Departamento de Engenharia Biológica Departamento de Engenharia Biológica Universidade do Minho 4710-057 Braga - PORTUGAL Tel: (+351) 253 604 400 Fax: (+351) 253 678 986 www.deb.uminho.pt Universidade do Minho SCHOOL OF ENGINEERING UNIVERSITY OF MINHO December 2004 [6th edition] Departamento de Engenharia Biológica Universidade do Minho Campus de Gualtar 4710-057 Braga PORTUGAL Tel: (351) 253 604 400 Fax: (351) 253 678 986 www.deb.uminho.pt INDEX 1. INTRODUCTION .................................................................................................................................................3 MANAGEMENT BOARDS....................................................................................................................................3 2. ACADEMIC AND NON ACADEMIC STAFF...............................................................................................................3 3. LABORATORIES................................................................................................................................................25 4. EDUCATION.....................................................................................................................................................27 BIOLOGICAL ENGINEERING COURSE (GRADUATE DEGREE) ..............................................................................................27 PROFESSIONAL SITUATION OF THE GRADUATES IN BIOLOGICAL ENGINEERING .......................................................................28 INTERNATIONAL STUDENTS EXCHANGE PROGRAMMES ...................................................................................................28 CURRICULUM OF BIOLOGICAL ENGINEERING ..............................................................................................................29 CURRICULUM OF BIOLOGICAL ENGINEERING - TRACK OF POLLUTION CONTROL .......................................................................30 CURRICULUM OF BIOLOGICAL ENGINEERING - TRACK OF FOOD AND CHEMICAL TECHNOLOGY.....................................................30 BIOMEDICAL ENGINEERING COURSE (GRADUATE DEGREE) .............................................................................................31 CURRICULUM OF BIOMEDICAL ENGINEERING ...............................................................................................................32 CURRICULUM OF BIOMEDICAL ENGINEERING - TRACK OF CLINICAL ENGINEERING ...................................................................33 M.SC. IN ENVIRONMENTAL TECHNOLOGY ..................................................................................................................34 M.SC. IN ENVIRONMENTAL MANAGMENT ..................................................................................................................35 M.SC. IN BIOTECHNOLOGY - BIOPROCESS ENGINEERING ...............................................................................................36 5. RESEARCH ......................................................................................................................................................37 NON- PERMANENT STAFF .....................................................................................................................................37 RESEARCH ON GOING PROJECTS ............................................................................................................................39 PH.D. AND M.SC. THESES ...................................................................................................................................41 6. LIST OF PUBLICATIONS (SINCE 2002) ...............................................................................................................43 BOOKS ...........................................................................................................................................................43 BOOK CHAPTERS ...............................................................................................................................................43 PEER-REVIEWED PAPERS .......................................................................................................................................46 7. THE "MICOTECA DA UNIVERSIDADE DO MINHO"................................................................................................53 2 INTRODUCTION 1. Introduction The Department of Biological Engineering (DEB) is a permanent structure of the School of Engineering of the University of Minho, responsible for the production and transmission of knowledge in the scientific fields of Biological Engineering, Chemical Engineering and Biomedical Engineering. This Department hosts the research facilities of the Centre of Biological Engineering (CEB) MANAGEMENT BOARDS CEB • General Assembly • Direction Board • Head of CEB: Prof. M. Mota DEB • • • • • Department Assembly Department Council Scientific Board Consulting Board Head of DEB: Prof. J.A. Teixeira 2. Academic and Non Academic Staff Faculty Members Luís de Jesus Santos Soares João de Deus Rogado Salvador Pinheiro Manuel José Magalhães Gomes Mota José António Couto Teixeira Rosário V. J. Tavares de Oliveira Nelson Manuel Viana da Silva Lima Maria Teresa Campos Tavares Eugénio Manuel F. Campos Ferreira Maria João Lopes da Costa Vieira Franscisco Miguel Portela da Gama António José Guerreiro Brito Armando Albino Dias Venâncio Maria Madalena dos Santos Alves Isabel Maria Pires Belo António A. Oliveira Soares Vicente Joana C. Valente Rodrigues Azeredo José Maria Marques Oliveira Regina Maria B. Nogueira João Monteiro Peixoto Lucília M. A. Ribeiro Domingues Maria Olívia B. Pereira Administrative and Technical Staff Maria Madalena Costa Vieira Maria dos Anjos V. Cardoso Costa Manuel Araújo Gomes dos Santos Maria Helena Rio Madeira Silva Isabel Maria da Silva Soares Maria de Fátima Veríssimo Jacinto Maria Adelaide Novais Francisco Augusta Sameiro Ferreira Martins Maria da Glória da Silva Cruz Martins 3 DEPARTMENT OF BIOLOGICAL ENGINEERING FACULTY MEMBERS Luís de Jesus Santos Soares Full Professor Education: • • • Positions: • • Ph.D. in Chemical Engineering, University of Birmingham, 1972 Master in Chemical Engineering, University of Birmingham, 1970 Graduation in Chemical Engineering, FEUP, 1965 Full Professor, UM President of the Politechnic Institute of Porto Teaching Areas: • n/a Research Activities: • n/a Selected publications: Bernardo-Gil MG, Soares L. Liquid-liquid equilibria of systems containing perfluoromethylcyclohexane. Fluid Phase Equilibria 61, 121-124, 1990. • Bernardo-Gil MG, Soares L. Liquid-liquid equilibria for the system perfluorodecalin/1-heptene/nheptane/n-hexane. Journal Chemical Engineering Data 34, 103-107, 1989. • Bernardo-Gil MG, Soares L. Mutual binary solubilities: perfluoromethyl-cyclohexane/hydrocarbons. Fluid Phase Equilibria 41, 205-208, 1988. • Bernardo-Gil MG, Soares L. Mutual binary solubilities: perfluoro-decalin/hydrocarbons. Journal Chemical Engineering Data 32, 327-330, 1987. • Bernardo-Gil MG, Soares L. Perfluorodecalin/hydrocarbon systems: prediction and Correlation of Liquid-liquid Equilibrium Data. Fluid Phase Equilibria 25, 291-296, 1986. • 4 DEPARTMENT OF BIOLOGICAL ENGINEERING FACULTY MEMBERS João de Deus Rogado Salvador Pinheiro Full Professor Education: • • Positions: PhD in Chemical Engineering (Birmingham, UK, 1976) Graduation in Chemical Engineering (IST, Lisbon) • • • • • • • • • • • • • • Full Professor, UM Rector of Universidade Moderna (2001-2002) European Commissioner (1993-1999) Ministry for Foreign Affairs (1987-1992) Ministry for Education (1985-1987) Rector of Universidade do Minho (1984-1985) Vice-Rector of Universidade do Minho (1981, 1983-1985) "Secretário de Estado da Educação e Administração Escolar" (1982-1983) Vice-President of JNICT (1981) Associate Professor, UM, 1989– Assistant Professor, UM, 1987-1989 Invited Assistant, UM, 1976-1987 Teaching Assistant, University of Lourenço Marques, 1971-1972 Secondary School Teacher, 1970-1971 • • Seminars Strategy of Process Engineering • n/a Main Teaching Areas: Research Activities: Selected publications: Pinheiro MM, Melo L, Pinheiro JD. Surface phenomena and hydrodynamic effects on the deposition of Pseudomonas fluorescens. Canadian Journal Chemical Engeneering 66, 66-70, 1988. • Melo L, Pinheiro JD. Particle transport in fouling caused by kaolin-water suspensions on copper tubes. Canadian Journal Chemical Engineering 66, 36-41, 1988. • Melo L, Pinheiro JD. Fouling by aqueous suspensions of kaolin and magnetite - hydrodynamic and surface phenomena effects. In Fouling Science and Technology, L Melo, TR.Bott, C Bernardo (Eds), Kluwer: Dordrecht, 173-189, 1988. • Melo L, Pinheiro JD. Particulate fouling in boiling water systems. In Two-Phase Flow Heat Exchangers: Thermal-Hydraulic Fundamentals and Design, S Kakaç, A