th
XI Reunião Regional Nordeste da SBBq
4 International Symposium in Biochemistry of Macromolecules and Biotechnology
Recife – PE, 5 a 7 de dezembro de 2012
Preliminary Investigation of the Brain Acetylcholinesterase from
Serrasaulmus sp as a Biomarker on the Itaparica Reservoir – PE/BA
Santos, F.L.B1,2; Silva, J. M.¹; Alves, R.M.¹; Leoncini, G.O.¹; Silva, J. G¹; Machado,
S. S.¹; Abreu, F.C.¹
¹Institute of Chemistry and Biotechnology, Federal University of Alagoas, Maceió
– AL, Brazil; 2Department of Education, Campus VIII, University of the State of
Bahia, Paulo Afonso-BA, Brazil.
Although Brazil has started to enforce environmental regulations to restrict the use
of agrochemicals, it remains very high. Brazil is on the list of country showing the
highest consumption in the world and the use of agrochemicals has increased
over the last few decades. The water reservoir of the Itaparica hydroelectric is
located between the Brazilian northeast states of Pernambuco and Bahia,
submedium São Francisco river. There are several small agricultural settlements
around the reservoir using agrochemicals. Aiming to investigate the environmental
impact of the agrochemicals in the reservoir this work has been doing preliminary
investigation of the acetylcholinesterase-AChE on the brain of three fish species
present in the reservoir. The fish species will be monitored in two established
points of the lake during one year. Eserine (physostigmine) under concentration
range from 0.015 to 2.5µM was used to investigate the inhibitory effect of
carbamate pesticides. One the species, Pirambeba (Serrasalmus sp) is a native
fish of the Sao Francisco river and the
AChE specific activity on the three
sampled times (March, May and July 2012) showed the following results 0.394 e
0.132; 0.353 e 049; 0.331 e 0.539µmol.min -1.mg protein-1 . The inhibitory studies
indicated that Serrasalmus sp has a moderate sensitivity to the carbamate eserine
(IC50= 0.818 µM with a 95% confidence interval).
Key words: acetylcholinesterase, agrochemicals, fish