Development and application of a biological assessment systems Multimetric Assessment of macroinvertebrates and Assessment Software Dr Mario Sommerhäuser and University of Essen Institute of Ecology Department of Hydrobiology Multimetric Assessment and Assessment Software Biological Assessment CETEC Curso “Aplicação de Ecomorfologia Fluvial na Validação da Tipologia de Rios” 16-18/06/2010 Multimetric Assessment and Assessment Software Main stressors for rivers today (Europe) Organic pollution Eutrophication Hydromorphology CETEC Curso “Aplicação de Ecomorfologia Fluvial na Validação da Tipologia de Rios” 16-18/06/2010 Multimetric Assessment and Assessment Software Indicator value of the different biological quality components Site Organic pollution Basin Eutrophication Acidification „Holistic approach of WFD“ HydroMorphology/ Migration short-term reaction Long-term reaction CETEC Curso “Aplicação de Ecomorfologia Fluvial na Validação da Tipologia de Rios” 16-18/06/2010 Multimetric Assessment and Assessment Software Common features of the new German biological assessment methods for all four aquatic quality components Type-specific approach Assessment in comparison with reference conditions (~ high ecological status) New and specific sampling methods Multimetric approaches for the analysis of biological results (i. e. species lists) - combination of different metrics (biocoenotic indicator values/functional groups): e. g. species number, share of certain groups like Stoneflies and others, share of functional feeding groups) - Different reaction againts different stressors - CEN and ISO standards for European biological indices CETEC Curso “Aplicação de Ecomorfologia Fluvial na Validação da Tipologia de Rios” 16-18/06/2010 Multimetric Assessment and Assessment Software Example for the assessment with biol. quality elements: Macroinvertebrates AQEM / PERLODES system CETEC Curso “Aplicação de Ecomorfologia Fluvial na Validação da Tipologia de Rios” 16-18/06/2010 Multimetric Assessment and Assessment Software Content Content General concept and development of the multimetric assessment system (AQEM / PERLODES, Germany) Design and application European River Classification Software System Version 3.0 Interpretation of assessment results Examples of taxa, ecological information, species traits, indicative potential as a basis for multimetric assessment systems CETEC Curso “Aplicação de Ecomorfologia Fluvial na Validação da Tipologia de Rios” 16-18/06/2010 8 Multimetric Assessment and Assessment Software General Concept Forms of impairments (“Stressors”) Nutrient enrichment/ organic pollution Hydromorphology Land use Microhabitats CETEC Curso “Aplicação de Ecomorfologia Fluvial na Validação da Tipologia de Rios” 16-18/06/2010 9 Multimetric Assessment and Assessment Software General Concept Ecological Quality Assessment: Multi-metric concept „Ecological Status“ Stagnation Function Acidification Global warming Resistence Poison Hydromorphology Resilience Diversity Fine sediments Trophic structure Species richness Pollution Salt Sensitivity CETEC Curso “Aplicação de Ecomorfologia Fluvial na Validação da Tipologia de Rios” 16-18/06/2010 10 Multimetric Assessment and Assessment Software General Concept Stressor-specific Assessment Reference status Information on the saprobic status Taxalist Information on the acidic status Information on other stressors Assessment formula Assessment formula Ecological status Assessment formula Need for action CETEC Curso “Aplicação de Ecomorfologia Fluvial na Validação da Tipologia de Rios” 16-18/06/2010 11 Multimetric Assessment and