Bergles, E O Fernandes, (Eds), Kluwer, Dordrecht, 461-469, 1988. • 5 DEPARTMENT OF BIOLOGICAL ENGINEERING FACULTY MEMBERS Manuel José Magalhães Gomes Mota Full Professor Education: • • • • Positions: • • • • • • • Ph.D. in Biochemical Engineering, INSA, Toulouse, 1985 Master in Microbiology, INSA, Toulouse, 1982 Service in the Army 1973-1976 Graduation in Chemical Engineering, FEUP, 1972 Vice-rector for Research and external Affairs, UM, 2002Full Professor, UM, 1994Associate Professor, UM, 1991-1994 Associate Professor, FEUP, 1990-1991 Assistant Professor, FEUP, 1985-1990 Teaching Assistant, Faculty of Engineering, 1978-1981 Teaching Assistant, Polytechnic Institute of Oporto, 1976-1978 Main Teaching Areas: • Introduction to Biomedical Engineering Research Activities: • • • • • Population dynamics in aerobic and anaerobic processes Porous media properties Hyperbaric bioreactors Multiphase bioreactors Biodegradability of implants Selected publications: Araújo MA, Cunha AM, Mota M. Enzymatic degradation of starch based thermoplastic compounds used in protheses: identification of the degradation products in solution. Biomaterials 25, 2687-2693, 2004. • Mota M, Teixeira J.A. Keating, JB, Yelshin A. Changes in diffusion through the brain extracellular space. Biotechnology and Applied Biochemistry 39, 223-232, 2004. • Dias R, Teixeira JA, Mota M, Yelshin A. Preparation of controlled particulate mixtures with glass beads of different sizes. Separation and Purification Technology 37, 69-80, 2004. • Belo I, Pinheiro R, Mota M. Fed-batch cultivation of Saccharomyces cerevisiae in a hyperbaric bioreactor. Biotechnology Progress 19, 665-671, 2003. • Cabral JMS, Mota M, Tramper J (Eds) Multiphase Bioreactor Design, Taylor & Francis, London 2001 (ISBN: 0415272092). • 6 DEPARTMENT OF BIOLOGICAL ENGINEERING FACULTY MEMBERS José António Couto Teixeira Full Professor Education: • • Positions: • • • • • Ph.D. in Chemical Engineering, FEUP, 1988 Graduation in Chemical Engineering, FEUP, 1980 Full Professor, UM, 2001Associate Professor, UM, 1993-2001 Associate Professor, FEUP, 1993 Assistant Professor, FEUP, 1988-1993 Teaching Assistant, FEUP, 1980-1988 Main Teaching Areas: • • Biological Reactors Food Biotechnology Research Activities: • • • • • Biological Multiphase Reactors Bioreactors Hydrodynamics and Mass Transfer Downstream Processing Food Technology Heavy Metal Removal Selected publications: Brányik T, Vicente AA, Cruz JMM, Teixeira JA. Continuous Primary Fermentation of Beer with Yeast Immobilized on Spent Grains – The Effect of Operational Conditions. Journal of the American Society of Brewing Chemists 62, 29-34, 2004. • Klein J, Vicente AA, Teixeira JA. Hydrodynamic considerations on optimal design of a three-phase airlift bioreactor with high solids loading. Journal of Chemical Technology and Biotechnology 78, 935-944, 2003. • Xu X, Madeira PP, Teixeira JA, Macedo EA. A new modified Wilson Equation for the calculation of vapor-liquid equilibrium of aqueous polymer solutions. Fluid Phase Equilibria 213 (1-2), 53-63, 2003. • Castro I, Gonçalves O, Teixeira JT, Vicente AA. Comparative Study of Selva and Camarosa Strawberries for the Commercial Market. Journal of Food Science 67:6, 2132-2137, 2002. • Domingues L, Lima N, Teixeira JA. Alcohol production from cheese whey permeate using genetic modified flocculating yeast cells. Biotechnology and Bioengineering 72, 507-514, 2001. • 7 DEPARTMENT OF BIOLOGICAL ENGINEERING FACULTY MEMBERS Rosário V. J. Tavares de Oliveira Full Professor Education: • • Positions: • • • • • Ph.D. in Engineering Science, UM, 1991 Graduation in Chemical Engineering, University of Luanda, 1975 Full Professor, UM, 2003 Associate Professor, UM, 1996 -2003 Assistant Professor, UM, 1991-1996 Invited Assistant Professor, UM, 1982-1991 High School Teacher of Physics and Chemistry, 1976-1982 Main Teaching Areas: • • • • • Instrumental Methods of Analysis Separation Processes in Biotechnology Solid Waste Treatment Elements of Biotechnology Interfacial Phenomena Research Activities: • • • Microbial adhesion Biofilm formation, composition and structure Biological treatment of solid waste (composting and anaerobic digestion) Selected publications: Oliveira R, Azeredo, J, Teixeira, P. The importance of physicochemical properties in biofilm formation and activity. In Biofilms in Wastewater Treatment - An Interdisciplinary Approach, S Wuertz, PL Bishop, PA Wilderer (Eds), IWA Publishing, London, 211-231, 2003. • Azeredo J, Visser J, Oliveira R. Exopolymers in bacterial adhesion: interpretation in terms of DLVO and XDLVO theories. Colloids and Surfaces B: Biointerfaces 14, 141-148, 1999. • Azeredo J, Lazarova V, Oliveira R. Methods to extract the polymeric matrix of biofilms: a comparative study. Water Science and Technology 39, 243-250, 1999. • Teixeira P, Azeredo J, Oliveira R, Chibowski E. Interfacial interactions between nitrifying bacteria and mineral carriers in aqueous media determined by contact angle measurements and thin layer wicking. Colloids and Surfaces B: Biointerfaces 12, 69-75, 1998. • Oliveira, D R. Physico-chemical aspects of adhesion In Biofilms - Science and Technology, LF Melo, TR Bott, M Fletcher, B Capdeville (Eds), 45-58, Kluwer Academic Publishers, Dordrecht, 1992. • 8 DEPARTMENT OF BIOLOGICAL ENGINEERING FACULTY MEMBERS Nelson Manuel Viana da Silva Lima Full Professor Education: • • • Positions: • • • • • • • Ph.D. in Engineering Science (Biotechnology), UM, 1993 Master in Microbiology, UM, 1989 Graduation in Biology and Geology (Teacher Training), UM, 1983 Full Professor, (Integrated Science Department) UM, 2004Associate Professor, (Integrated Science Department) UM, 1997-2004 Assistant Professor, UM, 1993-1997 Teaching Assistant, UM, 1988-1993 Teaching Assistant, University of Aveiro, 1984-1988 Teaching Assistant, Polytechnic Institute of Viseu, 1983-1984 Teacher of Biology in Secondary School, 1982-1983 Main Teaching Areas: • • • • • Ecology (Childhood Study Institute) Natural Conservation (Childhood Study Institute) Environmental Microbiology/Micology Microbial Genetics Environmental Promotion and Education Research Activities: • • • Environmental Microbiology/Micology Environmental Promotion and Education Preservation of Micological Resources Selected publications: Paterson RRM, Venâncio A, Lima N. Solutions to Penicillium taxonomy crucial to mycotoxin research and health. Research in Microbiology 155, 507-513, 2004. • Nicolau A, Mota M, Lima N. Effect of different toxic compounds on ATP content and ACP activity in Tetrahymena pyriformis. Ecotoxicology and Environmental Safety 57, 129-135, 2004. • Dias N, Mortara RA, Lima N. Morphological and physiological changes in Tetrahymena pyriformis for the in vitro cytotoxicity assessment of Triton X- 100. Toxicology in Vitro 17, 357–366, 2003. • Martins MAM, Lima N, Silvestre AJD, Queiroz MJ. Comparative studies of fungal degradation of single or mixed bioaccessible reactive azo dyes. Chemosphere 52, 967-973, 2003. • Serra R, Abrunhosa L, Kozakiewicz Z, Venâncio A, Lima N. Use of ozone to reduce molds in a cheese ripening room. Journal of Food Protection 66, 2355-2358, 2003. • 9 DEPARTMENT OF BIOLOGICAL ENGINEERING FACULTY MEMBERS Maria Teresa Simões Campos Tavares Associate Professor Education: • • Positions: • • • Ph.D. in Engineering Science, UM, 1991 Graduation in Chemical Engineering, FEUP, 1982 Associate Professor, UM, 1999 Assistant Professor, UM, 1991-1999 Teaching Assistant, UM, 1983-1991 Main Teaching Areas: • • • • Chemical Reaction Engineering Separation Processes Wastewater Physico-Chemical Processing Applied Biocatalysis Research Activities: • • • • Biological Removal of Chromium from Industrial Effluents Methodologies for Remediation of Contaminated Soils Biossorbents for Industrial Applications Integrated Method for Heavy Metal Recovery and Reuse Selected publications: • Quintelas C, Tavares T. Lead (II) and iron (III) removal from aqueous solution: biosorption by a bacterial biofilm supported on granular activated carbon. Resource and Environmental Biotechnology 3, 193-202, 2002. • Quintelas C, Tavares T. Removal of chromium(VI) and cadmium(II) from aqueous solution by a bacterial biofilm supported on granular activated carbon. Biotechnology Letters 23, 1349-1353, 2001. • Tavares MT, Santos Silva I. Eliminacion de metales pesados de efluentes líquidos, Catalysadores y Adsorbentes para la Proteccíon Ambiental, CYTED 2000, 132-138, 2000. • Tavares MT, Alstrup I, Bernardo CAA. Coking and decoking during methanation and methane decomposition on Ni-Cu supported catalysts. Materials and Corrosion 50, 1, 1999. • Alstrup I, Tavares MT, Bernardo CA, Sorensen O, Rostrup-Nielsen JR. Carbon formation on nickel and nickel-copper catalysts. Materials and Corrosion 49, 367-372, 1998. 