayed to several activities. D. macrophylla extracts were first assayed against Artemia salina, to determine their toxicity. The methanolic leaf extracts was toxic against A. salina, with a LD50 of 40.00 µg/mL. Then, the methanolic leaf extract was fractionated and 4 alkaloids were isolated: two new roxburguine indole alkaloids plus two other known ones. All isolated substances were essayed on tumor cell lines and the new alkaloid 4 showed a cytotoxic activity against HL60 (human leukemia), APC02 (human gastric adenocarcinoma) and B16F10 (murine melanoma) tumor cell lines (IC 50 values of 2.28 µg/mL, 5.08 µg/mL and 5.11 µg/mL, respectively) and a cytotoxicity of 7.8 µg/mL on normal cell line NHI3T3 (fibroblast murine). The alkaloids assayed did not cause membrane disruption in mouse erythrocytes. This is the first chemical study on this species. Our findings showed that Amazonian plant species can contain new active substances, even if they do not have popular use. Acknowledgments: CT-Agro/CNPq, PPBio/CNPq, FAPEAM, INCT - CENBAM/CNPq. Cell-fiber interactions: effects on cell biology Glaucia Maria Machado-Santelli ([email protected]) Department of Cell and Developmental Biology Institute of Biomedical Sciences - University of São Paulo, Brazil Particle toxicology main subject is to understand their cytotoxic and genotoxic mechanisms. Initial studies focused on the evaluation of particles parameters after inhalation such as the diameter, length and biopersistence led to the association of asbestos exposure with several health problems including lung cancer and mesothelioma. The low biopersistence of chrysotile, causing it to disintegrate and become shorter in the lungs, is the main structural features that lead the chrysotile being less pathogenic than the amphiboles. This safety has been controversial since in vitro chrysotile-associated genotoxic potential has been demonstrated. We evaluated the induction of micronucleated, poliploid and multinucleated cells in chrysotile exposed cultured cells. These in vitro studies show that fibers interfere with mitoses and cytokinesis progression leading to multinucleated and polyploid cells. Cell cycle progression is impaired by fibers and multipolar mitosis may be consequence of fiber induced centrosomic amplification. The cell fate was followed by pulse-time microscopy, allowing us to establish how the chrysotile treatment acts on cell cycle progression and its possible relation with other types of fibers. Cellular responses to ambient levels of air pollution Paulo Saldiva ([email protected]) Department of Pathology Faculty of Medicine – University of São Paulo, Brazil The widespread use of fossil fuels has been associated to marked alterations in our environmental. Global climate changes and local air pollutants are known to cause adverse health effects in humans. The use of cells as biosensors of adverse effects have provided valuable information for the process of evaluating environmental risk associated to air pollution. Respiratory epithelium, endothelium, placental trophoblast, cells of seminiferous tubules, endometrium, and cells of reproductive organs of higher plants have been extensively used to detect, quantify and explore the mechanisms of pollution induced injury, disclosing new perspectives to the process of pollution control, aimed to preserve human health. Effects of air contaminants such as endocrine disruption, cardiovascular damage, cancer induction and promotion and persistent inflammation, have been characterized using cellular systems or in vivo toxicological approaches. In this context, biomonitoring of air pollution is nowadays as important as the classical chemical characterization of the concentration of environmental toxics, opening new areas of research in cell biology. 80 Symp#29 MMPs and TIMPs Chairs Ruy Jaeger Membrane Type I- Matrix Metalloproteinase (MMP14): A Multifaceted Cell Surface Protease in Cancer Stanley Zucker Stony Brook University, USA MMP14, an intrinsic plasma membrane proteinase, plays a critical role in digesting basement membrane and extracellular matrices and in inducing cancer cell migration, thereby promoting cancer invasion and metastasis. We and others have demonstrated that MMP14 is highly expressed in most human cancers and correlates with poor clinical outcome. We have evaluated the role of MMP14 in converting quiescent tumor initiating cells (TICs) to metastatic cancer cells. Our studies of the hemopexin (PEX) domain of MMP14 have shown that of the 4 outermost blades, strands I and IV are essential fo cell migration. Peptides mimicking these outermost strands reduced cancer cell migration and angiogenesis in vitro and lung metastasis in vivo. We next examined the effect of cellular hypoxia on MMP14 function in TICs. SK-3rd TICs, isolated by passage of human breast cancer cells in immunodeficient mice, display enhanced lung metastases. Surprisingly, under normoxic conditions, SK-3rd cells displayed minimal increase in cancer invasion. However, when cultured under hypoxic conditions, a dramatic increase in cell invasiveness in 3D collagen gels was demonstrated, which coincided with increased localization of MMP14 at the cell surface. These data suggest that induction of TIC invasion during hypoxia is caused by enhanced trafficking of MMP14 from the trans Golgi network to the plasma membrane. MMP14 also induces the generation of reactive oxygen species in cancer cells, leading to enhanced cancer aggressiveness. Our most recent studies incriminated tumor growth factor-beta as a key intermediary in MMP14 cell signaling in cancer progression. Rama Khokha University Western Ontario, Toronto, Canada Role of matrix metalloproteinases and inflammasome pathway in the development of airway inflammation and fibrosis Vincent Lagente UMR991 INSERM/Université de Rennes 1, Faculté de Pharmacie, 2 avenue du Prof Léon Bernard, 35043 Rennes cedex, France Matrix metalloproteinases (MMPs) are a major group of proteases known to regulate the turn-over of extracellular matrix and so they are suggested to be important in the process of lung disease associated with tissue remodelling. Pulmonary fibrosis has an aggressive course and is usually fatal for an average of three to six years after the onset of symptoms. Pulmonary fibrosis is associated with deposition of extracellular matrix (ECM) components mainly collagen in the lung interstitium. The excessive airway remodeling as a result of an imbalance in the equilibrium of the normal processes of synthesis and degradation of extracellular matrix components could be in favor of anti-protease treatments. We previously demonstrated a significant inhibition of bleomycin-induced pulmonary fibrosis in mice by the MMP inhibitor batimastat. We also reported a correlation of the differences in collagen deposition in the lungs of bleomycin-treated mice with a reduced molar pro-MMP-9/TIMP-1 ratio in broncholaveolar lavage fluid, beginning as early as the inflammatory events at day 1 after bleomycin administration. The differences in TIMP-1 level, particularly at early events after bleomycin administration, suggest that early altered regulation of matrix turnover may be involved in the further development of bleomycin-induced pulmonary fibrosis. We also demonstrated that Inflammasome-NLRP3 pathway associated with the IL-1R/MyD88 signaling is required in the bleomycin-induced increased TIMP-1 level and pulmonary fibrosis in mice. Finally, these observations emphasize those effective therapies for these disorders must be given early in the natural history of the disease, prior to the development of tissue remodeling and fibrosis. 81 Symp #30 Telomeres Chair Maria Isabel Cano Saccharomyces cerevisiae as a model for the study of telomere-mediated replicative senescence Maria Teresa Teixeira Emilie Fallet, Pascale Jolivet, Julien Soudet, Zhou Xu, Kamar Serhal and Maria Teresa Teixeira Institut de Biologie Physico-Chimique, FRE3354 CNRS/UPMC Biologie Moléculaire et Cellulaire des Eucaryotes - 13 rue Pierre et Marie Curie, 75005 Paris, France ; ERC-STG-2010 D-END In the absence of telomere length maintenance, telomeres shorten progressively with every replication cycle due to the DNA-end replication problem. This leads to a permanent cell cycle arrest called replicative senescence. In humans, this process is involved in the aging of certain organs and in suppression of cancer. Telomeres can be re-elongated by telomerase or more rarely by homologous recombination (HR) in cells that proliferate indefinitely such as unicellular eukaryotes, stem cells and cancer cells of multicellular eukaryotes. In telomerase-deficient yeast cells, replicative senescence is defined as an arrest in G2/M after 60-80 generations as telomeres shorten 2-4 nt/cell division. Our analysis of the DNA-end replication problem formally demonstrate that telomere shortening occurs during the synthesis of the leading strand and depends on the length of the 3’-protruding end of chromosomes. Together with the study of factors that regulate the resection and the synthesis of the 5’ strand, our data support a precise molecular model of the DNA replication of telomeres. Senescence in yeast depends on the DNA damage checkpoints, similar to other eukaryotes. A mathematical modeling of the distribution of telomere length and analysis of meiotic products suggests that the shortest telomere in a cell may have a determinant role in the onset of senescence. Accordingly, the DNA damage checkpoints recognize a very short telomere in senescent cells, triggering a replication fork regression and sister chromatid HR. We propose that these pathways counteract the telomere shortening rate allowing a few additional cell divisions before definitive arrest Telomere dysfunction in human disease Rodrigo Calado University of São Paulo, Ribeirão Preto, Brazil Telomeres and telomere repair are basic molecular features of cells possessing linear DNA chromosomes and defects in them result in various diseases. Severe deficiencies result in dyskeratosis congenita, a congenital aplastic anemia with associated mucocutaneous abnormalities. Mutations in TERT, the catalytic component, and TERC, the RNA template, can behave as risk factors for the development of bone marrow failure, pulmonary fibrosis, and hepatic cirrhosis. Both penetrance and organ specificity are variable and not well understood. Chromosome instability is a result of critical shortening of telomeres and cancer. Searching for a CST-like complex at Leishmania spp. telomeres Maria Isabel Cano Instituto de Biociências, Depto. de Genética, UNESP-Botucatu, São Paulo-Brazil, 18618-970, [email protected] In most eukaryotes telomere binding proteins play crucial roles by interacting with several other regulators to ensure proper telomere maintenance and to form high order complexes. The CST complex, mainly formed by RPA-like proteins, is being considered a second telomere capping mode occurring from budding yeast to higher eukaryotes. The role of CST in chromosome-end protection couples the conventional replication machinery and telomere functions and highlights the complexity of the end- protection process. Leishmania spp. telomeres are composed by TTAGGG repeats which are maintained by telomerase. The basic Leishmania telomeric complex is formed by the proteins RPA-1 and Rbp38, which bind in vitro and in vivo, with high affinity to the G-rich telomeric strand, and by the TRF orthologue representing a shelterin component of this protozoan. Using a large scale search on the tri-tryps database we were able to confirm that the Leishmania spp. genome, like other trypanosomatids, lacks all of the conserved telomere-end-binding proteins found in other eukaryotes, such as the key components of the CST (e.g. CDC13 and CTC) and the shelterin (POT1) complexes. Thus, we speculate that the Leishmania RPA-1 homologue may play the same roles as POT1/CDC13 at parasite telomeres. In this report we used different approaches to show that RPA-1 interacts with both Rbp38 and with telomerase. And also that the putative Leishmania CST-like complex meets the TRF orthologue by physical interactions between Rbp38 and TRF. We speculate whether these protein interactions reflect the entire telomeric complex or the presence of functionally distinct subcomplexes at parasite telomeres. Supported by: FAPESP, CNPq 82 Symp#31 Cancer Stemness -Taiwan Cell Biology Society Chair Ken Wu Tariq Enver Stem Cell Laboratory, UCL Cancer Institute, University College London, London, UK Relapses after therapy-induced complete clinical remissions remain the most significant challenge in cancer therapy. This suggests that a proportion of cancer cells at presentation, escape therapy and persist during remission. These cells presumably are the source of relapse. Why are these cells chemoresistant? We argue that the answer lies in a combination of genetic and epigenetic heterogeneity acting to some degree at the level of 'cancer stem' or 'tumour propagating' cells. We have obtained evidence in support of this conceptual framework for cancer resistance in the context or childhood ALL, the commonest cancer of children. Our results encourage a re-positioning of the cancer stem cell concept as it relates to disease in patients. SOX2 promotes lung cancer stemness by inducing EGFR and BCL2L1 expression Cheng-Wen Wu Institute of Biomedical Sciences, Academia Sinica; and Program in Molecular Medicine, National Yang-Ming University, Taipei, Taiwan, ROC. Tumor cells have long been observed to share several biological characteristics with normal stem/progenitor cells; however, the molecular mechanisms eliciting cancer stemness features in tumors remain elusive. SOX2 is a key regulator for maintaining stemness properties in lung progenitor cells. Here we report the discovery and involvement of SOX2 in the development of lung cancer stemness. SOX2 expression was associated with poor prognosis of lung cancer patients. SOX2 was expressed in a subclass of lung cancer cells, the self-renewal and proliferation of which was dependent on SOX2 signaling. SOX2 induced EGFR expression via binding to the EGFR promoter, and EGFR activation further upregulated SOX2 levels, forming a positive feedback loop. SOX2 overexpression promoted chemoresistance, and SOX2 silencing perturbed mitochondrial integrity with marked apoptosis and autophagy. SOX2 induced BCL2L1 expression through binding its promoter. Ectopic BCL2L1 expression rescued SOX2 silencing–induced apoptosis, autophagy, and mitochondrial abnormality. SOX2 overexpression induced tumor formation, and SOX2 knockdown attenuated tumor growth in a xenograft mouse model. SOX2, EGFR and BCL2L1 expression was significantly correlated in primary lung tumors. These data support the critical role of SOX2 in the development of lung cancer stemness via activation of EGFR and BCL2L1 signaling . TBA 83 Symp#32 Unconventional organelles Chair Marlene Benchimol Reductive evolution and the minimal mitochondria of microsporidian parasites Martin Embley Institute for Cell and Molecular Biosciences, The Medical School, Newcastle University, UK NE24HH Microsporidians are important human pathogens causing chronic diarrhoea in children and the elderly, and infecting immunocompromised patients, including those with HIV/AIDS. In addition to their medical importance, microsporidians have become models for understanding cellular and genomic reduction in eukaryotes. The adoption of an obligate intracellular lifestyle has allowed them to lose metabolic pathways and to simplify the structures and functions of cellular organelles. In my talk I will discuss how such reductive evolution has affected the proteome and functions of their minimal mitochondria – now widely referred to as mitosomes, and why, despite their reduced nature mitosomes are still essential for parasite viability. An unconventional organelle: the hydrogenosome Marlene Benchimol Universidade Santa Úrsula, Laboratório de Ultraestrutura Celular - Rio de Janeiro - Brazil Hydrogenosomes are spherical or slightly elongated organelles found in non-mitochondrial organisms. Like mitochondria hydrogenosomes: (1) are surrounded by two closely apposed membranes and present a granular matrix: (2) divide in three different ways: segmentation, partition and the heart form; (3) they may divide at any phase of the cell cycle; (4) produce ATP; (5) participate in the metabolism of pyruvate formed during glycolysis; (6) present a relationship with the endoplasmic reticulum; (7) incorporate calcium; (8) import proteins post-translationally; (9) present cardiolipin. However, there are differences, such as: (1) absence of genetic material, at least in trichomonas; (2) lack a respiratory chain and cytochromes; (3) absence of the F0- F1 ATPase; (4) absence of the tricarboxylic acid cycle; (5) lack of oxidative phosphorylation; (6) presence of peripheral vesicles. Hydrogenosomes are considered an excellent drug target since their metabolic pathway is distinct from those found in mitochondria and thus medicines directed to these organelles will probably not affect the host-cell. The main drug used against trichomonads is metronidazole, although other drugs such as β-Lapachone, colchicine, Taxol, nocodazole, griseofulvin, cytochalasins, hydroxyurea, among others, have been used in trichomonad studies, showing: (1) flagella internalization forming pseudocyst; (2) dysfunctional hydrogenosomes; (3) hydrogenosomes with abnormal sizes and shapes and with an electron dense deposit called nucleoid; (4) intense autophagy in which hydrogenosomes are removed and further digested in lysosomes. Dynamic control of the contractile vacuole complex and acidocalcisomes and their functional role in the mechanisms of regulatory volume decrease in Trypanosomatid parasites Kildare Miranda1 Wendell Girard-Dias1, Wanderley de Souza1 and Roberto Docampo2 , 1Biophysics Institute, Federal University of Rio de Janeiro, 2 University of Georgia Understanding mechanisms involved in osmoregulation control in protozoan parasites has been a challenge for many research groups. Among these mechanisms, a cyclic AMP (cAMP) signaling pathway has been shown to play a key role in osmoregulation, through a mechanism that involves the activation of an unusual organelle named the contractile vacuole complex (CVC). In Trypanosoma cruzi, the CVC is formed by a central vacuole surrounded by interconnected tubules that undergo dynamic changes upon osmotic stress and interacts with acidocalcisomes, whose structural organization, chemical properties and physiological activity may also vary upon events of osmotic stress. Biochemical and molecular data have shown that the sequence of events that take place in cells submitted to hyposmotic stress leads to an increase in cAMP levels, stimulating the traffic of an aquaporin from acidocalcisomes to the CVC through a fusion mechanism. Acidocalcisomes contain basic amino acids and high levels of cations and polyphosphate, a content that once released within the contractile vacuole, leads to an increase in the osmotic pressure towards the lumen of the organelle, stimulating water transport into the CVC. Functional analysis of mutant parasites that overexpress enzymes involved in the control of cAMP levels showed alterations in the regulatory volume decrease (RVD), a large and functional CVC and were more efficient in volume recovery. Taken together, our data show dynamic changes in the osmoregulatory system of T. cruzi, governed by signaling events that involve a unique mechanism of interaction of the CVC with acidocalcisomal components. Cell biology of magnetotactic bacteria and their organelles: the magnetosomes Ulysses Lins Instituto de Microbiologia, UFRJ, Centro de Ciências da Saúde, Bloco I, Avenida Carlos Chagas Filho, 373 - 21941-902, Rio de Janeiro, RJ, Brasil Historically prokaryotes have been described as simple cells with organization principles distantly related to the more complex eukaryotic cells. Recent advances in understanding the cell biology and molecular mechanisms underlying the ultrastructural organization of bacterial cells have modified the traditional views used to distinguish eukaryotic from prokaryotic cells. The complexity of prokaryotic cells and their similarities to eukaryotes is highlighted by the discovery of cytoskeleton elements in bacteria and further by the description of membranous organelles with specialized functions in a number of prokaryotic species. One of these organelles, the magnetosome, consists of a nanometer-sized magnetic crystal which is formed in the bacterial cell cytoplasm inside a bilayer lipid vesicle containing a unique set of proteins. The magnetosomes are organized as chains within the cell and are surrounded by a distinct cytoskeletal network of filaments. Bacteria that produce magnetosomes are called magnetotactic bacteria because of their ability to orientate and navigate along magnetic field lines which is a consequence of the magnetic moment generated by the chains of magnetosomes. The specific proteins expressed by the cell in the magnetosome membrane modulate the biomineralization of the magnetic crystals within the magnetosome vesicle. Consequently, the controlled biomineralization process that takes place in magnetosomes produce magnetic crystals with unique morphologies that is dependent on the magnetotactic bacterial species. 84 Poster Sessions July 26th (Thursday) Cell Biology Cell Biology and Inflammation Cell Biology in Education Cell Cycle and Proliferation Cell Differentiation Cell Therapy Cells as Biosensors Cytoskeleton Developmental Biology Epigenetics Gene Therapy Host Parasite Interaction Methods in Cell Biology Plant Cell Biology Plasma Membrane and Organelles Proteolysis Stem Cells Board ID A1-A122 C1-C118 E1-E12 F-1-F27 H1-H19 K1-K16 L1-L7 M1-M13 N1-N51 O1-O18 Q1-Q5 R1-R75 S1-S30 U1-U41 V1-V10 X1-X10 Z1-Z42 Floor 1 1 2 2 1 2 2 2 2 2 2 2 1 2 1 2 2 July 27th (Friday) Cell Biology and Cancer Cell Biology and Reproduction Cell Death Cell Migration Cell Signaling Extracellular Matrix Neurobiology Board ID B1-B252 D1-D140 G1-G39 I1-I13 J1-J48 P1-P37 T1-T85 Floor 1 2 2 2 1 2 2 85 Presentation guidelines Poster Sessions will be held at 1st and 2nd floors (Room 203) and will be organized according to the different areas. Please check your Board ID above. Poster Session I: Thursday, July 26th Set up: Wednesday 15h00- 17h00 and Thursday 9h00- 9h30 Tear down: until 15h30 Poster Session II: Friday, July 27th Set up: Thursday 16h30 -18h00 and Friday 9h00- 9h30 Tear down: 16h00- 16h30 Author presentation hour for both sessions: 11h45- 12h45 even numbers 12h45-13h45- odd numbers Poster numbers will identify the boards. Tapes and hangers should be brought to the area by presenters. The Organizing Committee will not provide these items and will not collect and keep Posters that are left on the Boards. We will invite our speakers to select and nominate three best posters according to their expertise. Winners and prizes will be announced during the closing cerimony 86 A – Cell Biology A1-A122 A-1 EARLY WEANING AFFECTS CORTICOSTERONE LEVELS, CBG BINDING CAPACITY AND GLUCOCORTICOID RECEPTOR IN THE GASTRIC MUCOSA OF RATS DURING POSTNATAL DEVELOPMENT. HELOISA GHIZONI, PRISCILA MOREIRA FIGUEIREDO, MARIE-PIERRE MOISAN, LUCIANA HARUMI OSAKI, CRUZ ALBERTO MENDOZA RIGONATI, PATRÍCIA GAMA A-2 NORMAL AND REGENERATION BONE TISSUE ANALYSIS: USE OF FOURIER TRANSFORMED SPECTROSCOPY INFRARED (FTIR) FOR IN SITU MOLECULAR IDENTIFICATION. TACIANA D. MAGRINI, HERCULANO S. MARTINHO, ANA AMÉLIA RODRIGUES, NILZA BATISTA, WILLIAM D. BELANGERO, ARNALDO R. SANTOS JR A-3 A NOVEL LEISHMANIA AMAZONENSIS PROTEIN WICH INTERACTS HIGHLY SPECIFICALLY WITH THE G-RICH TELOMERIC STRAND. VINICIUS SANTANA NUNES, MARIBEL FERNÁNDEZ FERNÁNDEZ, CRISTINA BRAGA DE BRITO LIRA, MARIA ISABEL NOGUEIRA CANO A-4 AN EVALUATION OF CHONDROCYTES MORPHOLOGY AND GENE EXPRESSION ON SUPERHYDROPHILIC VERTICALLY-ALIGNED MULTI-WALLED CARBON NANOTUBES FILMS. ELIANE ANTONIOLI, ANDERSON O. LOBO, DANIELLA Z. BUCCI, MARIO FERRETTI, MOISÉS COHEN, EVALDO J. CORAT, VLADIMIR J. TRAVA-AIROLDI A-5 ALTERED EXPRESSION OF LEPTIN AND GHRELIN IN THYMUS OF ALOXAN-INDUCED DIABETIC MICE. CAROLINA FRANCELIN, IEDA GENISELI, LIANA VERINAUD A-6 ANALYSIS OF WNT PATHWAY COMPONENTS IN AN EXPERIMENTAL MODEL OF ENDOMETRIOSIS. RÔMULO MEDINA DE MATTOS, PAULA RODRIGUES PEREIRA, FÁBIO HECHT CASTRO MEDEIROS, DENISE PIRES DE CARVALHO, LUCIANA BUENO FERREIRA, ETEL RODRIGUES PEREIRA GIMBA, FELIPE LEITE DE OLIVEIRA, LEANDRO MIRANDA ALVES, LUIZ EURICO NASCIUTTI A-7 INFLUENCE OF BIOMODULATION IN CELL CULTURE GIRARDIA TIGRINA (PLATYHELMINTHES, TRICLADIDA). KARLA ANDRESSA RUIZ LOPES, ROBERTA CARICATTO BERNARDO PINTO, NÁDIA MARIA RODRIGUES DE CAMPOS VELHO, CRISTINA PACHECO SOARES A-8 ACTION OF SULFATED GALACTANS FROM RED ALGAE HYPNEA MUSCIFORMIS ON HEMOSTASIS, CELL PROLIFERATION AND CYCLE CELL PROGRESSION. MONIQUE GABRIELA DAS CHAGAS FAUSTINO ALVES, CELINA MARIA PINTO GUERRA DORE, KAHENA DE QUEVEDO FLORENTIN, LUIZA S.E.P. WILL, THUANE DE SOUZA PINHEIRO, HUGO ALEXANDRE DE OLIVEIRA ROCHA, EDDA LISBOA LEITE A-9 EFFECT OF RESISTANCE TRAINING ON BLOOD PRESSURE, ARTERIAL MORPHOLOGY AND VEGF PROTEIN EXPRESSION ON L-NAME HYPERTENSIVE RATS. ANNE CAROLLINE VERÍSSIMO DOS SANTOS, AYSLAN JORGE SANTOS DE ARAUJO, KARINE DOS SANTOS SOUZA, MARLÚCIA BASTOS AIRES, EMERSON TICONA FIORETTO, VALTER JOVINIANO DE SANTANA-FILHO, MARCIO ROBERTO VIANA DOS SANTOS A - 10 INTERACTION CELL-CHRYSOTILE IN TWO DIFFERENT CELL LINES: A MORPHOLOGICAL APPROACH. LUANA RIBEIRO RICARDI, BEATRIZ DE ARAUJO CORTEZ, PAULA REZENDE-TEIXEIRA, GLÁUCIA MARIA MACHADO-SANTELLI A - 11 α-A2BP1 MARKS P-BODIES CONTAINING REPRESSOR/DECCAPING MRNP COMPLEXES DEVOID OF GW182 IN DROSOPHILA S2 CELLS. GUSTAVO BORGES PEREIRA, MARIANA SANTOS DE QUEIROZ, DEISE CRISTINA POLETO SCAGLIA, MARIA LUISA PAÇÓ-LARSON A - 12 NITRIC OXIDE PRODUCTION BY ASCIDIAN HEMOCYTES AFTER HEAVY METALS EXPOSURE. DANIELLY DA FONTE CARVALHO MARTINS, LAURA CARRIELLO EMRICH, SILVANA ALLODI, RODRIGO NUNES DA FONSECA, CINTIA MONTEIRO DE BARROS A - 13 GILL NA+/K+-ATPASE ACTIVITY AND HISTOLOGICAL CHANGES OF FISH BATHYGOBIUS SOPORATOR (GOBIIDAE) DURING ACCLIMATION TO DIFFERENT SALINITIES AND TIMES. CLÁUDIO A. PIECHNIK, LUCÉLIA DONATTI, MARIA ROSA D. PEDREIRO, PRISCILA KREBSBACH, HELENA G. KAWALL A - 14 DECREASE OF THE ATAXIN-2 LEVELS SPECIFICALLY IN THE FAT BODY INTERFERES WITH GROWTH AND SURVIVAL OF DROSOPHILA. MURILO CARLOS BIZAM VIANNA, DEISE CRISTINA POLETO, PAULA FERNANDA GOMES, MARIA LUISA PAÇÓ-LARSON A - 15 DROSOPHILA ATAXIN-2 RELOCATES TO STRESS GRANULES IN RESPONSE TO THERMAL OR OXIDATIVE STRESS. PAULA FERNANDA GOMES, DEISE CRISTINA POLETO-SCAGLIA, MARIA LUISA PAÇÓ-LARSON A - 16 ASSESSING THE DIFFERENTIATION OF QUAIL TRUNK NEURAL CREST CELLS ON A 3D ENVIRONMENT.. FLAVIO AUGUSTO ROCHA BARBOSA, ANA RAMOS HRYB, ANDRÉA GONÇALVES TRENTIN, GIORDANO WOSGRAU CALLONI A - 17 INFLUENCE OF THE VITAMIN C AND HESPERIDIN ON THE EFFECTS OF EXCESSIVE SUCROSE INTAKE IN RATS: A STUDY ABOUT BLOOD GLUCOSE, MEMORY, AND DNA DAMAGE IN BLOOD AND HIPPOCAMPAL CELLS. CAMILA MAI, PATRÍCIA MOLZ, FERNANDA FLEIG ZENKNER, DEIVIS DE CAMPOS, JOEL HENRIQUE ELLWANGER, PAULA FENGLER, LUIZA MÜLLER, DANIEL PRÁ, SILVIA ISABEL RECH FRANKE A - 18 SPERM MORPHOLOGY IN MICRATHYRIA HESPERIS RIS, 1911 (ODONATA, ANISOPTERA). ANA PAULA DE ALMEIDA CAIXEIRO, LUIZ FERNANDO GOMES, CLÁUDIA VÂNIA MIRANDA DE OLIVEIRA, JOSÉ LINO-NETO A - 19 MORPHOLOGY OF THE PERICARDIAL NEPHROCYTES IN TERMITES (INSECTA, ISOPTERA). ANA MARIA COSTA LEONARDO, LARA TEIXEIRA LARANJO, VANELIZE JANEI, IVES HAIFIG A - 20 PRESENILIN 2 REGULATES THE DEGRADATION OF RBP-JK PROTEIN VIA P38 MITOGEN-ACTIVATED PROTEIN KINASE: DEGRADATION OF RBP-JK INVOLVES BOTH PROTEASOME AND LYSOSOME. SU-MAN KIM, MI-YEON KIM, EUN-JUNG ANN, JUNG-SOON MO, JI-HYE YOON, HEE-SAE PARK A - 21 LEVELS OF ABSORPTION AND MORPHOLOGICAL ASPECTS IN FETAL BONE OF WISTAR RATS TREATED WITH ACETATE LEAD. IURE CARVALHO DE SOUZA, ANA PATRÍCIA SANTOS DE OLIVEIRA, RICARDO SCHER, WALDECY DE LUCCA JUNIOR, KÁTIA MICHELLE DOS ANJOS BOMFIM, LINCOLN VÍTOR SANTOS, VÍCTOR SANTANA SANTOS, JOSÉ ARNALDO VASCONCELOS PALMEIRA, FRANCISCO PRADO REIS, CARLOS ALEXANDRE BORGES GARCIA, VERA LÚCIA CORRÊA FEITOSA A - 22 LEVELS OF ABSORPTION AND MORPHOLOGICAL ASPECTS IN FETAL SKIN OF WISTAR RATS TREATED WITH LEAD ACETATE. ANA PATRÍCIA SANTOS DE OLIVEIRA, IURE CARVALHO DE SOUZA, RICARDO SCHER, WALDECY DE LUCCA JUNIOR, CARLOS ALEXANDRE BORGES GARCIA, KÁTIA MICHELLE DOS ANJOS BOMFIM, LINCOLN VÍTOR SANTOS, VÍCTOR SANTANA SANTOS, JOSÉ ARNALDO VASCONCELOS PALMEIRA, FRANCISCO PRADO REIS, VERA LÚCIA CORRÊA FEITOSA A - 23 MONOMERIC RECOMBINANT ARTINM ACTIVATES MAST CELLS AND BINDS TO CALRETICULIN ON THE MAST CELL SURFACE. VALÉRIA CINTRA BARBOSA LORENZI, MARIA CRISTINA ROQUE ANTUNES BARREIRA, MARIA CÉLILA JAMUR, CONSTANCE OLIVER A - 24 GANGLIOSIDE DEFICIENT MAST CELLS DO NOT EXPRESS GALECTIN1. VIVIAN MARINO MAZUCATO, ADRIANA MARIA MARIANO SILVEIRA E SOUZA, MARCELA GIMENEZ, JOSE CESAR ROSA, LUIS LAMBERTI PINTO DA SILVA, MARIA CELIA JAMUR, CONSTANCE OLIVER A - 25 A NEW PHOSPHORYLATION SITE IN ALPHA-TUBULIN SUGGESTS THAT PHOSPHORYLATION MAY BE IMPORTANT TO STABILIZE MICROTUBULES DURING CELL DIVISION. MARIANA LEMOS DUARTE, MUNIRA MUHAMMAD ABDEL BAQUI, HELIO MIRANDA COSTA-JUNIOR, DENISE APARECIDA BERTI, JULIO CESAR BATISTA FERREIRA, TIAGO JOSÉ PASCHOAL SOBREIRA, MARIE-HÉLÈNE DISATNIK, DARIA MOCHLY-ROSEN, PAULO SÉRGIO LOPES DE OLIVEIRA, DEBORAH SCHECHTMAN A - 26 IDENTIFICATION AND CHARACTERIZATION OF THE INTERACTION BETWEEN THE KINASE CELL CYCLE REGULATOR KIS AND THE PROLIFERATION MARKER CATS. ISABELLA BARBUTTI GONÇALVES, JOÃO AGOSTINHO MACHADONETO, ADRIANA S. S. DUARTE, FERNANDA SOARES NIEMANN, SARA TERESINHA OLALLA SAAD, LETICIA FRÖHLICH ARCHANGELO A - 27 A2BP1 IS PRESENT IN MRNP GRANULES CONTAINING THE RNA HELICASE ME31B IN DROSOPHILA EYE DISC AND OVARIAN NURSE CELLS. MARIANA SANTOS DE QUEIROZ, MAYARA TERRA VILLELA VIEIRA, GUSTAVO BORGES PEREIRA, DEISE CRISTINA POLETO SCAGLIA, MARIA LUISA PAÇÓ LARSON A - 28 GALECTIN-3 IS IMPORTANT FOR MAST CELLS MIGRATION. VANINA DANUZA TOSO, MARIA RITA DE CÁSSIA CAMPOS, DEVANDIR ANTÔNIO DE SOUZA JÚNIOR, MARCELO DIAS BARIFFI, MARIA CRISTINA ROQUE ANTUNES BARREIRA, CONSTANCE OLIVER, MARIA CÉLIA JAMUR A - 29 ANALYSIS OF INTRACELLULAR TRAFFIC MEDIATED BY RAB PROTEINS IN HIPPOCAMPUS BEFORE PROTEIN AGGREGATION RELATED TO NEURODEGENERATION. THAIANY QUEVEDO MELO, MERARI F. R. FERRARI A - 30 COMBINED ACTION OF THE GROWTH HORMONE AND INSULINLIKE GROWTH FACTOR-1 ON THYMOCYTES IN VITRO. MARVIN PAULO LINS, IANA MAYANE MENDES NICÁCIO VIANA, LARISSA FERNANDA ARAÚJO VIEIRA, SALETE SMANIOTTO A - 31 AEROBIC TRAINING ENHANCES THE REGENERATION PROCESS AFTER MUSCLE ATROPHIC STIMULUS. RAQUEL SANTILONE BERTAGLIA, IVAN JOSÉ VECHETTI-JUNIOR, PAULO HENRIQUE DO PRADO, HENRIQUE BORGATTO DE ALMEIDA DIAS, ROBSON FRANCISCO CARVALHO, MAELI DAL PAI SILVA A - 32 CYTOTOXICITY IN PRE-DIABETES. PATRÍCIA MOLZ, CAMILA SCHREINER PEREIRA, MORGANA TONET MENDONÇA, THIAGO ALEY BRITES DE FREITAS, SHARBEL WEIDNER MALUF, JORGE ANDRÉ HORTA, DANIEL PRÁ, SILVIA ISABEL RECH FRANKE 87 A - 33 MECHANISMS INVOLVED IN THE GASTROPROTECTION INDUCED BY EUGENIA DYSENTERICA DC (MYRTACEAE) LEAF EXTRACT IN MICE. LIGIA CAROLINA DA SILVA PRADO, ANGÉLICA MARTINS MOREIRA MUNDIM, CAMILA RODRIGUES FERRAZ, HUDSON ARMANDO NUNES CANABRAVA, LUIZ BORGES BISPO-DA-SILVA A - 34 UNDERSTANDING HEPARAN SULFATE /HEPARIN BIOSYNTHESIS. CARINA MUCCIOLO MELO, MARIA APARECIDA PINHAL A - 35 STUDYING THE INTERACTIONS BETWEEN LEISHMANIA AMAZONENSIS TTAGGG REPEAT BINDING FACTOR (LATRF) AND ITS POSSIBLE PARTNERS. JOÃO AUGUSTO RIBEIRO, DOUGLAS DIEZ GONÇALVES, ARINA MARINA PEREZ, PAULO VINÍCIUS DA MATA MADEIRA, MARCELO SANTOS DA SILVA, MARIA ISABEL NOGUEIRA CANO A - 51 MORPHOLOGICAL CHARACTERIZATION OF THE EPIDERMIS OF THE NEOTROPICAL CATFISH PIMELODELLA CF. LATERISTRIGA LICHTENSTEIN, 1823 (OSTARIOPHYSI: SILURIFORMES). KARINA MANCINI, EDUARDO MEDEIROS DAMASCENO, LUIZ FERNANDO DUBOC, JULIANA CASTRO MONTEIRO A - 52 EFFECTS OF YERBA MATÉ (ILEX PARAGUARIENSIS) ON ADIPOSE TISSUE OF RATS PROGRAMMED BY EARLY WEANING. NATÁLIA DA SILVA LIMA, ANA PAULA SANTOS DA SILVA DE OLIVEIRA, VANESSA S. TAVARES RODRIGUES, ANDREA KAEZER, EGBERTO GASPAR DE MOURA, ELAINE DE OLIVEIRA, PATRICIA CRISTINA LISBOA A - 53 ENTAMOEBA HISTOLYTICA IS ABLE TO INTERNALIZE AND DEGRADE TRYPOMASTIGOTES OF T. CRUZI G AND CL STRAINS. FLÁVIA ALVES MARTINS, ADELE AUD RODRIGUES, MARIA APARECIDA GOMES, CLAUDIO VIEIRA DA SILVA A - 36 IDENTIFICATION OF CANDIDATE TRANSCRIPTION FACTORS FOR THE REGULATION OF RAT VENTRAL PROSTATE GLAND RESPONSE TO ANDROGEN DEPRIVATION AND HIGH DOSE 17BETA-ESTRADIOL ADMINISTRATION. RAFAELA DA ROSA RIBEIRO, RAMON OLIVEIRA VIDAL, HERNADES F. CARVALHO A - 54 AGE EFFECTS ON CHROMATIN SUPRA-ORGANIZATION OF CORTICAL NEURONS FROM MICE. HENRIQUE FERREIRA RODRIGUES, TAFAREL ANDRADE DE SOUZA, FLAVIA GERELLI GHIRALDINI, MARCELO EMILIO BELETTI, MARIA LUIZA SILVEIRA MELLO, ALBERTO DA SILVA MORAES A - 37 AEROBIC TRAINING HAS ANTI ATROPHY BENEFICIAL AND CARDIAC REMODELING EFFECTS IN RATS WITH HEART FAILURE. WARLEN PEREIRA PIEDADE, RODRIGO WAGNER ALVES DE SOUZA, LUANA CAMPOS SOARES, DIJON HENRIQUE SALOMÉ CAMPOS, PAULA AIELLO TOMÉ DE SOUZA, ANTONIO CARLOS CICOGNA, MAELI DAL-PAI-SILVA A - 55 EXPRESSION AND FUNCTION OF PSG, STARD7 AND KLF6 GENES IN HUMAN TROPHOBLAST CELLS. PANZETTA-DUTARI GM, RACCA AC., CAMOLOTTO S., RIDANO ME., FLORES-MARTIN J., RENA V., GENTI-RAIMONDI S A - 38 AMPHOTERICIN B INDUCES APOPTOSIS ON A CELL LINE OF HEPATIC STELLATE CELLS. CAROLINA URIBE CRUZ, FERNANDA OLIVEIRA DOS SANTOS, NELSON ALEXANDDRE KRETZMANN, THEMIS REVERBEL DA SILVEIRA, URSULA MATTE A - 39 EFFECT OF POSACONAZOLE ON THREE-DIMENSIONAL CARDIOMYOCYTES CULTURE DURING TRYPANOSOMA CRUZI INFECTION WITH EMPHASIS IN EXTRACELLULAR MATRIX PROTEINS AND GAP JUNCTIONS. LÍNDICE MITIE NISIMURA, PATRÍCIA MELLO FERRÃO, LAURA LACERDA COELHO, LUCIANA RIBEIRO GARZONI A - 40 LUEHEA INFUSION OINTMENT IMPROVES WOUNDED EPIDERMAL TISSUE HEALING IN WISTAR RATS. PAULO CÉSAR FERREIRA DOS SANTOS, TATIANA MORDENTE CLEMENTE, FABRÍCIO CASTRO MACHADO, FERNANDA MIYAGAKI SHOYAMA, CLAUDIO VIEIRA DA SILVA A - 41 LIVER HISTOLOGY OF THE THREE TELEOST SPECIES CAPTURED IN THE ITAPECERICA RIVER, DIVINÓPOLIS, MG, BRAZIL. REGIANNE FERREIRA SILVA, CAMILA FERREIRA SALES, MARILIA GABRIELA C AMARAL, HEDER J. RIBEIRO, ROSY I. MACIEL AZAMBUJA RIBEIRO, FABRICIO FLÁVIO THEOPHILO DOMINGOS, RALPH GRUPPI THOMÉ, HÉLIO BATISTA DOS SANTOS A - 42 MASTER SWITCH REGULATORY GENES INVOLVED WITH PROSTATE GLAND REMODELING AFTER CASTRATION. UMAR NISHAN, DANILO MARCHETE DAMAS DE SOUZA, GUILHERME OLIVEIRA BARBOSA, HERNANDES F. CARVALHO A - 43 PLANTS EXTRACTS AND HUMAN PLATELETS MODULATE MAST CELLS POPULATION IN SKIN WOUND HEALING. LUCIANA XAVIER PEREIRA, RAÍSSA DE OLIVEIRA AQUINO SCHÜFFNER, PATRÍCIA PEREIRA SILVA, HÉLIO BATISTA SANTOS, RALPH GRUPPI THOMÉ, HÉLIO CHIARINI GARCIA, ROSSANA CORREA NETTO DE MELO, ROSY IARA MACIEL DE AZAMBUJA RIBEIRO, GLEYDES GAMBOGI PARREIRA A - 44 TOPICAL ANTIMICROBIAL ACTIVITY OF OLEIC AND LINOLEIC ACIDS IN WOUNDS. MAYSA BRAGA BARROS SILVA, GILSON MASAHIRO MURATA, RUI CURI, ANDREA MARIA SPESSOTO, ELAINE HATANAKA A - 45 FEZ1 PROTEIN-PROTEIN INTERACTION NETWORK GIVING CLUES TO CELLULAR PROCESSES: THE AUTOPHAGY MACHINERY IN NEUROGENESIS AND LEUKEMIA. ARIANE DA SILVA FURLAN, MARCOS RODRIGO ALBORGHETTI, DEIVID LUCAS DOS SANTOSMIGUELETI, JÚLIO CÉSAR SILVA, IRIS CONCEPCION LINARES DE TORRIANI, HOZANA ANDRADE CASTILLO, JOSE XAVIER NETO, JORG KOBARG A - 46 2D-DIGE ANALYSES IN PROTEIN EXPRESSION IN HUMAN UMBILICAL VEIN ENDOTHELIAL CELLS: THE EFFECT OF HYPOXIA IN VITRO AND IN UTERO. ANDRÉS CANIUGUIR, BERNARDO KRAUSE, ERNESTO MUÑOZ, PAOLA CASANELLO A - 47 STREPTOMYCIN EFFECTS IN STRETCH-ACTIVATED CHANNEL PROTEIN TRPC1 LEVELS AND MYONECROSIS OF MDX MICE. CINTIA YURI MATSUMURA, ANA PAULA TIEMI TANIGUTI, LETÍCIA MONTANHOLI APOLINÁRIO, HUMBERTO SANTO NETO, MARIA JULIA MARQUES A - 48 MIDGUT OF THE DIPLOPOD UROSTREPTUS ATROBRUNNEUS: STRUCTURE, FUNCTION AND REDEFINITION OF HEPATIC CELLS. CRISTINA MOREIRA DE SOUSA, ALDINEI GONÇALVES JUNIOR, CARMEM SILVIA FONTANETTI, MONIKA IAMONTE A - 49 DIFFERENTIAL EFFECTS OF CHEMOATTRACTANTS ON MAST CELL RECRUITMENT. MARIA RITA DE CÁSSIA CAMPOS, CONSTANCE OLIVER, MARIA CELIA JAMUR A - 50 RESERVE AND URATE STORAGE IN THE FAT BODY CELLS DURING SOLDIER DIFFERENTIATION IN THE TERMITE HETEROTERMES TENUIS (ISOPTERA, RHINOTERMITIDAE). LARA TEIXEIRA LARANJO, ANA MARIA COSTA LEONARDO A - 56 CYTOCHEMISTRY OF CLUB CELLS IN THE EPIDERMIS OF THE CATFISH PIMELODELLA CF. LATERISTRIGA LICHTENSTEIN, 1823 (OSTARIOPHYSI: SILURIFORMES). LUIZ FERNANDO DUBOC, JULIANA CASTRO MONTEIRO, KARINA CARVALHO MANCINI, EDUARDO MEDEIROS DAMASCENO A - 57 ULTRASTRUCTURE OF CLUB CELLS IN THE EPIDERMIS OF THE NEOTROPICAL CATFISH PIMELODELLA CF. LATERISTRIGA LICHTENSTEIN, 1823 (OSTARIOPHYSI: SILURIFORMES. EDUARDO MEDEIROS DAMASCENO, LUIZ FERNANDO DUBOC, JULIANA CASTRO MONTEIRO, KARINA CARVALHO MANCINI A - 58 TREATMENT WITH VOCHYSIA SP (VOCHYSIACEAE) EXTRACT IN STREPTOZOTOCIN-INDUCED DIABETIC RATS ATTENUATED GLYCEMIA WITHOUT ALTERING MORPHOLOGY OF HEPATIC TISSUE. IZABELA BARBOSA MORAES, CAMILLA MANZAN MARTINS, NEIRE MOURA DE GOUVEIA, LUCIANA KAREN CALÁBRIA, KAREN RENATA NAKAMURA HIRAKI, ALBERTO DA SILVA MORAES, FOUED SALMEN ESPINDOLA A - 59 STRATEGIES FOR IMMOBILIZATION OF MESENCHYMAL STEM CELLS USING SUPER-HYDROPHILIC VERTICALLY ALIGNED CARBON NANOTUBES AND DISPERSED MAGNETICALLY ORIENTED CARBON NANOTUBES. ALESSANDRO EUSTAQUIO CAMPOS GRANATO, LAYLA TESTA GALINDO, TAÍS ADELITA BARROS, MARCELLA BRAGA DA COSTA REIS, PIERO BAGNARESI, LILIAN SIQUEIRA, ANDERSON DE OLIVEIRA LOBO, MARIMÉLIA PORCIONATTO A - 60 MEDIUM-TERM TREATMENT WITH CYCLOSPORIN A AND HETEROPTERYS TOMENTOSA, INVESTIGATED IN HEPATIC TISSUE OF WISTAR RATS. KARINE DE MOURA FREITAS, MARIA APARECIDA DA SILVA DIAMANTE, JACQUELINE MERIELLEN DE ALMEIDA, NAYARA RUDECK COCK, MARÇAL HENRIQUE AMICI JORGE, MARY ANNE HEIDI DOLDER A - 61 CLONING, EXPRESSION AND BIOLOGICAL CHARACTERIZATION OF A NOVEL PHOSPHOLIPASE-D FROM BROWN SPIDER (LOXOSCELES INTERMEDIA) VENOM. GABRIEL OTTO MEISSNER, LARISSA VUITIKA, DILZA TREVIZAN SILVA, LUIZA HELENA GRESMKI, OLGA MEIRI CHAIM, MATHEUS REGIS BELISÁRIO, ADRIANO MARCELO MORGON, SILVIO SANCHES VEIGA A - 62 EFFECT OF ACARBOSE AND PHASEOLAMINE TREATMENTS ABOUT LIVER MORPHOLOGY IN STREPTOZOTOCIN-INDUCED DIABETIC RATS. CAMILLA MANZAN MARTINS, IZABELA BARBOSA MORAES, KAREN RENATA NAKAMURA HIRAKI, NEIRE MOURA GOUVEIA, LUCIANA KAREN CALÁBRIA, FOUED SALMEN ESPINDOLA A - 63 MELATONIN EFFECTS IN PROSTATE HISTOPHYSIOLOGY OF PREPUBERTAL RATS SUBJECTED TO SHORT TERM DIABETES. MARINA GUIMARÃES GOBBO, GUILHERME HENRIQUE TAMARINDO, VIVIANE SANCHES MASITÉLI, CAROLINA FRANDSEN PEREIRA COSTA, SEBASTIÃO ROBERTO TABOGA, REJANE MAIRA GÓES A - 64 TOXICITY IN LIVER OF FISH SPECIES OREOCHROMIS NILOTICUS (CICHLIDAE) EXPOSED TO NICL2. AMANDA ALFONSO BATISTA, CINTYA A CHRISTOFOLETTI, CARMEM S. FONTANETTI A - 65 EFFECT OF VOCHYSIA SP. EXTRACT ON THE MORPHOMETRY OF THE PAROTID GLAND IN STREPTOZOTOCIN-INDUCED DIABETIC RATS. DOUGLAS CARVALHO CAIXETA, FRANCYELLE BORGES ROSA DE MOURA, ALICE VIEIRA DA COSTA, LUCIANA KAREN CALÁBRIA, MARCELO EMÍLIO BELETTI, FOUED SALMEN ESPINDOLA A - 66 IN SILICO ANALYSIS OF BINDING PEPTIDES TO CELL SURFACE FROM STAPHYLOCOCCUS AUREUS. FERNANDO VIEIRA RODRIGUES, EMÍLIA REZENDE VAZ, CAROLINE FERNANDES REIS, LÉA DUARTE DA SILVA MORAES, YARA CRISTINA DE PAIVA MAIA, CARLOS UEIRA VIEIRA, LUIZ RICARDO GOULART, TATIANA AMABILE DE CAMPOS A - 67 HISTOLOGY AND HISTOCHEMICAL CHARACTERIZATION OF THE STOMACH STRUCTURE OF ANTARCTIC FISH NOTOTHENIA ROSSII (RICHARDSON, 1844) UNDER CONDITIONS OF THERMAL STRESS. PRISCILA KREBSBACH, AXEL H. R. COFRÉ, CINTIA MACHADO, MARIA ROSA D. PEDREIRO, FLÁVIA B. V. SILVA, 88 TÂNIA ZALESKI, LUCIANA B. CETTINA, MARIANA FORGATI, CLÁUDIO A. PIECHNIK, LUCÉLIA DONATTI A - 68 ASSESSMENT OF SUBCHRONIC ORAL TOXICITY OF ETHANOLIC EXTRACT OF MAYTENUS ILICIFOLIA MART. EX REISSEK. IN MALE AND FEMALE WISTAR RATS. SILVANE SOUZA ROMAN, CARLA GIANE LOSS, TAÍS REGINA FIORENTIN, FABIOLA REGINA BREDA, GABRIELA GUBERT, MORGANA PISTORE, JANAINA VIEIRA BELUSSO, MICHELA BIANCHI DE MELLO, ARNO ERNESTO HOFMANN JUNIOR A - 69 INVESTIGATION OF NTPDASE-2 ROLE IN CELL ADHESION, PROLIFERATION AND MIGRATION. FRANCIELE CRISTINA KIPPER, DARLAN CONTERNO MINUSSI, GUIDO LENZ, MÁRCIA ROSÂNGELA WINK A - 70 THE CHARACTER PROTEIN OF PROSTATE GERBIL: MALE INTACT AND FEMALE RECEIVED SUPPLEMENTATION OF TESTOSTERONE. PEDRO HENRIQUE GARCIA SOBRINHO, ANA PAULA DA SILVA PEREZ, FÁTIMA PEREIRA DE SOUZA, SEBASTIÃO ROBERTO TABOGA A - 71 ENDOPLASMIC RETICULUM STRESS IS AN EVOLVING FEATURE OF AORTIC DISEASE IN HETEROZYGOUS MARFAN SYNDROME MICE, WHILE NOT ACCOUNTED FOR BY FIBRILLIN-1 MUTATION ITSELF. THAYNA MEIRELLES SANTOS, MARIA CAROLINA GUIDO, VICTOR DEBBAS, LYGIA DA VEIGA PEREIRA, FRANCISCO RAFAEL MARTINS LAURINDO A - 72 BIOCOMPATIBILITY EVALUATION OF SOLID LIPID NANOPARTICLES TOWARD MOUSE EMBRYO FIBROBLASTS AND ERITHROCYTES. ADNY HENRIQUE SILVA, CARINE DAL PIZZOL, FABÍOLA B. FILIPPIN-MONTEIRO, ÂNGELA M. DE CAMPOS, TÂNIA B.CRECZYNSKI-PASA A - 73 ARREST OF CYTOPLASMIC STREAMING INDUCES ALGAL PROLIFERATION IN GREEN PARAMECIA. HIROSHI HOSOYA, EIJI HIRAKI, KOYO TETSUKAWA, YOSHIHIKO YAMASHIN, TOSHIYUKI TAKAHASHI, KOZUE HAMAO A - 74 NITRIC OXIDE PRODUCTION BY HEMOCYTES OF THE ASCIDIAN PHALLUSIA NIGRA. LAURA CARRIELLO EMRICH, DANIELLY DA FONTE CARVALHO MARTINS, RODRIGO NUNES DA FONSECA, SILVANA ALLODI, CINTIA MONTEIRO DE BARROS A - 75 DIPHOSPHORYLATED MYOSIN II REGULATORY LIGHT CHAIN LOCALIZES TO THE MIDZONE WITHOUT ITS HEAVY CHAIN DURING CYTOKINESIS. TOMO KONDO, KEIJU KAMIJO, KOZUE HAMAO, HIROSHI HOSOYA A - 85 MAINTENANCE OF BIOCHEMICAL PROPERTIES OF CHONDROCYTES IN VITRO UNDERGOING CHONDROGENIC MEDIUM RICH IN FACTOR. RICARDO SCHMID BOMFIM, CAMILA BASILE CARBALLO A - 86 RECOMBINANT PHOSPHOLIPASE-D FROM BROWN SPIDER VENOM (LOXOSCELES GENUS) INDUCES CYTOSOLIC CALCIUM INFLUX AND DEGRADATION OF PLASMA MEMBRANE PHOSPHOLIPIDS OF B16-F10 CELLS. ANA CAROLINA MARTINS WILLE, DANIELLE CHAVES MOREIRA, MARIANA G. MAGNONI, THIAGO LOPES DE MARI, LUIZA HELENA GREMSKI, OLGA MEIRI CHAIM, ANDREA SENFF RIBEIRO, SILVIO SANCHES VEIGA A - 87 DOUBLE STRAND BREAK REPAIR PROTEINS IDENTIFICATION IN MAMMALIAN MITOCHONDRIA. VALQUIRIA TIAGO DOS SANTOS, NADJA CRISTHINA DE SOUZA PINTO A - 88 THE PRESENCE OF THE SYMBIOTIC BACTERIUM INFLUENCES THE O2 CONSUMPTION IN ITS HOST CELL, ANGOMONAS DEANEI. ALLAN CÉZAR DE AZEVEDO MARTINS, ANA CAROLINA LOYOLA MACHADO, ANTÔNIO GALINA, LUCIANE CIAPINA, LUIZ GONZAGA, ANA TEREZA RIBEIRO VASCONCELOS, WANDERLEY DE SOUZA, MARCELO EINICKER LAMAS, MARIA CRISTINA MACHADO MOTTA A - 89 THE TETRASPANIN CD63 IS HIGHLY EXPRESSED BY SECRETORY GRANULES IN HUMAN BLOOD EOSINOPHILS. LÍVIA ANDRESSA SILVA DO CARMO, KÁTIA BATISTA DO AMARAL, ANN M. DVORAK, PETER F. WELLER, ROSSANA CORREA NETTO DE MELO A - 90 THE INFLUENCE OF THE SYMBIOTIC BACTERIUM ON RESPIRATION OF HOST TRYPANOSOMATIDS STRIGOMONAS CULICIS. ANA CAROLINA LOYOLA MACHADO, DALLAN CÉZAR DE AZEVEDO MARTINS, ANTÔNIO GALINA FILHO, WANDERLEY DE SOUZA, MARIA CRISTINA MACHADO MOTTA A - 91 AGE–RELATED CHANGES IN LIVER MORPHOLOGY: COULD HETEROPTERYS TOMENTOSA ALLEVIATE THESE CHANGES? MARIA APARECIDA DA SILVA DIAMANTE, FABRICIA DE SOUZA PREDES, ANA MILENA HERRERA, JULIANA DE CASTRO MONTEIRO, MARY ANNE HEIDI DOLDER A - 92 HISTOPATHOLOGICAL CHANGES IN THE ANTARCTIC FISH NOTOTHENIA CORIICEPS AND NOTOTHENIA ROSSII COLLECTED IN KING GEORGE ISLAND, ANTARCTIC PENINSULA. MARIA ROSA DMENGEON PEDREIRO, FLÁVIA SANT`ANNA RIOS, CINTIA MACHADO, PRISCILA KREBSBACH, CLÁUDIO ADRIANO PIECHNIK, TANIA ZALESKI, MARIANA FORGATI, LUCIANA BADELUK CETTINA, EDSON RODRIGUES, LUCÉLIA DONATTI A - 76 COMPARATIVE HISTOLOGY OF THE SPLEEN OF THREE TELEOST SPECIES CAPTURED IN THE ITAPECERICA RIVER, DIVINÓPOLIS, MG, BRAZIL. MARILIA GABRIELA C AMARAL, REGIANNE FERREIRA SILVA, CAMILA FERREIRA SALES, HEDER J. RIBEIRO, ROSY I. MACIEL AZAMBUJA RIBEIRO, FABRICIO FLÁVIO THEOPHILO DOMINGOS, RALPH GRUPPI THOMÉ, HÉLIO BATISTA DOS SANTOS A - 93 TC95 A HYBRID MOLECULE ON LEISHMANIA AMAZONENSIS: NEW CELLULAR TARGETS. JOSEANE LIMA PRADO GODINHO, K. GEORGIKOPOULOU, T. CALOGEROPOULOU, WANDERLEY DE SOUZA, JULIANY COLA FERNANDES RODRIGUES A - 77 USING A CACO-2 CELL CULTURE MODEL FOR DRUG PERMEATION. JULIANNA HENRIQUES DA SILVA, VIVIANE LIONE, RITA DE CASSIA ASCENÇÃO BARROS, CARLOS RANGEL RODRIGUES, HELENA CARLA CASTRO, VALERIA P. SOUSA, LUCIO MENDES CABRAL, LUIZ EURICO NASCIUTTI A - 94 BIOLOGY OF CIRCULATING ENDOTHELIAL PROGENITOR CELLS IN THE CONTEXT OF OBESITY IN ADOLESCENTS. BRUNO DIAZ PAREDES, ELIETE BOUSKELA, LUIZ GUILHERME KRAEMER DE AGUIAR, VERÔNICA MORANDI, MARCELA FERREIRA MARQUES A - 78 NOREPINEPHRINE DEPRESSES THE NITRIC OXIDE PRODUCTION IN THE ASCIDIAN PHALLUSIA NIGRA HEMOCYTES. ANDRESSA DE ABREU MELLO, SILVANA ALLODI, CINTIA MONTEIRO DE BARROS A - 95 ULTRASTRUCTURAL AND CYTOGENETIC CHARACTERIZATION OF SOME ASPECTS OF SPERMATOGENESIS OF ZAPRIONUS INDIANUS AND ZAPRIONUS SEPSOIDES. LETÍCIA DO NASCIMENTO ANDRADE DE ALMEIDA REGO, ROSANA SILISTINO DE SOUZA, MARIA TERCÍLIA VILELA DE AZEREDO OLIVEIRA, LILIAN MADI-RAVAZZI A - 79 PURINERGIC RECEPTORS ARE INVOLVED IN THE ACTIVATION OF MACROPHAGES BY URIC ACID CRYSTALS THROUGH THE NLRP3-INFLAMMASOME PATHWAY. THOMAS GICQUEL, TATIANA VICTONI, ALAIN FAUTREL, FLORENCE GLEONNEC, CARINE LAMBERT, CARINE LAMBERT, ISABELLE COUILLIN, ELISABETH BOICHOT, VINCENT LAGENTE A - 80 ADDITIVE EFFECT OF CIGARETTE SMOKE EXTRACT (CSE) AND LIPOLYSACCHARIDE (LPS) ON PROINFLAMMATORY CYTOKINE RELEASE THROUGH ACTIVATION OF JAK/STAT PATHWAYS IN HUMAN AIRWAY EPITHELIAL CELLS. TATIANA VICTONI, MANUELLA LANZETTI, FLORENCE GLEONNEC, LUCIE BEAUTRAIS, SAMUEL S. VALENÇA, LUIS CRISTOVÃO PORTO, ELISABETH BOICHOT, VINCENT LAGENTE A - 81 EFFECTS OF TREATMENTS WITH DIFFERENT ULTRASOUND FIELDS IN SKELETAL MUSCLE CELL CULTURES. VIVIANE M. ABRUNHOSA, CAROLINA PONTES SOARES, RODRIGO COSTA-FELIX, MANOEL COSTA, CLAUDIA MERMELSTEIN A - 82 EFFECTS OF ANTIFUNGAL ON SPOROTHRIX SCHENCKII. LUANA PEREIRA BORBA DOS SANTOS, KELLY ISHIDA, LEILA MARIA LOPES BEZERRA, SONIA ROZENTAL A - 83 LIPID DROPLETS INDUCTION IN MURINE MACROPHAGES WITH MICE SERUM: POSSIBLE PROTEINACEOUS NATURE OF THE INDUCTION FACTOR. THIAGO TORRES DE AGUIAR, LAURA AZEREDO MIRANDA MOTA, SÉRGIO HENRIQUE SEABRA, PATRÍCIA TORRES BOZZA, GEÓRGIA CORREA ATELLA, RENATO AUGUSTO DAMATTA A - 84 HIGH-FAT DIET ENRICHED WITH FISH OIL DECREASES LYMPHOCYTE ACTIVATION IN MICE. HELOÍSA HELENA DE OLIVEIRA ALVES, CESAR MIGUEL MOMESSO, JARLEI FIAMONCINI, KIM GUIMARÃES CAÇULA, MARIA FERNANDA CURY-BOAVENTURA, SANDRO MASSAO HIRABARA, RUI CURI, RENATA GORJÃO A - 96 EVIDENCES OF DEGRANULATION IN HOLOTHURIA SPHERULOCYTES. PATRICIA LACOUTH, MÁRCIO REIS CUSTÓDIO GRISEA A - 97 CELLULAR RESPONSE OF SKIN FIBROBLAST TO LEISHMANIA (LEISHMANIA) AMAZONENSIS INFECTION. CAMILA GUERRA SILVA, ROGER MAGNO MACEDO SILVA, CARINA DE LIMA PEREIRA DOS SANTOS, VANESSA ALVARO DINIZ, SUZANA CÔRTE-REAL A - 98 ADENOHYPOPHYSEAL CELLS IN ADULTS OF SALMINUS BRASILIENSIS (TELESOSTEI, CHARACIFORMES): A HISTOCHEMYCAL AND IMUNOHISTOCHEMYCAL STUDY. LÁZARO WENDER OLIVEIRA DE JESUS, CHAYRRA CHERRADE GOMES, GISELE CRISTIANE DE MELO DIAS, SARA ZAGO GOMES, CRUZ ALBERTO MENDOZA RIGONATI, MARIA INÊS BORELLA A - 99 EVALUATION OF BME26 CELLS RESISTANCE TO HYDROGEN PEROXIDE INDUCED STRESS. BÁRBARA PITTA DELLA NOCE, JORGE LUIS RIBEIRO, RENATO AUGUSTO DAMATTA, CARLOS LOGULLO A - 100 INSIGHTS ABOUT FIBROCYTES PARTICIPATION IN THE IMMUNE RESPONSE OF LEISHMANIASIS. CARINA DE LIMA PEREIRA DOS SANTOS, THAISA VIEIRA, VANESSA ALVARO DINIZ, CAMILA GUERRA SILVA, ROGER MAGNO MACEDO SILVA, JORGE JOSÉ DE CARVALHO, SUZANA CÔRTE-REAL A - 101 THE ROLE OF HISTONE H4 IN THE TRYPANOSOMA CRUZI CHROMATIN. THIAGO CESAR PRATA RAMOS, BRUNO DOS SANTOS PASCOALINO, SHEILA CRISTINA NARDELLI, SERGIO SCHENKMAN A - 102 CELLULAR REDISTRIBUTION OF A PROTEIN KINASE INVOLVED IN TRANSLATION CONTROL DURING DIFFERENTIATION OF TRYPANOSOMA CRUZI. LEONARDO DA SILVA AUGUSTO, NILMAR SILVIO MORETTI, SERGIO SCHENKMAN A - 103 ICK PEPTIDE FROM LOXOSCELES INTERMEDIA VENOMOUS GLAND: CLONING, EXPRESSION AND PRODUCTION OF POLYCLONAL ANTIBODIES. 