Cleiton H. Sequeira USDA NRCS-National Soil Survey Center 100 Centennial Mall North, Rm 416, Lincoln, NE 68508-3866 Mobile: 540-553-4410 [email protected] Education Doctor of Philosophy, Crop and Soil Environmental Sciences, February 2011 Virginia Polytechnic Institute and State University (Virginia Tech), Blacksburg, VA Dissertation: Soil organic matter dynamics in cropping systems of Virginia’s Valley region GPA: 3.78/4.0 Master of Science, Soil and Plant Nutrition, October 2007 Universidade Federal de Viçosa, Viçosa, MG, Brazil Thesis: Availability of micronutrients in soils and its relationship with foliar contents in young Eucalyptus stands GPA: 3.0/3.0 Bachelor of Science, Agronomy, July 2004 Universidade Federal de Viçosa, Viçosa, MG, Brazil Research Experience Rapid Assessment of U.S. Soil Carbon for Climate Change and Conservation Planning Project – University of Nebraska-Lincoln in partnership with USDA NRCS-National Soil Survey Center (March 2011 – Present) Development of Visible Near Infrared (VNIR) models to predict soil properties such as organic carbon, cation exchange capacity, and clay content Evaluation of soil property and landscape effects on soil carbon dynamics Evaluation of ecosystem properties and agricultural management effects on carbon dynamics Assist with general project oversight, database design, troubleshoot, quality assurance, quality control, and data management Provide training, reporting, communication, and publicity of the project Produce a nationwide inventory of soil carbon stocks in the U.S. Nitrate and Phosphate Leaching Losses in Agronomic Crop Production in the Virginia Coastal Plain – Virginia Tech (January 2008 – December 2010) Collection, in monthly basis, of leached water from 24 passive capillary lysimeters located on two commercial farms Analysis of the water samples for nitrate and phosphate Collection of yield samples for wheat, soybean, and corn in the experiment sites Maintenance of the lysimeters Data analysis to compare amounts of nitrate and phosphate leached through soil profile due to tillage and crop rotation treatments Influence of Cropping Systems on Carbon Sequestration in Virginia Soils – Virginia Tech (January 2008 – December 2010) Preparation of the research project Collection of soil samples from 19 sites around the state of Virginia to determine total organic carbon and nitrogen contents Conducted laboratory analysis and supervision of one undergrad student Data analyses to compare the effect of tillage and crop rotation on soil total organic carbon and nitrogen contents at 0-5, 5-15, 15-30, and 30-60 cm depths for the 19 sites 1 Role of Soil Organic Matter as Source of Nitrogen in Commercial Corn Production – Virginia Tech (January 2008 – December 2010) Preparation of the research project Collection of soil samples from 11 sites in Virginia without nitrogen applied and determination of their nitrogen contents by different methods Collection of whole-corn plant samples from the 11 sites at R4-R5 growth stages and determination of nitrogen contents Conducted laboratory analysis and supervision of two undergrad students Fitting of simple and multiple linear regression models to predict residual nitrogen availability to corn plants Actives Fractions of Soil Organic Matter as Indices of Soil Change due to Management Practices – Virginia Tech (January 2008 – December 2010) Preparation of the research project Collection of soil samples from an agronomic experiment located in the Valley region of Virginia Conducted analyses to determine carbon and nitrogen contents into different fractions of soil organic matter and supervision of one undergrad student Data analysis to determine the most sensitive organic fraction to changes in soil due to crop rotation, tillage, and cover crop management Research Assistant – Virginia Tech (May 2008 – August 2010). Under supervision of Dr. Marcus M. Alley Supported field experiments: layout, planting, treatments application, and harvesting Literature review, data analysis, and preparation of reports and posters Availability and Supply of Micronutrients in Eucalyptus Stands – Universidade Federal de Viçosa (July 2005 – October 2007) Preparation of the research project Collection of soil and eucalypt leaves samples from the 27 sites throughout the state of Minas Gerais, Brazil Conducted analyses to determine copper, zinc, manganese, and iron contents in soil and plant samples Supervision of one undergrad student Data analysis to determine the best extractant of micronutrients to eucalypt under supervision of Dr. Júlio C.L. Neves Effects