10 DEPARTMENT OF BIOLOGICAL ENGINEERING FACULTY MEMBERS Eugénio M. F. Campos Ferreira Associate Professor Education: • • • Positions: • • • Post-doctoral studies, Universitat Autónoma de Barcelona 1997-1998 Ph.D. in Chemical Engineering, FEUP Porto, 1995 Graduation in Chemical Engineering, FEUP Porto, 1986 Associate Professor, UM, 2001Assistant Professor, UM, 1995-2000 Teaching assistant, UM, 1991-95 Main Teaching Areas: • • • • • Control and Instrumentation Strategy of Process Engineering Water and Wastewater Treatment Air Pollution Biological Reactors Research Activities: (Bio)Process Systems Engineering in Biotechnological, Chemical, Biopharmaceutical and Environmental processes: • Image Analysis Applications in Wastewater Treatment and Biotechnology • Modelling, Monitoring, and Control of Bioprocesses • Knowledge-Based Expert Systems in Supervision and Control • Process Integration and Design for Pollution Prevention: Synthesis, Analysis and Optimization • Chemometrics in Environmental Processes – Environmetrics Selected publications: Rocha I, Ferreira EC. On-line simultaneous monitoring of glucose and acetate with FIA during high cell density fermentation of recombinant E. coli. Analytica Chimica Acta 462, 293-304, 2002. • Oliveira R, Ferreira EC, Azevedo SF. Stability, dynamics of convergence and tuning of observerbased kinetics estimators. Journal of Process Control 12, 311-323, 2002. • Perrier M, Feyo de Azevedo S, Ferreira EC, Dochain D. Tuning of observer-based estimators: theory and application to the on-line estimation of kinetic parameters. Control Engineering Practice 8, 377388, 2000. • Baeza J, Ferreira EC, Lafuente J. Knowledge-based supervision and control of WWTP: A real-time implementation. Water Science and Technology 41, 129-137, 2000. • Alves MM, Cavaleiro AJ, Ferreira EC, Amaral AL, Mota M, da Motta M, Vivier, H, Pons M-N. Characterization by image analysis of anaerobic sludge under shock conditions. Water Science and Technology 41, 207-214, 2000. • 11 DEPARTMENT OF BIOLOGICAL ENGINEERING FACULTY MEMBERS Maria João Lopes da Costa Vieira Associate Professor Education: • • Positions: • • • Ph.D. in Chemical and Biological Engineering, UM, 1995 Graduation in Chemical Engineering, FEUP, 1987 Associate Professor, UM, 2002Assistant Professor, UM, 1995- 2002 Teaching Assistant, UM, 1991-1995 Main Teaching Areas: • • • Transport Phenomena Transport Phenomena in Bioprocesses Elements on Biochemical Engineering Research Activities: Research area: Biofilms. On-going projects: • Control of biofilms using chemical methods • Survival of Helicobacter pylori in drinking water biofilms • Monitoring of biofilms in industrial equipment Selected publications: Teixeira P, Lopes Z, Azeredo J, Oliveira R, Vieira MJ. Prediction of bacterial adhesion to the surface of the milking machine. Food Microbiology 22, 247-251, 2005. • Azevedo N, Pacheco P, Keevil CA, Vieira MJ. Nutrient shock and incubation atmosphere influence the recovery of viable Helicobacter pylori from water. Applied and Environmental Microbiology 70, 490-498, 2004. • Simões M, Carvalho H, Pereira MO, Vieira MJ. Studies on the behaviour of Pseudomonas fluorescens biofilms after ortho-phthaldehyde treatment. Biofouling 19, 151-157, 2003. • Simões M, Pereira MO, Vieira MJ. Effect of different concentrations of ortho-phthalaldehyde on biofilms formed by Pseudomonas fluorescens under different flow conditions. Biofouling 19, 287-295, 2003. • Pereira MO, Vieira MJ, Melo LF. The role of kaolin particles in the performance of a carbamatebased biocide for water bacterial control. Water Environment Research 74, 235-241, 2002. • 12 DEPARTMENT OF BIOLOGICAL ENGINEERING FACULTY MEMBERS Francisco Miguel Portela da Gama Associate Professor Education: • • • Positions: • • • Ph.D. in Chemical and Biological Engineering, UM, 1996 Master in Biotechnology (Biochemical Engineering), IST, 1991 Graduation in Biochemistry, FCUP, 1986 Associate Professor, UM, 2003Assistant Professor, UM, 1996-2003 Teaching assistant, UM, 1992-1996 Main Teaching Areas: • • Physical Chemistry Enzymatic Engineering Research Activities: • • • • Carbohydrate binding modules Enzyme technology Polysaccharide/Fibres characterization and modification Protein delivery systems Selected publications: Pinto R, Moreira S, Mota M, Gama FM. Cellulose-binding domains adsorption to cellulose. Langmuir 20, 1409-1413, 2004. • Dourado F, Barros A, Mota M, Coimbra MA, Gama FM. Anatomy and cell wall polysaccharides of almond (Prunus dulcis D.A. Webb) seeds. Journal of Agricultural and Food Chemistry 52, 1364-1370, 2004. • Pala H, Mota M, Gama FM. Enzymatic versus chemical deinking of non-impact ink printed wastepaper. Journal of Biotechnology 108, 79-89, 2004. • Lemos MA, Teixeira JA, Domingues MRM, Mota M, Gama FM. The enhancement of the cellulolytic activity of Cellobiohydrolase I and Endoglucanase by the addition of cellulose binding domains derived from Trichoderma reesei. Enzyme and Microbial Technology 32, 35-40, 2003. • Lemos MA, Teixeira JA, Mota M, Gama FM. A simple method to separate cellulose binding domains of fungal cellulases after digestion by a protease. Biotechnology Letters 22, 703-707, 2000. • 13 DEPARTMENT OF BIOLOGICAL ENGINEERING FACULTY MEMBERS António Guerreiro de Brito Assistant Professor Education: • • Ph.D. in Chemical and Biological Engineering, UM, 1997 Graduation in Environmental Engineering, FCT-UNL, 1984 Positions: Head of Direcção Regional do Ordenamento do Território e dos Recursos Hídricos, Secretaria Regional do Ambiente, Governo Reg. Açores, 2000-2002 • Assistant Professor, UM, 1997- 2000 and (2002-) • Teaching Assistant, UM, 1987-1997 • Consulting Engineer, 1986-1988 • Engineer at Divisão de Hidráulica, Direcção Regional das Obras Públicas e Equipamento, Sec. Regional do Equipamento Social, Governo Reg. Açores, 1984-1987 • Main Teaching Areas: • • • • • Transport Phenomena Laboratory Polution Control Industrial Services Environmental Impact Assessment Laboratory of Water and Wastewater Technology Research Activities: Biofilms in wastewater systems (SBR, UASB/EGSB, design and modeling of wastewater treatment plants) • Biofilms in natural ecosystems (water quality modeling, eutrophication) and water resources management • Nitrogen removal, PAH degradation • Environmental engineering education • Selected publications: • Rodrigues AC, Brito AG, Melo LF. Post-treatment of a brewery wastewater using a sequencing batch reactor. Water Environment Research 73, 45-51, 2001. • Brito AG, Melo LF, Santana F. Environmental technology education in Portugal: analysis and perspectives. Water Science and Technology 41, 02, 23 –29, 2000. • Brito AG, Melo LF. Mass transfer coefficients within anaerobic biofilms: Effects of external liquid fluid velocity. Water Research 33, 3673-3678, 1999. • Brito AG, Melo LF. Operation of UASB and EGSB reactors with low strength acidified wastewaters: a simplified analysis of reaction and mass transfer effects. Environmental Technology 18, 35-44, 1997. • Brito AG, Rodrigues AC, Melo LF. Feasibility of a pulsed sequencing batch reactor with anaerobic aggregated biomass for the degradation of low strength wastewaters. Water Science and Technology 35, 193-198, 1997. 14 DEPARTMENT OF BIOLOGICAL ENGINEERING FACULTY MEMBERS Armando Albino Dias Venâncio Assistant Professor Education: • • Positions: • • Ph.D. in Chemical and Biological Engineering, UM, 1996 Graduation in Chemical Engineering, FEUP, 1991 Assistant Professor, UM, 1997Teaching assistant, UM, 1994-1997 Main Teaching Areas: • • Separation Processes Food Processes Research Activities: • • • Food Technology Food Safety: mycotoxins Aqueous Two-Phase separations Selected publications: Pereira M, Wu YT, Madeira P, Venâncio A, Macedo E, Teixeira JA. Liquid-Liquid Equilibrium Phase Diagrams of New Aqueous Two-Phase Systems: Ucon 50-HB5100 + Ammonium Sulfate + Water, Ucon 50HB5100 + Polyvinyl Alcohol + Water, Ucon 50-HB5100 + Hydroxypropyl Starch + Water and Polyethylene Glycol 8000 + Polyvinyl Alcohol + Water. Chemical Engineering Data 49, 43-47, 2004. • Serra R, Abrunhosa L, Kozakiewicz Z, Venâncio A. Black Aspergillus species as ochratoxin A producers in Portuguese wine grapes. International Journal of Food Microbiology 88, 63-68, 2003. • Abrunhosa L, Serra R, Venâncio A. Biodegradation of Ochratoxin A by Fungi Isolated from Grapes. Journal of Agriculture and Food Chemistry 50, 7493-7496, 2002. • Abrunhosa L, Paterson RRM, Kozakiewicz Z, Lima N, Venâncio A. Mycotoxin production from fungi isolated from grapes. Letters in Applied Microbiology 32, 240-242, 2001. • Wu YT, Pereira M, Venâncio A, Teixeira JA. Separation of endo-polygalacturonase using aqueous two-phase partitioning. Journal of Chromatography, 928, 23-29, 2001. • 15 DEPARTMENT OF BIOLOGICAL ENGINEERING FACULTY MEMBERS Maria Madalena dos Santos Alves Assistant Professor Education: • • • Positions: • • Ph.D. in Chemical and