89 FERNANDO HITOMI MATSUBARA, EDUARDO SOARES CONSTANTINO LOPES, GABRIEL OTTO MEISSNER, VALÉRIA PEREIRA FERRER, LUIZA HELENA GREMSKI, ANDREA SENFF RIBEIRO, OLGA MEIRI CHAIM, SILVIO SANCHES VEIGA A - 104 STUDY OF THE VENTRAL PROSTATE EMBRYOGENESIS IN MONGOLIAN GERBIL. BRUNO DOMINGOS AZEVEDO SANCHES, MANOEL FRANCISCO BIANCARDI, LUIZ ROBERTO FALLEIROS-JR, FERNANDA CRISTINA ALCANTARA DOS SANTOS, SEBASTIÃO ROBERTO TABOGA A - 105 TGFB EFFECT IN NUCLEAR TRANSLOCATION OF CLUSTERIN IN RWPE-1 TRANSFECTED WITH A CLU-GFP CLONE AND ITS IMMUNOLOCALIZATION IN DU145 AND PC3 CELLS. FABIANA KUHNE, GUSTAVO DE MAGALHÃES ALMEIDA, HERNANDES F CARVALHO A - 106 THYROID HORMONE (T3) MODULATES THE ENTERIC GLIA. ANA CARINA BON FRAUCHES OLIVEIRA, SUZANA ASSAD KAHN, ANA LÚCIA TAVARES GOMES, PATRÍCIA CASTELUCCI, VIVALDO MOURA NETO A - 107 RODLET CELLS IN GILL EPITHELIUM OF CURIMBA (PROCHILODUS ARGENTEUS): ULTRASTRUCTURE AND S100 IMMUNOREACTIVITY. MARCELA SANTOS PROCÓPIO, HEDER JOSÉ RIBEIRO, SAMYRA MARIA DOS SANTOS NASSIF LACERDA, PATRÍCIA MASSARA MARTINELLI, JOSÉ DIAS CORRÊA JUNIOR A - 108 HISTOCHEMICAL AND BIOCHEMICAL IDENTIFICATION OF THE GLYCOCONJUGATE TYPE IN SECRETION OF THE PAROTOID GLAND OF A BRAZILIAN TOAD (RHINELLA ICTERICA). JULIANE SIQUEIRA FRANCISCO, ELIENE OLIVEIRA KOZLOWSKI DE FARIAS, MAURO SÉRGIO GOLÇALVES PAVÃO, LYCIA DE BRITO GITIRANA A - 109 5-HYDROXY-2-HYDROXYMETHYL-PYRONE (HMP) AS MACROPHAGE ACTIVATOR. ANA PAULA DRUMMOND RODRIGUES, LUIS HENRIQUE SEABRA DE FARIAS, ALBERDAN SILVA SANTOS, JOSÉ LUIZ MARTINS DO NASCIMENTO, EDILENE OLIVEIRA DA SILVA A - 110 EVALUATION OF LEISHMANICIDAL ACTIVITY OF RHIZOPHORA MANGLE ON LEISHMANIA MAJOR. KRISIA EMANUELLE FERREIRA DA SILVA, ERWELLY BARROS DE OLIVEIRA, MARLLON ALEX NASCIMENTO SANTANA, PAULO HENRIQUE CAVALCANTI DE ARAÚJO, ANTÔNIO FERNANDO MORAIS DE OLIVEIRA, PALOMA LYS DE MEDEIROS, ELIETE CAVALCANTI DA SILVA, JEYMESSON RAPHAEL CARDOSO VIEIRA A - 111 EFFECTS OF 5-HYDROXY-2-HYDROXYMETHYL-GAMMA-PYRONE (HMP), A SECONDARY METABOLITE OBTAINED FROM ASPERGILLUS FUNGI, ON HUMAN NEUTROPHILS. PAULA CRISTINA RODRIGUES FRADE, JOSINEIDE PANTOJA DA COSTA, ANA PAULA DRUMMOND RODRIGUES, LUIS HENRIQUE SEABRA FARIAS, BRUNO JOSÉ MARTINS DA SILVA, RAQUEL RAICK PEREIRA DA SILVA, AMANDA ANASTÁCIA PINTO HAGE, CAROLINE MARTINS ALMEIDA, ALBERDAN SILVA SANTOS, EDILENE OLIVEIRA DA SILVA A - 112 RST-NEPH PROTEIN FAMILY IN CHICK EMBRYOS. MARA SILVIA ALEXANDRE COSTA, FELIPE MONTELEONE VIECELI, LUANA CRISTINA AMISTA, JOSÉ EDUARDO BARONEZA, MAIARO CABRAL ROSA MACHADO, IRENE YAN, RICARDO GUELERMAN PINHEIRO RAMOS A - 113 EFFECTS OF 5-HYDROXY-2-HYDROXYMETHYL--PYRONE (HMP) IN FILAMENTOUS FUNGAL CURVULARIA PALLESCENS. JORGE AUGUSTO LEÃO PEREIRA, ANA PAULA DRUMMOND RODRIGUES, DAVI MARCOS DE SOUZA OLIVEIRA, ALBERDAN SILVA SANTOS, EDILENE OLIVEIRA DA SILVA A - 114 DIFFERENTIATION OF HUMAN MONOCYTES IN VITRO BY 5HYDROXY-2-HYDROXYMETHYL-GAMMA-PYRONE (HMP), A BIOPRODUCT OBTAINED FROM ASPERGILLUS FUNGI. JOSINEIDE PANTOJA DA COSTA, PAULA CRISTINA RODRIGUES FRADE, ANA PAULA DRUMMOND RODRIGUES, BRUNO JOSÉ MARTINS DA SILVA, ALBERDAN SILVA SANTOS, EDILENE OLIVEIRA DA SILVA A - 115 DELETERIOUS EFFECTS OF WATER-SOLUBLE FRACTION (WSF) OF PETROLEUM ON BEIJUPIRÁ (RACHYCENTRON CANADUM): HISTOLOGICAL EVALUATION. KARINA FERNANDES REZENDE, LÍGIA MARIA SALVO, JULIANA CRISTINA TEIXEIRA DE MORAES, DIVINOMAR SEVERINO, JOSÉ ROBERTO MACHADO CUNHA DA SILVA A - 116 ANALYSIS OF LIPID DROPLETS IN LEISHMANIA (VIANNIA) BRAZILIENSIS PROMASTIGOTES IN THE EARLY STATIONARY PHASE OF THE GROWTH. AMANDA ANASTÁCIA PINTO HAGE, NUCCIA N. T. DE CICCO, GEORGIA C. ATELLA, RAQUEL RAICK PEREIRA DA SILVA, BRUNO JOSÉ MARTINS SILVA, PAULA CRISTINA R. FRADE, ANA PAULA DRUMMOND RODRIGUES, EDILENE OLIVEIRA DA SILVA A - 117 Α6Β1 INTEGRIN IS RELEVANT IN THE FORMATION AND SURVIVAL OF DIFFERENTIATED 3D ACINI OF SALIVARY GLANDS CELLS: IMPLICATIONS IN SJÖGREN’S SYNDROME. HERY ANDRÉS URRA ZÚÑIGA, DENISSE SEPULVEDA, JUAN CORTES, VERONICA BAHAMONDES, ISABEL CASTRO, MARIA JOSE BARRERA, SERGIO AGUILERA, CLAUDIO MOLINA, CECILIA LEYTON, CECILIA ALLENDE, SERGIO GONZALEZ, MARÍA JULIETA GONZÁLEZ A - 118 FURTHER STUDIES OF THE IN SITU CELL STRUCTURE OF CELLS AND TISSUES WITH THE ATOMIC FORCE MICROSCOPE. MARÍA DE LOURDES SEGURAVALDEZ, GEORGINA ALVAREZ-FERNÁNDEZ, ALMA ZAMORA-CURA, ASIER GARCÍA SENOSIAIN, LOURDES TERESA AGREDANO-MORENO, LUIS FELIPE JIMÉNEZ-GARCÍA A - 119 DISTRIBUTION OF AMINOPEPTIDASES IN SUBCELLULAR FRACTIONS OF ADIPOCYTES FROM ABDOMINAL FAT IN MONOSODIUM GLUTAMATE OBESITY. RAFAELA FADONI ALPONTI, PAULO FLAVIO SILVEIRA A - 120 MELANOPSIN ACTIVATES CLOCK GENES IN ZEBRAFISH CELLS. BRUNO CESAR RIBEIRO RAMOS, MARIA NATHÁLIA DE CARVALHO MAGALHÃES MORAES, LEONARDO HENRIQUE RIBEIRO GRACIANI DE LIMA, ANA MARIA DE LAURO CASTRUCCI A - 121 HISTOPATHOLOGICAL CHANGES IN THE GILLS OF ARAPAIMA GIGAS AFTER BATHS IN FORMALDEHYDE SOLUTION. JOSÉ CARLOS NUNES RAULINO, SANNY MARIA DE ANDRADE-PORTO, LUCIANA ARRUDA DINÓLA DE OLIVEIRA, JOSE CELSO DE OLIVEIRA MALTA A - 122 MUCOUS CELLS IN GILLS OF ARAPAIMA GIGAS EXPOSED TO WHITE AND BLACK WATERS AND ITS INFLUENCE ON FISH HOMEOSTASIS. JOSÉ CARLOS NUNES RAULINO, JANILSON MORAES SERUDO, ELIZABETH GUSMÃO AFFONSO, OSCAR TADEU FERREIRA DA COSTA, WALLICE LUIZ PAXIÚBA DUNCAN, MARISA NARCISO FERNANDES, CLEVERSON AGNER RAMOS B – Cell Biology and Cancer B1-B252 B-1 NUCLEAR CALCIUM BUFFERING SENSITIZES HUMAN SQUAMOUS CELL CARCINOMA TO X-RAYS USING HEAD AND NECK RADIOTHERAPY PROTOCOL. LÍDIA MARIA DE ANDRADE, JONY MARQUES GERALDO, OSVALDO XAVIER GONÇALVES, MIGUEL TORRES TEIXEIRA LEITE, ANDERSON MIRANDA CATARINA, ADRIANA FRANCO PAES LEME, SAMI YOOKO, CARLOS RENATO MACHADO, MATHEUS ANDRADE RAJÃO, RODRIGO RIBEIRO RESENDE, CARLA JEANE AGUIAR, ELAINE MARIA DE SOUZA FAGUNDES, CARLOS LEOMAR ZANI, OLINDO ASSIS MARTINS FILHO, MARIA DE FÁTIMA LEITE B-2 EVALUATION OF GENOTOXICITY OF SOLANUM LYCOCARPUM AQUEOUS EXTRACTS UTILIZING ALLIUM CEPA TEST-SYSTEM. VIVIANE MOREIRA DE LIMA, JÉSSICA TAMARA DOS SANTOS, JENNIFER VIEIRA GOMES, MARIANA DA SILVA DE MELLO, PATRÍCIA FAMPA, HÉLCIO RESENDE BORBA B-3 ASSOCIATION BNP LEVEL WITH CARDIAC DAMAGES INDUCED BY IONIZING RADIATION AND CHEMOTHERAPY DRUGS. VERA MARIA ARAÚJO DE CAMPOS, CAMILA SALATA, CHERLEY BORBA VIEIRA DE ANDRADE, SAMARA CRISTINA FERREIRA MACHADO, ADENILSON DE SOUZA DA FONSECA, ANA LUCIA ROSA NASCIMENTO, JORGE JOSÉ DE CARVALHO, CARLOS EDUARDO VELOSO DE ALMEIDA B-4 TRICHOSTATIN A, A HISTONE DEACETYLASE INHIBITOR, AFFECTS GLIOMA TUMORSPHERES FORMATION AND GROWTH. FELIPE DE ALMEIDA SASSI, ANA LUCIA ABUJAMRA, RAFAEL ROESLER B-5 SUPPRESSION OF CLAUDIN-7 EXPRESSION PROMOTES CELL PROLIFERATION AND DISRUPTS CELL-MATRIX INTERACTIONS IN HUMAN LUNG CANCER CELLS. ZHE LU, QUN LU, LEI DING, YAN-HUA CHEN B-6 MODULATION OF CANONICAL WNT SIGNALING PATHWAY DURING EXPERIMENTAL ORAL CARCINOGENESIS. JULIANA GONÇALVES CARVALHO, JULIANA NOGUTI, CAROLINA PRADO DE FRANÇA CARVALHO, MARCELLO FRANCO, CELINA TIZUKO FUJIYAMA OSHIMA, DANIEL ARAKI RIBEIRO B-7 SCREENING OF PLANTS EXTRACTS OF CERRADO IN NOT CLINICAL ASSAYS FOR ANTITUMOR ACTIVITY. CAMILA RAQUEL RODRIGUES BARBOSA, HELOÍSA HELENA MARQUES OLIVEIRA, LUCIANA MARIA SILVA, VERA LÚCIA DE ALMEIDA, CAROLINA PAULA DE SOUZA MOREIRA B-8 PRELIMINARY STUDIES OF PROSTATE TUMOR CELLS INTERACTIONS WITH BRAIN MICROENVIRONMENT. ELIANE GOUVEA DE OLIVEIRA, ANTONIO PALUMBO JUNIOR, CELIA YELIMAR PALMERO, LEANDRO MIRANDA-ALVES, CHRISTINA MAEDA TAKIYA, VIVALDO MOURA-NETO, LUIZ EURICO NASCIUTTI B-9 RAF KINASE INHIBITOR (RKIP) DEPLETION IS ASSOCIATED WITH CERVICAL CANCER AGGRESSIVENESS. OLGA CATARINA LOPES MARTINHO, FILIPE PINTO, SARA GRANJA, VERA MIRANDA-GONÇALVES, MARISE A.R. MOREIRA, LUIS F.J. RIBEIRO, CELSO DI LORETO, MARSHA R. ROSNER, ADHEMAR LONGATTO-FILHO, RUI MANUEL REIS B - 10 IDENTIFICATION OF NOVEL POTENTIAL PREDICTIVE TARGETS TO ANTI-ANGIOGENIC THERAPY RESPONSE IN GLIOBLASTOMAS. OLGA CATARINA LOPES MARTINHO, VERA MIRANDA-GONÇALVES, RUI MANUEL REIS B - 11 IMMUNOHISTOCHEMICAL EXPRESSION OF BETA-CATENIN, WNT-1 AND C-MYC IN BASAL CELL ADENOMAS FROM SALIVARY GLAND. JOÃO PAULO SILVA SERVATO, ADRIANO MOTA LOYOLA, ANA LÚCIA AMARAL EISENBERG, FERNANDO LUIZ DIAS, PAULO ROGÉRIO DE FARIA, SÉRGIO VITORINO CARDOSO B - 12 CITOTOXICITY BY SELENIUM IN LUNG ADENOCARCINOMA CELLS. LUDMILLA REGINA DE SOUZA DAVID, MAYARA SIMONELLY COSTA DOS SANTOS, MARÍLIA CRISTINA ROSA DA COSTA, LUIS ALEXANDRE MUEHLMANN, RICARDO BENTES DE AZEVEDO, SÔNIA NAIR BÁO 90 B - 13 ANTITUMORAL POTENTIAL ABILITY OF EXTRACELLULAR ATP AGAINST LEUKEMIC STEM CELLS. ANTONIO CARLOS RIBEIRO FILHO, EDGAR JULIAN PAREDES GAMERO, CHRISTIANO MARCELO VAZ BARBOSA, AMANDA NOGUEIRA PEDRO B - 14 CARDIAC ALTERATIONS INDUCED BY DOCETAXEL AND CYCLOPHOSPHAMIDE. VERA MARIA ARAÚJO DE CAMPOS, SAMARA CRISTINA FERREIRA-MACHADO, CAMILA SALATA, CAMILA SALATA, NAZARETH NOVAES ROCHA, CARLOS ALBERTO MANDARIM-DE-LACERDA, CARLOS EDUARDO DEALMEIDA B -15 CELL DEATH INDUCED BY RHODIUM (II) CITRATE LOADED MAGNETIC NANOPARTICLES IN BREAST CANCER CELLS. NATALIA LEMOS CHAVES, JOSÉ RAIMUNDO CORRÊA, MARCELLA LEMOS BRETAS, APARECIDO RIBEIRO DE SOUZA, SÔNIA NAIR BÁO B -16 LOW-INTENSITY INFRARED LASER EXPOSURE ALTERS EXPRESSION OF DNA REPAIR GENES. ADENILSON DE SOUZA DA FONSECA, VERA MARIA ARAÚJO DE CAMPOS, SAMARA CRISTINA FERREIRA-MACHADO, ANTÔNIO AUGUSTO DE FREITAS PEREGRINO, CARLOS EDUARDO VELOSO DE ALMEIDA, ANDRÉ LUIZ MENCALHA, MAURO GELLER, FLÁVIA DE PAOLI B -17 CASPASE-3 ACTIVATION AND INCREASED PROCOLLAGEN TYPE I IN RADIATION HEART INDUCED LATE EFFECTS. SAMARA CRISTINA FERREIRAMACHADO, CAMILA SALATA, NAZARETH DE NOVAES ROCHA, ALEXANDRE FELIPE SILVA CORRÊA, SUZANA CÔRTE-REAL FARIA, VERA MARIA ARAÚJO DE CAMPOS, CHERLEY BORBA VIEIRA DE ANDRADE, ANTÔNIO AUGUSTO DE FREITAS PEREGRINO, ADENILSON DE SOUZA DA FONSECA, FLÁVIA DE PAOLI, CARLOS EDUARDO DEALMEIDA B - 18 ACTIVATED WNT SIGNALING PATHWAY IS NOT INFLUENCED BY ABSENCE OF GALECTIN-3 IN MICE DURING TONGUE MALIGNANT TRANSFORMATION. MARCUS VINÍCIUS RODRIGUES DE SOUZA, MONICA LUIZA CAMARGOS LOPES, WANDERSON DE ALMEIDA RAMOS, ROGER CHAMMAS, JULIANA MOREIRA DE ALMEIDA SANT’ANA, DANIELLA FERNANDES MENDONÇA, ADRIANO MOTA LOYOLA, SÉRGIO VITORINO CARDOSO, PAULO ROGÉRIO DE FARIA B - 19 ESTABLISHMENT OF PRIMARY GLIOBLASTOMA CELL CULTURES FOR NEW TREATMENT APPROACHES SCREENING. RENATO JOSÉ DA SILVA OLIVEIRA, OLGA MARTINHO, CARLOS CLARA, JOSÉ REYNALDO ALMEIDA, RUI MANUEL REIS B - 20 DNA REPAIR ENZYMES ON PLASMIDS EXPOSED TO LOW-INTENSITY INFRARED LASER. KEILA DA SILVA CANUTO, ROBERTA DA SILVA MARCIANO, LUIZ PHELIPPE DA SILVA SERGIO, OSCAR ROBERTO GUIMARÃES, GIOVANNI AUGUSTO CASTANHEIRA POLIGNANO, MAURO GELLER, FLAVIA DE PAOLI, ADENILSON DE SOUZA DA FONSECA B - 21 CELLULAR BEHAVIOR OF PROSTATE CANCER IN AN INFLAMMATORY MICROENVIRONMENT. AMADO ALFREDO QUINTAR, CAROLINA LEIMGRUBER, MARIANA MACCIONI, ANDREAS DOLL, CRISTINA ALICIA MALDONADO B - 22 ANTITUMOR EFFECT OF RHODIUM(II) CITRATE-LOADED MAGNETIC NANOPARTICLES IN MICE BEARING BREAST CANCER. SÔNIA NAIR BÁO, ANA LUISA MIRANDA VILELA, RICARDO GUIRELLI SIMÕES DE OLIVEIRA, LUÍS AUGUSTO M. TELLES, SÔNIA NAIR BÁO B - 23 RESVERATROL AND QUERCETIN INDUCE SENESCENCE-LIKE GROWTH ARREST IN GLIOMA CELLS BY INCREASING DNA DAMAGE. LAUREN LUCIA ZAMIN, EDUARDO C. FILIPPI-CHIELA, ALESSANDRA PELEGRINI, CHRISTIANNE SALBEGO, GUIDO LENZ B -24 ANALYSIS OF THE CYTOTOXIC POTENTIAL OF JUÇARA EXTRACTS (EUTERPE OLERACEA MART.) IN HUMAN MALIGNANT CELLS. DULCELENA FERREIRA SILVA, MARIA DO DESTERRO SOARES BRANDÃO NASCIMENTO, FLÁVIA CASTELLO BRANCO VIDAL, JOSÉ ANDRÉS MORGADO DÍAZ, SIMONE FERNANDES, PRISCILA DANTAS, DÉBORA SANTOS, MARIA CÉLIA PIRES COSTA, WALBERT EDSON MUNIZ FILHO, ROBERTO SOARES DE MOURA B - 25 MYOSIN II EXPRESSION AND REGULATION ON ORAL SQUAMOUS CELL CARCINOMA. OTÁVIO FRANCISCO GOMES DIAS, BERNARDO SALIM SILVEIRA, ALESSANDRA MAGNUSSON, ISABEL DA SILVA LAUXEN, MANOEL SANT'ANA FILHO, MARCELO LAZZARON LAMERS B - 26 FINASTERIDE TREATMENT DOWNREGULATES FIBRONECTININDUCED MMP-2 AND MMP-9 ACTIVITIES IN HUMAN PROSTATIC EPITHELIAL CELLS. ANDREI MOROZ, FLAVIA KARINA DELELLA, ELENICE DEFFUNE, SÉRGIO LUIS FELISBINO B - 27 IN VITRO ANTITUMOR ACTIVITY OF EXTRACTS FROM ENDOPHYTIC FUNGI ASSOCIATED WITH CLUSIA SP. ANTONIO CESAR CORRÊA SILVA FILHO, LUIZ HENRIQUE ROSA, LUCIANA MARIA SILVA B - 28 CYTOTOXIC ACTIVITY OF PYRANONAPHTHOQUINONES AGAINST TWO DIFFERENT LEUKEMIA CELL LINES. PATRÍCIA CRISTINA RODRIGUES, SABRINA BAPTISTA FERREIRA, VITOR FRANCISCO FERREIRA, FLORIANO PAES SILVA JUNIOR B - 29 INTRACELLULAR OXIDATIVE STRESS IS ASSOCIATED WITH NONSMALL CELL LUNG CANCER HUMAN CELL LINES AGGRESSIVENESS. FERNANDA STAPENHORST FRANÇA, LEONARDO LISBOA MOTTA, JULIANE BORBA MINOTTO, MATHEUS BECKER FREITAS, GUILHERME ANTÔNIO BEHR, ALFEU ZANOTTO-FILHO, MELISSA MEDEIROS MARKOSKI, JOSÉ CLÁUDIO FONSECA MOREIRA, FÁBIO KLAMT B - 30 SUPEROXIDE ANION MODULATES DNMT1 LEVELS VIA RAS/MEK/ERK PATHWAY DURING MELANOCYTE MALIGNANT TRANSFORMATION ASSOCIATED WITH SUSTAINED STRESS CONDITION. FERNANDA MOLOGNONI, FABIANA HENRIQUES MACHADO DE MELO, TIAGO FRANCO DE OLIVEIRA, ANA PAULA DE MELO LOUREIRO, MIRIAM GALVONAS JASIULIONIS B - 31 LOW-INTENSITY RED LASER DEACRESES SURVIVAL OF PLASMIDS IN ESCHERICHIA COLI PROFICIENT AND DEFICIENT ON DNA REPAIR CELLS. ROBERTA DA SILVA MARCIANO, LUIZ PHILIPPE DA SILVA SERGIO, OSCAR ROBERTO GUIMARÃES, GIOVANNI AUGUSTO CASTANHEIRA POLIGNANO, MAURO GELLER, FLAVIA DE PAOLI, ADENILSON DE SOUZA DA FONSECA B - 32 HPV INFECTION CORRELATION IN THE PATHOGENESIS OF CERVICAL CANCER. KEILA ALVES DA SILVA, SORAYA LOBATO, ANNAMARIA RAVARA VAGO B - 33 IN VITRO ANTITUMOR ACTIVITY OF SYNTHETIC SCHIFF BASES IN HUMAN CELL LINES. MARIANA DE PAULA LAZAROTTI, JOSIANE BARBOSA PIEDADE, CLEITON MOREIRA DA SILVA, LEANDRO NUNES SAMPAIO, ANGELO DE FÁTIMA, LUCIANA MARIA SILVA B - 34 CYTOTOXICITY OF SYNTHETIC ANALOGS OF VISCOSALINE AND THEONELLADIN C. JULIANA REY CANUTO SANT”ANA PEREIRA, ALINE BRITO DE LIMA, GUSTAVO HENRIQUE RIBEIRO VIANA, LUCIANA MARIA SILVA, FERNANDO DE PILLA VAROTTI B - 35 THE ROLE OF GPC3 IN CELL PROLIFERATION IN CELL LINES OF RENAL CARCINOMA. MARINA CURADO VALSECHI, ANA BEATRIZ BORTOLOZO DE OLIVEIRA, MARÍLIA DE FREITAS CALMON, PAULA RAHAL B - 36 BIOLOGICAL EFFECTS OF MAGNETIC NANOPARTICLES: STUDIES IN VITRO AND IN VIVO FOR POTENTIAL STRATEGIES IN ORAL CARCINOMA. NATALIA MARIA CANDIDO, MARILIA DE FREITAS CALMON, ARYANE TOFANELLO SOUZA, SEBASTIÃO ROBERTO TABOGA, JOSÉ GERALDO NERY, PAULA RAHAL B - 37 ANTI-MRP1 ACTIVITY OF 3B-ACETYL TORMENTIC ACID, A PENTACICLIC TRITERPENE FROM CECROPIA LYRATILOBA. GLEICE DA GRAÇA ROCHA, RODRIGO RODRIGUES DE OLIVEIRA, MARIA A.C. KAPLAN, CERLI ROCHA GATTASS B - 38 ANTITUMOR ACTIVITY OF POMOLIC ACID IN GLIOBLASTOMA MULTIFORME CELL LINE. LÍVIA PAES TAVARES PACHECO GUIMARÃES, CERLI ROCHA GATTASS B - 39 EPITHELIAL-MESENCHYMAL TRANSITION IN CERVIX CARCINOMA: DONWREGULATION OF E-CADHERIN IN INVASION FRONT. PRISCILA SAMARA SARAN, SILVIA VANESSA LOURENÇO, CLÁUDIA MALHEIROS COUTINHO-CAMILLO, FERNANDO AUGUSTO SOARES B - 40 TUMORSPHERE GROWTH AND CANCER STEM CELL POPULATION ARE REDUCED BY EXTRACELLULAR ATP IN GLIOBLASTOMA CELLS. PÍTIA FLORES LEDUR, EMILLY SCHLEE VILLODRE, GUIDO LENZ B - 41 ANTI-INFLAMMATORY PROTEIN ANNEXIN-1 AND FORMYL PEPTIDE RECEPTOR-2 AS THERAPEUTIC TARGETS FOR HUMAN LARYNX CANCER. THAÍS SANTANA GASTARDELO, LUCAS RIBEIRO DE AZEVEDO, FLÁVIA CRISTINA RODRIGUES LISONI, BIANCA DA CUNHA RODRIGUES, ELOIZA HELENA SILVA TAJARA, SÉRGIO LUIS RAPOSO, JOSÉ VICTOR MANIGLIA, PATRÍCIA MALUF CURY, SONIA MARIA OLIANI B - 42 EVALUATION OF LANGERHANS CELLS DENSITY AND HPV INFECTION IN CERVICAL SQUAMOUS INTRAEPITHELIAL LESIONS AND INVASIVE CANCER. DANIELE DE SOUZA CAMARGOS, ALEXANDRE TAFURI, PAULA ÁVILA FERNANDES, MARCELO VIDIGAL CALLIARI, MARCOS XAVIER SILVA, ANNAMARIA RAVARA VAGO B - 43 CELLULAR PRION-HEAT-SHOCK ORGANIZING PROTEIN INTERACTION AS A NOVEL THERAPEUTIC TARGET FOR GLIOBLASTOMAS. LOPES, M.H, QUEIROZ-HAZARBASSANOV N.G.T, RODRIGUES, B. R., SANTOS, T. G., CUNHA I.W., OBA-SHINJO, S.M, MARIE, S.K.N., MARTINS, V.R. B - 44 COMPARATIVE ANTITUMOR EFFECT OF DIGITALIS ON CERVIX AND COLON CANCER CELL LINES. SAYONARAH CARVALHO ROCHA, LUIZA DAL-RIOS NEVES, MARCO TULIO CORREA PESSOA, SILMARA LUCIA GREGO ALVES, ISABELLA VIANA SILVA, LUCIANA MARIA DA SILVA, JOSÉ AUGUSTO FP VILLAR, FABIO VIEIRA DOS SANTOS, FERNANDO DE PILLA VAROTTI, LEANDRO AUGUSTO BARBOSA B - 45 THE INFLUENCE OF O-GLCNACYLATION IN THE MOTILITY OF ALVEOLAR EPHITHELIAL CANCER CELLS. JOANA LAUREANO DONADIO, ANA CLARA BRANDÃO MEDINA DOLHER SOUZA, PATRÍCIA DE CARVALHO CRUZ, LEONARDO FREIRE-DE-LIMA, ADRIANE REGINA TODESCHINI, WAGNER BARBOSA DIAS B - 46 GENETICS OF GLIOMA-ROLE OF A RNA BINDING PROTEIN IN MALIGNANCY. KUMAR SOMASUNDARAM B - 47 ANTINEOPLASTIC EFFECT OF TWO DIFFERENT APPROACHES OF LQB-118 ADMINISTRATION IN MURINE MELANOMA MODEL. EDUARDO SALUSTIANO JESUS DOS SANTOS, GABRIEL GONÇALVES DA SILVA SANTOS, MATHEUS LOURENÇO DUMAS, ALCIDES JOSÉ MONTEIRO DA SILVA, PAULO ROBERTO RIBEIRO COSTA, VIVIAN MARY BARRAL DODD RUMJANEK 91 B -48 LECTIN OBTAINED OF CAULIFLOWER (BRASSICA OLERACEA VAR. BOTRITYS) INHIBITS PROLIFERATION OF CELL LINEAGE OF BREAST CANCER. MONISE VIANA ABRANCHES, LORENA NACIF MARÇAL, NATÁLIA CRISTINA SANTOS COSTA, SÍLVIA ALMEIDA CARDOSO, SÉRGIO OLIVEIRA DE PAULA, LEANDRO LICURSI DE OLIVEIRA B - 49 EFFECT OF EPIGENETIC DRUGS IN ANTI-HORMONAL TREATMENT OF BREAST TUMOR CELL LINES. DANIELA FILIPPINI IERARDI, GIMENA AGUIAR, MIRIAM GALVONAS JASIULIONIS B - 50 SURVIVAL PROGENY DERIVED FROM IRRADIATED PARENTAL COLORECTAL CANCER CELLS: MORFOLOGICAL AND FUNCTIONAL ANALYSIS. PRISCILA GUIMARÃES DE MARCONDES, LÍLIAN GONÇALVES BASTOS, JOSÉ ANDRÉS MORGADO DÌAZ B - 51 ANALYSIS OF CLASSIC CADHERINS EXPRESSION IN A MURINE MELANOMA MODEL. LORENA NACIF MARÇAL, ROSEMAIRY LUCIANE MENDES, MARCELO LOBATO MARTINS, ADILSON ARIZA ZACARO B - 52 RETINOBLASTOMA (RB) PROTEIN KNOCK-DOWN INCREASES APOPTOSIS AND REDUCES ACID VESICLES FORMATION IN GLIOBLASTOMA ETOPOSIDE TREATED CELLS. DEBORAH BIASOLI, SUZANA ASSAD KHAN, TAIS AZEVEDO, VIVALDO MOURA-NETO, HELENA LOBO BORGES B - 53 TRANSGLUTAMINASE 2 ACTIVATES BOTH CASPASE-DEPENDENT AND CASPASE-INDEPENDENT APOPTOTIC CELL DEATH VIA CALPAIN/BAX SIGNALING PATHWAY IN RESPONSE TO PHOTODYNAMIC THERAPY. JE-OK YOO, YOUNG-CHEOL LIM, YOUNG-MYEONG KIM, KWON SOO HA B - 54 MINICHROMOSOME MAINTENANCE 7 PROTEIN IS A RELIABLE BIOLOGICAL MARKER FOR HUMAN CERVICAL PROGRESSIVE DISEASE. SORAYA LOBATO, ALEXANDRE TAFURI, PAULA ÁVILA FERNANDES, MARCELO VIDIGAL CALIARI, MARCOS XAVIER SILVA, MARCELO ANTÔNIO PASCOAL XAVIER, ANNAMARIA RAVARA VAGO B - 55 IMMUNOMODULATORY AND ANTINEOPLASTIC EFFECT OF LQB118, A PTEROCARPANOQUINONE, IN VIVO. VIVIAN MARY BARRAL DODD RUMJANEK, EDUARDO SALUSTIANO JESUS DOS SANTOS, ALCIDES JOSE MONTEIRO DA SILVA, PAULO ROBERTO RIBEIRO COSTA, VIVIAN MARY BARRAL DODD RUMJANEK B - 56 TARGETING METNASE TO ENHANCE CHEMOTHERAPY. ANDREI LEITÃO, ELIZABETH A. WILLIAMSON, LEAH DAMIANI, CHELIN HU, HELEN HATHAWAY, TUDOR I. OPREA, LARRY SKLAR, MONTASER SHAHEEN, JULIE BAUMAN, WEI WANG, JAC A. NICKOLOFF, SUK-HEE LEE, ROBERT HROMAS B - 57 THE ROLE OF GLUTAMINASE-INTERACTING PROTEIN AND LIVERTYPE GLUTAMINASE ASSOCIATION ON CELL REDOX HOMEOSTASIS. ROBERTA CASAGRANDE SAEZ, KALIANDRA DE ALMEIDA GONÇALVES, SANDRA MARTHA GOMES DIAS B - 58 VEGFR-3 EXPRESSION IN CERVICAL CARCINOMA AS A BIOMARKER OF TUMOR RESPONSE TO RADIOCHEMOTHERAPY. IOANA-CARMEN BRIE, VIORICA NAGY, NICOLAE TODOR, RARES BUIGA, OVIDIU BALACESCU, LUMINITA LELUTIU, CLAUDIA ORDEANU, EVA FISCHER-FODOR B - 59 CALLUNA VULGARIS INDUCES APOPTOSIS AND INHIBITS INVASION IN A431 HIGHLY METASTATIC EPIDERMOID CARCINOMA CELL LINE. MARIA PERDE-SCHREPLER, EVA FISCHER FODOR, CORINA TATOMIR, TIBOR KRAUSZ, CALIN PRECUP B - 60 EFFECT OF THE COMPOUND A2CN ON THE CYTOTOXITY IN LEUKEMIC CELL LINES. CAIO CÉSAR BARBOSA BOMFIM, GLÁUCIA VERÍSSIMO FAHEINA MARTINS, BRUNA BRAGA DANTAS, ALETHÉIA LACERDA DA SILVEIRA, CLAUDIO GABRIEL LIMA JUNIOR, MÁRIO LUIZ ARAUJO DE ALMEIDA VASCONCELLOS, DEMETRIUS ANTONIO MACHADO DE ARAÚJO B - 61 IDENTIFICATION OF NOVEL MOLECULAR MARKERS IN HUMAN GLIOBLASTOMA WITH THERAPEUTIC POTENTIAL. LAURA BEATRIZ DA SILVA CARDEAL, ALINNE LE FOSSE, JUSSARA MICHALOSKI, RAFAEL MALAGOLI, RICARDO JOSE GIORDANO B -66 LYCOPENE AND BETA-CAROTENE INDUCE GROWTH-INHIBITORY AND PROAPOPTOTIC EFFECTS ON PITUITARY TUMOR CELLS. NATÁLIA FERREIRA HADDAD, ANDERSON JUNGER TEODORO, FELIPE LEITE DE OLIVEIRA, NATHÁLIA SOARES, RÔMULO MEDINA DE MATTOS, RÔMULO DEZONNE, FLÁVIA C. ALCÂNTARA GOMES, MÔNICA ROBERTO GADELHA, LUIZ EURICO NASCIUTTI, LEANDRO MIRANDA-ALVES B - 67 EFFECT FROM A POLYSACHARIDE FROM SARGASSUM VULGARE IN ENDOTHELIAL AND TUMORAL CELL LINES: A NEW STRATEGY FOR ANTITUMOR AND ANTIANGIOGENIC. CELINA MARIA PINTO GUERRA DORE, MONIQUE GABRIELA DAS CHAGAS FAUSTINO ALVES, TIAGO GOMES COSTA, MARÍLIA SILVA DO NASCIMENTO, HUGO WESCLEY BARROS ALMEIDA, ALMINO AFONSO DE OLIVEIRA PAIVA, LUCIANA GUIMARÃES ALVESFILGUEIRA, EDDA LISBOA LEITE B - 68 EGF MODULATES CLAUDIN EXPRESSION INCREASING THE TUMORIGENIC POTENTIAL IN COLORECTAL CANCER CELLS. NATALIA FORTUNATO DE MIRANDA, WALDEMIR FERNANDES DE SOUZA, BRUNO KAUFMANN ROBBS, JOÃO PAULO DE BIASO VIOLA, JOSÉ ANDRÉS MORGADO DÍAZ B - 69 SHH SIGNALING PATHWAY IN MODULATING GBM PROLIFERATION. TANIA CRISTINA LEITE DE SAMPAIO E SPOHR, INGRID ROSENBURG CORDEIRO, JOSÉ MARQUES BRITO NETO, VIVALDO MOURA NETO B - 70 DIFFERENTIALLY EXPRESSED STEM CELL MARKERS IN BREAST CANCER STEM CELLS. ALINE RAMOS MAIA LOBBA, MARIA FERNANDA FORNI, ANA CLAUDIA OLIVEIRA CARREIRA, MARI CLEIDE SOGAYAR B - 71 ROLES OF NUP98 IN GENE EXPRESSION REGULATION AND ITS LINKS TO CARCINOGENESIS. JULIANA S. CAPITANIO, RICHARD W. WOZNIAK B - 72 IN VITRO ANTICANCER ACTIVITY OF N-GLYCAN BIOSYNTHESIS INHIBITORS IN COLORECTAL CANCER CELLS. CARLOS ALBERTO FREIRE NETO, JULIO CESAR MADUREIRA DE FREITAS JUNIOR, JOSÉ ANDRES MORGADO-DÍAZ B - 73 SEX HORMONES EFFECTS ON ADAMTS-1 LEVELS IN NORMAL AND TUMORAL BREAST CELLS. SUÉLY VIEIRA DA SILVA, SHEILA CRISTINA ROSAS DE ARGÔLO, EMERSON SANTOS, VANESSA MORAIS FREITAS B - 74 LAMININ-DERIVED PEPTIDES C16 AND AG73 REGULATE EXPRESSION LEVELS OF SPOCK-1 AND MT1-MMP IN BREAST CANCER CELLS. BASILIO SMUCZEK, EMERSON DE SOUZA SANTOS, VANESSA M. DE FREITAS, RUY GASTALDONI JAEGER B - 75 IDENTIFICATION OF CRUDE EXTRACT MOLECULES FROM BEANS INVOLVED IN SKIN CANCER PREVENTION. GRAZIELLA ANSELMO JOANITTI, EDUARDO FERNANDES BARBOSA, LUCIANO PAULINO SILVA, RICARDO BENTES AZEVEDO, SONIA MARIA DE FREITAS B - 76 PTEN OVEREXPRESSION REVERTS THE MALIGNANT PHENOTYPE OF COLORECTAL CANCER CELLS IN AN EVENT MEDIATED BY THE WNT/B-CATENIN PATHWAY. WALLACE MARTINS DE ARAÚJO, PEDRO DANIEL SILVA DE MORAES, BRUNO KAUFMANN ROBBS, JOÃO PAULO DE BIASO VIOLA, JOSÉ ANDRES MORGADO-DÍAZ B - 77 EFFECTS OF HCG AND DERIVETED-ANGIOTENSIN PEPTIDES ON CELL VIABILITY AND APOPTOSIS IN TUMORAL (MCF-7) AND NORMAL (MCF10A) EPITHELIAL BREAST CELLS. CORREA-NORONHA, SAA, NORONHA, SMR, ROZENCHAN, PB, BERNARDO, W, SHIMUTA, SI, NAKAIE, CR, GEBRIM, LH, NAZARIO, ACP, SILVA, IDCG B - 78 ATYPICAL FUNCTIONS OF THE CELL CYCLE INHIBITOR P21WAF1/CIP1: A POSSIBLE ROLE IN SURVIVAL OF MELANOMA CELLS. GABRIELA NANA COLANERI, ADRIANA TAVEIRA DA CRUZ, ROBERTA SESSA STILHANO, SANG WON HAN, MIRIAM GALVONAS JASIULIONIS B - 79 LPA INDUCES CELL PROLIFERATION THROUGH A RHO-ROCK SIGNALING IN HCT-116 CELLS. RUBEM JOSÉ PERES MOREIRA, FERNANDA LEVE, JOSÉ ANDRÉS MORGADO-DÍAZ B - 80 STEPS TOWARD NOVEL ANTI-VEGF THERAPEUTIC AGENTS. JUSSARA MICHALOSKI SOUZA, RICARDO JOSÉ GIORDANO B - 62 A CASE OF DISSEMINATED NEOPLASIA IN MANGROVE OYSTERS CRASSOSTREA GASAR. NATANAEL DANTAS FARIAS, FERNANDO RAMOS QUEIROGA, HELENE HÉGARET, PHILIPPE SOUDANT, LUIS FERNANDO MARQUES SANTOS, PATRÍCIA MIRELLA DA SILVA SCARDUA B - 81 DIFFERENTIAL PROTEINS EXPRESSION IN CANCER CELL LINES SKBR3 AND MCF-7 BY 17 BETA-ESTRADIOL (E2), ICI 182,780 AND G1. MILENE SCHMIDT LUNA, ANDRÉIA DE SOUZA, CINTIA SCUCUGLIA HELUANY, CATARINA SEGRETI PORTO, NORMA YAMANOUYE B - 63 MORPHOLOGICAL FEATURES OF THE MELANOMA B16F10 AND B16F0 CELL LINES AND TUMORS. JULIANNA MARIA DA CUNHA DE OLIVEIRA SANTOS, EULÓGIO CARLOS CARVALHO, FÁBIO LOPES OLIVARES, WILLIAM RODRIGUES FREITAS, LAYLA JANAÍNA HISSA BORGES, MILTON KANASHIRO, LUIS OTTONIEL LATORRE, CAMILA CRUZ RIBEIRO, ARNOLDO ROCHA FAÇANHA B - 82 APOPTOSIS INDUCTION BY PHORBOL ESTER-RESPONSIVE PKC ISOFORMS IN HUMAN EMBRYONIC KIDNEY CELL LINES (HEK 293) DISPLAYING A RAS-DEPENDENT MALIGNANT PHENOTYPE. JULIANA GALVÃO DA SILVA, HUGO AGUIRRE ARMELIN B - 64 IN VITRO STUDY OF ASSOCIATION OF NATURAL COMPOUNDS AND CHEMOTHERAPY. AMANDA VALLE PINHATTI, ANA ABUJAMRA, FRANCISCO MAIKON CORRÊA DE BARROS, CAROLINE FARIAS BRUNETTO, GILBERTO SCHWARTSMANN, GILSANE VON POSER B - 65 CYTOPLASMIC DISTRIBUTION OF KAISO IN BLAST CRISIS OF CHRONIC MYELOID LEUKEMIA. GREYCE CHRISTINE LISBOA BUENO, JAIME COFRE, PIETRO LENTZ MARTINS CANTÚ, JOÃO RICARDO LACERDA DE MENEZES, ELIANA ABDELHAY, LUCIANA PIZZATTI B - 83 THE EFFECT IN VITRO OF ANTI-INFLAMMATORY PROTEIN ANNEXIN A1 ON TUBULOGENESIS AND CELL ADHESION. JÉSSICA ZANI LACERDA, THAÍS SANTANA GASTARDELO, CARINE CRISTIANE DREWES, SANDRA HELENA POLISELLI FARSKY, SONIA MARIA OLIANI B - 84 EVALUATION OF CELL ADHESION IN BREAST AND LARYNGEAL CANCER CELLS WITH PHOTODYNAMIC THERAPY. GEISA NOGUEIRA SALLES, JULIANA MACEDO COSTA COUCEIRO, CRISTINA PACHECO SOARES 92 B - 85 CONDITIONED MEDIA FROM EPITHELIAL OVARIAN CANCER CELL LINES CHRONICALLY EXPOSED TO CISPLATIN IS CAPABLE OF INDUCING APOPTOSIS IN NAIVE CELLS. ALICE LASCHUK HERLINGER, CELSO CARUSO NEVES, LETICIA BATISTA AZEVEDO RANGEL B - 86 SUPER-EXPRESSION OF RAS AND SILENCE OF SYNDECAN-4 IS RELATED WITH TUMORIGENESIS. RENAN PELLUZZI CAVALHEIRO, THAIS RUEGGER JARROUGE-BOUÇAS, RODRIGO IPPOLITO BOUÇAS, GABRIEL LOPES ARGELLO CUNHA, VIVIEN JANE COULSON-THOMAS, TARSIS GESTEIRA FERREIRA, EDUARDO HENRIQUE CUNHA DE FARIAS, MARCELO ANDRADE DE LIMA, EDVALDO DA SILVA TRINDADE, EDGAR JULIAN PAREDES-GAMERO, JULIANA LUPORINI DREYFUSS, CARLA CRISTINA LOPES DE AZEVEDO, HELENA BONCIANI NADER LÍVIA CARVALHO FERREIRA, JULIANA RAMOS LOPES, MARINA GOBBE MOSCHETTA, DEBORA AP. PIRES DE CAMPOS ZUCCARI B - 102 TESTING PHAGE CLONES FOR IMPROVE THYROID CANCER DIAGNOSTIC. CAROLINA FERNANDES REIS, PATRICIA TIEME FUJIMURA, FABIANA DE ALMEIDA ARAÚJO SANTOS, JOÃO PAULO BORGES, CARLOS UEIRA VIEIRA, LUIZ RICARDO GOULART, LAURA STERIAN WARD B - 103 POTENTIAL PROGNOSTIC VALUE OF CA 15-3 IN MAMMARY CANCER. GABRIELA BOTTARO GELALETI, CAMILA LEONEL, MARINA GOBBE MOSCHETTA, BRUNA VICTORASSO JARDIM, LARISSA BAZELA MASCHIO, LÍVIA CARVALHO FERREIRA, JULIANA RAMOS LOPES, THAIZ FERRAZ BORIN, NAIANE DO NASCIMENTO GONÇALVES, DEBORA APARECIDA PIRES DE CAMPOS ZUCCARI B - 87 ATP - INDUCED CELL DEATH BY P2X7 RECEPTOR IN HUMAN CERVICAL CARCINOMA CELL LINE. PAOLA DE ANDRADE MELLO, EDUARDO C. FILIPPI-CHIELA, ALINE BECKENKAMP, DANIELLE SANTANA BERTODO, JESSICA NASCIMENTO, LUCIANA N. CALIL, EMERSON CASALI, ALESSANDRA NEJAR BRUNO, JULIANO PACCEZ, LUIZ FERNANDO ZERBINI, MÁRCIA R. WINK, GUIDO LENZ, ANDRÉIA BUFFON B - 104 PROLIFERATIVE EFFECTS OF POD1/TCF21 TRANSCRIPTION FACTOR IN HUMAN ADRENOCORTICAL TUMOR CELL CULTURES. MONICA MALHEIROS FRANÇA, MARIZA GERDULO SANTOS, BRUNO FERRAZ DE SOUZA, ANTONIO M. LERARIO, MARIA CANDIDA FRAGOSO, ANA CLAUDIA LATRONICO, BERENICE B. MENDONÇA, CLAUDIMARA FERINI PACICCO LOTFI B - 88 PROGRESSION OF HUMAN GLIOBLASTOMA IN THE BRAIN PARENCHYMA FROM IMMUNOCOMPETENT MICE. CELINA GARCIA DA FONSECA, LUIZ HENRIQUE MEDEIROS GERALDO, LUIZ GUSTAVO DUBOIS, FERNANDA TOVARMOLL, JOÃO MENEZES, VIVALDO MOURA NETO, FLAVIA REGINA SOUZA LIMA B - 105 EFFECTS OF BRAZILIAN PALM TREE EXTRACT ON ACTH-SECRETING MOUSE PITUITARY TUMOR CELLS. FLÁVIA MOSCARDINI, NATALIA FERREIRA HADDAD, MARIA APARECIDA DE OLIVEIRA DOMINGOS, LÚCIO MENDES CABRAL, LEANDRO MIRANDA-ALVES, LUIZ EURICO NASCIUTTI B - 89 HUMAN GLIOBLASTOMA CANCER STEM CELLS ARE SENSITIVE TO DOXORUBICIN AND TEMOZOLOMIDE. EMILLY SCHLEE VILLODRE, PATRÍCIA LUCIANA DA COSTA LOPEZ, GUIDO LENZ B - 106 ROLE OF CANCER STEM CELLS IN GLIOBLASTOMA INVASION OF IN THE BRAIN PARENCHYMA. FERNANDO DOS SANTOS ASSUNÇÃO, GABRIELA BASILE CARBALLO, GRASIELLA MARIA VENTURA MATIOSZEK, CHARLES VARGAS LOPES, CAROLINE MOREIRA, CELINA GARCIA, HERVÉ CHNEIWEISS, ROGERIO ARENA PANIZZUTTI, SUZANA ASSAD KAHN, VIVALDO MOURA NETO B - 90 THE ROLE OF P53 IN THE TUMOR-MICROENVIRONMENT INTERACTION. MORGANA FERREIRA SOBRINHO, DEBORAH BIASOLI, DYANNA GALAXE DE MATOS, VIVALDO MOURA NETO, HELENA LOBO BORGES, FLAVIA REGINA SOUZA LIMA B - 91 BAUHINIA FORFICATA LECTIN (BFL) INDUCES MITOCHONDRIAL CELL DEATH AND INTEGRIN-MEDIATED ANTI-ADHESION ON MCF-7 HUMAN BREAST CANCER CELLS. MARIANA CRISTINA CABRAL SILVA, CLÁUDIA ALESSANDRA ANDRADE DE PAULA, JOANA GASPERAZZO FERREIRA, EDGAR JULIAN PAREDESGAMERO, RODRIGO DA SILVA FERREIRA, MISAKO UEMURA SAMPAIO, MARIA TEREZA DOS SANTOS CORREIA, MARIA LUIZA VILELA OLIVA B - 92 MOLECULAR MARKER ANALYSIS IN HEAD AND NECK SQUAMOUS CELL CARCINOMA. ELAINE STUR, ELDAMÁRIA DE VARGAS WOLFGRAMM, LIDIANE PIGNATON AGOSTINI, LUCAS LIMA MAIA, LYVIA NEVES REBELLO ALVES, IÚRI DRUMOND LOURO B - 93 INCIDENCE OF HUMAN PAPILOMAVIRUS IN HEAD AND NECK SQUAMOUS CELL CARCINOMA IN ESPIRITO SANTO, BRASIL. LIDIANE PIGNATON AGOSTINI, MARIANA PENHA DE NADAI SARTORI, ELAINE STUR, ELDAMÁRIA DE VARGAS WOLFGRAM, IÚRI DRUMOND LOURO B - 94 PROTECTIVE ACTION IN NORMAL CELLS AND APOPTOSIS IN HUMAN LIVER CANCER CELLS BY THE PHYLLANTHUS NIRURI DRY EXTRACT. HUGO GONÇALO GUEDES, JÉSSICA AQUINO VILAÇA, ANA LUIZA CABRAL DE SÁ, AURIGENA ANTUNES DE ARAÚJO, GERLANE COELHO BERNARDO GUERRA, RAIMUNDO FERNANDES DE ARAÚJO JÚNIOR B - 107 C-MYC EXPRESSION IN DYSPLASIAS AND CARCINOMAS DEVELOPED IN THE TONGUE FROM GALECTIN-3-DEFICIENT AND WILD-TYPE MICE CHALLENGED BY 4NQO. GUSTAVO JOSÉ DE MORAIS GONÇALVES, WANDERSON DE ALMEIDA RAMOS, ROGER CHAMMAS, ADRIANO MOTA LOYOLA, SERGIO VITORINO CARDOSO, PAULO ROGÉRIO DE FARIA B - 108 DIFFERENTIAL EXPRESSION AND ACTIVITY OF THE CD26/DPPIV IN HUMAN CERVICAL CARCINOMA CELLS. ALINE BECKENKAMP, DANIELLE BERTODO SANTANA, JÉSSICA NASCIMENTO, CAROLINE GUERRA MARANGON, JULIANO PACCEZ, LUIZ FERNANDO ZERBINI, MÁRCIA ROSÂNGELA WINK, ALESSANDRA NEJAR BRUNO, ANDRÉIA BUFFON B - 109 PHOSPHATIDIC ACID INCREASED BY LPP INHIBITION INDUCES LIGAND-INDEPENDENT EGFR ENDOCYTOSIS INVOLVING ACTIVATION OF MAPKS. CLAUDIA METZ, JUAN JUNG, CAROLINA OTERO, ANDREA SOZA, ALFONSO GONZÁLEZ B - 110 REGULATION OF FOCAL ADHESION KINASE ACTIVITY IN ORAL SQUAMOUS CELL CARCINOMA. BERNARDO SALIM SILVEIRA, OTAVIO FRANCISCO GOMES DIAS, ALESSANDRA MAGNUSSON, ISABEL DA SILVA LAUXEN, MANOEL SANT’ANA FILHO, MARCELO LAZZARON LAMERS B - 111 PHYLLANTHUS NIRURI EXTRACTS AND CISPLATIN HAVE GROWTH INHIBITORY EFFECTS ON CANCER CELL LINES. JESSICA AQUINO VILAÇA, RAIMUNDO FERNANDES DE ARAÚJO JÚNIOR B - 95 PROGNOSTIC EVALUATION OF S100A4 AND P53 IN CERVICAL CANCER CELLS. JÉSSICA NASCIMENTO, REGINA BIASIBETTI, PATRÍCIA NARDIN, ALINE BECKENKAMP, DANIELLE BERTODO SANTANA, ALESSANDRA NEJAR BRUNO, LUCIANE CALIL, MARIA ISABEL A. EDELWEISS, CARLOS ALBERTO GONÇALVES, ANDRÉIA BUFFON B - 112 MECHANISMS OF FGF2 TOXICITY IN RAS-DRIVEN MALIGNANT CELLS: CELL DIVISION BLOCKAGE AND PROTEOTOXIC STRESS. MATHEUS HENRIQUE DOS SANTOS DIAS, FÁBIO NAKANO, CECÍLIA SELLA FONSECA, ANDRÉ ZELANIS PALITOT PEREIRA, SOLANGE MARIA DE TOLEDO SERRANO, HUGO AGUIRRE ARMELIN B - 96 ROLE OF DLG5 IN PROSTATE CANCER PROGRESSION. LUCIA TOMIYAMA, TAKUHITO SEZAKI, KAZUMITSU UEDA, NORIYUKI KIOKA B - 113 EGF PROMOTES CELL MIGRATION IN LUNG CANCER CELL LINES. CAMILA LAUAND, PAULA REZENDE TEIXEIRA, EVANDRO LUÍS DE OLIVEIRA NIERO, GLÁUCIA MARIA MACHADO SANTELLI B - 97 THE ROLE OF LYSOPHOSPHATIDIC ACID IN THE MICROGLIAGLIOBLASTOMA INTERACTION. RACKELE FERREIRA DO AMARAL, TANIA CRISTINA LEITE DE SAMPAIO E SPOHR, FABIO DE ALMEIDA MENDES, VIVALDO MOURA NETO, FLAVIA REGINA SOUZA LIMA B - 98 INTERACTION OF TWO DISINTEGRINS WITH BREAST TUMOR CELLS. ARACELI CRISTINA DURANTE, LÍVIA MARA SANTOS, HERNANDES FAUSTINO DE CARVALHO, EDWARD SHAW, CHARLOTTE LEDBETTER OWNBY, HELOÍSA SOBREIRO SELISTRE-DE-ARAÚJO B - 99 MOLECULAR CLONING AND EXPRESSION OF ALTERNAGIN-C, A DISINTEGRIN FROM RHINOCEROPHIS ALTERNATUS VENOM. LIVIA MARA SANTOS, VERÔNICA ASSALIN ZORGETTO, MÔNICA ROSAS DA COSTA IEMMA, ARACELI CRISTINA DURANTE, DULCE HELENA FERREIRA DE SOUZA, HELOISA SOBREIRO SELISTRE DE ARAÚJO B - 100 EVALUATION OF BREAST CANCER CELL LINES VIABILITY IN RESPONSE TO TREATMENT WITH MELATONIN. JULIANA RAMOS LOPES, BRUNA VICTORASSO JARDIM, LARISSA BAZELA MASCHIO, THAIZ FERRAZ BORIN, LÍVIA CARVALHO FERREIRA, NAIANE DO NASCIMENTO GONÇALVES, CAMILA LEONEL, MARINA GOBBE MOSCHETTA, GABRIELA BOTTARO GELALETI, DEBORA AP. PIRES DE CAMPOS ZUCCARI B - 101 GSH AND GSH-PX EXPRESSION IN PRIMARY CULTURE CELL OF CANINE MAMMARY TUMORS AFTER EXPOSURE TO DOXORUBICIN. CAMILA LEONEL DA SILVA, GABRIELA BOTTARO GELALETI, BRUNA VICTORASSO JARDIM, B - 114 THE SELECTION OF TUBULOGENESIS-DEFECTIVE/HIGHLY PROLIFERATIVE ENDOTHELIAL CELLS BY TENASCIN-C IN THE EXTRACELLULAR MATRIX OF GLIOMA CELLS INVOLVES THE OPPOSITE MODULATION OF PKCALPHA AND DELTA ISOFORMS. ALINE OLIVEIRA DA SILVA, TERCIA RODRIGUES ALVES, VIVALDO MOURA NETO, VERÔNICA MARIA MORANDI DA SILVA B - 115 SEX HORMONES INFLUENCE ADAMTS PROTEASE LEVELS IN OVARIAN TUMOR CELLS. MAÍRA DE ASSIS LIMA, VANESSA MORAIS FREITAS B - 116 THE EXPRESSION OF STAT3 IN THE NUCLEUS IS ASSOCIATED WITH PROLIFERATION IN GLIOBLASTOMA MULTIFORME. BRUNA ROZ RODRIGUES, MARILENE HOHMUTH LOPES, ISABELA WERNECK DA CUNHA, VILMA REGINA MARTINS B - 117 NF-KB COORDINATES EPITHELIAL-MESENCHYMAL TRANSITION PROPERTIES IN BREAST CANCER CELLS. BRUNO RICARDO BARRETO PIRES, ANDRE LUIZ MENCALHA, AMANDA DE MORAES MAIA, ELIANA SAUL FURQUIM WERNECK ABDELHAY B - 118 MODULATION OF ENDOTHELIAL CELLS BY HUMAN TUMOR MICROENVIRONMENT: A ROLE FOR SYNTHETIC ANALOGUES OF LIPOXINS. ANDREZA MAIA VIEIRA, EDWARD HELAL NETO, CAMILA CASTRO FIGUEIREDO, THEREZA CHRISTINA BARJA-FIDALGO, IOLANDA M. FIERRO, VERÔNICA MARIA MORANDI DA SILVA 93 B - 119 MALIGNANT HODGKIN CELLS RELEASE CD30 ON MICROVESICLES TO FACILITATE CROSSTALK WITH CD30L ON DISTANT IMMUNE CELLS OF THE TUMOR MICROENVIRONMENT, IN VITRO. HINRICH P HANSEN, ELKE POGGE-VONSTRANDMANN, ADRIANA F PAES LEME, HANNA M ENGELS, VIJAYA L SIMHADRI, MARIA DAMS, ROLF SCHUBERT, FABIO QUONDAMATTEO B - 120 CELL PROLIFERATION EVALUATION OF THE CUTANEOUS SQUAMOUS CELL CARCINOMA IN MICE AFTER PHOTODYNAMIC THERAPY. ANA PAULA DA SILVA, FRANCISCO JAVIER HERNANDEZ BLAZQUEZ, DIVINOMAR SEVERINO, MAURICIO DA SILVA BAPTISTA, BRUNO COGLIATI, MARIA LÚCIA ZAIDAN DAGLI, ELISANGELA DOS ANJOS SILVA, CAMILA LIMA NEVES, JOSÉ ROBERTO MACHADO CUNHA DA SILVA B - 121 EVALUATION OF PRENEOPLASTIC LESIONS AND CELL PROLIFERATION IN EXPERIMENTAL HEPATOCARCINOGENESIS DEVELOPED IN CIRRHOTIC MICROENVIRONMENT. TANIA CRISTINA LIMA, CINTIA MARIA MONTEIRO DE ARAUJO, VENANCIO AVANCINI FERREIRA ALVES, MARIA LUCIA ZAIDAN DAGLI, BRUNO COGLIATI, FRANCISCO JAVIER HERNANDEZ BLAZQUEZ, JOSE ROBERTO MACHADO CUNHA DA SILVA B - 122 THE ROLE OF FMNL1 IN LEUKEMOGENESIS. PATRICIA MARIA BERGAMO FAVARO, JOAO AGOSTINHO MACHADO NETO, MARIANA LAZARINI, FABIOLA TRAINA, MATHEUS RODRIGUES LOPES, ELVIRA INFANTE, ANNE RIDLEY, FERNANDO FERREIRA COSTA, SARA OLALLA-SAAD B - 123 QUERCETIN: POSSIBLE EFFECT ANTI-MDR IN ERYTHROLEUKEMIA HUMAN CELL LINES. MAIARA BERNARDES MARQUES, REGINA COIMBRA ROLA, ANA PAULA DE SOUZA VOTTO GONÇALVES, JULIANO DOMIRACI PACCEZ, LUIZ ZERBINI, SILVYA STUCHI MARIA ENGLER, ANDRÉIA BUFFON, MÁRCIA ROSÂNGELA WINK B - 136 ENTEROLOBIUM CONTORTISILIQUUM TRYPSIN INHIBITOR (ECTI), A PLANT PROTEINASE INHIBITOR, DECREASES IN VITRO CELL ADHESION AND INVASION BY INHIBITION OF SRC-FAK SIGNALING PATHWAYS. CLÁUDIA ALESSANDRA ANDRADE DE PAULA, VIVIEN JANE COULSON-THOMAS, JOANA GASPERAZZO FERREIRA, PALOMA KOREHISA MAZA, ERIKA SUZUKI, ADRIANA MITI NAKAHATA, HELENA BONCIANI NADER, MISAKO UEMURA SAMPAIO, MARIA LUIZA VILELA OLIVA B - 137 EXPRESSION OF HPSE1 BY PROSTATE CELL LINES AND ITS CONTRIBUTION TO TUMOR MICROENVIRONMENT. TAIZE MACHADO AUGUSTO, ANA MILENA HERRERA, HERNANDES F CARVALHO B - 138 XENOGRAPHIC TUMOR GROWTH USING LNCAP PROSTATE CANCER CELL LINE IN IMMUNE COMPETENT MICE. ANA MILENA HERRERA TORRES, TAIZE MACHADO AUGUSTO, HERNANDES F CARVALHO B - 139 FUCAN B FROM BROWN SEAWEED SPATOGLOSSUM SCHRÖEDERI AFFECTS VIABILITY OF DIFFERENT CANCER CELL LINEAGES AND INHIBITS ANGIOGENESIS. LEONARDO THIAGO DUARTE BARRETO NOBRE, ARTHUR ANTHUNES JÁCOME VIDAL, JAILMA ALMEIDA LIMA, RENAN PELLUZZI CAVALHEIRO, EDUARDO HENRIQUE CUNHA DE FARIAS, EDGAR JULIAN PAREDES GAMERO, HELENA BONCIANI NADER, HUGO ALEXANDRE DE OLIVEIRA ROCHA B - 140 SURVIVIN AND XIAP EXPRESSION MODULATION IS ASSOCIATED TO DOCETAXEL-INDUCED CELL DEATH IN BREAST CANCER CELLS. DEBORAH DELBUE DA SILVA, GABRIELA NESTAL DE MORAES, RAQUEL CIUVALSCHI MAIA B - 124 ANTIPROLIFERATIVE ACTIVITY OF LIPIDIC EXTRACTS OF MARINE MICROALGAE ON A MELANOMA CELL LINE: PARTICIPATION OF OMEGA-3 PUFA? RENATA OTTES VASCONCELOS, MICHELE MORAES DE SOUZA, ELIANA BADIALE FURLONG, PAULO CESAR OLIVEIRA VERGNE DE ABREU, MILENE MEDEIROS DE MORAES, JULIANA RAMOS GONZALEZ, ANA PAULA DE SOUZA VOTTO, GILMA SANTOS TRINDADE B - 141 ACTION OF HEAT SHOCK PROTEINS IN THERAPY PHOTODYNAMIC CELLS IN PROSTATE CANCER. ERIANE ELLER DE SIQUEIRA, JULIANA FREIRES MANGOLIN, ANDREZA CRISTINA DE SIQUEIRA SILVA, NEWTON SOARES DA SILVA, CRISTINA PACHECO SOARES B - 125 LAMININ-DERIVED PEPTIDE C16 INDUCES INVASION AND INVADOPODIA FORMATION IN ORAL SQUAMOUS CELL CARCINOMA AND FIBROSARCOMA CELLS. ADRIANE SOUSA DE SIQUEIRA, MONIQUE PEREIRA PINTO, MÁRIO COSTA CRUZ, VANESSA MORAIS FREITAS, RUY GASTALDONI JAEGER B - 142 GENOTOXICITY ASSESSMENT OF PHOTODYNAMIC THERAPY WITH CHLORO-ALUMINUM PHTHALOCYANINE AND CHLORO-ALUMINUM PHTHALOCYANINE IN DIFFERENT CELL LINES. ANDREZA CRISTINA DE SIQUEIRA SILVA, ERIANE ELLER DE SIQUEIRA, JULIANA FREIRES MANGOLIN, NEWTON SOARES DA SILVA, ANTONIO CLÁUDIO TEDESCO, ANDREZA RIBEIRO SIMIONI, CRISTINA PACHECO SOARES B - 126 INHIBITION OF HEDGEHOG PATHWAY IN LEUKEMIC CELL LINEAGE RESULTS IN CELL CYCLE ARREST, DECREASE IN PROLIFERATION AND CLONOGENIC ABILITY WITHOUT INDUCED APOPTOSIS. JULIANA M XAVIER, SARA TEREZINHA OLALLA SAAD B -127 TUMOR METABOLITES MODULATE THE INTERACTION OF ENDOTHELIAL PROGENITOR CELLS AND MATURE ENDOTHELIAL CELLS: ROLE OF MATRIX METALLOPROTEASES AND AKT SIGNALING PATHWAY. FERNANDA RODRIGUES LANZANA FERREIRA, VERÔNICA MORANDI, CAMILA CASTRO FIGUEIREDO B - 128 EFFECTS OF FLAVONOIDS ON GLIOBLASTOMA CELLS. JULIANA MOREIRA SOARES, ANTONIO GOMES SOARES, VIVALDO MOURA NETO, LUCIANA FERREIRA ROMÃO B - 129 PARTIAL CHARACTERIZATION AND EVALUATION OF ANTITUMOR POTENTIAL OF AN L-AMINO ACID OXIDASE OBTAINED OF BOTHROPS JARARACUSSU. NATALIA CRISTINA SANTOS COSTA, MONISE VIANA ABRANCHES, LORENA NACIF MARÇAL, GRACIELLE RODRIGUES PEREIRA, HELIOMAR CAZELLI DE OLIVEIRA FILHO, RENATO NEVES FEIO, SÉRGIO OLIVEIRA DE PAULA, LEANDRO LICURSI DE OLIVEIRA B - 130 VINCRISTINE AND CHRYSOTILE INDUCE MULTIPOLAR MITOSIS IN LUNG CANCER CELLS BY DIFFERENT MECHANISMS. BEATRIZ DE ARAUJO CORTEZ, LUANA RIBEIRO RICARDI, GLAUCIA MARIA MACHADO SANTELLI B - 131 INHIBITION OF THE V-TYPE H+-ATPASE AFFECTS THE APOPTOSIS, MIGRATION AND INVASION CAPACITIES OF MELANOMA CELL LINES. GILDEÍDE APARECIDA COSTA, BRUNNA XAVIER MARTINS, DANIELE SEIPEL DA SILVA, ANDREA VETÖ ARNHOLDT, ANNA LVOVNA OKOROKOVA-FAÇANHA, ARNOLDO ROCHA FAÇANHA. B - 132 CHARACTERIZATION OF THE EXPRESSION OF NA+, K+-ATPASE SUBUNITS IN HUMAN BREAST CANCER CELLS. MELINA ALMEIDA DIAS, MÁRCIA ALVES MARQUES CAPELLA, ANÍBAL GIL LOPES B - 133 EFFECT OF S-NITROSOGLUTATHIONE ON 5-FLUOROURACIL INDUCED EXPERIMENTAL ORAL MUCOSITIS AND THE IMPACT OF ORAL MUCOSITIS ON BACTERIAL FLORA OF HAMSTERS. MARIA ADRIANA SKEFF DE PAULA MIRANDA, ANA PAULA VIEIRA COLOMBO, CARINA MACIEL DA SILVABOGHOSSIAN, CÍNTIA DE MELO BRAGA, MATHEUS MARTINS CAVALCANTE, VIVALDO MOURA NETO, RENATA FERREIRA DE CARVALHO LEITÃO B - 134 CELL MIGRATION IN T-CELL LYMPHOBLASTIC LEUKEMIA/LYMPHOMA: THE ROLE OF THE SPHINGOSINE-1-PHOSFATE RECEPTOR 1. CAROLINA VALENÇA MESSIAS RACHID, JULIA PEREIRA LEMOS, WILSON SAVINO, DANIELLA ARÊAS MENDES-DA-CRUZ B - 135 THE HUMAN METASTATIC MELANOMA CELL LINE, SKMEL147, PRESENTS A DIFFERENT NUCLEOTIDE DEGRADATION PROFILE IN COMPARISON TO MELANOCYTES. CAROLINE GUERRA MARANGON, JÉSSICA MIETHICKI DA SILVA B - 143 STUDY OF CANCER STEM CELLS IN INFLAMMATION ASSOCIATED COLORECTAL CANCER USING THE AOM-DSS MODEL. DYANNA GALAXE DE MATOS, CLAUDIO BERNARDAZZI, LUCAS LOBIANCO DE MATHEO, ANA CAROLINA DUDENHOEFFER CARNEIRO, HEITOR SIFFERT PEREIRA DE SOUZA, ROSSANA COLLA SOLETTI, HELENA LOBO BORGES B - 144 QUANTITATIVE PROTEOME EXPRESSION ANALYSIS OF HUMAN BREAST CANCER CELL LINES T47D AND MCF-7. DENISE DE ABREU PEREIRA, VANESSA SANDIM SIQUEIRA, ANNELIESE FORTUNA DE AZEVEDO FREIRE DA COSTA, ARACI DA ROCHA RONDON, ANA LÚCIA DE OLIVEIRA CARVALHO, MARIA ISABEL DORIA ROSSI, DÁRIO ELUAN KALUME, RUSSOLINA BENEDETA ZINGALI B - 145 EXTRACT PLANT FROM D.PICTA: A POTENTIAL ANTIPROLIFERATIVE AGAINST TUMOR CELLS. CECÍLIO PURCINO DA SILVA SOUZA NETO, ANA CLARA QUEIROZ B - 146 ANTI-TUMORAL AND ANTI-ANGIOGENIC EFFECTS OF FUCSULF-I, A SULFATED FUCAN FROM LYTECHINUS VARIEGATUS. VIVIANE WALLERSTEIN MIGNONE DANTAS, ELIENE KOZLOWSKI, MAURO PAVÃO, PAULO MOURÃO, CAMILA CASTRO FIGUEIREDO, VERÔNICA MORANDI B - 147 EVALUATION OF THE CELL VIABILITY FROM FEMALE CANCER CELL LINES AFTER TREATMENT WITH DIFFERENT FRACTIONS OF B. ARTICULATA. GABRIEL FERNANDES SILVEIRA, SCHERON RATHKE GIUBEL, KETLEN DA SILVEIRA MORAES, CRISTIANE BERNARDES DE OLIVEIRA, GRACE GOSMANN, ANDRÉIA BUFFON, ALESSANDRA NEJAR BRUNO B - 148 PATTERNS OF PROTEOGLYCANS EXPRESSION IN CACO-2 AND HCT116 COLORECTAL CANCER CELLS INDUCED BY VIOLACEIN. GRACE RICHTER MOYSÉS, CAROLINA MELONI VICENTE, LENY TOMA, HELENA BONCIANI NADER, GISELLE ZENKER JUSTO B - 149 THE ROLE OF CELLULAR REDOX POTENTIAL AND THE EFFECTIVENESS OF CHEMOTHERAPY IN NON SMALL CELL LUNG CANCER. VALESKA AGUIAR DE OLIVEIRA, LEONARDO LISBÔA DA MOTTA, FERNANDA MARTINS LOPES, MARCO ANTÔNIO DE BASTIANI, FABIO KLAMT B - 150 HIGH TUMORIGENIC CELLS IN HEAD AND NECK CANCER. ANA LUÍSA HOMEM DE CARVALHO, LAURA DE CAMPOS HILDEBRAND, ISABEL DA SILVA LAUXEN, CARLOS THADEU CERSKI, JACQUES EDUARDO NÖR, MANOEL SANT”ANA FILHO B - 151 IMMUNOLOCALIZATION OF TIMP-2 DURING TUMOR PROGRESSION AZOXYMETHANE INDUCED IN THE LARGE INTESTINE OF ADULT RATS. JAIME RIBEIRO FREITAS, ELIAKIN ROBERTO DO CARMO, JESSICA DA SILVA, KAMILA CAROLINE CAMARGO, LAÍS COSTA AYUB, PEDRO DUARTE NOVAES, CARLA CRISTINE KANUNFRE, MARIA ALBERTINA DE MIRANDA SOARES, JOSÉ ROSA GOMES 94 B - 152 INFLUENCE OF NUCLEOTIDE EXCISION REPAIR IN MITOXANTRONE CITOTOXICITY. FRANCIELE FACCIO BUSATTO, JAQUELINE CESAR ROCHA, JENIFER SAFFI B - 153 DIFFERENTIAL EXPRESSION OF NTPDASES AND ECTO-5’NUCLEOTIDASE IN CERVICAL CARCINOMA CELLS. ALINE BECKENKAMP, DANIELLE