Assessment Software General Concept Stressor-specific Assessment Reference status Assessment Modul organic pollution formula Information on the saprobic status Taxalist Information on the acidic status Information on other stressors Assessment formula Modul acidification Ecological status Assessment formula Modul general degradation Need for action CETEC Curso “Aplicação de Ecomorfologia Fluvial na Validação da Tipologia de Rios” 16-18/06/2010 12 Multimetric Assessment and Assessment Software General Concept Stressor-specific Assessment Taxalist Information on the saprobic status German Standard DIN 38410 (2004) Information on the acidic status Acid quality classes according to Braukmann & Biss (2004) Information on other stressors Stream type specific assessment - formulas: Metric 1 Metric 2 Metric 3 ... CETEC Curso “Aplicação de Ecomorfologia Fluvial na Validação da Tipologia de Rios” 16-18/06/2010 13 Multimetric Assessment and Assessment Software General Concept Stream type-specific Saprobic Index Example: Saprobic Index = 1.85 reference good moderate poor bad Alpine streams < 1.20 to 1.80 to 2.55 to 3.25 to 4.00 Small mountain streams < 1.45 to 2.00 to 2.65 to 3.35 to 4.00 Mid-sized mountain streams < 1.60 to 2.10 to 2.75 to 3.35 to 4.00 Mid-sized sand bottom streams in the lowlands < 1.90 to 2.35 to 2.90 to 3.45 to 4.00 (...) CETEC Curso “Aplicação de Ecomorfologia Fluvial na Validação da Tipologia de Rios” 16-18/06/2010 14 Multimetric Assessment and Assessment Software General Concept Stressor-specific Assessment Taxalist Information on the saprobic status German Standard DIN 38410 (1992) Information on the acidic status Acid quality classes according to Braukmann & Biss (2004) Information on other stressors Stream type specific assessment - formulas: Metric 1 Metric 2 Metric 3 ... CETEC Curso “Aplicação de Ecomorfologia Fluvial na Validação da Tipologia de Rios” 16-18/06/2010 15 Multimetric Assessment and Assessment Software General Concept Stressor-specific Assessment Taxalist Information on the saprobic status German Standard DIN 38410 (1992) Information on the acidic status Acid quality classes according to Braukmann & Biss (2004) Information on other stressors Stream type specific assessment - formulas: Metric 1 Metric 2 Metric 3 ... CETEC Curso “Aplicação de Ecomorfologia Fluvial na Validação da Tipologia de Rios” 16-18/06/2010 16 Multimetric Assessment and Assessment Software What is a Metric? - Abundance of a mayfly species General Concept Multi-metric Index (Hering et al. 2004a, b; Lorenz et al. 2004; Meier et al. 2005) - Number of mayfly species - Percentage of all mayfly species on the sample - Presence of specific indicator species of the mayflies - Percentage of the filter feeding organisms CETEC Curso “Aplicação de Ecomorfologia Fluvial na Validação da Tipologia de Rios” 16-18/06/2010 17 Multimetric Assessment and Assessment Software General Concept Selection of Metrics Database 1 Taxalists Calculation of ca. 80 metrics with each taxalist Correlation analysis Database 2 Abiotic site data - land use in the catchment - hydromorphological quality Selection of parameters, which indicate the effects of degradation CETEC Curso “Aplicação de Ecomorfologia Fluvial na Validação da Tipologia de Rios” 16-18/06/2010 18 Multimetric Assessment and Assessment Software General Concept Metric results & Land use parameters Correlation Analysis Metric results & hydromorphological parameters CETEC Curso “Aplicação de Ecomorfologia Fluvial na Validação da Tipologia de Rios” 16-18/06/2010 19 Multimetric Assessment and Assessment Software General Concept Mid-sized sand