of Weeds Root Exudation and Drift of Glyphosate on Eucalyptus Growth – Universidade Federal de Viçosa (September 2004 – January 2005) Support in installing and conducting greenhouse experiments Support in laboratory analyses Support in the publication of scientific papers (literature review and writing) Undergrad Research – Universidade Federal de Viçosa (June 2002 – June 2003). Under supervision of Dr. Nairam F. Barros Conducted a greenhouse experiment designed to evaluated the agronomic efficiency and impact on soil of five industrial sludge Determination of heavy metals in both soil and corn samples Data analysis under supervision of Dr. Júlio C.L. Neves 2 Teaching Experience Teaching Assistant – Virginia Tech (fall 2010). Under supervision of Dr. Steven Hodges Direction of lab sections of the senior level Soil Fertility and Management class Worked one-to-one with students on problems and concepts Replaced the main instructor in one lecture so far Preparation of exams Grading quizzes and exams Teaching Assistant – Virginia Tech (spring 2008). Under supervision of Dr. James R. McKenna. Management of the multimedia lab of the Agronomic Crops, and World Crops and Cropping Systems classes Worked one-to-one with students on problems and concepts Grading quizzes and exams Internships Votorantim Celulose e Papel (VCP) – Votorantim Cellulose and Paper, Luíz Antônio, SP, Brazil (May 2002 – June 2002). Conducted sampling, measurements, and maintenances in the irrigated forest project Maintenance of the clone propagation center Instituto Agronômico de Campinas (IAC) – Sugarcane Advanced Technology Research Center, Ribeirao Preto, SP, Brazil (September 2002 – November 2002). Supported the planting and harvesting breeding plots Evaluation of yield and disease trials Supported in answering questions to the public about specific IAC’s varieties native fluent moderate Languages Portuguese English Spanish Computer Skills Microsoft Office 2007 (Word, Excel, Access, PowerPoint) R, SAS, JMP, R, Minitab, and STATISTICA Geographic Information System (GIS) Awards/ Affiliations Gama Sigma Delta – The Honor Society of Agriculture, 2011- present Samuel Obenshain Graduate Scholarship Award 2009 Master of Science CAPES fellowship research, 2005 - 2007 American Society of Agronomy, 2008 - present Crop Science Society of Agronomy, 2008 - present Soil Science Society of America, 2008 - present Publications Spargo, J., M. Cavigalli, J. Maul, C.H. Sequeira, M.M. Alley, and R. Follet. 2012. Changes in soil organic carbon and nitrogen fractions with duration of no-tillage management. Soil Science Society of America Journal. (Under review) Sequeira, C.H. and M.M. Alley. 2011. On-farm effects of tillage on soil organic matter levels in the Shenandoah Valley Region of Virginia. Agronomy Journal. (Under review) 3 Sequeira, C.H. and M.M. Alley. 2011. Soil organic matter fractions as indices of soil quality changes. Soil Science Society of America Journal, 75:1766-1773. Sequeira, C.H., N.F. Barros, J.C.L. Neves, R.F. Novais, I.R. Silva, and M.M. Alley. 2011. Micronutrient soil-test levels and Eucalyptus foliar contents. Communications in Soil Science and Plant Analysis, 42:475–488. Sequeira, C.H., M.M. Alley, and B.P. Jones. 2011. Evaluation of potentially labile soil organic carbon and nitrogen fractionation procedures. Soil Biology & Biochemistry, 43: 438–444. Santos, L.D.T., C.H. Sequeira, N.F. Barros, F.A. Ferreira, and L.R. Ferreira. 2007. Growth and concentration of nutrients in the aerial tissue of eucalypt under glyphosate drift effect. Revista CERNE, 13:347-352. Sequeira, C.H., M.S. Barbosa, H.E.P. Martinez, P.R.G. Pereira, and P.C.R. Pereira. 2006. Fontes. The effect of foliar applications of calcium and titanium tetrachloride upon the occurrence of “Tip burn” in lettuce plants. BioScience Journal, 22:7-16. Santos, L.D.T., F.A. Ferreira, N.F. Barros, C.H. Sequeira, I.C. Santos, and A.F.L. Machado. 2005. Root exudation of glyphosate by Brachiaria decumbens and its effects on eucalypt plants and microbial soil respiration. Revista Planta Daninha, 23:143-152. Abstracts Sequeira, C.H., E. Benham, R. Feguson, D. Harms, S. Kenneth, Z. Libohova, S. Monteith, C. Seybold, L. West, and S. Wills. 2011. U.S. soil carbon assessment with VNIR diffuse reflectance spectroscopy. In 2011 ASA-CSSA-SSSA International Annual Meetings. San Antonio, TX. Wills, S., L. West, and C.H. Sequeira. 2011. Sample design for national carbon stock assessment. In 2011 ASA-CSSA-SSSA International Annual Meetings. San Antonio, TX. Sequeira, C.H. and M.M. Alley. 