BERTODO SANTANA, CAROLINE GUERRA MARANGON, ALESSANDRA NEJAR BRUNO, EMERSON ANDRÉ CASALI, LUCIANE NOAL CALIL, LUIZ FERNANDO ZERBINI, JULIANO PACCEZ, GUIDO LENZ, MÁRCIA ROSÂNGELA WINK, ANDRÉIA BUFFON B - 154 ABCB1 EFFECT ON ARSENIC TRIOXIDE RESISTANCE IN CHRONIC MYELOID LEUKEMIA CELL LINES. RAPHAEL SILVEIRA VIDAL, NATHALIE HENRIQUES SILVA CANEDO, VIVIAN MARY BARRAL DODD RUMJANEK, MARIA DA GLORIA DA COSTA CARVALHO B - 155 EVALUATION OF NMDA RECEPTOR EXPRESSION AND EFFECT OF THE NMDAR ANTAGONISTS ON THE CELL VIABILITY OF CENTRAL NERVOUS SYSTEM TUMORS. DÉBORA SCHOENFELD PRUSCH, CAROLINE BRUNETTO DE FARIAS, GILBERTO SCHWARTSMANN, ANA LUCIA ABUJAMRA, RAFAEL ROESLER B - 156 AUTOPHAGY ACTIVATION IN COLORECTAL CANCER CONTRIBUTE TO THE TOLERANCE OF OXALIPLATIN UNDER ENERGY STRESS CONDITIONS. DIANA LILIAN BORDIN, MICHELLE DE SOUZA LIMA LIMA, GUIDO LENZ, JOÃO ANTONIO PEGAS HENRIQUES B - 157 EFFECTS OF AN RGD-DISINTEGRIN IN BREAST CANCER. CARMEN LUCIA SALLA PONTES, RADU O MINEA, STEVE SWENSON, FRANCIS MARKLAND JR, HELOISA SOBREIRO SELISTRE DE ARAÚJO B - 158 METASTATIC MELANOMA: NEW TARGETS FOR DIAGNOSIS AND TREATMENT. RAUL FERRAZ ARRUDA, WILLIAM RODRIGUES FREITAS, MILTON MASAHIKO KANASHIRO, NADIR FRANCISCA SANNT'ANNA, ARNOLDO ROCHA FAÇANHA B - 159 EXPRESSION AND FUNCTION OF THE CLOTTING INITIATOR PROTEIN, TISSUE FACTOR, CORRELATE WITH CANCER STEM CELLS PHENOTYPE IN HUMAN BREAST CANCER CELL LINES. ARACI MARIA DA ROCHA RONDON, ANNELIESE FORTUNA DE AZEVEDO FREIRE DA COSTA, ANA PAULA DANTAS NUNES DE BARROS, LUIZE GONÇALVES LIMA, ROBSON DE QUEIROZ MONTEIRO, MARIA ISABEL DORIA ROSSI B -160 CHEMICAL STUDIES AND EVALUATION OF BACCHARIS TRIMERA IN CERVICAL CARCINOMA CELL LINE. OLIVEIRA CB, MACIEL ES, GIUBEL SR, MESQUITA CB, COMUNELLO LN, SILVEIRA GF, BRUNO AN, BUFFON A, GOSMANN G B - 161 STUDY OF REARRANGEMENTS BCR-ABL P190 AND P210 IN ADULT ACUTE LYMPHOBLASTIC LEUKEMIA. RUI MILTON PATRÍCIO DA SILVA JÚNIOR, JULIANA FERNANDA HOLANDA BEZERRA, AUDREY VIOLETA MARTINS DE VASCONCELOS, TÂNIA MARIA ROCHA GUIMARÃES, WASHINGTON BATISTA NEVES, FÁRIDA COELI DE BARROS CORREIA MELO, RAUL ANTÔNIO MORAIS MELO B - 162 EPITHELIAL OVARIAN CANCER CELL LINE CLASSIFICATION MODEL BASED ON MOLECULAR HETEROGENEITY AND CHEMOTHERAPY RESPONSE. GUILHERME B FORTES, HAYNNA P KIMIE INADA, JOYCE L MORAES, CINTHYA STERNBERG B - 163 EVALUATION OF CELL PROLIFERATION IN CANINE MALIGNANT MAMMARY TUMORS. CRISTINA MENDES PLIEGO, FRANCIELE BASSO FERNANDES SILVA, JULIANA DA SILVA LEITE, MARCELA FREIRE VALLIM DE MELLO, GABRIELLA CARVALHO MATTOS FERREIRA, MARIA DE LOURDES GONÇALVES FERREIRA, ANA MARIA REIS FERREIRA B - 164 EVALUATION OF PROGRAMMED CELL DEATH IN MAMMARY CARCINOMAS OF FEMALE DOGS. FRANCIELE BASSO FERNANDES SILVA, CRISTINA MENDES PLIEGO, JULIANA DA SILVA LEITE, MARCELA FREIRE VALLIM DE MELLO, BETTINA CAMPOS BRITO CUNHA, MARIA DE LOURDES GONÇALVES FERREIRA, ANA MARIA REIS FERREIRA B - 165 MECHANISMS OF ACETYLEUGENOL NANOCAPSULES ON TOXICITY OF MELANOMA CELLS. CARINE CRISTIANE DREWES, LUANA ALMEIDA FIEL, ADRIANA R. POHLMANN, SÍLVIA S. GUTERRES, SANDRA HELENA P. FARSKY B - 166 P53-DEFICIENT MELANOMA CELLS ARE MORE SENSITIVE TO THE CYTOTOXIC EFFECTS OF UVB IRRADIATION: INVOLVEMENT OF DNA REPAIR ACTIVATION PATHWAYS AND PEROXIDE PRODUCTION. GUILHERME FRANCISCO, TAYNAH IBRAHIM PICOLO DAVID, BRYAN ERIC STRAUSS, ROGER CHAMMAS B - 167 LAMININ-DERIVED PEPTIDES AG73 AND C16 REGULATE MIGRATION AND INVASION OF A HUMAN PROSTATIC CARCINOMA CELL LINE. ADRIANE SOUSA DE SIQUEIRA, TAÍZE M. AUGUSTO, HERNANDES F. CARVALHO, RUY GASTALDONI JAEGER B - 168 INFLUENCE OF THE ADAMTS-1 BREAST TUMOR MICROENVIRONMENT. THAIOMARA ALVES SILVA, ADRIANE S. SIQUEIRA, MÁRIO C. CRUZ, RUY G. JAEGER, VANESSA M. FREITAS B - 169 VASCULAR ENDOTHELIAL GROWTH FACTOR 936C/T POLYMORPHISM IN BRAZILIAN PATIENTS WITH ORAL SQUAMOUS CELL CARCINOMA (OSCC). JÚLIA SALLABERRY PINTO, FERNANDA NEDEL, TIAGO VEIRAS COLLARES, FABIANA KÖMMLING SEIXAS, SANDRA BEATRIZ CHAVES TARQUINIO B - 170 ECTO-ADENOSINE DEAMINASE CHARACTERIZATION ACTIVITY IN HUMAN CERVICAL CARCINOMA CELLS. DANIELLE BERTODO SANTANA, ALINE BECKENKAMP, JÉSSICA NASCIMENTO, ALESSANDRA NEJAR BRUNO, ANDRÉIA BUFFON B - 171 ATL-1, A SYNTHETIC ANALOG OF 15-EPI-LIPOXIN A4, MODULATES KEY FUNCTION OF TUMOR-ASSOCIATED MACROPHAGE: A POTENTIAL ANTITUMORAL TOOL. NATÁLIA MESQUITA DE BRITO, RAFAEL LOUREIRO SIMÕES, IOLANDA MARGHERITA FIERRO, THEREZA CHRISTINA BARJA FIDALGO B - 172 ENHANCED ANTITUMOR EFFECTS OF 3,4-DIHYDROPYRIMIDINE DERIVATIVES (DHPMS) ON HUMAN BREAST CANCER CELLS. BRUNA CÂNDIDO GUIDO, LUCIANA M. RAMOS, CATHARINE C. NÓBREGA, BRENNO A. D. NETO, JOSÉ R. CORRÊA B - 173 CELLULAR RESPONSE ANALYSIS IN COLORECTAL CANCER AND LUNG CANCER CELLS EXPOSED TO TREATMENT WITH PLATINUM AGENTS IN LOW GLUCOSE CONDITIONS. MICHELLE DE SOUZA LIMA, DIANA LILIAN BORDIN, GUIDO LENZ, JOÃO ANTÔNIO PEGAS HENRIQUES B - 174 ANXA1 SUBCELLULAR LOCALIZATION AND CELL PROLIFERATION. LARA VECCHI, LARISSA PRADO MAIA, BRUNA FRANÇA MATIAS, LUIZ RICARDO GOULART FILHO B - 175 IMMUNOHISTOCHEMICAL STUDY OF FIBROBLAST GROWTH FACTOR-2 (FGF-2) IN MALIGNANT AND BENIGN SALIVARY GLAND TUMORS. DÉBORA OLIVEIRA SANTOS, TAMIRIS SABRINA RODRIGUES, SERGIO VITORINO CARDOSO, KAREN RENATA NAKAMURA HIRAKI B - 176 SECRETOME: A RESERVOIR OF BIOACTIVE MOLECULES. REBECA KAWAHARA, ANNELIZE Z.B. ARAGÃO, RAFAEL R. CANEVAROLO, GABRIELA V. MEIRELLES, FERNANDO M. SIMABUCO, RONEI J. POPPI, ISADORA L. FLORES, RICARDO D. COLETTA, NICHOLAS E. SHERMAN, ADRIANA F. PAES LEME B - 177 ANALYSIS OF THE EFFECT OF CYCLOOXYGENASE ON THE EXPRESSION AND ACTIVITY OF MULTIPLE DRUG RESISTANCE PROTEINS (MDRP) IN HUMAN GLIOMA. FERNANDA DE OLIVEIRA SERACHI, ALISON COLQUHOUN B - 178 ANALYSIS OF THE EFFECT OF PROSTAGLANDIN E2 AND IBUPROFEN ON CELL NUMBER AND EXTRACELLULAR MATRIX SYNTHESIS IN U87MG AND U251MG HUMAN GLIOMA CELL LINES. FABIO FEITOZA, ALISON COLQUHOUN B - 179 FUNCTIONAL CHARACTERIZATION OF THE LEUKEMIC STEM CELL IN THE ACUTE PROMIELOCYTIC LEUKEMIA. LUCIANA MARIA FONTANARI KRAUSE, ALEXANDRE KRAUSE, HELDER HENRIQUE PAIVA, EDUARDO MAGALHÃES REGO B - 180 EVALUATION OF THE CYTOTOXIC ACTIVITY OF NEW NITROFURANTOIN DERIVATIVES. JEYCE KELLE FERREIRA DE ANDRADE, MARIA DO DESTERRO RODRIGUES, LARISSA CARDOSO CORRÊA DE ARAÚJO, PAULO BRUNO NORBERTO DA SILVA, DALCI JOSÉ BRONDANI, MANOEL ADRIÃO GOMES FILHO, GARDENIA CARMEN GADELHA MILITÃO, TERESINHA GOLÇALVES DA SILVA B - 181 THE ROLE OF CD73 IN THE PROGNOSIS OF HUMAN MEDULOBLASTOMA CELL LINES. CAPPELLARI, A.R., DIETRICH, F., ROCKEMBACH, L., CLARIMUNDO, V., BRAGANHOL, E., ABUJAMRA , A.L., ROESLER, R., HENNING, U., BATTATASTINI, A.M.O. B - 182 ACTION OF PHOTODYNAMIC THERAPY IN THE EXPRESSION OF ADHESION PROTEINS. CAROLINA GENÚNCIO DA CUNHA MENEZES COSTA, KAREN CRISTIANE MARTINEZ DE MORAES, NEWTON SOARES DA SILVA, CRISTINA PACHECO SOARES B - 183 ANALISYS GENETIC AND MICROSCOPIC OF FIBROPAPILLOMATOSIS IN CHELONIA MYDAS. SAMARA MAFTOUM COSTA, CAROLINA GENÚNCIO DA CUNHA MENEZES COSTA, CRISTINA PACHECO SOARES B - 184 ROLE OF DERMCIDIN IN TOMORIGENESIS OF MELANOMAS. BEATRIZ SANGIULIANO, MARCELA PEREZ, ALINE CADURIN, ANDREW AGUIAR, JOSÉ BELIZÁRIO B - 185 MODULATION OF ENDOTHELIAL CELL ADHESION AND TUBULOGENESIS IN A MODEL OF GLIOMA CELLS SILENCED FOR TENASCIN-C (TNC) EXPRESSION. LAILA RIBEIRO FERNANDES, ADELAIDE CRISTINA DA SILVA MONTEIRO, KELLI CRISTINA MICOCCI, ALINE OLIVEIRA DA SILVA, TERCIA RODRIGUES ALVES, HELOISA SOBREIRO SELISTRE DE ARAUJO, VIVALDO MOURA NETO, VERÔNICA MARIA MORANDI DA SILVA B - 186 ALKALINE PHOSPHATASE EXPRESSION IN INTESTINAL EPITHELIUM OF RATS INJECTED WITH AZOXYMETHANE. LAÍS COSTA AYUB, JAIME RIBEIRO FREITAS, KAMILA CAROLINE CAMARGO, PEDRO DUARTE NOVAES, MARIA ALBERTINA DE MIRANDA SOARES, CRISTINA LÚCIA SANT”ANA COSTA AYUB, NÁDIA FAYEZ OMAR, JOSÉ ROSA GOMES B - 187 CYTOTOXIC EFFECTS ON ORAL SQUAMOUS CELL CARCINOMA (OSCC) INDUCED BY EXTRACTS FROM THE ARISTOLOCHIA GENUS. NAYARA SANTOS DE MATOS, ANDREA BARRETTO MOTOYAMA B - 188 EVALUATION OF THE ROLE OF CISPLATIN AS SENSITIZER TO TRAIL IN BREAST CANCER CELL LINES. VIVIANE ALVES MONTEIRO, CARLOS GIL FERREIRA, CINTHYA STERNBERG B - 189 INVESTIGATION OF ANTITUMOR ACTIVITY OF 1,3-THIAZIN-2,4DIONES AGAINST MELANOMA CELLS. LAURA SARTORI ASSUNÇÃO, MISAEL 95 FERREIRA, FABIOLA FILIPPIN MONTEIRO, MARCUS MANDOLESI SÁ, TANIA BEATRIZ CRECZYNSKI-PASA B - 190 VIOLACEIN INDUCES SPECIFIC ALTERATIONS IN PROTEOGLYCANS PROFILE IN CHRONIC MYELOID LEUKEMIAS. MARCELLY VALLE PALLADINO, MARIA APARECIDA DA SILVA PINHAL, JULIANA LUPORINI DREYFUSS, ELSA YOKO KOBAYASHI, HELENA BONCIANI NADER, GISELLE ZENKER JUSTO B - 191 DUAL ROLE OF TGFBETA DURING COLORECTAL CANCER PROGRESSION. PEDRO HENRIQUE SCHUMANN LIMA, MARCELO NEVES TANAKA, BRUNO K. ROBBS, JOÃO PAULO DE BIASO VIOLA, JOSÉ ANDRÉS MORGADO-DÍAZ B -192 INHIBITION OF MELANOMA CELL MIGRATION BY CINNAMIC ACID: AN IN VITRO STUDY ANALI DEL MILAGROS BERNABE GARNIQUE, GLÁUCIA MARIA MACHADO-SANTELLI, EVANDRO LUÍS DE OLIVEIRA NIERO B - 193 SPARC/OSTEONECTIN EXPRESSION MEDIATES CHEMOSENSITIVITY TO DOCETAXEL IN MCF-7 BREAST CANCER CELLS. ÚRSULA URIAS, MARIA APARECIDA NAGAI B - 194 CHARACTERIZATION OF TUMOR STEM CELLS IN HUMAN GLIOBLASTOMA. ROSENILDE CARVALHO DE HOLANDA AFONSO, SUZANA ASSAD KAHN, DENISE DA SILVEIRA LOBO, DIANA MATIAS, JANE CRISTINA DE OLIVEIRA FARIA, LUCIANA FERREIRA ROMÃO, CELINA GARCIA DA FONSECA, GRASIELLA MARIA VENTURA MATIOSZEK, ALINE MARIE FERNANDES, STEVENS REHEN, JORGE MARCONDES DE SOUZA, VIVALDO MOURA NETO B - 195 ASSOCIATION OF THYMIDYLATE SYNTHASE AND METHYLENETETRAHYDROFOLATE REDUCTASE POLYMORPHISMS IN PATIENTS WITH ASTROCYTIC TUMORS IN A POPULATION OF NORTHERN BRAZIL. MARIANA DINIZ ARAÚJO, WALLAX AUGUSTO SILVA FERREIRA, SYMARA RODRIGUES-ANTUNES, MARICELE BAIA DOS SANTOS, JOSÉ REGINALDO NASCIMENTO BRITO, DOUGLAS VASCONCELOS, NILSON PRAIA ANSELMO, ROMMEL MARIO RODRIGUEZ BURBANO, MARIA LÚCIA HARADA, BÁRBARA DO NASCIMENTO BORGES B - 196 EXPRESSION OF NEUROMEDIN B AND ITS AGONIST IN HUMAN MEDULLOBLASTOMA. MARIANE DA CUNHA JAEGER, CAROLINA NOR, CAROLINE BRUNETTO DE FARIAS, ANA LUCIA ABUJAMRA, GILBERTO SCHWARTSMANN, ALGEMIR LUNARDI BRUNETTO, RAFAEL ROESLER B - 197 COULD NARINGIN PROTECT THE LIVER OF WISTAR RATS INOCULATED WITH WALKER 256 CARCINOSARCOMA? MARIA APARECIDA DA SILVA DIAMANTE, CAMILA DE ANDRADE CAMARGO, FABRICIA DE SOUZA PREDES, HIROSHI AOYAMA, MARY ANNE HEIDI DOLDER B - 198 THE ROLE OF MTOR IN THE CISPLATIN RESISTANT PHENOTYPE IN OVARIAN CANCER LINEAGE. TACIANE LADISLAU, DÉBORA SILVA, KLESIA PIROLA MADEIRA, RENATA DALMASCHIO DALTOÉ, ALICE LASCHUK HERLINGER, IAN VICTOR SILVA, LETICIA BATISTA AZEVEDO RANGEL B - 199 CLINICOPATHOLOGICAL SIGNIFICANCE OF ADAMTS-1 AND VERSICAN IN OVARY CANCER. JOSÉ ANTONIO ORELLANA TURRI, SUELI NONOGAKI, MARCILEI BUIM, JOEMA FELIPE LIMA, CYNTHIA APARECIDA BUENO DE TOLEDO OSÓRIO, FERNADO AUGUSTO SOARES, VANESSA MORAIS FREITAS B - 200 MUTATIONAL STATUS OF THE TP53 GENE IN CANINE MAMMARY TUMORS. THAMIRYS ALINE SILVA FARO, WALLAX AUGUSTO SILVA FERREIRA, SUELLEN DA GAMA BARBOSA MONGER, LUCIEN ROBERTA VALENTE MIRANDA DE AGUIRRA, WASHINGTON LUIZ ASSUNÇÃO PEREIRA, MARIA LÚCIA HARADA, BÁRBARA DO NASCIMENTO BORGES B - 201 IMPACT OF TUMOR-DERIVED EXTRACELLULAR MATRIX ON ENDOTHELIAL CELL FUNCTIONS AND ITS ASSOCIATION WITH TUMORASSOCIATED ANGIOGENESIS. RENATA MACHADO BRANDÃO COSTA, EDWARD HELAL NETO, ROBERTA FERREIRA GOMES SALDANHA DA GAMA, THEREZA CHRISTINA BARJA-FIDALGO, VERÔNICA MARIA MORANDI DA SILVA B - 202 ANTIPROLIFERATIVE ACTIVITY OF NOVEL 3TRIFLUOR(OXO)PYRIMIDO[1,2-A]BENZIMIDAZOLES COMPOUNDS. CASSIANA MACAGNAN VIAU, NATÁLIA LEGUISAMO, DÉBORA CORREA ESPIÑA, SIMONE SCHNEIDER AMARAL, JENIFER SAFFI B - 203 IN VITRO ANTIPROLIFERATIVE ACTIVITY OF (3Β,6Β,16ΒTRIHYDROXY-LUP-20(29)-ENE) TRITERPENE AGAINST BREAST CANCER CELLS. CASSIANA MACAGNAN VIAU, NATÁLIA LEGUISAMO, DÉBORA CORREA ESPIÑA, JENIFER SAFFI B - 204 PARP INHIBITION EXERTS SYNERGISTIC EFFECT ON THE CARDIOTOXICITY OF TOPOISOMERASE II INHIBITORS. ROBERTO MARQUES DAMIANI, MARIANA LUZZATTO, BRUNA CASTILHOS, DINARA JAQUELINE MOURA, JOÃO ANTONIO PÊGAS HENRIQUES, JENIFER SAFFI B - 205 GENOTOXIC ACTIVITY OF EXTRACTS OF MARINE ALGAE FROM THE COAST OF ALAGOAS – BRAZIL. BRUNNO HENRIQUE DA SILVA, ISA RAFAELLA ROCHA BRITO, ÉLICA AMARA CECÍLIA GUEDES, ANTÔNIO EUZÉBIO GOULART SANT’ANA, RENATO S. RODARTE B - 206 CYTOTOXIC EFFECT OF THE ISATIN DERIVATES ON LEUKEMIC CELL LINES. GLAUCIA VERÍSSIMO FAHEINA MARTINS, CAIO CÉSAR BARBOSA BOMFIM, BRUNA BRAGA DANTAS, CLAUDIO GABRIEL L. JÚNIOR, MÁRIO L.A.A. VASCONCELOS, DEMETRIUS A.M. ARAÚJO B -207 ANALYSIS OF THE LEPTIN SIGNALING PATHWAY COMPONENTS OF THYROID PAPILLARY CARCINOMA OF CHILDREN AND ADOLESCENTS BY IMMUNOSTAINING. ARIO LUCIO CORDEIRO ARAUJO JUNIOR, VIVIANE YOUNESRAPOZO, NAYARA PEIXOTO-SILVA, ELAINE DE OLIVEIRA, PATRÍCIA CRISTINA LISBOA, ROSSANA CORBO, MARCELLI GATTO, ALBANITA VIANA DE OLIVEIRA, EGBERTO GASPAR DE MOURA B - 208 APOPTOSIS INDUCTION BY A NEW DERIVATIVE OF PODOPHYLLOTOXIN IN HL-60 CELLS. GLAUCIA VERÍSSIMO FAHEINA MARTINS, ALETHÉIA LACERDA DA SILVEIRA, BRUNA BRAGA DANTAS, DEMETRIUS ANTONIO MACHADO DE ARAÚJO B -209 A NEW FAB INHIBITS CELL PROLIFERATION IN MCF7 BREAST CARCINOMA CELLS. THAISE GONÇALVES ARAÚJO, CLÁUDIA MENDONÇA RODRIGUES, BRUNA FRANÇA MATIAS, YARA C.PAIVA MAIA, ANGELA A.S. SENA, CAROLINA FERNANDES REIS, CARLOS UEIRA VIEIRA, LUIZ RICARDO GOULART B - 210 INDUCTION OF APOPTOSIS IN HUMAN LUNG ALVEOLAR CARCINOMA EPITHELIAL CELLS (A549) BY THE METALLIC COMPLEX CISTETRAAMMINE(OXALATO)RUTHENIUM(III) DITHIONATE. ELISÂNGELA DE PAULA SILVEIRA-LACERDA, FLÁVIA DE CASTRO PEREIRA, ALINY PEREIRA DE LIMA, WANESSA CARVALHO PIRES, CESAR AUGUSTO SAM TIAGO VILANOVA-COSTA B - 211 BREAST CANCER STEM CELL-LIKE PHENOTYPE OF A SUBPOPULATION DERIVED FROM A LUMINAL CELL LINE. ANNELIESE FORTUNA DE AZEVEDO FREIRE DA COSTA, ANA PAULA DANTAS NUNES DE BARROS, CAMILA MARIA LONGO MACHADO, ARACI MARIA DA ROCHA RONDON, ANDREA CORDOVIL PIRES, HÉLIO DOS SANTOS DUTRA, RADOVAN BOROJEVIC, ELIENE OLIVEIRA KOZLOWSKI, MAURO SÉRGIO GONÇALVES PAVÃO, ROGER CHAMMAS, MARIA ISABEL DORIA ROSSI B - 212 UNDERSTANDING THE ROLE OF GENETIC INSTABILITY IN BASAL CELL CARCINOGENESIS THROUGH ANALYSIS OF POLYMORPHIC REPETITIVE DNA SEQUENCES. JULIANA S. CAPITANIO, MARCOS A. R. MARTINEZ, GUILHERME FRANCISCO, CYRO FESTA-NETO, ITAMAR R. G. RUIZ B - 213 IN VITRO ANTITUMOR ACTIVITY OF TRETINOIN-LOADED LIPIDCORE NANOCAPSULES ON HUMAN LUNG ADENOCARCINOMA EPITHELIAL CELL LINE (A549). EDUARDA SCHULTZE, VIRGINIA YURGEL, KARINE RECH BEGNINI, ALINE FERREIRA OURIQUE, RUY CARLOS RUVER BECK, STANISÇUAKI GUTERRES, ADRIANA RAFFIN POHLMANN, FABIANA SEIXAS, TIAGO COLLARES B - 214 MODULATION OF MELANIN SYNTHESIS BY PLATINUM COMPLEXES (II) WITH HYDANTOIN DERIVATIVE AS A NOVEL ROUTE FOR CYTOTOXICITY IN MELANOMA CELLS. FERNANDA BRANCO FILIPPIN, SUELY LINS GARDINO, MARIA DO CARMO ALVES DE LIMA, IVAN DA ROCHA PITTA, SILVYA STUCHI MARIAENGLER B - 215 B16F10 MELANOMA CELLS GROWN ON COLLAGEN MATRIX EXHIBITED BEHAVIOR SIMILAR TO IN VIVO TUMOR. PAULA MEDEIROS SABINO, BRUNO PIVA, BRUNO LOURENÇO DIAZ B - 216 STRUCTURAL AND ULTRASTRUCTURAL DESCRIPTION OF THE METAPLASIC AND NEOPLASIC PROCESSES IN RAT PROSTATE EPITHELIAL CELLS AFTER SEX STEROIDS-INDUCED CARCINOGENESIS. JAQUELINE DE CARVALHO RINALDI, HELOISA BORTOLIN BRUNO, LIVIA MARIA LACORTE, FLAVIA KARINA DELELLA, LUIS ANTONIO JUSTULIN JUNIOR, SERGIO LUIS FELISBINO B - 217 FREQUENCY OF HPV INFECTION IN HEAD AND NECK SQUAMOUS CELL CARCINOMA AND ITS INFLUENCE IN CELL CYCLE RELATED PROTEINS TOP2A AND MCM2. ANA CAROLINA LAUS, NAIARA CORRÊA NOGUEIRA DE SOUZA, ADHEMAR LONGATTO FILHO, CRISTOVAM SCAPULATEMPO NETO, ANDRÉ LOPES CARVALHO B - 218 KNOCKDOWN OF XIAP COOPERATES WITH THE OVEREXPRESSION OF P53 IN REDUCING CELL PROLIFERATION AND ENHANCING CELL DEATH IN GLIOMAS. ANDREW OLIVEIRA SILVA, MICHELE HÜTTEN, PATRÍCIA LUCIANA DA COSTA LOPEZ, GUIDO LENZ B - 219 THE ROLE OF CELLULAR ADHESION STATE IN REGULATING NUCLEAR AKT/PKB LOCALIZATION IN HUMAN MELANOMA CELL LINES HARBORING DISTINCT ONCOGENC MUTATIONS. SARAH FRANCO FIGUEIRA, RENATA PASCON, MARCELO AFONSO VALLIM, JOEL MACHADO JR. B - 220 MICROARRAY ANALYSIS OF DIFFERENTIALLY EXPRESSED GENES IN ADENOID CYSTIC CARCINOMA CELLS (CAC2) TREATED WITH LAMININ-DERIVED PEPTIDES C16 OR AG73. MICAEL DE PAIVA OLIVEIRA, EMERSON S. SANTOS, VANESSA M. FREITAS, BASILIO SMUCZEK, RUY G. JAEGER B - 221 THE FLAVONOID ISOQUERCITRIN MODULATE PATHWAY WNT/ΒCATENIN THROUGH SPHINGOSINE KINASE IN HUMAN GLIOBLASTOMA CELLS IN VITRO. FERNANDA MIRANDA, DÉBORA MALTA CERQUEIRA, RAFAEL LINDOSO, MARCELO EINICKER-LAMAS, VIVALDO MOURA NETO, JOSÉ GARCIA ABREU B - 222 PRPC AND HOP EXPRESSION AND SECRETION IN COLON AND RECTUM TUMORS ARE ASSOCIATED WITH INVASION. TONIELLI CRISTINA SOUSA DE LACERDA, MARCOS VINICIUS SALLES DIAS, CLEITON FAGUNDES MACHADO, BRUNO COSTA SILVA, VILMA REGINA MARTINS B - 223 EVALUATION OF THE CYTOTOXIC ACTIVITY OF NEW 4THIAZOLIDINONE. MARIA DO DESTERRO RODRIGUES, SANDRINE MARIA ARRUDA DE LIMA, JEYCE KELLE FERREIRA DE ANDRADE, LARISSA CARDOSO CORRÊA DE ARAÚJO, ERALDO ANTUNES GUIMARÃES NETO, JOSÉ GILDO DE LIMA, ALEXANDRE 96 JOSÉ DA SILVA GÓES, TERESINHA GONÇALVESDA SILVA, GARDENIA CARMEN GADELHA MILITAO, SILENE CARNEIRO DO NASCIMENTO AMARO DE MOURA, LUÍS HENRIQUE TOSHIHIRO SAKAMOTO, ANDREA BARRETTO MOTOYAMA, FÁBIO PITTELLA SILVA B - 224 MODULATION OF AUTOPHAGY PATHWAY GENE EXPRESSION AFTER CROTAMINE TREATMENT OF TUMOR CELL LINE. MÁRCIA NEIVA, CAMILA M YONAMINE, MARCELA B NERING, EDUARDO B OLIVEIRA, DANIELE Y SUNAGA, MIRIAN A F HAYASHI B - 241 EFFECT OF KIAA0090 KNOCKDOWN IN SOME ASPECTS OF MELANOMA DEVELOPMENT, MAINTENANCE AND PROGRESSION. RODRIGO RIBEIRO DA SILVA, CARLOS ANTONIO COUTO LIMA, ROBERTO AUGUSTO SILVA MOLINA, NOEMI YASUKO OTSUKA, CIBELE CARDOSO, CRISTIANO GONÇALVES PEREIRA, ENILZA MARIA ESPREAFICO B - 225 AUXOTROPHIC RECOMBINANT BCG OVEREXPRESSING AG85B AS AN ALTERNATIVE THERAPY FOR SUPERFICIAL BLADDER CANCER. KARINE RECH BEGNINI, CAROLINE RIZZI, VINICIUS FARIAS CAMPOS, EDUARDA SCHULTZE, VIRGINIA CAMPELLO YURGEL, TIAGO COLLARES, ODIR DELLAGOSTIN, FABIANA KOMMLING SEIXAS B - 242 EXPRESSION PROFILE OF SUPPRESSOR OF VARIEGATION (SUV) GENE FAMILY IN BREAST CANCER CELL LINES. BRENNO VINÍCIUS MARTINS HENRIQUE, CAROLINA AMARO DE MOURA, ANDREA BARRETO MOTOYAMA, ROSÂNGELA VIEIRA DE ANDRADE, FÁBIO PITTELLA SILVA B - 226 RUTHENIUM COMPLEX COORDENATED WITH LAPACHOL IS CYTOTOXIC FOR DIFFERENT TUMOR CELL LINES AND INHIBITS TUMOR CELL ADHESION. JULIANA UEMA RIBEIRO, MARÍLIA IMACULADA FRAZÃO BARBOSA, MÁRCIA REGINA COMINETTI, ALZIR AZEVEDO BATISTA B -2 43 KIAA0090, A NEW HUMAN GENE IS INVERSELY CORRELATED WITH HER2 EXPRESSION AND ASSOCIATED WITH BREAST CANCER PROGRESSION. ROBERTO AUGUSTO SILVA MOLINA, DANIEL TIEZZI, CIBELE CARDOSO, RODRIGO RIBEIRO DA SILVA, NOEMI Y. OTSUKA, ENILZA MARIA ESPREAFICO B - 227 EVALUATION OF GENOTOXIC EFFECTS IN LYMPHOCYTES OF MICE FROM LEAF EXTRACTS ZIZIPHUS JOAZEIRO MARTIUS (RHAMNACEAE). ISA RAFAELLA ROCHA BRITO, BRUNNO HENRIQUE DA SILVA, CLÁUDIA CAVALCANTE DE MATOS RODARTE, ANTÔNIO EUZÉBIO GOULART SANT’ANA, RENATO S. RODARTE B - 244 CROSSTALK BETWEEN C6 GLIOMA CELLS AND MESENCHYMAL STEM CELLS THROUGH SOLUBLE FACTORS AFFECTS THE PURINERGIC SYSTEM. PAULA ANDREGHETTO BRACCO, SILVIA MULLER MOURA, GIOVANA RAVIZZONI ONZI, LUANA DIMER HAINZENREDER, ADRIANO MARTIMBIANCO DE ASSIS, PEDRO ROOSEVELT TORRES ROMÃO, MÁRCIA ROSÂNGELA WINK B - 228 CONSTRUCTION OF A RECOMBINANT TAT-HA FUSOGENIC MYOSIN-VA FRAGMENT COVERING THE BINDING MOTIF OF DLC2: EVALUATION OF CELL PENETRATING AND PRO-APOPTOTIC PROPERTIES. ENILZA MARIA ESPREAFICO, CLEIDSON DE PÁDUA ALVES, ENILZA MARIA ESPREAFICO B - 245 THE NOVEL CYTOKINE PANDER/FAM3B AFFECTS CELL GROWTH AND INHIBITS APOPTOSIS IN MDA-MB-231 BREAST TUMOR CELLS. LIBNAH LEAL, IZABELA CALDEIRA, HUMBERTO MIGUEL GARAY MALPARTIDA B - 229 EVALUATION OF CITOTOXIC ACTIVITY OF RHIZOPHORA MANGLE ON HELA CELLS. MARLLON ALEX NASCIMENTO SANTANA, ELIANE ALVES BANDEIRA DE CARVALHO, ERWELLY BARROS DE OLIVEIRA, KRÍSIA EMANUELLE FERREIRA DA SILVA, PAULO HENRIQUE CAVALCANTI DE ARAÚJO, ELIETE CAVALCANTI DA SILVA, ANTÔNIO FERNANDO MORAIS DE OLIVEIRA, JEYMESSON RAPHAEL CARDOSO VIEIRA B - 230 THE INFLUENCE OF A SELECTIVE INHIBITOR OF THE TYROSINE PROTEIN KINASE ACTIVITY OF THE TRK FAMILY ON THE VIABILITY OF SH-SY5Y HUMAN NEUROBLASTOMA CELLS. BÁRBARA KUNZLER SOUZA, CAROLINE BRUNETTO DE FARIAS, GILBERTO SCHWARTSMANN, ALGEMIR LUNARDI BRUNETTO, ANA LUCIA ABUJAMRA, RAFAEL ROESLER B - 231 EFFECTS OF A GASTRIN-RELEASING PEPTIDE RECEPTOR ANTAGONIST ON THE VIABILITY OF SH-SY5Y HUMAN NEUROBLASTOMA CELLS. BÁRBARA KUNZLER SOUZA, CAROLINE BRUNETTO DE FARIAS, GILBERTO SCHWARTSMANN, ALGEMIR LUNARDI BRUNETTO, ANA LUCIA ABUJAMRA, RAFAEL ROESLER B - 246 PROFILING OF DIFFERENTIALLY EXPRESSED APOPTOSIS-RELATED GENES IN T47D BREAST CANCER CELLS TREATED WITH ANGIOTENSIN-(1-7). CHERYL ALECRIM SANTOS, SILVANA APARECIDA ALVES CORREA DE NORONHA, SAMUEL RIBEIRO DE NORONHA, SUMA IMURA SHIMUTA, CLOVIS RYUICHI NAKAIE, ISMAEL DALE COTRIM GUERREIRO DA SILVA B - 247 FUNCTIONAL CHARACTERIZATION OF A NOVEL GENE ASSOCIATED WITH MELANOMA PROGRESSION AND BRAF V600E. CRISTIANO G PEREIRA, RODRIGO R SILVA, CIBELE CARDOSO, ENILZA MARIA ESPREAFICO B - 248 INVOLVEMENT OF MYOSIN-VA IN FOCAL ADHESION FORMATION AND SURVIVAL UNDER SUBSTRATE DETACHMENT CONDITIONS. ANELISA RAMÃO, CARMEN LUCIA SALLA PONTES, CLEIDSON PADUA ALVES, ENILZA MARIA ESPREAFICO B - 249 CHARACTERIZATION OF NEW MELANOMA-RESTRICTED GENES ASSOCIATED WITH MAPK PATHWAY. CIBELE CARDOSO, CRISTIANO GONÇALVES PEREIRA, RODRIGO RIBEIRO DA SILVA, ROBERTO AUGUSTO SILVA MOLINA, GUILHERME AUGUSTO SILVA DOS SANTOS, EDUARDO MAGALHÃES REGO, ENILZA MARIA ESPREAFICO B - 232 EFFECT OF NOVEL RATIONALLY DESIGNED NAPHTOQUINONESDERIVED DRUGS ON LUNG CANCER CELL LINES. ALICE LASCHUK HERLINGER, IURI CORDEIRO VALADAO, RENATA DALMASCHIO DALTOÉ, KLESIA PIROLA MADEIRA, JOÃO FRANCISCO ALLOCHIO FILHO, LUCAS CUNHA DIAS DE REZENDE, MURILO FANCHIOTTI CERRI, SARAH FERNANDES TEIXEIRA, PAULO CILAS MORAIS LYRA JUNIOR, SANDRO JOSÉ GRECO, LETICIA BATISTA AZEVEDO RANGEL B - 250 ANTI-FGF2 MONOCLONAL ANTIBODY AS AN IMAGING AND ANTITUMOR APPROACH FOR MURINE MELANOMA B16-F10. RODRIGO BARBOSA DE AGUIAR, CAROLINA BELLINI PARISE, ROGER CHAMMAS, JANE ZVEITER DE MORAES B - 233 CITOTOXIC EFFECTS OF ANTHRACYCLINES IN HUMAN FIBROBLASTS DEFICIENT IN NUCLEOTIDE EXCISION REPAIR. LARISSA MILANO DE SOUZA, TEMENOUGA NIKOLOVA GUESHEVA, GUIDO LENZ, JENIFER SAFFI B - 251 CELL DEATH AND IN VIVO MELANOMA TUMOR GROWTH REMISSION DETERMINED BY A SNAKE TOXIN WITH SPECIFICITY FOR ACTIVELY PROLIFERATING CELLS. MIRIAN A F HAYASHI, FABIO D NASCIMENTO, LUCIE SANCEY, ALEXANDRE PEREIRA, EDUARDO B OLIVEIRA, HELENA B NADER, IVARNE LS TERSARIOL, JEAN-LUC COLL, IRINA KERKIS B - 234 NEW BENZOTHIAZOLE INDUCES GENOTOXICITY IN GASTRIC CANCER CELL LINE BUT NOT IN NORMAL CELLS. BRUNO MOREIRA SOARES, LEILANE DE HOLANDA BARRETO, JORGE AMANDO BATISTA RAMOS, SIVANNE BRAGA DE ALMEIDA, VITOR FRANCISCO FERREIRA, NOGUEIRA, A.F., AZEVEDO, E.C., ROMMEL MARIO RODRIGUÉZ BURBANO, TATIANA VASCONCELOS, MARNE CARVALHO DE VASCONCELLOS, RAQUEL CARVALHO MONTENEGRO B - 235 STUDY THE INVOLVEMENT OF METAL PEPTIDASE PHEX IN SQUAMOUS CELL CARCINOMA. RAQUEL LEÃO NEVES, DANIELA B. ZANATTA, LARISSA P. COPPINI, BRYAN E. STRAUSS, JOÃO B. PESQUERO, FÁBIO D. NASCIMENTO, IVARNE L. TERSARIOL, HELENA B. NADER, ADRIANA K. CARMONA, NILANA M. T. BARROS B - 236 IONIZING RADIATION INDUCES AKT PHOSPHORYLATION AND MIR210 EXPRESSION IN A RADIORESISTANT GLIOBLASTOMA CELL LINE. PAULA SABBO BERNARDO, GISELLE PINTO DE FARIA, RAQUEL CIUVALSCHI MAIA B - 237 UPREGULATION OF APE/REF-1 EXPRESSION INDUCED BY ENDOPLASMIC RETICULUM STRESS. CLARISSA LEAL DE OLIVEIRA MELLO, LUCIANA BARRETO CHIARINI B - 238 EVALUATION OF THE CELLULAR RESPONSE OF SPECIES CEBUS APELLA EXPOSED TO CARCINOGEN N-METHYL-N-NITROSOUREA (MNU) AND TREATED WITH CANOVA®. DANIELLE CRISTINNE AZEVEDO FEIO, JOSÉ AUGUSTO PEREIRA CARNEIRO MUNIZ, ROMMEL MÁRIO RODRIGUEZ BURBANO, LACY CARDOSO DE BRITO JUNIOR, PATRÍCIA DANIELLE LIMA DE LIMA B - 239 LIPID DROPLETS ARE SITES OF THE MTOR PATHWAY IN COLON CANCER CELLS. NARAYANA FAZOLINI BASTOS, JOÃO PAULO DE BIASO VIOLA, PATRÍCIA TORRES BOZZA, CLARISSA MENEZES MAYA-MONTEIRO B - 240 EHMT1 AND EHMT2 METHYLTRANSFERASES EXPRESSION ANALYSIS IN BREAST CANCER CELL LINES. MARTHA SILVA ESTRELA, CAROLINA B - 252 MT1-MMP AS A MOLECULAR MARKER DURING THE FIRST STAGES OF PROGRESSION OF THE COLON CANCER IN RATS. ELIAKIN ROBERTO DO CARMO, ROSIANE CRISTINA ALVES, JAIME RIBEIRO FREITAS, JÉSSICA DA SILVA, KAMILA CAROLINE CAMARGO, PEDRO DUARTE NOVAES, CARLA CRISTINE KANUNFRE, MARIA ALBERTINA DE MIRANDA SOARES, JOSE ROSA GOMES C – Cell Biology and Inflammation C1-C118 C-1 EFFECTS OF ANTI-CD3 MONOCLONAL ANTIBODY IN SALIVARY GLANDS OF SPONTANEOUSLY DIABETIC MICE. EBER EMANUEL MAYORAL, GABRIEL MORETTI DOMINGUES, ALAN TELLES FERRI, RAFAEL DIAS MANCIO, FERNANDA ALVAREZ ROJAS, LUIS ANTONIO PERONI, EDMIR AMERICO LOURENÇO, EDUARDO JOSE CALDEIRA C-2 MELANOMACROPHAGES IN THE SPLEEN OF EUPEMPHIX NATTERERI (ANURA: LEIUPERIDAE): RESPONSES TO LPS. LILIAN FRANCO-BELUSSI, GABRIELA BARONI LEITE, JULIANE SILBERSCHIMDIT FREITAS, CLASSIUS DE OLIVEIRA C-3 FPR RECEPTOR MEDIATES THE ANTI-INFLAMMATORY ACTIONS OF ANNEXIN A1 PROTEIN IN ENDOTOXIN-INDUCED UVEITIS. ANA PAULA GIROL, CRISTIANE DAMAS GIL, SONIA MARIA OLIANI 97 C-4 DNA DAMAGE IN BLOOD OF THE COPD PATIENTS BY COMET ASSAY. HELEN TAIS DA ROSA, ANDRÉA LÚCIA GONÇALVES DA SILVA, MARTIELE BIZARRO, EDUARDA BENDER, PAULO RICARDO DA ROSA, CLARA FORRER CHARLIER, MIRIAM SALVADOR, DINARA JAQUELINE MOURA, ANDRÉIA ROSANE DE MOURA VALIM, NIKOLOVA TEMENOUGA GUECHEVA, JOÃO ANTONIO PEGAS HENRIQUES C-5 RU486 IMPROVES CUTANEOUS WOUND HEALING IN MICE SUBMITTED TO PSYCHOLOGICAL STRESS. TAIS FONTOURA DE ALMEIDA, ANDRÉA MONTE ALTO COSTA C-6 MODULATION OF PROSTATE SMOOTH MUSCLE CELL RESPONSE TO BACTERIAL LPS BY TESTOSTERONE. CAROLINA LEIMGRUBER, AMADO QUINTAR, CRISTINA ALICIA MALDONADO C-7 ANALYSIS OF THE INFLAMMATORY PROFILE IN TYPE 1 AND 2 IN BLOOD AND LESIONS OF MOUSE INFECTED WITH AMERICAN CUTANEOUS LEISHMANIASIS. FLÁVIA PERRIM DE MELO, FÁBIO RIBEIRO QUEIROZ, ANA CRISTINA CARVALHO DE BOTELHO, JOSIANE BARBOSA PIEDADE, LUCIANA MARIA SILVA C-8 INFLUENCE OF CYCLOOXYGENASE-2 INHIBITION ON INTRAMEMBRANOUS AND ENDOCHONDRAL BONE GRAFTS INCORPORATION: IMUNOHISTOCHEMISTRY ANALYSIS. CLÁUDIA CRISTINA BIGUETTI, EDUARDO MORESCHI, LEANDRO DE ANDRADE HOLGADO, APARÍCIO FIUZA DE CARVALHO DEKON, PAULO DOMINGOS RIBEIRO JUNIOR, MARIZA AKEMI MATSUMOTO C - 22 SENSING ENDOPLASMIC RETICULUM STRESS BY PROTEIN KINASE RNA-LIKE ENDOPLASMIC RETICULUM KINASE PROMOTES ADAPTIVE MITOCHONDRIAL DNA BIOGENESIS AND CELL SURVIVAL VIA HEME OXYGENASE1/CARBON MONOXIDE ACTIVITY. HUN TAEG CHUNG, MIN ZHENG, SEUL-KI KIM, YEONSOO JOE, SUNG HOON BACK C - 23 INHIBITION OF ROCK PROMOTES RESOLUTION OF INFLAMMATION BY ENHANCING NEUTROPHIL APOPTOSIS. RAYSSA MACIEL ATHAYDE, ALESANDRA CORTE REIS, VANESSA PINHO DA SILVA, MAURO MARTINS TEIXEIRA C - 24 NANOCOMPOSITE TREATMENT REDUCES THE SEVERITY OF THE INFLAMMATORY RESPONSE ASSOCIATED WITH ACUTE GRAFT VERSUS HOST DISEASE (GVHD) IN MICE. PRISCILA TELES DE TOLÊDO BERNARDES, BÁRBARA MAXIMINO REZENDE, MARINA GOMES MIRANDA E CASTOR ROMERO, DANIELLE SOUZA G, MAURÍCIO VELOSO BRANT PINHEIRO, VANESSA PINHO, MAURO MARTINS TEIXEIRA C - 25 EFFECTS OF HIGH-CARBOHYDRATE DIET IN THE INCREASED EPIDIDYMAL ADIPOSE TISSUE AND THE RECRUITMENT OF INFLAMMATORY CELLS IN MICE. PRISCILA TELES DE TOLÊDO BERNARDES, BÁRBARA MAXIMINO REZENDE, MAURO MARTINS TEIXEIRA, VANESSA PINHO, ADALIENE VERSIANI MATOS C - 26 ANNEXIN A1 PROMOTES RESOLUTION OF ACUTE INFLAMMATION BY INDUCING NEUTROPHIL APOPTOSIS. JULIANA PRISCILA VAGO DA SILVA, CAMILA RODRIGUES CHAVES NOGUEIRA, LUCIANA PÁDUA TAVARES, THAÍS ROLLA DE CAUX, FREDERICO MARIANETTI SORIANI, FERNANDO LOPES, REMO DE CASTRO RUSSO, VANESSA PINHO, MAURO MARTINS TEIXEIRA, LIRLÂNDIA PIRES DE SOUSA C-9 INCREASE OF TRYPTASE MAST CELL LEADS TO DENERVATION IN INFECTED INDIVIDUALS WITH AND WITHOUT CHAGASIC MEGAESOPHAGUS. PATRÍCIA ROCHA MARTINS, RODOLFO DUARTE NASCIMENTO, JACQUELINE GARCIA DUARTE, SHEILA ADAD, DÉBORA D’ÀVILA REIS C - 27 EFFECTS OF THE SYMPATHETIC-PERIPHERAL CRH-HISTAMINE AXIS IN THE IMMUNOLOGICAL FUNCTION OF MACROPHAGES. PATRÍCIA RENCK NUNES, PAULO IVO HOMEM DE BITTENCOURT JÚNIOR C - 10 TRAINING EFFECTS ON THE GOBLET CELLS NUMBER AND INTESTINAL ALKALINE PHOSPHATASE EXPRESSION IN GMS OBESE RATS.JAIME RIBEIRO FREITAS, MARIA ALBERTINA DE MIRANDA SOARES, NAYARA DE CARVALHO LEITE, SABRINA GRASSIOLLI, JOSÉ ROSA GOMES C - 28 DETRIMENTAL ROLE OF INTERLEUKIN-4 IN ACETAMINOPHENINDUCED ACUTE LIVER FAILURE. DANIELE ARAÚJO PIRES, PEDRO ELIAS MARQUES, SYLVIA STELLA MARQUES, JAYANE LAÍS QUINTÃO, LINDSLEY FERREIRA GOMIDES, GUSTAVO BATISTA MENEZES C - 11 THE INFLUENCE OF PROTEIN MALNUTRITION ON THE IL-1Β PRODUCTION BY MACROPHAGES STIMULATED WITH TNF-Α. DALILA CUNHA DE OLIVEIRA, ALEXANDRA SIQUEIRA MELLO, JACKELINE SOARES OLIVEIRA BELTRAN, ED WILSON DOS SANTOS, PRIMAVERA BORELLI, RICARDO AMBRÓSIO FOCK C - 29 EVALUATION OF IMMUNOGENICITY OF THE CANDIDATE PHAGES TO USE IN PHAGE THERAPY AND ANALYSIS OF CROSS-REACTION BY SPECIFIC ANTIBODIES. ROBERTO SOUSA DIAS, VINÍCIUS DUARTE SILVA, FLÁVIA DE OLIVEIRA SOUZA, LÍVIA CARNEIRO FIDÉLIS SILVA, LEANDRO LICURSI DE OLIVEIRA, EDUARDO DE ALMEIDA MARQUES SILVA, CYNTHIA CANEDO SILVA, SÉRGIO OLIVEIRA DE PAULA C - 12 CAFFEIC ACID PHENETHYL ESTER (CAPE) IMPROVES THIRD-DEGREE BURNS HEALING IN RATS. JEANINE SALLES DOS SANTOS, ANDRÉA MONTE-ALTO COSTA C - 13 NEUTROPHIL RECRUITMENT TO THE SKELETAL MUSCLE AFTER EXERCISE TO FATIGUE IS ASSOCIATED TO PRODUCTION OF ROS. ALBENÁ NUNES DA SILVA, PRISCILA TELES DE TOLEDO BERANARDES, BARBARA MAXIMINO REZENDE, FERNANDO LOPES, MAURO MARTINS TEIXEIRA, VANESSA PINHO C - 30 EFFECTS OF THE STIMULATION OF INFLAMMATION AND TREATMENT WITH ANXA1 PROTEIN IN THE HUMAN RETINAL PIGMENT EPITHELIAL CELLS. LAILA TONIOL CARDIN, NATHÁLIA MARTINS SONEHARA, KALLYNE KIOKO MIMURA, LAÍS SOBRAL, ANDRÉIA MACHADO LEOPOLDINO, SONIA MARIA OLIANI, FLÁVIA CRISTINA RODRIGUES LISONI C - 14 LEPTIN EFFECTS ON LEUKOCYTES ON OBESE MICE. GLAUCIA SOUZA ALMEIDA, SALLY LIECHOCKI, LOHANNA PALHINHA DO AMARAL, PATRICIA TORRES BOZZA, CLARISSA M. MAYA-MONTEIRO C - 31 OXIDIZED-LDL AND PARAOXONASE-1 IN THE ASSOCIATION BETWEEN CORONARY ARTERY DISEASE AND SLEEP DISORDERED BREATHING. FERNANDA SCHÄFER HACKENHAAR, DENIS MARTINEZ, TÁSSIA MACHADO MEDEIROS, CRISTINI KLEIN, PAULO V.G. ALABARSE, MARA S. BENFATO C - 15 CXCR2 AND FPR1 ACTIVATION STIMULATE NEUTROPHILMEDIATED INJURY DURING ACETAMINOPHEN-INDUCED ACUTE LIVER FAILURE. PEDRO ELIAS MARQUES, SYLVIA STELLA AMARAL, DANIELE ARAUJO PIRES, LAURA LOPES NOGUEIRA, MAURO MARTINS TEIXEIRA, GUSTAVO BATISTA MENEZES C - 32 OBESITY AS AN ADDITIONAL COMPLICATION FACTOR IN SEPSIS INDUCED PULMONARY INFLAMMATION. THAIS PINEDA FUNGARO, RICARDO COSTA PETRONI, SUELEN JERÔNYMO SOUZA DE OLIVEIRA, DENISE FREDIANE BARBEIRO, FRANCISCO GARCIA SORIANO, THAIS MARTINS DE LIMA-SALGADO C - 16 ESTABLISHMENT OF THE OPTIMAL CONDITIONS TO DIFFERENTIATE THE HUMAN U937 CELLS INTO M1 OR M2 MACROPHAGES TO BE USED AS IN VITRO EXPERIMENTAL MODEL FOR BIOMEDICAL STUDIES. MARCO ANTÔNIO DE BASTIANI, MATHEUS BECKER FREITAS, LEONARDO LISBÔA DA MOTTA, FERNANDA FRANÇA, FÁBIO KLAMT, MELISSA MARKOSKI, CAROLINA BEATRIZ MÜLLER C - 33 INFLAMMATORY CELLS PROFILE IN CARDIAC MUSCLE OF CALOMYS CALLOSUS INFECTED WITH TRYPANOSOMA CRUZI IN THE CHRONIC PHASE OF INFECTION. NOEMI NOSOMI TANIWAKI, VANESSA GALDENO FREITAS, CIBELE RODELLA ALMEIDA, DEBORAH YAMAZAKI HUKUDA, MARIA CRISTINA RODRIGUES MEDEIROS, ANGELA BATISTA GOMES DOS SANTOS C - 17 EXPRESSION PATTERN OF PRO-INFLAMMATORY CYTOKINES IN MSCS FROM GALLUS GALLUS. GABRIHEL STUMPF VIEGAS, RAQUEL CALLONI, PATRICK TÜRCK, DIEGO BONATTO, ELVIRA CORDEIRO C - 34 FEMALE MICE SHOW INCREASED LEUKOCYTE RECRUITMENT IN ADIPOSE TISSUE THAN MALE MICE IN A FOOD ALLERGY MODEL. RAFAELA VAZ SOUSA PEREIRA, NATHÁLIA VIEIRA BATISTA, ROBERTA CRISTELLI FONSECA, DENISE ALVES PEREZ, DÉBORA MOREIRA ALVARENGA, DENISE CARMONA CARA C - 18 INDUCTION OF EOSINOPHIL APOPTOSIS BY HYDROGEN PEROXIDE PROMOTES THE RESOLUTION OF ALLERGIC INFLAMMATORY RESPONSE. ALESANDRA CORTE REIS, RAYSSA MACIEL ATHAYDE, THIAGO VINICIUS ÁVILA, JULIANA PRISCILA VAGO, MILENE ALVARENGA RACHID, MAURO MARTINS TEIXEIRA1, VANESSA PINHO C - 35 TEMPORAL EVALUATION OF THE METABOLIC AND IMMUNOLOGICAL CONSEQUENCES IN AN EXPERIMENTAL MODEL OF FOOD ALLERGY. NATHÁLIA VIEIRA BATISTA, RAFAELA VAZ SOUSA PEREIRA, ROBERTA CRISTELLI FONSECA, ADALIENE VERSIANI MATOS FERREIRA, DENISE CARMONA CARA C - 19 EVALUATION OF LACTOCOCCUS LACTIS SECRETING OR NOT HSP 65 TREATMENT AS STRATEGY OF FOOD ALLERGY IMMUNOMODULATION. DENISE ALVES PEREZ, DÉBORA MOREIRA ALVARENGA, NATHÁLIA VIEIRA BATISTA, RAFAELA VAZ DE SOUZA PEREIRA, ROBERTA CRISTELLI FONSECA, ANA CRISTINA GOMES SANTOS, ANA MARIA CAETANO DE FARIA, ANDERSON MIYOSHI, GUSTAVO BATISTA DE MENEZES, DENISE CARMONA CARA C - 36 INFLUENCE OF PANCREATIC ACINAR CELL NECROSIS ON STELLATE CELL PROLIFERATION IN VITRO. BURKHARD KRUEGER, KRISTINA GEISSLER C - 20 EFFECTS OF CHLOROQUINE TREATMENT ON THE MOUSE IMMUNE SYSTEM. RODOLFO THOMÉ, ALESSANDRO DOS SANTOS FARIAS, FÁBIO TRINDADE MARANHÃO COSTA, LIANA VERINAUD C - 37 PARTICIPATION OF PROINFLAMMATORY CYTOKINES AND CELL MIGRATION IN THE ANTI-INFLAMMATORY EFFECTS OF A SULFATED POLYSACCHARIDE FRACTION EXTRACTED FROM THE MARINE ALGAE GRACILARIA CAUDATA IN MICE. LUCAS ANTONIO DUARTE NICOLAU., RENAN OLIVEIRA SILVA, LARISSE TAVARES LUCETTI, ANA PAULA MACEDO SANTANA, ANDRE LUIZ DOS REIS BARBOSA, KAROLINE SABÓIA ARAGÃO, RONALDO DE ALBUQUERQUE RIBEIRO, MARCELLUS HENRIQUE LOIOLA PONTE DE SOUZA, JAND-VENES ROLIM MEDEIROS C - 21 EFFECT OF LOW LEVEL LASER THERAPY ON THE VIABILITY OF MACROPHAGES ACTIVATED WITH LPS. LUIZA GABRIELA BARROS, NADHIA HELENA COSTA SOUZA, JANE PATRÍCIA DE MELO HAYASHI, RAQUEL AGNELLI MESQUITA FERRARI, SANDRA KALIL BUSSADORI, KRISTIANNE PORTA SANTOS FERNANDES C - 38 NITRIC OXIDE REDUCES ALENDRONATE-INDUCED GASTRIC DAMAGE IN RATS: ROLE OF CYTOKINES AND OXIDATIVE STRESS. LUCAS ANTONIO DUARTE NICOLAU., RENAN OLIVEIRA SILVA, NATÁLIA RODRIGUES D’ARC COSTA, LARISSE TAVARES LUCETTI, ANDRE LUIZ DOS REIS BARBOSA, ANA PAULA MACEDO 98 SANTANA, KAROLINE SABÓIA ARAGÃO, RONALDO DE ALBUQUERQUE RIBEIRO, MARCELLUS HENRIQUE LOIOLA PONTE DE SOUZA, JAND-VENES ROLIM MEDEIROS C - 39 HOW DOES THE PLATELET-ACTIVATING FACTOR ACT IN FOOD ALLERGY? ROBERTA CRISTELLI FONSECA, NATHÁLIA VIEIRA BATISTA, RAFAELA VAZ SOUSA PEREIRA, DENISE ALVES PEREZ, DÉBORA MOREIRA ALVARENGA, VANESSA PINHO, DENISE CARMONA CARA C - 40 IMPROVEMENT OF SKIN WOUND HEALING BY PARENTERAL INJECTION OF TOLERATED PROTEINS INTO ORALLY TOLERANT MICE. RAQUEL ALVES COSTA, LIANA BIAJOLLI O. MATOS, GREGORY THOMAS KITTEN, NELSON MONTEIRO VAZ, CLÁUDIA ROCHA CARVALHO C - 41 EFFECTS OF LOW LEVEL LASER THERAPY ON THE CREATINE KINASE ACTIVITY IN C2C12 CELLS. JEAN LUCAS PARPINELLI BARBOSA, MIKAELE TAVARES SILVA, PAOLA PELEGRINELI ARTILHEIRO, KRISTIANNE PORTA SANTOS FERNANDES, SANDRA KALIL BUSSADORI, RAQUEL AGNELLI MESQUITA-FERRARI C - 42 MOLECULAR FEATURES OF ANEMIA AND OBESITY. THAÍS DA FONTE FARIA, SIMONE VARGAS DA SILVA, THEREZA CHRISTINA BARJA FIDALGO, MARTA CITELLI DOS REIS C - 43 IMPROVEMENT OF SKIN WOUND HEALING BY PARENTERAL INJECTION OF A REGULAR DIET COMPONENT (ZEIN). THIAGO CANTARUTI ANSELMO, RAQUEL ALVES COSTA, NELSON MONTEIRO VAZ, CLAUDINEY MELQUIADES RODRIGUES, KÊNIA SOARES DE SOUZA, CLAUDIA ROCHA CARVALHO C - 44 ALPHA-MELANOCYTE STIMULATING HORMONE REDUCE INFLAMMATORY CELL COUNT AFTER EXCISIONAL CUTANEOUS WOUND. KÊNIA SOARES DE SOUZA, GERALDO MAGELA DE AZEVEDO JUNIOR, RAQUEL ALVES COSTA, CLAUDINEY MELQUIADES RODRIGUES, THIAGO CANTARUTI ANSELMO, NELSON MONTEIRO VAZ, CLÁUDIA ROCHA CARVALHO C - 45 TOLL LIKE RECEPTORS 2 AND 4 LEAD TO CARDIOMYOCYTE HYPERTROPHY IN VITRO. FERNANDA GAISLER DA SILVA, MARCELA SORELLI CARNEIRO RAMOS C - 46 RENAL ISCHEMIA/REPERFUSION INDUCED CARDIAC HYPERTROPHY IN MICE: INCREASED GENE EXPRESSION OF HSP 60 AND 70, TOLL-LIKE RECEPTOR LIGANDS. MAYRA TRENTIN SONODA, MARCELA SORELLI CARNEIRO RAMOS C - 47 EFFECTS OF OLEIC AND LINOLEIC ACIDS ON KERATINOCYTES. GILSON MASAHIRO MURATA, RUI CURI, ELAINE HATANAKA C - 48 IMPACT OF PERIODONTAL STATUS ON MUSCLE REPAIR PROCESS OF SEDENTARY AND TRAINED WISTAR RATS. BÁRBARA CAPITANIO DE SOUZA, MARCELO LAZZARON LAMERS, ALESSANDRA MAGNUSSON, MARCELO EKMAN RIBAS, ANDRÉ LUIZ LOPES, BRUNO COSTA TEIXEIRA C - 49 APOLIPOPROTEIN E COG 133 MIMETIC PEPTIDE ATTENUATES 5FLUOROURACIL-INDUCED INTESTINAL MUCOSITIS IN VITRO AND IN VIVO. ORLEÂNCIO GOMES RIPARDO DE AZEVEDO, JARDLON ALBINO COSTA, CELINA VIANA DE ARAÚJO, HERENE BARROS MIRANDA LUCENA, ROBERTO CÉSAR P. LIMAJÚNIOR, RENATO ANDRÉ C. OLIVEIRA, BRUNA CASTRO OLIVEIRA, MICHEL P. VITEK, RICHARD L. GUERRANT, RONALDO ALBUQUERQUE RIBEIRO, DEYSI VIVIANA T. WONG, TIÊ BEZERRA COSTA, SNJEZANA ZALA-MILATOVIC, REINALDO BARRETO ORIÁ C - 50 EFFECTS OF ZINC SUPPLEMENTATION ON GROWTH, INTESTINAL BACTERIAL TRANSLOCATION, AND PRO-INFLAMMATORY CYTOKINES IN WISTAR RATS CHALLENGED BY UNDERNUTRITION AND LACTOSE-INDUCED OSMOTIC DIARRHEA. ANITA MAYARA FEITOSA SANTOS, CAMILA DE ALBUQUERQUE ALMEIDA, PRISCILA BRISENO FROTA, SAID GONÇALVES DA CRUZ FONSECA, ÍTALO LEITE FIGUEIREDO, KAROLINE SABOIA ARAGÃO, CARLOS EMANUEL C. MAGALHÃES, CIBELE BARRETO MANO DE CARVALHO, REINALDO BARRETO ORIÁ C - 51 INFLIXIMAB ATTENUATES BONE RESORPTION AND INFLAMMATORY OSTEOLYSIS IN A MODEL OF EXPERIMENTAL PERIODONTITIS IN WISTAR RATS. RAKEL DE CASTRO EVANGELISTA, DAVI DA CUNHA GONÇALVES, ANITA MAYARA FEITOSA SANTOS, RAFAEL REIS DA SILVA, GERLY ANNE DE CASTRO BRITO, RENATA DE CARVALHO LEITÃO, REINALDO BARRETO ORIÁ C - 52 MACROPHAGE EFFECTS IN THE NEPHROTOXICITY PROCESS CAUSED BY IMMUNOSUPPRESSANT CYCLOSPORINE A: EXPRESSIONS OF TNFALFA AND ANNEXIN A1. AYLA BLANCO POLTRONIERI, CARLA PATRÍCIA CARLOS, EMMANUEL DE ALMEIDA BURDMANN, SONIA MARIA OLIANI C - 53 A SNAKE VENOM SECRETED PHOSPHOLIPASE A2 CROSSTALKS WITH INTRACELLULAR PHOSPHOLIPASES TO INDUCE MAST CELLS (MCS) DEGRANULATION. MARLOS CORTEZ SAMPAIO, BRUNO LOMONTE, JOSÉ MARIA GUTIERREZ, CATARINA TEIXEIRA C - 54 EVALUATION OF MACROPHAGE INFILTRATION IN ADIPOSE TISSUE OF EX-OBESE PATIENTS AND ROLE OF MESENCHYMAL STROMAL CELLS ON THE IMMUNOPHENOTYPE OF MACROPHAGES. DAIANA VIEIRA LOPES, CESAR S. CLÁUDIO-DA-SILVA, MARCELO C.A. SOUZA, MORENA P. DIAS, HÉLIO DOS SANTOS DUTRA, RADOVAN BOROJEVIC, CHRISTINA MAEDA TAKIYA, M. ISABEL DORIA ROSSI C - 55 COMPARATIVE EFFECTS OF EPA AND DEFLAZACORT ON DYSTROPHIN-DEFICIENT MUSCLE FIBERS OF THE MDX MICE. LETICIA MONTANHOLI APOLINARIO, SAMARA CAMAÇARI DE CARVALHO, HUMBERTO SANTO NETO, MARIA JULIA MARQUES C - 56 EFFECT OF PHOTOBIOMODULATION USING DIFFERENT ENERGY DENSITIES ON PROLIFERATION OF C2C12 SKELETAL MUSCLE CELLS DURING DIFFERENTIATION PROCESS. MIKAELE TAVARES DA SILVA, JEAN LUCAS PARPINELLI BARBOSA, PAOLA PELEGRINELI ARTILHEIRO, SANDRA KALIL BUSSADORI, KRISTIANNE PORTA SANTOS FERNANDES, RAQUEL AGNELLI MESQUITA-FERRARI C - 57 A PHOSPHOLIPASE A2 (CBR) ISOLATED FROM VENOM OF CROTALUS DURISSUS RURUIMA INDUCES LIPID BODY (LB) FORMATION IN MACROPHAGES. ANA EDUARDA ZULIM DE CARVALHO, KARINA CRISTINA GIANNOTTI, ELBIO LEIGUEZ JUNIOR, MÁRCIO HIDEKI MATSUBARA, CONSUELO LATORRE FORTES DIAS, MARIA CRISTINA DOS SANTOS, CATARINA TEIXEIRA C - 58 EFFECT OF ACTIVATION ON THE VIABILITY OF J774 MACROPHAGES. JANE PATRICIA DE MELO HAYASHI, NADHIA HELENA COSTA SOUZA, LUIZA GABRIELA BARROS, RAQUEL AGNELLI MESQUITA FERRARI, SANDRA KALIL BUSSADORI, KRISTIANNE PORTA SANTOS FERNANDES C - 59 LIPID BODIES FORMATION INDUCED BY A SNAKE VENOM PHOSPHOLIPASE A2 (CB) IS RELATED TO INCREASED PGE2 BIOSYNTHESIS IN MACROPHAGES. KARINA CRISTINA GIANNOTTI, ELBIO LEIGUEZ JUNIOR, NEIDE GALVÃO DO NASCIMENTO, ANA EDUARDA ZULIM DE CARVALHO, CONSUELO LATORRE FORTES-DIAS, RENATA HAGE, CATARINA TEIXEIRA C - 60 EFFECTS OF EICOSAPENTAENOIC ACID (EPA) ON M1 AND M2 MACROPHAGE POPULATIONS IN SKELETAL MUSCLES OF THE MDX MICE. SAMARA CAMAÇARI DE CARVALHO, LETÍCIA MONTANHOLI APOLINÁRIO, HUMBERTO SANTO NETO, SELMA MARIA MICHELIN MATHEUS, MARIA JÚLIA MARQUES C - 61 THE DEVELOPMENT OF ENDOMETRIOTIC LESIONS IN HETEROLOGOUS MODEL USING MICE THAT EXPRESS A GREEN FLUORESCENT PROTEIN (GFP): ANALYSIS OF THE ANGIOGENESIS PROCESSES. JOÃO MARCOS PEREIRA SANTOS, THAIS ANGELI GAMBA, KARINA CRISTINA RODRIGUEZ BAPTISTA, ANTÔNIO PALUMBO, LEONARDO BOLDRINI, RÔMULO MEDINA MATOS, JAMILA ALESSANDRA PERINI, LUIZ EURICO NASCIUTTI, DANIEL ESCORSIM MACHADO C - 62 EFFECT OF LOW LEVEL LASER THERAPY ON THE PROLIFERATION OF INFLAMMATORY MACROPHAGES. NADHIA HELENA COSTA SOUZA, LUIZA GABRIELA BARROS, JANE PATRÍCIA DE MELO HAYASHI, RAQUEL AGNELLI MESQUITA FERRARI, DANIELA DE FÁTIMA TEIXEIRA DA SILVA, SANDRA KALIL BUSSADORI, KRISTIANNE PORTA SANTOS FERNANDES C - 63 DEVELOPMENT OF IN VITRO ALTERNATIVE METHODS TO PREDICT ALLERGENIC POTENTIAL OF CHEMICAL AGENTS. JANE ZVEITER DE MORAES, VANESSA M. SÁ-ROCHA, JANE ZVEITER DE MORAES C - 64 STUDY OF ANTI-INFLAMMATORY ACTIONS OF METHYL GALLATE. TATIANA ALMEIDA PÁDUA, BIANCA SUELEN DA SILVA CRUZ DE ABREU, MARCIA VIDAL DE CARVALHO, MARIA RAQUEL FIGUEREIDO, MARIA DAS GRAÇAS HENRIQUES, ELAINE CRUZ ROSAS C - 65 INCREMENT OF MAST CELLS, NEOVASCULARIZATION AND ACTIVATION OF NFΚB IN THE ACHILLES TENDON OF RATS WITH EXPERIMENTAL DIABETES. RODRIGO RIBEIRO DE OLIVEIRA, YURI RODRIGUES ROCHA, ALLYSSON BRUNO RAPHAEL BRAGA, GERLY ANNE DE CASTRO BRITO, LUIZ EURICO NASCIUTTI C - 66 AMYLOID FIBRILS INDUCE NEUTROPHIL EXTRACELLULAR TRAPS (NETS) FORMATION BY HUMAN NEUTROPHILS AND ARE DIGESTED BY ELASTASE. ANDERSON GUIMARÃES BAPTISTA COSTA, ESTEFÂNIA PEREIRA CARDOSO AZEVEDO, GUILHERME TOREZANI, CAROLINA AZEREDO BRAGA, FERNANDO LUCAS PALHANO, JEFFERY KELLY, ELVIRA SARAIVA, DEBORA FOGUEL C - 67 ATTRACTING CHEMOKINES EXPRESSION FOR POLYMORPHONUCLEAR CELLS IN WHITE ADIPOSE TISSUE FROM CANCER CACHEXIA RATS. FELIPE HENRIQUES, FELIPE FRANCO, PAMELA KNOB, KALTINAITE BENETON, RODRIGO XAVIER, CLAUDIO SHIDA, ROGERIO SERTIÉ, SIDNEY PERES, MIGUEL BATISTA JR C - 68 BOTHROPIC TOXIN MODULATES INFLAMMATORY ANGIOGENESIS IN MICE. PUEBLA CASSINI VIEIRA, AMANDA VIEIRA, SAULO ANTONIO GOMES FILHO, SIMONE RAMOS DECONTE, FABIO DE OLIVEIRA, FERNANDA DE ASSIS ARAÚJO C - 69 ATLA, AN ASPIRIN-TRIGGERED LIPOXIN A4 SYNTHETIC ANALOG, IN THE TREATMENT OF FIBROTIC EFFECTS OF BLEOMYCIN-INDUCED LUNG DAMAGE. RAFAEL DE FREITAS GUILHERME, DEBORA GONÇALVES XISTO, PATRICIA RIEKEN MACEDO ROCCO, IOLANDA MARGHERITA FIERRO, CLAUDIO DE AZEVEDO CANETTI, CLAUDIA FARIAS BENJAMIM C - 70 THE ROLE OF LAMININ POLYMER IN SPLENIC DENDRITIC CELLS. LEANDRO LADISLAU ALVES, AMANDA REGINA DA FÉ, STEVEN L KUNKEL, THEREZA CHRISTINA BARJA FIDALGO, WILSON SAVINO, CLAUDIA FARIAS BENJAMIM C - 71 ANTI-INFLAMMATORY EFFECTS OF GALECTIN-1 PROTEIN ON LIPOPOLYSACCHARIDE-CHALLENGED CULTURED HUMAN RETINAL PIGMENT EPITHELIAL CELLS. NATHÁLIA MARTINS SONEHARA, LAILA TONIOL CARDIN, CRISTIANE DAMAS GIL, SONIA MARIA OLIANI C - 72 INVESTIGATION OF MAST CELL HETEROGENEITY AND EXPRESSION OF ANTI-INFLAMMATORY PROTEIN ANNEXIN A1 IN ENDOMETRIOSIS. RUBENS DE PAULA JUNIOR, POATAN DA SILVA PINOTI, ANTONIO HELIO OLIANI, DENISE CRISTINA MOS VAZ, SOLANGE CORREA GARCIA P D”AVILA, SONIA MARIA OLIANI, CRISTIANE DAMAS GIL 99 C - 73 EFECT OF STEROID AND NON-STEROID ANTI-INFLAMMATORY COMPOUNDS ON S100B SECRETION IN PRIMARY ASTROCYTE CULTURES EXPOSED OR NOT TO LPS. ELISA NEGRI, CAROLLINA FRAGA DA RÉ, FABIANA GALLAND, MARIA CRISTINA GUERRA, MARINA CONCLI LEITE, CARLOS ALBERTO GONÇALVES C - 74 LOVASTATIN DECREASES NEUROINFLAMMATION AND PREVENTS COGNITIVE IMPAIRMENT AFTER CEREBRAL MALARIA. PATRICIA ALVES REIS, TATHIANY IGREJA DA SILVA, EDSON FERNANDES DE ASSIS, PATRICIA TORRES BOZZA, FERNANDO AUGUSTO BOZZA, HUGO CAIRE DE CASTRO FARIA NETO C - 75 UNRAVELING A NEW CELLULAR MECHANISM BEHIND TRANSTHYRETIN-RELATED LEPTOMENINGEAL AMYLOIDOSIS USING AS MODEL A HIGHLY UNSTABLE TRANSTHYRETIN MUTANT. ESTEFANIA PEREIRA CARDOSO AZEVEDO, FERNANDO PALHANO, MORGANA SOBRINHO, LUCIANA ROMÃO, FLÁVIA LIMA, VIVALDO MOURA NETO, DÉBORA FOGUEL C - 76 SRC KINASE RELAYS INFLAMMATORY INFORMATION TO EXERT A FINE-TUNED CONTROL OF MICROGLIA ACTIVATION. RENATO SOCODATO, CAMILA CABRAL PORTUGAL, VIVIAN COREIXAS, ERICK CORREIA LOIOLA, FILIPA DOMINGUES, ANA RAQUEL SANTIAGO, ROBERTO PAES DE CARVALHO, JOÃO B RELVAS, FRANCISCO AMBRÓSIO C - 77 LIPID-LADEN MULTILOCULAR CELLS: DISTRIBUTION, MORPHOLOGICAL AND PHENOTYPICAL CHARACTERIZATION OF A NEGLECTED CELL COMPONENT IN THE THYMIC MICROENVIRONMENT OF AGING MICE. LARISSA GUTMAN PARANHOS LANGHI, LEONARDO RODRIGUES DE ANDRADE, RADOVAN BOROJEVIC, VALÉRIA DE MELLO COELHO C - 78 SODIUM VITAMIN C CO-TRANSPORTER-2 (SVCT-2) INTERNALIZATION UNDER PRO-INFLAMMATORY CONDITIONS IS ASSOCIATED WITH MICROGLIA ACTIVATION. CAMILA CABRAL PORTUGAL, RENATO SOCODATO, VIVIAN COREIXAS, ERICK CORREIA LOIOLA, ANA RAQUEL SANTIAGO, ROBERTO PAES DE CARVALHO, FRANCISCO AMBRÓSIO C - 79 OBATOCLAX DECREASES NEUTROPHILS IN THE MODEL OF ANTIGEN-INDUCED ARTHRITIS (AIA) IN MICE. WILLIAM ANTÔNIO GONÇALVES, FERNANDO LOPES, MAURO MARTINS TEIXEIRA, VANESSA PINHO C - 80 EXPRESSION OF PLIN2 AND FORMATION OF LIPID BODIES INDUCED BY DISTINCT SPECIES OF BOTHROPS SNAKE VENOM IN LEUKOCYTES. NEIDE GALVÃO DO NASCIMENTO, ELBIO LEIGUEZ, KARINA CRISTINA GIANNOTTI, MARIANA VIANA, CATARINA TEIXEIRA C - 81 LITHOTHAMNION MUELLERI: A RED ALGAE WHICH REDUCES THE INFLAMMATORY RESPONSE ASSOCIATED WITH GRAFT-VERSUS-HOST DISEASE WITHOUT IMPAIR THE BENEFICIAL RESPONSE OF GRAFT-VERSUS-LEUKEMIA. BARBARA M. REZENDE, PRISCILA T. T. BERNARDES, CAROLINA B. RESENDE, LÍVIA BARROSO, ROSA M. E. ARANTES, DANIELLE G. SOUZA, MAURO M. TEIXEIRA, MARINA G. M. CASTOR, VANESSA PINHO C - 82 BAP1 METALLOPROTEINASE