bottom streams in the lowlands Anzahl Taxa Trichoptera of Trichoptera Number 20 16 12 8 4 0 0 2 4 6 8 10 Residential Siedlungarea [%] [%] CETEC Curso “Aplicação de Ecomorfologia Fluvial na Validação da Tipologia de Rios” 16-18/06/2010 20 Multimetric Assessment and Assessment Software General Concept Mid-sized sand bottom streams in the lowlands German IndexTyp 15 FaunaIndex GermanFauna 1,8 1,2 0,6 0,0 -0,6 -1,2 -1,8 0 2 4 6 8 10 Siedlung area [%] [%] Residential CETEC Curso “Aplicação de Ecomorfologia Fluvial na Validação da Tipologia de Rios” 16-18/06/2010 21 Multimetric Assessment and Assessment Software General Concept Selection of Metrics Calculation of ca. 80 metrics with every taxalist Correlation analysis Selection of Candidate metrics Selection of parameters, which indicate the effects of degradation CETEC Curso “Aplicação de Ecomorfologia Fluvial na Validação da Tipologia de Rios” 16-18/06/2010 22 Multimetric Assessment and Assessment Software General Concept Criteria for the selection of the metrics High and significant correlation of a metric with hydromorphological or land use parameters Candidate metrics for each stream type cover all metric groups according to the WFD • taxonomic composition and abundance • richness/diversity • sensitive/tolerant taxa • functional CETEC Curso “Aplicação de Ecomorfologia Fluvial na Validação da Tipologia de Rios” 16-18/06/2010 23 Multimetric Assessment and Assessment Software General Concept Example: Mid-sized mountain streams Metric type Metric Information Composition EPT [%] % of sensitive taxa and habitats Richness/diversity No. of EPTCBO Diversity of fauna and habitats Sensitivity/tolerance German Fauna Index mid-sized mountain streams Presence of specialists for rare or degraded habitats Functional groups Metarhithral [%] Situation in the catchment CETEC Curso “Aplicação de Ecomorfologia Fluvial na Validação da Tipologia de Rios” 16-18/06/2010 24 ASTERICS Design and application of the AQEM/STAR European River Classification System v3.0 Multimetric Assessment and Assessment Software ASTERICS v.3.0 The current version of the software to calculate the ecological status of streams and rivers is downloadable for free! Homepage: - www.aqem.de or - www.fliessgewaesserbewertung.de Options: - Germany official system (PERLODES) - Germany original (AQEM system) - Several European countries CETEC Curso “Aplicação de Ecomorfologia Fluvial na Validação da Tipologia de Rios” 16-18/06/2010 26 Multimetric Assessment and Assessment Software ASTERICS v.3.0 Overview The assessment-software runs under Windows 98, NT, 2000 and XP. The data import is normally carried out via MS Excel. The import table must follow certain rules on layout and format. The assessment-software calculates the ecological quality status of each imported taxalist. The assessment-software calculates about 300 additional metrics for each imported taxalist. Note: Yet, no interpretation of the results or recommendations for improvement of the quality status are given. CETEC Curso “Aplicação de Ecomorfologia Fluvial na Validação da Tipologia de Rios” 16-18/06/2010 27 Multimetric Assessment and Assessment Software ASTERICS v.3.0 Data sources - Data import by Excel- or text-files (ASCII-Format); respect correct format - Main component of the data import: Key code (encoding every taxon definitely by numbers and/or letters) Background for encoding - Taxon names can have different spellings. - Taxon names are not enduring – they change often in time according to improvements in taxonomy. Thus, taxa could have different names in different databases (synonyms). - A