Biological Engineering, UM, 1998 Master in Biotechnology (Biochemical Engineering), IST, 1992 Graduation in Chemical Engineering, FEUP, 1987 Assistant Professor, UM, 1998Teaching assistant, UM, 1988-1998 Main Teaching Areas: • • • • Chemical Reaction Engineering Laboratory of Chemical Technology Biological Wastewater Treatment Laboratory of Environmental Microbiology Research Activities: • • • Anaerobic digestion of liquid, slurries and solid wastes Biological Wastewater Treatment: anaerobic and combined anaerobic- aerobic processes Environmental microbiology focused on anaerobic consortia Selected publications: Araya-Kroff P, Amaral AL, Neves L, Ferreira EC, Pons M-N, Mota M, Alves MM. Development of image analysis techniques as a tool to detect and quantify morphological changes in anaerobic sludge: I application to a granulation process. Biotechnology and Bioengineering 87, 184-193, 2004. • Amaral AL, Pereira MA, da Motta M, Pons M-N, Mota M, Ferreira EC, Alves MM Development of image analysis techniques as a tool to detect and quantify morphological changes in anaerobic sludge: II – application to a granule deterioration process triggered by the contact with oleic acid. Biotechnology and Bioengineering 87, 194-199, 2004. • Pereira MA, Roest K, Stams AJM, Mota M, Alves MM, Akkermans A. Molecular monitoring of microbial diversity of two EGSB reactors treating oleic acid. FEMS Microbiology Ecology 41, 95-103, 2002. • Alves MM, Mota Vieira JA, Álvares Pereira RM, Pereira MA, Mota M. Effects of lipids and oleic acid on biomass development in anaerobic fixed bed reactors. Part I: biofilm growth and activity. Water Research 35, 255-263, 2001. • Alves MM, Mota Vieira JA, Álvares Pereira RM, Pereira MA, Mota M. Effects of lipids and oleic acid on biomass development in anaerobic fixed bed reactors. Part II: Oleic acid toxicity and biodegradability. Water Research 35, 264-270, 2001. • 16 DEPARTMENT OF BIOLOGICAL ENGINEERING FACULTY MEMBERS Isabel Maria Pires Belo Assistant Professor Education: • • Positions: • • Ph.D. in Chemical and Biological Engineering, UM, 2000 Graduation in Chemical Engineering, FEUP, 1988 Assistant Professor, UM, 2000Teaching assistant, UM, 1990-2000 Main Teaching Areas: • • • Graphic Representation of Equipment and Processes Introduction to Chemical Processes Introduction to Process Engineering Research Activities: • • • Pressurized bioreactors: application on fermentation tecnhology High-pressure technology on biotechnological processes Oxidative stress response of microbial cells Selected publications: Coelho MAZ, Belo I, Pinheiro R, Amaral AL, Mota M, Coutinho JAP, Ferreira EC. Effect of hyperbaric stress on yeast morphology: study by automated image analysis. Applied Microbiology and Biotechnology 66, 318-324, 2004. • Belo I, Pinheiro R, Mota M. Fed-batch cultivation of Saccharomyces cerevisiae in a hyperbaric bioreactor. Biotechnology Progress 19, 665-671, 2003. • Pinheiro R, Belo I, Mota M. Growth and β-galactosidase activity in cultures of Kluyveromyces marxianus under increased air pressure. Letters in Applyed Microbiology 37, 438-442, 2003. • Pinheiro R, Belo I, Mota M. Oxidative stress response of Kluyeromyces marxianus to hydrogen peroxide, paraquat and pressure. Applied Microbiology and Biotechnology 53, 842-847, 2002. • Belo I, Pinheiro R, Mota M. Response of the Thermophile Thermus sp. RQ-1 to hyperbaric air in batch and fed-batch cultivation. Applied Microbiology and Biotechnology 53, 517-524, 2000. • 17 DEPARTMENT OF BIOLOGICAL ENGINEERING FACULTY MEMBERS António A. M. Soares de Oliveira Vicente Assistant Professor Education: • • Positions: • • Ph.D. in Chemical and Biological Engineering, UM, 1998 Graduation in Food Engineering, ESB-UCP, 1994 Assistant Professor, UM, 2001Teaching Assistant, UM, 1998-2001 Main Teaching Areas: • • • • Thermodynamics Control and Instrumentation Laboratory of Microbial Technology Laboratory of Biotechnology Research Activities: Fermentation Engineering (high cell density systems, bioreactor design, pH control analysis) • Ohmic heating (thermal processing of foods) • Selected publications: • Brányik T, Vicente AA, Cruz JMM, Teixeira JA. Continuous Primary Fermentation of Beer with Yeast Immobilized on Spent Grains – The Effect of Operational Conditions. Journal American Brewing Chemists 62, 29-34, 2004. • Castro I, Teixeira JA, Salengke S, Sastry SK, Vicente AA. Ohmic heating of strawberry products: electrical conductivity measurements and degradation kinetics. Innovative Food Science and Emerging Technologies 5, 27-36, 2004. • Klein J, Vicente AA, Teixeira JA. Hydrodynamics of a three-phase airlift reactor with an enlarged dual separator - application to high cell density systems. Canadian Journal Chemical Engineering 81, 433443, 2003. • Vicente AA, Mota M, Teixeira JA. Flocculation Bioreactors. In Multiphase Bioreactor Design, JMS Cabral, M Mota, Tramper J. (Eds), Taylor and Francis, 528, London 2001. • Brányik T, Vicente AA, Cruz JMM, Teixeira JA. Spent grains - a new support for brewing yeast immobilization. Biotechnology Letters 23, 1073-1078, 2001. • Castrillo JI, Vicente A, Teixeira JA, Ugalde U. On-line Estimation of Biomass and Growth-linked Product Synthesis through pH Control Analysis. In Research Advances in Biotechnology and Bioengineering Vol 1, 17, 2000 (ISBN:8187736003). 18 DEPARTMENT OF BIOLOGICAL ENGINEERING FACULTY MEMBERS Joana C. Valente R. Azeredo Assistant Professor Education: • • Positions: • • Ph.D. in Chemical and Biological Engineering, UM, 1998 Graduation in Biological Engineering, UM, 1994 Assistant Professor, UM, 2001Teaching Assistant, UM, 1998-2001 Main Teaching Areas: • • • Transport Phenomena Interfacial Phenomena Laboratory of Bioprocess Engineering Research Activities: • • Adhesion of microorganisms and surface phenomena Biological control of biofilms (using bacteriophages) Selected publications: Henriques M, Azeredo J, Oliveira R. Adhesion of Candida albicans and Candida dubliniensis to acrylic and hydroxyapatite. Colloids and Surfaces B: Interfaces 33,235-241, 2004. • Oliveira R, Azeredo J, Teixeira P. The importance of physicochemical properties in biofilm formation and activity. In Biofilms in Wastewater Treatment - An Interdisciplinary Approach. S Wuertz, PL Bishop, PA Wilderer (Eds) IWA Pubslishing, London, 211-231, 2003. • Azeredo J, Oliveira R. The role of hydrophobicity and exopolymers in initial adhesion and biofilm formation. In Biofilms in Medicine, Industry and Environmental Biotechnology - Characteristics, Analysis and Control, P Lens, AP Moran, T Mahony, P Stoodley, V O'Flaherty (Eds), IWA Publishing, London, 16-31, 2003. • Azeredo J, Pacheco AP, Lopes I, Oliveira R, Vieira MJ. Monitoring cell detachment by surfactants in a parallel plate flow chamber. Water Science and Technology 47, 77–82, 2003. • Nakari-Setala T, Azeredo J, Henriques M, Oliveira R, Teixeira J, Linder M, Penttila M. Expression of a fungal hyfrophobin in Saccharomyce cell wall: Effect on cell surface Properties and immobilisation. Applied and Environmental Microbiology 68:7, 3385-3391, 2002. • 19 DEPARTMENT OF BIOLOGICAL ENGINEERING FACULTY MEMBERS José Maria Marques Oliveira Assistant Professor Education: • • • Positions: • • Ph.D. in Chemical and Biological Engineering, UM, 2001 Pedagogic and Scientific Exam in Enology (Equivalent to Master’s Degree), UM, 1995 Graduation in Biological Engineering, UM, 1991 Assistant Professor, UM, 2001Teaching assistant, UM, 1991-2000 Main Teaching Areas: • • • Enology Laboratory of Instrumental Methods of Analysis Laboratory of Bioprocess Engineering Research Activities: • • Aromatic characterisation of “Vinhos Verdes” grape varieties Impact of winemaking technology on the aromatic profile of “Vinhos Verdes” Selected publications: Oliveira JM, Araújo IM, Pereira OM, Maia JS, Amaral AJ, Odete Maia M. Characterization and differentiation of five Vinhos Verdes grape varieties by monoterpenic compounds, Analytica Chimica Acta 513, 269-275, 2004. • Oliveira JM, Maia MO, Baumes RL, Bayonove CL. Free and bound aromatic components of Loureiro and Alvarinho grape varieties from the Vinhos Verdes region. Viticulture and Enology Science 55, 13-20, 2000. • Oliveira JM, Baumes RL, Bayonove CL, Maia MO. Différentiation des cépages portugais loureiro et alvarinho basée sur la composition monoterpénique libre. VIème Symposium International d’œnologie, Bordeaux, 10-12 Juin 1999. A. Lonvaud-Funel (Coord.), Editions Tec and Doc, pp. 152-156, 2000. • Oliveira JM, Maia MO, Raymond L, Baumes RL, Bayonove CL. Teneur en norisoprénooïdes en C13 des Cépages Portugais Loureiro et Alvarinho. XXIV Congrés Mondial de la Vigne et du Vin, 79ème Assemblé Générale de L’OIV, 5-9 Juillet, Mainz, Alemagne, 225-232, 1999. • Oliveira JM, Castro AR, Arantes J, Oliveira P, Maia MO. Production de vins mousseux a partir de Vinhos Verdes