STIMULATES B TYPE SYNOVIOCYTES TO PRODUCE PGE2 AND REQUIRES COX-2 AND EP4 RECEPTORS TO THIS EFFECT. MARIANA DO NASCIMENTO VIANA, CRISTINA MARIA FERNANDES, ELBIO LEIGUEZ JUNIOR, MÁRCIO HIDEKI MATSUBARA, JOSE MARIA GUTIÉRREZ, CATARINA DE FÁTIMA PEREIRA TEIXEIRA C - 83 HIGHER ACTIVATED POPULATION OF T LYMPHOCYTES AND DECREASED T REGULATORY CELL POPULATION IN DMD PATIENTS. LUCIANA RODRIGUES CARVALHO BARROS, FERNANDA PINTO MARIZ, MARIANA FERREIRA VEGAS, ALEXANDRA QUEIROZ PRUFER ARAUJO, MARCIA RIBEIRO, MARIA DO CARMO SOARES CUNHA, WILSON SAVINO C - 84 ANTI-INFLAMMATORY EFFECTS OF MAYTENUS ILICIFOLIA MART. EX REISSEK IN SWISS MICE. JANAÍNA VIEIRA BELUSSO, FABÍOLA REGINA BREDA, CARLA GIANE LOSS, ARNO ERNESTO HOFMANN JUNIOR, SILVANE SOUZA ROMAN C - 85 ROLE OF CHEMOKINE RECEPTOR CCR4 AND REGULATORY T CELLS IN WOUND HEALING IN MICE. JANAINA DE BARROS FIGUEIREDO, PAULA ALVARENGA BORGES, ARIANE RENNÓ BROGLIATO, JANAINA LIMA GEORGII, CYNTIA PECLI E SILVA, STEVEN L. KUNKEL, CLAUDIA FARIAS BENJAMIM C - 86 INVOLVEMENT OF IMMUNE RESPONSE IN THE RENAL INJURY INDUCED BY SEVERE SEPSIS IN MICE. AMANDA REGINA DA FÉ, LEANDRO LADISLAU ALVES, CYNTIA PECLI E SILVA, RAFAEL DE FREITAS GUILHERME, STEVEN L. KUNKEL, CLAUDIA FARIAS BENJAMIM, IOLANDA MARGHERITA FIERRO C - 87 WOUND-HEALING ASSESSMENT OF RUTA GRAVEOLENS L. (ARRUDA) EXTRACT IN WISTAR RAT SKIN. JANAÍNA VIEIRA BELUSSO, LUANA RORIG GALLI, CAMILA ZANELLA, GABRIELA GIORDANA LORENZON ALVES, ROGÉRIO LUIS CANSIAN, SILVANE SOUZA ROMAN C - 88 PULMONARY FUNCTION, OXIDATIVE STRESS AND INFLAMMATORY MARKERS IN LPS-INDUCED ACUTE LUNG INJURY: DIFFERENTIAL EFFECTS OF ATORVASTATIN, PRAVASTATIN AND SIMVASTATIN. ADRIANA CORREA MELO, LARISSA A. SILVA NETO, JACKSON N. ALVES, MARINA V. BARROSO, DENISE M. CARDOSO, ALAN A. LOPES, RÔMULO PINTO, RENATA T. NESI, EDUARDO TAVARES L. TRAJANO, GIOVANNA M. CARVALHO, WALTER ARAÚJO ZIN, LUIS CRISTÓVÃO PORTO, SAMUEL SANTOS VALENCA C - 89 THE ANTI-INFLAMMATORY ROLE OF ANNEXIN A1 PROTEIN IN HUMAN RETINAL PIGMENT EPITHELIUM (ARPE-19) AFTER ENDOTOXEMIA. KALLYNE KIOKO MIMURA, CRISTIANE DAMAS GIL, SONIA MARIA OLIANI C - 90 IMPAIRMENT HEALING OF DIABETIC WOUNDS IS IMPROVED BY ORAL ADMINISTRATION OF ANTIOXIDANTS. ANA FLÁVIA MARÇAL PESSOA, JULIANA DA COSTA FLORIM, HOSANA G RODRIGUES, MARCELO L LAMERS, RUI CURI, MARINILCE FAGUNDES DOS SANTOS C - 91 CELL-CELL INTERACTIONS BETWEEN CHONDROCYTES AND SYNOVIOCYTES LIKE CELLS. SAMYLLA MIRANDA MONTE, CAMILA BASILE CARBALLO C - 92 CELLULAR AND MOLECULAR MECHANISMS OF ANTIINFLAMMATORY EFFECT OF THE SIARESINOLIC ACID. ALMAIR FERREIRA DE ARAÚJO, JAMYLLE NUNES DE SOUZA FERRO, ANDERSON MARQUES DE OLIVEIRA, LÚCIA MARIA CONSERVA, EMILIANO DE OLIVEIRA BARRETO C - 93 ANALYSIS OF RETINOBLASTOMA PHOSPHORYLATION AND NUCLEAR Β-CATENIN ACCUMULATION HELPS THE DIFFERENTIAL DIAGNOSIS BETWEEN CROHN’S DISEASE AND ULCERATIVE COLITIS. ROSSANA COLLA SOLETTI, NATHASSYA ACCIOLY LINS VIDAL RODRIGUES, DEBORAH BIASOLI, VIVALDO MOURA NETO, HEITOR SIFFERT PEREIRA DE SOUZA, HELENA LOBO BORGES C - 94 PRO-INFLAMMATORY EFFECTS OF THE P2X7 RECEPTOR IN SEPSIS. PATRICIA TEIXEIRA SANTANA, LUIZ EDUARDO BAGGIO SÁVIO, CLÁUDIA FARIA BENJAMIM, CHRISTINA MAEDA TAKIYA, ROBSON COUTINHO SILVA C - 95 INHIBITORY EFFECT OF CURINE ON EOSINOPHIL ACTIVATION AND AIRWAY HYPER-RESPONSIVENESS. JAIME RIBEIRO FILHO, ADRIANA VIEIRA-DEABREU, ANDREA SURRAGE CALHEIROS, JULIANA ALVES AZEREDO, CELIDARQUE DA SILVA DIAS, MÁRCIA REGINA PIUVEZAM, PATRÍCIA TORRES BOZZA C - 96 STEM CELL FACTOR INDUCES PIECEMEAL DEGRANULATION IN HUMAN EOSINOPHIL. KENNEDY BONJOUR DE OLIVEIRA FERREIRA, FELIPE FERRAZ DIAS, ANN M. DVORAK, PETER F. WELLER, ROSSANA CORREA NETTO MELO C - 97 INVOLVEMENT OF PPARGAMMA ON LIPID BODY FORMATION AND HOST IMMUNE RESPONSE DURING INFECTION BY TRYPANOSOMA CRUZI. LÍVIA TEIXEIRA, HELOÍSA D'ÁVILA, CÉLIO GERALDO FREIRE DE LIMA, ROSSANA CORREA NETTO DE MELO, PATRÍCIA TORRES BOZZA C - 98 IMPACT OF THE ABSENCE OF GALECTIN-3 ON THE COURSE OF EXPERIMENTAL INFECTION WITH TRYPANOSOMA CRUZI. DANIELLE SILVA DOS SANTOS, JULIANA BARRETO DE ALBUQUERQUE, LUIZ RICARDO BERBERT, LANDI V.C. GUILLERMO, WILSON SAVINO, JULIANA DE MEIS, DÉA M. S. VILLA-VERDE C - 99 HEMATOLOGICAL PARAMETERS OF CHICKENS TREATED WITH NITRIC OXIDE INHIBITOR AND INFECTED WITH PLASMODIUM GALLINACEUM. BARBARELLA DE MATOS MACCHI, FARLEN JOSÉ BEBBER MIRANDA, FERNANDA SILVA DE SOUZA, EULÓGIO CARLOS QUEIROZ DE CARVALHO, ANTÔNIO PEIXOTO ALBERNAZ, JOSÉ LUIZ MARTINS DO NASCIMENTO, RENATO AUGUSTO DAMATTA C - 100 NEUROPROTECTIVE EFFECTS OF MELATONIN SYNTHESIZED BY CEREBELLAR GRANULE CELLS CULTURES. DAIANE GIL FRANCO, ADRIESSA APARECIDA DOS SANTOS, REGINA P. MARKUS C - 101 ANTIOXIDANT AND CHEMICAL PROFILE OF BRAZILIAN PROPOLIS: AN IN VITRO STUDY. ALAN DE AGUIAR LOPES, LARISSA ALEXSANDRA SILVA-NETO, THIAGO DOS SANTOS FERREIRA, KARLA MARIA PEREIRA PIRES, MANUELLA LANZETTI, RENATA TISCOSKI NESI, ARI MIRANDA SILVA, ANTONIO JORGE RIBEIRO DA SILVA, SAMUEL DOS SANTOS VALENÇA, LUÍS CRISTÓVÃO DE MORAES SOBRINO PÔRTO C - 102 BLOCKAGE OF EXTRACELLULAR ATP/ADP SIGNALING REDUCES ACETAMINOPHEN-INDUCED LIVER DAMAGE. JAYANE LAIS DIAS QUINTÃO, SYLVIA STELLA AMARAL, PEDRO ELIAS MARQUES, DANIELE ARAÚJO PIRES, GUSTAVO BATISTA MENEZES C - 103 CARDIAC HYPERTROPHY INDUCED RENAL ISCHEMIA/REPERFUSION: GENE EXPRESSION ANALYSIS OF INFLAMMATORY CELLS PROLIFERATION IN HEART TISSUE. KARINA KAORI NAKAMA, MARCELA SORELLI CARNEIRO RAMOS C - 104 A SNAKE VENOM SECRETED PHOSPHOLIPASE A2 INDUCES PPAR-Γ AND –Β EXPRESSION AND ACTIVATION IN MACROPHAGES: IMPLICATION IN PLIN2 PROTEIN EXPRESSION. ELBIO LEIGUEZ, KARINA CRISTINA GIANNOTTI, JOSÉ MARIA GUTIÉRREZ, BRUNO LOMONTE, CATARINA TEIXEIRA C - 105 AMYLOID-BETA PEPTIDE TRIGGERS NUCLEAR FACTOR KAPPA B SIGNALING PATHWAY IN RAT PINEAL GLANDS. ERIKA CECON, PEDRO AUGUSTO CARLOS MAGNO FERNANDES, EDUARDO KOJI TAMURA, REGINA PEKELMANN MARKUS C - 106 EVALUATION OF THE EFFECT OF PROPIONIBACTERIUM ACNES ON THE SURVIVAL OF ANIMALS WITH LETHAL SEPSIS. SAMARA KELLY MENDONÇA DE OLIVEIRA, JOSÉ BRUNO NUNES FERREIRA DA SILVA, LARISSA CARDOSO CORRÊA DE ARAÚJO, JACIANA DOS SANTOS AGUIAR, TERESINHA GONÇALVES DA SILVA C - 107 MECHANISMS INVOLVED IN LIPID BODY FORMATION AND AUTOPHAGY DURING EXPERIMENTAL INFECTION BY MYCOBACTERIUM BOVIS BCG IN MICE. HELOISA D`AVILA DA SILVA BIZARRO, NATÁLIA ROBERTA ROQUE, ALINE APARECIDA ASSIS, DOUGLAS MOREIRA DE ARAÚJO, GABRIEL SANTOS CRUZ RODRIGUES, SÍLVIA LUCENA LAGE, PATRÍCIA ELAINE ALMEIDA, ROSSANA. CORREA NETTO MELO, HUGO C. CASTRO-FARIA-NETO, CLARISSA MAYA MONTEIRO, PATRÍCIA TORRES BOZZA 100 C - 108 ROLE OF CAVEOLIN-1 IN THE REGULATION OF PRODUCTION OF INFLAMMATORY MEDIATORS IN PERITONEAL MACROPHAGES.CECÍLIA JACQUES GONÇALVES DE ALMEIDA, CAROLINA DA PAZ ZAMPIER, MICHAEL P. LISANTI, PATRÍCIA T. BOZZA D-5 TESTES MORPHOLOGY AND SPERMATOGENESIS IN TELCHIN LICUS LICUS (LEPIDOPTERA: CASTNIIDAE) MONIQUE CAMPOS PEREIRA, DANIELA CARVALHO DOS SANTOS, ELTON LUIZ SCUDELER, ANA SILVIA GIMENES GARCIA, REINALDO JOSÉ DA SILVA C - 109 EARLY AND LATE ACUTE LUNG INJURY AND ITS ASSOCIATION WITH DISTAL ORGAN DAMAGE IN EXPERIMENTAL MALARIA. MARIANA CONCEIÇÃO DE SOUZA, JOHNATAS DUTRA SILVA, TATIANA ALMEIDA PADUA, VERA LUIZA CAPELOZZI, PATRICIA R. M. ROCCO, MARIA DAS GRAÇAS HENRIQUES D-6 REPRODUCTIVE DEVELOPMENT OF THE MALE OFFSPRING FROM RATS TREATED WITH CARBAMAZEPINE DURING PREGNANCY SAMARA URBAN DE OLIVA, TAIZA STUMPP, SANDRA MARIA MIRAGLIA C - 110 SNAKE VENOM TOXIN BMOOMP-ALFA-I INHIBITS ANGIOGENESIS IN MURINE MODELS. SAULO ANTONIO GOMES FILHO, PUEBLA CASSINI VIEIRA, SIMONE RAMOS DECONTE, AMANDA VIEIRA, FABIO DE OLIVEIRA, FERNANDA DE ASSIS ARAÚJO C - 111 NO AND IL-1Β PRODUCTION BY MURINE MACROPHAGES TREATED WITH LECTINS CMOL AND WSMOL. LARISSA CARDOSO CORRÊA DE ARAÚJO, JACIANA DOS SANTOS AGUIAR, MARIA DO DESTERRO RODRIGUES, JEYCE KELLE FERREIRA DE ANDRADE, LUANA CASSANDRA BREITENBACH BARROSO COELHO, TERESINHA GONÇALVES DA SILVA, PATRÍCIA MARIA GUEDES PAIVA C - 112 DOWN-MODULATION OF ACTIVATED HUMAN NEUTROPHIL BY LMW-FUCOIDAN. JOÃO ALFREDO DE MORAES, ANA CLARA FRONY, GENILSON RODRIGUES, CATHERINE BOISSON-VIDAL, CHRISTINA BARJA-FIDALGO C - 113 DIESEL PARTICLES (DEP) INDUCE MELATONIN SYNTHESIS BY MACROPHAGES THROUGH NF-KB ACTIVATION. CLAUDIA EMANUELE CARVALHO DE SOUSA, SANDRA MARCIA MUXEL, ALESSANDRA STRANIERI, MARIANGELA MACCHIONE, PAULO HILARIO NASCIMENTO SALDIVA, REGINA PEKELMANN MARKUS C - 114 CHARACTERIZATION OF GENE EXPRESSION IN CD14+CD16-, CD14+CD16+ AND CD14DIMCD16++ MONOCYTE SUBSETS IN OBESITY. MARIANA RENOVATO MARTINS, ESTELLE DEVEVRE, ELISE DALMAS, JEAN-LUC BOUILLOT, ARNAUD BASEDEVANT, WOLF-HERMAN FRIDMAN, THEREZA CHRISTINA BARJAFIDALGO, KARINE CLÉMENT, CATHERINE SAUTÈS-FRIDMAN, ISABELLE CREMER, CHRISTINE POITOU C - 115 EVALUATION OF THE EFFECT OF 5 AZA CYTIDINE ON THE WOUND HEALING IN WISTAR RATS. FABIANA DE SOUZA GOMES, LÍCIO AUGUSTO VELLOSO, CARLA EVELYN COIMBRA NUÑEZ, GABRIELA FREITAS PEREIRA DE SOUZA, MARIA HELENA MELO LIMA, RAFAEL DE MORAES PEDRO, ELIANA PEREIRA DE ARAÚJO C - 116 THE ROLE OF PURINERGIC P2X7 RECEPTORS IN MURINE SILICOSIS. LEONARDO MONÇÃO RIBEIRO, PATRICIA TEIXEIRA SANTANA, RADOVAN BOROJEVIC, CHRISTINA MAEDA TAKIYA, ROBSON COUTINHO SILVA C - 117 INCREASED LEPTIN RESPONSE AND INHIBITION OF APOPTOSIS IN THYMIC CELLS FROM YOUNG OFFSPRING SUBMITTED TO MATERNAL PROTEIN DEPRIVATION DURING LACTATION. SIMONE VARGAS DA SILVA, CAROLINA SALAMA, MARIANA RENOVATO MARTINS, EDWARD HELAL NETO, MARTA CITELLI, WILSON SAVINO, CHRISTINA BARJA-FIDALGO C - 118 SHORT- TERM TREATMENT WITH IL-10-PRODUCING LACTOCOCCUS LACTIS REDUCES SYSTEMIC IL-17 BUT DO NOT IMPROVE THE CLINICAL SIGNALS OF COLITIS.LUÍSA LEMOS DOS SANTOS, ANA CRISTINA GOMES-SANTOS, THAIS GARCIA MOREIRA, BERNARDO COELHO HORTA, ANDERSON MIYOSHI, DENISE CARMONA CARA, ANA MARIA CAETANO DE FARIA D – Cell Biology and Reproduction D1-D140 D-1 STRUCTURAL AND ULTRASTRUCTURAL ANALYSIS OF THE PLACENTA IN PREGNANT RATS FED WITH PROTEIN RESTRICTION DIET. HÉRCULES JONAS REBELATO, MARCELO AUGUSTO MARRETTO ESQUISATTO, PAULO PINTO JOAZEIRO, ROSANA CATISTI D-2 HYPERTHERMIC STRESS AFFECTS SPERMATOGENESIS EUPEMPHIX NATTERERI (ANURA: LEIUPERIDAE) GABRIELA BARONI LEITE, JULIANE SILBERSCHIMDIT FREITAS, LIA RAQUEL SOUZA DOS SANTOS, LILIAN FRANCOBELUSSI, CLASSIUS DE OLIVEIRA D-3 CHRONIC CAFFEINE INTAKE INCREASES ANDROGENIC STIMULI, EPITHELIAL CELL PROLIFERATION AND HYPERPLASIA IN RAT VENTRAL PROSTATE SÉRGIO LUIS FELISBINO, CAROLINA SAROBO, LIVIA MARIA LACORTE, MARCELA MARTINS, JAQUELINE CARVALHO RINALDI, IVAN JOSÉ VERCHETTI JUNIOR, ANDREI MOROZ, WELLERSON RODRIGO SCARANO, FLAVIA KARINA DELELLA D-4 SPERMATOGENESIS IN EUPEMPHIX NATTERERI (ANURA: LEIUPERIDAE): LATE RESPONSE TO LPS LARA SALGUEIRO DE GREGORIO, LILIAN FRANCO-BELUSSI, GABRIELA BARONI LEITE, JULIANE SILBERSCHMIDT FREITAS, CLASSIUS DE OLIVEIRA D-7 EVALUATION OF THE EFFECTS OF ABLATION OF TESTOSTERONE IN PROSTATIC COMPLEX OF ARTIBEUS PLANIROSTRIS (CHIROPTERA: PHYLLOSTOMIDAE). CINTIA CRISTINA ISICAWA PUGA, MATEUS RODRIGUES BEGUELINI, FABIANE FERREIRA MARTINS, ELIANA MORIELLE VERSUTE, PATRICIA SIMONE LEITE VILAMAIOR, SEBASTIÃO ROBERTO TABOGA D-8 EFFECT OF CARNITINE AND OF ETOPOSIDE ON SPERMATOGONIAL STEM/PROGENITOR CELLS OF RATS TREATED IN THE PREPUBERTAL PHASE. FATIMA KAZUE OKADA, TAIZA STUMPP, SANDRA MARIA MIRAGLIA D-9 COMPARATIVE STUDY OF BATS IN DORSAL PROSTATE REPRESENTATIVES OF THE SUBFAMILIES: GLOSSOPHAGINAE, CAROLLINAE E PHYLLOSTOMINAE (CHIROPTERA-PHYLLOSTOMIDAE) FABIANE FERREIRA MARTINS, CINTIA ISICAWA PUGA, MATEUS RODRIGUES BEGUELINI, PATRICIA SIMONE LEITE VILAMAIOR, ELIANA MORIELLE-VERSUTE, SEBASTIÃO ROBERTO TABOGA D - 10 INVOLVEMENT OF ABCB1 AND ABCC1 PROTEINS IN SEA URCHIN FERTILIZATION PROCESS. HELENA LIMA DA SILVA NETA, TALITTA DANTAS DE ARRUDA, ELIS TORREZAN GONÇALVES RAMALHO NITÃO, LUIS FERNANDO MARQUES-SANTOS D - 11 GERM CELLS RECOVERY IN IRRADIATED RAT TESTIS AFTER TREATMENT WITH ACYLINE - A GNRH ANTAGONIST - AND FLUTAMIDE – AN ANDROGEN RECEPTOR ANTAGONIST AMANDA VASCONCELOS DE ALBUQUERQUE, MARVIN L. MEISTRICH, GUNAPALA SHETTY, FERNANDA F.R.C.L. ALMEIDA, HÉLIO CHIARINI GARCIA D - 12 CHARACTERIZATION OF INTERCELLULAR JUNCTIONS IN FIBROBLASTS OF MOUSE PUBIC SYMPHYSIS DURING PREGNANCY AND POSTPARTUM VIVIANE DE SOUZA ROSA, SÍLVIO ROBERTO CONSONNI, MONICA MOREIRA, BIANCA CASTELUCCI, PAULO PINTO JOAZEIRO D - 13 CHANGES OF THE MOUSE INTERPUBIC TISSUE THROUGHOUT THE MIDST OF PREGNANCY GABRIELA TOGNINI SABA, GIULLIANA PETRI, JULIANA MORA VERIDIANO, OLGA MARIA DE TOLEDO CORREA D - 14 MORPHOMETRICAL, STEREOLOGICAL AND ULTRASTRUCTURAL STUDY OF GREEN PROPOLIS EFFECTS ON RAT EPIDIDYMIS CRISTINA CAPUCHO, FABRÍCIA DE SOUZA PREDES, JULIANA DE CASTRO MONTEIRO, RENATA BARBIERI, MARY ANNE HEIDI DOLDER, GRASIELA DIAS DE CAMPOS SEVERI-AGUIAR D - 15 EXTENSIVE MONONUCLEAR INFILTRATION AND POSTPARTUM MOUSE PUBIC SYMPHYSIS RECOVERY BIANCA GAZIERI CASTELUCCI, SÍLVIO ROBERTO CONSONNI, VIVIANE DE SOUZA ROSA, PAULO PINTO JOAZEIRO D - 16 HETEROCHROMATIN PATTERNS IN TRIATOMA WILLIAMI (HEMIPTERA, TRIATOMINAE) NATHÁLIA PAIVA PERERIA, PRISCILA PASQÜETTO MENDONÇA, KAIO CESAR CHABOLI ALEVI, ANNA CLAUDIA CAMPANER LIMA, JOÃO ARISTEU DA ROSA, MARIA TERCÍLIA VILELA DE AZEREDO OLIVEIRA D - 17 LONG-TERM EFFECTS OF DEVELOPMENTAL EXPOSURE TO BISPHENOL A (BPA) ON THE PROSTATE OF ADULT RATS: CHEMOPROTECTIVE EFFECT OF INDOLE-3-CARBINOL (I3C) JOYCE ZALOTTI BRANDT, LÍVIA TERESA RIBEIRO DA SILVEIRA, TONY FERNANDO GRASSI, WAGNER JOSÉ FÁVARO, RAQUEL FANTIN DOMENICONI, JANETE A. ANSELMO-FRANCI, JOSÉ EDUARDO BOZANO, LUIS FERNANDO BARBISAN, WELLERSON RODRIGO SCARANO D - 18 C-HETEROCHROMATIN PATTERN AND NUCLEOLAR ACTIVITY IN HOLOCENTRIC CHROMOSOMES OF TRIATOMA LENTI (HEMIPTERA: REDUVIIDAE) KAIO CESAR CHABOLI ALEVI, PRISCILA PASQÜETTO MENDONÇA, NATHÁLIA PAIVA PEREIRA, ANNA CLAUDIA CAMPANER LIMA, JOÃO ARISTEU DA ROSA, MARIA TERCÍLIA VILELA DE AZEREDO OLIVEIRA D - 19 COMPARATIVE CYTOGENETIC STUDY BETWEEN TRIATOMA VANDAE E TRIATOMA WILLIAMI (HEMIPTERA, TRIATOMINAE) NATHÁLIA PAIVA PERERIA, PRISCILA PASQÜETTO MENDONÇA, KAIO CESAR CHABOLI ALEVI, ANNA CLAUDIA CAMPANER LIMA, JOÃO ARISTEU DA ROSA, MARIA TERCÍLIA VILELA DE AZEREDO OLIVEIRA D - 20 NUCLEOLAR ACTIVITY IN SPERMATOGENESIS OF RHODNIUS BRETHESI (HEMIPTERA, TRIATOMINAE) PRISCILA PASQÜETTO MENDONÇA, KAIO CESAR CHABOLI ALEVI, NATHÁLIA PAIVA PEREIRA, ANNA CLAUDIA CAMPANER LIMA, JOÃO ARISTEU DA ROSA, MARIA TERCÍLIA VILELA DE AZEREDO OLIVEIRA D - 21 STUDY OF SPERMATOGENESIS OF TRIATOMA SHERLOCKI (HEMIPTERA, TRIATOMINAE) ANNA CLAUDIA CAMPANER LIMA, KAIO CESAR CHABOLI ALEVI, PRISCILA PASQÜETTO MENDONÇA, NATHÁLIA PAIVA PEREIRA, JOÃO ARISTEU DA ROSA, MARIA TERCÍLIA VILELA DE AZEREDO OLIVEIRA D - 22 NUCLEOLAR CYCLE IN TRIATOMA WILLIAMI (HEMIPTERA, TRIATOMINAE) NATHÁLIA PAIVA PEREIRA, PRISCILA PASQÜETTO MENDONÇA, KAIO CESAR CHABOLI ALEVI, ANNA CLAUDIA CAMPANER LIMA, JOÃO ARISTEU DA ROSA, MARIA TERCÍLIA VILELA DE AZEREDO OLIVEIRA 101 D - 23 QUANTITATIVE EVALUATION AND SPATIAL LOCALIZATION OF SPONTANEOUS AND MNU-INDUCED PROSTATE TUMORS IN GERBILS BIANCA FACCHIM GONÇALVES, SILVANA GISELE PEGORIN DE CAMPOS, CAMILA HELENA FACINA, LUIZ ROBERTO FALLEIROS JR, SEBASTIÃO ROBERTO TABOGA D - 24 MELATONIN: MITIGATING DAMAGES IN STREPTOZOTOCINDIABETIC RAT TESTIS CAROLINA FRANDSEN PEREIRA DA COSTA, MARINA GUIMARÃES GOBBO, MARIA ETELVINA PINTO, EDUARDO ALVES DE ALMEIDA, REJANE MAIRA GÓES D - 25 SERTOLI CELL INDEX IN THE FRUGIVOROUS BAT STURNIRA LILIUM DANIELLE BARBOSA MORAIS, SÉRGIO LUIS PINTO DA MATTA D - 26 SPERMATIC RESERVE IN THE TESTIS OF THE INSECTIVOROUS BAT MOLOSSUS MOLOSSUS DANIELLE BARBOSA MORAIS, SÉRGIO LUIS PINTO DA MATTA D - 27 EFFECTS OF THE INSECTICIDE ENDOSULFAN ON LEYDIG CELLS OF THE BIG FRUIT-EATING BAT ARTIBEUS LITURATUS (OLFERS,1818) DANIELLE BARBOSA MORAIS, ALESSANDRO BRINATI, SÉRGIO LUIS PINTO DA MATTA, MARIELLA BONTEMPO DUCA DE FREITAS, JULIANA SILVA ROCHA, SENDY MOREIRA REIS, JULIANA MATTOS SOUZA LIMA, MICHELE OLIVEIRA SANTOS D - 28 LONG-TERM SERTOLI CELL RESISTANCE IN TESTIS OF ADULT WISTAR RATS EXPOSED TO A SINGLE CADMIUM IP INJECTION. RODRIGO PAULA LEITE, MARY ANNE HEIDI DOLDER D - 29 ENVIRONMENTALLY REALISTIC HIGH DOSE OF CADMIUM INCREASES LIPID PEROXIDATION IN WISTAR RAT TESTIS: IS THE HEMATOTESTICULAR BARRIER A MAJOR TARGET OF FREE RADICALS? MARY ANNE HEIDI DOLDER, MARY ANNE HEIDI DOLDER, FERNANDA RAMOS GADELHA, LUÍS HENRIQUE GONZAGA RIBEIRO D - 30 TIME-DEPENDENT EFFECTS OF CADMIUM ON BLOOD VESSEL ENDOTHELIUM - HISTOPATHOLOGICAL AND STEREOLOGICAL ANALYSIS. RODRIGO PAULA LEITE, FERNANDA RAMOS GADELHA, MARY ANNE HEIDI DOLDER D - 41 GESTATIONAL EXPOSURE OF GERBILS TO ETHINYLESTRADIOL REDUCES THE NUMBER OF GONOCYTES AT BIRTH. MARIANA PULEGIO, ANA PAULA DA SILVA PEREZ, MARIA ETELVINA PINTO, REJANE MAIRA GÓES D - 42 MORPHOLOGICAL CHANGES IN THE PLACENTA OF ALLOXAN INDUCED DIABETIC RATS PRISCILLA SILVA FARIAS, KARINE DOS SANTOS SOUZA, ANDERSON CARLOS MARCAL, MARCIO ROBERTO VIANA DOS SANTOS, EMERSON TICONA FIORETTO, MARLÚCIA BASTOS AIRES D - 43 RELATION BETWEEN SPERM LENGTH AND SIZE OF THE INDIVIDUAL WHO PRODUCES GLENDA DIAS, HELEN PINTO SANTOS, JOSÉ LINO-NETO D - 44 GERM CELLS DEVELOPMENT IN THE DOURADO SALMINUS FRANCISCANUS LIMA & BRITSKI, 2007 FROM THE SÃO FRANCISCO RIVER BASIN: A HISTOLOGICAL AND ULTRASTRUCTURAL STUDY LEONARDO JOSÉ ALVES DE FREITAS, PAULA SUZANNA PRADO, KLEBER B. SANTIAGO, MARCOS VINICIUS TELES GOMES, NILO BAZZOLI, ELIZETE RIZZO D - 45 BISPHENOL A (BPA) AND INDOLE-3-CARBINOL (I3C): EFFECTS ON THE MALE RATS PROSTATE DEVELOPMENT LÍVIA TERESA RIBEIRO DA SILVEIRA, JOYCE ZALOTTI BRANDT, TONY FERNANDO GRASSI, WAGNER JOSÉ FAVARO, RAQUEL FANTIN DOMENICONI, PATRÍCIA FERNANDA FELIPE PINHEIRO, LUIS FERNANDO BARBISAN, WELLERSON RODRIGO SCARANO D - 46 REPRODUCTION AND SPAWNING OF DOURADO SALMINUS FRANCISCANUS LIMA & BRITSKI, 2007 IN THE SÃO FRANCISCO RIVER, TRÊS MARIAS, MINAS GERAIS, BRAZIL LEONARDO JOSÉ ALVES DE FREITAS, PAULA SUZANNA PRADO, KLEBER B. SANTIAGO, MARCOS VINICIUS TELES GOMES, NILO BAZZOLI, ELIZETE RIZZO D - 47 EXPRESSION OF THE ESTROGEN RECEPTOR ERBETA IN THE VENTRAL AND DORSAL PROSTATE OF AGING RATS MÔNICA MORAIS SANTOS, RACHEL PIRES REIS, AMANDA CRISTINA REIS GONZAGA, ANDRÉ GUSTAVO OLIVEIRA, GERMÁN ARTURO BOHORQUEZ MAHECHA, MARIA CHRISTINA AVELLAR, CLEIDA APARECIDA DE OLIVEIRA D - 31 SPERMATOGENIC EFFICIENCY OF WILD RODENT OLIGORYZOMYS NIGRIPES (RODENTIA, MURIDAE) MAYTÊ KOCH BALARINI, ANA CAROLINA TORRE MORAIS, TATIANA PRATA DE MENEZES, DANIELLE BARBOSA MORAIS, MICHELE OLIVEIRA SANTOS, FAUSTO FERRAZ, SÉRGIO LUIS PINTO DA MATTA D - 48 EFFECTS OF THE HERBICIDE ATRAZINE IN THE EXPRESSION OF P450-AROMATASE IN THE TESTIS, EPIDIDYMIS AND PROSTATE OF ADULT MALE RATS ELISÂNGELA MARTINS DOS SANTOS, CRISTIANO GUIMARÃES PIMENTA, POLLYANA RABELO NUNES CAMPOS, GERMÁN ARTURO BOHORQUEZ MAHECHA, CLEIDA APARECIDA OLIVEIRA D - 32 COMPARATIVE STUDY OF APOPTOTIC PROFILE ACTIVATED BY INTRINSIC AND EXTRINSIC PATHWAYS IN VENTRAL PROSTATE OF AGING RATS AMANDA CRISTINA REIS GONZAGA, MÔNICA MORAIS SANTOS, JÚNIA DAYRELL DE MOURA CORDEIRO, GERMÁN ARTURO BOHÓRQUEZ MAHECHA, CLEIDA APARECIDA OLIVEIRA D - 49 REPRODUCTIVE AND HISTOLOGICAL PARAMETERS OF ADULT RATS EXPOSED TO SIBUTRAMINE GABRIELA MISSASSI, CIBELE DOS SANTOS BORGES, RAQUEL FRENEDOSO DA SILVA, JULIANA ELAINE PEROBELLI, MARCIANA SANABRIA, THAIS PETROCHELLI BANZATO, MARIANA MACÊDO DE OLIVEIRA, WILMA DE GRAVA KEMPINAS D - 33 ACTIONS OF AÇAÍ PULP (EUTERPE EDULLIS) ON THE EPIDIDYMAL CAPUT REGION HISTOMORPHOMETRY OF WISTAR RATS EXPOSED TO CADMIUM CHLORIDE (CDCL2) ANA CLÁUDIA FERREIRA SOUZA, GRAZIELA DOMINGUES DE ALMEIDA LIMA, TATIANA PRATA MENEZES, MARLI DO CARMO CUPERTINO, SÉRGIO LUÍS PINTO DA MATTA, MARIANA MACHADO NEVES D - 50 EVALUATION OF REPRODUCTIVE AND HISTOLOGICAL PARAMETERS ON TESTIS AND EPIDIDYMIS OF ADULT MALE RATS EXPOSED TO SIMVASTATIN. THAIS PETROCHELLI BANZATO, MARIANA MACÊDO OLIVEIRA, JULIANA ELAINE PEROBELLI, MARCIANA SANABRIA, CIBELE DOS SANTOS BORGES, RAQUEL FRENEDOSO DA SILVA, GABRIELA MISSASSI, WILMA DE GRAVA KEMPINAS D - 34 EFFECTS OF AÇAÍ PULP (EUTERPE EDULLIS) ON CADMIUM CHLORIDE-INDUCED DAMAGE IN EPIDIDYMAL CAPUT REGION OF ADULT RATS: A MORPHOMETRIC STUDY GRAZIELA DOMINGUES DE ALMEIDA LIMA, TATIANA PRATA MENEZES, VIVIANE SILVEIRA GORETE MOURO, RAFAEL REIS DOMINGUES, NAYARA MAGALHÃES GONÇALVES, MARLI DO CARMO CUPERTINO, SÉRGIO LUÍS PINTO DA MATTA SERGIO, MARIANA MACHADO NEVES D - 51 NUCLEUS OF PROSTATIC EPITHELIAL CELL OF SENESCENT GERBILS AS A TARGET ORGANELLE OF STUDY AFTER HORMONE DEPRIVATION SILVANA GISELE PEGORIN DE CAMPOS, BIANCA FACCHIM GONÇALVES, WELLERSON RODRIGO SCARANO, LUIZ ROBERTO FALLEIROS JÚNIOR, SEBASTIÃO ROBERTO TABOGA D - 35 NO LEVEL AS AN INDICATOR OF SICKNESS BEHAVIOR AND UTERINE MORPHOPHYSIOLOGICAL CHANGES IN LPS-TREATED PREGNANT MICE BRUNO ZAVAN, ELIANA MARA OLIVEIRA LIPPE, ALEXANDRE GIUSTI-PAIVA, AUREO TATSUMI YAMADA, VALDEMAR ANTONIO PAFFARO JUNIOR D - 36 EFFECTS OF THE MUTATION IN THE FOXN1 GENE IN THE ADULT MICE TESTIS STRUCTURE AND FUNCTION CAROLINA FELIPE ALVES DE OLIVEIRA, GLEIDE FERNANDES AVELAR, LUIZ RENATO DE FRANÇA D - 37 PROTEIN UNDERNUTRITION MODIFIES THE EPITHELIUMMESENCHYME INTERACTION AND DELAYS THE PROSTATE DIFFERENTIATION IN NEONATAL RATS CRISTIANE FIGUEIREDO PINHO, PATRÍCIA FERNANDA FELIPE PINHEIRO, RAQUEL FANTIN DOMENICONI, BRUNO CÉSAR SCHIMMING, WAGNER JOSÉ FAVARO, SÉRGIO PEREIRA, SILVANA GISELE PEGORIN DE CAMPOS, SEBASTIÃO ROBERTO TABOGA, PATRÍCIA ALINE BOER, WELLERSON RODRIGO SCARANO D - 38 STROMAL CHANGES IN THE VENTRAL PROSTATE OF LONG- TERM OBESE RATS ARE ASSOCIATED TO INCREASED MMP-9 ACTIVITY AND HIGH VEGF CONTENT SILAS AMÂNCIO SILVA, RENATO SIMÕES CORDEIRO, TATIANA CARLA TOMIOSSO, SEBASTIÃO ROBERTO TABOGA, REJANE MAIRA GÓES, DANIELE LISBOA RIBEIRO D - 52 EXTRAORDINARY DIVERSITY OF SPERM MORPHOLOGY AMONG MARINE BIVALVES OF THE SUPERFAMILY TELLINOIDEA (MOLLUSCA): SPERMATOZOA RANGING FROM ECT-AQUASPERM TO THOSE WITH SCREW-LIKE HEAD, AND UNCOMMON PARASPERMATIC FEATURES GISELE ORLANDI INTROÍNI, LENITA DE FREITAS TALLARICO, FLÁVIO DIAS PASSOS, SUGURU UJINO, SHIRLEI MARIA RECCO-PIMENTEL D - 53 HIGH-FAT DIET ACT AS A PROMOTIONAL AGENT ON PROSTATE CARCINOGENESIS OF GERBILS. CAMILA HELENA FACINA, BIANCA FACCHIM GONÇALVES, LUIZ ROBERTO FALLEIROS JR, SEBASTIÃO ROBERTO TABOGA D - 54 EFFECT OF EXTRACTS FROM THE SEED OF NEEM(AZADIRACHTA INDICA A JUSS) ON THE MORPHOMETRY SEMINIFEROUS TUBULES OF WISTAR RATS. FERNANDA CAROLINA RIBEIRO DIAS, ALLUANAN ADELSON NASCIMENTO, JESSICA SANTANA, OLAVIO CAMPOS JUNIOR, SIMONE CABRAL, WOLFGANG HARAND, ELIZABETH NEVES DE MELO D - 55 ADMINISTRATION OF AN ANDROGEN COMPOUND TO FEMALE WISTAR RATS DURING GESTATIONAL AND LACTATIONAL PERIODS AND THE ASSESSMENT OF THE EFFECTS ON THE UTERUS OF THE FEMALE OFFSPRING MARINA TREVIZAN GUERRA, RAQUEL FRENEDOSO DA SILVA, MARCIANA SANABRIA, GAIL GROSSMAN, PETER PETRUSZ, WILMA DE GRAVA KEMPINAS D - 39 THE SIZE OF THE SPERM FOLLOWS THE SIZE OF THE INDIVIDUALS WHO PRODUCE IN CLOSELY RELATED SPECIES? HELEN CRISTINA PINTO SANTOS, GLENDA DIAS, RAQUEL APARECIDA COSTA, JOSÉ LINO NETO D - 56 EXPRESSION OF GLYCOCONJUGATES IN THE UTERINE NATURAL KILLER CELLS FROM GENETICALLY MODIFIED MOUSE. ÉVILA DA SILVA LOPES SALLES, PAULO FERNANDO DE SOUZA JÚNIOR, ANDRÉA MOLLICA DO AMARANTE PAFFARO, BARBARA ANNE CROY, AÚREO TATSUMI YAMADA, VALDEMAR ANTÔNIO PAFFARO JÚNIOR D - 40 VEGF PROTEIN EXPRESSION IN THE PLACENTA OF ALLOXAN INDUCED DIABETIC RATS KARINE DOS SANTOS SOUZA, PRISCILLA SILVA FARIAS, WALDECY DE LUCCA JUNIOR, ANDERSON CARLOS MARCAL, MARCIO ROBERTO VIANA DOS SANTOS, THIAGO ALVES BRAGA, EMERSON TICONA FIORETTO, MARLÚCIA BASTOS AIRES D - 57 OXIDATIVE STRESS IN THE KIDNEY OF FEMALE RATS WITH OR WITHOUT REPRODUCTIVE ACTIVITY DURING AGING JORDANA SALETE PUTTI, ANA CAROLINA ALMEIDA DA SILVA, TIAGO BOEIRA SALOMON, CAMILE SAUL BEHLING 102 D - 58 VITELLOGENIN AND ZONA RADIATA PROTEINS IMMUNODETECTION IN LIVER OF LAMBARI ASTYANAX FASCIATUS FROM THE FURNAS RESERVOIR, GRANDE RIVER, BRAZIL PAULA SUZANNA PRADO, ANA PAULA BARBOSA PINHEIRO, ELIZETE RIZZO D - 59 LIVER IGF-I E IGF-II IMMUNOCHEMICAL QUANTIFICATION IN ASTYANAX FASCIATUS FROM ESTROGEN CONTAMINATED AREAS FROM THE FURNAS RESERVOIR, GRANDE RIVER, BRAZIL PAULA SUZANNA PRADO, ANA PAULA BARBOSA PINHEIRO, ELIZETE RIZZO NOGUEIRA, GERMÁN ARTURO BOHORQUEZ MAHECHA, CLEIDA APARECIDA DE OLIVEIRA D - 76 INVESTIGATION OF AQUAPORIN-9 IN THE EFFERENT DUCTULES AND EPIDIDYMIS OF BIG FRUIT-EATING BAT ARTIBEUS LITURATUS DURING THE ANNUAL REPRODUCTIVE CYCLE REGIANA LUCIA DE OLIVEIRA, JOSÉ CARLOS NOGUEIRA, GERMÁN ARTURO BOHORQUEZ MAHECHA, CLEIDA APARECIDA DE OLIVEIRA D - 60 FECUNDITY AND VITELLOGENIC OOCYTE DIAMETER ASSESSMENT OF LAMBARI ASTYANAX FASCIATUS INHABITING EDCS CONTAMINATED WATERS IN FURNAS RESERVOIR, GRANDE RIVER, BRAZIL ANA PAULA BARBOSA PINHEIRO, PAULA SUZANNA PRADO, JÉSSICA FIGUEIREDO ABREU, ELIZETE RIZZO D - 77 EFFECT OF NEEM OIL (AZADIRACHTA INDICA A. JUSS) ON MORPHOLOGY OF THE TESTIS OF CERAEOCHRYSA CLAVERI (NAVÁS, 1911) (NEUROPTERA: CHRYSOPIDAE) ANA SILVIA GIMENES GARCIA, ELTON LUIZ SCUDELER, MONIQUE CAMPOS PEREIRA, PATRICIA FERNANDA FELIPE PINHEIRO, DANIELA CARVALHO DOS SANTOS D - 61 STRUCTURAL AND ULTRASTRUCTURAL ANALYSES OF OOCYTES OF THE DANIO RERIO EXPOSED TO GLYPHOSATE NEIDE ARMILIATO, EVELISE MARIA NAZARI, DIB AMMAR, YARA MARIA RAUH MÜLLER D - 78 THE OOGENESIS PROCESS DURING THE REPRODUCTIVE CYCLE OF LOXOSCELES INTERMEDIA (ARANEAE: SICARIIDAE). EVERTON FOGAÇA, CLAUDIA FEIJÓ ORTOLANI-MACHADO D - 62 MORPHOLOGICAL AND CYTOCHEMICAL STUDIES OF PREGNANT MOUSE UTERUS FROM NORMAL AND GENETIC MODIFIED MOUSE AFTER EMBRYONIC LESION RAQUEL MEZZALIRA RUANO, ÉVILA DA SILVA LOPES SALLES, ANDREA MOLLICA DO AMARANTE PAFFARO, BARBARA ANNE CROY, ÁUREO TATISUMI YAMADA, VALDEMAR ANTONIO PAFFARO JUNIOR D - 79 ASSESSMENT OF SPERMATOGENESIS AND DIAMETER OF SEMINIFEROUS TUBULES IN THE LAMBARI ASTYANAX FASCIATUS FROM CONTAMINATED SITES IN FURNAS RESERVOIR, GRANDE RIVER, BRAZIL ANA PAULA BARBOSA PINHEIRO, PAULA SUZANNA PRADO, FABRÍCIO FLÁVIO THEOPHILO DOMINGOS, ELIZETE RIZZO D - 63 PLACENTAL INDOLEAMINE 2,3-DIOXYGENASE (IDO) ACTIVITY IN RENAL-TRANSPLANTED PREGNANT WOMEN KAREN MATIAS DO PRADO, SIMONE CORREA DA SILVA, LEANDRO GUSTAVO OLIVEIRA, SILVANA SANDRI, LARISSA DE SÁ LIMA, MELISSA CAVALHEIRO TOURINO, ANA CAMPA, CRISTOFORO SCAVONE, NIELS OLSEN SARAIVA CAMARA, ESTELA BEVILACQUA D - 80 SPERMATOGENIC CYCLE LENGTH AND SPERM PRODUCTION IN A FRESHWATER TURTLE KINOSTERNONSCORPIOIDES ALANA LISLEA DE SOUSA, PAULO HENRIQUE ALMEIDA CAMPOS- JUNIOR, GUILHERME MATTOS JARDIM COSTA, LUIZ RENATO DE FRANÇA D - 64 SPERM MORPHOLOGY OF RHYZOPERTHA DOMINICA (COLEOPTERA: BOSTRICHIDAE) GLENDA DIAS, HELEN PINTO SANTOS, JOSÉ LINONETO D - 65 IMMUNOLOCALIZATION OF HOMOGALACTURONAN PECTINS, ARABINOGALACTAN PROTEINS AND HEMICELLULOSES IN THE FILIFORM APPARATUS OF PITCAIRNIA ENCHOLIRIOIDES L.B.SM. (BROMELIACEAE) SIMONE PETRUCCI MENDES, ALEXANDRA A. MASTROBERTI, JORGE E. A. MARIATH, RICARDO C. VIEIRA, KAREN L. G. DE TONI D - 66 POLYMORPHISM OF SPERMATOZOA IN THE INSECT TRYPOXYLON (HYMENOPTERA: CRABRONIDAE) LUIZ FERNANDO GOMES, UYRÁ ZAMA, HELEN PINTO SANTOS, JOSÉ LINO-NETO D - 67 AQUAPORIN-9 LOCALIZATION IN THE GERBIL EPIDIDYMIS DURING POSTNATAL DEVELOPMENT CARLA DE MORAES MACHADO, WELLERSON RODRIGO SCARANO, PATRICIA FERNANDA FELIPE PINHEIRO, WAGNER JOSÉ FÁVARO, RAQUEL FANTIN DOMENICONI D - 68 SPERMATOGONIAL STEM CELL NICHE IN THE SEXUALLY MATURE BULLFROG (LITHOBATES CATESBEIANUS) LUIZ HENRIQUE DE CASTRO ASSIS, FERNANDA VIEIRA DA SILVA CRUZ, TÂNIA MARA SEGATELLI, LUIZ RENATO DE FRANÇA D - 69 MATERNAL OBESITY AND POSTNATAL OVERNUTRITION IMPAIRS LEYDIG CELLS FUNCTION IN ADULT RATS MARIA ETELVINA PINTO, THIAGO FERES PISSOLATO, DANIELE LISBOA RIBEIRO, REJANE MAIRA GÓES D - 70 DURATION OF SPERMATOGENESIS IN THE SPINY-RAT, PROECHIMYS GUYANNENSIS (RODENTIA: ECHIMYIDAE) NATHÁLIA DE LIMA E MARTINS LARA, IVAN CARLOS DOS SANTOS, GUILHERME MATTOS JARDIM COSTA, PAULO HENRIQUE ALMEIDA CAMPOS-JUNIOR, ANA PAULA MADUREIRA, MARCOS SANTOS ZANINI, LUIZ RENATO DE FRANÇA D - 71 FECUNDITY, OOCYTE DIAMETER, GONADAL MATURATION AND GONADOSSOMATIC INDEX OF THE CURIMBATÁ PROCHILODUS LINEATUS IN THREE SECTIONS OF THE GRANDE RIVER BASIN, DOWNSTREAM FROM THE PORTO COLOMBIA DAM. VIOLETA DA ROCHA PERINI, CLÁUDIA KELLY FERNANDES CRUZ, NILO BAZZOLI, ELIZETE RIZZO D - 72 COLLARED PECCARY (TAYASSU TAJACU) SPERMATOGENESIS PROGRESSION AND DE NOVO TESTIS MORPHOGENESIS FROM TESTIS TISSUE AND CELL SUSPENSION ECTOPICALLY XENOGRAFTED IN IMMUNODEFICIENT MICE PAULO HENRIQUE DE ALMEIDA CAMPOS JUNIOR, G. M. J. COSTA, S. M. S. N. LACERDA, G. F. AVELAR, D. A. A. GUIMARÃES, P. R. KAHWAGE, L. R. FRANÇA D - 73 SELF-RENEWAL AND EXPANSION OF NILE-TILAPIA (OREOCHROMIS NILOTICUS) SPERMATOGONIAL STEM CELLS IN CULTURE SAMYRA MARIA DOS SANTOS NASSIF LACERDA, MARIANA DE ARAÚJO DA SILVA, GUILHERME MATTOS JARDIM COSTA, PAULO HENRIQUE ALMEIDA CAMPOS-JÚNIOR, TÂNIA MARA SEGATELLI, LUIZ RENATO DE FRANÇA D - 74 DEVELOPMENT OF A CRYOPRESERVATION PROTOCOL FOR SPERMATOGONIAL STEM CELL IN HORSES GUILHERME MATTOS JARDIM COSTA, G. F. AVELAR, J. V. REZENDE-NETO, S. M. S. N. LACERDA, P. H. A. CAMPOS- JÚNIOR, F. G. P. MARTINS, L. R. FRANÇA D - 75 DISTRIBUTION OF ESTROGEN RECEPTORS ERALPHA AND ERBETA IN THE PROSTATE AND AMPULLARY GLANDS OF BIG FRUIT-EATING BAT ARTIBEUS LITURATUS DURING THE ANNUAL REPRODUCTIVE CYCLE. GABRIEL HENRIQUE CAMPOLINA SILVA, REGIANA LUCIA DE OLIVEIRA, JOSÉ CARLOS D - 81 EXTENUATING EXERCISE INCREASES THE UNK CELLS NUMBER AND DECREASES EMBRYO VIABILITY IN PREGNANT MOUSE YAN TEIXEIRA FELBER, KAMILA LEITE RODRIGUES, CAMILA A BRAGA, ANDREA MOLLICA AMARANTE PAFFARO, VALDEMAR ANTONIO PAFFARO JUNIOR D - 82 TYPE A SPERMATOGONIA DISTRIBUTION IN THE TESTIS OF SEXUALLY MATURE NILE-TILAPIA (OREOCHROMIS NILOTICUS) PEDRO MANUEL APONTE GARCIA, TÂNIA MARA SEGATELLI, LUCAS SANTOS E SOUZA, LUIZ RENATO DE FRANÇA D - 83 CIRCADIAN PROTEINS CLOCK AND BMAL1 IN THE CHROMATOID BODY, A RNA GRANULE OF MALE GERM CELLS RITA LUIZA PERUQUETTI, PAOLO SASSONE-CORSI D - 84 ETHNOMEDICINES USED IN ALFENAS (BRAZIL) FOR FEMALE REPRODUCTIVE DISORDERS JULIANE DE LIMA PASSOS, FERNANDO FELICIONI, ANA FLÁVIA GONTIJO PIMENTA, GIOVANA BARBARINE LONGATO, VALDEMAR ANTÔNIO PAFFARO JÚNIOR, ANDRÉA MOLLICA DO AMARANTE PAFFARO D - 85 EFFECTS OF THE FUNGICIDE TEBUCONAZOLE ON LEYDIG CELLS FROM THE FRUGIVOROUS BATS ARTIBEUS LITURATUS (OLFERS, 1818) DIANE DA CRUZ MIRANDA, ÉRICA RITA DOS SANTOS LEITE, TÚLIO FIORINI CARVALHO, RAQUEL ARMINDA CARVALHO MACHADO, DANIELA VALENTE DE ANDRADE, MIRLAINE SOARES BARROS, ALESSANDRO BRINATI, MARIANA MACHADO NEVES, MARIELLA BOMTEMPO DUCA DE FREITAS, SÉRGIO LUIS PINTO DA MATTA D - 86 KARYOTYPE OF TRIATOMA MELANOCEPHALA (HEMIPTERA: REDUVIIDAE) CAMILA HELENA FACINA, KAIO CESAR CHABOLI ALEVI, MARIA TERCÍLIA VILELA DE AZEREDO OLIVEIRA D - 87 ARTEMISININ INCREASES UTERINE NATURAL KILLER CELL NUMBER AND CAUSES EMBRYO LOSS DURING MOUSE PREGNANCY FERNANDO FELICIONI, KAMILA LEITE RODRIGUES, VALDEMAR ANTÔNIO PAFFARO JÚNIOR, ANDRÉA MOLLICA DO AMARANTE PAFFARO D - 88 ANALYSIS OF VIMENTIN EXPRESSION IN MICE CHORIOALLANTOIC PLACENTAL TROPHOBLAST GIANT CELLS PEDRO LUIZ ANDRADE SCHERHOLZ, DIVA DENELLE SPADACCI-MORENA, SIMA GODOSEVICIUS KATZ D - 89 ACUTE EFFECT OF THE FUNGICIDE MANCOZEB ON LEYDIG CELLS FROM THE FRUGIVOROUS BATS ARTIBEUS LITURATUS (OLFERS, 1818) DIANE DA CRUZ MIRANDA, ÉRICA RITA DOS SANTOS LEITE, TÚLIO FIORINI CARVALHO, RAQUEL ARMINDA CARVALHO MACHADO, DANIELA VALENTE DE ANDRADE, MIRLAINE SOARES BARROS, ALESSANDRO BRINATI, MARIANA MACHADO NEVES, MARIELLA BONTEMPO DUCA DE FREITAS, SÉRGIO LUIS PINTO DA MATTA D - 90 ANNUAL REPRODUCTIVE CYCLE OF MALES OF THE FLAT-FACED FRUIT-EATING BAT, ARTIBEUS PLANIROSTRIS (CHIROPTERA: PHYLLOSTOMIDAE) MATEUS RODRIGUES BEGUELINI, CINTIA CRISTINA ISICAWA PUGA, SEBASTIÃO ROBERTO TABOGA, ELIANA MORIELLE VERSUTE D - 91 SENESCENCE AND PROSTATE: FIBROBLASTIC GROWTH FACTORS, ANDROGEN AND PROLACTIN INTERACTIONS. AMANDA CIA HETZL, FABIO MONTICO, LARISSA AKEMI KIDO, RAISA MISTIERI LORENCINI, EDUARDO MARCELO CÂNDIDO, VALÉRIA HELENA ALVES CAGNON D - 92 BIOCHEMICAL CHARACTERIZATION OF THE NUCLEAR ANNULUS OF BULL SPERM AND ITS ASSOCIATION WITH CHROMATIN MOLINE SEVERINO LEMOS, PRISCILA FERREIRA MOREIRA, ALBERTO DA SILVA MORAES, FÁBIO DE OLIVEIRA, ROMUALDO MORANDI FILHO, MARCELO EMÍLIO BELETTI D - 93 CYTOTOXIC EFFECTS OF SODIUM ARSENITE ON MICE SPERM CELLS MARIA DE LOURDES GOMES PEREIRA, FERNANDO GARCIA E COSTA 103 D - 94 REPRODUCTIVE PARAMETERS OF DIABETIC RATS TREATED WITH BRAZIL NUT EXTRACT OR SODIUM SELENITE LEONARDO PARREIRA SILVA NASCIMENTO, VANESSA CARDOSO PIRES, DANIEL ARAKI RIBEIRO, ANDREA PITTELLI BOIAGO GOLLUCKE, LUIS FILIPE DE OLIVEIRA FIGLIOLINO, HIROCHI YAMAMURA, NATÁLIA MANZATTI MACHADO ALENCAR, ODAIR AGUIAR JUNIOR D - 111 MATERNAL BEHAVIOR IN PREGNANCY AFTER TREATMENT WITH HYDROALCOHOLIC EXTRACT OF CASSIA ANGUSTIFOLIA DURING EMBRYO IMPLANTATION PERIOD RAFAELA SILVA DOS SANTOS, BRUNO ZAVAN, VALDEMAR ANTÔNIO PAFFARO JÚNIOR, ANDRÉA MOLLICA DO AMARANTE PAFFARO D - 95 AGING ALTERATIONS IN TESTIS AND EPIDIDYMIS: COULD HETEROPTERYS TOMENTOSA IMPROVE THESE CHANGES? FABRICIA DE SOUZA PREDES, MARIA APARECIDA DA SILVA DIAMANTE, JULIANA CASTRO MONTEIRO, HEIDI DOLDER D - 112 HISTOLOGICAL DESCRIPTION OF THE OOGENESIS OF HYPOSTOMUS FRANCISCI (LÜTKEN, 1874) (SILURIFORMES, LORICARIIDAE) CAPTURED IN THE ITAPECERICA RIVER, DIVINÓPOLIS, MG, BRAZIL. CAMILA FERREIRA SALES, REGIANNE FERREIRA SILVA, MARILIA GABRIELA C AMARAL, ROSY I. MACIEL AZAMBUJA RIBEIRO, FABRICIO FLÁVIO THEOPHILO DOMINGOS, RALPH GRUPPI THOMÉ, HÉLIO BATISTA DOS SANTOS D - 96 PROSTATIC STROMA FEATURES IN THE SENESCENCE AND FOLLOWING ANTI-ANGIOGENIC THERAPY X STROMAL REACTIVITY DURING CANCER PROGRESSION IN THE TRANSGENIC ADENOCARCINOMA OF MOUSE PROSTATE (TRAMP) MODEL FABIO MONTICO, AMANDA CIA HETZL, EDUARDO MARCELO CÂNDIDO, LARISSA AKEMI KIDO, RAÍSA MISTIERI LORENCINI, VALÉRIA HELENA ALVES CAGNON D - 113 STUDY OF SPERMATOGENESIS OF TRIATOMA JUAZEIRENSIS (HEMIPTERA, REDUVIIDAE) KAIO CESAR CHABOLI ALEVI, PRISCILA PASQÜETTO MENDONÇA, NATHÁLIA PAIVA PEREIRA, NAYARA FERNANDA DA COSTA CASTRO, LARISSA CENTURION GANDOLPHI, MARIA TERCÍLIA VILELA DE AZEREDO OLIVEIRA D - 97 DI-N-BUTYL-PHTHALATE (DBP) EFFECTS ON THE PROSTATE OF ADULT RATS EXPOSED FROM THE FETAL PERIOD AND INITIATED BY MNU: BIOMETRICAL, HORMONAL AND STEREOLOGICAL PARAMETERS TALITA MELLO SANTOS, ANDRÉ REBELLO PEIXOTO, JOYCE ZALOTTI BRANDT, LEONARDO DE OLIVEIRA MENDES, JOSÉ EDUARDO BOZANO, WAGNER JOSÉ FAVARO, RAQUEL FANTIN DOMENICONI, JANETE A. ANSELMO-FRANCI, WELLERSON RODRIGO SCARANO D - 98 ANTIANGIOGENIC THERAPIES AND MOLECULAR RESPONSE OF THE VENTRAL PROSTATE MICROENVIRONMENT IN ELDERLY MICE. LARISSA AKEMI KIDO, AMANDA CIA HETZL, EDUARDO MARCELO CÂNDIDO, FABIO MONTICO, RAÍSA MISTIERI LORENCINI, VALÉRIA HELENA ALVES CAGNON D - 99 INCIDENCE OF LESIONS AND MAPK/AKT PATHWAY EVALUATION IN THE ADULT RATS PROSTATE EXPOSED FROM THE FETAL PERIOD TO DI-NBUTYL-PHTHALATE (DBP) ANDRÉ REBELO PEIXOTO, TALITA DE MELLO SANTOS, WAGNER JOSÉ FAVARO, PATRÍCIA FERNANDA F. PINHEIRO, RAQUEL FANTIN DOMENICONI, SILVANA GISELE PEGORIN DE CAMPOS, SÉRGIO LUIS FELISBINO, SEBASTIÃO ROBERTO TABOGA, WELLERSON RODRIGO SCARANO D - 100 CHARACTERISTICS OF INTERACTION BETWEEN ESTROGEN AND PROGESTERONE ON THE GERBIL PROSTATE RICARDO ALEXANDRE FOCHI, JULIA QUILLES ANTONIASSI, LUIZ ROBERTO FALLEIROS JÚNIOR, SEBASTIÃO ROBERTO TABOGA D - 101 INFUSION AND EXTRACT OF ARTEMISIA VULGARIS IMPAIR MOUSE PREGNANCY FERNANDO FELICIONI, PAULO SÉRGIO DE SOUZA PRIZMIC KIMAR, VALDEMAR ANTÔNIO PAFFARO JÚNIOR, ANDRÉA MOLLICA DO AMARANTE PAFFARO D - 102 EFFECT OF PUNICA GRANATUM HIDROALCOHOLIC EXTRACT IN MICE PAULO FERNANDO DE SOUZA JR, DANIELE ABUD QUAGLIANO, CAMILA MIRANDA PERNAMBUCO, ALEXANDRE GIUST PAIVA, ANDREA MOLLICA DO AMARANTE PAFFARO D - 103 CONTRACEPTIVE POTENTIAL OF SUBCHRONIC ETHANOLIC EXTRACT OF MAYTENUS ILICIFOLIA MART. EX REISSEK. IN FEMALE WISTAR RATS SILVANE SOUZA ROMAN, CARLA GIANE LOSS, ASSIS ECKER, BRUNA CLAUDIA COPPE, TAÍS REGINA FIORENTIN, ARNO ERNESTO HOFMANN JUNIOR D - 104 MORPHOLOGICAL ASPECTS OF THE INITIAL STAGES OF TOXOPLASMA GONDII INVASION IN THE HUMAN CHORIONIC VILLI SARA HISSAE HIRAIWA, LETÍCIA DE SOUZA CASTRO FILICE, BRENO COSTA LANDIM, PRISCILA SILVA FRANCO, ELOISA AMÁLIA VIEIRA FERRO, JULIANA GONZAGA DE OLIVEIRA D - 105 ANTIANGIOGENIC THERAPY AND STEM CELL REACTIVITY OF THE TRANSGENIC ADENOCARCINOMA OF MOUSE DORSOLATERAL PROSTATE (TRAMP) MODEL VALÉRIA HELENA ALVES CAGNON, FABIO MONTICO, AMANDA CIA HETZL, RAÍSA MISTIERI LORENCINI, LARISSA AKEMI KIDO, WAGNER JOSÉ FÁVARO, MARCUS A. F. CORAT, DELMA P. ALVES, LUIZ A.C. PASSOS D - 106 EFFECT OF SYZYGIUM CUMINI HIDROALCOHOLIC EXTRACT IN MICE PREGNANCY RODOLFO CABRAL MARCELINO, WESLEY FERNANDES FONSECA D - 107 DETERMINATION OF (AG ↓ CT) SEQUENCES IN T. TIBIAMACULATA THROUGH RESTRICTION ENDONUCLEASE ALUI NAYARA FERNANDA DA COSTA CASTRO, ADAUTO DE OLIVEIRA BORGUETI, ROSANA SILISTINO-SOUZA, LARISSA CENTURION GANDOLPHI, MARIA TERCÍLIA VILELA DE AZEREDO-OLIVEIRA D - 114 EFFECT OF INFUSION OF RUTA GRAVEOLENS IN THE PREIMPLANTATIONAL AND IMPLANTATIONAL PERIOD OF MICE PREGNANCY CAMILA ALVARES BRAGA, WESLEY FERNANDES FONSECA, VALDEMAR ANTÔNIO PAFFARO JÚNIOR, ANDRÉA MOLLICA DO AMARANTE PAFFARO D - 115 DESCRIPTION OF SOMATIC AND GERM CELLS IN TESTIS OF ARMORED CATFISH HYPOSTOMUS FRANCISCI (LÜTKEN, 1874) COLLECTED IN THE PARAOPEBA RIVER, SÃO FRANCISCO RIVER BASIN, BRAZIL NATÁLIA RIBEIRO ALVES, CAMILA FERREIRA SALES, FABRÍCIO FLÁVIO THEOPHILO DOMINGOS, RALPH GRUPPI THOMÉ, FÁBIO PEREIRA ARANTES, YOSHIMI SATO, HÉLIO BATISTA DOS SANTOS D - 116 FECUNDITY AND OOCYTE DIAMETER OF THREE SPECIES FROM THE GRANDE RIVER BASIN, DOWNSTREAM FROM THE PORTO COLOMBIA DAM: AN COMPARATIVE ANALYSIS IN TWO SITES CLÁUDIA KELLY FERNANDES DA CRUZ, VIOLETA DA ROCHA PERINI, ALESSANDRO PASCHOALINI LOUREIRO, NILO BAZZOLI, ELIZETE RIZZO D - 117 SPERMIOGENESIS ANALYSIS IN TWO CRYPTIC SPECIES OF TRIATOMINES IN BRASILIENSIS SUBCOMPLEX KAIO CESAR CHABOLI ALEVI, PRISCILA PASQÜETTO MENDONÇA, NATHÁLIA PAIVA PEREIRA, BRUNNO BOTELHO BORGES, ANNA CLAUDIA CAMPANER LIMA, MARIA TERCÍLIA VILELA DE AZEREDO OLIVEIRA D - 118 HETEROCHROMATIN PATTERN IN HOLOCENTRIC CHROMOSOMES OF TRIATOMA LENTI AND T. SHERLOCKI (HEMIPTERA: REDUVIIDAE) KAIO CESAR CHABOLI ALEVI, PRISCILA PASQÜETTO MENDONÇA, NATHÁLIA PAIVA PEREIRA, BRUNNO BOTELHO BORGES, ANNA CLAUDIA CAMPANER LIMA, MARIA TERCÍLIA VILELA DE AZEREDO OLIVEIRA D - 119 EFFECTS OF EXPERIMENTAL DIABETES IN INTERSTITIAL TISSUE AND LEYDIG CELLS OF ADULT WISTAR RATS ALLUANAN ADELSON DO NASCIMENTO SILVA, JESSICA SANTANA DE OLIVEIRA, OLÁVIO CAMPOS JÚNIOR, FERNANDA CAROLINA RIBEIRO DIAS, RODRIGO RIBEIRO DE OLIVEIRA, SÍLVIA REGINA ARRUDA DE MORAES, ELIZABETH NEVES DE MELO D - 120 STROMAL CELL DERIVED FACTOR-2 (SDF-2) AT MATERNAL-FETAL INTERFACE ALINE RODRIGUES LORENZON, SHAKER CHUCK FARAH, SUSAN J FISHER, ESTELA BEVILACQUA D - 121 CARUNCULES AND BOVINE ANTIGEN LEUKOCYTE (BOLA) DURING PREGNANCY MARCELO EMÍLIO BELETTI, JULIANA MARTINS DA SILVA GALLO, PATRÍCIA TERRA ALVES, SABRINA VAZ DOS SANTOS E SILVA, CARLOS UEIRA-VIEIRA D - 122 BOLA (BOVINE ANTIGEN LEUKOCYTE) TRANSCRIPTS IN BOVINE BLASTOCYSTS AND COTYLEDONS MARCELO EMÍLIO BELETTI, JULIANA MARTINS DA SILVA GALLO, CARLOS UEIRA-VIEIRA, PATRÍCIA TERRA ALVES, SABRINA VAZ DOS SANTOS E SILVA D - 123 ALTERATIONS IN WISTAR RATS FERTILITY BY RESTRICTION OF PARADOXICAL SLEEP. ROMUALDO MORANDI FILHO, LARA IZABELLA FRANCO MARIANO, ADRIANO LARA ZUZA, MOLINE SEVERINO LEMOS, MARCELO EMILIO BELETTI D - 124 STUDY OF THE NUCLEOLAR CYCLE OF TRIATOMA JUAZEIRENSIS (HEMIPTERA: TRIATOMINAE) KAIO CESAR CHABOLI ALEVI, PRISCILA PASQÜETTO MENDONÇA, NATHÁLIA PAIVA PEREIRA, NAYARA FERNANDA DA COSTA CASTRO, LARISSA CENTURION GANDOLPHI, MARIA TERCÍLIA VILELA DE AZEREDO OLIVEIRA D - 108 ANALYSIS WITH RESTRICTION ENDONUCLEASE HAEIII IN TRIATOMINAE (HETEROPTERA, REDUVIIDAE) LARISSA CENTURION GANDOLPHI, ADAUTO DE OLIVEIRA BORGUETI, ROSANA SILISTINO-SOUZA, NAYARA FERNANDA DA COSTA CASTRO, MARIA TERCÍLIA VILELA DE AZEREDO OLIVEIRA D - 125 GENE TWO OF NON-CLASSICAL BOVINE ANTIGEN LEUKOCYTE (BOLA) TRANSCRIPTS QUANTIFICATION IN FETAL BOVINE PLACENTA LAYS OLIVEIRA ROCHA, JULIANA MARTINS DA SILVA GALLO, CARLOS UEIRA-VIEIRA, PATRÍCIA TERRA ALVES, SABRINA VAZ DOS SANTOS E SILVA, MARCELO EMÍLIO BELETTI D - 109 GERM CELLS DIFFERENTIATION IN VITRO: EXPLORING THE ROLE OF EXTRACELLULAR MATRIX. MARISTELA TALIARI PIMENTA, CATARINA SEGRETI PORTO, MARIA DE FÁTIMA MAGALHÃES LAZARI D - 126 EFFECTS OF STRESS BY PARADOXICAL SLEEP RESTRICTION ON WISTAR RATS’ TESTIS. LARA IZABELLA FRANCO MARIANO, ROMUALDO MORANDI FILHO, ADRIANO LARA ZUZA, MARCELO EMILIO BELETTI D - 110 PRESENCE OF URETHRAL CORPUS SPONGIOSUM AND PROSTATE (SKENE’S PARAURETHRAL GLANDS) IN FEMALE COATI THELMA MICHELLA SADDI, FLÁVIO DE REZENDE GUIMARÃES, MANOEL FRANCISCO BIANCARDI, EUGÊNIO GONÇALVES DE ARAÚJO, SEBASTIÃO ROBERTO TABOGA, WILIA MARTA ELSNER DIEDERICHSEN DE BRITO, FERNANDA CRISTINA ALCANTARA DOS SANTOS D - 127 RELATIONSHIP BETWEEN TUMOR NECROSIS FACTOR ALPHA AND BOVINE BLASTOCYSTS PRODUCED IN VITRO ROMUALDO MORANDI FILHO, JULIANA MARTINS DA SILVA GALLO, CARLOS UEIRA-VIERA, PATRÍCIA TERRA ALVES, MARCELO EMÍLIO BELETTI D - 128 COMPONENT OF BIRTH CONTROL PILL ADMINISTERED DURING GESTATION AND PUBERTY ALTERS THE MORPHOPHYSIOLOGY OF MALE AND 104 FEMALE PROSTATE OF SENIL GERBIL ANA PAULA DA SILVA PEREZ, MANOEL FRANCISCO BIANCARDI, LUIZ ROBERTO FALLEIROS JUNIOR, FERNANDA CRISTINA ALCÂNTARA DOS SANTOS, SEBASTIÃO ROBERTO TABOGA D - 129 DIABETES AND GLYCEMIC CONTROL: STEM CELL REACTIVITY IN THE PROSTATIC MICROENVIRONMENT RAÍSA MISTIERI LORENCINI, AMANDA CIA HETZL, EDUARDO MARCELO CÂNDIDO, FABIO MONTICO, LARISSA AKEMI KIDO, WAGNER JOSÉ FÁVARO, VALÉRIA HELENA ALVES CAGNON D - 130 MORPHOLOGICAL CHARACTERIZATION OF TOROIDAL STRUCTURES IN SPERM CHROMATIN OF BULL, TURKEY, AND HUMAN ADRIANO LARA ZUZA, ELISSON TERÊNCIO SOUZA, ROMUALDO MORANDI FILHO, MARCELO EMÍLIO BELETTI D - 131 ROLE OF THE ENTHESIS ON PUBIC SYMPHYSIS RECOVERY DURING POSTPARTUM BIANCA GAZIERI CASTELUCCI, SÍLVIO ROBERTO CONSONNI, VIVIANE DE SOUZA ROSA, HENRIQUE MARQUES-SOUZA, PAULO PINTO JOAZERIO D - 132 EFFECT OF LOW PROTEIN DIET (CASEIN 8%) DURING THE INTRAUTERINE AND LACTATIONAL PERIODS ON TUBULAR LUMENATION AND GERM CELLS DEVELOPMENT IN THE TESTIS OF IMMATURE WISTAR RATS JESSICA SANTANA DE OLIVEIRA, ALLUANAN ADELSON DO NASCIMENTO SILVA, OLÁVIO CAMPOS JUNIOR, FERNANDA CAROLINA RIBEIRO DIAS, CAROLINA PEIXOTO MAGALHÃES, SANDRA LOPES SOUSA, ELIZABETH NEVES DE MELO D - 133 EXPOSURE TO TESTOSTERONE DURING PRENATAL LIFE INCREASES THE SUSCEPTIBILITY TO THE DEVELOPMENT OF PROSTATIC LESIONS IN OLD FEMALE GERBIL (MERIONES UNGUICULATUS) MANOEL FRANCISCO BIANCARDI, ANA PAULA SILVA PEREZ, LUIZ ROBERTO FALLEIROS-JR, FERNANDA CRISTINA ALCANTARA DOS SANTOS, REJANE MAIRA GÓES, SEBASTIÃO ROBERTO TABOGA D - 134 ACUTE EFFECT OF THE FUNGICIDE MANCOZEB ON DIAMETER OF THE NUCLEUS AND NUMBER OF ESPERMATOGENIC CELLS AND SERTOLI CELLS FROM THE FRUGIVOROUS BATS ARTIBEUS LITURATUS (OLFERS, 1818) DIANE DA CRUZ MIRANDA, TÚLIO FIORINI CARVALHO, RAQUEL ARMINDA CARVALHO MACHADO, DANIELA VALENTE DE ANDRADE, MIRLAINE SOARES BARROS, ALESSANDRO BRINATI, MARIANA MACHADO NEVES, MARIELLA BONTEMPO DUCA DE FREITAS, SÉRGIO LUIS PINTO DA MATTA D - 135 EFFECT OF THE FUNGICIDE TEBUCONAZOLE ON DIAMETER OF THE NUCLEUS AND NUMBER OF ESPERMATOGENIC CELLS AND SERTOLI CELLS FROM THE FRUGIVOROUS BATS ARTIBEUS LITURATUS (OLFERS, 1818) DIANE DA CRUZ MIRANDA, TÚLIO FIORINI CARVALHO, RAQUEL ARMINDA CARVALHO MACHADO, DANIELA VALENTE DE ANDRADE, MIRLAINE SOARES BARROS, ALESSANDRO BRINATI, MARIANA MACHADO NEVES, MARIELA BONTEMPO DUCA DE FREITAS, SÉRGIO LUÍS PINTO DA MATTA D - 136 USING FLUORESCENT MARKERS FOR ASSESSING SPERM DAMAGE IN COLLARED PECCARIES (TAYASSU TAJACU) CAUSED BY SEMEN CRYOPRESERVATION MARIANA DE ARAÚJO DA SILVA, GISLAYNE CHRISTIANNE XAVIER PEIXOTO, THIBÉRIO DE SOUZA CASTELO, MOACIR FRANCO DE OLIVEIRA, GABRIELA LIBERALINO LIMA, JOSÉ ARTHUR BRILHANTE BEZERRA, ALEXANDRE RODRIGUES SILVA D - 137 PROLONGED BISPHENOL-A EXPOSURE STIMULATES EPITHELIAL PROLIFERATION WHICH LEADS TO THE DEVELOPMENT OF A HYPERPLASIC GLAND IN THE ADULT FEMALE GERBIL MÔNICA SOUSA CAMPOS, MANOEL FRANCISCO BIANCARDI, ANDRÉ VILELA GALVÃO, RODRIGO FERNANDES DE LIMA, JOSIANE FAGANELLO, MARA RÚBIA MARQUES, FERNANDA CRISTINA ALCÂNTARA DOS SANTOS, LUIZ ROBERTO FALLEIROS-JR, SEBASTIÃO ROBERTO TABOGA D - 138 REPRODUCTIVE TOXICITY OF MULTI-WALLED CARBON NANOTUBES IN MICE. ALEXANDRA NAVA, SOLANGE CRISTINA DA SILVA MARTINS HOELZEL, SILVANE SOUZA ROMAN D - 139 EXPRESSION OF CHEMOKINE (C-C MOTIF) LIGAND 25 DURING MOUSE EMBRYO IMPLANTATION RODRIGO BARBANO WEINGRILL, M. S. HOSHIDA, C. D. MARTINHAGO, E. BEVILACQUA D - 140 DEVELOPMENT OF A CHIMERIC EQUID TESTIS USING THE CELL AGGREGATE XENOGRAFTING APPROACH G.F. AVELAR, G.M.J. COSTA, J.V. REZENDE-NETO, S.M.S.N. LACERDA, P.H.A. CAMPOS-JÚNIOR, B.S.C. ANDRADE, L.R. FRANÇA E – Cell Biology and Education E1-E12 E -1 CONSTRUCTION OF DIDACTIC-PEDAGOGICAL MODELS FOR CELL BIOLOGY TEACHING JOÃO PAULO FERREIRA SCHOFFEN, ISABELLA MARIA DIAS PAYÃO ORTIZ, JEAN LUCAS KREMER, PRISCILA EUNICE DA SILVA, MATHEUS EDUARDO LEME, ANA CAROLINA CORREIA AMBRÓSIO, FRANCIELY PALIARIN, CARLOS VINÍCIUS DALTO DA ROSA, KARLA FERNANDA FERRAZ, LUCILENE CRISTINA DA SILVA, ALINE NAZARENO E -2 CONSTRUCTION OF DIDACTIC-PEDAGOGICAL MODELS FOR EMBRYOLOGY TEACHING JOÃO PAULO FERREIRA SCHOFFEN, CRISTINA ALVES FONSECA, ISABELLA MARIA DIAS PAYÃO ORTIZ, YURI FUJIMORI, BEATRIZ MAYARA LIMA OKISHI, TAYEME CRISTINA PIVA, ROSANA CAMPOS PASCHOALINO, JULIANA HADDAD, FABIANA APARECIDA MARTINS, LUIZ EDUARDO LOURENÇO SOUZA E -3 THREE-DIMENSIONAL CELL MODELS AS A TEACHING TOOL FOR CELL BIOLOGY COURSE IN THE