key code is composed of identification numbers, which are assigned to every taxon (definite identification, independent of spelling or synonymy). - Codes could be made of numbers, letters or combinations of those. CETEC Curso “Aplicação de Ecomorfologia Fluvial na Validação da Tipologia de Rios” 16-18/06/2010 28 Multimetric Assessment and Assessment Software ASTERICS v.3.0 Application of the Assessment Software (in four steps) (1) Import: Import of an Excel-file or ASCII-file (2) Settings: choice of key code (3) Settings: choice of stream type and stressor Note: The menues 2 and 3 appear gradually after pressing the “Import” button. CETEC Curso “Aplicação de Ecomorfologia Fluvial na Validação da Tipologia de Rios” 16-18/06/2010 29 Multimetric Assessment and Assessment Software ASTERICS v.3.0 Application of the Assessment Software (4 steps) (1) Import: Import of an Excel file or ASCII file (2) Settings: choice of key code (3) Settings: choice of stream type and stressor (4) Start of calculation CETEC Curso “Aplicação de Ecomorfologia Fluvial na Validação da Tipologia de Rios” 16-18/06/2010 30 Multimetric Assessment and Assessment Software ASTERICS v.3.0 Taxalist ID_ART 4335 4380 4397 4419 4521 4584 4639 4642 5059 5075 5131 5237 5601 5605 5790 5851 5854 6468 6510 6780 6853 6886 6909 6972 6977 8142 8691 8736 8831 12091 12121 19110 TAXON_NAME Sieg Aphelocheirus aestivalis 0,8 Baetidae Gen. sp. 4,4 Baetis fuscatus 41,5 Baetis sp. 31,3 Caenis luctuosa 4,4 Ceraclea sp. 2,2 Cheumatopsyche lepida 82,9 Chironomidae Gen. sp. 24,0 Ecdyonurus venosus-Gr. 29,4 Eiseniella tetraedra 13,1 Serratella ignita 30,0 Leuctra geniculata 2,2 Hydropsyche pellucidula 15,3 Hydropsyche sp. 35,7 Leuctra sp. 10,9 Limnius opacus Lv. 102,5 Limnius volckmari Lv. 2,2 Polycentropus flavomaculatus 6,5 Potamanthus luteus 16,3 Rhyacophila sp. 15,3 Simulium sp. 268,4 Sphaerium sp. 2,2 Stenelmis canaliculata Lv. 48,0 Tanypodinae Gen. sp. 39,3 Tanytarsini Gen. sp. 19,6 Hydropsyche incognita 10,3 Asellus aquaticus 24,0 Oligochaeta Gen. sp. 8,7 Turbellaria Gen. sp. 2,2 Limnius opacus Ad. 9,5 Stenelmis canaliculata Ad. 2,2 Rhyacophila dorsalis/nubila 13,9 Results of the metrics Metrics sin sgn 2 0 2,1 0 2 2,1 2,1 0 1,5 0 1,9 1,6 2 0 1,5 0 1,6 2 2,1 0 0 0 1,8 0 0 2 2,7 0 0 0 1,8 0 1,90 zeu 4 0 4 0 4 4 8 0 8 0 4 16 4 0 8 0 8 4 8 0 0 0 4 0 0 4 4 0 0 0 4 0 zhy zer zmr 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 1 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 0 0 1 5 2 1 0 1 0 3 0 1 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0,1% 0,7% 3,7% zhr 0 1 1 2 1 0 0 1 3 2 3 0 2 0 2 3 3 2 0 0 0 0 1 0 0 5 1 0 0 3 1 0 zep 2 2 4 2 2 0 3 1 2 2 3 2 4 0 2 5 5 2 0 0 0 0 2 0 0 4 1 0 0 5 2 0 zmp zhp zli zpr 8 1 4 2 3 0 6 1 0 2 2 7 3 0 1 2 1 2 6 0 0 0 5 0 0 1 2 0 0 2 5 0 0 1 1 1 1 0 1 1 0 0 0 1 0 0 0 0 0 2 4 0 0 0 2 0 0 0 2 0 0 0 2 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 3 0 1 3 0 0 2 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 9,5% 16,7% 17,2% 4,5% 0,7% 2,1% 0,5% CETEC Curso “Aplicação de Ecomorfologia Fluvial na Validação da Tipologia de Rios” 16-18/06/2010 31 Multimetric Assessment and Assessment Software ASTERICS v.3.0 Results (A) Assessment: Display of scores of the Ecological Quality Class together with the Core Metrics and underlying results. CETEC Curso “Aplicação de Ecomorfologia Fluvial na Validação da Tipologia de Rios” 16-18/06/2010 32 Multimetric Assessment and Assessment Software ASTERICS v.3.0 - Level 1: Ecological quality class (integrated assessment) - Level 2: Quality classes of the single moduls - Level 3: Results of the core metrics - Level 4: Results of all