monovarietaux. XXIIIème Congrès Mondial de la Vigne et du Vin, Lisboa, Portugal, 22-27 Junho, 1998. • 20 DEPARTMENT OF BIOLOGICAL ENGINEERING FACULTY MEMBERS Regina Maria de Oliveira Barros Nogueira Assistant Professor Education: • • • Positions: • • Ph.D in Chemical and Biological Engineering, UM, 2002 Master in Environmental Technology, UM, 1996 Graduation in Biological Engineering, UM, 1992 Assistant professor, UM, 2002Teaching assistant, UM, 1993-2002 Main Teaching Areas: • • • • Laboratory of Fluids and Heat Transfer Transport Phenomena Laboratory Instrumental Methods of Analysis Laboratory of Chemical Technology Research Activities: • • • Kinetics and mass transfer processes in biofilms Environmental microbiology focused in nitrifying and heterotrophic bacteria Biofilms in freshwaters Selected publications: Nogueira R, Purkhold U, Melo L, Wagner M. Study of nitrifying bacteria diversity on biofilms using a molecular approach. In Biological Resource Centres and the Use of Microbes, N Lima, D Smith (Eds). Micoteca da Universidade do Minho, Braga,141-152, 2003. • Nogueira R, Melo LF, Purkhold U, Wuertz S, Wagner M. Microbial population dynamics versus nitrification performance in biofilm reactors: Effects of hydraulic retention time and the presence of organic carbon. Water Research 36:2, 469-481, 2002. • Garcia-López L, Nogueira R, Melo LF, Veiga M C. Studies of mass transfer coefficients in denitrifying biofilms. 1st World Water Congress of the International Water Association, IWA. Paris, 3-7 July, 2000. • Nogueira R, Lazarova V, Melo LF. Influence of dissolved oxygen on nitrification kinetics in a circulating bed biofilm reactor. Bioprocess Engineering 19, 441–449, 1998. • Lazarova V, Nogueira R, Manem J, Melo LF. Control of nitrification efficiency in a new biofilm reactor. Water Science and Technology 36, 31–41, 1997. • 21 DEPARTMENT OF BIOLOGICAL ENGINEERING FACULTY MEMBERS João Monteiro Peixoto Assistant Professor Education: • • • • Ph.D in Chemical and Biological Engineering, UM, 2003 Master in Environmental Technology, UM, 1996 Graduation in Biological Engineering, UM, 1993 Graduation in Nursing, Nursing School Calouste Gulbenkian, Braga, 1980 Positions: • • • Assistant Professor, UM, 2003Teaching Assistant, UM, 1994-2003 Monitor, UM, 1991-1994 Main Teaching Areas: • • • Environmental Engineering Thermodynamics Laboratory of Fluids and Heat Transfer Research Activities: • • Bioreactors for the treatment of VOCs Air pollution control Selected publications: Peixoto J, Mota M. Trickling filter versus biological plate tower in VOC removal. 10th European Congress on Biotechnology, Madrid, Spain, July 2001. • Peixoto J, Mota M. Biodegradation of toluene in a trickling filter. Bioprocess Engineering 19, 393397, 1998. • Peixoto J, Mota M. Volatile organic compounds evaporation chamber for the simulation of gas effluents in laboratory research. Biotechnology Techniques 11, 1-6, 1997. • 22 DEPARTMENT OF BIOLOGICAL ENGINEERING FACULTY MEMBERS Lucília Maria Alves Ribeiro Domingues Assistant Professor Education: • • • Positions: • • • PhD in Chemical and Biological Engineering, UM, 2001 Master in Biological Engineering, UM, 1997 Graduation in Chemical Engineering, FEUP, 1994 Assistant Professor, UM, 2003Teaching Assistant, UM, 2001-2003 Invited Assistant, UM, 2000-2001 Main Teaching Areas: • • • • Chemical Reaction Engineering Laboratory of Chemical Technology Microbial Technology Applied Environmental Microbiology Research Activities: • • Molecular Biotechnology - cloned gene expression - development of yeast strains for biotechnological processes (ethanol fermentation, heterologous protein production) High cell density fermentation systems Selected publications: Domingues L, Oliveira C, Castro I, Lima N, Teixeira JA. Production of β-galactosidase from recombinant Saccharomyces cerevisiae grown on lactose. Journal of Chemical Technology and Biotechnology 79, 809-815, 2004. • Domingues L, Lima N, Teixeira JA. An integrated approach for cheese whey lactose valorization. In Recent Research and Developments in Biotechnology and Bioengineering 5, 65-78, 2003. • Domingues L, Teixeira JA, Pentillä M, Lima N. Construction of flocculent Saccharomyces cerevisiae strain secreting high levels of Aspergillus niger β-galactosidase. Applied Microbiology and Biotechnology 58:5, 645-650, 2002. • Domingues L, Lima N, Teixeira JA. Alcohol production from cheese whey permeate using genetically modified flocculent yeast cells. Biotechnology and Bioengineering 72, 507-514, 2001. • Domingues L, Vicente AA, Lima N, Teixeira JA. Applications of yeast flocculation in biotechnological processes. Biotechnology and Bioprocess Engineering 5, 288-305, 2000. • 23 DEPARTMENT OF BIOLOGICAL ENGINEERING FACULTY MEMBERS Maria Olívia B. Pereira Assistant Professor Education: • • Positions: • • • Ph.D. in Chemical and Biological Engineering, UM, 2001 Graduation in Biological Engineering, UM, 1993 Assistant Professor, UM, 2003Teaching Assistant, UM, 2001- 2003 Invited Assistant, UM, 2000-2001 Main Teaching Areas: • • • • Transport Phenomena Laboratory of Fluids and Heat Transfer Biological Wastewater Treatment Laboratory of Environmental Technology Research Activities: • • • Biofilm prevention and control Disinfection and Sanitation Biofilm monitoring in industrial, food and medical equipment Selected publications: Simões M, Pereira MO, Vieira MJ. How the hydrodynamic conditions under which the biofilms are formed affect the effectiveness of aldehyde based biocides. In Biofilm Communities: Order from Chaos, McBain A, Allison D, Brading M, Richard A, Verran J, Walker J (Eds), Bioline, Cardiff, 191-203, 2003. • Simões M, Carvalho H, Pereira MO, Vieira MJ. Studies on the behaviour of Pseudomonas fluorescens biofilm after ortho-phthalaldehyde treatment. Biofouling 19, 151-157, 2003. • Pereira MO, Kuehn M, Wuertz S, Neu T, Melo L. Effect of flow regime on the architecture of a P. fluorescens biofilm, Biotechnology and Bioengineering 78, 164-171, 2002. • Pereira MO, Vieira MJ, Beleza VM, Melo LF. Reduction of biofouling in paper production processes by using a carbamate-based biocide as a retention agent. Pulp and Paper of Canada, 102, 4245, 2001. • Pereira MO, Vieira MJ. Effects of the interactions between glutaraldehyde and the polymeric matrix on the efficacy of the biocide against Pseudomonas fluorescens biofilms. Biofouling 17, 93-101, 2001. • 24 DEPARTMENT OF BIOLOGICAL ENGINEERING LABORATORIES 3. Laboratories Research Laboratories Lab. Instruments Lab. Chromatography Lab. Chemical Engineering Lab. Image Analysis Lab. Mycology & Molecular Biology Lab. Biofilms Lab. Applied Microbiology Lab. Enzyme Technol. & Bioseparation 25 Lab. Pilot Plants Lab. Fermentation Lab. Environmental Biotechnology Lab. Food Science & Technology DEPARTMENT OF BIOLOGICAL ENGINEERING LABORATORIES Research Laboratories Lab. Wastewater Treatment & Minimization Lab. Molecular Microbiology &Ecology Lab. Cell & Tissue Culture Pedagogical Laboratories Lab. Chemical Technology Lab. Food Technology Lab. Environmental Technology Lab. Bioengineering 26 Lab.Fluids & Heat Transfer Lab. Molecular Microbiology &Ecology DEPARTMENT OF BIOLOGICAL ENGINEERING EDUCATION 4. Education BIOLOGICAL ENGINEERING COURSE (GRADUATE DEGREE) The Biological Engineering course has a strong component of Chemical Engineering and aims at forming specialists -"Process Engineers"- for industries having predominantly biological, chemical or physicochemical processes. The acquired knowledge makes them capable of collaborating in industrial sectors of pollution control and quality control, or to get into a research career in those domains. The course is divided in two tracks. The division takes place at the end of the 3rd year, but in the 4th year there are still some common subjects, being the difference completely established in the 5th year, thus: • in the track of "Chemical and Food Technology", there is an emphasis in the food domain and in the 5th year there are some specific disciplines of this area - Food Processes, Enology, etc; • in the track of "Pollution Control", the 5th year is centred in treatment systems of wastewater, gaseous emissions and solid wastes, as well as in the project and modification of industrial processes in order to minimise pollution. The course is structured in order to set up an interface between Chemical Engineering and Biological Sciences, especially Microbiology. In the 1st year there is a predominance of basic sciences (Mathematics, Physics and Chemistry) in parallel with an Introduction to Informatics and Chemical Engineering. In the 2nd and 3rd years the stress is laid upon subjects of Chemical Engineering and Biological Sciences. The convergence of those two domains gives rise to the disciplines of specialisation in the 4th and 5th years. In the 4th year there is still a common core of