2010. Labile fractions of soil organic matter as indices of soil change associated with management practices. In 2010 ASA-CSSASSSA International Annual Meetings abstracts. Long Beach, CA. Sequeira, C.H., M.M. Alley, and B.P. Jones. 2009. Sodium iodide and sodium polytungstate solutions for recovering free light fraction soil organic matter. In 2009 ASA-CSSA-SSSA International Annual Meetings abstracts. Pittsburgh, PA. Spargo, J., M.M. Alley, C.H. Sequeira, S.B. Mirsky, and J.V. Wallace. 2009. Influence of tillage on nitrate leaching losses in the Virginia Coastal Plain. In 2009 ASA-CSSA-SSSA Inernational Anual Meetings abstracts. Pittsburgh, PA. Sequeira, C.H., J. Spargo, and M.M. Alley. 2009. Nitrate leaching in n-tillage versus tilled fields. In 2009 Virginia Water Research Conference. Richmond, VA. Oliveira, F.C.C., C.H. Sequeira, N.F. Silva, and N.F. Barros. 2009. Disponibilidade de boro em solos do estado de Minas Gerais e seus teores foliares em plantas jovens de eucalipto (Availability of boron in soils of the state of Minas Gerais and its foliar 4 contents in young eucalypt plants). In XXXII Congresso Brasileiro de Ciência do Solo. Fortaleza, CE. Sequeira, C.H., N.F. Barros, and M.M. Alley. 2008. Availability of micronutrients in soils and its correlation with foliar contents in young plantations of Eucalyptus. In 2008 ASA-CSSA-SSSA International Annual Meetings abstracts. Houston, TX. Barbosa, M.S., C.H. Sequeira, L.M. Alvarenga, and H.P. Martinez. 2005. Efeito da aplicação foliar de cálcio e tatracloreto de titânio sobre a ocorrência de “Tip Burn” em plantas de alface (The effect of foliar applications of calcium and titanium tetrachloride upon the occurrence of “Tip burn” in lettuce plants). In VI Simpósio Latino Americano de Ciência de Alimentos. Campinas, SP. Barbosa, M.S., J.G. Barbosa, J.A.S. Grossi, S.A. Tinoco, M.A. Muniz, C.H. Sequeira, A.F. Santos, and M. Raimundo. 2004. Produção e qualidade de crisântemo de vaso em função da aplicação parcelada de fósforo (Yield and quality of chrysanthemun in pots due to splited application of phosphorus). In 2004 Fórum Latino-Americano sobre Plantas Ornamentais. Nova Petrópolis, RS. Santos, L.D.T., F.A. Ferreira, N.F. Barros, L.R. Ferreira, C.H. Sequeira, and A.F.L. Machado. 2004. Simulação de deriva do glyphosate em eucalipto (Simulation of glyphosate drift in eucalyptus). In XXIV Congresso Brasileiro da Ciência das Plantas Daninhas. São Pedro, SP. Sequeira C.H., L.D.T. Santos, N.F. Barros, F.A. Ferreira, and L.R. Ferreira. 2004. Crescimento e concentração de nutrientes na parte aérea de eucalipto sob efeito da deriva do glyphosate (Growth and concentration of nutrients in the aerial part of eucalypt under the effect of glyphosate drift). In FERTBIO 2004. Lages, SC. Santos, L.D.T., R.M.S.A. Meira, F.A. Ferreira, C.H. Sequeira, N.F. Barros, and L.R. Ferreira. 2004. Efeito de subdoses crescentes de glyphosate na morfoanatomia de eucalipto (Effect of crescent rates of glyphosate on the morphoanatomy of eucalypt). In LV Congresso Nacional de Botânica. Viçosa, MG. Santos, L.D.T., R.M.S.A. Meira, F.A. Ferreira, C.H. Sequeira, N.F. Barros, and L.R. Ferreira. 2004. Anatomia foliar do eucalipto sob efeito de subdoses de glyphosate simulando deriva (Foliar anatomy of eucalypt under effect of different rates glyphosate drift). In LV Congresso Nacional de Botânica. Viçosa, MG. Santos, L.D.T., R.A.S. Tiburcio, F.A. Ferreira, J.B. Santos, C.H. Sequeira, and A.F. Machado. 2004. Efeito do glyphosate na respiração microbiana de dois solos cultivados com eucalipto (Effetc of glyphosate on micobial respiration of two soils cultivated with eucalypt). In III Simpósio de Meio Ambiente. Viçosa, MG. Sequeira, C.H., N.F. Barros, J.C.L. Neves, F.P. Leite, J.L. Teixeira, and R.F. Novais. 2003. Eficiência agronômica e efeito no solo de resíduos da indústria de celulose. (Agronomic efficiency of cellulose industry residues and their effect on soil). In XXIX Congresso Brasileiro de Ciência do Solo. Ribeirão Preto, SP. 5 Oral Presentations U.S. soil carbon assessment with VNIR diffuse reflectance spectroscopy. In 2011 ASA-CSSA-SSSA International Annual Meetings. San Antonio, TX. Labile fractions of soil organic matter as indices of soil change associated with management practices. In 2010 ASA-CSSA-SSSA International Annual Meetings. Long Beach, CA. Sodium iodide and sodium polytungstate solutions for recovering free light fraction soil organic matter. In 2009 ASA-CSSA-SSSA International Annual Meetings. Pittsburgh, PA. Nitrate leaching in no-tillage versus tilled fields. In 2009 Virginia Water Research Conference. Richmond, VA. 6