BACHELOR IN SCIENCE AND TECHNOLOGY (BC&T) AT UNIVERSIDADE FEDERAL DO ABC (UFABC) ARNALDO R. SANTOS JR., RENATA SIMÕES, MARCELLA P. MILAZZOTTO, CHRISTIANE B. LOMBELLO E -4 CELL BIOLOGY IN THE PROCESSES OF SCIENTIFIC RESEARCH IN PUBLIC HIGH SCHOOL TATIANE DA AQUINO, PAULO RICARDO DA ROSA, JULIANE MEILI, TANIA BERNHARD E -5 DESCRIPTION OF A PEDAGOGICAL EXPERIMENT ON BUILDING UNDERGRADUATE STUDENTS’ CLINICAL THINKING COUPLED UP WITH CELLULAR PROCESSES AND BIOCHEMISTRY UNDERSTANDING LETICIA VARGAS DE MESQUITA, CAROLINA ÁVILA DE ALMEIDA, CARLOS EDUARDO ABBUD HANNA ROQUE, RICARDO FELIPE ALVES MOREIRA, CRISTIANE BARBOSA ROCHA E -6 IN VIVO OR IN CITO: CAN THE CELL REPLACE THE ANIMAL? – A DOCUMENTARY BEATRIZ LOUREIRO DE SOUZA, RÓBER BACHINSKI, LETÍCIA APARECIDA BARBOSA HUMMEL, GUTEMBERG GOMES ALVES E -7 PRACTICAL LESSONS IN THE LABORATORY AS A PROPOSAL TO BETTER TEACH CELL BIOLOGY TO HIGH SCHOOL STUDENTS FERNANDA SILVA DE SOUZA, LACI GONÇALVES VIANA, RENATO AUGUSTO DAMATTA E -8 OPINION ON THE USE AND IMPORTANCE OF PRACTICAL LESSONS AND THE EFFECT OF A CONTINUOUS EDUCATION COURSE IN CELL BIOLOGY CONCEPTS TO HIGH SCHOOL TEACHERS FROM THE NORTHERN STATE OF RIO DE JANEIRO. ALINE BRANDÃO ALVES LIMA, FERNANDA SILVA DE SOUZA, RENATO AUGUSTO DAMATTA E -9 AN INTEGRATED PRACTICAL COURSE ON CELL BIOLOGY BASED ON A BIOMATERIAL CYTOCOMPATIBILITY PROJECT FOR HEALTH/BIOLOGY UNDERGRADUATE STUDENTS DANIELA COSTA SILVA, JULIANA ALVES CÔRTES, ROBER FREITAS BACHINSKI, GUILHERME LECHUGAR, CAROLINA NASCIMENTO SPIEGEL, GUTEMBERG GOMES ALVES E -10 GETTING OUT OF THE CAMPUS! PRESENTING THE PROGRESS OF RESEARCH IN CELL BIOLOGY FOR HIGH SCHOOL TEACHERS BÁRBARA CAPITANIO DE SOUZA, ANNA CHRISTINA MEDEIROS FOSSATI, LENIR ORLANDI PEREIRA, SIMONE MARCUZZO, RUI FERNANDO FELIX LOPES, TATIANA LUFT, EMERSON CASALI, MARCELO LAZZARON LAMERS E -11 EDUCATION WITH TECHNOLOGY: THE USE OF AN ADAPTIVE INTERFACE FACILITATES THE TEACHING AND LEARNING OF CELL BIOLOGY. MAYARA LUSTOSA DE OLIVEIRA, HERNANDES FAUSTINO DE CARVALHO E -12 “CELLULAR DOMINATION”: A POWERFUL TOOL FOR TEACHING CELL BIOLOGY KRISIA EMANUELLE FERREIRA DA SILVA, THAÍS PRISCILA DE SOUZA TORRES, MARLLON ALEX NASCIMENTO SANTANA, JÉSSICA ANDRÉIA PEREIRA BARBOSA, JEYMESSON RAPHAEL CARDOSO VIEIRA F – Cell Cycle and Proliferation F1-F27 F-1 PMA ANTAGONISTIC EFFECTS ON PROLIFERATION OF IMMORTALIZED HUMAN HACAT KERATINOCYTES: STIMULATION OF NORMAL AND INHIBITION OF H-RASV12-TRANSFORMED CELLS JULIANNA DIAS ZEIDLER, HUGO AGUIRRE ARMELIN F-2 P53, KI-67, COX-2 EXPRESSION IN RAT TONGUE EXPOSED TO STEROIDS RENAN POZZI, KELLY ROSSETTI FERNANDES, CAROLINA FOOT GOMES MOURA, ANA CLAUDIA MUNIZ RENNO, DANIEL ARAKI RIBEIRO F-3 EFFECT OF UVA AND UVB ON THE NEURONS’ CELL CYCLE OF THE CENTRAL OLFACTORY SYSTEM OF THE CRAB UCIDES CORDATUS. GABRIELA HOLLMANN, GABRIELLE DE JESUS FERREIRA, ÁLVARO LETÃO, RAFAEL LINDEN, SILVANA ALLODI F-4 THE INSULIN RECEPTOR TRANSLOCATES TO THE NUCLEUS TO REGULATE CELL PROLIFERATION AND LIVER REGENERATION. CLAUDILENE R.CHAVES, MARIA JIMENA AMAYA, MARISA C.F. CASTELUBER, DOUGLAS L. ALMEIDA, MAURO C.X. PINTO, LILIAN A.M. ARANTES, LIDIA MARIA ANDRADE, ANA C.N. PINHEIRO, EMERSON A. FONSECA, GUSTAVO B. MENEZES, ANA MARIA DE PAULA, RODRIGO RIBEIRO RESENDE, MICHAEL H. NATHANSON, MARIA DE FÁTIMA LEITE F-5 EFFECTS OF LOW INTENSITY RED LASER ON BACTERIAL GROWTH AND PLASMIDS DNA JULIANA NOGUEIRA DOS SANTOS, CAMILA ROOS, FLAVIA DE 105 PAOLI, OSCAR ROBERTO GUIMARÃES, MAURO GELLER, ADENILSON DE SOUZA DA FONSECA F-6 GENOTOXIC EFFECTS ON ERYTHROCYTES OF TILAPIA (OREOCHROMIS NILOTICUS) EXPOSED CADMIUM AND AFTER DEPURATION JULIANA MOREIRA MENDONÇA GOMES, HEDER JOSÉ RIBEIRO, MARCELA SANTOS PROCÓPIO, JOSÉ DIAS CORRÊA JUNIOR F-7 POSSIBLE ACTIVATION OF THE WNT/BETA-CATENIN SIGNALING PATHWAY IN HYPERPLASIC PANCREATIC ISLETS OF PRE-DIABETIC MICE DANIELA APARECIDA MASCHIO, RICARDO BELTRAME OLIVEIRA, CAROLINA PRADO DE FRANÇA CARVALHO, CARLA BEATRIZ COLLARES-BUZATO, MARIANE RODRIGUES DOS SANTOS F-8 DECREASED CX36 ISLET CONTENT AND IMPAIRED COMPENSATORY BETA CELL FUNCTION AND GROWTH IN RESPONSE TO HIGH FAT DIET IN LDL RECEPTOR KNOCKOUT MICE RICARDO BELTRAME OLIVEIRA, CAROLINA PRADO DE FRANÇA CARVALHO, DANIELA APARECIDA MASCHIO, ANTÔNIO CARLOS BOSCHERO, HELENA COUTINHO FRANCO DE OLIVEIRA, CARLA BEATRIZ COLLARESBUZATO F-9 DIFFERENTIAL LIPID ACCUMULATION IN BONE MARROW STROMAL CELLS AND ITS INFLUENCE ON HEMATOPOIESIS IN VITRO. GABRIEL FERRAZ DA SILVA, ANDERSON JUNGER TEODORO, GEORGIA CORREA ATELLA, MARCELO EINICKER LAMAS, ALEX BALDUINO DE SOUZA, RADOVAN BOROJEVIC, MARCIA CURY EL-CHEIKH F - 10 IL-4 MODULATES THE PROLIFERATIVE EFFECT OF EGF IN RETINAL CELLS GUSTAVO MATARUNA DA SILVA, LUIS EDUARDO GOMES BRAGA, ELIZABETH GIESTAL DE ARAUJO F - 11 EVALUATION OF IRON SUPPLEMENTED MEDIA CULTURE IN THE CELLULAR VIABILITY AND GENOMIC STABILITY OF CELL LINES – MRC5 AND HEPG2 ANA LÚCIA VARGAS ARIGONY CORTE, LARISSA MILANO, MICHELLE LIMA, ANDRE JUNCHEM, CRISTIANO TRINDADE, MIRIANA MACHADO, DIANA BORDIN, EDUARDO FILIPPI-CHIELA, GUIDO LENZ, DANIEL PRA, JOÃO ANTONIO PÊGAS HENRIQUES F - 12 SIGNS OF CELLULAR SENESCENCE IN ESTROGEN-INDUCED PITUITARY HYPERPLASIA MARÍA EUGENIA SABATINO, JUAN PABLO PETITI, LILIANA DEL VALLE SOSA, SILVINA GUTIÉRREZ, ALEXANDRA SUSANA LATINI, ALICIA INÉS TORRES, ANA LUCÍA DE PAUL F - 23 SIGNALING TRIGGERED BY THE NEUROPEPTIDE PACAP AND SHH INTERACT IN THE CONTROL OF CELL PROLIFERATION IN THE DEVELOPING RETINA THALINE DAIANNE FARIAS ALVES DE LIMA, BRIAN NJAINE, RAFAEL LINDEN, MARIANA SOUZA DA SILVEIRA F - 24 DOES 5-BROMO-2'-DEOXYURIDINE (BRDU) INFLUENCE THE POSTNATAL DEVELOPMENT IN RATS? LÍVIA CLEMENTE MOTTA TEIXEIRA, SILVIA HONDA TAKADA, VITOR YONAMINE LEE, MARIA INÊS NOGUEIRA, GILBERTO FERNANDO XAVIER F - 25 ENDOSYMBIOSIS IN TRYPANOSOMATIDS: THE SYMBIOTIC BACTERIUM UNDERGOES COORDINATED DIVISION WITH THE HOST CELL STRUCTURES. CAROLINA MOURA COSTA CATTA PRETA, FELIPE LOPES BRUM DA SILVEIRA, CAMILA CRISTINA DA SILVA, SERGIO SCHENKMAN, MARIA CAROLINA ELIAS, LUCIANA CIAPINA, LUIZ GONZAGA, ANA TEREZA VASCONSELOS, WANDERLEY DE SOUZA, MARIA CRISTINA MACHADO MOTTA F - 26 BALANCE OF ADIPOGENESIS AND APOPTOSIS MARKERS IN MESENTERIC ADIPOSE TISSUE DURING THE DEVELOPMENT OF CANCER CACHEXIA FELIPE DE OLIVEIRA FRANCO, FELIPE DOS SANTOS HENRIQUES, KALTINAITIS BENETON NUNES HYPOLITO DOS SANTOS, PÂMELA VIEGAS KNÖBL, RODRIGO XAVIER DAS NEVES, ROGERIO ANTONIO LAURATO SERTIÉ, CLAUDIO SABURO SHIDA, SIDNEY BARNABÉ PERES, MIGUEL LUIZ BATISTA JÚNIOR F - 27 THE EFFECTS OF 7-EPI-CLUSIANONE ON PROLIFERATIVE BEHAVIOR OF HEPG2 CELLS IZABELLA LUIZ SUZUKI, EVANDRO LUÍS DE OLIVEIRA NIERO, GLAUCIA MARIA MACHADO SANTELLI, MARCELO HENRIQUE DOS SANTOS, MARISA IONTA G – Cell Death G1-G38 G-1 MITOCHONDRIAL ENERGY UTILIZATION IN YOUNG AND OLD WORKER HONEYBEES (APIS MELLIFERA) HSU CHIN-YUAN, YU-LUNG CHUANG G-2 CELLULAR DEGRADATION ACTIVITY IN YOUNG AND OLD WORKER HONEYBEES (APIS MELLIFERA) CHAN YU-PEI, CHUANG YU-LUNG, HSU CHIN-YUAN F - 13 THE EFFECTS OF CAMPTOTHECIN AND BERENIL ON ULTRASTRUCTURE OF TRYPANOSOMA CRUZI EPIMASTIGOTE FORM ALINE ARAUJO ZUMA, MARIA CAROLINA ELIAS, WANDERLEY DE SOUZA, MARIA CRISTINA MACHADO MOTTA G-3 EFFECTS OF DISTURBED ENVIRONMENTS IN THE LIVER OF PROCHILODUS LINEATUS BRUNO FIORELINI PEREIRA, JOSÉ ALGUSTO SENHORINI, RITA DE CÁSSIA GIMENES DE ALCÂNTARA ROCHA, FLAVIO HENRIQUE CAETANO F - 14 TRANSFORMING GROWTH FACTOR BETA1 PROMOTES P27KIP1 POST-TRANSCRIPTIONAL REGULATION IN GASTRIC CELLS OF SUCKLING RATS ANA PAULA ZEN PETISCO FIORE, EUNICE RIBEIRO DE ANDRADE SÁ, LUCIANA HARUMI OSAKI, CRUZ ALBERTO MENDOZA RIGONATTI, PATRICIA GAMA G-4 CHARACTERIZATION OF DUAL EFFECTS INDUCED BY ANTIMICROBIAL PEPTIDES: REGULATED CELL DEATH OR MEMBRANE DISRUPTION EDGAR JULIAN PAREDES GAMERO, MARTA NC MARTINS, FÁBIO AM CAPPABIANCO, JAIME S IDE, ANTIONIO DE MIRANDA F - 15 EVALUATION OF GASTRIC CANCER CELL PROLIFERATION AND ADHESION IN CELL CULTURE PLATES SUBMITTED TO CORONA AND GLOW DISCHARGE (PLASMA) PROCESSING WITH DIFFERENT N2/H2 RATIO RAQUEL AYRES, FERNANDO BONATTO, DIEGO BONATTO G-5 THE PARACRINE SIGNALING BY ATP/ ADP IS DETRIMENTAL DURING THE STERILE CELL DEATH INDUCED BY ACETAMINOPHEN SYLVIA STELLA AMARAL, PEDRO ELIAS MARQUES PEREIRA SILVA, LAURA LOPES NOGUEIRA PINTO, DANIELE ARAÚJO PIRES, LÍDIA MARIA DE ANDRADE, MARIA DE FÁTIMA LEITE, GUSTAVO BATISTA DE MENEZES F - 16 CONSTITUTIVELY ACTIVE CDK1 TRIGGERS DNA DAMAGE AND CELL CYCLE ARREST PATRÍCIA RENCK NUNES, KASIM DIRIL, PHILIPP KALDIS F - 17 GHRELIN AND GROWTH HORMONE SECRETAGOGUE RECEPTOR DISTRIBUTION IN THE GASTRIC MUCOSA OF EARLY WEANED RATS: EFFECTS ON EPITHELIAL CELL PROLIFERATION. DANIELA OGIAS, NATALIA BITTAR RODRIGUES, LUCIANA HARUMI OSAKI, CRUZ ALBERTO MENDOZA RIGONATTI, PATRICIA GAMA F - 18 THE LEISHMANIA (L.) AMAZONENSIS CELL CYCLE: WHO DUPLICATES FIRT, KINETOPLAST OR NUCLEUS? MARCELO SANTOS DA SILVA, JOMAR PATRÍCIO MONTEIRO, ARINA MARINA PEREZ, MARIA CAROLINA ELIAS, MARIA ISABEL NOGUEIRA CANO F - 19 ACTIVATION OF C-JUN N-TERMINAL KINASE (JNK) DURING MITOSIS IN RETINAL PROGENITOR CELLS. VINICIUS DE TOLEDO RIBAS, BRUNO DE SOUZA GONÇALVES, RAFAEL LINDEN, LUCIANA BARRETO CHIARINI F - 20 7-EPI-CLUSIANONE EXERTS A NEGATIVE EFFECT ON CELL CYCLE PROGRESSION IN LUNG CARCINOMA CELLS TATIANE HELENA BATISTA, NATHALIE NUNES DIAS, EVANDRO LUIS DE OLIVEIRA NIERO, MARISI GOMES SOARES, GLAUCIA MARIA MACHADO-SANTELLI, MARCELO HENRIQUE DOS SANTOS, MARISA IONTA F - 21 LEISHMANIA AMAZONENSIS RBP38 IS PROBABLY INVOLVED IN DNA DAMAGE RESPONSE IN THE NUCLEUS AND IN THE KINETOPLAST ARINA MARINA PEREZ, MARCELO SANTOS DA SILVA, PAULO VINICIUS DA MATA MADEIRA, BÁRBARA MORAES SOUZA, JULIA P. C. DA CUNHA, MARIA ISABEL NOGUEIRA CANO F - 22 CYTOTOXIC ACTIVITY OF 7-EPI-CLUSIANONE, A TETRAPRENYLATED BENZOPHENONE, ON BREAST CANCER CELL LINES. NATALIA GABRIELE HOSCH, IARA AUANA SALES, EVANDRO LUÍS DE OLIVEIRA NIERO, GLAUCIA MARIA MACHADO-SANTELLI, MARCELO HENRIQUE DOS SANTOS, MARISA IONTA G-6 TRICHOMONAS VAGINALIS: ULTRASTRUCTURAL ALTERATIONS INDUCED BY BPQ-OH COMPARED TO METRONIDAZOLE AND EVALUATION OF ITS CYTOTOXICITY IN MAMMALIAN CELLS DEBORA ROCHA AFONSO SILVA, IVONE ROSA DE ANDRADE, WANDERLEY DE SOUZA, JULIO URBINA, MARLENE BENCHIMOL G-7 ANTI-APOPTOTIC HSP27 AND ITS TRANSCRIPTION FACTOR HSTF1 ARE UP-REGULATED BY PROLACTIN IN HUMAN PANCREATIC ISLETS ROSANGELA APARECIDA WAILEMANN MANSANO, LETÍCIA FERREIRA TERRA, MARI CLEIDE SOGAYAR, LETÍCIA LABRIOLA G-8 CRUDE EXTRACT OF ANNONA MUCOSA (ANNONACEAE) CAUSES CHANGES IN CELLS OF THE GUT OF AEDES AEGYPTI LARVAE (DIPTERA: CULICIDAE) JAMILE FERNANDA SILVA COSSOLIN, MARILZA DA SILVA COSTA, JOSÉ EDUARDO SERRÃO, MÔNICA JOSENE BARBOSA PEREIRA G-9 MORPHOLOGICAL ASPECTS OF THE GUT REPLACEMENT IN BRADYSIA HYGIDA (DIPTERA: SCIARIDAE) DURING METAMORPHOSIS THAYLISE DE CASSIA SANTOS PRZEPIURA, JOSÉ ROSA GOMES, CRISTINA LÚCIA SANT’ANNA COSTA-AYUB, MARIA ALBERTINA DE MIRANDA SOARES G - 10 ANALYSIS OF PROTEIN EXPRESSION AFTER PHOTODYNAMIC THERAPY IN THE PROTOZOAN PARASITE TRITRICHOMONAS FOETUS SUSANE MOREIRA MACHADO, CRISTINA PACHECO SOARES, CRISTIANE APARECIDA FERREIRA PIRES, FERNANDA ROBERTA MARCIANO, ANDERSON OLIVEIRA LOBO, NEWTON SOARES DA SILVA G - 11 GOLD NANOPARTICLES DO NOT INDUCE CYTOTOXICITY IN THE ALVEOLAR TYPE-II CELL LINE CAROLINA DA SILVA GOUVEIA PEDROSA, TALÍRIA SILVA LOPES, KARINA RIBEIRO DA SILVA, LEANDRA SANTOS BAPTISTA, PRISCILA FALAGAN LOTSCH, GUSTAVO CONDE MENEZES, RADOVAN BOROJEVIC, JOSÉ MAURO GRANJEIRO 106 G - 12 APOPTOSIS INDUCTION IN TUMOR CELLS BY A NATURAL INTRACELLULAR PEPTIDE CHRISTIANE BEZERRA DE ARAUJO, EDNA T. KIMURA, EMER SUAVINHO FERRO G - 30 CYTOTOXICITY OF PHENOLIC COMPOUNDS ON HUMAN SKIN CELL LINES. ANDRÉA COSTA FRUET, SILVYA STUCHI MARIA-ENGLER, SILVIA BERLANGA DE MORAES BARROS G - 13 EFFECTS OF THE BOTHROPOIDES INSULARIS VENOM (AMARAL, 1921) ON RENAL TUBULAR CELLS. CLARISSA PERDIGÃO MELLO, LOUISE DONADELLO TESSAROLO, ALBA FABÍOLA COSTA TORRES, RAMON RÓSEO PAULA PESSOA BEZERRA DE MENEZES, GUSTAVO JOSÉ DA SILVA PEREIRA, ISABEL CRISTINA OLIVEIRA DE MORAIS, DÂNYA BANDEIRA LIMA, JÁDER ALMEIDA CANUTO, ALICE MARIA COSTA MARTINS, SORAYA SOUBHI SMAILI G - 31 APOPTOTIC RATES IN WISTAR RAT KIDNEYS TREATED WITH CYCLOSPORIN A AND HETEROPTERYS TOMENTOSA. KARINE MOURA FREITAS, MARIA APARECIDA DA SILVA DIAMANTE, JACQUELINE MERIELLEN DE ALMEIDA, NAYARA RUDECK OLIVEIRA STHEL COCK, FABRICIA DE SOUZA PREDES, MARÇAL AMICE JORGE, HEIDI DOLDER G - 14 THE EFFECT OF EARLY WEANING ON APOPTOSIS IN THE GASTRIC EPITHELIUM OF RATS IN POSTNATAL DEVELOPMENT CAMILA KAMLA MARTINATTI, PATRÍCIA GAMA, CRUZ ALBERTO MENDONZA RIGONATI, LUCIANA HARUMI OSAKI G - 15 ANALYSIS OF GOLD NANOPARTICLES TOXICITY IN HUMAN FETAL LUNG FIBROBLAST (MRC5) CELL CULTURE TALÍRIA SILVA LOPES, PRISCILA FALAGAN LOTSCH, CAROLINA DA SILVA GOUVEIA PEDROSA, KARINA RIBEIRO DA SILVA, LEANDRA SANTOS BAPTISTA, GUSTAVO CONDE MENEZES, JOSE MAURO GRANJEIRO G - 16 PHENOTHIAZINE-INDUCED HEPG2 CELL DEATH: STRUCTUREACTIVITY RELATIONSHIP AND MITOCHONDRIAL INVOLVEMENT PRISCILA AFONSO DE FARIA, FELIPE SAMUEL PESSOTO, EDGAR JEAN PAREDES-GAMERO, TIAGO RODRIGUES G - 17 EFFECT OF PROTEIN MALNUTRITION IN THE MECHANISMS OF APOPTOSIS, NECROSIS AND AUTOPHAGY IN MARROW HYPOPLASIA. JACKELINE SOARES DE OLIVEIRA BELTRAN, GRAZIELA BATISTA DA SILVA, DALILA CUNHA DE OLIVEIRA, ED WILSON DOS SANTOS, PRIMAVERA BORELLI G - 18 DENGUE INFECTION RESULTS IN CELL DEATH OF HUMAN BRAIN MICROVASCULAR ENDOTHELIAL CELLS MICHELLE PREMAZZI PAPA, LUCIANA BARROS DE ARRUDA G - 19 THE PROTECTIVE EFFECT AGAINST PHOTODAMAGE OF ALOE BARBADENSIS MIL IN HUMAN KERATINOCYTES ANA CLAUDIA VIOTTO, NAYRA FERNANDES SANTOS, CLEIDIANE SOUZA, DIVINOMAR SEVERINO, MAURÍCIO SILVA BAPTISTA, WALESKA KERLLEN MARTINS G - 20 INVOLVEMENT OF AUTOPHAGY IN THE PHENOTHIAZINE-INDUCED APOPTOSIS IN K562 CELLS VIVIAN MATSUKURA DOS SANTOS, FABIO D. NASCIMENTO, GISELLE ZENKER JUSTO, IVARNE LUIS DOS SANTOS TERSARIOL, TIAGO RODRIGUES G - 21 IN THE SEARCH FOR SPECIFIC MECHANISMS OF PHOTO-INDUCED CELL DEATH NAYRA FERNANDES SANTOS, ISABEL DE OLIVEIRA LIMA BACELLAR, ANA CLÁUDIA VIOTTO, CHRISTIANE PAVANI, WALESKA KERLLEN MARTINS, MAURÍCIO DA SILVA BAPTISTA G - 22 EFFECTS OF BOTHROPS MARAJOENSIS SNAKE VENOM AND ITS FRACTION PHOSPHOLIPASE A2 ON RENAL TUBULAR CELLS AND MURINE MACROPHAGES GDAYLLON CAVALCANTE MENESES, JÁDER ALMEIDA CANUTO, LOUISE DONADELLO TESSAROLO, LÍVIA CORREIA FERNANDES, ALBA FABÍOLA COSTA TORRES, TICIANA PRACIANO PEREIRA, JOSIANE S. QUETZ, HELENA SERRA AZUL MONTEIRO, ALICE MARIA COSTA MARTINS G - 23 PALLADACYCLE-INDUCED CASPASE-DEPENDENT APOPTOSIS IN K562 LEUKEMIA CELLS IS ASSOCIATED TO THIOL OXIDATION AND MITOCHONDRIAL DEPOLARIZATION VIVIAN WERLOGER RODRIGUES DE MORAES, EDGAR JULIAN PAREDES-GAMERO, TIAGO RODRIGUES G - 24 POLYMERIC MICELLAR SYSTEM POTENTIATES THE ANTITUMOR ACTIVITY OF PHENOTHIAZINES IN HUMAN K562 LEUKEMIA CELLS JOYCE CRISTINE DE MELLO, DEYSE CARDOSO DE SILVA, DANIELE RIBEIRO DE ARAÚJO, TIAGO RODRIGUES G - 25 NEEM SEED OIL EXHIBITS CONCENTRATION DEPENDENT DUAL EFFECTS ON HEPG2 CELL VIABILITY: PROTECTION AGAINST OXIDATIVE STRESS AND INDUCTION OF CELL DEATH PALOMA CAROLINE RIBEIRO, TIAGO RODRIGUES G - 26 RENAL EFFECTS OF DINOPONERA QUADRICEPS VENOM ALBA FABIOLA COSTA TORRES, JÁDER ALMEIDA CANUTO, LOUISE DONADELO TESSAROLO, TICIANA PRACIANO PEREIRA, ANTONIO RAFAEL COELHO JORGE, MILEYDE PONTE PORTELA, YVES PATRIC QUINET, HELENA SERRA AZUL MONTEIRO, ALICE MARIA MARTINS G - 27 PLUMBAGIN-INDUCED CELL DEATH IN LEUKEMIA MODEL IS PROMOTED BY CELLULAR OXIDATIVE UNBALANCE MAYARA KAORI KISAKI, TIAGO RODRIGUES G - 28 SEX-RELATED DIFFERENCES ON HIPPOCAMPAL MITOCHONDRIAL PROFILE INDUCED BY NEONATAL HYPOXIA-ISCHEMIA INJURY ANA PAULA TONIAZZO, SIMONE NARDIN WEIS G - 29 SEX-SPECIFIC EFFECTS OF AUTOPHAGY CELL DEATH IN NEONATES FOLLOWING HYPOXIA-ISCHEMIA SIMONE NARDIN WEIS, ANA PAULA TONIAZZO, BRADLEY P. ANDER, XINHUA ZHAN, MILO CAREAGA, PAUL ASHWOOD, FRANK RAY SHARP, ANGELA TEREZINHA DE SOUZA WYSE, CARLOS ALEXANDRE NETTO G - 32 REDUCTION OF COLLAGEN FIBERS AND CELL DEATH BY APOPTOSIS IN THE LAMINA PROPRIA DURING STAGES OF TOOTH ERUPTION JOSÉ PAULO DE PIZZOL JÚNIOR, ESTELA SASSO CERRI, PAULO SÉRGIO CERRI G - 33 PROGRAMMED CELL DEATH IN AQUATIC BACTERIA IN TWO TROPICAL AQUATIC ECOSYSTEMS MODELS THIAGO PEREIRA DA SILVA, JULIANA GAMALIER, VICTOR ZARANTONELLO, FÁBIO ROLAND, ROSSANA CORREA NETTO DE MELO G - 34 IMPAIRED ENDOPLASMIC RETICULUM STRESS RESPONSE IN LYMPHOCYTES FROM PATIENTS WITH BIPOLAR DISORDER BIANCA PFAFFENSELLER, BIANCA WOLLENHAUPT DE AGUIAR, GABRIEL RODRIGO FRIES, GABRIELA DELEVATI COLPO, RENAN KUBIACHI BURQUE, GIOVANA BRISTOT, PÂMELA FERRARI, KEILA MENDES CERESÉR, FÁBIO KLAMT, FLÁVIO KAPCZINSKI G - 35 IN VITRO ANTI-APOPTOTIC ACTIVITY OF OIL FROM SALVIA LACHNOTASCHYS IN LYMPHOCYTES AND MACROPHAGES OF MUS MUSCULUS LUCAS WAGNER GORTZ, MARCELO BETTEGA, LUIZA FERNANDA SCHIER, OSVALDO MALAFAIA, CLAUDIA CONSUELO DO CARMO OTA G - 36 ANTI-APOPTOTIC EFFECT OF A PROTEIN ISOLATED FROM PODALIA SP (LEPIDOPTERA: MEGALOPYGIDAE) HEMOLYMPH IN VERO AND SF-9 CELLS NATHALIA DELAZERI DE CARVALHO, ROBERTO HENRIQUE PINTO MORAES, RITA MARIA ZUCATELLI MENDONÇA, RONALDO ZUCATELLI MENDONÇA G - 37 IN VITRO ANTI-APOPTOTIC ACTIVITY OF OIL FROM COPAIFERA LANGSDORFFII IN LYMPHOCYTES AND MACROPHAGES OF MUS MUSCULUS MARCELO BETTEGA, LUCAS WAGNER GORTZ, LUIZA FERNANDA SCHIER, OSVALDO MALAFAIA, CLAUDIA CONSUELO DO CARMO OTA G - 38 DC-SIGN MEDIATES DENGUE VIRUS-INDUCED PLATELET ACTIVATION, MITOCHONDRIAL DYSFUNCTION AND CELL DEATH EUGENIO DAMACENO HOTTZ, MARCUS FERNANDES OLIVEIRA, ROGÉRIO VALLS DE SOUZA, ANDRÉA THOMPSON DA POIAN, ANDREW S. WEYRICH, GUY A. ZIMMERMAN, PATRÍCIA TORRES BOZZA, FERNANDO AUGUSTO BOZZA G – 39 NFAT1 COOPERATES WITH RAS/RAF/MEK/ERK PATHWAY IN THE INDUCTION OF APOPTOTIC AND NECROTIC CELL DEATH IN FIBROBLASTS. ROBBS, B.K., VIOLA, J.P.B. H – Cell Differentiation H1-H19 H-1 THE INFLUENCE OF CORTICOSTERONE IN THE MATURATION OF RAT GASTRIC MUCOSA DURING EARLY WEANING JULIANA GUIMARÃES ZULIAN, CRUZ ALBERTO MENDOZA RIGONATI, PATRÍCIA GAMA H-2 CHOLESTEROL DEPLETION ALTERS THE EXPRESSION OF ADHESION PROTEINS AND CELL MIGRATION IN MYOBLASTS ANA CLAUDIA BATISTA POSSIDONIO, CAROLINA PONTES SOARES, DÉBORA MORUECO PORTILHO, JULIANA LOURENÇO ABRANTES, VICTOR DO VALLE PEREIRA MIDLEJ, MARLENE BENCHIMOL, CLÁUDIA DOS SANTOS MERMELSTEIN H-3 2D AND 3D-ORGANIZED CARDIAC CELLS SHOWS DIFFERENCES IN CELLULAR MORPHOLOGY, ADHESION JUNCTIONS, PRESENCE OF MYOFIBRILS AND PROTEIN EXPRESSION CAROLINA PONTES SOARES, VICTOR MIDLEJ, MARIA EDUARDA WESCHOLLEK DE OLIVEIRA, MARLENE BENCHIMOL, MANOEL LUIS COSTA, CLÁUDIA DOS SANTOS MERMELSTEIN H-4 HIGH LEVELS OF CIRCULATING TRIIODOTHYRONINE INDUCES PLASMA CELL DIFFERENTIATION IN MICE FLAVIA FONSECA BLOISE, FELIPE OLIVEIRA, ALBERTO FÉLIX NÓBREGA, ALINE CORDEIRO, LUCIANA DE SOUZA PAIVA, DENNIS D TAUB, RADOVAN BOROJEVIC, CARMEN CABANELAS PAZOS-MOURA, VALÉRIA DE MELLO-COELHO H-5 EFFECT OF THE LIPID-RAFT DISORGANIZATION ON THE MUSCULAR CELL DIFFERENTIATION IN ZEBRAFISH MODEL EDUARDO ANDRÉS RÍOS MORRIS, LAISE CAMPOS, CLAUDIA MERMELSTEIN, MANOEL LUÍS COSTA H-6 CHARACTERIZATION OF HEMATOPOIETIC STEM/PROGENITOR CELLS OBTAINED IN VITRO FROM HUMAN EMBRYONIC STEM CELLS IN COCULTURE SYSTEM WITH MOUSE EMBRYONIC FIBROBLASTS EVERTON DE BRITO OLIVEIRA COSTA, MARISTELA DELGADO ORELLANA, DANIELLE APARECIDA ROSA DE MAGALHÃES, VIRGÍNIA MARA DE DEUS WAGATSUMA, LILIAN FIGUEIREDO MOREIRA, SIMONE KASHIMA HADDAD, DIMAS TADEU COVAS, APARECIDA MARIA FONTES 107 H-7 RHOA GTPASES IS IMPORTANT FOR CELL DIFFERENTIATION IN ORAL SQUAMOUS CELL CARCINOMA NANCI MENDES PINHEIRO, MARCO AURÉLIO DE OLIVEIRA MARINHO, DOUGLAS CÔBO MICHELLI, BEATRIZ MARTINS TAVAREZ MURTA, ANA CRISTINA DE ARAÚJO LEMOS, EURÍPEDES DE OLIVEIRA MARINHO, MICHELLE TILLMMAN BIZ, VIRGÍNIA OLIVEIRA CREMA H-8 INDUCTION OF CELLULAR DIFFERENTIATION AND THE MULTIDRUG RESISTANCE PHENOTYPE MICHELE CARRETT DIAS GARCIA, LEDA KARINE DE ALMEIDA, REGINA COIMBRA ROLA, ANA PAULA DE SOUZA VOTTO, GILMA SANTOS TRINDADE H-9 ASSESSMENT OF MINERALIZATION ON PRIMARY HUMAN BONE CELLS HARVESTED FROM ARTHROPLASTY EXPLANTS WESLEY LUIZ BARROS DA SILVA, MELLODY JESSICA BALLARD ARAGÃO, EMANUELLE STELLET LOURENÇO, VINICIUS SCHOTT GAMEIRO, GUTEMBERG GOMES ALVES, JOSÉ MAURO GRANJEIRO, ADRIANA BRANDÃO RIBEIRO LINHARES H - 10 THE COMBINED USE OF RETINOIC ACID AND CAMP IS EFFECTIVE IN INDUCING DIFFERENTIATION OF HEPG2 CELLS DEBORAH ELZITA DO CARMO CORRÊA, CAMILLA CRISTINA MORI, PAULA REZENDE TEIXEIRA, GLAUCIA MARIA MACHADO SANTELLI, MARISA IONTA H - 11 B LYMPHOCYTES DIFFERENTIATE INTO PLASMA CELLS AFTER TRIIODOTHYRONINE STIMULATION IN VITRO AND EXPRESS TRBETA1 HUILA LUIZA SANTOS DA FONSECA, FLAVIA FONSECA BLOISE, ALINE CORDEIRO, ALESSANDRA GRANATO, ALBERTO FÉLIX NÓBREGA, CARMEN CABANELAS PAZOSMOURA, VALÉRIA DE MELLO-COELHO H - 12 PS20 AFFECTS UPA SECRETION AND THE BEHAVIOR OF ENDOTHELIAL CELLS SILVIA BORGES PIMENTEL DE OLIVEIRA, HERNANDES F CARVALHO H - 13 EVALUATION OF THE NEUROTOXIC/NEUROPROTECTIVE ROLE OF ORGANOSELENIDES USING DIFFERENTIATED HUMAN NEUROBLASTOMA SH-SY5Y CELL LINE CHALLENGED WITH 6-HYDROXYDOPAMINE LEONARDO LISBOA DA MOTTA, FERNANDA MARTINS LOPES, GIOVANA FERREIRA LONDERO, LIANA MARENGO DE MEDEIRO, GUILHERME ANTONIO BEHR, VALESKA AGUIAR DE OLIVEIRA, MOHAMED IBRAHIM, JOSÉ CLÁUDIO FONSECA MOREIRA, LISIANE DE OLIVEIRA PORCIÚNCULA, JOÃO BATISTA TEXEIRA DA ROCHA, FÁBIO KLAMT H - 14 ROLE OF STROTIUM RANELATE ON PROLIFERATION AND OTEOGENIC DIFFERENTIATION OF HUMAN MESENCHYMAL STROMAL CELLS RHAYRA BRAGA DIAS, DANIELLE C. BONFIM, RADOVAN BOROJEVIC, MARCOS FARINA, MARIA ISABEL DORIA ROSI H - 15 ROS INDUCES DIFFERENTIATION OF NORMAL AND LEUKEMIC HEMATOPOIETIC CELLS AMANDA NOGUEIRA PEDRO, THALYTA APARECIDA CESÁRIO MUNHOZ, CHRISTIANO MARCELLO VAZ BARBOSA, CAROLINA CARVALHO DIAS, EDGAR JULIAN PAREDES-GAMERO, ALICE TEIXEIRA FERREIRA H - 16 ESTABLISHMENT OF HEMOBLAST CULTURE FROM THE HEMATOPOIETIC SITE OF THE SEA SQUIRT STYELA PLICATA ISADORA SANTOS DE ABREU, SILVANA ALLODI, CÍNTIA MONTEIRO DE BARROS H - 17 BOVINE TENDON EXTRACT SUPPORTS THE DIFFERENTIATION OF ADULT HUMAN MESENCHYMAL STEM CELLS INTO TENOCYTE-LIKE CELLS. LÍVIA MARIA MENDONÇA AUGUSTO, F. A. MENDES, M. I. D. ROSSI, A. S. BALDUINO, P. L. CASADO, A. S. CAVALCANTE, V. F. VIANNA, D. C. BONFIM, J. F. M. BARCELLOS, M. E. L. DUARTE H - 18 EFFECT OF POLYAMINES ON THE CELLULAR MORPHOLOGY OF YARROWIA LIPOLYTICA ANTONIO JESUS DORIGHETTO COGO, ARNOLDO ROCHA FAÇANHA, ANNA LVOVNA OKOROKOVA FAÇANHA H - 19 IN VITRO OSTEOBLASTIC DIFFERENTIATION ON BIOACTIVE GLASSBASED MATERIALS GABRIELA CAROLINE ALONSO, OLÍVIA CHERUBIN ALVES, FABÍOLA SINGARETTI DE OLIVEIRA, ROGER RODRIGO FERNANDES, OSCAR PEITL, EDGAR DUTRA ZANOTTO, MÁRCIO MATEUS BELOTI, ADALBERTO LUIZ ROSA, PAULO TAMBASCO DE OLIVEIRA I – Cell Migration I1-I13 I-1 MORPHOLOGICAL AND BIOCHEMICAL ASPECTS OF A SUBACUTE LESION IN THE PROTOCEREBRAL TRACT OF THE CRAB UCIDES CORDATUS CLYNTON LOURENÇO CORREA, PAULA CHAVES DA SILVA, SILVANA ALLODI I-2 PLASMIN AND UROKINASE PROMOTES CELL MIGRATION VIA MEK/ERK CASCADE BRUNO ROCHA CORDEIRO COSTA, ALINE ALVES FORTUNATO DO CARMO, LEONARDO CAMILO DE OLIVEIRA, CAMILA RODRIGUES CHAVES NOGUEIRA, JULIANA PRISCILA VAGO DA SILVA, LUIZA OLIVEIRA PERUCCI, BRUNO DOS SANTOS ALVES FIQUEIREDO BRASIL, CLÁUDIO ANTÔNIO BONJARDIM, MAURO MARTINS TEIXEIRA, LIRLÂNDIA PIRES DE SOUSA I-3 EVALUATION OF RECOMBINANT CXCL12(5-67) CHEMOTACTIC ACTIVITY ON CXCR4+ CELLS IN VITRO TAÍS ADELITA DE ALMEIDA BARROS, ROBERTA SESSA STILHANO, SANG WON HAN, GISELLE ZENKER JUSTO, MARIMÉLIA PORCIONATTO I-4 CHONDROITIN SULFATE IMPAIRS NEURAL STEM CELL MIGRATION IN VITRO LAYLA TESTA GALINDO, PIERO BAGNARESI, MARINILCE FAGUNDES DOS SANTOS, MARIMÉLIA APARECIDA PORCIONATTO I-5 HEAT STRESS INFLUENCES ON IMMUNE PARAMETERS IN TROPICAL SEA URCHIN LYTECHINUS VARIEGATUS PAOLA CRISTINA BRANCO, MAÍRA ESTANISLAU SOARES DE ALMEIDA, RENATA STECCA IUNES, MARINILCE FAGUNDES DOS SANTOS, JOÃO CARLOS SHIMADA BORGES, JOSÉ ROBERTO MACHADO CUNHA DA SILVA I-6 IMMUNE SYSTEM CELLS OF THE RAT VENTRAL PROSTATE QUANTIFICATION BY HEALTHY, CASTRATED AND ESTROGENIZED ANIMALS JULIETE APARECIDA FRANCISCO DA SILVA, DAGMAR RUTH STACH-MACHADO, HERNANDES FAUSTINO DE CARVALHO I-7 THE ENDOPLASMIC RETICULUM CHAPERONE PROTEIN DISULFIDE ISOMERASE (PDI) IS REQUIRED FOR PDGF-INDUCED RHOGTPASE ACTIVATION AND NOX1 NADPH OXIDASE-DEPENDENT VASCULAR SMOOTH MUSCLE CELL MIGRATION LUCIANA PESCATORE ALVES, DIEGO BONATTO, FABIO LUIS FORTI, AMINE SADOK, HERVÉ KOVACIC, FRANCISCO RAFAEL MARTINS LAURINDO I-8 LTB4 AS CHEMOATTRACTANT FACTOR IN THE REGULATORY T CELLS MIGRATION CYNTIA PECLI E SILVA, RAPHAEL MOLINARO, MARC PETERSGOLDEN, STEVEN L. KUNKEL, CLAUDIO CANETTI, CLAUDIA FARIAS BENJAMIM I-9 HYPERGLYCEMIA IMPAIRS 2-D AND 3-D MIGRATION OF SKIN FIBROBLASTS, ALSO AFFECTING CELL ADHESION AND INTEGRINS SURFACE DISTRIBUTION MAÍRA ESTANISLAU SOARES DE ALMEIDA, KELLY SALZMANN MONTEIRO, SANDRA COCCUZZO SAMPAIO VERSSONI, TÁRCIO TEODORO BRAGA, NIELS OLSEN SARAIVA CÂMARA, MARCELO LAZZARON LAMERS, MARINILCE FAGUNDES DOS SANTOS I - 10 CHARACTERIZATION OF THE IMPAIRED WOUND HEALING IN THE DERMIS OF DIABETIC MICE JULIANA DA COSTA FLORIM, ANA FLÁVIA MARÇAL PESSOA, KAIO FERNANDO VITZEL, HOSANA GOMES RODRIGUES, RUI CURI, MARCELO LAZZARON LAMERS, MARINILCE FAGUNDES DOS SANTOS I - 11 ROLE OF LAMININ IN ALLOREACTIVE T-CELL MIGRATION DURING ACUTE REJECTION ARIANY OLIVEIRA SANTOS, WILSON SAVINO, INGO RIEDERER I - 12 IMMATURE THYMOCYTES ARE RELEASED INTO THE PERIPHERY OF TRYPANOSOMA CRUZI ACUTELY INFECTED MICE BY A S1P-DEPENDENT MECHANISM AILIN LEPLETIER, LILIANE SILVA DE ALMEIDA, NAIARA MARAN, MARCELO EINICKER LAMA, ANA ROSA PÉREZ, ALIRIO MELENDES, WILSON SAVINO, ALEXANDRE MORROT LIMA I - 13 IMMUNOENDOCRINE INTERACTIONS DURING LYMPHOCYTE MIGRATION IN HUMAN CHAGAS DISEASE LUIZ RICARDO BERBERT, ANA ROSA PÉREZ, OSCAR BOTTASSO, WILSON SAVINO J – Cell Signaling J1-J48 J-1 OUTSIDE-IN SIGNALING BY VE-CADHERIN PROMOTES LOCAL RAC ACTIVATION AND ENHANCEMENT OF LUNG ENDOTHELIAL BARRIER BY ILOPROST XINYONG TIAN, OLEKSII DUBROVSKYI, YUFENG TIAN, NOUREDDINE ZEBDA, NICOLENE SARICH, ANNA BIRUKOVA J-2 ASSOCIATION BETWEEN ADHERENS JUNCTIONS AND TIGHT JUNCTIONS VIA RAP1-AFADIN IS REQUIRED FOR PROTECTIVE EFFECTS OF OXIDIZED PHOSPHOLIPIDS NOUREDDINE ZEBDA, PANFENG FU, VALERY POROYKO, IVAN COKIC, KONSTANTIN BIRUKOV J-3 PROTEOMIC ANALYSIS OF SNAKE VENOM GLAND ACTIVATION AND VENOM PRODUCTION MILENE SCHMIDT LUNA, RICHARD HEMMI VALENTE, JONAS PERALES, MONICA LARUCCI VIEIRA, NORMA YAMANOUYE J-4 KNOCKDOWN OF ARHGAP21 MODULATES THE EXPRESSION OF GENES RELATED TO HYPOXIA AND GLYCOLYSIS IN ADENOCARCINOMA PROSTATE CELL LINES MARIANA LAZARINI, MARCOS MARANGONI, JOÃO AGOSTINHO MACHADO NETO, PATRICIA SEVEVERINO, CARLOS ALBERTO MOREIRA-FILHO, FABÍOLA TRAINA, SARA TERESINHA OLALLA SAAD J-5 SUB-CELLULAR LOCALIZATION OF NEK7 AND ITS INTERACTION PROTEINS EDMÁRCIA ELISA DE SOUZA, GABRIELA VAZ MEIRELLES, BÁRBARA BIATRIZ GODOY, EDUARDO CRUZ MORAES, JULIANA HELENA COSTA SMETANA, JÖRG KOBARG J-6 FLOTILLINS STABILIZE CADHERIN COMPLEXES AT CELL-CELL CONTACTS THROUGH INTERACTION WITH THE F-ACTIN CYTOSKELETON EMILIE GUILLAUME, FRANCK COMUNALE, STÉPHANE BODIN, CÉCILE GAUTHIER-ROUVIÈRE 108 J-7 SYMPATHETIC OUTFLOW ON PROTEIN EXPRESSION IN THE MOUSE PAROTID GLAND CÍNTIA SCUCUGLIA HELUANY, MILENE SCHIMIDT LUNA, NORMA YAMANOUYE J-8 FUNCTIONAL CHARACTERIZATION CELL CYCLE REGULATING KINASE NEK7 AND ITS INTERACTION PROTEINS BÁRBARA BIATRIZ DE GODOY, EDMÁRCIA ELISA SOUZA, SMETANA, J.H.C., JÖRG KOBARG J-9 INTEGRATION OF MULTIPLE SIGNALING ROUTES IN T CELLS CAN CONTROL GENERATION AND SURVIVAL OF LONG-LIVED ANTIBODY-SECRETING CELLS LIDIANE ZITO GRUND, MÔNICA LOPES FERREIRA, CARLA LIMA J - 10 PKC AND CALCIUM BUT NOT PLC ARE INTRACELLULAR MESSENGERS OF VASOCONSTRICTOR RESPONSE INDUCED BY ANGIOTENSIN II IN THE SNAKE CROTALUS DURISSUS TERRIFICUS MÉLANIE MRQ LE DIAGON, MARIA CRISTINA BRENO J - 11 CELLULAR DISTRIBUTION OF ADHESION MOLECULES (CAMS) IN PANCREATIC ISLETS OF OBESE AND PRE-DIABETIC MICE. VIVIANE TANNURI FERREIRA LIMA FALCAO, DANIELA APARECIDA MASCHIO, LUÍZA MARTINEZ PERDIGUEIRO, JUNIA CAROLINA REBELO DOS SANTOS SILVA, MARIANNE R SANTOS, CAROLINA P.F. CARVALHO, MARIA TEREZA CARTAXO, CARLA BEATRIZ COLLARES BUZATO J - 12 SPATIAL REGULATION OF RAS SIGNALLING NETWORKS VERONICA ARAN, IAN PRIOR J - 13 FLUOXETINE ATTENUATED TREK-2-MEDIATED APOPTOTIC CELL DEATH IN HEK293A CELLS KEE RYEON KANG, CHEOL SOON LEE J - 14 BRAIN CAMP/CA2+ SIGNALING GENES IN FAT TISSUE IMPLANTED POLYCYSTIC OVARIAN SYNDROME MOUSE JORGE KEDE, EDUARDO HENRIQUE DA SILVA FREITAS, CARLOS FERNANDES BATISTA, MARIA DE NAZARETH GAMBOA RITTO, ISIDORO BINDA NETO, SAMUEL MARCOS RIBEIRO DE NORONHA, SILVANA APARECIDA ALVES CORRÊA DE NORONHA, ISMAEL DALE COTRIM GUEREIRO DA SILVA J - 15 TYROSINE PHOSPHORYLATION PLAYS A ROLE IN INCREASING MASPIN PROTEIN LEVELS AND ITS CYTOPLASMIC ACCUMULATION MARIANA TAMAZATO LONGHI, NATHALIE CELLA J - 16 NADPH OXIDASE ACTIVATION MEDIATED BY PROTEIN DISULFIDE ISOMERASE OVEREXPRESSION IN VSMC: ROLE OF ANGIOTENSIN II RECEPTOR (AT1R) RENATA DE CASTRO GONÇALVES, FRANCISCO RAFAEL MARTINS LAURINDO, DENISE DE CASTRO FERNANDES J - 17 TXNIP IS RESPONSIBLE FOR IMPAIRED GLUCOSE TOLERANCE IN THE DIABETIC MICE JO SEONG-HO, PARK JOO-MAN, KIM MI-YOUNG, KIM TAEHYUN, AHN YONG-HO J - 18 PALMITATE INDUCES INTRACELLULAR CALCIUM (CA2+) MOBILIZATION IN MONONUCLEAR CELLS FROM TYPE 2 DIABETIC PATIENTS CAROLINE MARIA DE OLIVEIRA VOLPE, FERNANDA SARMENTO FAGUNDES-NETTO, RAQUEL MIRANDA GONZAGA, POLLYANNA STEPHANIE GOMES, JOSÉ AUGUSTO NOGUEIRA-MACHADO J - 19 TIMP1/ Β1-INTEGRIN/CD63 COMPLEX: RESISTANCE TO ANOIKIS ALONG MELANOCYTE MALIGNANT TRANSFORMATION BY PI3-K/AKT SIGNALING PATHWAY MARIANA TORICELLI PINTO, FABIANA HENRIQUES MACHADO DE MELO, MIRIAM GALVONAS JASIULIONIS J - 20 ACTIVATION OF RAC1 THROUGH A FAK-INDEPENDENT PATHWAY IN INHIBITION OF MIGRATION MEDIATED BY RECK GENE IN HUMAN GLIOMA RAQUEL BRANDÃO HAGA, FERNANDA LEVE, JOSÉ ANDRÉ MORGADO-DIAZ, SILVYA STUCHI MARIA-ENGLER J - 21 SUMOYLATION OF THE HUMAN REGULATORY PROTEIN KI-1/57 AND ITS FUNCTION IN THE CELL ÂNGELA SAITO, KALIANDRA DE ALMEIDA GONÇALVES, MARCOS TADEU DOS SANTOS, JÖRG KOBARG RODRIGUES, FABIO DUPART NASCIMENTO, EDGAR JEAN PAREDES GAMERO, IVARNE LUIS DOS SANTOS TERSARIOL, HELENA BONCIANI NADER J - 27 CHARACTERIZATION OF MASPIN SUMOYLATION CRISTIANE LUMI HIRATA, NATHALIE CELLA J - 28 PROTEIN KINASE B (PKB, AKT) IS INVOLVED IN RHIPICEPLAHUS (BOOPHILUS) MICROPLUS EMBRYO CELL LINE BME26 SURVIVAL LEONARDO ARAUJO DE ABREU, CHRISTIANO CALIXTO DA CONCEIÇÃO, SATORU KONNAI, KASUHIRO OHASHI, ITABAJARA DA SILVA VAZ JR, CARLOS JORGE LOGULLO DE OLIVEIRA J - 29 A POSSIBLE INTERPLAY BETWEEN RELAXIN AND FSH TO REGULATE SERTOLI CELL FUNCTION. ALINE ROSA DO NASCIMENTO, THAÍS FABIANA GAMEIRO LUCAS, CATARINA SEGRETI PORTO, MARIA FÁTIMA MAGALHÃES LAZARI J - 30 PS-1/GAMMA-SECRETASE-DEPENDENT CADHERIN CLEAVAGE REGULATES OSTEOGENIC DIFFERENTIATION OF HUMAN BONE MARROW MESENCHYMAL STROMAL CELLS BY CONTROLLING THE TRANSLOCATION OF THE ACTIVE SIGNALING FORM OF BETA-CATENIN TO NUCLEUS DANIELLE CABRAL BONFIM, RHAYRA BRAGA DIAS, CLAUDIA DOS SANTOS MERMELSTEIN, MARIA ISABEL DORIA ROSSI J - 31 NITRIC OXIDE AMELIORATES THE OXIDATIVE STRESS INDUCED BY ARSENIC IN WATER HYACINTH HELOÍSA MONTEIRO DE ANDRADE, JURACI ALVES DE OLIVEIRA, JOSÉ LINO NETO, JOSÉ CAMBRAIA, FERNANDA DOS SANTOS FARNESE, CRISTIANE JOVELINA DA SILVA J - 32 DOES NITRIC OXIDE ACTIVATE ANTIOXIDATIVE ENZYMES IN PLANTS EXPOSED TO ARSENIC? JURACI ALVES DE OLIVEIRA, HELOÍSA MONTEIRO DE ANDRADE, JOSÉ LINO NETO, JOSÉ CAMBRAIA, FERNANDA DOS SANTOS FARNESE J - 33 MODULATION OF NADPH OXIDASE BY PROTEOGLYCANS IN CHOK1 AND CHO-745 CELLS. SHEYLA VARELA LUCENA, GISELLE ZENKER JUSTO, ZAIANE MENESES CAMILO, TIAGO RODRIGUES, DAYSE CAROLINE SEVERIANO DA CUNHA, HELENA BONCIANI NADER, IVARNE LUIS DOS SANTOS TERSARIOL J - 34 CHARACTERIZATION OF EPITHELIAL CELL SIGNALLING DURING CANDIDA ALBICANS AND NON-ALBICANS INVASION BY INDUCED ENDOCYTOSIS DIANA BAHIA, ALEXIS BONFIM-MELO, PALOMA KOREHISA MAZA, RENATO ARRUDA MORTARA, ARNALDO COLOMBO, ANA CAROLINA BARBOSA PADOVAN, ERIKA SUZUKI, RICARDO SÉRGIO COUTO DE ALMEIDA J - 35 SIGNALING PATHWAYS ASSOCIATED WITH NEUTROPHIL EXTRACELLULAR TRAPS (NETS) FORMATION BY NEUTROPHILS STIMULATED WITH LEISHMANIA AMAZONENSIS THIAGO SOARES DE SOUZA VIEIRA, ANDERSON GUIMARÃES BAPTISTA COSTA, MICHELLE TANNY CUNHA DO NASCIMENTO, RAFAEL MARIANTE, ELVIRA MARIA SARAIVA J - 36 EXPRESSION PROFILE ASSESSMENT OF NUCLEAR RECEPTORS AND CO-REGULATORS GENES IN BREAST CANCER CELL SK-BR3 AFTER HCG, ANGIOTENSIN 1-7 AND ESTRADIOL TREATMENTS ISIDORO BINDA NETO, WERICA BERNARDO, GABRIELA SOARES BRITO, ADRIANA CARVALHO, JORGE KEDE, EDUARDO HENRIQUE DA SILVA FREITAS, CARLOS FERNANDES BATISTA, MARIA DE NAZARETH GAMBOA RITTO, SAMUEL MARCOS RIBEIRO DE NORONHA, SILVANA APARECIDA ALVES CORRÊA DE NORONHA, GIL FACINA, ISMAEL DALE COTRIM GUERREIRO DA SILVA J - 37 COMPARTMENTALIZATION OF REDOX PROCESSES, NOX4 AND ENDOPLASMIC RETICULUM CHAPERONES WITHIN LIPID DROPLETS IN VASCULAR SMOOTH MUSCLE CELLS. THALITA BALSAMO ABRAHAO, ANGELICA A AMANSO, VICTOR DEBBAS, PATRICIA T BOZZA, EDLAINE LINARES, OHARA AUGUSTO, BERNARD LASSEGUE, KATHY K GRIENDLING, FRANCISCO RM LAURINDO J - 38 IS TRYPANOSOMA CRUZI P21 A CHEMOKINE-LIKE PROTEIN? ADELE AUD RODRIGUES, TATIANA MORDENTE CLEMENTE1, FABRÍCIO CASTRO MACHADO, PAULO CÉSAR FERREIRA DOS SANTOS, FERNANDO DE QUEIRÓZ CUNHA, TIAGO WILSON PATRIARCA MINEO, CLAUDIO VIEIRA DA SILVA J - 22 REGULATION OF WILSON DISEASE ATPASE (ATP7B) ACTIVITY LUIZA HELENA DALTRO CARDOSO, ELAINE HILARIO DE SOUZA, ADALBERTO VIEYRA, JENNIFER LOWE J - 39 EARLY WEANING-STIMULATED PROLIFERATION AND DIFFERENTIATION IN THE GASTRIC MUCOSA: ARE MAPK OR SRC SIGNALING PATHWAYS INVOLVED? LUCIANA HARUMI OSAKI, CRUZ ALBERTO MENDOZA RIGONATI, PATRÍCIA GAMA J - 23 MODULATION OF TRL2 AND TLR4 ACTIVATION BY MAPK INHIBITOR :EVALUATION OF ROS PRODUCTION BY PBMNC FORM TYP2 DIABETIC PATIENTS. FERNANDA SARMENTO FAGUNDES NETTO, CAROLINE MARIA OLIVEIRA VOLPE, RAQUEL MIRANDA GONZAGA, POLLYANNA STEPHANIE GOMES, JOSÉ AUGUSTO NOGUEIRA-MACHADO J - 40 THIOREDOXIN-1 INTERACTS WITH ADAM17 AND MODULATES ITS HB-EGF SHEDDASE IN A P38 AND ERK1/2-INDEPENDENT PATHWAYS ANNELIZE Z B ARAGAO, DANIELA C GRANATO, MARIA LUIZA C NOGUEIRA, FERNANDO M SIMABUCO, ANA C M ZERI, ADRIANA F PAES LEME J - 24 TRANSITION TO WIDESPREAD VASCULAR CELL LOSS DUE TO SUSTAINED ENDOPLASMIC RETICULUM STRESS IS MARKED BY INCREASED OXIDANT GENERATION, BUT OXIDANTS DO NOT INDUCE CELL DEATH JOÃO WOSNIAK JÚNIOR, PHELIPE MONTEIRO FELÍCIO, NATHÁLIA ARAÚJO, FRANCISCO RAFAEL MARTINS LAURINDO J - 25 FGFS/FGFRS SIGNALING IN HUMAN KERATINOCYTES EDUARDO LOPES DA SILVA, JULIANA DIAS ZEIDLER, ANDRE ZELANI, SOLANGE M T SERRANO, HUGO AGUIRRE ARMELIN J - 26 MODULATION OF P2X7 RECEPTORS BY GLYCOSAMINOGLYCANS (GAG) GIOCONDA EMANUELLA DINIZ DE DANTAS MOURA, RAFAEL LIMA CASAES J - 41 INVOLVEMENT OF PROTEIN KINASE C AND PHOSPHOLIPASE C IN THE ACTIVATION OF CLOCK GENES BY BLUE LIGHT MARIA NATHÁLIA DE CARVALHO MAGALHÃES MORAES, BRUNO C. R. RAMOS, LEONARDO HENRIQUE R G LIMA, ANA MARIA DE LAURO CASTRUCCI J - 42 ACTIVATION OF THE ASCORBATE-GLUTATHIONE CYCLE BY NITRIC OXIDE: SIGNALING UNDER STRESS CONDITIONS TRIGGERED BY ARSENIC FERNANDA SANTOS FARNESE, JURACI ALVES DE OLIVEIRA, LUHAN ISAAC SIMAN, NEIDIQUELE MARIA DA SILVEIRA, GRASIELLE SOARES GUSMAN J - 43 RESTORATION OF LEPTIN RECEPTORS IN POMC NEURONS IN DB/DB MICE AND ROLE OF LIVER IN MAINTAINING THE GLUCOSE HOMEOSTASIS 109 MARIANA SARTO FIGUEIREDO, ALAN VICENTE FERREIRA, PATRICIA CRISTINA LISBOA, ELAINE DE OLIVEIRA, CHRISTIAN BJORBAEK, EGBERTO GASPAR DE MOURA ALMEIDA, ANA LUCIA ABUJAMRA, ALGEMIR LUNARDI BRUNETTO, GILBERTO SCHWARTSMANN, RAFAEL ROESLER J - 44 INVESTIGATION OF THE MECHANISM OF MOLECULAR INTERACTION BETWEEN THE N-TYPE CALCIUM CHANNELS AND G PROTEINS LUCIENE BRUNO VIEIRA, LUARA AUGUSTA BATISTA, MARCUS VINÍCIUS GOMEZ, GERALD WERNER ZAMPONI K - 11 EVALUATION OF THE WHOLE BONE MARROW INFLUENCE ON THE INNATE IMMUNITY IN A MODEL OF ACUTE LIVER FAILURE CAROLINA URIBE CRUZ, CARLOS OSCAR KIELING, MÓNICA LUJÁN LÓPEZ, LAURA SIMON, GUSTAVO OCH MUÑOZ, LUISE MEURER, URSULA MATTE J - 45 LIMITED PROTEOLYSIS BY CALPAIN A REGULATES CACTUS/IKAPPAB FUNCTION AND DORSAL-VENTRAL PATTERNING IN THE DROSOPHILA EMBRYO HELENA ARAUJO, MARCIO FONTENELE, GERTHRUD SCHUPBACH K - 12 PLATELET IMPROVES SURVIVAL IN A RAT MODEL OF ACUTE LIVER FAILURE LAURA SIMON, MÓNICA LUJAN LÓPEZ, ALESSANDRO OSVALDT, CAROLINA URIBE CRUZ, CARLOS OSCAR KIELING, GUSTAVO OCHS DE MUÑOZ, LUISE MEURER, URSULA MATTE J - 46 NOVEL MODULATORS OF WNT/BETA-CATENIN PATHWAY THROUGH FUNCTIONAL SCREENING OF NATURAL COMPOUNDS BÁRBARA DE FARIA DA FONSECA, DÉBORA MALTA CERQUEIRA, NATHÁLIA DA GRAÇA AMADO, RICARDO KUSTER, JOSÉ GARCIA ABREU JR K - 13 CELL THERAPY FOR ACUTE LIVER FAILURE: EXPERIMENTAL STUDY WITH MICROENCAPSULATED CELLS LAURA SIMON, CARLOS OSCAR KIELING, CAROLINA URIBE CRUZ, MÓNICA LUJAN LÓPEZ, ALESSANDRO OSVALDT, GUSTAVO OCHS DE MUÑOZ, LUISE MEURER, URSULA MATTE J - 47 P2X7 RECEPTOR MEDIATES APOPTOSIS IN EPITHELIAL CELLS HCT8 VIA REACTIVE OXYGEN SPECIES VANESSA RIBEIRO FIGLIUOLO, HAYANDRA FERREIRA NANINI, ALESSANDRA ALVES ABALO, GIANI FRANÇA SANTORO, CLÁUDIA MARA LARA MELO COUTINHO, ROBSON COUTINHO SILVA K - 14 PRODUCTION OF RECOMBINANT HUMAN VASCULAR ENDOTHELIAL GROWTH FACTORS IN MAMMALIAN EXPRESSION SYSTEMS FOR ANGIOGENESIS AND WOUND HEALING ANA CLAUDIA OLIVEIRA CARREIRA, GUSTAVO GROSS BELCHIOR, FERNANDA CÂMARA MARQUES SODRÉ, THERI LEICA THEGAKI, RAQUEL SANTANA CRUZ, MARI CLEIDE SOGAYAR J - 48 MESENTERIAL FAT UPR AND TLR4 ACTIVATION MARKERS WERE ASSOCIATED WITH OBESOGENIC GUT MICROBIOTA CARLA EVELYN COIMBRA NUÑEZ, VIVIANE SOARES RODRIGUES, MURILLO LINO BUTION, RAFAEL DE MORAES PEDRO, MÁRCIO JOSÉ DA SILVA, ÉRIKA ANNE ROBLES ROMAN, DANIELE CRISTINA VITORINO, WANDERLEY DIAS DA SILVEIRA, ELIANA PEREIRA DE ARAÚJO K – Cell Therapy K - 15 THE PRE-CONDITIONING WITH BRADYKININ-POTENTIATING PEPTIDES DERIVED FROM BOTHROPS JARARACA SNAKE VENOM INCREASES THE THERAPEUTIC EFFECT OF ANGIOGENIC STEM CELLS IN A HINDLIMB ISCHEMIA MODEL ALAN SALES BARBOSA, BRÍGIDA GOMES DE ALMEIDA SCHIMER, MARIA CECÍLIA CAMPOS CANESSO, JOUSIE MICHEL PEREIRA, DANIELLE ALVES IANZER, ANTÔNIO CARLOS MARTINS DE CAMARGO, PATRÍCIA GONÇALVES TEIXEIRA, ANTÔNIO CARLOS VIEIRA CABRAL, MAURO MARTINS TEIXEIRA, LUCÍOLA DA SILVA BARCELOS K1-K16 K - 16 BONE MARROW MONONUCLEAR CELL THERAPY REDUCES REACTIVE MICROGLIOSIS AND HIPPOCAMPAL CA1 PYRAMIDAL CELL DEATH FOLLOWING GLOBAL CEREBRAL ISCHEMIA IN RATS. ALANE BERNARDO RAMOS, ANDRÉIA VASCONCELOS- DOS-SANTOS, WAGNER MONTEIRO CINTRA, ROSALIA MENDEZ-OTERO K-1 HEPATIC CELLS: AN ALTERNATIVE SOURCE TO REPOPULATE THE BIO-ARTIFICIAL LIVER LANUZA ALABY PINHEIRO FACCIOLI, GRAZIELLE SUHETT DIAS, LUIZ FERNANDO QUINTANILHA, SANDRO TORRENTES CUNHA, BERNARDO JORGE DA SILVA MENDES, RACHEL RACHID, MARCIA ATTIAS, CHRISTINA MAEDA TAKIYA, ADRIANA BASTOS CARVALHO, REGINA COELI DOS SANTOS GOLDENBERG L – Cells as Biosensors K-2 BONE MARROW CELLS DERIVED-FIBROBLAST GROWTH FACTOR-2 INDUCES GLIAL CELL PROLIFERATION IN THE REGENERATING PERIPHERAL NERVOUS SYSTEM ALVARO CARRIER RUIZ, ROBERTHA MARIANA RODRIGUES LEMES, RICARDO AUGUSTO DE MELO REIS, ROSALIA MENDEZ-OTERO, VICTOR TÚLIO RIBEIRO RESENDE K-3 IDENTIFICATION OF CARDIOMYOCYTES TRANSDIFFERENTIATED FROM ADIPOSE DERIVED MESENCHYMAL STEM CELLS BIANCA FERRARINI ZANETTI, SANG WON HAN K-4 MONONUCLEAR CELLS FROM HUMAN UMBILICAL CORD BLOOD PROMOTES FUNCTIONAL RECOVERY AFTER SPINAL CORD INJURY IN RATS LUCIANO PALMEIRO RODRIGUES, DAIKELLY IGLESIAS BRAGHIROLLI, FABRÍCIO DO COUTO NICOLA, DANIELA STEFFENS, LAUREN VALENTIM, ALESSANDRO WITCZAK, GEANCARLO ZANATTA, MATILDE ACHAVAL, PATRICIA PRANKE, CARLOS ALEXANDRE NETTO K-5 RETINAL SPHERES SIMILAR STRUCUTURES-DERIVED FROM DENTAL PULP STEM CELLS ARE POSITIVE FOR PAX-6 MARKER BRUNA PEREIRA DE MORAIS, LISLEY INATA MAMBELLI, NELSON FORESTO LIZIER, BABYLA GERALDES MONTEIRO, JOSÉ ÁLVARO PEREIRA GOMES, IRINA KERKIS K-6 INTERNALIZATION ASSAY OF POLY-Ε-CAPROLACTONE NANOPARTICLES USING PERITONEAL MACROPHAGES: THE ACTION OF FREE OR NANOENCAPSULATED ZINC (II) PHTALOCYANINE AMANDA SANTOS FRANCO DA SILVA, EDUARDO RICCI JUNIOR, MORGANA TEIXEIRA LIMA CASTELO BRANCO, LYCIA DE BRITO GITIRANA K-7 INSULIN COATING IN HIDROXYAPATITE INCREASES OSTEOBLASTIC CELLS ATTACHMENT MOEMA A HAUSEN, ELENA MAVROPOULOS, JÉSSICA DORNELAS, SUZANA DOS ANJOS, ALEXANDRE ROSSI K-8 RADIATION-INDUCED LIVER DAMAGE IN MICE: AN EXPERIMENTAL MODEL TO STUDY TISSUE REGENERATION GRAZIELLESUHETT DIAS, LANUZA ALABY FACCIOLI PINHEIRO, SANDRO TORRENTES CUNHA, TIAGO SOUZA VILASBÔAS, ALYNE HENRIQUES CORDEIRO, LUIZ FERNANDO QUINTANILHA, BRUNO DIAZ PAREDES, CRISTINA MAEDA TAKIYA, PAULO CÉSAR CANARY, ADRIANA BASTOS CARVALHO, REGINA COELI GOLDENBERG SANTOS L1-L7 L-1 ENVIRONMENTAL ASSESSMENT USING MUCOUS CELLS OF SCALE EPITHELIUM IN FISH REBECA MAMEDE DA SILVA ALVES, JOSÉ AUGUSTO SENHORINI, RITA DE CÁSSIA GIMENEZ DE ALCÂNTARA ROCHA, BRUNO FIORELINI PEREIRA, FLÁVIO HENRIQUE CAETANO L-2 CYTOMORPHOLOGICAL CHANGES IN THE ARMORED CATFISH LORICARICHTHYSANUS, IN THE SINOS RIVER (RIO GRANDE DO SUL, BRAZIL), DURING AN EPISODE OF FISH MORTALITY AND CORRELATION WITH THE PRESENCE OF HERBICIDES CARLOS AUGUSTO BORBA MEYER NORMANN, KARINA JOB DI LACCIO, MILENE SANTANA PINTO, VALESCA VEIGA CARDOSO CASALI L-3 ABCB1 AND ABCC1 PROTEINS ACTIVITY IN COELOMOCYTES OF THE SEA URCHIN ECHINOMETRA LUCUNTER. LEONARDO LIMA DOS SANTOS, PATRICIA MIRELLA DA SILVA SCARDUA, LUIS FERNANDO MARQUES DOS SANTOS L-4 EFFECTS OF CHRONIC USE OF GRAPE JUICE ON HEPATOCYTES OF WISTAR RATS IN HIGH-FAT DIET CARLOS AUGUSTO BORBA MEYER NORMANN, ISELDE BUCHNER, NIARA MEDEIROS, DENISE LACERDA, PAULA RIGON, JOÃO ANTÔNIO HENRIQUES, ROSANE GOMEZ, CAROLINE DANI, CLAUDIA FUNCHAL L-5 BIOMARKERS OF LEPROSY: NEUTROPHIL CD64 EXPRESSION AS A BIOMARKER OF ERYTHEMA NODOSUM LEPROSUM VERÔNICA SCHMITZ PEREIRA, RHANA BERTO PRATA, SHEILA SANTOS BRANDÃO, MAYARA ABUD MENDES, ALICE MIRANDA, JOSÉ AUGUSTO NERY, EUZENIR NUNES SARNO L-6 IMMUNOFLUORESCENCE OF THE MUSHROOM BODIES OF STINGLESS BEES, SCAPTOTRIGONA POSTICA (LATREILLE, 1807), TREATED WITH IMIDACLOPRID BY INGESTION HELLEN MARIA SOARES, ROBERTA CORNÉLIO FERREIRA NOCELLI, CYNTHIA RENATA DE OLIVEIRA JACOB, OSMAR MALASPINA L-7 EVALUATION OF THE TOXICITY ON STINGLESS BEE SCAPTOTRIGONA POSTICA (HYMENOPTERA, APIDAE MELIPONINI) MUSHROOM BODIES, TREATED WITH SUBLETHAL DOSES OF FIPRONIL CYNTHIA RENATA DE OLIVEIRA JACOB, HELLEN MARIA SOARES, ROBERTA CORNÉLIO FERREIRA NOCELLI, OSMAR MALASPINA K-9 REPAIR OF SKIN DAMAGE WITH ADIPOSE-DERIVED STEM CELLS USING SODIUM CARBOXYMETHYLCELLULOSE SCAFFOLD IN RATS CRISTIANO RODRIGUES, ELISA VASCONCELLOS SOARES, ADRIANO MARTIMBIANCO DE ASSIS, MARILDA DA CRUZ FERNANDES, LÉDER LEAL XAVIER, ALEXANDRE TAVARES DUARTE DE OLIVEIRA, MÁRCIA ROSÂNGELA WINK K - 10 A GASTRIN-RELEASING PEPTIDE RECEPTOR ANTAGONIST ENHANCES VIABILITY AND PROLIFERATION OF NEUROBLASTOMA CELLS: PREVENTION BY A HISTONE DEACETYLASE INHIBITOR VIVIANE RÖSNER DE 110 M – Cytoskeleton M1-M13 M -1 VISUALIZATION OF CYTOSKELETON OF TRITRICHOMONAS FOETUS BY FESEM (HIGH RESOLUTION FIELD EMISSION SCANNING ELECTRON MICROSCOPY) IVONE ROSA DE ANDRADE, WANDERLEY DE SOUZA, MARLENE BENCHIMOL M -2 A SMALL MOLECULE TARGETING CDC42-INTERSECTIN INTERACTION DISRUPTS GOLGI ORGANIZATION AND SUPPRESSES CELL MOTILITY AMY FRIESLAND, YAXUE ZHAO, YAN-HUA CHEN, HUCHEN ZHOU, QUN LU M -3 THE IMMUNOLOCALIZATION OF CYTOSKELETAL PROTEINS AND THE BASEMENT MEMBRANE MARKER LAMININ IN THE OVIDUCT OF THE DOMESTIC FOWL (GALLUS DOMESTICUS) MARY CATHRINE MADEKUROZWA M -4 PROBABLE INVOLVEMENT OF THE RAC1 GTPASE IN RADIOSENSITIVITY OF HELA CELLS JULIANA HARUMI OSAKI, GISELE ESPINHA TEIXEIRA DA SILVA, FÁBIO LUIS FORTI M -5 RHOA GTPASE MEDIATES RECOVERY OF MELANOMA CELLS DAMAGED BY ULTRAVIOLET RADIATION GISELE ESPINHA TEIXEIRA DA SILVA, FÁBIO LUÍS FORTI M -6 PROTEIN INTERACTION DURING ASYMMETRIC CELL DIVISION IN BACILLUS SUBTILIS MAXWELL DE CASTRO DURVALE, FREDERICO JOSE GUEIROS FILHO M -7 REGULATION OF MICROTUBULES DYNAMICS BY DYNAMIN-2 IN HELA CELLS MAKIKO MORITA, KOZUE HAMAO, KEITA TANAKA, YASUYUKI SERA, HIROSHI HOSOYA M -8 PARTICIPATION OF CORTICAL ACTIN FROM NON- AND PHAGOCYTIC CELLS DURING TOXOPLASMA GONDII INVASION PROCESS BRENO COSTA LANDIM, SARA HISSAE HIRAIWA, PRISCILA SILVA FRANCO, ELOISA VIEIRA AMÁLIA FERRO, JULIANA GONZAGA DE OLIVEIRA M -9 CYTOKERATINS CROSSTALK IN BREAST CANCER THAISE GONÇALVES DE ARAÚJO, KARINA MARANGONI, YARA CRISTINA DE PAIVA MAIA, GALBER RODRIGUES ARAÚJO, PATRÍCIA TERRA ALVES, LARISSA PRADO MAIA, DONIZETE WILLIAM SANTOS, LUANDA CALÁBRIA, CARLOS UEIRA-VIEIRA, LUIZ RICARDO GOULART FILHO M -10 STUDYING A PROTEIN-PROTEIN INTERACTION THAT REGULATES BACTERIAL CELL DIVISION VALDIR BLASIOS JUNIOR, ALEXANDRE WILSON BISSON FILHO, PATRÍCIA CASTELLEN, RODRIGO PORTUGAL, JEFFERSON BETTINI, FREDERICO GUEIROS FILHO M -11 EXPRESSION OF THE CYTOSKELETAL PROTEINS ACTIN AND TUBULIN IN OSTEOGENIC CELLS CULTURED ON BIOACTIVE GLASS-BASED SURFACES CAROLINA SCANAVEZ MARTINS, LUCAS NOVAES TEIXEIRA, LARISSA MOREIRA SPINOLA DE CASTRO, MÁRCIO MATEUS BELOTI, ADALBERTO LUIZ ROSA, PAULO TAMBASCO DE OLIVEIRA M -12 MURINE MACROPHAGE CYTOSKELETON ALTERATIONS CAUSED BY AQUEOUS EXTRACT OBTAINED FROM ROOTS OF PHYSALIS ANGULATA RAQUEL RAICK PEREIRA DA SILVA, ANA PAULA DRUMMOND RODRIGUES, AMANDA ANASTÁCIA PINTO HAGE, GILMARA DE NAZARETH TAVARES BASTOS, EDILENE OLIVEIRA DA SILVA M -13 MITOCHONDRIA ARE ANCHORED BY ACTIN FILAMENTS TO THE CORTEX AND ARE MOTILE IN SEA URCHIN EGGS ISSEI MABUCHI, TOMOKO TAKAGI, SHINYA INOU, MAKOTO GODA N – Developmental Biology N1-N51 N-1 INFLUENCE OF SONIC HEDGEHOG SIGNALLING PATHWAY IN THE EAR DEVELOPMENT ALICE HELENA DOS REIS RIBEIRO, JOSÉ GARCIA