disciplines, giving formation in fields like: Fermentation Technology, Recovery of Biological Products, Enzyme Technology and Process Control. The practical formation assumes a great importance, hence around 25% of the total number of classes are laboratory classes and the last semester of the course is a probation in industry. This period of training is supervised by a teacher from the University and a professional from the Industry. The disciplines of Project (in both tracks) of the 5th year have also the purpose of involving the students in industrial and/or research projects. This type of formation enables a wide range of professional careers in areas such as: • • • • • • • • food industry with fermentative processes (beer, wine, cheese, etc.); food industry having essentially physical or physico-chemical processes (biscuits, fruit juices, edible oils, etc.); pharmaceutical industry (antibiotics, steroids); industries of cellulose, tanning , cork, wood; chemical industries in general (organic and inorganic); water industries (mineral waters), water treatment plants and wastewater treatment plants; other companies concerned with industrial pollution control; Consulting and project enterprises. Director: Maria João Vieira 27 DEPARTMENT OF BIOLOGICAL ENGINEERING EDUCATION PROFESSIONAL SITUATION OF THE GRADUATES IN BIOLOGICAL ENGINEERING The total number of graduates in Biological Engineering since 1991 is 389. Only 4% of the graduates do not have a full time job and 15% are attending pos graduation courses like masters and Ph.D. 81% of Biological Engineers develop their professional activity mainly in companies (37%) and industries (18%). Research (17%) and public administration jobs (10%) also represent an important fraction of employment. employed 81% post graduation 15% enterprises 37% unemployed 4% university education 8% industry 16% local and regional technological administration laboratories centres 10% 5% 2% INTERNATIONAL STUDENTS EXCHANGE PROGRAMMES The international students exchange is done under the following programmes: • Erasmus/Socrates for academic training of students. • Leonardo Programme for industrial training of students. 28 research 17% high school education 5% DEPARTMENT OF BIOLOGICAL ENGINEERING EDUCATION SYLLABUS OF BIOLOGICAL ENGINEERING Hours / week Year Subjects T P SE L Total 1 S st 1º Physics Math Analysis I Laboratory of Chemistry Linear Algebra Organic Chemistry I Graphical Representations Math Analysis II Organic Chemistry II Analytical Chemistry Introduction to Computer Science Introduction to Chemical Processes Transport Phenomena Complementary Math Analysis Numerical Methods I Cellular Biology and Physiology General Biochemistry Thermodynamics Statistical Methods Numerical Methods II General Microbiology Laboratory of Fluids and Heat Physical Chemistry 3 0 0 5 5 3 3 0 0 6 0 0 0 0 3 3 0 2 3 0 0 5 0 2 0 0 3 5 0 1 0 0 2 3 0 3 3 0 0 0 6 3 0 0 3 0 6 2 0 0 3 0 5 1 1 0 2 0 4 2 2 0 0 0 4 27 30 2 3 0 0 5 5 2 3 0 0 5 0 2 2 0 0 4 0 2 0 0 2 4 0 1 0 0 2 3 0 3 2 0 0 5 0 2 2 0 0 0 4 2 2 0 0 0 4 2 0 0 3 0 5 0 0 0 3 0 3 3 2 0 0 0 5 26 26 2 3 0 0 5 5 2 1 0 2 5 0 1 0 0 3 4 0 2 2 0 0 4 0 2 0 0 3 5 0 3 2 0 0 5 0 2 0 0 3 0 5 2 0 0 3 0 5 3 2 0 0 0 5 0 0 0 6 0 6 28 26 TOTAL 3º Separation Processes Electricity and Magnetism Instrumental Methods of Analysis Analysis of Industrial Costs I Microbial Biochemistry Chemical Reaction Engineering I Electronics Applied Microbiology Chemical Reaction Engineering II Laboratory of Chemical Technology TOTAL 4º 5º Laboratory of Bioprocess Engineering Enzyme Engineering Transport Phenomena in Bioprocesses Economic Engineering I Operational Research I Fermentation Technology Separation Processes in Biotechnology Control and Instrumentation Strategy in Process Engineering Seminars Industrial Training 29 nd 2 TOTAL 2º 2 S 0 0 4 0 4 4 2 2 0 0 4 0 2 2 0 0 4 0 2 1 0 0 3 0 2 3 0 0 5 0 2 2 0 0 0 4 2 2 0 0 0 4 2 1 0 0 0 3 1 3 0 0 0 4 0 0 4 0 4 0 0 0 28 0 0 28 DEPARTMENT OF BIOLOGICAL ENGINEERING RESEARCH SYLLABUS OF BIOLOGICAL ENGINEERING - TRACK OF POLLUTION CONTROL Hours / week Subjects Year T P SE L Total 1 S 2 S 0 st 4º Introd. to Environmental Engineering Water and Wastewater Treatment I 3 0 0 3 6 3 0 0 3 0 6 26 25 TOTAL 5º Water and Wastewater Treatment II Air Pollution Solid Waste Treatment Project in Environmental Technology Environmental Impact Assessment nd 3 1 0 2 5 0 2 1 0 0 3 0 2 0 0 0 2 0 0 2 0 0 2 0 2 2 0 0 4 0 26 28 TOTAL SYLLABUS OF BIOLOGICAL ENGINEERING - TRACK OF FOOD AND CHEMICAL TECHNOLOGY Hours / week Subjects Year T P SE L Total 1 S st 4º Genetic Engineering Industrial Facilities Food Processes Pollution Enology Project Food Biotechnology TOTAL T: theoretical; P: practical; L: laboratorial SE: seminar 30 nd 2 0 0 3 5 0 2 3 0 0 0 5 25 24 2 4 0 4 6 0 2 2 0 0 4 0 2 0 0 2 4 0 0 2 0 0 2 0 2 2 0 0 4 0 28 28 TOTAL 5º 2 S DEPARTMENT OF BIOLOGICAL ENGINEERING EDUCATION BIOMEDICAL ENGINEERING COURSE (GRADUATE DEGREE) The curriculum of this course is composed of disciplines belonging to different areas of knowledge: Basic Sciences, Health and Life Sciences, Engineering Sciences (such as Technical Drawing, Transport Phenomena, Physical-Chemistry of Materials, etc.), Management (Cost Analysis, Operations Research, Hospital Management, etc.), Humanities (involving subjects such as Interpersonal relationship, Human Resources, Ethics, etc.) and Specific Technologies related with the different tracks offered as professional careers. The curriculum is distributed according to the following fields of knowledge: - Basic Sciences (Physics, Chemistry, Mathematics) – 12% - Health and Life Sciences (e.g., Anatomy and Physiology, Cellular and Molecular Biology) – 10% - Engineering Sciences (e.g., Technical Drawing, Transport Phenomena, Interfacial Phenomena, etc.) – 22% - Management – 8% - Humanities – 4.5% - Specific Technologies – 43.5% There will be a common core and, in the end of the 3rd year, students will have to choose among the 4 different tracks offered. For example, those students who will be interested in a career on Rehabilitation Engineering, will have to follow courses on, e.g., Biomechanics, where they will learn, besides the body mechanisms (muscle and bone), concepts of ergonomics. Clinical Engineering is the track of the responsibility of the Department of Biological Engineering The students will have a training period where they will be placed in medical device companies during the final semester of the course. Alternatively, they may develop a research project either inside the University of Minho or in other research Centres. The students will have to present a written report about the training period and to make an oral presentation before a jury. Director: Manuel Mota 31 DEPARTMENT OF BIOLOGICAL ENGINEERING EDUCATION SYLLABUS OF BIOMEDICAL ENGINEERING Year Hours/week Subjects T P Total L 1 S 2 S st 1º Physics I Mathematical Analysis I Physical-Chemistry Linear Algebra Introduction to Informatics Introduction to Biomedical Engineering Eng. Drawing and Graphs Mathematical Analysis II Organic Chemistry Physics II English Structure and Properties of Materials 2 2 0 4 0 2 2 0 4 0 2 2 0 4 0 2 2 0 4 0 2 2 0 4 0 2 0 0 2 0 2 2 0 0 4 2 2 0 0 4 2 2 0 0 4 2 2 0 0 4 2 0 0 0 2 2 2 0 0 4 22 22 2 2 0 4 0 2 2 0 4 0 2 2 0 4 0 2 2 0 4 0 2 2 0 4 0 2 2 0 4 0 2 2 0 0 4 2 2 0 0 4 2 2 0 0 4 2 2 0 0 4 2 2 0 0 4 2 2 0 0 4 0 0 4 0 4 24 28 TOTAL 2º Cellular and Molecular Biology Biochemistry Biomedical Statistics Fluid Mechanics Hospital Safety Electronics I Numerical Methods Physical Chemistry of Materials Electronics II Heat and Mass Transfer Interfacial Phenomena Anatomy and Physiology I Electronics Lab TOTAL 3º Cost Analysis Signal Processing Operational Research Bio-informatics Biomaterials Transport Phenomena Lab Humanities Elective I Clinical Facilities Reliability and Maintenance Medical Instrumentation Data Banks and Networks Materials Processing Anatomy andPhysiology II Materials Lab 2 2 0 4 0 2 2 0 4 0 2 2 0 4 0 2 2 0 4 0 2 0 0 2 0 0 0 4 4 0 2 0 0 2 0 2 2 0 0 4 2 2 0 0 4 2 0 0 0 2 2 2 0 0 4 2 0 0 0 2 2 2 0 0 4 24 24 4 TOTAL 4º 5º Topics in Hospital Management Project Humanities Elective II Humanities Elective III Biomedical Ethics Seminars Practical Training T: theoretic; P: practical; L: laboratorial 32 nd 4 3 0 0 0 3 0 6 0 0 6 2 0 0 2 0 2 0 0 0 2 2 0 0 2 0 0 0 2 2 0 0 0 14 0 14 DEPARTMENT OF BIOLOGICAL ENGINEERING EDUCATION SYLLABUS OF BIOMEDICAL ENGINEERING - TRACK OF CLINICAL ENGINEERING Year Hours/week Subjects T P Total L 1 S 2 S st 4º Biosensors I Cells and Tissues Culture I Bio-separations Instrumental Methods of Analysis Integrated Labs I Controlled Drug Release Cells and Tissues Culture II Liquid Water Treatment Solid Waste Treatment Integrated Labs II 3 0 0 3 0 3 0 0 3 0 3 0 0 3 0 0 0 4 4 0 0 0 4 4 0 3 0 0 3 0 3 0 0 0 3 3 3 0 0 0 0 4 TOTAL 5º Tissue Engineering Medical Imaging