metrics CETEC Curso “Aplicação de Ecomorfologia Fluvial na Validação da Tipologia de Rios” 16-18/06/2010 33 Multimetric Assessment and Assessment Software ASTERICS v.3.0 Export-Function The software has the possibility to export all metric results to Excel CETEC Curso “Aplicação de Ecomorfologia Fluvial na Validação da Tipologia de Rios” 16-18/06/2010 34 Interpretation of assessment results Examples of taxa, ecological information, species traits and the indicative potential as a basis for multimetric assessment systems and the interpretation of results Multimetric Assessment and Assessment Software Interpretation of Assessment Results Mid-sized sand-bottom lowland streams Lachte NI CETEC Curso “Aplicação de Ecomorfologia Fluvial na Validação da Tipologia de Rios” 16-18/06/2010 36 Multimetric Assessment and Assessment Software Interpretation of Assessment Results Scores Lachte Probenahme Staat Fließgewässertyp Taxaliste Stressor Ergebnis Lachte Germany, official system Typ 15: Sand- und lehmgeprägte Tieflandflüsse original General degradation Toleranz Funktionen Funktionen Zusammensetzung Vielfalt, Diversität - Fauna Index D15/17 - Litoral [%] - Pelal [%] - EPT [%] (abundance classes) - # Trichoptera result score (0-1) 0,9 1,154 1 3,84 1 4,63 1 53,52 0,74 8 0,67 quality class high high high high good good CETEC Curso “Aplicação de Ecomorfologia Fluvial na Validação da Tipologia de Rios” 16-18/06/2010 37 Multimetric Assessment and Assessment Software Interpretation of Assessment Results Results Lachte Metric Value Abundance [Ind./m²] 561.6 Number of Taxa 35 German Saprobic Index 1.85 Fauna Index Typ 15/17 1.15 Diversity (Shannon-Wiener-Index) 2.32 EPT [%] (abundance classes) 53.5 CETEC Curso “Aplicação de Ecomorfologia Fluvial na Validação da Tipologia de Rios” 16-18/06/2010 38 Multimetric Assessment and Assessment Software Taxa-Group Taxalist Lachte Interpretation of Assessment Results Taxon name Ab. % Bivalvia Pisidium sp. 0,8 0,1 Bivalvia Sphaerium sp. 0,8 0,1 Oligochaeta Oligochaeta Gen. sp. 2,4 0,4 Hirudinea Erpobdellidae Gen. sp. 4,8 0,9 Crustacea Asellus aquaticus 0,8 0,1 Crustacea Gammarus pulex 1,6 0,3 Crustacea Gammarus sp. 20,8 3,7 Ephemeroptera Baetidae Gen. sp. Ephemeroptera Serratella ignita Ephemeroptera Ephemeroptera 6,4 1,1 103,2 18,4 Heptageniidae Gen. sp. 8,8 1,6 Electrogena sp. 2,4 0,4 Ephemeroptera Heptagenia sp. 6,4 1,1 Odonata Gomphus vulgatissimus 0,8 0,1 Odonata Ophiogomphus cecilia 5,6 1,0 Plecoptera Leuctra sp. 78,4 14,0 Trichoptera Lype reducta 1,6 0,3 Trichoptera Hydropsyche pellucidula 18,4 3,3 Trichoptera Hydropsyche sp. 90,4 16,1 Trichoptera Halesus radiatus 2,4 0,4 Trichoptera Halesus digitatus/tesselatus Trichoptera Brachycentrus subnubilus Trichoptera Trichoptera 0,8 0,1 147,2 26,2 Sericostoma flavicorne/personatum 0,8 0,1 Lepidostoma hirtum 1,6 0,3 Trichoptera Mystacides azurea 0,8 0,1 Coleoptera Limnius volckmari Lv. 8,8 1,6 Coleoptera Limnius volckmari Ad. 0,8 0,1 Coleoptera Oulimnius tuberculatus Ad. 4 0,7 Coleoptera Hydraena sp. Ad. 1,6 0,3 Coleoptera Oreodytes sanmarkii Ad. 0,8 0,1 Diptera Atherix ibis 9,6 1,7 Diptera Atrichops crassipes 0,8 0,1 Diptera Limoniidae Gen. sp. 0,8 0,1 Diptera Chironomini Gen. sp. 10,4 1,9 Diptera Tanytarsini Gen. sp. 3,2 0,6 Diptera Chironomidae Gen. sp. 12,8 2,3 CETEC Curso “Aplicação de Ecomorfologia Fluvial na Validação da Tipologia de Rios” 16-18/06/2010 39 Multimetric Assessment and Assessment Software Interpretation of Assessment Results Stever near Füchtelner Mühle (NRW) CETEC Curso “Aplicação de Ecomorfologia Fluvial na Validação da Tipologia de Rios” 16-18/06/2010 40 Multimetric