RIBEIRO ABREU, JOSÉ MARQUES DE BRITO NETO N-2 ECTODERMAL-DERIVED ENDOTHELIN1 DRIVES DEVELOPMENT OF THE MOUSE MANDIBULAR ARCH INTERMEDIATE DOMAI ANDRE LUIZ PASQUA TAVARES, ELVIN L GARCIA, KATHERIN KUHN, CRYSTAL M WOODS, TREVOR WILLIAMS, DAVID E. CLOUTHIER EMBRYONIC DEVELOPMENT APARECIDA DAS DORES TEIXEIRA, MARIA DO CARMO QUEIROZ FIALHO, JOSÉ COLA ZANUNCIO, JOSÉ EDUARDO SERRÃO N-4 DEVELOPING A MODEL FOR THE STUDY OF THE HUMAN MESENCHYMAL STEM CELLS’ POTENCY USING CHICK EMBRYOS INGRID ROSENBURG CORDEIRO, JOSÉ MARQUES DE BRITO NETO, MARIA ISABEL DORIA ROSSI N-5 PIWI PROTEINS AND HISTONE METHYLATION IN THE RAT PRIMORDIAL GERM CELL DEVELOPMENT RENATO BORGES TESSER, TAIZA STUMPP N-6 RAT GONOCYTE QUIESCENCE DEPENDS ON CASPASE 3 EXPRESSION AND OCT4 DOWNREGULATION PRISCILA HENRIQUES DA SILVA, RENATO BORGES TESSER, CRISTIANE TOBARA MARUYAMA, TAIZA STUMPP N-7 UVB ENVIRONMENTALLY RELEVANT DOSES INHIBIT EARLY EMBRYONIC DEVELOPMENT OF SEA URCHIN ECHINOMETRA LUCUNTER. JOCELMO CÁSSIO DE ARAUJO LEITE, SUELENN GUEDES DA SILVA, JOSÉ PINTO DE SIQUEIRA JUNIOR, LUIS FERNANDO MARQUES DOS SANTOS N-8 ABCB1 PROTEIN PROTECTS SEA URCHIN EMBRYONIC DEVELOPMENT AGAINST UVB INJURIOUS EFFECTS. SUELLEN GUEDES DA SILVA, JOCELMO CÁSSIO DE ARAUJO LEITE, JOSÉ PINTO DE SIQUEIRA JUNIOR, LUIS FERNANDO MARQUES-SANTOS N-9 INVOLVEMENT OF MITOCHONDRIAL PERMEABILITY TRANSITION PORE (MPTP) AND CALCIUM RELEASING IN FERTILIZED EGGS OF SEA URCHIN. ELIS TORREZAN GONÇALVES RAMALHO NITÃO, LUIS FERNANDO MARQUESSANTOS N - 10 PRENATAL GLUCOCORTICOID TREATMENT AFFECTS ERYTHROID AND MEGAKARYOCYTIC CELLS OF RAT FETUSES AND NEWBORNS FLÁVIA MACEDO DE OLIVEIRA NEVES, CAMILA CICCONI PACCOLA, SANDRA MIRAGLIA, IVONE CIPRIANO N - 11 HDAC ACTIVITY IS NECESSARY FOR THE MORPHOGENESIS OF POLARIZED TISSUES CARLA AUGUSTA BARRETO MARQUES, ERIKA NEGREIROS, HELENA ARAUJO, KATIA CARNEIRO, TATHYANA LAMIM N - 12 CHARACTERIZATION OF ABCB1 AND ABCC1 PROTEINS FUNCTIONAL ACTIVITY DURING EMBRYONIC DEVELOPMENT OF SEA URCHIN ECHINOMETRA LUCUNTER ELIS TORREZAN GONÇALVES RAMALHO NITÃO, CAIO CEZAR OLIVEIRA DE LUCENA, LUIS FERNANDO MARQUES-SANTOS N - 13 THE SUBCELLULAR DISTRIBUTION OF THE ASYMMETRIC PROTEIN NODAL IN GLIAL CELLS PLAYS A KEY ROLE DURING DEVELOPMENT AND PHYSIOPATHOLOGY OF THE CENTRAL NERVOUS SYSTEM MARIA CECÍLIA OLIVEIRA FERREIRA NUNES, GUILHERME MARQUES DE MATTOS, SUZANA A. KAHN, FLAVIA LIMA, VIVALDO MOURA-NETO, KATIA CARNEIRO N - 14 EFFECTS OF TAURINE SUPPLEMENTATION ON ARTERIAL PRESSURE AND THE NEURONAL NUMBER OF BRAIN STEM IN THE OFFSPRING OF FEMALE RATS SUBJECTED TO PROTEIN RESTRICTION DURING PREGNANCY JOSE EDUARDO SCABORA, MARCELO CARDOSO DE LIMA, PATRÍCIA ALINE BOER, JOSE ANTOMIO ROCHA GONTIJO N - 15 RGMA EXPRESSION PATTERN IN SKELETAL MUSCLE CELLS IS SIMILAR TO MYOSINS ALINE FAGUNDES MARTINS, GREGORY THOMAS KITTEN, GERLUZA APARECIDA BORGES SILVA, DÉBORA CRISTINA INDELICATO DE MIRANDA, AMANDA VASCONCELOS ALBUQUERQUE, LUIZ LEHMANN COUTINHO, ÉRIKA CRISTINA JORGE N - 16 CHARACTERIZATION OF THE PATTERN OF BHC4-1-LACZ EXPRESSION IN DROSOPHILA LINES TRANSFORMED WITH MUTANT VERSIONS OF THE BHC4-1 RING GLAND ENHANCER IVANA MARIA DE ARAUJO FURTADO, MARÍLIA HARUMI ISHIZAWA, NADIA MONESI N - 17 MORPHOLOGICAL ASSESSMENT OF THE HEART OF THE MOSQUITO AEDES AEGYPTI DURING THE POST-EMBRYONIC DEVELOPMENT ANA CAROLINA MACHADO LEÓDIDO, GUSTAVO FERREIRA MARTINS N - 18 RATART RETROTRANSPOSON INTERFERING IN THE LARVAL DEVELOPMENT OF RHYNCHOSCIARA AMERICANA PAULA REZENDE-TEIXEIRA, GLAUCIA MARIA MACHADO-SANTELLI N - 19 THE ROLE OF THE MATERNAL DPP/BMP PATHWAY ON THE REGULATION OF RNA LEVELS IN THE EARLY DROSOPHILA MELANOGASTER EMBRYO. NATHÁLIA PENTAGNA MACIELLO DRUMMOND PIRES, MARCIO RIBEIRO FONTENELE, HELENA MARIA MARCOLLA ARAÚJO N - 20 DNA DAMAGE SIGNALING AND REPAIR PATHWAYS IN OCULAR ORGANOGENESIS PAULO MATHEUS GUERRA RIBEIRO DE SOUSA RODRIGUES, GABRIEL RODRIGUES CAVALHEIRO, PIERRE-OLIVIER FRAPPART, RODRIGO ALVES PORTELA MARTINS N - 21 MORPHOFUNCTIONAL CHARACTERIZATION OF THE PROSTATE IN CALOMYS CALLOSUS (RODENTIA, CRICETIDAE) RODENT. RENATO SIMÕES CORDEIRO, KÁRITA ROSA DE ALMEIDA, DANIELE LISBOA RIBEIRO, TATIANA CARLA TOMIOSSO, MARCELO EMÍLIO BELETTI, LUIZ ROBERTO FALLEIROS JÚNIOR, REJANE MAIRA GÓES, SEBASTIÃO ROBERTO TABOGA N-3 DEGENERATION AND CELL REGENERATION IN THE MIDGUT OF PODISUS NIGRISPINUS (HETEROPTERA: PENTATOMIDAE) DURING THE POST- 111 N - 22 HISTOLOGICAL STUDY OF THE HEMATOPOIETIC ACTIVITY OF THE MURINE PLACENTA NATHÁLIA AZEVEDO PORTILHO, PRISCILA TAVARES GUEDES, PEDRO PAULO DE ABREU MANSO, MARCELO PELAJO-MACHADO N - 23 AGE-RELATED CHANGES IN DNA AMOUNTS OF NEURONAL NUCLEI FROM MOUSE BRAIN CORTEX. ARE NEURONS POLYPLOID OR ANEUPLOID? HENRIQUE FERREIRA RODRIGUES, TAFAREL ANDRADE DE SOUZA, FLÁVIA GERELLI GHIRALDINI, MARCELO EMÍLIO BELETTI, MARIA LUIZA SILVEIRA MELLO, ALBEROT DA SILVA MORAES N - 24 KON-TIKI INITIATES FORCE-RESISTANT ATTACHMENT OF DROSOPHILA FLIGHT MUSCLES TO TENDONS. WEITKUNAT MANUELA, GRILL W. STEPHAN, SCHNORRER FRANK N - 25 ANALYSIS OF FGF-8 IN DIDELPHIS ALBIVENTRIS, A PROMISING EXPERIMENTAL MODEL FOR THE DEVELOPMENTAL BIOLOGY AREA ÍRIA GABRIELA DIAS DOS SANTOS, ALINE GONÇALVES LIO COPOLA, ERIKA CRISTINA JORGE, ADRIANA MOREIRA, JOSÉ BENTO ALVES, ALFREDO MIRANDA DE GÓES, CRISTIANE APARECIDA DE SOUSA, GERLUZA APARECIDA BORGES SILVA N - 26 ARE CALPAINS INVOLVED IN CACTUS/IKAPPAB DEGRADATION DURING MUSCLE FORMATION IN DROSOPHILA MELANOGASTER AND CHICK EMBRYONIC MUSCLES? MÁRCIO AUGUSTO BUFFOLO, HELENA M.MARCOLLA ARAUJO, CLAUDIA MERMELSTEIN N - 27 DIFFERENTIAL EFFECTS OF MASTICATORY DEPRIVATION REGIMES AND REHABILITATION ON THE LOCOMOTOR AND EXPLORATORY ACTIVITIES OF AGED ALBINO SWISS MICE. DIEGO DE JESUS SILVA, FABÍOLA DE CARVALHO CHAVES DE SIQUEIRA MENDES, ANDRÉ PINHEIRO GURGEL FELÍCIO, MARINA NEGRÃO FROTA DE ALMEIDA, MANOELA FALSONI, MARCIA LORENA FERREIRA DE ANDRADE, JOÃO BENTO TORRES NETO, CRISTOVAM WANDERLEY PICANÇO-DINIZ, MARCIA CONSENTINO KRONKA SOSTHENES N - 28 EXPRESSION OF MYC TRANSCRIPTION FACTORS IN DEVELOPING MOUSE LENS ANIELLE LINS GOMES, GABRIEL RODRIGUES CAVALHEIRO, RODRIGO ALVES PORTELA MARTINS N - 29 THE ROLE OF MYOSIN VA IN THE NEURITOGENESIS OF DORSAL ROOT GANGLIA TRKA-POSITIVE NEURONS TATIANE YUMI NAKAMURA KANNO, ENILZA MARIA ESPREAFICO, CHAO YUN IRENE YAN N - 30 CELLULAR STRESS INDUCED BY UV-RADIATION ON MACROBRACHIUM OLFERSI EMBRYOS VALQUIRIA MACHADO CARDOSO, EVELISE MARIA NAZARI, DIB AMMAR, YARA MARIA RAUH MÜLLER PEREIRA DIAS DE OLIVEIRA, JORGE JOSÉ DE CARVALHO, MARCELO PELAJO MACHADO N - 42 WNT/BETA-CATENIN SIGNALING MODULATION BY MEMBRANE MICRODOMAINS IN EMBRYONIC DEVELOPMENT ANDRESSA LUY KAJISHIMA, ALICE HELENA REIS, JOSE GARCIA ABREU N - 43 REGULATION BETWEEN WNT AND SHH SIGNALING IN FOREBRAIN DEVELOPMENT FERNANDA PEREIRA DE OLIVEIRA, ALICE HELENA DOS REIS, ANDRESSA LUY KAJISHIMA, JOSE GARCIA ABREU N - 44 MORPHOLOGICAL AND HISTOCHEMICAL STUDY OF THE LIVER IN HEMISORUBIM PLATYRHYNCHOS LARVAE AND ADULT CLAUDEMIR KUHN FACCIOLI, RENATA ALARI CHEDID, ANTONIO CARLOS DO AMARAL, IRENE BASTOS FRANCESCHINI VICENTINI, CARLOS ALBERTO VICENTINI N - 45 GLYCOSYLATION PROCESSES MODULATE BMP PATHWAY DURING DROSOPHILA MELANOGASTER DEVELOPMENT AMANDA RIBEIRO CÂMARA, ERIKA MICHELE AVELINO NEGREIROS GONÇALVES, KATIA CARNEIRO DE PAULA, HELENA MARIA MARCOLLA ARAUJO, ADRIANE REGINA TODESCHINI N - 46 HOMOCYSTEINE CAUSES DISRUPTIONS ON SPINAL CORD MORPHOLOGY AND CHANGES THE EXPRESSION OF THE PAX 1/9 AND SOX 9 GENE PRODUCTS IN THE AXIAL MESENCHYME OF THE CHICKEN KAROLINE KOBUS, GILIAN F. BOURCKHARDT, DIB AMMAR, EVELISE MARIA NAZARI, YARA MARIA RAUH MÜLLER N - 47 EARLY DEVELOPMENT OF THE NEOTROPICAL FISH, LEPORINUS OBTUSIDENS (VALENCIENES, 1836) RENATA ALARI CHEDID, CLAUDEMIR KUHN FACCIOLI, RICARDO HIDEO MORI, IRENE BASTOS FRANCESCHINI VICENTINI, CARLOS ALBERTO VICENTINI N - 48 FUNCTIONAL ANALYSIS OF RHODNIUS PROLIXUS DV PATTERNING MATEUS ANTONIO BERNI, MÁRCIO RIBEIRO FONTENELE, MARCOS HENRIQUE FERREIRA SORGINE, RODRIGO NUNES DA FONSECA, HELENA MARIA MARCOLLA ARAÚJO N - 49 STRUCTURE OF THE ESOPHAGEAL EPITHELIUM IN HEMISORUBIM PLATYRHYNCHOS LARVAE AND ADULT CLAUDEMIR KUHN FACCIOLI, RENATA ALARI CHEDID, ANTONIO CARLOS DO AMARAL, IRENE BASTOS FRANCESCHINI VICENTINI, CARLOS ALBERTO VICENTINI N - 50 THE INFLUENCE OF SONIC HEDGEHOG SIGNALLING PATHWAY IN THE EAR DEVELOPMENT LEONARDO POLON, ALICE HELENA DOS REIS, JOSE GARCIA RIBEIRO ABREU JUNIOR, JOSÉ MARQUES DE BRITO NETO N - 31 MORPHOLOGICAL AND GROWTH ANALYSIS OF SKELETAL MUSCLE IN COLOSSOMA MACROPOMUM (TAMBAQUI) DARUZI CEZAR FELIPPE, FERNANDA DE MELLO, NÍVIA LOTHHAMMER, LUIS R. J. GUERREIRO, LEANDRO C. GODOY, DANILO P. STREIT JR N - 51 MULTIPOTENT NEURAL AND SKELETOGENIC-ADIPOGENIC PROGENITORS IN THE AVIAN TRUNK NEURAL CREST JULIANA DE MATTOS COELHO AGUIAR, NICOLE LE DOUARIN, ELISABETH DUPIN N - 32 104 µM IS THE LOWEST CONCENTRATION OF 20-OH ECDYSONE THAT STILL INDUCES THE ACTIVITY OF BHSGAMP-1, A GENE THAT ENCODES AN AMP, IN THE SALIVARY GLAND OF BRADYSIA HYGIDA (DIPTERA, SCIARIDAE) GABRIELA MORILHA ZANAROTTI, JORGE CURY DE ALMEIDA O – Epigenetics N - 33 THE SMAD1 EXPRESSION IN CRANIAL NEURAL CREST CONTROL FORE- AND MIDBRAIN PATTERNING. DIEGO PINHEIRO AGUIAR, NICOLE LE DOUARIN O1-O18 N - 34 EFFECTS OF HOMOCYSTEINE ON MESENCHYMAL CELLS DURING LIMB DEVELOPMENT OF GALLUS DOMESTICUS EMBRYOS GILIAN FERNANDO BOURCKHARDT, KAROLINE KOBUS, EVELISE MARIA NAZARI, MANUELA CECCHINI, YARA MARIA RAUH MÜLLER, DIB AMMAR O -1 GENETIC AND EPIGENETIC STUDY OF THE GENE TFF1 IN GASTRIC TUMORS IN PATIENTS OF THE NORTH REGION OF BRAZIL CYNTHIA FARIAS VIEIRA DE MELO, MARISE LOPES FERMINO, RUBISTENIA MIRANDA SOARES DE ARAÚJO, TALITTA DANTAS ARRUDA, CACILDA CASARTELLI, ROMMEL RODRÍGUEZ BURBANO, ELEONIDAS MOURA LIMA N - 35 MYC PROTO-ONCOGENES REGULATE GABRIEL RODRIGUES CAVALHEIRO, ANIELLE LINS GOMES LENS DEVELOPMENT N - 36 SALDANA-CABOVERDE A, KOS L (2012). THE TRANSCRIPTION FACTORS ETS1 AND SOX10 INTERACT DURING MELANOCYTE DEVELOPMENT IN THE MOUSE EMBRYO. AMY SALDANA-CABOVERDE, LIDIA KOS N - 37 MEMBRANE METALLOPROTEINASE 1 (MT1-MMP) EXPRESSION IN THE DEVELOPMENT OF THE INTESTINAL EPITHELIUM OF RATS KAMILA CAROLINE CAMARGO, MARIA ALBERTINA DE MIRANDA SOARES, JOSÉ ROSA GOMES N - 38 ULTRAVIOLET B RADIATION (UVB) AFFECTS CELL PROLIFERATION DURING EARLY EMBRYONIC DEVELOPMENT OF FRESHWATER PRAWN MACROBRACHIUM OLFERSI. ELIANE CRISTINA ZENI, GUILHERME AUGUSTO MAIA, FRANCIELY POLLO, DIB AMMAR, YARA MARIA RAUH MÜLLER, EVELISE MARIA NAZARI N - 39 EFFECT OF METHYLMERCURY ON EMBRYONIC DEVELOPMENT OF GALLUS DOMESTICUS FABIANA DE FATIMA FERREIRA, EVELISE MARIA NAZARI, YARA MARIA RAUH MÜLLER O -2 METHYLATION PATTERN OF GENE TP53 IN GASTRIC ADENOCARCINOMA CYNTHIA FARIAS VIEIRA DE MELO, RUBISTENIA MIRANDA SOARES DE ARAÚJO, TALITTA DANTAS ARRUDA, ELEONIDAS MOURA LIMA O -3 CPG ISLAND METHYLATION OF CASPASE 8 APOPTOSIS-RELATED GENE IN HUMAN SAMPLE EPITHELIAL OVARIAN CARCINOMA LETÍCIA DA CONCEIÇÃO BRAGA, ANA PAULA ÁLVARES DA SILVA RAMOS, AGNALDO LOPES DA SILVA FILHO, LUCIANA MARIA SILVA O -4 METHYLATION PROFILE MUTLΑ SUBUNIT OF DNA MISMATCH REPAIR RUBISTENIA MIRANDA SOARES DE ARAÚJO, CYNTHIA FARIAS VIEIRA DE MELO, TALITTA DANTAS ARRUDA, ELEONIDAS MOURA LIMA O -5 DNA METHYLATION RATE AND VITAMINS CONCENTRATIONS IN WOMEN WITH RECURRENT MISCARRIAGES NATHALIA SIERRA MONTEIRO, JESSICA CARRILHO BRITTO, KELMA CORDEIRO DA SILVA GIUSTI, MÁRIO HENRIQUE BURLACCHINI DE CARVALHO, ANTÔNIO AMORIM FILHO, ELVIRA MARIA GUERRA SHINOHARA N - 40 CHOLESTEROL SYNTHESIS INHIBITION IN ZEBRAFISH MYOGENESIS LAISE MONTEIRO CAMPOS, EDUARDO ANDRÉS RÍOS MORRÍS, NAIARA RODRIGUES, CLÁUDIA DOS SANTOS MERMELSTEIN, MANOEL LUIS COSTA O -6 STUDY OF UGT1A1 GENE POLYMORPHISMS AND ASSOCIATION WITH THE ADVERSE EFFECTS IRINOTECAN: A PILOT STUDY HELEN TAIS DA ROSA, MICHELLE FRAGA EISENHARDT, MARCELO LUIS DOTTO, CÁTIA SEVERO, JULIANA FONTELLA, LIA POSSUELO N - 41 CARBOHYDRATE EXPRESSION IN AORTA-GONAD-MESONEPHROS REGION DURING EARLY CHICK EMBRYO DEVELOPMENT PATRICIA ALZUETA MORENO MARTINEZ, PRISCILA TAVARES GUEDES, BARBARA CRISTINA EUZEBIO O -7 IN SITU IDENTIFICATION AND LOCALIZATION OF NUCLEAR GLYCOPROTEINS IN CORTICAL NEURONS FROM MICE WITH THREE DIFFERENT AGES TAFAREL ANDRADE DE SOUZA, INGRID CORTIZO PRIETO, HENRIQUE 112 FERREIRA RODRIGUES, FLAVIA GERELLI GHIRALDINI, MARIA LUIZA SILVEIRA MELLO, ALBERTO DA SILVA MORAES O -8 ROLE OF SIRT1 IN THE ESTABLISHMENT OF ABERRANT EPIGENETIC MARKS ASSOCIATED WITH MELANOCYTE MALIGNANT TRANSFORMATION FABIANA MARCELINO MELISO, FERNANDA MOLOGNONI, MIRIAM GALVONAS JASIULIONIS O -9 EXPRESSION, EPIGENETIC REGULATION AND FUNCTION OF GAMMA-SYNUCLEIN IN MELANOMA PROGRESSION ANA CAROLINA MONTEIRO, CAMILA FERREIRA DE SOUZA, MIRIAM GALVONAS JASIULIONIS O -10 GENE EXPRESSION ASSESSMENT OF THE POLYCOMB & TRITHORAX COMPLEXES IN THE BRAIN OF FAT-TISSUE-IMPLANTED POLYCYSTIC OVARIAN SYNDROME MICE EDUARDO HENRIQUE DA SILVA FREITAS, SAMUEL MARCOS RIBEIRO DE NORONHA, CARLOS FERNANDES BAPTISTA, MARIA DE NAZARETH GAMBOA RITTO, JORGE KEDE, ISIDORO BINDA NETO, SILVANA APARECIDA ALVES CORREA DE NORONHA, ISMAEL DALE COTRIM GUERREIRO DA SILVA O -11 THE PATTERN OF WNT5A GENE METHYLATION DIFFERS FROM MEDULLARY THYROID CARCINOMA (MTC) CELL LINE AND WITHIN SIBLINGS CARRYING THE SAME RET C634R ACTIVATING MUTATION, AND CORRELATES WITH EARLY ONSET OF FAMILIAL MTC TUMOR MIRIAN GONÇALVES CARDOSO, MARINA MALTA LETRO KIZYS, MICHELLE YURI HARADA, DANIELA FILIPPINI IERARDI, SUSAN CHOW LINDSEY, FLÁVIA DE OLIVEIRA FACURI VALENTE, MARIA CLARA DE CARVALHO MELO, ROSANA DELCELO, JOÃO ROBERTO MACIEL MARTINS, RUI MONTEIRO DE BARROS MACIEL, MIRIAM GALVONAS JASIULIONIS, MAGNUS RÉGIOS DIAS DA SILVA O -12 EXPRESSION PROFILE AND SUBCELLULAR LOCALIZATION OF A NOVEL HUMAN LYSINE METHYLTRANSFERASE SETD4 IN BREAST CANCER JERUSA ARAÚJO QUINTÃO ARANTES FARIA, CAROLINA ANDRADE, ANA CAROLINA DE ANGELIS CAMPOS, HELENICE GOBBI, ALFREDO MIRANDA GOES, DAWIDSON ASSIS GOMES, FABIO PITTELLA SILVA O -13 EPIGENETIC MODIFIERS 5-AZA-2’-DEOXYCYTIDINE AND TRICHOSTATIN A INFLUENCE ADIPOCYTE DIFFERENTIATION OF HUMAN MESENCHYMAL STEM CELLS JAIESA ZYCH, CRISCIELE KULIGOVSKI, MARCO A STIMAMIGLIO, ALEXANDRA SENEGAGLIA, PAULO S BROFMAN, BRUNO DALLAGIOVANNA, SAMUEL GOLDENBERG, ALEJANDRO CORREA O -14 VASCULAR DYSFUNCTION IN IUGR UMBILICAL VEIN IS ACCOMPANIED WITH EPIGENETIC ALTERATIONS IN ENOS PROMOTER. BERNARDO JAVIER KRAUSE LEYTON, IVO CARRASCO WONG, PAOLA CASANELLO TOLEDO O -15 EPIGENETIC EVENTS SEEM TO BE IMPORTANT TO DYNAMIC PHENOTYPE SWITCHING ALONG MELANOCYTE MALIGNANT TRANSFORMATION ALICE SANTANA MORAIS, MIRIAM GALVONAS JASIULIONIS O -16 PROMOTER HYPERMETHYLATION OF E-CADHERIN IN ASTROCYTOMAS WALLAX AUGUSTO SILVA FERREIRA, MARIANA DINIZ ARAÚJO, SYMARA RODRIGUES-ANTUNES, MARICELI BAIA DOS SANTOS, NILSON PRAIA ANSELMO, JOSÉ REGINALDO NASCIMENTO BRITO, MARIA LÚCIA HARADA, ROMMEL MARIO RODRIGUEZ BURBANO, BÁRBARA DO NASCIMENTO BORGES O -17 METHYLATION PATTERN OF MIR-124A2 AND MIR-124A3 IN ASTROCYTIC TUMORS MARICELE BAIA DOS SANTOS, MARIANA DINIZ ARAÚJO, WALLAX AUGUSTO SILVA FERREIRA, SYMARA RODRIGUES-ANTUNES, JOSÉ REGINALDO NASCIMENTO BRITO, DOUGLAS VASCONCELOS, NILSON PRAIA ANSELMO, ROMMEL MARIO RODRIGUEZ BURBANO, MARIA LÚCIA HARADA, BÁRBARA DO NASCIMENTO BORGES O -18 REGULATION OF SUGARCANE MICRORNAS IN PATHOGENS RESPONSES BÁRBARA COSTA PEIXOTO, FLÁVIA THIBEAUT, CRISTIAN ANTONIO ROJAS, ADRIANA SILVA HEMERLY, PAULO CAVALCANTI GOMES FERREIRA P – Extracellular Matrix P1-P37 P-1 HISTOLOGICAL AND ULTRASTRUCTURAL ANALYSIS OF THE EFFECTS OF ELECTRICAL STIMULATION ON CARTILAGE HEALING IN ADULT MALE RATS (RATTUS NORVEGICUS). DENISE CRISTINA ZUZZI, CARLA DE CAMPOS CICCONE, PAULO PINTO JOAZEIRO, LAURECIR GOMES, MARCELO AUGUSTO MARRETTO ESQUISATTO P-2 STRUCTURAL FEATURES OF THE HYALINE CARTILAGE DURING REPAIR PROCESS IN IMMATURE MALE RATS (RATTUS NORVEGICUS) TREATED WITH MICROCURRENT. CARLA DE CAMPOS CICCONE, DENISE CRISTINA ZUZZI, PAULO PINTO JOAZEIRO; LAURECIR GOMES; MARCELO AUGUSTO MARRETTO ESQUISATTO P-3 PSYCHOLOGICAL STRESS AND FISH OIL SUPLEMENTATION ON CUTANEOUS WOUND HEALING ALICE DOS SANTOS ROSA, LUANA GABRIELA BANDEIRA, ANDRÉA MONTE-ALTO-COSTA, BRUNA ROMANA-SOUZA P-4 OVARIAN HORMONES EFFECT ON BIGLYCAN SECRETION BY DECIDUAL CELLS IN VITRO AMBART ESTER COVARRUBIAS CISTERNA, MARIANA CASTRO ÁVILA, VANESSA MORAIS FREITA, TELMA MARIA TENORIO ZORN P-5 HETEROLOGOUS EXPRESSION, PURIFICATION AND ACTIVITY OF A HYALURONIDASE FROM BROWN SPIDER VENOM LOXOSCELES INTERMEDIA VALÉRIA PEREIRA FERRER, THIAGO LOPES DE MARI, LUIZA HELENA GREMSKI, RAFAEL BERTONI DA SILVEIRA, OLGA MEIRI CHAIM, ANDREA SENFF RIBEIRO, SILVIO SANCHES VEIGA P-6 STRUCTURAL AND BIOCHEMICAL MODIFICATIONS IN TENDON FIBROCARTILAGE METAPLASIA OF BULLFROGS (RANA CATESBEIANA) DURING MATURATION AND AGING. VALDENILSON JOSÉ ZORÉL, LAURECIR GOMES, MARCELO AUGUSTO MARRETTO ESQUISATTO P-7 POSTMETAMORPHOSIS MATURATION OF ARTICULAR CARTILAGE FROM THREE ANATOMICAL SITES IN BULLFROG (RANA CATESBEIANA) KNEE JOINT: A STRUCTURAL STUDY. ANDRÉ HEBLING, MARCELO AUGUSTO MARRETTO ESQUISATTO, LAURECIR GOMES P-8 STRUCTURAL ANALYSIS OF THE REPAIR PROCESS IN SKIN WOUNDS EXPERIMENTALLY INDUCED IN MALE WISTAR RATS (RATTUS NORVEGICUS). THAIS LARISSA PEROTTI, MARINA VIGANÓ PENTEADO, JOSÉ EDUARDO SCABORA, MARCELO AUGUSTO MARRETTO ESQUISATTO P-9 EFFECT OF PROTEIN RESTRICTION DIET ON THE REPAIR PROCESS IN SKIN WOUNDS, EXPERIMENTALLY INDUCED, IN FEMALE WISTAR RATS (RATTUS NORVEGICUS). MARINA VIGANÓ PENTEADO, THAIS LARISSA PEROTTI, JOSE EDUARDO SCABORA, MARCELO AUGUSTO MARRETTO ESQUISATTO P - 10 EFFECTS OF MICROCURRENT AND INGAP-LASER (670NM) IN THE REPAIR OF EXPERIMENTAL WOUNDS IN HEALTHY AND ALLOXAN-DIABETIC RATS. LIA MARA GROSSO NEVES, RAFAEL LUÍS MATHEUS, MARCELO AUGUSTO MARRETO ESQUISATTO, MARIA ESMÉRIA COREZOLA DO AMARAL, GLAUCIA MARIA TECH DOS SANTOS, FERNANDA APARECIDA SAMPAIO MENDONÇA P - 11 DIFFERENTIAL DISTRIBUTION AND REMODELING OF ELASTIC SYSTEM FIBERS IN THE HEART OF SPONTANEOUSLY HYPERTENSIVE RATS MILENE SANCHES GALHARDO, MARIANA METERA VERAS, JULIANA MORA VERIDIANO, MARCELO ALVES FERREIRA, OLGA MARIA DE TOLEDO CORRÊA, MARIA CLAUDIA IRIGOYEN, ELIA GARCIA CALDINI P - 12 BETA-ADRENOCEPTOR BLOCKADE COMPROMISES THE CUTANEOUS WOUND HEALING OF CHRONIC LESIONS THATIANA L. ASSIS DE BRITO, ANDRÉA MONTE-ALTO-COSTA, BRUNA ROMANA-SOUZA P - 13 ULTRASTRUCTURAL AND BIOCHEMICAL ANALYSIS OF EXTRACELLULAR MATRIX IN SCIATIC NERVE FROM WISTAR MALE RATS DURING MATURATION AND AGING HALINE BALLESTERO FÊO, ANDREA APARECIDA DE ARO, LAURECIR GOMES, MARCELO AUGUSTO MARRETTO ESQUISATTO P - 14 BIOCHEMISTRY AND HISTOCHEMISTRY OF OLIGOCHAETAS: LOCALIZATION AND CHARACTERIZATION OF SULFATED GLYCOSAMINOGLYCANS IN THE BODY OF THE EARTHWORM EISENIA ANDREI LAINA CRISTINA FERREIRA, RITA DE CASSIA LIMA MARTINS, CELIA YELIMAR PALMERO QUINTANA, LUIZ EURICO NASCIUTTI, LUIZ CLAUDIO FRANCISCO DA SILVA P - 15 EFFECT OF THE ALOE VERA OINTMENT IN THE REORGANIZATION OF THE COLLAGEN FIBERS DURING TENDON HEALING ANDREA APARECIDA DE ARO, BENEDICTO DE CAMPOS VIDAL, UMAR NISHAN, MYLENA OLIVEIRA PEREZ, RODNEY A. RODRIGUES, MARY ANN FOGLIO, JOAO ERNESTO DE CARVALHO, LAURECIR GOMES, EDSON ROSA PIMENTEL P - 16 EFFECT OF THE CALENDULA OFFICINALIS CREAM DURING THE INFLAMMATORY PHASE OF TENDON HEALING MYLENA OLIVEIRA PEREZ, ANDREA APARECIDA DE ARO, CRISTIANO PEDROZO VIEIRA, RODNEY A. RODRIGUES, LAURECIR GOMES, EDSON ROSA PIMENTEL P - 17 BIOMECHANICAL PROPERTIES OF INTERPUBIC TISSUES DURING PREGNANCY AND AFTER DELIVERY OF MICE C57BL6 M. M. M. SILVA, V. S. ROSA, S. R. CONSONNI, L. C. V. ALVES, P. P. JOAZEIRO P - 18 THE FOLLICULAR THYROID CELL LINE PCCL3 DIFFERENTIALLY RESPONDS TO LAMININ AND TO POLYLAMININ, A POLYMERIC FORM OF LAMININ ASSEMBLED AT ACIDIC PH CELIA YELIMAR PALMERO QUINTANA, LEANDRO MIRANDA ALVES, MADALENA M. SANT’ANA BARROSO, ELAINE C. DE SOUZA, DANIEL E. MACHADO, ANTONIO PALUMBO JUNIOR, CARLOS A. DO NASCIMENTO, CLAUDIA S. MERLMESTEIN, CHRISTINA M. TAKIYA, DENISE P. CARVALHO, CAMILA HOCHMAN MENDEZ, TATIANA COELHO-SAMPAIO, LUIZ EURICO NASCIUTTI P - 19 COLLAGEN GLYCATION TO GENERATE SKIN RECONSTRUCTED MIMICKING AGED AND DIABETIC SKIN IN VITRO PAULA COMUNE PENNACCHI, MAÍRA ESTANISLAU SOARES DE ALMEIDA, MARIA CLARA DE ARAÚJO CREPALDI, MARINILCE FAGUNDES DOS SANTOS, SILVYA STUCHI MARIA-ENGLER P - 20 EXTRACELLULAR MATRIX ALTERATIONS IN INTERVERTEBRAL DISC DEGENERATION MARIA BETHANIA ROSSI PIVA, LILIAN ZERBINATTI OLIVEIRA, CINTIA PEREIRA OLIVEIRA, CAMILA DE MELO ACCARDO, IVARNE LUIS DOS SANTOS TERSARIOL, HELENA BONCIANI NADER, LUCIANO MILLER REIS RODRIGUES, MARIA APARECIDA DA SILVA PINHAL 113 P - 21 IN VITRO EFFECTS OF SILVER NANOPARTICLES ON HUMAN FIBROBLAST LARISSA FERNANDA DE ARAUJO VIEIRA, IANA MAYANE MENDES NICÁCIO VIANA, CÁSSIO ERÁCLITO ALVES DOS SANTOS, ANA RÚBIA BATISTA RIBEIRO, JANDIR MIGUEL HICKMANN, SALETE SMANIOTTO Q – Gene Therapy P - 22 EVALUATION OF FIBRILLIN-1'S ROLE IN ARTERIAL THROMBOGENESIS. PLATELETS PROTEOMIC ANALYSIS. CATHERINE NATALIA PEREIRA, DANIEL MARTINS-DE-SOUZA, ADRIANA PAES LEME, LYGIA V. PEREIRA, CRISTINA P. VICENTE, JOSÉ CAMILLO NOVELLO, CLAUDIO C. WERNECK Q1-Q5 P - 23 NOVEL ALPL GENETIC ALTERATION AFFECTING THE TNAP COLLAGEN BINDING DOMAIN IS ASSOCIATED WITH AN ODONTOHYPOPHOSPHATASIA PHENOTYPE LUCIANE MARTINS, THAISÂNGELA L. RODRIGUES, MARIANA MARTINS RIBEIRO, MIKI TAKETOMI SAITO, ANA PAULA OLIVEIRA GIORGETTI, ENILSON ANTONIO SALLUM, MÁRCIO ZAFFALON CASATI, FRANCISCO HUMBERTO NOCITI JUNIOR P - 24 IN VITRO EFFECTS OF INSULIN-LIKE GROWTH FACTOR-1 AND CHEMOKINE LIGAND-2 ON ENDOTHELIAL CELLS IANA MAYANE MENDES NICÁCIO VIANA, MAÍRA ESTANISLAU SOARES DE ALMEIDA, MARIA DANIELMA DOS SANTOS REIS, MARVIN PAULO LINS, SILVANA AYRES-MARTINS, SALETE SMANIOTTO P - 25 METALOPROTEINASES, MYOFIBROBLASTS AND KI-67 EVALUATION IN KERATOCYSTIC ODONTOGENIC TUMOR AND FOLLICLE PERICORONAL GRASIELI DE OLIVEIRA RAMOS, ALINE COSTA, JULIANA CRISTINA PORTO, DANIELLA SERAFIN COUTO VIEIRA, ELENA RIET CORREA RIVERO P - 26 INHIBITION OF TGF-Β PATHWAY REVERTS EXTRACELLULAR MATRIX REMODELING IN T. CRUZI-INFECTED CARDIAC MICROTISSUES PATRÍCIA MELLO FERRÃO, MARIANA CALDAS WAGHABI, LUCIANA RIBEIRO GARZONI P - 27 DISTRIBUTION OF PROTEOGLYCANS IN ANEURYSM AND DISSECTION OF THE HUMAN ASCENDING AORTA THIAGO HENRIQUE SCHULTZ, VINÍCIUS ORNELAS BICALHO, RICARDO RIBEIRO DIAS, NOEDIR ANTONIO GROPPO STOLF, PAULO SAMPAIO GUTIERREZ, LUCIANO DE FIGUEIREDO BORGES P - 28 MESTEROLONE AND AEROBIC EXERCISE EFFECTS ON THE ECM OF THE TAIL TENDON OF C57BL/6 TRANSGENIC MICE. TATIANA CARLA TOMIOSSO, KARINA FONTANA, MARIA ALICE DA CRUZ HÖFLING G, EDSON ROSA PIMENTEL P - 29 EXPRESSION AND DISTRIBUTION OF ANNEXIN II IN ANEURYSM AND DISSECTION OF THE HUMAN ASCENDING AORTA SÁVIO MELO RABELO, MILLANE VIEIRA SANTOS, RICARDO RIBEIRO DIAS, NOEDIR ANTONIO GROPPO STOLF, PAULO SAMPAIO GUTIERREZ, LUCIANO DE FIGUEIREDO BORGES P - 30 DERMAL EQUIVALENT WITH MELANOMA AS A PLATFORM TO SCREENING ANTITUMORAL MOLECULES MANOELA TIAGO DOS SANTOS, CARLA ABDO BROHEM, EDSON MENDES OLIVEIRA, RAFAEL DUARTE PAES, ANA CAMPA, SILVIA BERLANGA DE MORAES BARROS, SILVYA STUCHI MARIA ENGLER P - 31 ROLE OF LAMININ ISOFORMS IN THE PROLIFERATION, MIGRATION, DIFFERENTIATION AND DEATH OF HUMAN MYOBLASTS: APPLICATION FOR CELL THERAPY INGO RIEDERER, ELISA NEGRONI, DANIELLE INGRID BEZERRA DE VASCONCELOS, ELIANE CORREA DE SANTANA, DAIANE CRISTINA FERREIRA GOLBERT, MARCELO RIBEIRO ALVES, CAMILA SANCHES, SORAYA CHAOUCH, GILLIAN SANDRA BUTLER BROWNE, VINCENT MOULY, WILSON SAVINO P - 32 INFLUENCE OF OPN ON MINERALIZATION OF OSTEOGENIC CELLS CULTURED ON A NANOSTRUCTURED TITANIUM SURFACE ADRIEL HENRIQUE PEIXOTO DA SILVA GERALDO, FABÍOLA SINGARETTI DE OLIVEIRA, RICARDO DELLA COLETTA, ADALBERTO LUIZ ROSA, PAULO TAMBASCO DE OLIVEIRA, PATRICIA ADACHI P - 33 TRITERPENE UVAOL STIMULATES PHAGOCYTOSIS AND EXTRACELLULAR MATRIX PRODUCTION IN PERITONEAL MACROPHAGES IN VITRO. REBEKA RAÍSA SOUZA DE MELO, IANA MAYANE MENDES NICÁCIO VIANA, LARISSA FERNANDA DE ARAÚJO VIEIRA, ALTAIR ROGÉRIO ALVES BRANDÃO, EMILIANO BARRETO, SALETE SMANIOTTO P - 34 TISSUE ENGINEERED THREE DIMENSIONAL IN VITRO: DECELLULARIZATION AND RECELULARIZATION OF THE KIDNEYS. ANDREZA BASTOS MARTINS, BERNARDO JORGE DA SILVA MENDES, ANTONIO CARLOS CAMPOS DE CARVALHO, JORGE LUIZ DA CUNHA MORAES, JOSE ROBERTO SILVA, RODRIGO NUNES DA FONSECA, CINTIA MONTEIRO DE BARROS, JACKSON DE SOUZA MENEZES, REGINA COELI DOS SANTOS GOLDENBERG P - 35 HEPARANASE-1 SILENCING COMPROMISES SMOOTH MUSCLE CELL DIFFERENTIATION DURING VENTRAL PROSTATE MORPHOGENESIS IN VITRO GUILHERME OLIVEIRA BARBOSA, TAIZE AUGUSTO MACHADO, ALEXANDRE BRUNI CARDOSO, HERNANDES F. CARVALHO P - 36 EFFECT OF THE TREATMENT WITH STATINS ON THE DEEP DIGITAL FLEXOR TENDON OF RATS LETÍCIA PRADO DE OLIVEIRA, CRISTIANO PEDROZO VIEIRA, FLÁVIA DA RÉ GUERRA, EDSON ROSA PIMENTEL P - 37 CHRONIC ADMINISTRATION OF STATINS CAUSES BIOCHEMICAL CHANGES IN ACHILLES TENDON LETÍCIA PRADO DE OLIVEIRA, CRISTIANO PEDROZO VIEIRA, FLÁVIA DA RÉ GUERRA, EDSON ROSA PIMENTEL Q -1 GM-CSF GENE THERAPY TO RABBIT HIND LIMB ISCHEMIA LEONARDO MARTINS SILVA, VIVIAN YOSHIKO SAMOTO, PRISCILA MARTINS ANDRADE DENAPOLI, LEONARDO PINTO CARVALHO, ALEXANDRE SOUTO, JOÃO CARLOS COSTA BAPTISTA SILVA, SANG WON HAN Q -2 MESENCHYMAL STEM CELLS LOWER PROLIFERATION AND INVASION OF GLIOBLASTOMA CELLS, EXPLOITING THE IMMUNE RESPONSE MEDIATING CHEMOKINES TAMARA T. LAH, MOTALN H, GRUDEN K, HREN M, PRIMON M, SCHICHOR CH Q -3 INHIBITORY EFFECT OF ENDOSTATIN ON TUMOR CELL DENSITY IN METASTATIC RENAL CELL CARCINOMA ZENÓBIO ANTONIO VIANA DE BARROS, MARINA DE SOUZA BRAGA, KAREN CRISTINA BARBOSA CHAVES, MARIA HELENA BELLINI Q -4 INTRAPERITONEAL INJECTION EFFECT OF MESENCHYMAL STEM CELLS MODIFIED WITH IDUA TO TREAT MUCOPOLYSACCHARIDOSIS TYPE I IN MPSI MOUSE PRISCILA KEIKO MATSUMOTO MARTIN, ROBERTA SESSA STILHANO, FLAVIA HELENA DA SILVA, VIVIAN YOSHIKO SAMOTO, GIOVANI BRAVIN PERES, SANG WON HAN Q -5 EXPRESSION OF A PROAPOPTOTIC MYOSIN VA FRAGMENT RETARDS MELANOMA TUMOR GROWTH IN ANIMAL MODEL ANTONIO CARLOS BORGES, PABLO MARCO PEIXOTO, ANA PAULA BARRETO DE PAIVA, DENISE PIMENTA DA SILVA LEITAO-MAZZI, KATHLEEN W KINNALLY, ENILZA MARIA ESPREAFICO R – Host-Parasite Interaction R1-R75 R -1 EVALUATION OF JOANNESIA PRINCEPS AQUEOUS EXTRACT ANTHELMINTIC ACTIVITY IN NATURALLY INFECTED MICE BY SYPHACIA OBVELATA, ASPICULURIS TETRAPTERA AND VAMPIROLEPIS NANA. HELCIO RESENDE BORBA, JENNIFER VIEIRA GOMES, JESSICA TAMARA DOS SANTOS TEIXIERA, MARIANA DA SILVA DE MELLO, PATRÍCIA FAMPA, LENÍCIO GONÇALVES, FRANCISCO DE ASSIS DA SILVA, VIVIANE MOREIRA DE LIMA R -2 THE DIVERSE AND DYNAMIC NATURE OF LEISHMANIA PARASITOPHOROUS VACUOLES STUDIED BY MULTIDIMENSIONAL IMAGING FERNANDO ROBERTO OLIVEIRA REAL, RENATO ARRUDA MORTARA R -3 MORPHOLOGICAL CHANGES IN THE CYST WALL OF THE PARASITE GIARDIA LAMBLIA DURING EXCYSTATION PROCESS VICTOR DO VALLE PEREIRA MIDLEJ, ISADORA PEIXOTO MEINIG, MARLENE BENCHIMOL R -4 TRITRICHOMONAS FOETUS EXPRESSES DIFFERENT ECTOPHOSPHATASE ACTIVITIES DURING THE PSEUDOCYST FORMATION ANTONIO PEREIRA-NEVES, JOSÉ ROBERTO MEYER-FERNADES, MARLENE BENCHIMOL R -5 PRIMARY CULTURES OF SEVERAL TISSUES FROM THE MOLLUSK BIOMPHALARIA TENAGOPHILA (ORBIGNY, 1835), VECTOR OF SCHISTOSOMIASIS ARISTEU SILVA NETO, AMANDA CIPRIANO ARAÚJO, MARIANA PEDROSA GARCIA, CONSUELO LATORRE FORTES DIAS, PAULO MARCOS ZECH COELHO, LUCIANA MARIA SILVA R -6 STRUCTURAL AND ULTRASTRUCTURAL CHARACTERIZATION OF PRIMARY CULTURE CELLS FROM DIFFERENT TISSUES OF BIOMPHALARIA TENAGOPHILA ARISTEU SILVA NETO, LUIZ CARLOS ALVES, FÁBIO ANDRÉ BRAYNER DOS SANTOS, CONSUELO LATORRE FORTES DIAS, PAULO MARCOS ZECH COELHO, LUCIANA MARIA SILVA R -7 INVESTIGATION ON THE BEHAVIOR OF TRICHOMONAS TENAX ÉRIKA BERTOZZI, IVONE DE ANDRADE ROSA, LUIZ CARLOS DOS SANTOS RIBEIRO, MARLENE BENCHIMOL R -8 IDENTIFICATION OF FLAGELLATED PROTISTS IN THE GUT OF THE TERMITE COPTOTERMES GESTROI LIGIA FERREIRA NASCIMENTO, SEVERINO A. LUCENA, REGINALDO CONSTANTINO, MARLENE BENCHIMOL R -9 LYSOSOME DYNAMICS: WHAT IS THE IMPORTANCE OF CHOLESTEROL FOR T. CRUZI ENTRY INTO CELLS? BÁRBARA HISSA DE CARVALHO VIEIRA COUTO, PAULA MAGDA DA SILVA ROMA, ANA PAULA ALVES, FÁBIO PEREIRA SANTOS, ANA MARIA DE PAULA, UBIRAJARA AGERO BATISTA, OSCAR NASSIF DE MESQUITA, CRISTINA GUATIMOSIM FONSECA, LUCIANA DE OLIVEIRA ANDRADE 114 R -10 COMPARATIVE STUDY OF SYNTHESIZED PENTAVALENT ANTIMONIAL COMPOUNDS WITH GLUCANTIME IN MURINE MODEL KELLY CRISTINA KATO, ADRIEL ARAÚJO FERNANDES FERREIRA, ELIANE DE MORAIS TEIXEIRA, ANA LÚCIA TELES RABELLO, CYNTHIA PERES DEMICHELI, MARCELA SANTOS PROCÓPIO, FREDERIC JEAN GEORGES FREZARD, JOSÉ DIAS CORRÊA JUNIOR R -11 SALIVARY GLAND AS A SOURCE OF NEUROTROPHIC FACTOR IN EXPERIMENTAL TRYPANOSOMIASIS LUCIANA DE OLIVEIRA ANDRADE, RICARDO TOSHIO FUGIWARA, LUISA ALMEIDA FIGUEIREDO, EGLER CHIARI, PATRICIA MASSARA MARTINELLI, PATRICIA MASSARA MARTINELLI R -12 CYTOKINE POLYMORPHISMS ANALYSIS IN PATIENT WITH AMERICAN CUTANEOUS LEISHMANIASIS FÁBIO RIBEIRO QUEIROZ, FLÁVIA PERRIM DE MELO, LETICIA DA CONCEIÇÃO BRAGA, ANA CRISTINA DE CARVALHO BOTELHO, LINTON WALLIS FIGUEIREDO SOUZA, LUCIANA MARIA SILVA R -13 IMMUNE RESPONSE IN RAT NEURON-GLIA CO-CULTURES INFECTED WITH NEOSPORA CANINUM: ROLE OF OXIDE NITRIC SYNTHETASE INDUCIBLE ALEX BARBOSA DOS SANTOS, ERICA ETELVINA VIANA DE JESUS, GREGORY FERRAZ, ALEXANDRE MORAES PINHEIRO, CÁTIA SUSE RIBEIRO, MAIARA REIS ARRUDA, ISABELA COSTA BARRETO DE ALMEIDA, SILVIA COSTA, MARIA DE FÁTIMA DIAS COSTA R -14 DECREASE OF CCL2 IN MOUSE INFECTED WITH LEISHMANIA BRAZILIENSIS IN THE PRESENCE OF ADENOSINE SAMARA FREIRE VALENTE, THAIS VIANA FIALHO, LEANDRO LICURSI DE OLIVEIRA, SÉRGIO OLIVEIRA DE PAULA, LUÍS CARLOS CROCCO AFONSO, EDUARDO DE ALMEIDA MARQUES DA SILVA R -15 REORGANIZATION OF THE MYOCARDIAL MORPHOLOGY AND CARDIOMYOCYTES MECHANICAL PROPERTIES IN EXPERIMENTAL TRYPANOSOMA CRUZI INFECTION RÔMULO DIAS NOVAES, ARLETE RITA PENITENTE, MARTA ROCHA ARAÚJO, REGGIANI VILELA GONÇALVES, ANDRÉ TALVANI, CLÓVIS ANDRADE NEVES, ANTÔNIO JOSÉ NATALI, IZABEL REGINA DOS SANTOS COSTA MALDONADO R -16 EFFECT OF PREINFECTION TREADMILL TRAINING ON THE MORPHOLOGY AND FUNCTION OF CARDIOMYOCYTES IN A MURINE MODEL OF CHAGAS’ CARDIOMYOPATHY RÔMULO DIAS NOVAES, ARLETE RITA PENITENTE, MARTA ROCHA ARAÚJO, REGGIANI VILELA GONÇALVES, ANDRÉ TALVANI, CLÓVIS ANDRADE NEVES, ANTÔNIO JOSÉ NATALI, IZABEL REGINA DOS SANTOS COSTA MALDONADO R -17 APOE4/4 TARGETED REPLACEMENT AND APOE KNOCKOUT WEANLING MICE HAVE DISTINCT ADAPTATIONS FOLLOWING CRYPTOSPORIDIUM PARVUM INFECTION AND MALNUTRITION ORLEÂNCIO GOMES RIPARDO DE AZEVEDO, DAVID BOLICK, ALDO A. M. LIMA, MICHEL P. VITEK, REINALDO B. ORIA, JAMES K. ROCHE, RICHARD L. GUERRANT R -18 ISOLATION AND PURIFICATION OF LEISHMANIA INFANTUM/CHAGASI LECTINS. THAÍS VIANA FIALHO MARTINS, SAMARA FREIRE VALENTE, SÍLVIA ALMEIDA CARDOSO, SÉRGIO OLIVEIRA DE PAULA, LEANDRO LICURSI DE OLIVEIRA, EDUARDO DE ALMEIDA MARQUES DA SILVA R -19 HEMOLYMPH CELLS OF LYMNAEA COLUMELLA, SAY, 1817, A VECTOR FOR FASCIOLOSIS IN BRAZIL VINICIUS MARQUES ANTUNES RIBEIRO, ARISTEU SILVA NETO, LUCIANA MARIA SILVA, WALTER DOS SANTOS LIMA R -20 COMPARISON OF THE ADHESION TAX OF WILD AND MUTANTS STRAINS HAEMOPHILUS INFLUENZAE IN HEC1B AND A549 CELL LINES JULIA NOGUEIRA VARELA, MÁRIO SÉRVULO IZIDORO, JR., DANILO ANTONINI ALVES, GISELE CRISTIANE GENTILLE CURY, LUCIANA MARIA DE HOLLANDA, RAFAELLA FABIANA CARNEIRO PEREIRA, MARCELO LANCELLOTTI R -21 WHY COINFECT CELLS WITH NON-VIRAL PATHOGENS? MICHEL RABINOVITCH R -22 IMMUNOHISTOCHEMICAL DETECTION OF GP43 IN THE LIVER OF SWISS MICE WITH PARACOCCIDIOIDOMYCOSIS ELAINE SCIUNITI BENITES MANSANO, TEREZINHA INEZ ESTIVALET SVIDZINSKI, EDILAINE MARTINS MORATTO, MARIANA CRISTINA VICENTE UMADA ZAPATER, MARIA DOS ANJOS FORTUNATO, LUZMARINA HERNANDES R -23 HISTOPATHOLOGICAL EVALUATION AND PHYSICOCHEMICAL BY RAMAN SPECTROSCOPY IN LIVER OF SWISS MICE WITH PARACOCCIDIOIDOMYCOSIS ELAINE SCIUNITI BENITES MANSANO, TEREZINHA INEZ ESTIVALET SVIDZINSKI, EDILAINE MARTINS MORATTO, MAURO LUCIANO BAESSO, GUTIERREZ RODRIGUÊS DE MORAIS, LUZMARINA HERNANDES R -24 THE TREATMENT TACHYZOITES OF TOXOPLASMA GONDII ON THE HOST CELL INTERACTION INDUCED CONVERSION FOR BRADYZOITES FORMS LOYZE PAOLA OLIVEIRA DE LIMA, THAYANE RITA BORGES DE FARIA, JULIANA DE ARAÚJO PORTES, PEDRO SOUTO RODRIGUES, RENATO AUGUSTO DAMATTA, WANDERLEY DE SOUZA, SERGIO HENRIQUE SEABRA R -25 GPI PBPGA1P OF P. BRASILIENSIS IS A SURFACE ANTIGEN THAT ACTIVATES MAST CELLS THROUGH THE TRANSCRIPTION FACTOR NF-KAPPA B INDEPENDENT OF THE HIGH AFFINITY IGE RECEPTOR CLARISSA XAVIER RESENDE VALIM, LUISA KARLA ARRUDA, CONSTANCE OLIVER, PAULO SERGIO RODRIGUES COELHO, MARIA CELIA JAMUR R -26 MAY THE ADIPOSE TISSUE BE A TRYPANOSOMA CRUZI RESERVOIR IN PATIENTS WITH CHRONIC CHAGAS DISEASE? ADALIENE VERSIANI MATOS FERREIRA, MARCELA SEGATTO DO CARMO, ZÉLIA MENEZES, ÍTALO FARIA DO VALLE, ANDRÉA MARA MACEDO, CLÁUDIO GELAPE, LUCIANA DE OLIVEIRA ANDRADE, FNU NAGAJYOTHI, PHILIPP E. SCHERER, MAURO MARTINS TEIXEIRA, HERBERT B. TANOWITZ R -27 NEUTROPHIL EXTRACELLULAR TRAPS (NETS) DECREASE THE VIABILITY OF TOXOPLASMA GONDII GABRIELA VERAS DE MORAES, TATIANA PAREDES SANTOS, ANDERSON GUIMARÃES COSTA, ELVIRA SARAIVA, MARCIA ATTIAS R -28 BINDING OF THE WHEAT GERM LECTIN TO CRYPTOCOCCUS NEOFORMANS CHITOOLIGOMERS IMPAIRS EXTRACELLULAR POLYSACCHARIDE RELEASE, TLR2-MEDIATED INTERACTION WITH PHAGOCYTES, AND BRAIN COLONIZATION IN MICE. FERNANDA L. FONSECA, ALLAN J. GUIMARÃES, FABIANNO F. DUTRA, FERNANDA D. SILVA, JULIAN E. MUÑOZ, CARLOS P. TABORDA, MARCELO T. BOZZA, LEONARDO NIMRICHTER, ARTURO CASADEVALL, MARCIO L. RODRIGUES R -29 ULTRASTRUCTURAL ANALYSIS OF HOST CELL CYTOSKELETON DURING TOXOPLASMA GONDII INVASION THAYANA ARAUJO DA CRUZ, TATIANA C. PAREDES SANTOS, WANDERLEY DE SOUZA, MARCIA ATTIAS R -30 CRYPTOCOCCUS NEOFORMANS-MACROPHAGE INTERACTION: CYTOSKELETON INVOLVEMENT UPON HOST CELL ENTRY CAROLINE REZENDE GUERRA, SERGIO HENRIQUE SEABRA, SONIA ROZENTAL R -31 IFN-GAMMA PLAYS A UNIQUE ROLE IN PROTECTION AGAINST LOW VIRULENT TRYPANOSOMA CRUZI STRAIN ADELE AUD RODRIGUES, FABIANA SILVA, FLÁVIA ALVES MARTINS, ANA FLÁVIA OLIVEIRA NOTÁRIO, ALINE ALVES DA SILVA, CECÍLIO PURCINO DA SILVA SOUZA NETO, JASSON SEBASTIAN SANABRIA SAOSA, GRACE KELLY DA SILVA, CATARINA V. HORTA, DARIO S. ZAMBONI, JOÃO SANTANA DA SILVA, ELOISA A. V. FERRO, CLAUDIO VIEIRA DA SILVA R -32 THE ROLE OF CYTOSKELETON, COMPONENTS OF IP3/DAG SIGNALING PATHWAY AND IRON IN EHRLICHIA CANIS PROLIFERATION KARINE CANUTO LOUREIRO DE ARAUJO, MARCELO ARANTES LEVENHAGEN, ROSIANE NASCIMENTO ALVES, SUSANA ELISA RIECK, MARCELO BAHIA LABRUNA, MARCELO EMÍLIO BELETTI R -33 PURIFICATION PROTOCOLS OF A 21 KDA RECOMBINANT PROTEIN BASED ON THE NATIVE TRYPANOSOMA CRUZI P21: A TOOL TO STUDY ITS BIOLOGICAL FUNCTIONS DURING PARASITE-HOST INTERACTION MARLUS ALVES DOS SANTOS, CLAUDIO VIEIRA DA SILVA, PAULA CRISTINA BRIGIDO, KARINE CANUTO LOUREIRO DE ARAÚJO, PRISCILA CASTRO CORDEIRO FERNANDES R -34 ATYPICAL ENTEROPATHOGENIC ESCHERICHIA COLI (AEPEC) SECRETES A SOLUBLE ANTI-PHAGOCYTIC FACTOR KEYDE CRISTINA MARTINS DE MELO, RAFAEL MARQUES PORTO, DANIEL CARVALHO PIMENTA, RITA DE CASSIA RUIZ R -35 3D VIEW OF INTERCONNECTIONS BETWEEN ENDOPLASMIC RETICULUM PROFILES AND INNER MEMBRANE COMPLEX OF TOXOPLASMA GONDII TATIANA CHRISTINA PAREDES SANTOS, WANDERLEY DE SOUZA, MARCIA ATTIAS R -36 TRYPANOSOMA CRUZI EXTRACELLULAR AMASTIGOTES (EAS) AND HOST CELL SIGNALING: MORE PIECES TO THE PUZZLE DIANA BAHIA, ALEXIS BONFIM-MELO, ÉDEN RAMALHO FERREIRA, RENATO ARRUDA MORTARA R -37 ROLE OF REACTIVE OXYGEN SPECIES (ROS) ON NEUTROPHIL EXTRACELLULAR TRAPS (NETS) INDUCED BY LEISHMANIA AMAZONENESIS NATALIA CADAXO ROCHAEL, MICHELLE TANNY CUNHA DO NASCIMENTO, ANDERSON BAPTISTA GUIMARÃES-COSTA, MATHEUS PINTO DE OLIVEIRA, MARCUS FERNANDES DE OLIVEIRA, ELVIRA MARIA SARAIVA R -38 INVESTIGATION OF YELLOW FEVER VIRUS-INDUCED ENDOPLASMIC RETICULUM STRESS DANIEL SANCHES, CLAUDIA MONTEIRO DA ROCHA, SAMIR PEREIRA DACOSTA CAMPOS, LUCIANE PINTO GASPAR, MARCOS DA SILVA FREIRE, BRUNO SOUZA GONÇALVES, LUCIANA BARRETO CHIARINI, JERSON LIMA DA SILVA, ANDRE MARCO DE OLIVEIRA GOMES, ANDRÉA CHEBLE DE OLIVEIRA R -39 MEDICINAL PLANT EXTRACTS: AN ALTERNATIVE APPROACH TO CONTROL INTRACELLULAR MULTIPLICATION OF LEISHMANIA AMAZONENSIS SAMUEL COTA TEIXEIRA, THAISE LARA TEIXEIRA, MARIA APARECIDA DE SOUZA, CLAUDIO VIEIRA DA SILVA R -40 NADPH OXIDASE ACTIVATION IS ASSOCIATED WITH INVASION OF HUMAN UMBILICAL VEIN ENDOTHELIAL CELLS BY GROUP B STREPTOCOCCUS JESSICA SILVA SANTOS DE OLIVEIRA, RAFAELA SILVA DOS SANTOS, PRESCILLA EMY NAGAO, GABRIELA DOS SANTOS JONATHAN R -41 PROTECTIVE MECANISMS IN THE SMALL INTESTINE INDUCED BY PREVIOUS TREATMENT WITH THE SOLUBLE ANTIGEN OF TACHYZOITES (STAG) IN MICE INFECTED ORALLY WITH TOXOPLASMA GONDII ALEXSANDRA ALVES BEZERRA MARTINS, LUCIANA ALVES DE SOUSA, LETÍCIA BRUNO, TÚLIO HENRIQUE DE FREITAS, PAULO VICTOR CZARNEWSKI BARENCO, ESTER CRISTINA BORGES ARAÚJO, NEIDE MARIA SILVA R -42 EFFECTS OF SALMONELLA ENTERITIDIS SEROVAR TYPHIMURIUM INFECTION IN ADENOCARCINOMIC HUMAN ALVEOLAR BASAL EPITHELIAL CELLS A549: PATHOGEN INDUCES APOPTOSIS JULIA NOGUEIRA VARELA, MÁRIO SÉRVULO IZIDORO JR., DANILO ANTONINI ALVES, RAFAELLA FABIANA CARNEIRO 115 PEREIRA, LUCIANA MARIA DE HOLLANDA, MARCELO BROCCHI, MARCELO LANCELLOTTI ROQUE, SILVIA L. LAGE, ROSSANA MELO, HUGO C. CASTRO-FARIA-NETO, HELOISA D'AVILA, PATRICIA TORRES BOZZA R -43 IN VITRO INTERACTION OF AEROMONAS SPP. STRAINS WITH HEP2 AND CACO-2 CELL LINES ANDRÉA FONSECA FERREIRA, PAULA AZEVEDO DOS SANTOS, ANGELA CORRÊA DE FREITAS ALMEIDA R -62 MACROPHAGE MIGRATION INHIBITORY FACTOR ACTS AS A KEY REGULATOR OF LIPID METABOLISM DURING DENGUE VIRUS INFECTION GISELLE BARBOSA DE LIMA, LÍGIA DE ALMEIDA PAIVA, IRANAIA ASSUNÇÃO MIRANDA, MARCELO TORRES BOZZA, ANDREA THOMPSON DA POIAN, PATRÍCIA TORRES BOZZA R -44 SCREENING OF NEW COMPOUNDS THAT INHIBIT THE REPLICATION OF HIV-1 SANDRO COSTA POEYS, LÍDIA MOREIRA LIMA, LUCIANA JESUS DA COSTA R -45 CYTOTOXIC ACTIVITY OF AEROMONAS STRAINS ISOLATED FROM CLINICAL SAMPLES FOR A HUMAN INTESTINAL CELL LINE (HRT-18) ANA CAROLINE WIPPICH, STELA COSTA, SUELEN WOLF, CYNTIA MARIA TELLES FADEL-PICHET, KATIA SABRINA PALUDO R -46 IN VITRO ANALYSIS OF NEISSERIA MENINGITIDIS’ INFECTION IN HUMAN IMMORTALIZED CELLS RAFAELLA FABIANA CARNEIRO PEREIRA, MÁRIO SÉRVULO IZIDORO JÚNIOR, RENAN KOSSEKI JACINTO, MARCELO LANCELLOTTI R -47 THE ROLE OF THE LAS GENE IN THE VIRULENCE OF HAEMOPHILUS INFLUENZAE BIOGROUP AEGYPTIUS ASSOCIATED WITH BRAZILIAN PURPURIC FEVER GISELE CRISTIANE GENTILE CURY, RAFAELLA FABIANA CARNEIRO PEREIRA, LUCIANA MARIA DE HOLANDA, MARCELO LANCELLOTTI R -48 TRANSLATIONAL REGULATION OF IMMUNODEFICIENCY TYPE 1 VIRUS (HIV-1) BY POLIOVIRUS 2A PROTEASE RAQUEL AMORIM, SARA MESQUITA COSTA, EDSON ELIAS DA SILVA, LUCIANA JESUS DA COSTA R -49 TOXOPLASMA GONDII PERSISTENCE IN ACTIVATED MACROPHAGES: INOS DEGRADATION MECHANISMS JULIANA DA CRUZ PADRÃO, GABRIEL RABELLO DE ABREU CABRAL, SERGIO HENRIQUE SEABRA, MARIA DE FÁTIMA SARRO DA SILVA, RENATO AUGUSTO DAMATTA R -50 ABSENCE OF LENTIVIRAL ACCESSORY PROTEIN NEF INCREASES HIV-1 PR CATALYTIC ACTIVITY REDUCING MATURE VIRAL PARTICLES PRODUCTION AND ENZYME INCORPORATION LUIZA MONTENEGRO MENDONÇA, SANDRO COSTA POEYS, LUCIANA JESUS DA COSTA R -51 GALECTIN-3 PARTICIPATION IN TRYPANOSOMA CRUZI CELL INVASION, INTRACELLULAR TRAFFIC AND MULTIPLICATION ALINE ALVES DA SILVA, FABRÍCIO CASTRO MACHADO, REBECCA TAVARES E SILVA, CLAUDIO VIEIRA DA SILVA R -52 EFFECT OF THIOPHENACETAMIDE AGAINST MYCOBACTERIUM BOVIS (BCG) INFECTION. FATIMA MARIA FIGUEROA VERGARA, ANDRÉ LUIS PEIXOTO CANDÉA, PATRÍCIA PACHECO DA SILVA, MARCUS VINÍCIUS NORA, MARIA DAS GRAÇAS MULLER DE OLIVEIRA HENRIQUES R -53 EVALUATION OF TH1 CYTOKINE EXPRESSION DURING CEREBRAL MALARIA INFECTION IN MSG-OBESE MICE. RENAN VILLANOVA HOMEM DE CARVALHO, CHRISTIANE LIMA MACHADO, ANA GUALBERTO, SARA MALAGUTI SOARES, GABRIELA COELI MENEZES EVANGELISTA, POLLYANA SALVADOR, GILSON COSTA MACEDO, JACY GAMEIRO R -54 ULTRASTRUCTURAL STUDY OF CANDIDA ALBICANS ADHESION TO GERBIL (MERIONES UNGUICULATUS) VAGINAL AND UTERINE EPITHELIUM THALITTA HETAMARO AYALA LIMA, CAROLINA MARTINS TREMÉA, LUIZ HENRIQUE ATHAIDES RAMOS, SEBASTIÃO ROBERTO TABOGA, LÚCIA KIOKO HASIMOTO E SOUZA, FERNANDA CRISTINA ALCANTARA DOS SANTOS, JOSIANE FAGANELLO R -55 INVESTIGATION OF LIPID BODIES IN TRYPANOSOMA CRUZI AND THEIR CORRELATION WITH CHAGAS’ DISEASE DANIEL AFONSO DE MENDONÇA TOLEDO, HELOISA D’AVILA BIZARRO, LÍVIA TEIXEIRA, CÉLIO FREIRE DE LIMA, PATRÍCIA TORRES BOZZA, ROSSANA C. N. MELO R -56 ISOLATION AND CHARACTERIZATION OF GENES CODING FOR PHYTOCYSTATINS FROM BLACK PEPPER- FUSARIUM SOLANI F. SP. PIPERIS INTERACTION ALINE MEDEIROS LIMA, SÁVIO PINHO DOS REIS, WENDELL UPTON DE BRITO, LILIANE SOUZA CONCEIÇÃO TAVARES, ELAINE CRISTINA PESSOA DOS SANTOS, CLAUDIA REGINA BATISTA DE SOUZA R -57 NAPHTHOQUINONE DERIVATIVE CHEMOTHERAPY ACTION IN THE IN VITRO DEVELOPMENTAL OF TOXOPLASMA GONDII. LUCIANA LEMOS RANGEL DA SILVA, JULIANA DE ARAÚJO PORTES, SÉRGIO HENRIQUE SEABRA, RENATO AUGUSTO DAMATTA R -58 THE ROLE OF UBIQUITIN-PROTEASOME SYSTEM IN VIRAL PRODUCTION AND INFECTIVITY OF SIVCPZ MARCELA SABINO CUNHA, LUCIANA JESUS DA COSTA R -59 PI3K AND AKT SIGNAL PATHWAY ARE INVOLVED DURING INVASION OF HUMAN UMBILICAL VEIN ENDOTHELIAL CELLS BY GROUP B STREPTOCOCCUS RAFAELA SILVA DOS SANTOS, JESSICA SILVA SANTOS DE OLIVEIRA, GABRIELA DA SILVA SANTOS, PRESCILLA EMY NAGAO R -63 USING PROTEOMIC ANALYSIS TO IDENTIFY CHEMOTHERAPEUTIC AGENTS IN MACROPHAGES INFECTED WITH LEISHMANIA CARLOS EDUARDO SAMPAIO GUEDES, BEATRIZ ROCHA SIMÕES DIAS, ANTONIO LUIS DE OLIVEIRA ALMEIDA PETERSEN, KERCIA PINHEIRO CRUZ, NIARA DE JESUS ALMEIDA, JULIANA PERRONE BEZERRA DE MENEZES, LUIZ ANTÔNIO RODRIGUES DE FREITAS, PATRICIA SAMPAIO TAVARES VERAS R -64 DENGUE AND YELLOW FEVER VIRUS-MEGAKARYOBLASTS INTERACTION: ROLE IN HEMOSTATIC ALTERATIONS SAMIR PEREIRA DA COSTA CAMPOS, MARIANA GARRIDO DE CASTRO, DANIEL SANCHES, CLAUDIA MONTEIRO DA ROCHA, MARIANA FIGUEIREDO RODRIGUES, JERSON LIMA DA SILVA, ANDRE MARCO DE OLIVEIRA GOMES, ANDRÉA CHEBLE DE OLIVEIRA R -65 COMPARISON IN SILICO OF ZIP TRANSPORTERS EXTRACELLULAR DOMAINS DISTRIBUTION OF LEISHMANIA SP AND MAMMAL HOSTS. JULIA ZERLOTINI DE LUCAS, RENATA ANDRADE AVILA, IARA FREITAS LOPES, LUCIANO RIVAROLI R -66 MICROBICIDAL RESPONSE IN MACROPHAGES INFECTED WITH L. (L.) AMAZONENSIS AFTER TREATMENT WITH AQUEOUS EXTRACT FROM ROOT OF PHYSALIS ANGULATA BRUNO JOSÉ MARTINS DA SILVA, RAQUEL RAICK PEREIRA DA SILVA, ANA PAULA DRUMMOND RODRIGUES, LUIS HENRIQUE SEABRA DE FARIAS, CAROLINE MARTINS ALMEIDA, PAULA CRISTINA RODRIGUES FRADE, GILMARA DE NAZARETH TAVARES BASTOS, EDILENE OLIVEIRA DA SILVA R -67 STUDY OF THE ROLE OF DIPEPTIDYL-PEPTIDASE 8-LIKE AND OTUBAIN GENES IN TRYPANOSOMA CRUZI INFECTION DEBORA TORRES ALVES FIGUEIREDO, FLAVIA NADDER MOTTA ARENAS, GRAZIELLA SANTANA FEITOSA FIGUEIREDO, JAIME MARTINS DE SANTANA, IZABELA MARQUES DOURADO BASTOS R -68 INFLAMMASOME ACTIVATION NEGATIVELY REGULATES LIPID BODY BIOGENESIS DURING MYCOBACTERIUM BOVIS BCG INFECTION CARLA FREITAS, DARIO ZAMBONI, VALÉRIE QUESNIAUX, BERNHARD RYFFEL, PATRICIA BOZZA R -69 THE ROLE OF SCAVENGER RECEPTOR MARCO IN INFECTION OF MURINE MACROPHAGES WITH LEISHMANIA MAJOR NIARA DE JESUS ALMEIDA, CARLOS EDUARDO SAMPAIO GUEDES, BEATRIZ ROCHA SIMÕES DIAS, PATRÍCIA SAMPAIO TAVARES VERAS R -70 HOST CELL SIGNALING PATHWAYS INVOLVED IN INVASION OF ENTEROINVASIVE ESCHERICHIA COLI (EIEC) AND SHIGELLA FLEXNERI PATRÍCIA FARIA PRADO, FLÁVIA ALVES MARTINS, LUCAS GONÇALVES FERREIRA, MARINA BAQUERIZO MARTINEZ, MARIA APARECIDA DE SOUZA, CLAUDIO VIEIRA DA SILVA R -71 EVALUATION OF TRANSLOCATOR PROTEIN EXPRESSION IN CBA MACROPHAGES INFECTED WITH LEISHMANIA BEATRIZ ROCHA SIMÕES DIAS, CARLOS EDUARDO SAMPAIO GUEDES, KERCIA PINHEIRO CRUZ, NIARA DE JESUS ALMEIDA, PATRICIA SAMPAIO TAVARES VERAS R -72 DETECTION AND IMMUNOLOCALIZATION OF THE ENZYME CONSTITUTIVE NITRIC OXIDE SYNTHASIS (CNOS) IN PROMASTIGOTES FORMS OF THE LEISHMANIA (VIANNIA) BRAZILIENSIS AND LEISHMANIA (LEISHMANIA) AMAZONENSIS RODRIGO RIBEIRO FURTADO, ANA PAULA DRUMMOND RODRIGUES, LUIS HENRIQUE SEABRA DE FARIAS, AMANDA ANASTÁCIA PINTO HAGE, CAROLINE MARTINS ALMEIDA, BRUNO JOSÉ MARTINS SILVA, EDILENE OLIVEIRA DA SILVA R -73 ULTRASTRUCTURAL DIFFERENCES IN THE ENGULFMENT PROCESS OF THREE LEISHMANIA SPECIES BY MACROPHAGE AND MICROGLIA JOSÉ ANTONIO PICANÇO DINIZ JUNIOR, PATRÍCIA KARLA SANTOS RAMOS, MAYSA DE VASCONCELOS BRITO R -74 COULD SEROTONIN LEVELS IN THE INTESTINE MANAGE THE CHAGASIC MEGACOLON DEVELOPMENT? FERNANDA CHAVES OLIVEIRA, ENIO CHAVES OLIVEIRA, SALUSTIANO GABRIEL NETO, ALEJANDRO OSTEMAIER LUQUETTI, AXEL BREHMER, ALEXANDRE BARCELOS MORAIS DA SILVEIRA, MICHELLE APARECIDA RIBEIRO DE FREITAS R -75 IN SILICO STRUCTURAL ANALYSIS OF EXTRACELLULAR DOMAIN OF TRYPANOSOMA BRUCEI TRANSFERRIN RECEPTOR RENATA ANDRADE AVILA, JULIA ZERLOTINI DE LUCAS, IARA FREITAS LOPES, LUCIANO RIVAROLI R -60 CELLULAR STUDIES OF A HISTONE DESACETYLASES INHIBITOR ON LEISHMANIA AMAZONENSIS BRUNNO RENATO FARIAS VERÇOZA, JULIANY COLA FERNANDES RODRIGUES, WANDERLEY DE SOUZA, FRANZ BRACHER R -61 MECHANISM OF INTERACTION BETWEEN PHAGOSOMES AND RAB 7-ENRICHED LIPID BODIES DURING MYCOBACTERIAL INFECTION NATALIA R 116 S – Methods in Cell Biology S1-S30 S-1 COMPARATIVE HISTOCHEMICAL ANALYSIS OF THE VENOM GLANDS OF THE BEE, AFRICANIZED APIS MELLIFERA AND THE WASP, POLISTES VERSICOLOR. ALINE FERNANDA CATAE, THAISA CRISTINA ROAT, MARIO SÉRGIO PALMA, ROBERTA CORNÉLIO FERREIRA NOCELLI, OSMAR MALASPINA CULTURES. LEANDRA SANTOS BAPTISTA, KARINA RIBEIRO SILVA, CAROLINA DA SILVA GOUVEIA PEDROSA, RONALDO JOSÉ FARIA CORREIA DO AMARAL, JOÃO VITOR BELIZÁRIO DOS SANTOS, RADOVAN BOROJEVIC, JOSÉ MAURO GRANJEIRO S - 16 OPTICAL TWEEZERS TO EVALUATE THE LEISHMANIA AMAZONENSIS AND TRYPANOSOMA CRUZI MOTILITY BEFORE AND AFTER TREATMENT