Systems Biosensors II Bio-compatibility Artificial Organs I Artificial Organs II TOTAL 33 nd 0 3 0 3 0 4 22 24 3 0 0 3 0 3 0 0 3 0 3 0 0 3 0 3 0 0 3 0 3 0 0 3 0 0 3 0 0 3 19 17 DEPARTMENT OF BIOLOGICAL ENGINEERING EDUCATION M.SC. IN ENVIRONMENTAL TECHNOLOGY This MSc course is designed to give a solid background in process design and retrofit in order to minimize on site pollution. It also gives formation on design and operation of water and wastewater treatment plants, as well as on solid waste treatment and disposal, air pollution control and environmental impact assessment. Qualifications for Admission The candidates should hold a degree in Biological, Civil, Mechanical, Metallurgical, Chemical, Production, Polymer or Textile Engineering. Other degrees that include subjects belonging to the field of the MSc course may also be accepted. The candidates must have 14 (in a scale 0/20) as minimal classification on their courses. Available Teaching Staff and Hardware Nine Lecturers from the University of Minho (coming out from 6 Departments). Other invited Lecturers from other Portuguese and foreign universities. The available facilities are the Laboratory of Molecular Microbiology & Ecology, Laboratory of Environmental Technology, Laboratory of Geographical Information Systems. The students may also have acess to the Department Library. Syllabus • Module A (10 weeks + exams) Quality of the Environment and Natural Resources Hydraulic Resources Planning Applied Environmental Microbiology Laboratories of Environmental Technology I • Module B (10 weeks + exams) Physico-chemical Processes in Liquid Medium Biological Processes in Liquid Medium Air Pollution Control Treatment of Solid Wastes • Module C (6 weeks + exams) Environmental Impact Analysis Strategies of Process Design Options (subject to be selected): Water Recovery in Textile Industries Environment and Energy Other Themes Laboratories of Environmental Technology II After having completed successfully the curricular modules, the students will have to do original research during one year. This research is presented under the form of an MSc thesis, which is examined by a qualified jury. Director: Rosário Oliveira 34 DEPARTMENT OF BIOLOGICAL ENGINEERING EDUCATION M.SC. IN ENVIRONMENTAL MANAGEMENT This MSc course is designed to give training in environmental management and pollution prevention in an industrial context. It is guided to the solution of problems in process industries as well as the acquisition of softskills in clean technologies and source pollution reduction contributing for a sustainable development. It covers the environmental issues surrounding industrial processes, including the legislative framework and explains how to minimise pollution and resource usage (green engineering) towards a cleaner production. Qualifications for Admission The candidates should hold a degree in Biological, Chemical, Environmental, Polymer, Materials, Production, Textile, Mechanical or Civil Engineering. Other degrees that include subjects belonging to the field of the MSc course may also be accepted. The candidates must have 14 marks (out of 20) as minimal classification on their courses. Available Teaching Staff and Hardware Lecturers from the University of Minho (coming from 9 Departments) and other invited lecturers from other Portuguese and foreign Universities. Additionally, experts from the industry and consulting firms will contribute. Library, computer facilities and laboratories are provided at the Department of Biological Engineering. Syllabus (40ETCS) • 1 Semester (13 weeks + exams) st Environmental Management Systems Products and Clean Processes Environmental Law and Policy Pollution Control Technologies I Energy and Environment Strategies of Process Integration • 2 Semester (13 weeks + exams) nd Quality, Safety, and Environment Pollution Control Technologies I Impact and Risk Analysis Recycling and Materials Environmental Auditing Economy and Environment After having completed successfully the curricular modules, the students will have to do original research during one year. This research is presented under the form of an MSc thesis, which is examined by a qualified jury. Director: Eugénio Ferreira 35 DEPARTMENT OF BIOLOGICAL ENGINEERING EDUCATION M.SC. IN BIOTECHNOLOGY - BIOPROCESS ENGINEERING Biotechnology is often considered as an area involving the use of biological organisms, of their subcellular components and of the products they excrete to human profit. Therefore, biotechnological processes appear either in biomass production and products or in waste treatment. In all cases, bioreactors are the central units where the principal transformations take place. Furthermore, bioseparations occur in downstream processing and constitute a fundamental step to obtain useful products. The MSc in Biotechnology - Bioprocess Engineering is thus formed by themes centred in bioreactors and bioseparations. Qualifications for Admission The candidates should hold a degree in Biological, Biochemical, Chemical, Food or Environmental Engineering, or Biochemistry, Biology, Chemistry and Pharmacy. Other degrees that include subjects belonging to the field of the MSc course may also be. The candidates must have 14 (in a scale 0/20) as minimal classification on their courses. Maximum allowable number of students: 20. Available Teaching Staff and Hardware Eleven Lecturers from the University of Minho and other invited lecturers from other Portuguese and foreign universities. All the laboratory and computer facilities are provided by the Department of Biological Engineering. Syllabus (23.5 credits) • 1 Semester (13 weeks + exams) st Introduction to Biotechnology Introduction to Bioprocess Engineering Biochemical Engineering Fundamentals Microbial Technology Transport Phenomena in Bioengineering Applied Biocatalysis • 2 Semester (13 weeks + exams) nd Biological Reactors Separation Processes in Bioengineering Laboratories of Bioengineering Option I - Environmental Biotechnology Option II - Food Biotechnology After having completed successfully the curricular modules, the students will have to do supervised original research during one year. This research is presented under the form of an MSc thesis, which is examined by a qualified jury. Director: José Teixeira 36 DEPARTMENT OF BIOLOGICAL ENGINEERING RESEARCH 5. Research The research staff besides the Faculty members listed in page 3 includes 13 Ph.D. members and 27 Ph.D. students. NON- PERMANENT STAFF Ph.D. Members Researcher Project Supervisor Alexander Yelshin Ana Cristina Rodrigues Filtration in Porous Media Polyciclic Aromatic Hydrocarbons Removal Using Sequencing Batch Biofilms Reactors Application of New Biological Models And Methods in the Determination of Quantitative Structure-Activity Relationships (Qsars) in Ecotoxicology Image Analysis in Biotechnological Processes Polysaccharides Bioactivity and Modification Metabolic Engineering Three Phase Air-Lift Reactors for Fermentation Anaerobic Biodegradation of Long Chain Fatty Acids Adhesion of Clinical Relevant Strains to Biomedical Materials Production of Dextran in Immobilised Biomass Reactors In Vitro Cytotoxicity Assessement of Toxic Compounds using N/a A.Brito E.Ferreira M.Gama E.Ferreira J.Teixeira M.Alves R.Oliveira J.Teixeira N.Lima A Simplified and Integrated Chemotaxonomy of Subgenus N.Lima Mycotoxins/Antibiotics Yeast Immobilization Systems in Brewing J.Teixeira Ana Paula Nicolau António Luís Amaral Fernando Dourado Isabel Cristina Rocha Jaroslav Klein Maria Alcina Pereira Maria do Pilar Teixeira Mariana Coelho Nicolina Marques Dias R. Russell M. Paterson Tomás Brányik Tetrahymena Pyriformis Penicillium Based on the Functional Characters of N.Lima Ph.D. Students Ana Cristina Veloso Ana Isabel Ferraz Cláudia Teresa Alves Cristina Maria Quintelas Cristina Vicente Diana Zita Sousa Fernanda Isabel Santos Inês Alexandra de Castro João Ricardo Pinto José Carlos G. Costa Lígia Raquel Rodrigues Luis Abrunhosa Pereira Manuel Vieira Simões Maria Adosinda Martins Maria Alberta Araújo Monitoring and Control of E. coli Fermentations Biological Removal of Heavy Metals Modeling of Biological Kinetics of Nitrification-Denitrification Modelling and Implementation of Biosorption Systems for Heavy Metals Valorisation of Proteins from Cheese Whey Construction and Molecular Characterization of Anaerobic Consortia Specialized for Lipids/LCFA Degradation Contribution for the Establishment of Filamentous Fungi Culture Collection Study of Ohmic Heating as a Processing Alternative to Conventional Thermal Treatments Surface Modification of Insoluble Polysaccharides The Microfaune of an Integrated Biological Wastewater Treatment Process: Application of Image Analysis and Molecular Biology Techniques in Process Control Production of Biological Anti-fouling Agents Detoxification of Foods containing