Assessment and Assessment Software Level 1: Interpretation of Assessment Results Ecological quality class poor Level 2: Results of the different modules - Saprobic index good SI = 2.29 - General degradation poor Score = 0.23 Thus, the reason for the poor status is the general/hydromorphological degradation. To rise the ecological status the hydromorphology of the site has to be improved. CETEC Curso “Aplicação de Ecomorfologia Fluvial na Validação da Tipologia de Rios” 16-18/06/2010 41 Multimetric Assessment and Assessment Software Level 3: Interpretation of Assessment Results Scores of the core metrics • German Fauna Index 0,09 bad rare and type specific species are missing • # Trichoptera 0,13 bad • EPT-Taxa [%] 0,25 poor poor habitat diversity, e.g. organic substrates are absent (woody debris, CPOM) • Litoral [%] 0,24 poor • Pelal [%] 0,87 reference Low species-richness and shifted abundances Disturbance of natural flow regime (stagnant situations, absence of shading and high amounts of macrophytes) Summary: The single results imply that the site is hydromorphologically extemely impaired. The effects on the invertebrate community are obvious (Fauna Index, number of Trichoptera taxa, % Littoral preferences). CETEC Curso “Aplicação de Ecomorfologia Fluvial na Validação da Tipologia de Rios” 16-18/06/2010 42 Multimetric Assessment and Assessment Software Taxa-Group Taxalist Stever Interpretation of Assessment Results Taxon name Gastropoda Bithynia tentaculata Gastropoda Ab. % 9,6 0,3 Potamopyrgus antipodarum 19,2 0,6 Gastropoda Valvata sp. 33,6 1,0 Bivalvia Dreissena polymorpha 19,2 0,6 Bivalvia Pisidium sp. 4,8 0,1 Oligochaeta Naididae/Tubificidae Gen. sp. 4,8 0,1 Hirudinea Erpobdellidae Gen. sp. 4,8 0,1 Hirudinea Glossiphonia complanata 4,8 0,1 Hirudinea Helobdella stagnalis 28,8 0,9 Crustacea Asellus aquaticus 216 6,4 Crustacea Gammarus roeselii 1526,4 45,2 Crustacea Gammarus sp. 657,6 19,5 Ephemeroptera Baetidae Gen. sp. 0,8 0,0 Ephemeroptera Caenis horaria/robusta 9,6 0,3 Ephemeroptera Caenis luctuosa/macrura 38,4 1,1 Ephemeroptera Caenis sp. 57,6 1,7 Ephemeroptera Cloeon sp. 9,6 0,3 Heteroptera Corixidae Gen. sp. 4,8 0,1 Coleoptera Haliplus sp. Ad. 4,8 0,1 Coleoptera Oulimnius sp. Lv. 33,6 1,0 Trichoptera Cyrnus trimaculatus 9,6 0,3 Trichoptera Hydroptila sp. 28,8 0,9 Diptera Chironomidae Gen. sp. 134,4 4,0 Diptera Chironomini Gen. sp. 480 14,2 Diptera Tanytarsini Gen. sp. 33,6 1,0 CETEC Curso “Aplicação de Ecomorfologia Fluvial na Validação da Tipologia de Rios” 16-18/06/2010 43 Multimetric Assessment and Assessment Software Ebene Level 3:3: Interpretation of Assessment Results Ergebnisse dercore Coremetrics Metrics Scores of the • German Fauna Index 0,09 bad • # Trichoptera 0,13 bad • EPT-Taxa [%] 0,25 poor • Litoral [%] 0,24 poor • Pelal [%] 0,87 reference CETEC Curso “Aplicação de Ecomorfologia Fluvial na Validação da Tipologia de Rios” 16-18/06/2010 44 Multimetric Assessment and Assessment Software Standardized sampling method: Multi-Habitat-Sampling (AQEM method = EUROPEAN „CEN“ Standard since 2010) 25 cm 25 cm Netzmaschenweite: 0,5 mm Sand (55 % = 11 Teilproben) Fallaub (15 % = 3 Teilproben) Steine (<5% = 0 Teilproben) Holz (5 % = 1 Teilprobe) Kies (25 % = 5 Teilproben) Teilprobe CETEC Curso “Aplicação de Ecomorfologia Fluvial na Validação da Tipologia de Rios” 16-18/06/2010 Multimetric Assessment and Assessment Software Standardized treatment of the samples (sieving and sub-sampling CETEC Curso “Aplicação de Ecomorfologia Fluvial na Validação da Tipologia de Rios” 16-18/06/2010 Autecology on species level Ecological indicators Basis for assessment