WITH LIPPIA SIDOIDES ESSENTIAL OIL ALINE DULCE PITT DA ROCHA OLIVEIRA, DIEGO CÉSAR NUNES DA SILVA, ANA CAROLINA SANTOS ROSA NUNES, ALINE CAROLINE DA SILVA, AMANDA SILVA DOS SANTOS ALIANÇA, DIVAR FERNANDES PIRES NETO, ANDREZA RAPOSO BORGES, BEATE SAEGESSER DOS SANTOS, REGINA CÉLIA BRESSAN QUEIROZ DE FIGUEIREDO, ADRIANA FONTES S - 17 HISTOLOGICAL EVALUATION OF BIOMATERIAL CHITOSANGELATIN-BASED EFFECTS IN INTRAORAL BONE REPAIR IN RATS IGOR DANIEL GARCIA REIS, MATHEUS HENRIQUE SANTOS ASSIS, LUIZ BERTOLDO DA COSTA FILHO, FERNANDO ANTÔNIO MAUAD ABREU, PETERSON DE OLIVEIRA DUTRA, ALFREDO MIRANDA GÓES, GERLUZA APARECIDA BORGES SILVA S-2 NUCLEAR MORPHOMETRIC ANALYSIS (NMA): A NEW METHOD FOR SCREENING OF APOPTOSIS, MITOSIS, SENESCENCE AND MITOTIC CATASTROPHE EDUARDO CREMONESE FILIPPI CHIELA, MANUEL MENEZES DE OLIVEIRA NETO, BRUNO JURKOVSKI, SÍDIA MARIA JACQUES-CALLEGARI, VINÍCIUS DUVAL DA SILVA, GUIDO LENZ S - 18 PREDICTION OF AUTOPHAGY IN VITRO BY A COLORIMETRIC APPROACH WALESKA KERLLEN MARTINS GARDESANI, DIVINOMAR SEVERINO, CLEIDIANE SOUZA, BEATRIZ SIMONSEN STOLF, MAURÍCIO SILVA BAPTISTA S-3 SECRETION SYNTHESIS BY VENOM RESERVOIR OF WASP TRYPOXYLON LACTITARSE SAUSSURE (HYMENOPTERA: CRABRONIDAE) JÔNATAS CHAGAS DE OLIVEIRA, RUSLEYD MARIA MAGALHÃES DE ABREU S - 19 FLUORESCENCE PLATE READER TO EVALUATE CDTE/CDS-MPA AND CDTE/CDS-MSA BIOCONJUGATION TO ANTI-A ANTIBODIES – APPLICATIONS IN IMMUNOHEMATOLOGY PAULO EUZEBIO CABRAL FILHO, KILMARA HIGIA GOMES CARVALHO, ALUIZIO GONÇALVES BRASIL JUNIOR, ELISA SOARES LEITE, BEATE SAEGESSER SANTOS, ADRIANA FONTES S-4 STANDARDIZATION OF TOTAL DNA QUANTIFICATION AND DETERMINATION OF AT/CG BASE PAIRS COMPOSITION METHODOLOGIES FOR STINGLESS BEES, BY MEANS OF FLOW CYTOMETRY FERNANDA APARECIDA FERRARI SOARES, CARLOS ROBERTO CARVALHO, MARA GARCIA TAVARES S-5 DIFFERENTIAL HSP 70 AND HYPOXIA-INDUCIBLE FACTOR 1A (HIF1A) EXPRESSION IN LIVER OF PROCHILODUS ARGENTEUS CAUGHT IN DIFFERENT SITES OF SÃO FRANCISCO RIVER HEDER JOSÉ RIBEIRO, MARCELA SANTOS PROCÓPIO, FABIANA ALVES, DEBORAH RIBEIRO NASCIMENTO, ALMIR DE SOUSA MARTINS, JOSÉ DIAS CORRÊA JUNIOR S-6 CDTE/CDS QUANTUM DOTS BIOCONJUGATED TO CONCANAVALIN A LECTIN TO STUDY CARBOHYDRATES EXPRESSION IN CANDIDA ALBICANS DENISE PATRÍCIA LINS AZEVEDO TENÓRIO, CAMILA GALVÃO DE ANDRADE, PAULO EUZÉBIO CABRAL FILHO, CAMILA CAMPOS SANTOS, ILKA TIEMY KATO, MARTHA SIMÕES RIBEIRO, SEVERINO ALVES JUNIOR, EDUARDO ISIDORO CARNEIRO BELTRÃO, LUIZ BEZERRA DE CARVALHO JUNIOR, ADRIANA FONTES, BEATE SAEGESSER SANTOS S-7 IDENTIFICATION OF PARAPOXVIRUS ISOLATED FROM AN OUTBREAK IN GOATS IN CEARÁ STATE, BRAZIL, BY TRANSMISSION ELECTRON MICROSCOPY TECHNIQUES. MARCIA HELENA BRAGA CATROXO, ANA MARIA CRISTINA RABELO PINTO DA FONSECA MARTINS, SELMA PETRELLA, FABÍOLA DE SOUZA, BEATRIZ DI BOARETO NASTARI, RODRIGO BARBOSA DE SOUZA S-8 MEMBRANE NANOTUBES: MECHANISMS OF FORMATION AND FUNCTIONS IN CELLS BRUNO PONTES, NATHAN BESSA VIANA, YARENI AYALA, ANNA CAROLINA CARVALHO DA FONSECA, LUCIANA FERREIRA ROMÃO, RACKELE FERREIRA AMARAL, LEONARDO TAVARES SALGADO, FLÁVIA REGINA DE SOUZA LIMA, LORAINE CAMPANATI, MARCOS FARINA, VIVALDO MOURA NETO, H. MOYSES NUSSENZVEIG S-9 MORPHOLOGICAL AND ULTRASTRUCTURAL STUDY OF SALIVARY GLAND OF TRIATOMA INFESTANS (HEMIPTERA, TRIATOMINAE) NADJA BIONDINE MARRIEL, PAULO FILEMON PAOLUCCI PIMENTA, ANA CAROLINA BORELLA ANHÊ S - 10 EVALUATION OF THE TOXICITY OF THALIDOMIDE INCORPORATED IN BIODEGRADABLE IMPLANTS PEDRO ALCANTARA FONSECA DE SOUZA, SÍLVIA LIGÓRIO FIALHO, ARMANDO SILVA-CUNHA, LUCIANA MARIA SILVA S - 11 IDENTIFICATION AND EVALUATION OF BIOLOGICAL CONSERVATION OF DIFFERENT TOXINS FROM BROWN SPIDER VENOM (LOXOSCELES GENUS) IN THREE SPECIES OF GREATER IMPACT ON PARANÁ: L. INTERMEDIA, L. LAETA AND L. GAUCHO FERNANDA NUNES SOUZA, DANIELA REGINA BUCH, GABRIEL OTTO MEISSNER, DILZA TREVISAN SILVA, MÁRCIA HELENA APPEL, RAFAEL BERTONI DA SILVEIRA, DANIELE CHAVES MOREIRA, LUIZA HELENA GREMSKI, ANDREA SENFF-RIBEIRO, OLGA MEIRI CHAIM, SILVIO SANCHES VEIGA S - 12 CONSTRUCTION OF A CDNA LIBRARY FOR ANTIMICROBIAL PEPTIDES FROM HYPSIBOAS SEMILINEATUS LORENA NACIF MARÇAL, MONISE VIANA ABRANCHES, GRACIELLE RODRIGUES PEREIRA, NATÁLIA CRISTINA SANTOS COSTA, HELIOMAR CAZELLI DE OLIVEIRA FILHO, RENATO NEVES FEIO, EDUARDO RESENDE HONDA, SÉRGIO OLIVEIRA DE PAULA, LEANDRO LICURSI DE OLIVEIRA S - 13 DEVELOPMENT OF A BIOENGINEERED HUMAN-SKIN CHICK EMBRYO MODEL FOR TESTING DRUGS FOR SKIN INFLAMMATORY DISORDERS KAREN JACKSON, ROTEM BEN SHUSHAN, HILA YEHUDA, LEONID KOGAN, SNAIT TAMIR S - 14 ATOMIC FORCE MICROSCOPY AS A TOOL TO STUDY THE MITOCHONDRIAL DNA OF TRYPANOSOMATIDS MARCELO ZOGOVICH, DANIELA LEÃO GONÇALVES, LILIAN TEREZINHA COSTA, WANDERLEY DE SOUZA, DANIELLE PEREIRA CAVALCANTI S - 15 UNRAVELING OPTIMIZED CONDITIONS FOR CARTILAGE TISSUE ENGINEERING USING THREE-DIMENSIONAL HIGH-DENSITY HUMAN STEM CELL S - 20 EVALUATION OF DIFFERENT METHODOLOGIES TO ACHIEVE EFFICIENT EXPRESSION OF THE DENGUE VIRUS NS1 PROTEIN IN HEPG2 CELLS KÍSSILA RABELO, EDSON ROBERTO ALVES DE OLIVEIRA, ADA MARIA DE BARCELOS ALVES, SIMONE MORAIS DA COSTA S - 21 MEASUREMENT OF PROTEIN DISULFIDE ISOMERASE REDUCTASE ACTIVITY IN BIOLOGICAL SAMPLES BY INSULIN ASSAY DENISE DE CASTRO FERNANDES, MONICA MASSAKO WATANABE, FRANCISCO RAFAEL MARTINS LAURINDO S - 22 OBTENTION OF 3D SPHEROIDAL CULTURE FROM MC3T3-E1 MURINE PRE-OSTEOBLASTIC CELLS FOR BIOCOMPATIBILITY STUDIES EMANUELLE STELLET LOURENÇO, ROBER FREITAS BACHINSKI, ADRIANA BRANDÃO RIBEIRO LINHARES, JOSÉ MAURO GRANJEIRO, GUTEMBERG GOMES ALVES S - 23 PREDICTION OF ACUTE TOXICITY OF A MEDICINAL PLANT CASEARIA SYLVESTRIS FLUID EXTRACT BY IN VITRO MODEL ALINE ZANCHETI AMENI, ANDREA CECILIA DORION RODAS, SILVANA LIMA GÓRNIAK S - 24 CRYO-SCANNING ELECTRON MICROSCOPY FOR THE ADVANCED STUDY OF CRYO-FIXED AND HYDRATED SAMPLES. RENATA TRAVASSOS, EDUARDO JOSÉ LOPES TORRES, KILDARE MIRANDA, MÁRCIA ATTIAS S - 25 BACTERIAL CELL CULTURE IMPROVEMENT AIMING THE PRODUCTION OF CISTINE KNOT FAMILY PEPTIDE FROM BROWN SPIDER (LOXOSCELES INTERMEDIA) VENOM GABRIEL OTTO MEISSNER, FENADO H. MATSUBARA, ALINE VIANA BEDNASKI, EDUARDO MENDONÇA, LUCAS PEDROSA, LUIZA HELENA GREMSKI, SILVIO SANCHES VEIGA, OLGA MEIRI CHAIM S - 26 IMMUNE CELL CULTURE IN TROPICAL SEA URCHIN LYTECHINUS VARIEGATUS PAOLA CRISTINA BRANCO, DOUGLAS AMARAL DOS SANTOS, DÉBORA ALVARES LEITE FIGUEIREDO, JOSÉ ROBERTO MACHADO CUNHA DA SILVA S - 27 STANDARDIZATION OF COMET ASSAY WITH DAPHNIA MAGNA STRAUS (1820) FERNANDA FLEIG ZENKNER, CAMILA GONÇALVES ATHANÁSIO, JOEL HENRIQUE ELLWANGER, DANIEL PRÁ, EDUARDO ALEXIS LOBO, ALEXANDRE RIEGER S - 28 EVALUATION OF THE IN VIVO CROSS LINKING METHOD FOR LEISHMANIA BRAZILIENSIS TO OBTAIN COMPLEXES WITH OTUBAIN, A DEUBIQUITYLATING ENZYME CLENIA DOS SANTOS AZEVEDO, JULIANA ARAUJO CARNEIRO, JHONATA LIMA PEREIRA, FLAVIA NADER MOTTA ARENAS, JAIME MARTINS DE SANTANA, IZABELA MARQUES DOURADO BASTOS S - 29 CORRELATIVE MICROSCOPY WITH ‘SHUTTLE & FIND’ – COMBINING THE POWER OF IMAGING DEVICES. JENS MARKUS RIETDORF S - 30 ULTRASTRUCTURAL ANALYSIS OF THE MYCELIUM OF DEMATIACEOUS MOLD CURVULARIA SP UNDER THE ACTION OF THE ANTIFUNGAL AGENT AMPHOTERICIN B ADRIANO BIANCALANA, FERNANDA SIMAS CORRÊA BIANCALANA, LUZIA LYRA, ANGÉLICA ZANINELLI SCREIBER T – Neurobiology T1-T85 T-1 EFFECTS OF VENOUS ANESTHETIC ETOMIDATE ON SYNAPTIC VESICLE EXOCYTOSIS AT THE MICE NEUROMUSCULAR JUNCTION PRISCILA APARECIDA COSTA VALADÃO, CRISTINA GUATIMOSIM FONSECA, JANICE HENRIQUES DA SILVA, RENATO SANTIAGO GOMEZ 117 T-2 OPTICAL AND ULTRASTRUCTURAL ANALYSIS OF NEUROMUSCULAR JUNCTIONS FROM DIAPHRAGMS OF MICE WITH CHOLINERGIC DYSFUNCTION HERMANN ALECSANDRO RODRIGUES, MATHEUS DE CASTRO FONSECA, PATRÍCIA MASSARA MARTINELLI, PATRÍCIA MARIA D'ALMEIDA LIMA, VÂNIA FERREIRA PRADO, MARCO ANTÔNIO MÁXIMO PRADO, CRISTINA GUATIMOSIM FONSECA T-3 EFFECTS OF INHALATORY ANESTHETIC SEVOFLURANE ON SYNAPTIC VESICLE EXOCYTOSIS AT THE MOUSE NEUROMUSCULAR JUNCTION MATHEUS DE CASTRO FONSECA, JANICE HENRIQUES DA SILVA, RENATO SANTIAGO GOMEZ, CRISTINA GUATIMOSIM T-4 EFFECT OF UVA AND UVB ON THE EXPRESSION OF SEROTONIN IN THE CENTRAL OLFACTORY SYSTEM OF THE CRAB UCIDES CORDATUS. GABRIELLE DE JESUS FERREIRA, GABRIELA HOLLMANN, ÁLVARO LEITÃO, SILVANA ALLODI T-5 INCREASE IN PROPORTION OF SUBSTANCE P NERVE FIBERS IN INDIVIDUALS WITH CHAGASIC MEGAESOPHAGUS RODOLFO DUARTE NASCIMENTO, PATRÍCIA ROCHA MARTINS, JACQUELINE GARCIA DUARTE, DÉBORA D’ÀVILA REIS T-6 RELATION BETWEEN THE AREA OF GFAP ENTERIC GLIAL CELLS AND THE NUMBER OF NEURONS IN ESOPHAGUS OF CHAGASIC INDIVIDUALS RODOLFO DUARTE NASCIMENTO, PATRÍCIA ROCHA MARTINS, JACQUELINE GARCIA DUARTE, DÉBORA D’ÀVILA REIS T-7 LOSS OF INTERSTITIIAL CELLS OF CAJAL MIGHT PRECEED DENERVATION PROCESS IN CHRONIC TRYPANOSOMA CRUZI INFECTION PATRÍCIA ROCHA MARTINS, RODOLFO DUARTE NASCIMENTO, JACQUELINE GARCIA DUARTE, SHEILA ADAD, DÉBORA D’ÀVILA REIS T-8 DENDRITIC SPINES IN THE MEPD OF RATS: MORPHOLOGY AND CONNECTIVITY DALPIAN, F., BRUSCO, J., MOREIRA J.E., RASIA-FILHO, A. T-9 ATP CONTROLS PROLIFERATION IN VIVO OF RAT RETINAL PROGENITORS LUANA DE ALMEIDA PEREIRA, ALFRED FRANCO SHOLL, ANA LUCIA MARQUES VENTURA, LUCIANNE FRAGEL MADEIRA T - 10 MOLECULAR TOOLS TO ANALISYS OF HUNTINGTON’S DISEASE HAPLOTYPES (CAG/CCG TRINUCLEOTIDE REPEATS) ON HTT GENE IN BRAZILIAN PATIENTS LUCIANA DE ANDRADE AGOSTINHO, CATIELLY FERREIRA ROCHA, ENRIQUE MEDINA-ACOSTA, HAZEL NUNES BARBOZA, ANTONIO FRANCISCO ALVES DA SILVA, SIMÃO PEDRO FERNANDES PEREIRA, EDUARDO RIBEIRO PARADELA, ANDRÉ LUIS DOS SANTOS FIGUEIREDO, EDUARDO DE MATOS NOGUEIRA, REGINA MARIA PAPAIS ALVARENGA, SUELY RODRIGUES DOS SANTOS, CARMEN LUCIA ANTÃO PAIVA T - 11 VIRAL ENCEPHALITIS INDUCED BY DENGUE VIRUS IN ALBINO SWISS MICE: THE INFLAMMATORY RESPONSE IN NEONATE’S NERVOUS SYSTEM GIOVANNI FREITAS GOMES, MAIRA CATHERINE PEREIRA TURIEL, CÉSAR AUGUSTO RAIOL FORO, BRUNNO GOMES PINHO, CARLA MAISA DAMASCENO REGO, MARINA CUTRIM MAGALHAES, PEDRO FERNANDO DA COSTA VASCONCELOS, CRISTOVAM WANDERLEY PICANÇO DINIZ T - 12 PARKINSON`S DISEASE AND CEREBELLUM: NEW WAY OF ANALYZING THE BRAIN LESIONS BY A PARAQUAT-INDUCED CLASSICAL EXPERIMENTAL MODEL JOEL HENRIQUE ELLWANGER, FERNANDA FLEIG ZENKNER, JULIANO ASSMANN, DANIEL PRÁ, MICHELE GASSEN KELLERMANN, ALEXANDRE RIEGER, JOÃO ANTONIO PÊGAS HENRIQUES, DEIVIS DE CAMPOS T - 13 EXPRESSION OF ALPHA SYNUCLEIN AND HIPERPHOSPHORYLATION OF TAU IN THE BRAIN OF AGED RATS EXPOSED TO LOW CONCENTRATIONS OF ROTENONE CAROLLINY MOURA DA SILVA, MICHAEL FERNANDES DE ALMEIDA, MERARI DE FÁTIMA RAMIRES FERRARI T - 14 EFFECT OF PROPOFOL ON ACETYLCHOLINE RELEASE IN RAT HIPPOCAMPUS SYNAPTOSOMES FLÁVIA LAGE PESSOA DA COSTA, NANCY SCARDUA BINDA, LUANNA DA SILVA MONTEIRO, RENATO SANTIAGO GOMEZ, MARCUS VINÍCIUS GOMEZ T - 15 SYNAPTIC CHANGES IN THE CORTEX OF INDIVIDUALS WITH MESIAL TEMPORAL LOBE EPILEPSY ALONG AGING: LIPOFUSCIN GRANULES AS MARKERS OF AGE SUÉLEN MERLO, ANA BEATRIZ NAKAYAMA, JANAINA BRUSCO, MARCOS ANTÔNIO ROSSI, CARLOS GILBERTO CARLOTTI JÚNIOR, JORGE EDUARDO MOREIRA T - 16 NEUROGENESIS AND SYNAPTIC PLASTICITY IN RATS SUBMITTED TO MATERNAL SEPARATION AND ENRICHED ENVIRONMENT SUÉLEN MERLO, JOSÉ INÁCIO LEMOS, LENALDO BRANCO ROCHA, MARCOS ANTÔNIO ROSSI, JORGE EDUARDO MOREIRA T - 17 CONSTANT EXPRESSION OF ALFA-SINUCLEIN AND UBIQUITIN ALONG AGING IN THE CORTEX OF INDIVIDUALS WITH MESIAL TEMPORAL LOBE EPILEPSY ANA BEATRIZ SOUZA NAKAYAMA, SUÉLEN MERLO, JANAÍNA BRUSCO, MARCOS ANTÔNIO ROSSI, CARLOS GILBERTO CARLOTTI JR, JORGE EDUARDO MOREIRA T - 18 MORPHOMETRIC ANALYSIS OF SOLEUS AND TRICEPS BRACHII SKELETAL MUSCLES OF MICE WITH CHOLINERGIC DYSFUNCTION MATHEUS PROENÇA SIMÃO MAGALHÃES GOMES, HERMANN ALECSANDRO RODRIGUES, PATRÍCIA MASSARA MARTINELLI, VANIA FERREIRA PRADO, MARCO ANTÔNIO MÁXIMO PRADO, CRISTINA GUATIMOSIM FONSECA T - 19 EFFECT OF CONDITIONED MEDIUM FROM CULTURES OF OLFACTORY ENSHEATHING GLIA ON HIPPOCAMPAL NEURAL CELLS IN VITRO. LITIA ALVES DE CARVALHO, ROBERTA PEREIRA DE MELO GUIMARÃES, RICARDO AUGUSTO DE MELO REIS, LENY ALVES CAVALCANTE T - 20 FOOD RESTRICTION DOES NOT INTERFERE IN AGE-RELATED LOSS OF GLIA AND CHOLINERGIC MYENTERIC NEURONS JOÃO PAULO FERREIRA SCHOFFEN, JONATAS DE PAULA OLIVEIRA, ANA PAULA DE SANTI RAMPAZZO, CARLA POSSANI CIRILO, MARIANA CRISTINA VICENTE UMADA ZAPATER, FERNANDO AUGUSTO VICENTINI, ANACHARIS BABETO DE SÁ-NAKANISHI, JURANDIR FERNANDO COMAR, MARIA RAQUEL MARÇAL NATALI T - 21 IMPOVERISHED ENVIRONMENT AND REDUCED MASTICATORY ACTIVITY AGGRAVATE AGING SPATIAL MEMORY DECLINE IN ALBINO SWISS MICE ALBERT LUIZ COSTA DA COSTA, FABÍOLA DE CARVALHO CHAVES DE SIQUEIRA MENDES, MARINA NEGRÃO FROTA DE ALMEIDA, ANDRÉ PINHEIRO GURGEL FELÍCIO, MANOELA FALSONI, MARCIA LORENA FERREIRA DE ANDRADE;, JOÃO BENTO TORRES NETO;, CRISTOVAM WANDERLEY PICANÇO-DINIZ;, MARCIA CONSENTINO KRONKA SOSTHENES. T - 22 WALLERIAN DEGENERATION IN GALECTIN-3 KNOCKOUT MICE BRUNO DE SIQUEIRA MIETTO, SOFIA JURGENSEN HARTKE, LUCINÉIA ALVES, MARCELO SAMPAIO NARCISO, IRANAIA ASSUNÇÃO MIRANDA, DEA MARIA SERRA VILLA-VERDE, FLÁVIA REGINA SOUZA LIMA, MARCELO TORRES BOZZA, ANA MARIA BLANCO MARTINEZ T - 23 ACTIVATION OF AKT1 PROTEIN BY ALLOSTERIC MODULATORS OF GLUTAMATE METABOTROPIC RECEPTOR 5 IN PRIMARY CULTURE OF STRIATAL NEURONS FLAVIA RODRIGUES SILVA, FABIOLA MARA RIBEIRO, JULIANA GUIMARÃES DÓRIA, JÉSSICA MABELLE DE SOUZA, HELTON JOSÉ DOS REIS, TOMAS DOBRANSKY T - 24 HYPOTHALAMIC AND CORTICAL ASTROCYTES RESPOND DIFFERENTLY TO THE ADDITION OF FATTY ACID IN VITRO ÉRICA VIEIRA DOS SANTOS, PEDRO AUGUSTO SILVA NOGUEIRA, RENATA GRACIELE ZANON T - 25 OPTICAL ANALYSIS OF NEUROMUSCULAR JUNCTIONS OF DIAPHRAGM MUSCLE FROM A TRANSGENIC MICE MODEL FOR HUNTINGTON’S DISEASE BÁRBARA CAMPOS DE ARAGÃO, HERMANN ALECSANDRO RODRIGUES, FABÍOLA MARA RIBEIRO, CRISTINA GUATIMOSIM FONSECA T - 26 MASTICATORY REHABILITATION IMPROVES MICE EPISODIC-LIKE MEMORY ANA CARLA FADEL, ANDRÉ PINHEIRO GURGEL FELÍCIO, FABÍOLA DE CARVALHO CHAVES DE SIQUEIRA MENDES, RAÍSSA AIRES RIBEIRO BRINGEL, RODRIGO PEREZ DA SILVA, KÁTIA DE AVIZ FONSECA, JOSÉ FERNANDO CARNEIRO JUNIOR, ANTÔNIO LUIZ BREIA DA SILVA JUNIOR, CRISTOVAM WANDERLEY PICANÇO DINIZ, MARCIA CONSENTINO KRONKA SOSTHENES T - 27 ASPECTS OF DEGENERATION AND REGENERATION OF THE STYELA PLICATA NEURAL COMPLEX INDUCED BY 3-ACETYLPYRIDINE BIANCA NICOLE SANTOS PAEZ MEDINA, ISADORA SANTOS DE ABREU, SILVANA ALLODI, RODRIGO NUNES DA FONSECA, CÍNTIA MONTEIRO DE BARROS T - 28 ASSESSMENT CELLULAR AFTER SPINAL CORD INJURY IN RAT: CORRELATION WITH FUNCTIONAL RECOVERY ANDRÉA MARINS DAMASCENO BOMFIM, JASON ROBBERT POTAS, NEWTON GONÇALVES DE CASTRO, ROSÁLIA MENDEZ-OTERO T - 29 DECREASE OF AUTOPHAGY AFTER ROTENONE EXPOSURE IN CULTURED CELLS. LUANA DE SANTANA BOTTAS, MERARI DE FÁTIMA RAMIRES FERRARI T - 30 EFFECT OF CHRONIC ADMINISTRATION OF CAFFEINE ON MAP2 AND SYNAPTOPHYSIN IN HIPPOCAMPUS OF MALE AND FEMALE RATS LETICIA FERREIRA PETTENUZZO, CARINA DE SOUZA MOTA, CARLA DALMAZ T - 31 SICKNESS BEHAVIOR AND MICROGLIAL CHANGES IN THE DENTATE GYRUS OF ADULT MICE (MUS MUSCULUS) AFTER INJECTION OF LIPOPOLYSACCHARIDE (LPS) OF SALMONELLA ENTERIC THAIS BARROSO NAVES, ANA CARLA FADEL, DANIEL GUERREIRO DINIZ, GIOVANNI FREITAS GOMES, GRAZIELLA DE ASSIS MALERBA, ANA MARIA MATOS DE OLIVEIRA ROSSY, BRUNA DO SOCORRO AMORIM DE LIMA, CARMEN DOS SANTOS FERNANDES, EDIANE BARROS DA SILVA, EDUARDO AUGUSTO CRUZ DA SILVA, LUCIANO DE SENA ARAUJO, MARIA DAS GRAÇAS REIS, ROSIGLEIDE GOMES DOS SANTOS, SUZANE SANTOS CORREA, ZAIRA MONIK NUNES BARROS, CRISTOVAM WANDERLEY PICANÇO DINIZ, CRISTOVAM WANDERLEY PICANÇO DINIZ T - 32 INVESTIGATION OF THE METABOTROPIC RECEPTOR 5 ROLE IN HYPERKINESIS USING A MICE MODEL OF HUNTINGTON’S DISEASE ISABELLA MONTEIRO GUIMARÃES, FABIOLA MARA RIBEIRO, RITA G. W. PIRES, TOMAS DOBRANSKY T - 33 PRION PROTEIN MUTATIONS ASSOCIATED WITH PRION DISEASES IMPAIR DIFFERENTIATION INDUCED BY LAMININ CLEITON FAGUNDES MACHADO, FLAVIO H. BERALDO, TIAGO G. SANTOS, DOMINIQUE BOURGEON, MICHELE C. LANDEMBERGER, VILMA R. MARTINS T - 34 FLAVONOIDS PROMOTES NEURONAL SURVIVAL AND SYNAPTOGENESIS IN CEREBRAL CORTEX IN VITRO ISADORA CRISTINA PEREIRA MATIAS, JOICE STIPURSKY, FLÁVIA CARVALHO ALCÂNTARA GOMES 118 T - 35 STUDY OF PROTEIN CARBONYLS IN AGED RATS EXPOSED TO LOW DOSE OF ROTENONE MICHAEL FERNANDES DE ALMEIDA, CAROLLINY MOURA DA SILVA, MERARI DE FÁTIMA RAMIRES FERRARI MULTIVESICULAR BODIES HIANARA BUSTAMANTE, ANDRES RIVERA-DICTTER, VIVIANA CAVIERES, ALEXIS GONZALEZ, VANESSA MUÑOZ, JUAN S. BONIFACINO, GONZALO A. MARDONES, PATRICIA V. BURGOS T - 36 SECRETION OF STRESS INSDUCIBLE PROTEIN 1 IN MICROVESICLES BY ASTROCYTES GLAUCIA HAJJ, CAMILA ARANTES, MARCOS S. DIAS, ISABEL PORTO-CARREIRO, MARTÍN ROFFÉ, MARCO M. A. PRADO, RAFAEL LINDEN, VILMA R. MARTINS T - 53 BLOOD-BRAIN BARRIER BREAKDOWN AND REPAIR FOLLOWING GLIOTOXIC DRUG INJECTION IN THE BRAINSTEM OF STREPTOZOTOCIN-DIABETIC RATS MARIA DE FÁTIMA MONTEIRO MARTINS, EDUARDO FERNANDES BONDAN T - 37 IGF-I-INDUCED MODULATION OF THE (NA/K)-ATPASE ACTIVITY DURING THE POSTNATAL DEVELOPMENT OF RAT RETINA SHEILA MATURANA TEIXEIRA, ELIZABETH GIESTAL DE ARAUJO, LUIZ ROBERTO LEÃO FERREIRA T - 38 MORPHOLOGICAL CHARACTERIZATION OF CARDIAC TISSUE FROM A MOUSE MODEL OF CHOLINERGIC DYSFUNCTION MARINA VIVEIROS TRAJANO CRUZ, HERMANN ALECSANDRO RODRIGUES, PATRÍCIA MASSARA MARTINELLI, MARCO ANTÔNIO PRADO, VÂNIA FERREIRA PRADO, SILVIA GUATIMOSIM, CRISTINA GUATIMOSIM T - 39 LAMININ-Γ1 CHAIN AND STRESS INDUCIBLE PROTEIN 1 SYNERGISTICALLY MEDIATE PRPC-DEPENDENT AXONAL GROWTH VIA CA2+ MOBILIZATION IN DORSAL ROOT GANGLIA NEURONS TIAGO GOSS DOS SANTOS, FLAVIO H BERALDO, GLAUCIA NM HAJJ, MARILENE H LOPES, FERNANDA CS LUPINACCI, MARCO AM PRADO, VILMA R MARTINS T - 40 SHIITAKE MUSHROOM (LENTINULA EDODES) DOES NOT CAUSE COGNITIVE IMPAIRMENTS, BUT INDUCES DNA DAMAGE IN HIPPOCAMPAL CELLS IN RATS PATRÍCIA MOLZ, JOEL HENRIQUE ELLWANGER, FERNANDA FLEIG ZENKNER, MORGANA TONET MENDONÇA, DEIVIS DE CAMPOS, MARISA TEREZINHA LOPES PUTZKE, DANIEL PRÁ, SILVIA ISABEL RECH FRANKE T - 41 THE BRIGHT SIDE OF PRION PROTEIN IN ALZHEIMER’ DISEASE: THE ROLE OF STRESS INDUCIBLE PROTEIN 1 AS A NEUROPROTECTIVE FACTOR. BIANCA LUISE TEIXEIRA, PEDRO HIRATA, ANA PAULA SAMPAIO, JORDANO BRITOMOREIRA, SERGIO FERREIRA, MARCO PRADO, GLAUCIA HAJJ, VILMA R. MARTINS T - 42 HIPPOCAMPAL CELL CULTURE EXPOSED TO LOW DOSES OF ROTENONE EXHIBITED PROTEASOMAL ACTIVITY INHIBITION AND TAU HYPERPHOSPHORYLATION IN ABSENCE OF PROTEIN CARBONYLATION. RODRIGO DOS SANTOS CHAVES, MARILENE DEMASI, MERARI DE FATIMA RAMIRES FERRARI T - 43 HIGH-FAT DIET CAUSES ENTERIC NEURONAL LOSS IN THE DISTAL COLON OF MICE. EVANDRO JOSÉ BERALDI, LIA MARA TEOBALDO TIRONI, ANGÉLICA SOARES, ROBERTO BARBOSA BAZOTTE, NILZA CRISTINA BUTTOW T - 44 INCREASED HIPPOCAMPAL GFAP IN YOUNG RATS FROM ANIMAL MODEL OF AUTISM INDUCED BY PRENATAL EXPOSURE TO VALPROIC ACID ROBERTA BRISTOT SILVESTRIN, GIOVANA BROLESE, CRISTIANE BATASSINI, MÁRCIO FERREIRA DUTRA, NÚBIA BROETTO CUNHA, CARLOS ALBERTO GONÇALVES, CARMEM GOTTFRIED T - 45 IL-4 REGULATES THE LEVELS OF M3 MUSCARININC RECEPTORS IN RETINAL CELLS: THE ROLE OF IL-4 TYPE I RECEPTORS MARCELO GOMES GRANJA, LUIS EDUARDO GOMES BRAGA, ALINE ARAUJOS DOS SANTOS RABELO, ELIZABETH GIESTAL DE ARAUJO T - 46 PROLONGED MATERNAL SEPARATION AFFECTS HIPPOCAMPAL MORPHOLOGY AND NEUROTRANSMISSION ACCOMPANIED BY CHANGES IN NUTRITION-RELATED HORMONAL LEVELS PRISCILA BRISENO FROTA, DAVI DA CUNHA GONÇALVES, ANITA MAYARA FEITOSA SANTOS, CAMILA DE ALBUQUERQUE ALMEIDA, CELINA VIANA DE ARAÚJO, EMMANUEL GONÇALVES DE CASTRO ANDRADE, GEANNE M. DE ANDRADE, NUNO DE SOUSA, REINALDO BARRETO ORIÁ T - 47 THE PROTEIN CINASE C MODULATES THE CHOLINERGIC PHENOTYPE: POSSIBLE INVOLVEMENT OF INTERLEUKINS LUIS EDUARDO GOMES BRAGA, MARCELO GOMES GRANJA, ELIZABETH GIESTAL DE ARAUJO, ALINE ARAUJO DOS SANTOS T - 48 ENVIRONMENTAL ENRICHMENT REDUCES ANXIETY-LIKE BEHAVIOR ASSOCIATED WITH MASTICATORY DEPRIVATION IN ALBINO SWISS MICE RAISSA AIRES RIBEIRO BRINGEL, ANDRÉ PINHEIRO GURGEL FELÍCIO, FABÍOLA DE CARVALHO CHAVES DE SIQUEIRA MENDES, ALBERT LUIZ COSTA DA COSTA, ANA CARLA FADEL, RODRIGO PEREZ DA SILVA, DIEGO DE JESUS SILVA, MARCIA NUZANE AMORIM DE SOUZA, YANA MONTEZUMA SANTOS, CRISTOVAM WANDERLEY PICANÇO DINIZ, MARCIA CONSETINO KRONKA SOSTHENES T - 49 EVALUATION OF SOLVENTS AND CONTROLS FOR IN VITRO HUMAN CELL-BASED NEUROTOXICOLOGICAL STUDIES LETÍCIA APARECIDA BARBOSA HUMMEL, RÓBER BACHINSKI, ADRIANA BRANDÃO RIBEIRO LINHARES, JOSÉ MAURO GRANJEIRO, GUTEMBERG GOMES ALVES T - 50 INTERLEUKIN-2 (IL-2) AND RETINAL GANGLION CELL SURVIVAL: SIGNALING PATHWAYS INVOLVED LUCIENNE DE OLIVEIRA JESUS SOUZA, REGINA CÉLIA CUSSA KUBRUSLY, ELIZABETH GIESTAL DE ARAUJO T - 54 DIABETES DECREASES ASTROCYTIC GFAP EXPRESSION AFTER GLIOTOXIC LESION IN THE RAT BRAINSTEM EDUARDO FERNANDES BONDAN, MARIA DE FÁTIMA MONTEIRO MARTINS, FLÁVIO CESAR VIANI T - 55 DETERMINATION OF L-DOPA ADMINISTRATION EFFECTS IN A PARKINSON ANIMAL MODEL INDUCED BY 6-OHDA IGOR CARVALHO MARQUES, JÚNIA VIEIRA DOS SANTOS, MARCOS ROMÁRIO MATOS DE SOUZA, RAFAELA CARNEIRO CORDEIRO, LUCIANA DIAS BELCHIOR, ANDRÉ FÉRRER CARVALHO, DANIELLE SILVEIRA MACEDO T - 56 IN VITRO EFFECT OF AMPHETAMINE AND MINOCYCLINE IN THE MITOCHONDRIAL ACTIVITY OF RAT’S CEREBRAL CORTEX RAFAELA CARNEIRO CORDEIRO, IGOR CARVALHO MARQUES, CAMILA DANTAS MEDEIROS, JÚNIA VIEIRA DOS SANTOS, DANIELLE SILVEIRA MACEDO, ANDRÉ FÉRRER CARVALHO T - 57 MORPHOMETRIC EVALUATION AND IMMUNOFLUORESCENCE OF NEURONS OF THE PERIPHERAL NOCICEPTIVE SYSTEM IN OFFSPRING OF DIABETIC RATS TAÍS DE CAMPOS LIMA, CELINA MONTEIRO DA CRUZ LOTUFO T - 58 EFFECTS OF DEPRESSION ASSOCIATED OR NOT WITH ANTIDEPRESSANT TREATMENT ON THE RAT SALIVARY GLANDS PATRÍCIA HELENA ZANIER-GOMES, CARINA CARRARO PESSOA, TOMAZ EUGÊNIO DE ABREU SILVA, NANCI MENDES PINHEIRO, SIMONE DE SALES COSTA MOREIRA CARBONI, ADILHA MISSON RUA MICHELETTI, VIRGÍNIA OLIVEIRA CREMA T - 59 ASTROCYTES REGULATE GABAERGIC SYNAPSE FORMATION THROUGH THE TGF-BETA SIGNALING. LUAN PEREIRA DINIZ, VANESSA TORTELLI, LUCIANA FERREIRA ROMÃO, FLÁVIA CARVALHO ALCANTARA GOMES T - 60 IN VIVO EXPOSURE TO CAFFEINE CHANGES THE EXPRESSION OF A1 AND A2A ADENOSINE RECEPTORS IN THE CHICK EMBRYO RETINA: POSSIBLE EFFECTS ON THE GABAERGIC SYSTEM. RAFAEL BRITO DA SILVA, ROBERTO PAES DE CARVALHO, KARIN DA COSTA CALAZA T - 61 BEHAVIOR AND NEUROCHEMICAL ALTERATIONS RELATED TO CHRONIC ADMINISTRATION OF ETHANOL AND STRESS EXPOSURE DANIEL MOREIRA SILVA, GESSYNGER MORAIS SILVA, JULIANA FERNANDES SANTOS, MARCELO TADEU MARIN T - 62 NEUROGENIC NICHE OF ADULT BRAIN: ULTRASTRUCTURAL ASPECTS OF CELL ELEMENTS IN SUBVENTRICULAR ZONE, IN LONG-EVANS RATS CARLOS ALEXANDRE DOS SANTOS HAEMMERLE, SILVIA HONDA TAKADA, LEILA GUISSONI CAMPOS, MARIA INÊS NOGUEIRA, II-SEI WATANABE T - 63 INVESTIGATION OF SYNAPTIC DEFICITS IN SEPSIS: ROLE OF GLIAL CELLS CAROLINA ARAUJO MORAES, TÂNIA SPOHR, GABRIEL SANTOS, JOANA D’AVILLA, FERNANDO AUGUSTO BOZZA, FLÁVIA REGINA SOUZA LIMA, CLAUDIA BENJAMIM, FLÁVIA CARVALHO ALCANTARA GOMES T - 64 THE TROPHIC EFFECT OF IL-4 ON RETINAL GANGLION CELLS INVOLVES AN INCREASE IN BDNF EXPRESSION ALINE ARAUJO DOS SANTOS, ELIZABETH GIESTAL DE ARAUJO, ELIZABETH GIESTAL DE ARAUJO T - 65 STRESS IN PRE-PUBERTAL PERIOD AND CHRONIC EXPOSURE TO PALATABLE DIETS – EVALUATION OF PARAMETERS OF OXIDATIVE STRESS IN THE HIPPOCAMPUS OF ADULT MALE RATS. DANUSA MAR ARCEGO, CARINE LAMPERT, RACHEL KROLOW, CRISTIE NOSCHANG, TAMIRES BEN, ANA PAULA TONIAZZO, CARLA DALMAZ T - 66 EFFECT OF CHRONIC ADMINISTRATION OF TAMOXIFEN AND/OR ESTRADIOL ON OXIDATIVE PARAMETERS IN BRAIN OF OVARIECTOMIZED RATS CARINE LAMPERT, DANUSA MAR ARCEGO, RACHEL KROLOW, CRISTIE NOSCHANG, DANIELA PEREIRA LAUREANO, ISADORA FERREIRA LIMA, LUISA AMÁLIA DIEHL, CARLA DALMAZ, LETÍCIA FERREIRA PETTENUZZO, DEUSA VENDITE T - 67 NEUROPROTECTIVE EFFECT OF THE METABOTROPIC GLUTAMATE RECEPTOR 5 POSITIVE ALLOSTERIC MODULATORS IN HUNTINGTON´S DISEASE JULIANA GUIMARÃES DÓRIA, VANESSA COSTA DE MIRANDA DRUMMOND, FLAVIA RODRIGUES SILVA, TOMAS DOBRANSKY, FABIOLA MARA RIBEIRO T - 68 PHARMACOLOGICAL EFFECT OF BRAZILIAN SPIDER OMEGATOXINS IN CALCIUM SIGNALING OF TRIGEMINAL NOCICEPTIVE PATHWAY ELIZETE MARIA RITA PEREIRA, CÉLIO JOSÉ DE CASTRO JÚNIOR, ALESSANDRA HUBNER DE SOUZA, NANCY SCARDUA BINDA, LUCIENE BRUNO VIEIRA, RICARDO SANTIAGO GOMEZ, MARCUS VINICIUS GOMEZ T - 51 THE MECHANISMS OF STI1 (STRESS INDUCIBLE PROTEIN 1 ) SECRETION BY ASTROCYTES. MARCOS VINICIOS SALLES DIAS, GLAUCIA N. M. HAJJ, VILMA R. MARTINS T - 69 NEURONAL DIFFERENTIATION OF HUMAN NEUROBLASTOMA CELL LINE SH-SY5Y AND ITS USE FOR NEUROSCIENCE RESEARCH FERNANDA MARTINS LOPES, GIOVANA FERREIRA LONDERO, LIANDA MARENGO DE MEDEIROS, ROSALVA THEREZA MEURER, GADRIELA DELEVATI COLPO, MARILDA DA CRUZ FERNANDES, FLAVIO KAPCZINSKI, FÁBIO KLAMT T - 52 TURNOVER OF BETA-AMYLOID PRECURSOR PROTEIN IS REGULATED BY TWO MECHANISMS AT THE PLASMA MEMBRANE: THE UBIQUITIN-PROTEASOME PATHWAY AND INCORPORATION INTO T - 70 SYNAPTIC CONTACTS ON DENDRITIC SPINES OF THE POSTERODORSAL MEDIAL AMYGDALA OF RATS JORGE E. MOREIRA, ALBERTO A. RASIA-FILHO, RONALD PETRALIA, BECHARA KACHAR, JORGE E. MOREIRA 119 T - 71 LATERALIZATION OF INHIBITORY SYNAPTIC CONTACTS IN THE POSTERODORSAL MEDIAL AMYGDALA OF RATS JORGE E. MOREIRA, JANAINA BRUSCO, SUÉLEN MERLO, ALBERTO A. RASIA-FILHO, JORGE E. MOREIRA U-2 MORPHOLOGICAL ANALYSIS OF MIMOSA HOSTILIS BENTH SEEDS BY CYTOCHEMICAL METHODS. RENAN DA SILVA SANTOS, DEBORAH ALANI DE OLIVEIRA, MARIA IZABEL GALLÃO T - 72 THYROID HORMONES MEDIATE NEURON-ASTROCYTE INTERACTIONS: ROLE OF HEPARAN SULPHATE PROTEOGLYCANS ROMULO SPERDUTO DEZONNE, JOICE STIPURSKY, ANA PAULA BERGAMO ARAUJO, JADER NONES, MAURO SÉRGIO GONÇALVES PAVÃO, MARIMÉLIA PORCIONATTO, FLÁVIA CARVALHO ALCANTARA GOMES U-3 MORPHOLOGICAL ANALYSIS OF BAUHINIA FOFICATA, LIN SEEDS ARYELLI MAGALHÃES MACIEL, GABRIELA RODRIGUES FARIAS, GLEICYANNE VIEIRA DA COSTA, MARIA IZABEL GALLÃO T - 73 EFFECTS OF MATERNAL NICOTINE EXPOSURE DURING LACTATION ON HYPOTHALAMIC NEUROPEPTIDES EXPRESSION IN THE ADULT RAT CINTIA RODRIGUES PINHEIRO, VIVIANE YOUNES-RAPOZO, EGBERTO G. MOURA, ALEX C. MANHÃES, ANA PAULA SANTOS-SILVA, ELAINE DE OLIVEIRA, PATRICIA C. LISBOA T - 74 EVIDENCE OF PRION PROTEIN-STRESS INDUCIBLE PROTEIN 1 INTERACTION IN THE NEUROBIOLOGY OF TUMOR STEM CELLS REBECA PIATNICZKA IGLESIA, VILMA REGINA MARTINS, TIAGO GÓSS DOS SANTOS, MARILENE HOHMUTH LOPES T - 75 ENDOTHELIAL AND RADIAL GLIA CELLS INTERATION DURING CEREBRAL CORTEX DEVELOPMENT DANIEL FRANCIS FRANCO, JOICE STIPURSKY, FLÁVIA CARVALHO ALCANTARA GOMES T - 76 ENRICHED ENVIRONMENT INDUCES HIPPOCAMPUS CELLULAR PLASTICITY IN TYPE 1 DIABETIC RATS FRANCELE VALENTE PIAZZA, ETHIANE SEGABINAZI, LÍGIA ALINE CENTENARO, PATRÍCIA SEVERO DO NASCIMENTO, MATILDE ACHAVAL, SIMONE MARCUZZO T - 77 EFFECTS OF ENVIRONMENTAL ENRICHMENT ON MOTOR FUNCTIONS AND MUSCLE MORPHOLOGY ALTERATIONS IN A CEREBRAL PALSY RODENT MODEL MARÍLIA ROSSATO MARQUES, FELIPE DE SOUZA STIGGER, BRUNO SANTOS CAMPOS GOMES, ETHIANE SEGABINAZI, FRANCELE VALENTE PIAZZA, SÍLVIA BARBOSA, MATILDE ACHAVAL, SIMONE MARCUZZO T - 78 ENRICHED ENVIRONMENT PREVENTS MEMORY DEFICITS BUT NOT REVERT HIPPOCAMPUS CELLULAR SURVIVAL IN TYPE 1 DIABETIC RATS FRANCELE VALENTE PIAZZA, ETHIANE SEGABINAZI, LÍGIA ALINE CENTENARO, PATRÍCIA SEVERO DO NASCIMENTO, MATILDE ACHAVAL, SIMONE MARCUZZO T - 79 MATERNAL PROLACTIN INHIBITION DURING LATE-LACTATION IS ASSOCIATED WITH HIGHER NEUROPEPTIDE Y (NPY) IN ARCUATE NUCLEUS AND IN PARAVENTRICULAR NUCLEUS IN PROGENY AT ADULTHOOD VIVIANE RAPOZOYOUNES, NAYARA PEIXOTO-SILVA, LIGIA DE ALBUQUERQUE MAIA, ALEX C. MANHÃES, EGBERTO GASPAR DE MOURA, ELAINE DE OLIVEIRA, PATRICIA CRISTINA LISBOA T - 80 PI3K/AKT PATHWAY REGULATES MITOSIS OF NEURAL PROGENITORS DURING RETINAL DEVELOPMENT ISIS MORAES ORNELAS, THAYANE MARTINS SILVA, LUCIANNE FRAGEL MADEIRA, ANA LÚCIA MARQUES VENTURA T - 81 ACTIVATION OF P2X7 RECEPTORS INHIBITS THE PROLIFERATION OF LATE DEVELOPING RETINAL PROGENITORS IN CULTURE. THAYANE MARTINS SILVA, ISIS MORAES ORNELAS, ROXANA MAMANI ANCCASI, ANA LÚCIA MARQUES VENTURA U-4 OBSERVATION OF SEED LIPIDS FROM NORTHEASTERN SEMI ARID NATIVE SPECIES THROUGH THE NILE RED JOSÉ DE BRITO VIEIRA NETO, MARIA IZABEL GALLÃO, ASSUERO SILVA MEIRA, BRUNO MARQUES SOARES U-5 MORPHOLOGICA ANALYSIS OF CAESALPINIA PYRAMIDALIS TUL SEEDS PAMELA CLEMENTE DE MENESES SILVA, STELAMARIS DE OLIVEIRA PAULA, MARIA IZABEL GALLÃO U-6 LIGHT MICROSCOPY AS A TOOL TO INVESTIGATE PRETREATMENT EFFECT ON SUGARCANE RICARDO CHAVES VILELA, YURI ABUD, LILIAN T. COSTA, WANDERLEY DE SOUZA, CELSO SANT’ANNA U-7 EFFECTS OF ULTRAVIOLET RADIATION-B ON THE LEAF BLADE ORYZA SATIVA L. (POACEAE) CV EPAGRI 108 (1): CHANGES IN ULTRASTRUCTURAL ORGANIZATION. SÉRGIO LUIZ DE ALMEIDA, ÉDER CARLOS SCHMIDT, ANA CLAUDIA RODRIGUES, ZENILDA LAURITA BOUZON U-8 DETERMINATION OF THE MUTAGENESIS AND CYTOTOXIC ETHANOLIC EXTRACT OF PLANTAGO MAJOR (TANSAGEM) HELEN TAIS DA ROSA, TATIANE DA AQUINO, DINARA JAQUELINE MOURA U-9 MORPHOLOGICAL ANALYSIS OF ARTOCARPUS HETEROPHYLLUS LAM SEEDS GABRIELA ARAÚJO DE ABREU, ALINE PESSOA NEGREIROS, MARIA IZABEL GALLÃO U - 10 MORPHOLOGICAL CHANGES OF IN NATURA MANGOES SUBMITTED TO AN ELECTROLYZED WATER TREATMENT MARIA IZABEL GALLÃO, MARIA EDILEUZA LEITE, EBENEZER DE OLIVEIRA SILVA U - 11 MULTIPLICATION OF SOMATIC EMBRYOS OF BACTIS GASIPAES IN TEMPORARY IMMERSION SYSTEM (TIS) ANGELO SCHUABB HERINGER, DOUGLAS ANDRÉ STEINMACHER, MIGUEL PEDRO GUERRA U - 12 MATURATION AND CONVERSION OF SOMATIC EMBRYOS OF BACTRIS GASIPAES ANGELO SCHUABB HERINGER, DOUGLAS ANDRÉ STEINMACHER, MIGUEL PEDRO GUERRA U - 13 MORPHOLOGICAL AND HISTOCHEMICAL STUDY OF GREEN ALGAE (CLADOPHORA SP AND ULVA LACTUCA) TREATED WITH DIFFERENT SALINITIES LUZ KARIME POLO OSORIO, ÉDER C. SCHMIDT, CLAUDIANE GOUVEIA, MARTHIELLEN R. L. FELIX, FUNGYI CHOW, CRISTINA NASSAR, ZENILDA L. BOUZON U - 14 CHANGES IN THE MORPHOLOGY AND ULTRASTRUCTURE INDUCED BY ULTRAVIOLET RADIATION-B IN THE CARRAGENOPHYTE HYPNEA MUSCIFORMIS (RHODOPHYTA, GIGARTINALES) EDER CARLOS SCHMIDT, BEATRIZ PEREIRA, RODRIGO W. DOS SANTOS, CLAUDIANE GOUVEIA, FERNANDA RAMLOV, MARCELO MARASCHIN, ZENILDA L. BOUZON T - 82 ANALYSIS OF THE LIPID COMPOSITION OF THE ADULT MURINE BRAIN CEREBROSPINAL FLUID MARÍLIA KIMIE SHIMABUKURO, THAIS DE BARROS FERNANDES, GEÓRGIA CORREA ATELLA, CLAUDIA M.C. BATISTA, VALÉRIA DE MELLO-COELHO U - 15 MORPHOLOGICAL AND CELLULAR CHARACTERIZATION OF ZYGOTIC POLYEMBRYOS IN ARAUCARIA ANGUSTIFOLIA (BERT.) O. KUNTZE GLADYS DANIELA ROGGE RENNER, NEUSA STEINER, ÉDER CARLOS SCHMIDT, ZENILDA LAURITA BOUZON, FRANCINE LUNARDI FARIAS, MARIA LUIZA TOMAZI PEREIRA, MIGUEL PEDRO GUERRA T - 83 PRELIMINARY STUDIES OF CELL VIABILITY AND OXIDATIVE STRESS AFTER TREATMENT WITH NICOTINE OR VARENICLINE ON NEURONAL HIPPOCAMPAL PRIMARY CULTURES RAPHAEL TRINDADE DOS SANTOS, VIVIANE YOUNES RAPOZO, CLÁUDIO CARNEIRO FILGUEIRAS, YAEL ABREU VILLAÇA, ALEX CHRISTIAN MANHÃES U - 16 CELLULAR ALTERATIONS IN THE AGAROPHYTE GRACILARIA DOMINGENSIS TREATED WITH THE HEAVY METAL LEAD CLAUDIANE GOUVEIA, MARIANNE KREUSCH, EDER C. SCHMIDT, RODRIGO W. DOS SANTOS, ZENILDA L. BOUZON T - 84 ROLE OF TGF-Β1 ON RADIAL GLIA CELLS DIFFERENTIATION: REGULATION OF FOXG1 AND ERBB2 EXPRESSION. LAYS SOUZA DA SILVA, JOICE STIPURSKY SILVA, FLÁVIA CARVALHO ALCANTARA GOMES T85 HEPATOTOXIC (hCCP – MICROCYSTIN) AS WELL AS NON-HEPATOTOXIC CYANOPEPTIDES (n-hCCP) INFLUENCE NESTIN AND GFAP INTERMEDIATE FILAMENT ORGANIZATION IN ASTROCYTE ANJA BUBIK1,2, ROBERT FRANGEŽ3, TAMARA T LAH1 AND BOJAN SEDMAK1,2 U – Plant Cell Biology U1-U41 U-1 MORPHOLOGICAL CHARACTERIZATION OF MIMOSA CAESALPINIIFOLIA BENTH. SEED BY CYTOCHEMICAL METHODS RÔMULO MESQUITA FRANCO, LUCIANA DE VASCONCELOS REBOUÇAS, RENAN DA SILVA SANTOS, MARIA IZABEL GALLÃO U - 17 EFFECTS OF CADMIUM ON THE MORPHOLOGY AND CYTOCHEMISTRY OF THE RED MACROALGAE PTEROCLADIELLA CAPILLACEA MARTHIELLEN ROOSEVELT DE LIMA FELIX, ÉDER C. SCHMIDT, LUZ K. P. OSORIO, CLAUDIANE GOUVEIA, MARIANNE KREUSCH, RODRIGO DOS SANTOS, ZENILDA L. BOUZON U - 18 GLOBAL DNA METHYLATION LEVELS DURING ACCA SELLOWIANA (O. BERG) BURRET SOMATIC EMBRYOGENESIS BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY-MASS SPECTROMETRY HUGO PACHECO DE FREITAS FRAGA, LEILA DO NASCIMENTO VIEIRA, CLARISSA ALVES CAPRESTANO, DOUGLAS ANDRÉ STEINMACHER, GUSTAVO AMADEU MICKE, ROSETE PESCADOR, MIGUEL PEDRO GUERRA U - 19 ENDOGENOUS FREE POLYAMINES LEVELS OF ANANAS COMOSUS VAR. COMOSUS NODULE CLUSTER CULTURES USING TEMPORARY IMMERSION BIOREACTORS HUGO PACHECO DE FREITAS FRAGA, RAMON FELIPE SCHERER, ANTONIO CORRÊA GARCIA, LÍRIO LUIZ DAL VESCO, DOUGLAS ANDRÉ STEINMACHER, MIGUEL PEDRO GUERRA U - 20 HISTOCHEMICAL ANALYSIS OF CALLUSES OF CEDRELA FISSILIS VELOSO (MELIACEAE) FERNANDA KOKOWICZ PILATTI, EDER CARLOS SCHMIDT, ZENILDA LAURITA BOUZON, ANA MARIA VIANA U - 21 HISTOMORPHOLOGY DURING SOMATIC EMBRYOGENESIS INDUCTION OF ACCA SELLOWIANA (O. BERG). BURRET IN RESPONSE TO THE DNA 120 METHYLATION INHIBITOR 5-AZACITIDINE LEILA DO NASCIMENTO VIEIRA, HUGO PACHECO DE FREITAS FRAGA, ROSETE PESCADOR, MIGUEL PEDRO GUERRA U - 22 EFFECTS OF DNA METHYLATION INHIBITOR 5-AZACITIDINE ON THE CONVERSION OF ACCA SELLOWIANA SOMATIC EMBRYOS (O. BERG). BURRET LEILA DO NASCIMENTO VIEIRA, HUGO PACHECO DE FREITAS FRAGA, CLARISSA ALVES CAPRESTANO, ROSETE PESCADOR, MIGUEL PEDRO GUERRA U - 23 EFFECTS OF ULTRAVIOLET RADIATION-B IN THE RED ALGAE LAURENCIA CATARINENSIS (CERAMIALES, RHODOPHYTA DÉBORA TOMAZI PEREIRA, ÉDER CARLOS SCHMIDT, LUCIANE CRISTINA OURIQUES U - 24 COMPARATIVE ANALYSIS OF THE CELL ORGANIZATION OF PORPHYRA ACANTHOPHORA VAR. BRASILIENSIS UNDER THE EFFECTS OF ENVIRONMENTAL RADIATION, PAR, AND ARTIFICIAL ULTRAVIOLET RADIATION-B ZENILDA LAURITA BOUNZON, CARMEN S. ZITTA, RODRIGO W. DOS SANTOS, LUCIANE C. OURIQUES, CAROLINE DE FAVERI, CLAUDIANE GOUVEIA, EDER C. SCHMIDT U - 25 TISSUE-SPECIFIC EXPRESSION PATTERN ANALYSES OF OSASR5 (ABA, STRESS AND RIPENING) PROMOTER IN RICE (ORYZA SATIVA L.) MARIANA SCHUNEMANN, RAFAEL AUGUSTO ARENHART, ADRIANO SILVÉRIO, JORGE ERNESTO DE ARAUJO MARIATH, MÁRCIA MARIA AUXILIADORA NASCHENVENG PINHEIRO MARGIS U - 26 INCREASED SUPEROXIDE DISMUTASE ACTIVITY IN SOYBEAN TREATED WITH MANGANESE-DESFERRIOXAMINE B JÉSSICA BORDOTTI NOBRE ESPOSITO, BRENO PANNIA ESPOSITO, RICARDO ANTUNES AZEVEDO, SILVIA RIBEIRO DE SOUZA U - 27 EFFECTS OF COPPER ON THE ARCHITECTURE AND ULTRASTRUCTURE OF THE RED ALGAE GRACILARIA DOMINGENSIS (GRACILARIALES, RHODOPHYTA) MARIANNE G. KREUSCH, CLAUDIANE GOUVEIA, ÉDER C. SCHMIDT, RODRIGO W. DOS SANTOS, ZENILDA L. BOUZON U - 28 CYTOCHEMICAL AND ULTRASTRUCTURAL CHANGES INDUCED BY UVB RADIATION ON SEEDLING TETRASPOROPHYTIC OF PALISADA FLAGELIFERA (CERAMIALES, RHODOPHYTA). LUCIANE CRISTINA OURIQUES, DÉBORA TOMAZI PEREIRA, ÉDER CARLOS SCHMIDT U - 29 IMMUNOMODULATORY ACTIVITY OF AQUEOUS EXTRACT OBTAINED FROM THE STEM BARK OF BOWDICHIA VIRGILIOIDES KUNTH IN MICE LARISSA FERNANDA DE ARAUJO VIEIRA, MARIA DANIELMA DOS SANTOS REIS, ALTAIR ROGÉRIO ALVES BRANDÃO, THEREZINHA DE JESUS CALADO, EMILIANO BARRETO, SALETE SMANIOTTO U - 30 A PP2C PROTEIN IN RESPONSE TO COLD TEMPERATURE OF ARAUCARIA ANGUSTIFOLIA ROBERTA ALVARES CAMPOS, LEONARDO JO, FERNANDA PICCOLO PIERUZZI, JÉSSICA FERNANDES PEREIRA, IGOR LUCOVES SICCHI, NATALIA PISCIRILLO, SÂMILA BIANCHE LOPES, CAROLINE ARCANJO BUENO, AMANDA F MACEDO, ANDRÉ LUIS WENDT DOS SANTOS, ENY IOCHEVET SEGAL FLOH U - 31 ANATOMICAL CHANGES INDUCED BY ARSENIC IN WATER HYACINTH (EICHHORNIA CRASSIPES (MART.) SOLMS) IULLA NAIFF RABELO DE SOUZA REIS, JURACI ALVES DE OLIVEIRA, MARÍLIA CONTIN VENTRELLA, JOSÉ CAMBRAIA, REGIANE APARECIDA CANATTO U - 32 PROTEOMIC ANALYSIS OF TWO VARIETIES OF ZEA MAYS INOCULATED WITH AZOSPIRILLUM BRASILENSE FP2 ALEXANDRO CÉZAR FALEIRO, ELIANDRO ESPINDULA, TOMAS PELLIZZARO PEREIRA, ANA CAROLINA MAISONNAVE ARISI U - 33 MORPHO-HYSTOLOGICAL FEATURES IN IN VITRO PROTOCORMLIKE BODIES OF CATTLEYA TIGRINA RAFAELA DUARTE DE LIZ, MARISA SANTOS, YOHAN FRITSCHE, ROSETE PESCADOR, MIGUEL PEDRO GUERRA MARIA LUIZA TOMAZI PEREIRA, BRUNA SCHEID, NEUSA STEINER, GLADYS DANIELA ROGGE RENNER, ÉDER CARLOS SCHMIDT, ZENILDA BOUZON, MIGUEL PEDRO GUERRA U - 40 MORPHOLOGICAL CHARACTERIZATION BY SCANNING ELECTRON MICROSCOPY OF SOMATIC PRO-EMBRYOS OF ARAUCARIA ANGUSTIFOLIA FRANCINE LUNARDI FARIAS SOARES, MARIA LUIZA TOMAZI PEREIRA, BRUNA SCHEID, NEUSA STEINER, GLADYS DANIELA ROGGE RENNER, ÉDER CARLOS SCHMIDT, ZENILDA BOUZON, MIGUEL PEDRO GUERRA U - 41 ROLE OF NITRIC OXIDE MOLECULE IN TOLERANCE TO ARSENIC IN PISTIA STRATIOTES: SIGNAL OR ANTIOXIDANT? FERNANDA DOS SANTOS FARNESE, JURACI ALVES DE OLIVEIRA, LUHAN ISAAC SIMAN, REGIANE APARECIDA CANATTO, CRISTIANE JOVELINA DA SILVA V – Plasma Membrane and Organelles V1-V10 V-1 AQUAPORIN LOCALIZATION IN ANT EXCRETORY SYSTEM MARIA DO CARMO QUEIROZ FIALHO, DIHEGO DE OLIVEIRA AZEVEDO, LUIZA CARLA BARBOSA MARTINS, JOSÉ EDUARDO SERRÃO V-2 WATER-FLUXES AND THE FUNCTION OF CANALICULI IN THE MIDGUT OF PHIBALOSOMA PHYLLINUM (PHASMIDA, PHASMATIDAE) EMILIANO CARNEIRO MONTEIRO, WALTER RIBEIRO TERRA, ALBERTO AUGUSTO GONÇALVES DE FREITAS CASTRO RIBEIRO V-3 THE DIGESTIVE SYSTEM OF BUCEPHALOGONIA XANTHOPHIS (HEMIPTERA, CICADELLIDAE): THE ORGANIZATION OF THE LUMINAL SYSTEM OF MEMBRANES ALEXANDRE HIROSHI UTIYAMA, WALTER RIBEIRO TERRA, ALBERTO FREITAS RIBEIRO V-4 INFLUENCE OF ALKALINE PH ON MAGNETOSOME FORMATION BY “CANDIDATUS MAGNETOVIBRIO BLAKEMOREI” PEDRO ERNESTO LOPES LEÃO, FERNANDA ABREU, DENNIS A. BAZYLINSKI, ULYSSES LINS V-5 PATHWAYS REGULATING SECRETORY LYSOSOME BIOGENESIS AND SECRETION ABBIE L NEILSON, ERICA B WILSON, JOSEPHINE L MEADE, JACQUELYN BOND, GRAHAM P COOK V-6 EXPOSURE OF LUMINAL MEMBRANES OF LLC-PK1 CELLS TO ANG II INDUCES DIMERIZATION OF AT1/AT2 RECEPTORS TO ACTIVATE SERCA AND TO PROMOTE CA2+ MOBILIZATION FERNANDA MAGALHÃES FERRÃO, LUCIENNE SILVA LARA, FLÁVIA AXELBAND, JULIANA DIAS, ADRIANA K CARMONA, ROSANA I REIS, CLÁUDIO M COSTA-NETO, ADALBERTO VIEYRA, JENNIFER LOWE V-7 STRUCTURAL CHARACTERIZATION OF CARGO-BINDING SITES OF THE MU4-SUBUNIT OF ADAPTOR PROTEIN COMPLEX 4 BREYAN H. ROSS, YIMO LIN, PATRICIA V. BURGOS, JUAN S. BONIFACINO, GONZALO A. MARDONES V-8 ORGANIZATION OF THE DIGESTIVE SYSTEM OF THE FRUIT FLY ANASTREPHA SERPENTINA (DIPTERA: TEPHRITIDAE) CAMILA SILVA LEAL, ALEXANDRE HIROSHI UTIYAMA, ALBERTO FREITAS RIBEIRO V-9 RAPID ASSEMBLY AND INTERNALIZATION OF CAVEOLAE PROMOTE RESEALING IN INJURED CELLS AND MUSCLE FIBERS PATRICIA ELAINE DE ALMEIDA, MATTHIAS CORROTTE, CHRISTINA TAM, MARIA CECILIA FERNANDES, MAURO CORTEZ, TIMOTHY K. MAUGEL, NORMA W. ANDREWS U - 34 NITRIC OXIDE ATTENUATE THE OXIDATIVE STRESS AS-INDUCED IN LETTUCE LEAVES NEIDIQUELE MARIA SILVEIRA, JURACI ALVES DE OLIVEIRA, LUHAN ISAAC SIMAN, FERNANDA SANTOS FARNESE, CLÉBERSON RIBEIRO, REGIANE APARECIDA CANATTO V - 10 THE LISOSOMAL TARGETING OF CD4 BY HIV-1 NEF REQUIRES Γ2, A NOVEL ISOFORM OF GAMMA ADAPTIN EULÁLIA MARIA LIMA DA SILVA, RODRIGO ORLANDINI DE CASTRO, LUIS LAMBERTI PINTO DA SILVA U - 35 EFFECTS OF DIFFERENT CYTOKININS ON CELL PROLIFERATION OF ROLLINIA MUCOSA (JACQ.) BAILL. FOR SECONDARY METABOLITES PRODUCTION THIAGO JOSÉ DE SOUZA BARBOZA, CECÍLIA DE AZEVEDO SOUZA, DÉBORA DE AGUIAR LAGE, NORMA ALBARELLO X – Proteolysis U - 36 CYTOTOXICITY STUDY OF NORANTEA BRASILIENSIS METHANOL EXTRACTS USING BRINE SHRIMP LETHALITY TEST ANNA FLÁVIA RODRIGUES MORTANI VILARDO, GRAZIELA DA SILVA MELLO, ANALU FONSECA DE SÁ, NORMA ALBARELLO U - 37 EFFECTS OF ULTRAVIOLET-B RADIATION IN CELL ORGANIZATION DURING THE INITIAL DEVELOPMENT OF NEMALION HELMINHOIDES (VELLEY IN WITH.) BATTERS (NEMALIALES, RHODOPHYTA) ELIANA DE MEDEIROS OLIVEIRA, LUCIANE CRISTINA OURIQUES U - 38 ROLE OF THE GOLGI APPARATUS IN STORAGE OF SECONDARY METABOLITES IN RED ALGA LAURENCIA DENDROIDEA. LILIAN JORGE HILL, LEONARDO TAVARES SALGADO U - 39 HYSTOCHEMICAL CHARACTERIZATION OF SOMATIC PROEMBRYOS OF ARAUCARIA ANGUSTIFOLIA FRANCINE LUNARDI FARIAS SOARES, X1-X10 X-1 AGH IS A NEW HEMOGLOBIN ALPHA-CHAIN FRAGMENT WITH ANTINOCICEPTIVE BIOLOGICAL ACTIVITY NATALIA MAZINI RIBEIRO, LILIAN C. RUSSO, LEANDRO M. CASTRO, CAMILA S. DALE, ALANA R. FIGUEIREDO, FABIO C. GOZZO, VANESSA RIOLI, EMER S. FERRO X -2 PURIFICATION AND PARTIAL BIOCHEMICAL CHARACTERIZATION OF A METALLOPROTEASE FROM BOTHROPS MOOJENI VENOM THALITA KRISTHINA ALVES SILVA, CARLA CRISTINE NEVES MAMEDE, MAYARA RIBEIRO DE QUEIROZ, BRUNA BARBOSA DE SOUSA, ANA LUIZA ZACOUR MARINHO, NADIA CRISTINA GOMES DE MORAIS, KELLY CORTES FONSECA, DÉBORAH FERNANDA DA CUNHA PEREIRA, THAÍS MIRANDA MIGLIORINI, MARIANA SANTOS MATIAS, FÁBIO DE OLIVEIRA 121 X -3 INTRACELLULAR PEPTIDE ANALYSES IN CELLS EXPRESSING THE IMMUNE PROTEASOME: POSSIBLE CORRELATIONS TO CELL SIGNALING ELISABETE RODRIGUES DO MONTE SILVA, EMER SUAVINHO FERRO, VANESSA RIOLI, LILIAN C RUSSO, LEANDRO M. DE CASTRO, FÁBIO C. GOZZO X -4 IDENTIFICATION OF E3 UBIQUITIN- LIGASE SCF1(FBXO25) SUBSTRATES THROUGH THE UBIQUITINATION IN VITRO ASSAY USING PROTEIN MICROARRAY FELIPE ROBERTI TEIXEIRA, ADRIANA OLIVEIRA MANFIOLLI, CLÁUDIA SOSSAI SOARES, ANA CAROLIAN HUMANES, MARCELO DAMARIO GOMES X -5 FUNCTIONAL CHARACTERIZATION OF THE FBXO25 NUCLEAR BODIES (FANDS) ADRIANA OLIVEIRA MANFIOLLI, FELIPE ROBERTI TEIXEIRA, MUNIRA MUHAMMAD ABDEL BAQUI, CLÁUDIA SOSSAI SOARES, ANA CAROLINA HUMANES, CACILDA DIAS PEREIRA, MARCELO DAMÁRIO GOMES X -6 PURIFICATION AND CHARACTERIZATION BIOCHEMISTRY OF P3G2: AN ENZYME COAGULANT AND Α- FIBRINOGENOLYTIC SNAKE VENOM OF BOTHROPS MOOJENI MARIANA SANTOS MATIAS, ANA LUIZA ZACOUR MARINHO, MAYARA RIBEIRO DE QUEIROZ, CARLA CRISTINE NEVES MAMEDE, DÉBORAH FERNANDA DA CUNHA PEREIRA, THALITA KRISTHINA ALVES SILVA, KELLY CORTES FONSECA, THAÍS MIRANDA MIGLIORINI, BRUNA BARBOSA DE SOUSA, NADIA CRISTINA GOMES DE MORAIS, IGOR DE AZAMBUJA QUEIROZ RIBEIRO, FÁBIO DE OLIVEIRA X -7 CLONING AND EXPRESSION OF A RECOMBINANT SERINE PROTEASE INHIBITOR FROM LOXOSCELES INTERMEDIA VENOM: A MEMBER OF SERPIN FAMILY LUIZA HELENA GREMSKI, JENIFER NOWATZKI, DILZA TREVISAN SILVA, RAFAEL BERTONI DA SILVEIRA, WALDEMIRO GREMSKI, ANDREA SENFF RIBEIRO, OLGA MEIRI CHAIM, SILVIO SANCHES VEIGA X -8 ANALYSIS OF SUBSTRATES AND PRODUCTS OF NEUROLYSIN (EC 3.4.24.16) IN MOUSE BRAIN USING QUANTITATIVE PEPTIDOMICS LEANDRO MANTOVANI DE CASTRO, VITOR OLIVEIRA, FÁBIO CÉZAR GOZZO, EMER SUAVINHO FERRO X -9 EVALUATION OF BIOLOGICAL ACTIVITY OF MATRIX METALLOPROTEINASES FROM THE VENOM OF BOTHROPS ATROX AFTER AUTOPROTEOLYSIS REBECCA TAVARES E SILVA, MARIA DAS DORES NOGUEIRA NORONHA, ANDRÉ MIASATO HIGA, ANDRÉ LUIZ FERREIRA DA SILVA, KARLA NUNES DA SILVA, PAULA CRISTINA BRÍGIDO CARVALHO NEVES, JORGE LUIS LÓPEZLOZANO X -10 EDEMATOGENIC ACTIVITY INDUCED BY TOXINS FROM THE BOTHROPS ATROX VENOM AFTER AUTOPROTEOLYSIS REBECCA TAVARES E SILVA, MARIA DAS DORES NOGUEIRA NORONHA, ANDRÉ MIASATO HIGA, ANDRÉ LUIZ FERREIRA DA SILVA, KARLA NUNES DA SILVA, PAULA CRISTINA BRÍGIDO CARVALHO NEVES, JORGE LUIS LÓPEZ-LOZANO Z – Stem Cells Z1-Z42 Z-1 EFFECTS OF THE PLATELET–ACTIVATING FACTOR ON THE PLURIPOTENCY OF MURINE EMBRYONIC STEM CELLS PAULA VIEGAS PEREIRA SIGNORETTI, LUCIANNE FRAGEL MADEIRA Z-2 ULTRASTRUCTURE OF THE REGENERATIVE CELLS OF THE MIDGUT OF CERAEOCHRYSA CLAVERI (NAVÁS, 1911) (NEUROPTERA: CHRYSOPIDAE) ELTON LUIZ SCUDELER, DANIELA CARVALHO DOS SANTOS, MONIQUE CAMPOS PEREIRA, ANA SILVIA GIMENES GARCIA, PATRÍCIA FERNANDA FELIPE PINHEIRO Z-3 ISOLATION AND CHARACTERIZATION OF STEM/PROGENITOR CELLS PROPERTIES OBTAINED OF HUMAN BREAST CANCER CELL LINE MILENE PEREIRA MOREIRA, GEOVANNI DANTAS CASSALI, LUCIANA MARIA SILVA Z-4 LEPTIN FRAGMENTS MODULATE THE MURINE HEMATOPIESIS CAROLINA CARVALHO DIAS, AMANDA NOGUEIRA-PEDRO, CHRISTIANO M. VAZ BARBOSA, VANI XAVIER DE OLIVEIRA JUNIOR, ANTONIO MIRANDA, EDGAR J. PAREDES-GAMERO Z-5 GLUTATHIONE DEPENDENT OSTEOGENIC DIFFERENTIATION OF SKIN MESENCHYMAL PROGENITORS OCCURS THROUGH MAPK SIGNALING MARIA FERNANDA FORNI, LAURA POLIZEL, ADRIANO SARTORI, ERIK HALCSIK, ETELVINO BECHARA, MARI CLEIDE SOGAYAR Z-6 VIABILITY, PROLIFERATION AND GENOTOXIC EFFECTS OF ELECTROSPRAYING ON MESENCHYMAL STEM CELLS DAIKELLY IGLESIAS BRAGHIROLLI, FERNANDA ZAMBONNI, PEDRO CHAGASTELLES, DINARA MOURA, JENIFER SAFFI, JOÃO ANTÔNIO PEGAS HENRIQUES, DIOGO ANDRÉ PILGER, PATRICIA PRANKE Z-7 TISSUE REGENERATION, BIOENGINEERING AND STEM CELLS: BIBLIOMETRIC ANALYSIS OF PUBLICATIONS BETWEEN 2000 AND 2010 CRISTIANE REGINA SCHER, PEDRO CHAGASTELLES, ALEXANDRE MENEGHELLO FUENTEFRIA, PATRICIA PRANKE Z-8 ISOLATION AND NEUROGENIC DIFFERENTIATION OF MESENCHYMAL STEM CELLS FROM HUMAN DECIDUOS TEETH PULP VIRGINIA ETGES HELFER, THAYANE CRESTANI, KERLIN QUINTILIANO, DIOGO ANDRÉ PILGER, PATRÍCIA PRANKE Z-9 INFLUENCE OF THE TIME OF INCUBATION ON MESENCHYMAL STEM CELL ADHESION ON NANOFIBER MATRICES DAVI SILVEIRA DOS SANTOSS, KERLIN QUINTILIANO, THAYANE CRESTANI, VIRGINIA ETGES HELFER, DAIKELLY IGLESIAS BRAGHIROLLI, DIOGO ANDRÉ PILGER, PATRICIA PRANKE Z - 10 INCORPORATION OF VEGF ON PLGA SCAFFOLDS PRODUCED BY ELECTROSPINNING ANNELISE RIBEIRO DA ROSA, KERLIN QUINTILIANO, DANIELA STEFFENS, NÍVEO STEFFEN, DIOGO ANDRÉ PILGER, PATRICIA PRANKE Z - 11 DEVELOPMENT OF NGF LOADED SCAFFOLDS AND ASSOCIATION WITH MESENCHYMAL STEM CELLS FOR NERVE TISSUE ENGINEERING KERLIN QUINTILIANO, THAYANE ANTONIOLLI CRESTANI, DAVI SILVEIRA DOS SANTOS, VIRGINIA ETGES HELFER, ANNELISE RIBEIRO DA ROSA, GERALDO PEREIRA JOTZ, DIOGO ANDRÉ PILGER, PATRICIA PRANKE Z - 12 A NEW BIOMATERIAL OF NANOFIBERS WITH THE MICROALGA SPIRULINA AS SCAFFOLDS FOR USE IN TISSUE ENGINEERING DANIELA STEFFENS, MICHELLE LERSCH, ANNELISE ROSA, CRISTIANE SCHER, THAYANE CRESTANI, MICHELE GREQUE MORAIS, JORGE ALBERTO VIEIRA DA COSTA, PATRICIA PRANKE Z - 13 EVALUATION OF BONE REGENERATION PROMOTED BY THE ASSOCIATION OF SCAFFOLDS SEEDED WITH STEM CELLS FROM THE PULP OF HUMAN DECIDUOUS TEETH GERSON ARISOLY XAVIER ACASIGUA, LISIANE BERNARDI, DAIKELLY IGLESIAS BRAGHIROLLI, MANOEL SANT”ANA FILHO, PATRICIA PRANKE, ANNA CHRISTINA MEDEIROS FOSSATI Z - 14 MESENCHYMAL