rd
23 Congress of the International Union for Biochemistry and Molecular Biology
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44 Annual Meeting of the Brazilian Society for Biochemistry and Molecular Biology
th
th
Foz do Iguaçu, PR, Brazil, August 24 to 28 , 2015
CHARACTERIZATION OF TRANSPOSABLE ELEMENTS FROM A
DROSOPHILA PARASITOID WASP BELONGING TO BRACONIDAE FAMILY
Silva, A.F.1; Carvalho, E.L.1,2; Macedo, P.E.1,2; Tapia, J.S.1,2; Rangel, D.L.1;
Ferreira, M.F.1; Machado, L.C.1; Silva, L.F.D.1; Miranda, T.O.1; Dezordi, F.Z.1;
Dotto, D.R.1; Araujo, T.F.1; Loreto, E.L.S.3; Pinto, P.M.1,2; Wallau, G.L.1,2
1
Laboratório de proteômica aplicada, Universidade Federal do Pampa, São
Gabriel, Rio Grande do Sul, Brasil; 2 Programa de pós-graduação em Ciências
Biológicas, Universidade Federal do Pampa, São Gabriel, Rio Grande do Sul,
Brasil; 3 Departamento de Bioquímica e Biologia Molecular, Universidade Federal
de Santa Maria, Santa Maria, Rio Grande do Sul, Brasil.
Transposable elements (TEs) are mobile genetic elements, presents in
most of the studied genomes to date. These elements are capable of mobilization
inside host genomes. They are grouped in two classes. The class I encompasses
the retrotransposons that use RNA intermediates to its mobilization, while the
class II use DNA intermediates. Currently, TEs have been better characterized
due the new sequencing techniques and genomic analysis. Characterization of
these elements becomes important for better understanding the relation among
the proportion, the presence of the determinate element and its function inside
each genome. In the present work, we aimed to characterize the transposable
elements presents in genome of a parasitoid wasp belonging to Braconidae family
that had its draft genome recently sequenced. After obtained the sequence of
wasp genome, we perform bioinformatics analysis utilizing tools such as:
RepeatExplorer; CENSOR; ORF Finder; Basic local Alignment Search Tool;
MAFTT and PHYML. After RepeatExplorer sequence analysis, was possible find
several TEs within the studied genome. We obtained a total of 73 clusters where
the elements of the class I represented a total of 1,795 %, while the elements of
the class II represented 3,161 % of the genome. The elements most represented
were the Mavericks-Polintons, encompassing 2,108% of the genome. Those TEs
showed high similarity with Mavericks-Polintons of the Nasonia vitripennis in the
Censor analysis. Using the integrase of this elements was possible reconstructed
a phylogenetic tree to investigate the evolutionary relationship this elements
among other well-characterized Mavericks-Polintons from different species. Based
on those analysis it suggests that the elements of the class II are the most
abundant in this genome. The Mavericks-Polintons are the most represented
elements and presented an independent evolutionary pattern, due to the presence
of the two different integrase groups represented in the phylogenetic tree.
We thank to CNPq, CAPES and Fapergs
Key words: Mobile genetic elements, Bioinformatics, Parasitoid wasp
Brazilian Society for Biochemistry and
Molecular Biology (SBBq)