STEM CELL ADHESION AND PROLIFERATION RATES ON SCAFFOLDS OF POLY(LACTIC-CO-GLYCOLIC ACID) (PLGA) WITH DIFFERENT NANOFIBER DIAMETERS FERNANDA ZAMBONI, MARIANA DE CONTO FIN, DAIKELLY IGLESIAS BRAGHIROLLI, DIOGO ANDRÉ PILGER, PATRICIA PRANKE Z - 15 CHARACTERIZATION OF CULTURE EXPANDED MULTIPOTENT MESENCHYMAL STROMAL CELLS FROM EQUINE ADIPOSE TISSUE ARMANDO DE MATTOS CARVALHO, ANA LUCIA MILUZZI YAMADA, MARJORIE ASSIS GOLIM, LUIS EMILIANO C ÁLVAREZ, LUCIANA LEAL JORGE, MARIANA LOPES, ELENICE DEFFUNE, CARLOS ALBERTO HUSSNI, ANA LIZ GARCIA ALVES Z - 16 ISOLATION OF MESENCHYMAL STEM CELLS FROM THE CARDIAC MUSCLE OF GALLUS GALLUS RAQUEL CALLONI, PATRICK TURCK, GABRIHEL STUMPF VIEGAS, ELVIRA ALÍCIA APARÍCIO CORDERO, DIEGO BONATTO Z - 17 THE ROLE OF CASRS DURING ADULT RAT MESENCHYMAL STEM CELLS PROLIFERATION AND APOPTOSIS FERNANDA MARIA POLICARPO TONELLI, RODRIGO RIBEIRO RESENDE, LUIZ ORLANDO LADEIRA Z - 18 BIOMODULATION OF HUMAN EMBRYONIC STEM CELLS AFTER LOW POWER LASER IRRADIATION JULIANA F. MANGOLIN, ANDREZA C. DE SIQUEIRA SILVA, ERIANE ELLER DE SIQUEIRA, SUEMI SOARES BATISTA, CRISTINA PACHECO SOARES, NEWTON SOARES DA SILVA Z - 19 MESENCHYMAL STEM CELLS THERAPY IN ANIMAL MODEL OF DILATED CARDIOMYOPATHY INDUCED BY DOXORUBICIN: TROPONIN I LEVELS AND HISTOLOGICAL EVALUATION. HELENA FLORES MELLO, PRISCILLA DOMINGUES MÖRSCHBÄCHER, TUANE NERISSA ALVES GARCEZ, ANA HELENA DA ROSA PAZ, ALESSANDRA BILESKI MAGRISSO, VIVIAM NUNES PIGNONE, LANUCHA FIDELIS DA LUZ MOURA, ANELISE BONILLA TRINDADE, ELIZABETH OBINO CIRNELIMA, EMERSON ANTÔNIO CONTESINI Z - 20 EVALUATION OF FREQUENCY AND FUNCTIONALITY OF MESENCHYMAL CELL POPULATIONS IN OBESE AND EX-OBESE HUMAN SUBCUTANEOUS ADIPOSE TISSUE KARINA RIBEIRO DA SILVA, JOAO REGIS IVAR CARNEIRO, CESAR CLAUDIO-DA-SILVA, ANTÔNIO AUGUSTO PEIXOTO DE SOUZA, RADOVAN BOROJEVIC, LEANDRA SANTOS BAPTISTA Z - 21 HUMAN MENSTRUAL BLOOD DERIVED MESENCHYMAL CELLS AS NEW HUMAN FEEDER-LAYER SYSTEM FOR HUMAN EMBRYONIC STEM CELLS DANÚBIA SILVA DOS SANTOS, VANESSA CARVALHO COELHO DE OLIVEIRA, KARINA DUTRA ASENSI, LEANDRO VAIRO, ADRIANA BASTOS CARVALHO, ANTONIO CARLOS CAMPOS DE CARVALHO, REGINA COELI DOS SANTOS GOLDENBERG Z - 22 MAGNETIC RESONANCE IMAGING DETECTS EMBRYONIC STEM CELLS LABELED WITH SUPERPARAMAGNETIC IRON OXIDE NANOPARTICLES IN THE MURINE HEART GUILHERME VISCONDE BRASIL, DANÚBIA SILVA DOS SANTOS, ANDRÉIA DE VASCONCELOS DOS SANTOS, CLERIO FRANCISCO DE AZEVEDO FILHO, FERNANDA FREIRE TOVAR MOLL, ROSÁLIA MENDEZ OTERO, REGINA COELI DOS SANTOS GOLDENBERG, ANTONIO CARLOS CAMPOS DE CARVALHO Z - 23 DEVELOPMENT OF A NEW METHODOLOGY OF HUMAN DERMIS STEM CELL ENCAPSULATION IN CARRAGEENAN HYDROGEL ADDELI BEZ BATTI ANGULSKI, MICHELE RODE, TALITA JEREMIAS, LEILA HAYASHI, ANDREA GONÇALVES TRENTIN, GIORDANO WOSGRAU CALLONI Z - 24 17-BETA-ESTRADIOL EFFECTS IN MESENCHYMAL STEM CELLS UNDER OSTEOGENIC DIFFERENTIATION IN VITRO. PATRICK TURCK, RAQUEL CALLONI, GABRIHEL STUMPF VIEGAS, ELVIRA ALÍCIA APARÍCIO CORDERO, DIEGO BONATTO Z - 25 EFFECTS OF DEXAMETHASONE ON MESENCHYMAL STEM CELLS MORPHOLOGY, FUNCTIONALITY AND VIABILITY NATÁLIA SCHNEIDER, FABIANY DA COSTA GONÇALVES, HELENA FLORES MELLO, CRISTINA FLORES, ELIZABETH 122 OBINO CIRNE LIMA, EDUARDO PANDOLFI PASSOS, LUÍSE MEURER, ANA HELENA DA ROSA PAZ Z - 26 MESENCHYMAL STEM CELLS FROM MENSTRUAL BLOOD ARE MORE RESISTANT TO OXIDATIVE STRESS THAN PLURIPOTENT STEM CELLS KARINA DUTRA ASENSI, RODRIGO SOARES FORTUNATO, DANIELLE FERREIRA DE REZENDE, THAÍSA SILVA PACHECO, DANÚBIA SILVA DOS SANTOS, DEIVID DE CARVALHO RODRIGUES, TAIS HANAE KASAI-BRUNSWICK, ELAINE CRISTINA LIMA DE SOUZA, ANTONIO CARLOS CAMPOS DE CARVALHO, DENISE PIRES CARVALHO, ADRIANA BASTOS CARVALHO, REGINA COELI DOS SANTOS GOLDENBERG Z - 41 THREE-DIMENSIONAL SUGARCANE-BASED SCAFFOLD FOR HUMAN MESENCHYMAL STEM CELLS CULTURE MARYANA ROBERTA PEDROSA DIAS, BELYSSA SANTOS DE MORAES, ANDRIU DOS SANTOS CATENA, LUANA ALBUQUERQUE BARROS, SILVÂNIA TAVARES PAZ, ELIETE CAVALCANTI DA SILVA, JOSÉ LAMARTINE DE ANDRADE AGUIAR, PALOMA LYS DE MEDEIROS Z - 42 A FLORAL REPRESSOR BROTHER OF FT AND TFL1 (BFT) MODULATES FLOWERING INITIATION UNDER HIGH SALINITY IN ARABIDOPSIS JE CHANG WOO, JAE YONG RYU, PIL JOON SEO, CHUNG-MO PARK Z - 27 COMPARISON BETWEEN FIBROBLASTS AND MESENCHYMAL STEM CELLS DERIVED FROM DERMAL AND ADIPOSE TISSUE CARLA ABDO BROHEM, CAROLINE LEAL RADOSKI, CAMILA MIRANDA DE CARVALHO, MARCELA CONTADOR BAPTISTA, BRUNA BASTOS SWINKA, FLÁVIA CRYSTINA SANTI, CÍCERO DE ANDRADE URBAN, RUTH MARIA GRAF, ISRAEL HENRIQUE STOKFISZ FEFERMAN, MÁRCIO LORENCINI Z - 28 IN VITRO AND IN VIVO OSTEOINDUCTIVE POTENTIAL OF POLY-3HYDROXYBUTYRATE/POLYBUTYLENE SUCCINATE SCAFFOLDS COLONIZED BY ADIPOSE TISSUE DERIVED STEM CELLS THAIS MARIA DA MATA MARTINS, ANA CLÁUDIA CHAGAS DE PAULA, ALESSANDRA ZONARI, ALEXANDRA RODRIGUES PEREIRA DA SILVA, SILVIENE NOVIKOFF, ALFREDO MIRANDA DE GOES Z - 29 CHARACTERIZATION OF MULTIPOTENT MESENCHYMAL STROMAL CELLS OBTAINED FROM WHOLE BONE MARROW PLATED WITHOUT FICOLL GRADIENT NAYARA DE FREITAS MARTINS, FERNANDA BARRA FRANCO, FERNANDA DE SOUZA MARTINS, PATRÍCIA FIDELIS DE OLIVEIRA Z - 30 HAIR FOLLICLE DERIVED MESENCHYMAL CELLS SUPPORT UNDIFFERENTIATED GROWTH OF HUMAN EMBRYONIC STEM CELLS VANESSA CARVALHO COELHO DE OLIVEIRA, DANÚBIA SILVA DOS SANTOS, LEANDRO VAIRO, ADRIANA BASTOS CARVALHO, ANTÔNIO CARLOS CAMPOS DE CARVALHO, REGINA COELI DOS SANTOS GOLDENBERG Z - 31 CELL THERAPY AND PHYSICAL ACTIVITY: A THERAPEUTIC APPROACH IN A MICE SPINAL CORD INJURY TAMIRES BRAGA MASSOTO, MARINA BAIRROS HEBERLE, FERNANDA MARTINS DE ALMEIDA, ADRIANO BIANCALANA, ANA MARIA BLANCO MARTINEZ, SUELEN ADRIANI MARQUES Z - 32 EFFECTS OF AGING ON THE SUBVENTRICULAR ZONE OF THE MURINE BRAIN ARE REGULATED BY GROWTH HORMONE TREATMENT IN VIVO AND IN VITRO. MARILIA KIMIE SHIMABUKURO, LARISSA GUTMAN PARANHOS LANGHI, CHIN JIA LIN, ROGER CHAMMAS, CLAUDIA MARIA DE CASTRO BATISTA, VALÉRIA DE MELLO-COELHO Z - 33 EXTRACELLULAR ADENINE NUCLEOTIDES METABOLISM IN MESENCHYMAL STEM CELLS FROM DIFFERENT MURINE TISSUES ISABELE CRISTIANA ISER, PAULA ANDREGHETTO BRACCO, RAFAEL FERNANDES ZANIN, NANCE BEYER NARDI, ANA MARIA OLIVEIRA BATTASTINI, MÁRCIA ROSÂNGELA WINK Z - 34 ROLE OF CARDIAC MICROENVIRONMENT ON CARDIOMYOGENIC DIFFERENTIATION OF STEM CELLS ANNY WALOSKI ROBERT, ANA PAULA RESSETTI ABUD, ANDRESSA VAZ SCHITTINI, ALEJANDRO CORREA, MARISE B. A. COSTA, FRANCISCO D. A. COSTA, ALEXANDRA SENEGAGLIA, PAULO S. BROFMAN, MARCO AUGUSTO STIMAMIGLIO Z - 35 FUNCIONAL AND PHENOTYPICAL CHARACTERIZATION OF ACUTE CHAGASIC CARDIOMYOPATHY IN CHIMERIC MICE CAMILA IANSEN IRION, BRUNO DIAS PAREDES, GUILHERME VISCONDE BRASIL, SANDRO TORRENTES DA CUNHA, DÉBORA BASTOS MELLO, ISALIRA PEROBA REZENDE RAMOS, ANTONIO CARLOS CAMPOS DE CARVALHO, ADRIANA BASTOS CARVALHO, REGINA COELI DOS SANTOS GOLDENBERG Z - 36 ALTERED OXYGEN METABOLISM ASSOCIATED TO NEUROGENESIS OF INDUCED PLURIPOTENT STEM CELLS DERIVED FROM A SCHIZOPHRENIC PATIENT BRUNA DA SILVEIRA PAULSEN, RENATA DE MORAES MACIEL, ANTONIO GALINA, MARIANA SOUZA SILVEIRA, CLEIDE DOS SANTOS SOUZA, HANNAH DRUMMOND, ERNESTO NASCIMENTO POZZATTO, HAMILTON SILVA JUNIOR, LEONARDO CHICAYBAM, RAFFAEL MASSUDA, PEDRO SETTI PERDIGÃO, MARTIN BONAMINO, PAULO SILVA BELMONTE DE ABREU, NEWTON GOLÇALVES CASTRO, HELENA BRENTANI, STEVENS KASTRUP REHEN Z - 37 NEW APPROACH TO CULTURE HUMAN ADIPOSE STEM CELL SEEDED ON PHB-HV SCAFFOLDS FOR BONE TISSUE ENGINEERING APPLICATION ANA CLAUDIA CHAGAS DE PAULA, ALEXANDRA RODRIGUES PEREIRA DA SILVA, ALESSANDRA ZONARI, THAÍS MARIA DA MATA MARTINS, SILVIENE NOVIKOFF, ALFREDO MIRANDA GOES Z - 38 CHARACTERIZATION OF ISOLATED AND CULTURE EXPANDED SYNOVIAL MESENCHYMAL STEM CELLS FROM HORSES SYNOVIUM. ANA LIZ GARCIA ALVES, JAYESH DUDHIA, ROBERTA FERRO DE GODOY, ROGER K W SMITH Z - 39 EPIDERMAL GROWTH FACTOR-SCAFFOLDS AND MESENCHYMAL STEM CELLS: A NEW APPROACH FOR NERVE TISSUE ENGINEERING THAYANE CRESTANI, KERLIN QUINTILIANO, VIRGINIA ETGES HELFER, DAVI SILVEIRA DOS SANTOS, GERALDO JOTZ, DIOGO ANDRÉ PILGER, PATRICIA PRANKE Z - 40 ANALYSIS OF THE UNFOLDED PROTEIN RESPONSE (UPR) IN EMBRYONIC STEM CELLS DAIANNE NEVES MANDARINO TORRES, MARIANA PARANHOS STELLING, STEVENS KASTRUP REHEN, LUCIANA BARRETO CHIARINI 123 Author Index ABDELHAY ESFW ABRAHAO TB ABRANCHES MV ABREU GA ABREU IS ABREU JG ABREU LA ABREU RMM ABRUNHOSA VM ACASIGUA GAX ADACHI P AFONSO RCH AGOSTINHO LA AGOSTINI LP AGUIAR DP AGUIAR JMC AGUIAR JUNIOR O AGUIAR RB AGUIAR TT AIRES MB ALBARELLO N ALBUQUERQUE AV ALEVI KCC ALLODI A ALLODI S ALMEIDA ACF ALMEIDA CEV ALMEIDA CJG ALMEIDA GS ALMEIDA JC ALMEIDA MES ALMEIDA MF ALMEIDA NK ALMEIDA PE ALMEIDA RSC ALMEIDA SL ALMEIDA TF ALMEIDA VR ALONSO GC ALPONTI RF ALVES ALG ALVES GG ALVES HHO ALVES LL ALVES LP ALVES MGCF ALVES NR ALVES RMS AMARAL MGC AMARAL RF AMARAL SS AMBRÓSIO F AMMAR D AMORIM R ANDRADE HM ANDRADE IR ANDRADE JKF ANDRADE LM ANDRADE LO ANGULSKI ABB ANHÊ ACB ANSELMO TC ANTONIOLI E APOLINARIO LM AQUINO T ARAGAO AB ARAGÃO BC ARAN V ARAÚJO AF ARAUJO CB ARAÚJO DAM ARAUJO EG ARAÚJO EP ARAÚJO FA ARAUJO H ARAÚJO HMM ARAÚJO HSS ARAUJO JUNIOR ALC ARAÚJO JÚNIOR RF ARAUJO KCL ARAÚJO LCC ARAÚJO MDD ARAÚJO RMS ARAÚJO TG ARAÚJO WM ARAÚJO-MARTINS L ARCEGO DM ARCHANGELO LF B - 117 J - 37 B -48 U-9 H - 16 B – 221, J – 46, N – 42, N - 43 J - 28 S-3 A - 81 Z - 13 P - 32 B - 194 T - 10 B - 93 N - 33 N - 51 D - 94 B - 250 A - 83 D – 40, D - 42 U – 35, U - 36 D - 11 D – 18, D – 113, D – 117, D – 118, D – 124 T-4 F – 3, I - 1 R -43 B-3 C - 108 C - 14 N - 32 I-9 T - 35 R -69 V-9 J - 34 U-7 C-5 K - 10 H - 19 A - 119 Z – 15, Z - 38 E -6, E -9, S – 22, T - 49 A - 84 C - 70 I-7 A-8 D - 115 L-1 A - 76 B - 97 G-5 C – 76, C – 78 N - 34 R -48 J - 31 M -1 B - 180 B-1 R -9, R -11 Z - 23 S-9 C - 43 A-4 C - 55 E -4 J - 40 T - 25 J - 12 C - 92 G - 12 B – 60, B – 206, B – 208, F – 10, T – 45, T -50, T - 64 C – 115, J - 48 C – 68, C - 110 J - 45 N – 19, N - 48 B – 98, B – 99, B - 157 B -207 B – 94, B - 111 R -32 C - 111 B - 195 O -4 B -209, M -9 B - 76 T - 64 T - 65 A - 26 ARISI ACM ARMELIN HA ARMILIATO N ARO AA ARRUDA LB ARRUDA RF ASENSI KD ASSIS LHC ASSUNÇÃO CLS ASSUNÇÃO FS ATHAYDE RM ATTIAS M ATTIAS M AUGUSTO LMM AUGUSTO LS AUGUSTO TM AVELAR GF AVILA RA AYRES R AYUB LC AZEREDO-OLIVEIRA MTV AZEVEDO CS AZEVEDO EPC AZEVEDO OGR BACK SH BAHIA D BALARINI MK BANZATO TP BÁO SN BAPTISTA LS BAPTISTA MS BARBOSA AS BARBOSA CRR BARBOSA FAR BARBOSA GO BARBOSA JLP BARBOSA LA BARBOZA TJS BARCELOS LS BARJA-FIDALGO C BARRETO EO BARROS CM BARROS LG BARROS LRC BARROS NMT BARROS SBM BARROS TAA BARROS ZAV BASTIANI MA BASTOS IMF BASTOS NF BATISTA AA BATISTA JR M BATISTA JÚNIOR ML BATISTA NV BATISTA TH BATTATASTINI AMO BECKENKAMP A BEGNINI KR BEGUELINI MR BEHLING CS BELETTI ME BELIZÁRIO J BELLINI MH BELTRAN JSO BELUSSO JV BENCHIMOL M BENFATO MS BENJAMIM CF BERALDI EJ BERBERT LR BERNARDES PTT BERNARDO PS BERNHARD T BERNI MA BERTAGLIA RS BERTOZZI E BETTEGA M BEVILACQUA E BIANCALANA A BIANCARDI MF U - 32 B – 82, B – 112, F – 1, J - 25 D - 61 P - 15 G - 18 B - 158 Z - 26 D - 68 B - 189 B - 106 C - 23 R -27, R -29, R 35 S - 24 H - 17 A - 102 B - 137 D - 140 R -75 F - 15 B - 186 D – 16, D – 18, D – 19, D – 20, D – 21, D – 22, D 86 S - 28 C - 75 C – 49, R -17 C - 22 J – 34, R -36 D - 31 D - 50 B – 12, B – 15, B - 22 S – 15, Z - 20 G – 21, S - 18 K - 15 B-7 A - 16 P - 35 C - 41 B - 44 U - 35 K - 15 B – 171, C – 112, C - 117 C - 92 A – 12, A – 74, A – 78, H – 16, T 27 C - 21 C - 83 B - 235 G - 30 I-3 Q -3 C - 16 R -67, S - 28 B - 239 A – 64, B - 226 C - 67 F - 26 C - 35 F - 20 B - 181 B – 108, B - 153 B - 225 D - 90 D - 57 D – 92, D – 121, D – 122, D – 123, D – 125, D – 126, R -32 B - 184 Q -3 G - 17 C – 84, C - 87 G – 6, M -1, R -3, R -4, R -7, R - 8 C – 31 C – 69, C – 70, C – 85, I - 8 T - 43 I - 13 C – 24, C - 25 B - 236 E -4 N - 48 A - 31 R -7 G - 37 D – 63, D – 120, D - 139 S - 30 D - 133 BIASOLI D BIGUETTI CC BINDA NETO I BIRUKOV K BIRUKOVA A BISPO-DA-SILVA LB BITTENCOURT JÚNIOR PIH BIZARRO HDAS BLASIOS JUNIOR V BLOISE FFB BOMFIM AMD BOMFIM CCB BOMFIM RS BONATTO D BONDAN EF BONFIM DC BORBA HR BORDIN DL BORELLA MI BORELLI P BORGES AC BORGES BN BORGES HL BORGES LF BOTTAS LS BOURCKHARDT GF BOUZON ZL BOZZA FA BOZZA P BOZZA PT BRACCO PA BRACHER F BRAGA CA BRAGA LC BRAGA LEG BRAGHIROLLI DI BRANCO PC BRANDT JZ BRASIL GV BRENO MC BRIE I-C BRINGEL RAR BRITO IRR BRITO MV BRITO NETO JM BRITO NM BRITO TLA BROHEM CA BRUNO AN BRUSCO J BUBIK A BUENO GCL BUFFOLO MA BUFFON A BURGOS PV BUSATTO FF BUSTAMANTE H BUTTOW NC BUZATO CBC CABRAL FILHO PE CAETANO FH CAGNON VHA CAIXEIRO APA CAIXETA DC CALAZA KC CALDEIRA EJ CALDINI EG CALLONI GW CALLONI R CÂMARA AR CAMARGO KC CAMARGOS DS CAMPOS D CAMPOS JUNIOR PHA CAMPOS LM CAMPOS MRC CAMPOS MS CAMPOS RA CAMPOS SGP CAMPOS SPC CAMPOS TA CAMPOS VMA CANATTO RA CANDIDO NM CANIUGUIR A B - 52 C-8 J - 36 J-2 J-1 A - 33 C - 27 C - 107 M -10 H-4 T - 28 B - 60 A - 85 Z – 16, Z - 24 T – 53, T - 54 J - 30 B – 2, R -1 B - 156 A - 98 G - 17 Q -5 B – 195, B – 200, O -16, O -17 B – 52, B – 143, C - 93 P – 27, P - 29 T - 29 N - 34 U – 7, U – 13, U – 14, U – 16, U – 17, U – 24, U 27 G - 38 R -68 C – 95, C – 97, C – 107, C – 108, R -61, R -62 B - 244 R -60 D - 114 O -3 T - 47 Z-6 I – 5, S - 26 D - 17 Z - 22 J - 10 B - 58 T - 48 B - 227 R -73 N – 1, N - 50 B - 171 P - 12 Z - 27 B - 147 T - 70 T-85 B - 65 N - 26 B – 87, B – 95, B – 108, B – 153, B - 170 T - 52 B - 152 T - 52 T - 43 J - 11 S - 19 G – 3, L - 1 D – 91, D – 96, D – 98, D – 105, D - 129 A - 18 A - 65 T - 60 C-1 P - 11 A – 16, Z - 23 Z - 16 N - 45 N - 37 B - 42 T - 12 D - 72 N - 40 A - 49 D - 137 U - 30 D - 51 R -64 A - 66 B – 3, B - 14 U – 31, U - 34 B - 36 A - 46 124 CANO MIN CANUTO KS CAPITANIO JS CAPPELLARI AR CAPUCHO C CARA DC CARBALLO CB CARDEAL LBS CARDIN LT CARDOSO C CARDOSO LHD CARDOSO MG CARDOSO SV CARDOSO VM CARMO ER CARMO LAS CARNEIRO K CARREIRA ACO CARVALHO ACC CARVALHO ADZ CARVALHO AF CARVALHO AL CARVALHO ALH CARVALHO AM CARVALHO CR CARVALHO DFF CARVALHO HF CARVALHO JG CARVALHO LA CARVALHO MGC CARVALHO ND CARVALHO RVH CARVALHO SC CASALI VVC CASANELLO P CASTELUCCI BG CASTRO LM CASTRO NFC CASTRUCCI AML CATAE AF CATISTI R CATROXO MHB CAVALCANTE LA CAVALCANTI DP CAVALHEIRO GRC CAVALHEIRO RP CECON E CELLA N CERRI PS CHAIM OM CHAMMAS R CHAVES CR CHAVES NL CHAVES RS CHEDID RA CHEN Y-H CHIARINI LB CHIELA ECF CHIN-YUAN H CHUANG Y-L CHUNG HT CICCONE CC CIPRIANO I CISTERNA AEC CLOUTHIER DE COELHO VM COGO AJD COLANERI GN COLLARES T COLLARES-BUZATO CB COLQUHOUN A CONTESINI EA COOK GP CORDEIRO E CORDEIRO IR CORDEIRO RC CORDEIRO RS CORREA A CORREA CL CORRÊA DEC CORRÊA JR CORREA OMT CORRÊA-FEITOSA VL CORRÊA-JUNIOR JD CORREA-NORONHA SAA CORTE ALVA CÔRTE-REAL S CORTEZ BA COSSOLIN JFS COSTA AFAF COSTA AGB A – 3, A – 35, F – 18, F - 21 B - 20 B – 71, B - 212 B - 181 D - 14 C – 19, C – 34, C – 35, C - 39 A – 85, C - 91 B - 61 C - 30 B - 249 J - 22 O -11 B - 11 N - 30 B - 252 A - 89 N - 13 K - 14 Z - 22 C - 57 T - 56 B - 217 B - 150 Z - 15 C – 40, C – 43, C - 44 T - 75 A – 36, A – 105, B – 137, B – 138, E -11, H – 12, I – 6, P - 35 B-6 T - 19 B - 154 G - 36 R -53 C - 60 L-2 A - 46 D – 15, D - 131 X -8 D - 107 A – 120, J - 41 S-1 D-1 S-7 T - 19 S - 14 N - 35 B - 86 C - 105 J – 15, J - 27 G - 32 S - 25 B - 166 F-4 B -15 T - 42 N - 47 B-5 B – 237, F – 19, Z - 40 S-2 G – 1, G - 2 G-1 C - 22 P–2 N - 10 P-4 N-2 C - 77 H - 18 B - 78 B - 213 F-8 B – 177, B - 178 Z - 19 V-5 C - 17 N-4 T - 56 N - 21 O -13 I-1 H - 10 B - 172 D - 13 A – 21, A - 22 A – 107, F – 6, R -10, S - 5 B - 77 F - 11 A – 97, A - 100 B - 130 G-8 B - 211 C - 66 COSTA ALC COSTA BRC COSTA CFP COSTA CGCM COSTA EBO COSTA FLP COSTA GA COSTA GMJ COSTA JP COSTA LJ COSTA MFD COSTA ML COSTA MSA COSTA NCS COSTA RA COSTA RMB COSTA SM COUTO BHCV CRECZYNSKI-PASA TB CREMA VO CRESTANI T CRUZ CKF CRUZ CU CRUZ MVT CRUZ TA CUNHA MS CURY GCG CUSTÓDIO MR DALMAZ C DAL-PAI-SILVA M DALPIAN F DAMASCENO EM DAMATTA RA DAMIANI RM DANTAS VWM DAVID LRS DEALMEIDA CE DELELLA FK DEZONNE RS DIAMANTE MAS DIAS BRS DIAS CC DIAS FCR DIAS G DIAS GS DIAS MA DIAS MHS DIAS MRP DIAS MVS DIAS OFG DIAS RB DIAS RS DIAS SMG DIAS WB DIAZ BL DÍAZ JAM DINIZ CWP DINIZ JR JAP DINIZ LP DOBRANSKY T DOLDER H DOLDER MAH DOMENICONI RF DONADIO JL DONATTI L DORE CMPG DÓRIA JG DREWES CC DUARTE MEL DUARTE ML DUBOC LF DUMAS ML DUPIN E DURANTE AC DURVALE MC EL-CHEIKH MC ELLWANGER JH EMRICH LC ESPINDOLA FS ESPOSITO JBN ESPREAFICO EM ESQUISATTO MAM ESTRELA MS EVANGELISTA RC I-2 D - 24 B - 182 H-6 T - 14 B - 131 D - 74 A - 114 R -44, R -48, R 50, R -58 R -13 H – 5, N - 40 A - 112 B - 129 C - 40 B - 201 B – 183, S - 20 R -9 A – 72, B - 189 H – 7, T - 58 Z - 39 D - 116 A – 38, K - 11 T - 38 R -29 R -58 R -47 A - 96 D – 107, D – 108, D – 113, D – 117, D – 118, D - 124D – 127, D - 130 T – 30, T - 65 A – 31, A - 37 T-8 A – 56, A - 57 A – 83, C – 99, E -7, E -8, R -49, R -57 B - 204 B - 146 B - 12 B – 14, B - 17 D-3 T - 72 A – 91, B - 197 R -71 Z-4 D - 54 D – 43, D – 64 K-8 B - 132 B - 112 Z - 41 T - 51 B - 25 H - 14 C - 29 B - 57 B - 45 B - 215 B - 68 T – 11, T - 31 R -73 T - 59 T – 23, T - 32 D – 95, G - 31 A – 60, A – 91, B – 197, D – 28, D – 29, D - 30 D - 67 B - 45 A – 67, A - 92 B - 67 T - 67 B - 165 H - 17 A - 25 A - 56 B - 55 N - 51 B - 98 M -6 F-9 T - 12 A - 74 A – 58, A – 62, A - 65 U - 26 B – 228, B – 241, B -243, B – 247, B – 248, B – 249, Q -5 P – 1, P – 2, P – 6, P – 8, P – 9, P – 13 B - 240 C - 51 FAÇANHA ALO FAÇANHA AR FACCIOLI CK FACCIOLI LAP FACINA CH FADEL AC FAGANELLO J FALCAO VTFL FALEIRO AC FARIA AMC FARIA JAQA FARIA NETO HCC FARIA PA FARIA PR FARIA TF FARIAS ND FARIAS PS FARNESE FS FARO TAS FARSKY SHP FAVARO PMB FÉ AR FEIO DCA FEITOZA F FELBER YT FELICIONI F FELIPPE DC FELISBINO SL FELIX RL FÊO HB FERNANDES DC FERNANDES KPS FERNANDES LR FERNANDES PCC FERRÃO FM FERRÃO PM FERRARI MFR FERREIRA AF FERREIRA AMR FERREIRA AT FERREIRA AVM FERREIRA FF FERREIRA FRL FERREIRA GJ FERREIRA KBO FERREIRA LC FERREIRA LRL FERREIRA PCG FERREIRA WAS FERREIRA-MACHADO SC FERRER VP FERRO ES FIALHO MCQ FIERRO IM FIGLIUOLO VR FIGUEIRA SF FIGUEIREDO CC FIGUEIREDO DTA FIGUEIREDO JB FIGUEIREDO MS FILIPPIN FB FIORE APZP FISCHER-FODOR E FLÁVIO KAPCZINSKI F FLOH EIS FLORIM JC FOCHI RA FOCK RA FOGAÇA E FOGUEL D FONSECA AS FONSECA BF FONSECA CG FONSECA FL FONSECA HLS FONSECA MC FONSECA RC FONSECA WF FONTANETTI CS FONTES A FONTES AM FORNI MF FORTES GB FORTI FL FOSSATI ACM FRADE PCR FRAGA HPF FRANÇA FS FRANÇA LR H - 18 B – 63, B – 131, B - 158 N – 44, N - 49 K-1 D – 53, D – 86 T - 26 R -54 J - 11 U - 32 C - 118 O -12 C - 74 G - 16 B – 18, B - 107 C - 42 B - 62 D - 42 J – 32, J – 42, U 42 B - 200 B - 165 B - 122 C - 86 B - 238 B - 178 D - 81 D – 87, D – 101 N - 31 B – 26, B – 216, D-3 U - 17 P - 13 J – 16, S - 21 C – 21, C – 58, C - 62 B - 185 R -33 V-6 P - 26 A – 29, T – 13, T – 29, T – 35, T – 42 R -43 B – 163, B - 164 H - 15 R -26 N - 39 B -127 T-4 C - 96 P - 14 T - 37 O -18, U - 37 O -16 B -17 P-5 G – 12, X -1, X -8 V-1 C - 86 J - 47 B - 219 B -127 R -67 C - 85 J - 43 B - 214 F - 14 B - 58 G - 34 U - 30 I - 10 D - 100 C - 11 D - 78 C – 66, C - 75 B -16, B – 20, B – 31, F - 5 J - 46 B – 88,T – 18, T – 2, T - 25 R -28 H - 11 T-3 C - 39 D - 106 A - 64 S – 16, S - 19 H-6 Z–5 B - 162 M -4, M -5 Z - 13 A - 111 U – 18, U - 19 B - 29 D – 36, D – 68, D – 70, D – 72, D – 73, D – 74, D 125 FRANÇA MM FRANCELIN C FRANCISCO G FRANCISCO JS FRANCO DG FRANCO FO FRANCO RM FRANCO-BELUSSI L FRANK S FRANKE SIR FREIRE NETO CA FREITAS C FREITAS EHS FREITAS JR FREITAS KM FREITAS LJA FREITAS MAR FREITAS SM FREITAS VM FRIESLAND A FROTA PB FRUET AC FUNCHAL C FUNGARO TP FURLAN AS FURTADO IMA FURTADO RR GALHARDO MS GALINDO LT GALLÃO MI GAMA P GAMEIRO J GANDOLPHI LC GARCIA ASG GARCIA E COSTA F GARCIA HC GARCIA MCD GARCIA PMA GARDESANI WKM GARNIQUE ADMB GARZONI LR GASTARDELO TS GATTASS CR GAUTHIER-ROUVIÈRE C GEISSLER K GELALETI GB GENTI-RAIMONDI S GERALDO AHPS GHIZONI H GIANNOTTI KC GICQUEL T GIL CD GIORDANO RJ GIROL AP GITIRANA LB GOBBO MG GODA M GODOY BB GOES AM GÓES RM GOLDENBER RCS GOMES A GOMES AL GOMES FCA GOMES FILHO SA GOMES FS GOMES GF GOMES JMM GOMES JR GOMES L GOMES LF GOMES MD GOMES MPSM GOMES PF GOMEZ MV GOMEZ RS GONÇALVES BF GONÇALVES CA GONÇALVES GJM GONÇALVES IB GONÇALVES RC GONÇALVES WA GONTIJO JAR GONZAGA ACR GONZÁLEZ A GONZÁLEZ MJ – 80, D – 82, D – 140 B - 104 A-5 B - 166 A - 108 C - 100 F - 26 U-1 C-2 N - 24 A – 17, A – 32, T - 40 B - 72 R -68 O -10 B – 151, C - 10 A – 60, G - 31 D – 44, D - 46 R -74 B - 75 B – 73, B – 115, B – 168, B - 199 M -2 T - 46 G - 30 L-4 C - 32 A - 45 N - 16 R -72 P - 11 I-4 U – 1, U – 10, U – 2, U – 5, U - 9 A – 1, F – 14, F – 17, H – 1, J - 39 R -53 D - 108 D - 77 D - 93 D - 11 H-8 D - 82 S - 18 B -192 A – 39, P - 26 B - 41 B – 37, B - 38 J-6 C - 36 B - 103 A - 55 P - 32 A-1 C - 59 A - 79 C - 72 B – 61, B - 80 C-3 A – 108, K - 6 A - 63 M -13 J-8 Z – 28, Z - 37 A – 63, D – 24, D – 41, D – 69 K – 1, K – 8, P – 34, Z – 21, Z – 26, Z – 30, Z - 35 T - 75 N – 28, N - 35 T – 34, T – 59, T – 63, T – 72, T 84 C - 110 C - 115 T - 11 F-6 B – 151, B – 186, B – 252, C - 10, N - 37 P-7 D - 66 X -4, X - 5 T - 18 A - 15 T – 14, T - 68 T-1 D - 23 C - 73 B - 107 A - 26 J - 16 C - 79 N - 14 D - 32 B - 109 A - 117 GORJÃO R GÓRNIAK SL GORTZ LW GOSMANN G GOTTFRIED C GOULART FILHO LR GOULART LR GOUVEIA C GOZZO EC GRANATO AEC GRANJA MG GRANJEIRO JM GREGORIO LS GREMSKI LH GRUND LZ GUATIMOSIM C GUEDES CES GUEDES HG GUEIROS FILHO FJ GUERRA CR GUERRA MP GUERRA MT GUERRANT RL GUIDO BC GUILHERME RF GUILLAUME E GUIMARÃES IM GUIMARÃES LPTPG GUSMAN GS HA KS HACKENHAAR FS HADDAD NF HAEMMERLE CAS HAGA RB HAGE AAP HAIFIG I HAJJ G HAMAO K HAN SW HANSEN HP HATANAKA E HAUSEN MA HAYASHI JPM HAYASHI MAF HEBLING A HELFER VE HELUANY CS HENRIQUE BVM HENRIQUES F HENRIQUES JAP HENRIQUES MG HENRIQUES MGMO HERINGER AS HERLINGER AL HERNANDES L HETZL AC HILL LJ HIRAIWA SH HIRAKI KRN HIRATA CL HOFMANN JUNIOR AE HOLLMANN G HOSCH NG HOSOYA H HOTTZ ED HROMAS R HUMMEL LAB IAMONTE M IERARDI DF IGLESIA RP IKEDA ET INTROÍNI GO IONTA M IONTA M IRION CI ISER IC JACKSON K JACOB CRO JAEGER MC JAEGER RG JAMUR MC JASIULIONIS MG JASIULIONIS MG JESUS LWO JIMÉNEZ-GARCÍA LF JOANITTI GA A - 84 S - 23 G - 35 B -160 T - 44 B – 174, M -9 B -209 U - 16 X -3 A - 59 T - 45 G – 11, G – 15, S - 15 D-4 X -7 J-9 T – 3, T - 38 R -63 B - 94 M -6, M -10 R -30 U – 11, U – 12, U – 15, U – 18, U – 19, U – 21, U – 22, U – 33, U – 40, U - 41 D - 55 R -17 B - 172 C - 69 J-6 T - 32 B - 38 J - 42 B - 53 C - 31 B -66 T - 62 J - 20 A - 116 A - 19 T - 36 A - 73 K – 3, Q -1, Q -4 B - 119 A – 44, C - 47 K-7 C - 58 B – 224, B - 251 P-7 Z-8 J-7 B - 242 C - 67 B – 156, B – 173, C – 4, F - 11 C - 109 R -52 U – 11, U - 12 B – 85, B - 232 R -22, R -23 D - 91 U - 39 D - 104 B - 175 J - 27 A – 68, D - 103 F-3 F - 22 A – 73, A – 75, M -7 G - 38 B - 56 T - 49 A - 48 B - 49 T - 74 T - 71 D - 52 F - 20 F – 22, F – 27, H - 10 Z - 35 Z - 33 S - 13 L-7 B - 196 B – 74, B – 125, B – 167, B - 220 A – 28, A – 49, R -25 B – 30, B – 49, B - 78 J – 19, O -8, O 9, O -15 A - 98 A - 118 B - 75 JOAZEIRO PP JONATHAN GS JORGE EC JUSTO GZ KAJISHIMA AL KALDIS P KANG KR KANNO TYN KATO KC KATZ SG KAWAHARA R KAWALL HG KEDE J KEMPINAS WG KERKIS I KIDO LA KIM S-M KIOKA N KIPPER FC KISAKI MK KLAMT F KOBARG J KOBUS K KONDO T KOS L KRAUSE LMF KREBSBACH P KREUSCH MG KRUEGER B KUHNE F LABRIOLA L LACERDA JZ LACERDA SMSN LACERDA TCS LACOUTH P LADEIRA LO LADISLAU T LAGENTE V LAH TT LAMERS ML LAMIM T LAMPERT C LANCELLOTTI M LANDIM BC LANGHI LGP LARA NLM LARANJO LT LAUAND C LAURA SIMON L LAURINDO FRM LAUS AC LAZARI MFM LAZARINI M LAZAROTTI MP LE DIAGON MMRQ LE DOUARIN N LEAL CS LEAL L LEÃO PEL LEDUR PF LEE CS LEIGUEZ E LEIMGRUBER C LEITÃO A LEITÃO RFC LEITE EL LEITE GB LEITE JCA LEITE MF LEITE RP LEME AFP LEMOS MS LENZ G LEÓDIDO ACM LEONARDO AMC LEPLETIER A LEYTON BJK LIMA ABA LIMA ACC LIMA AM LIMA C LIMA EM LIMA FRS LIMA GB LIMA GDA LIMA LPO LIMA MA LIMA MS LIMA NS D – 12, D – 15, D – 131, P - 17 R -40 N - 15 B – 148, B - 190 N - 42 F - 16 J - 13 N - 29 R -10 D - 88 B - 176 A - 13 J - 14 D – 49, D – 50, D - 55 B – 251, K - 5 D - 98 A - 20 B - 96 A - 69 G - 27 B – 29, B – 149, H – 13, T - 69 A – 45, J – 5, J – 8, J - 21 N - 46 A - 75 N - 36 B - 179 A - 67 U - 27 C - 36 A - 105 G-7 B - 83 D - 73 B - 222 A - 96 Z - 17 B - 198 A – 79, A - 80 Q -2 B – 25, B – 110, E -10 N - 11 T - 66 R -20, R -42, R 46, R -47 M -8 C - 77 D - 70 A - 50 B - 113 K – 12, K - 13 A – 71, I – 7, J – 24, J – 37, S - 21 B - 217 D – 109, J - 29 J-4 B - 33 J - 10 N - 33 V-8 B - 245 V-4 B - 40 J - 13 C - 104 C-6 B - 56 B - 133 A – 8, B - 67 D-2 N-7 B – 1, F - 4 D – 28, D - 30 B – 176, J - 40 D - 92 B – 23, B – 40, B – 89, B – 218, S 2 N - 17 A – 19, A - 50 I - 12 O -14 E -8 D - 21 I – 12, R -56 J-9 O -1 O -2 O -4 B – 88, B – 90, B - 97 R -62 D - 34 R -24 B - 115 B - 173 A - 52 126 LIMA PDL LIMA PHS LIMA TC LIMA TDFA LIMA THA LIMA VM LIMA WS LIMA-SALGADO TM LINHARES ABR LINO-NETO J LINS MP LINS U LISBOA PC LISONI FCR LIZ RD LOBATO S LOBBA ARM LOGULLO C LOMBELLO CB LONGHI MT LOPES AA LOPES AG LOPES DV LOPES FM LOPES JR LOPES KAR LOPES MH LOPES TS LÓPEZ-LOZANO JL LORENCINI M LORENCINI RM LORENZI VCB LORENZON AR LOTFI CFP LOTUFO CMC LOURENÇO ES LOURO ID LOWE J LU Q LU Z LUCAS JZ LUCENA SV LUNA MS MABUCHI I MACCHI BM MACEDO DS MACHADO ACL MACHADO CF MACHADO CM MACHADO DE MACHADO JR J MACHADO SM MACHADO-SANTELLI GM MADEIRA LF MADEKUROZWA MADI-RAVAZZI L MAGRINI TD MAI C MAIA RC MALASPINA O MALDONADO CA MALDONADO IRSC MALPARTIDA HMG MALTA JCO MANCINI K MANCINI KC MANFIOLLI AO MANGOLIN JF MANHÃES AC MANSANO ESB MANSANO RAW MARANGON CG MARÇAL LN MARCELINO RC MARCIANO RS MARCONDES PG MARCUZZO S MARDONES GA MARGIS MMANP MARIA-ENGLER SS MARIA-ENGLER SS MARIANO LIF MARIN MT MARKUS RP MARQUES CAB MARQUES IC MARQUES MB MARQUES MF MARQUES MJ MARQUES MR MARQUES PE B - 238 B - 191 B – 121, T - 57 F - 23 R -54 B – 2, R -1 R -19 C - 32 H-9 A – 18, D – 39, D – 43, D – 64, D – 66 A - 30 V-4 A – 52, T – 73, T - 79 C - 30 U - 33 B - 54 B - 70 A - 99 E -3 J - 15 C - 101 B - 132 C - 54 T - 69 B - 100 A-7 B – 43, T - 74 G - 15 X -10, X - 9 Z - 27 D - 129 A - 23 D - 120 B - 104 T - 57 S - 22 B – 92, B - 93 J – 22, V - 6 M -2 B-5 R -65 J - 33 B – 81, J - 3 M -13 C - 99 T - 55 A - 90 T - 33 D - 67 C - 61 B - 219 G - 10 A – 10, B – 113, B – 130, N - 18 T – 9, Z - 1 M MC -3 A - 95 A-2 A - 17 B – 140, B - 236 L – 6, L – 7, S - 1 B – 21, C - 6 R -15, R -16 B - 245 A - 121 A - 51 A - 57 X -5 Z - 18 T - 83 R -22, R -23 G-7 B - 135 B – 51, S - 12 D - 106 B - 31 B - 50 T – 76, T – 77, T - 78 V-7 U - 25 P – 30, J – 20, P - 19 B - 214 D - 126 T - 61 C – 100, C – 105, C - 113 N - 11 T - 55 B - 123 A - 94 A – 47, C – 55, C - 60 T - 77 C - 15 MARQUES SA MARQUES-SANTOS LF MARQUES-SANTOS LFM MARRIEL NB MARTIN PKM MARTINATTI CK MARTINELLI PM MARTINEZ AMB MARTINEZ PAM MARTINHO OCL MARTINS AAB MARTINS AB MARTINS ACA MARTINS AF MARTINS AMC MARTINS CM MARTINS CS MARTINS DFC MARTINS FA MARTINS FF MARTINS GF MARTINS GVF MARTINS L MARTINS MFM MARTINS MR MARTINS NF MARTINS PR MARTINS PR MARTINS RAP MARTINS TMM MARTINS TVF MARTINS VR MARTINS VR MARTINS WK MASCHIO DA MASSOTO TB MATIAS ICP MATIAS MS MATOS AV MATOS DG MATOS NS MATSUBARA FH MATSUMOTO MA MATSUMURA CY MATTA SLP MATTE U MATTOS RM MAYA-MONTEIRO CM MAYORAL EE MAZUCATOC VM MAZZI DPSL MEDEIROS J-VR MEDEIROS PL MEDINA BNSP MEISSNER GO MELISO FM MELLO AA MELLO CLO MELLO CP MELLO HF MELLO JC MELLO PA MELLO-COELHO V MELO AC MELO CFV MELO CM MELO EN MELO FP MELO KCM MELO RAM MELO RCN MELO RRS MELO TQ MENDES SP MENDES-DA-CRUZ FS MENDEZ-OTERO R MENDONÇA FAS MENDONÇA LM MENDONÇA PP MENDONÇA RZ MENESES GCM MENEZES GB MENI AZ MERLO S MERMELSTEIN CS Z - 31 D - 10 L - 3 N - 7 N - 12 N-8N-9 S-9 Q -4 G - 14 R -11 T - 22 N - 41 B – 9, B – 10, B 19 R -41 P - 34 A - 88 N - 15 G – 22, G - 26 A - 62 M -11 A - 12 A - 53 D-9 N - 17 B – 206, B - 208 P - 23 T - 53 C - 114 Z - 29 C-9 T-7 N – 20, N - 28 Z - 28 R -18 B – 43, B – 116, B – 222 T – 33, T – 36, T – 39, T – 41, T 51 G - 19 F-7 Z - 31 T - 34 X -6 C - 25 B - 143 B - 187 A - 103 C-8 A - 47 D – 25, D – 26, D – 31, D – 85, D – 89, D – 134, D - 135 A – 38, K – 11, K – 12, K – 13 A-6 B – 239, C - 14 C-1 A - 24 B - 228 C – 37, C - 38 Z - 41 T - 27 A – 61, S - 25 O -8 A - 78 B - 237 G - 13 Z - 19 G - 24 B - 87 H – 4, H – 11, T – 82, Z - 32 C - 88 O -1, O -2 A - 34 D – 54, D – 119, D – 132 C-7 R -34 B - 161 A – 89, C – 96, G - 33 R -55 P - 33 A - 29 D - 65 B - 134 K – 16, T - 28 P - 10 R -50 D - 20 G - 36 G - 22 C – 15, C – 28, C – 102, G - 5 S - 23 T – 15, T - 16 A – 81, H – 2, H – 3, N - 26 MESQUITA LV MESQUITA-FERRARI RA METZ C MIDLEJ VVP MIETTO BS MIMURA KK MIRAGLIA SM MIRANDA A MIRANDA DC MIRANDA F MIRANDA MASP MIRANDA NF MIRANDA-ALVES L MISSASSI G MOLINA RAS MOLOGNONI F MOLZ P MONESI N MONTE SM MONTE-ALTO COSTA A MONTEIRO AC MONTEIRO EC MONTEIRO JC MONTEIRO NS MONTEIRO VA MONTENEGRO RC MONTICO F MORAES AS MORAES CA MORAES GV MORAES IB MORAES JA MORAES JZ MORAES MNCM MORAES VWR MORAIS AS MORAIS BP MORAIS DB MORANDI FILHO R MORANDI V MOREIRA CPS MOREIRA JE MOREIRA MP MOREIRA RJP MORGADO-DÍAZ JA MORITA M MOROZ A MORRIS EAR MORTARA RA MOSCARDINI F MOTOYAMA AB MOTTA CM MOTTA LL MOTTA MCM MOURA DJ MOURA EG MOURA GEDD MOURA NETO V MOURA RS MOYSÉS GR MÜLLER CB MÜLLER YMR MURATA GM NADER HB NAGAI MA NAGAO PE NAKAMA KK NAKAYAMA ABS NASCIMENTO AR NASCIMENTO J NASCIMENTO LF NASCIMENTO LPS NASCIMENTO NG NASCIMENTO RD NASCIMENTO SC NASCIUTTI LE NATALI MRM NAVA A NAVES TB NAZARENO A NAZARI EM NDREWS NW NEGRI E NEILSON AL NEIVA M NETTO CA NETTO FSF E -5 C – 41, C - 56 B - 109 R -3 T - 22 C - 89 D – 6, D - 8 G-4 D – 85, D – 89, D – 134, D - 135 B - 221 B - 133 B - 68 B -66 D - 49 B -243 B - 30 A – 32, T - 40 N - 16 C - 91 C – 5, C - 12 O -9 V-2 A - 51 O -5 B - 188 B - 234 D - 96 A – 54, N – 23, O -7 T - 63 R -27 A - 58 C - 112 B – 250, C - 63 J - 41 G - 23 O -15 K-5 D – 25, D – 26, D - 27 D – 123, D - 127 B - 146 B-7 T – 70, T – 71, T – 15, T – 16, T 17 Z-3 B - 79 B – 50, B – 72, B – 76, B – 79, B 191 M -7 B - 26 H-5 R -2, R -36 B - 105 B - 187 A - 90 H - 13 A – 88, F – 25, F - 13 U-8 B -207, J - 43 J - 26 A – 106, B – 69, B – 106, B - 194 B -24 B - 148 C - 16 D – 61, N – 30, N – 39, N – 46 C - 47 B – 86, J - 26 B - 193 R -59 C - 103 T - 17 J - 29 B - 95 R -8 D - 94 C - 80 T – 5, T - 6 B - 223 A – 6, A – 77, B – 8, B – 105, C – 65, P - 18 T - 20 D - 138 T - 31 E -1 N - 38 V-9 C - 73 V-5 B - 224 G – 29, K - 4 J - 23 127 NEVES FMO NEVES LMG NEVES MM NEVES RL NICOLAU LAD NIERO ELO NISHAN U NISIMURA LM NITÃO ETGR NOBRE LTDB NOCE BPD NOCITI JUNIOR FH NOGUEIRA-MACHADO JA NORMANN CABM NOVAES RD NUNES MCOF NUNES PR NUNES VS NUÑEZ CEC NUSSENZVEIG HM OGIAS D OKADA FK OLALLA-SAAD S OLIANI SM OLIVA MLV OLIVA SU OLIVEIRA AC OLIVEIRA ACBF OLIVEIRA ADPR OLIVEIRA APS OLIVEIRA C OLIVEIRA CA OLIVEIRA CB OLIVEIRA CFA OLIVEIRA CJL OLIVEIRA DC OLIVEIRA EG OLIVEIRA EM OLIVEIRA F OLIVEIRA FC OLIVEIRA FP OLIVEIRA JA OLIVEIRA JC OLIVEIRA JG OLIVEIRA JS OLIVEIRA JSS OLIVEIRA LL OLIVEIRA LP OLIVEIRA ML OLIVEIRA MP OLIVEIRA PF OLIVEIRA PT OLIVEIRA RB OLIVEIRA RJS OLIVEIRA RL OLIVEIRA RR OLIVEIRA SBP OLIVEIRA SKM OLIVEIRA VA OLIVEIRA VCC OLIVER C ORIÁ RB ORNELAS IM ORTOLANI-MACHADO CF OSAKI JH OSAKI LH OSORIO LKP OTA CCC OURIQUES LC PAÇÓ-LARSON ML PADRÃO JC PÁDUA TA PAFFARO AMA PAFFARO JUNIOR VA PAIVA CAL PAIVA PMG PALLADINO MV PALUDO KS PANZETTA-DUTARI GM PAOLI F PAPA MP PAREDES BD PAREDES-GAMERO EJ PARISE CB PARK C-M PARK H-S N - 10 P - 10 D – 33, D - 34 B - 235 C – 37, C - 38 B -192 A - 42 A - 39 N – 9, N - 12 B - 139 A - 99 P - 23 J – 18, J - 23 L – 2, L - 4 R -15, R -16 N - 13 C – 27, F - 16 A-3 J - 48 S-8 F - 17 D-8 B – 122, B – 126, J-4 B – 41, B – 83, C – 3, C – 52, C – 71, C - 89 B – 91, B - 136 D-6 R -38, R -64 A - 106 S - 16 A - 22 C – 2, D – 2, D 4 D – 32, D – 47, D – 48, D – 75, D – 76 B -160 D - 36 J - 28 C - 11 B-8 U - 38 X -2, X - 6 R -74 N - 43 J - 32 S-3 D – 104, M -8 D - 132 R -40 B -48, B – 129, S - 12 P – 36, P - 37 E -11 B - 220 Z - 29 H – 19, M -11 F-8 B - 19 D - 76 C - 65 H - 12 C - 106 B - 149 Z - 30 A – 23, A - 24 C – 49, C – 50, C – 51, T - 46 T - 80 D - 78 M -4 G – 14, J - 39 U - 13 G – 35, G - 37 U – 23, U – 28, U - 38 A – 11, A – 14, A – 15, A - 27 R -49 C - 64 D – 84, D – 87, D – 101, D – 102, D – 111, D - 114 D – 35, D – 56, D – 62, D - 81 T - 10 C - 111 B - 190 R -45 A - 55 B -16 G - 18 A - 94 G – 4, Z - 4 C - 63 Z - 42 A - 20 PARREIRA GG PASSOS JL PASSOS LAC PATRICIA PRANKE P PAUL AL PAULA ACC PAULA CAA PAULA JUNIOR R PAULA SO PAULSEN BS PAZ AHR PECLI E SILVA C PEDREIRO MRD PEDRO AN PEDROSA CSG PEIXOTO AR PEIXOTO BC PELAJO-MACHADO M PENNACCHI PC PENTEADO MV PERDE-SCHREPLER M PEREIRA BF PEREIRA CG PEREIRA CN PEREIRA DA PEREIRA DT PEREIRA EMR PEREIRA GB PEREIRA JAL PEREIRA JRCS PEREIRA LA PEREIRA LX PEREIRA MC PEREIRA MJB PEREIRA MLG PEREIRA NP PEREIRA RFC PEREIRA RVS PEREIRA VS PEREIRA-NEVES A PERERIA NP PEREZ AM PEREZ APS PEREZ DA PEREZ MO PERINI VR PEROTTI TL PERUQUETTI RL PESSOA AFM PETTENUZZO LF PFAFFENSELLER B PIAZZA FV PIECHNIK CA PIEDADE WP PILATTI FK PIMENTA MT PIMENTEL ER PINHAL MAS PINHATTI AV PINHEIRO APB PINHEIRO CR PINHEIRO NM PINHEIRO PFF PINHO CF PINHO V PINTO JS PINTO ME PINTO MT PINTO NCS PIRES BRB PIRES DA PIRES NPMD PIZZATTI L PIZZOL JÚNIOR JP PLIEGO CM POEYS SC POITOU C POLON L POLTRONIERI AB PONTES B PONTES CLS PORCIONATTO MA PORTILHO NA PÔRTO LCMS PORTUGAL CC POSER GV POSSIDONIO ACB POSSUELO L POZZI R PRADO JL PRADO KM PRADO LCS PRADO PF A - 43 D - 84 D - 105 Z – 6, Z – 7, Z – 8, Z – 10, Z – 9, Z – 11, Z – 12, Z – 14, Z – 39 F - 12 Z - 37 B - 136 C - 72 C - 29 Z - 36 Z - 25 I-8 A - 92 B – 13, H - 15 G - 11 D - 99 O -18 N – 41, N - 22 P - 19 P-9 B - 59 G-3 B - 247 P - 22 B - 144 U - 23 T - 68 A - 11 A - 113 B - 34 T-9 A - 43 D-5 G-8 D - 93 D - 22 R -46 C - 34 L-5 R -4 D – 16, D - 19 F - 21 D - 128 C - 19 P - 16 D - 71 P-8 D - 83 C - 90 T - 30 G - 34 T – 76, T - 78 A - 13 A - 37 U - 20 D - 109 P – 15, P – 16, P – 28, P – 36, P – 37 A – 34, P - 20 B - 64 D – 60, D - 79 T - 73 H-7 Z-2 D - 37 C – 13, C – 18, C – 79, C – 81 B - 169 D - 69 J - 19 A - 87 B - 117 C - 28 N - 19 B - 65 G - 32 B - 163 R -44 C - 114 N - 50 C - 52 S-8 B - 157 A – 59, I – 3, I - 4 N - 22 C - 101 C - 78 B - 64 H-2 O -6 F-2 A - 93 D - 63 A - 33 R -70 PRADO PS PRECUP C PREDES FS PRETA CMCC PRIOR I PROCÓPIO MS PRUSCH DS PRZEPIURA TCS PUGA CCI PULEGIO M PUTTI JS QUEIROZ AC QUEIROZ FR QUEIROZ MS QUINTANA CYP QUINTÃO JLD QUINTAR AA QUINTILIANO K QUONDAMATTEO F RABELO K RABELO SM RABINOVITCH M RACHID CVM RAHAL P RAMÃO A RAMOS AB RAMOS BCR RAMOS CA RAMOS GOR RAMOS MSC RAMOS RGP RAMOS TCP RANGEL LBA RAPOZO-YOUNES V RASIA-FILHO A RAULINO JCN REAL FRO REBELATO HJ RECCO-PIMENTEL SM REGO EM REGO LNAA REHEN SK REIS AC REIS CF REIS DD REIS DDA REIS IDG REIS INRS REIS MC REIS PA REIS RM RENNER GDR RESENDE VTR REZENDE BM REZENDE KF REZENDE-TEIXEIRA P RIBAS VT RIBEIRO AAGFC RIBEIRO AF RIBEIRO AHR RIBEIRO DA RIBEIRO DL RIBEIRO FILHO AC RIBEIRO FILHO J RIBEIRO FM RIBEIRO HJ RIBEIRO JA RIBEIRO JU RIBEIRO LHG RIBEIRO LM RIBEIRO NM RIBEIRO PC RIBEIRO RR RIBEIRO VMA RICARDI LR RIEDERER I RIEGER A RIETDORF JM RINALDI JC RIVAROLI L RIVAROLI L RIVERO ERC RIZZO E ROBBS BK ROBERT AW ROCHA CB ROCHA GG ROCHA HAO ROCHA LO ROCHA SC ROCHAEL NC RODARTE RS RODRIGUES AA RODRIGUES APD D – 58, D - 59 B - 59 D - 95 F - 25 J - 12 A - 107 B - 155 G-9 D-7 D - 41 D - 57 B - 145 R -12 A - 27 P - 18 C - 102 B - 21 Z - 11 B - 119 S - 20 P - 29 R -21 B - 134 B – 35, B - 36 B - 248 K - 16 A - 120 A - 122 P - 25 C – 45, C – 46, C – 103 A - 112 A - 101 B – 85, B – 198, B – 232 T - 79 T-8 A – 121, A – 122 R -2 D-1 D - 52 B - 179 A - 95 Z - 36 C - 18 B - 102 T – 5, T – 6, T - 7 C-9 S - 17 U - 31 C - 42 C - 74 B – 9, B - 10 U - 15 K-2 C - 81 A - 115 N - 18 F - 19 V-2 V – 3, V - 8 N-1 B – 6, F - 2 D - 38 B - 13 C - 95 T - 67 S-5 A - 35 B - 226 D - 29 C - 116 X -1 G - 25 A - 36 R -19 A - 10 I – 11, P - 31 S - 27 S - 29 B - 216 R -65 R -75 P - 25 D – 44, D – 46, D – 58, D – 59, D – 60, D – 71, D – 79, D - 116 G – 39 Z - 34 E -5 B - 37 B - 139 D - 125 B - 44 R -37 B – 205, B – 227 J – 38, R -31 A - 109 128 RODRIGUES BR RODRIGUES C RODRIGUES FV RODRIGUES HA RODRIGUES HF RODRIGUES HF RODRIGUES JCF RODRIGUES LP RODRIGUES MD RODRIGUES ML RODRIGUES PC RODRIGUES PMGRS RODRIGUES T ROESLER R ROMAN SS ROMANA-SOUZA B ROMÃO LF RONDON AMR ROQUE NR ROSA AR ROSA AS ROSA HT ROSA VS ROSAS EC ROSI MID ROSS BH ROSSI A ROSSI MID ROSSI PIVA MB ROZENTAL S RUANO RM RUIZ AC RUIZ RC RUIZ TRG RUMJANEK VMBD SABA GT SABATINO ME SABINO PM SADDI TM SAEZ RC SAFFI J SAITO A SALDANA-CABOVERDE A SALES CF SALGADO LT SALLES ESL SALLES GN SAMPAIO MC SANCHES BDA SANCHES D SANGIULIANO B SANT’ANA FILHO M SANT’ANNA C SANTANA DB SANTANA MAN SANTANA PT SANTOS AA SANTOS AB SANTOS ABG SANTOS ACV SANTOS AMF SANTOS AO SANTOS BS SANTOS CA SANTOS CLP SANTOS DC SANTOS DO SANTOS DS SANTOS E SILVA SV SANTOS EM SANTOS ESJ SANTOS EV SANTOS FCA SANTOS HB SANTOS HCP SANTOS IGD SANTOS JMCO SANTOS JMP SANTOS JN SANTOS JR AR SANTOS JS SANTOS LL SANTOS LM SANTOS LPB SANTOS MA SANTOS MB SANTOS MF SANTOS MF B - 116 K-9 A - 66 T-2 N - 23 A - 54 A - 93 K-4 B - 223 R -28 B - 28 N - 20 G – 16, G – 20, G – 23, G – 24, G – 25, G - 27 B – 4, B – 155, B – 196, B – 230, B – 231, K - 10 A – 68, C – 84, C – 87, D – 103, D – 138 P – 3, P - 12 B - 128 B - 159 R -61 Z - 10 P-3 C – 4, O -6, U - 8 D - 12 C - 64 H – 14, J – 30, N -4 V-7 K-7 B – 159, B – 211, C – 54 P - 20 A – 82, R -30 D - 62 K-2 R -34 B - 212 B – 47, B – 55 D - 13 F - 12 B - 215 D - 110 B - 57 B – 152, B – 202, B – 203, B – 204, B – 233 J - 21 N - 36 D - 112 U - 39 D - 56 B - 84 C - 53 A - 104 R -38 B - 184 B - 150 U-6 B - 170 B - 229 C - 94 T - 47 R -13 C - 33 A-9 C - 50 I - 11 S-6 B - 246 A - 100 D - 77 B - 175 C – 98, Z - 21 D - 122 D - 48 B - 47 T - 24 D - 110 A – 41, A – 76, D – 112, D - 115 D - 39 N - 25 B - 63 C - 61 F-5 A – 2, E -3 C - 12 C – 118, L - 3 B - 99 A - 82 R -33 O -17 C - 90 I – 9, I - 10 SANTOS MM SANTOS MO SANTOS MR SANTOS MRV SANTOS MT SANTOS NF SANTOS PCF SANTOS RS SANTOS RT SANTOS TCP SANTOS TG SANTOS TM SANTOS VM SANTOS VT SANTOSS DS SARAIVA EM SARAN PS SARNO EN SASSI FA SASSONE-CORSI P SAVINO W SCABORA JE SCARANO WR SCARDUA PMS SCHECHTMAN D SCHENKMAN S SCHER CR SCHERHOLZ PLA SCHICHOR CH SCHMIDT EC SCHNEIDER N SCHOFFEN JPF SCHULTZ TH SCHULTZE E SCHUNEMANN M SCHUPBACH G SCREIBER AZ SCUDELER EL SEABRA SH SEDMAK B SEGURA-VALDEZ ML SEIXAS FK SEONG-HO J SERACHI FO SERRÃO JE SERVATO JPS SEVERI-AGUIAR GDC SHIMABUKURO MK SHINOHARA EMG SIGNORETTI PVP SILVA AA SILVA AAN SILVA ACS SILVA AH SILVA AN SILVA AO SILVA AP SILVA AR SILVA ASF SILVA BH SILVA BJM SILVA CG SILVA CJ SILVA CL SILVA CM SILVA CV SILVA DC SILVA DD SILVA DF SILVA DJ SILVA DM SILVA DRA SILVA EAM SILVA EL SILVA EML SILVA EO SILVA ERM SILVA FBF SILVA FG SILVA FILHO ACC SILVA FP SILVA FR SILVA GAB SILVA GET SILVA GF SILVA GHC SILVA GM D - 47 D - 27 F-7 A-9 P - 30 G - 21 A - 40 D – 111, R -59, U -2 T - 83 R -35 T - 39 A – 71, D - 97 G - 20 A - 87 Z-9 J – 35, R -37 B - 39 L-5 B-4 D - 83 C – 83, I – 13, P 31 N - 14 D – 17, D – 37, D – 45, D – 97, D – 99 B - 62 A - 25 A – 101, A – 102 Z-7 D - 88 Q -2 U – 24, U – 14, U - 28 Z - 25 E -1, E -2, T - 20 P - 27 B - 213 U - 25 J - 45 S - 30 Z-2 R -24 T- 85 A - 118 B - 225 J - 17 B - 177 N – 3, V - 1 B - 11 D - 14 T – 82, Z - 32 O -5 Z-1 R -51 D - 119 B - 142 A - 72 C - 13 B – 114, B – 218 B - 120 D - 136 K-6 B - 205 R -66 A - 97 J – 31, U - 42 B - 101 T - 13 A – 40, A – 53, J – 38, R -31, R 39, R -51, R -70 E -9 B - 140 B -24 N - 27 T - 61 G-6 R -14, R -18 J - 25 V - 10 A – 109, A - 111, A - 113, A - 114, A – 116, M -12, R -66, R – 72, U 10 X -3 B - 164 C - 45 B - 27 B – 240, B – 242, O -12 T - 23 N – 25, S - 17 M -5 F-9 D - 75 F - 10 SILVA IDCG SILVA JAF SILVA JG SILVA JH SILVA JPV SILVA JR FP SILVA JRMC SILVA JÚNIOR RMP SILVA KA SILVA KEF SILVA KR SILVA LCF SILVA LLP SILVA LLR SILVA LM SILVA LM SILVA LS SILVA MA SILVA MBB SILVA MCC SILVA MMM SILVA MRD SILVA MS SILVA MT SILVA NETA HL SILVA NM SILVA NS SILVA PCM SILVA PF SILVA RB SILVA RC SILVA RF SILVA RJ SILVA RR SILVA RRP SILVA SA SILVA SG SILVA SV SILVA TA SILVA TG SILVA TKA SILVA TM SILVA TP SILVA VMM SILVA WLB SILVA-NETO A SILVEIRA BS SILVEIRA GF SILVEIRA LTR SILVEIRA MS SILVEIRA NM SILVEIRA PF SILVEIRA-LACERDA EP SILVESTRIN RB SIQUEIRA AS SIQUEIRA EE SMAILI SS SMANIOTTO S SMANIOTTO S SMITH RKW SMUCZEK B SOARES BM SOARES CP SOARES CP SOARES FA SOARES FAF SOARES FLF SOARES HM SOARES JM SOARES MAM SOBRINHO MF SOBRINHO PHG SOCODATO R SOGAYAR MC SOGAYAR MC SOLETTI RC SOMASUNDARAM K SONEHARA NM SONODA MT SOSTHENES MCK SOUSA AL SOUSA CEC SOUSA CM SOUSA LP B – 77, B – 246, J – 14, J – 36, O 10 I-6 B - 82 A - 77 C - 26 B - 28 A – 115, B – 120, B – 121, I – 5, S 26 B - 161 B - 32 A – 110, E -12 Z - 20 P - 14 V - 10 R -57 B – 27, B – 33, C -7 O -3, R -12, R -5, Q -1, R -6, S – 10, Z - 3 T - 84 D - 136 A - 44 B - 91 P - 17 O -11 F - 18 C - 56 D - 10 R -41 G – 10, Z - 18 U-5 N-6 T - 60 C – 94, C – 116, J - 47 A - 41 D-5 B - 241 M -12 D - 38 N-8 B – 73, C – 117 B - 168 B – 180, C – 106 X -2 T - 81 G - 33 B – 114, B – 118, B – 185, B – 201 H-9 R -5, R -6 B - 110 B - 147 D - 45 F - 23 U - 34 A - 119 B - 210 T - 44 B – 125, B – 167 B - 141 G - 13 A – 30, P – 21, P – 24, P – 33 U 29 Z - 38 B - 74 B – 234, U - 4 A – 7, B – 84, B – 141, B – 142, B – 182, B – 183, H 3 B - 39 S-4 U – 40, U - 41 L-6 B - 128 G-9 B - 90 A - 70 C - 76 B - 70 K – 14, Z - 5 C - 93 B - 46 C - 71 C - 46 N – 27, T – 26, T - 48 D - 80 C - 113 A - 48 C – 26, I - 2 129 SOUZA AC SOUZA ACF SOUZA BC SOUZA BK SOUZA BL SOUZA CRB SOUZA EE SOUZA FN SOUZA FS SOUZA IC SOUZA JM SOUZA JR PF SOUZA KS SOUZA LEL SOUZA LM SOUZA LOJ SOUZA MC SOUZA MVR SOUZA NETO CPS SOUZA NHC SOUZA PAF SOUZA RB SOUZA SR SOUZA TA SPOHR TCLS STEFFENS D STERNBERG C STIMAMIGLIO MA STREIT JR DP STUMPP T STUR E SUZUKI IL TABOGA SR TAMIR S TANIWAKI NN TANOWITZ HB TARQUINIO SBC TAVARES ALP TAVARES E SILVA R TAVARES MG TEIXEIRA AD TEIXEIRA BC TEIXEIRA BL TEIXEIRA C TEIXEIRA CFP TEIXEIRA FR TEIXEIRA L TEIXEIRA LCM TEIXEIRA MM TEIXEIRA S TEIXEIRA SC TENÓRIO DPLA TERSARIOL ILS TESSER RB E -10 D - 33 C - 48 B – 230, B – 231 E -6 R -56 J-5 S - 11 E -7 A - 21 B - 80 D - 102 C – 44, D - 40 E -2 B - 233 T - 50 C - 109 B - 18 B - 145 C - 62 S - 10 S-7 U - 26 O -7 B - 69 Z - 12 B – 162, B – 188 Z - 34 N - 31 N – 5, N – 6 B - 92 F - 27 A – 70, A – 104, D – 7, D – 9, D – 23, D – 51, D – 53, D – 100, D – 128, D – 133, D – 137, N - 21 S - 13 C - 33 R -26 B - 169 N-2 X -9, X -10 S-4 N-3 C - 48 T - 41 C – 53, C – 57, C – 59, C – 80, C 104 C - 82 X -4 C - 97 F - 24 C – 23, C – 24 T - 37 R -39 S-6 J - 33 N-5 THOMÉ R TIAN X TODESCHINI AR TOLEDO DAM TOLEDO PC TOMIOSSO TC TOMIYAMA L TONELLI FMP TONI KLG TONIAZZO AP TORRES AFC TORRES AMH TORRES DNM TOSO VD TRAVA-AIROLDI VJ TRAVASSOS R TRINDADE GS TURCK P TURRI JAO UEIRA-VIEIRA C URIAS U UTIYAMA AH VAGO AR VALADÃO PAC VALENCA SS VALENTE SF VALIM CXR VALSECHI MC VARELA JN VAROTTI FP VASCONCELOS RO VECCHI L VEIGA SS VENDITE D VENTURA ALM VERAS PST VERÇOZA BRF VERGARA FMF VERINAUD L VERSUTE EM VIANA AM VIANA IMMN VIANA MN VIANI FC VIANNA MCB VIAU CM VICENTINI CA VICTONI T VIDAL RS VIEGAS GS VIEIRA AM VIEIRA JRC VIEIRA LB VIEIRA LFA VIEIRA LN C - 20 J-1 N - 45 R -55 O -14 P - 28 B - 96 Z - 17 D - 65 G - 28 G - 26 B - 138 Z - 40 A - 28 A-4 S - 24 B – 124, H - 8 Z - 24 B - 199 D - 121 B - 193 V-3 B – 32, B – 42, B - 54 T-1 C - 88 R -14 R -25 B - 35 R -20, R -42 B - 34 B - 124 B - 174 A – 61, A – 86, A – 103, P – 5, S – 11, X -7 T - 66 T – 80, T - 81 R -63, R -69, R 71 R -60 R -52 A – 5, C - 20 D - 90 U - 20 P - 24 C - 82 T - 54 A - 14 B – 202, B - 203 N – 44, N – 47, N - 49 A - 80 B - 154 C - 17 B - 118 A – 110, B – 229, E – 12 J - 44 P – 21, U - 29 U – 21, U - 22 VIEIRA NETO JP VIEIRA PC VIEIRA TSS VILAÇA JA VILANOVA-COSTA CAST VILARDO AFRM VILELA ALM VILELA RC VILLA-VERDE DMS VILLODRE ES VIOLA JPB VIOTTO AC VOLPE CMO VOTTO APS WARD LS WATANABE I-S WEINGRILL RB WEIS SN WEITKUNAT M WERNECK CC WILLE ACM WINK MR WIPPICH AC WOO JC WOSNIAK JÚNIOR J WOZNIAK RW XAVIER GF XAVIER JM YAMANOUYE N YAN CYI YONG-HO A YOO J-O YU-PEI C ZACARO AA ZAMBONI F ZAMIN LL ZAMPONI GW ZANAROTTI GM ZANETTI BF ZANIER-GOMES PH ZANON RG ZAVAN B ZEBDA N ZEIDLER JD ZENI EC ZENKNER FF ZINGALI RB ZOGOVICH M ZORÉL VJ ZORN TNT ZUCCARI DAPC ZULIAN JG ZUMA AA ZÚÑIGA HAU ZUZA AL ZUZZI DC ZYCH J U-4 C - 68 J - 35 B - 111 B - 210 U - 36 B - 22 U-6 C - 98 B - 89 G – 39 G - 19 J - 18 B - 123 B - 102 T - 62 D - 139 G – 28, G - 29 N - 24 P - 22 A - 86 A – 69, B – 135, B – 244, K – 9, Z - 33 R -45 Z - 42 J - 24 B – 71 F - 24 B - 126 B – 81, J – 3, J - 7 N-19 J - 17 B - 53 G-2 B - 51 Z - 14 B - 23 J - 44 N - 32 K-3 T - 58 T - 24 D - 35 J-2 F-1 N - 38 S - 27 B - 144 S - 14 P-6 P-4 B – 100, B – 101, B - 103 H-1 F - 13 A - 117 D - 130 P-1 O -13 130 Highlights: Keynote Symposium by Steven Chu, U.S. Secretary of Energy, and Arthur D. Levinson, Chair, Genentech, Inc., and Apple, Inc. Threads (meetings-within-a-meeting):1) Cell Biology and Medicine and 2) Cell Biology and the Physical Sciences. Daily programs will allow attendees to follow new fields while benefitting from a large meeting with the best research in cell biology. World-class Symposia and Minisymposia speakers Frontier Symposia will synthesize current, exciting progress in the field Science Discussion Tables—interact with senior scientists in an intimate setting Posters, mentorship, networking, career development, and education programs Fun in the wonderful city of San Francisco! Important deadlines July 23, 2012 (Application Deadline) Special Interest Subgroup July 30, 2012 (Application Deadline) Abstract submission (for Minisymposium or poster consideration) September 4, 2012 (Application Deadline) Regular abstract submission (poster only) October 10, 2012 Early registration deadline www.ascb.org 131
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