mycotoxins Chemical Biofilm Control Biodegradability of Textile Dyes by Filamentous Fungi Biodegradability of Starch-Based Prosthetic Implants 37 E.Ferreira J.Teixeira /T.Tavares A.Brito T.Tavares J.Teixeira M.Alves N.Lima A.Vicente M.Gama/M.Mota E. Ferreira/M.Alves J.Teixeira/R.Oliveira A.Venâncio M.J.Vieira N.Lima M.Mota DEPARTMENT OF BIOLOGICAL ENGINEERING Maria Catarina Almeida RESEARCH Heterologous Proteins Production by Non-conventional Yeasts J.Teixeira Ph.D. Students Mariana Henriques Martinha Pereira Nuno Flipe Azevedo Nuno Miguel Cerca Nuno Miguel Reis Olga Pereira Pires Pablo Darío Araya Kroff Pedro Miguel Guimarães Ricardo Pereira Dias Rita Isabel Pinheiro Rita Maria Serra Adhesion of Candida sp. to Protheses and Epithelial Tissue Pectinase Production Behavior of Helicobacter pylori in Biofilms Physiology of Adhered Staphylococci to Indwelling Devices Novel Oscillatory Multiphase Bioreactors for Biotechnology Monitoring and Control of Wastewater Treatment Processes Aggregation of Anaerobic Microbial Consortia Physiology of Immobilised Yeast Cells Design of Chromatography Beds for the Separation fo Macromolecules with Different Molecular Conformations High Pressure Physiology of Yeasts Filamentous Fungi in Grapes and Wine Quality 38 R.Oliveira/J.Azeredo J.Teixeira M.J.Vieira J.Azeredo A.Vicente /J.Teixeira E.Ferreira/M.Alves M.Alves/M.Mota J.Teixeira/L.Domingues M.Mota M.Mota/I.Belo A.Venâncio DEPARTMENT OF BIOLOGICAL ENGINEERING RESEARCH RESEARCH ON GOING PROJECTS National Projects Person in charge Name of the Project Programme Starting date Manuel Mota Porous Media Properties and Separation of Macromolecules FCT POCTI January 2002 José Teixeira Gas-Liquid Mass Transfer in Multiphase Reactors FCT POCTI April 2003 José Teixeira Biological silage for milking cows ADI POCTI January 2003 José Teixeira Production of Enzymes for the Food Industry using Cheese Whey Lactose as Substrate for Fermentation ADI POCTI September 2002 José Teixeira Protein Partition in Aqueous Two-Phase Systems: Thermodynamic Characterization FCT POCTI September 2000 José Teixeira Development and Chemical, Structural and Rheological Characterization of Protein-Polysaccharide Mixed Aqueous Systems FCT POCTI February 2001 José Teixeira Goat and Sheep Milk Production in the Minho RegionCharacterization of the Industry and Demonstration of New Technologies AGRO INIA November 2001 Rosário Oliveira Adhesion of Candida albicans and Candida dubliniensis to Prosthetic Devices and Buccal Epithelial Cells FCT POCTI April 2003 Rosário Oliveira Anaerobic Digestion of Solid Wastes FCT POCTI December 2001 Rosário Oliveira Physiology of Staphylococci Attached to Indwelling Devices FCT POCTI March 2002 Teresa Tavares Integrated Process for Recovery and Reutilization of Heavy Metals FCT POCTI January 2001 Teresa Tavares Catalytic Reutilization of Zeolites Supported Biosorbents FCT POCTI Setembro 2003 Maria João Vieira On-line Monitoring of Industrial Biofilms FCT POCTI January 2001 Maria João Vieira Survival of Helicobacter pylori in Biofilms Formed in Drinking Water Distribution Systems. FCT POCTI January 2001 Maria João Vieira Chemical and Biological Control of Industrial Bofilms FCT POCTI March 2001 Maria João Vieira Cleaning and Disinfection in Dairy Industry Processes AGRO INIA December 2001 Miguel Gama FUNCARB - FUNctionalization of biomedical materials using a CARBhydrate binding domain FCT POCTI January 2004 Miguel Gama Immunobiological Studies of Microbial Virulence Immunomodulatory Proteins that Allow the Survival of the Secreting Microorganism in the Host FCT POCTI April 2001 Miguel Gama Upgrading Secondary Fibres with Cellulose Binding Domains FCT POCTI January 2002 Miguel Gama Cellulose Surface Modification using Enzymes Linked to CBDs FCT POCTI May 2003 Miguel Gama CBD-Text: Utilization of CBD’s Domains for the Improvement of Textile Fabrics ADI POCTI March 2002 INTERREG IIIB January 2004 António Brito Sustainable Systems for Wastewater Treatment in Rural Areas António Brito National Assessment on Water and Wastewaters systems Infraestructures and Economics INAG - MCOTA October 2003 Armando Venâncio Prevention of Contamination of Porto Wine and Vinho Verde by Fungi AGRO INIA February 2002 39 DEPARTMENT OF BIOLOGICAL ENGINEERING RESEARCH Research On Going Projects National Projects Person in charge Name of the Project Programme Starting date Madalena Alves Integration of Image Analysis, FISH Techniques and Physiological Activity to Monitor Anaerobic Digestion Processes FCT POCTI April 2003 Madalena Alves Photoassisted Electrochemical Oxidation of Lipidic Compounds as a Percursor to Anaerobic Treatment FCT POCTI March 2002 Madalena Alves Integrated Treatment of Textil Wastewater by Anaerobic/Aerobic/Advanced Oxidation Processes ADI POCTI November 2003 Madalena Alves Conception, Development and Construction of a Process/Reactor for Treatment and Energetic Valorization of Cow Manure ADI POCTI November 2003 António Vicente Improvement of a Fruit Pulp Production Line – Introduction of Ohmic Heating Technology as a Processing Alternative ADI POCTI December 2003 António Vicente Improvement of Enzyme-Modified Cheeses Towards Functionality FCT POCTI December 2000 Joana Azeredo Biofilms in Contact Lenses: Formation and Control FCT POCTI May 2003 José Maria Oliveira Determination of Complex Rootstock/Scion Most Important Effects on Yield And Quality of Vinhos Verdes AGRO INIA December 2001 António Brito Lake Restoration and Requalification Measures - Furnas DROTRH/SRA October 2003 António Brito and Regina Nogueira Regional Plan for Sustainable Development of Azores DRA/SRA October 2003 Name of the Project Programme Starting date BI-EURAM II: “Industrial Biotechnology” ALFA 2003 José Teixeira TACTS-META: TransAtlantic Student Mobility ALFA December 2002 José Teixeira ISEKI-Food: Integrating Safety and Environmental Knowledge Into Food Studies towards European Sustainable Development ERASMUS October 2002 Nelson Lima COMBOW: Control Of Mycological Contaminations in BOttled Water QLK1-CT October 2002 Maria João Vieira SAFER - Surveillance and Control of Microbiological Stability in Drinking Water Distribution Networks EU EESD-EN November 2002 Miguel Gama CBD-Hybrids: Engineering of Hybrid Ptoteins Containing Cellulose Binding Domains-Novel Biotechnological Tools for Pulp, Paper and Textile Industry EU QL-KA3 July 2001 Armando Venâncio Wine-Ochra Risk - Assessment and Integrated Ochratoxin A (OTA) Management in Grape And Wine EU QL-KA1 May 2001 António Vicente FOODPRO: Ohmic Heating for FOOD PROcessing EU Craft March 2004 International Projects Person in charge Manuel Mota Eugénio Ferreira Armando Venâncio 40 DEPARTMENT OF BIOLOGICAL ENGINEERING RESEARCH PH.D. AND M.SC. THESES Ph.D. Theses Concluded (Since 2001) Rita Isabel Couto Pinheiro: Pressure effect on yeast physiology. University of Minho. June 2004 Supervisors: Manuel Mota and Isabel Belo. Luís San Miguel Bento: Study of the Influence of Colourants on the Sugar Refinery Processing, University of Minho, December 2003 Supervisor: Manuel Mota. Nicolina M. Dias: Cytological and Physiological Studies on Protozoa in Contact with Toxic Compounds, University of Minho, November 2003 Supervisor: Nelson Lima. Isabel Cristina de Almeida Pereira da Rocha: Model-based Strategies for the Computer-Aided Production of Recombinant Human Proteins, University of Minho, November 2003 Supervisor: Eugénio Ferreira. Carla M. B. Guimarães: Biological Treatment of Effluents from the Regeneration of Ion Exchange Systems, University of Minho, November 2003 Supervisors: Manuel Mota and Rosario Oliveira. Maria Alcina Alpoim Pereira: Anaerobic biodegradation of long chain fatty acids, University of Minho, October 2003 Supervisors: Madalena Alves and Manuel Mota. Fernando O. Querióz Dourado: Enzymatic Extraction of Almond Oil, University of Minho, October 2003 Supervisors: Miguel Gama and Manuel Mota. Filipa Lopes: Interaction between Corrosion and Biofouling, University of Minho, October 2003 Supervisors: Luis Melo and Rosario Oliveira. António Luís P. Amaral: Image Analysis in Biotechnological Processes, University of Minho, September 2003 Supervisors: Eugénio Ferreira and Manuel Mota. João Peixoto: Biological Removal of VOC’s, University of Minho, February 2003 Supervisor: Manuel Mota. Ana Cristina Rodrigues: PAH degradation using a sequencing batch biofilm reactor, University of Minho, February 2003 Supervisor: António Brito. Regina Nogueira: Nitrification and Denitrification Bioreactors, University of Minho, December 2002 Supervisors: Luis Melo and António Brito. Carla Maria Duarte Freitas: Hydrodynamic Studies of Airlift Bioreactors, University of Minho, October 2002 Supervisor: José A. Teixeira Helena Pala Dias de Sousa: Utilization of Xylanases and Celluloses in Paper Fibre Recycling, University of Minho, September 2002 Supervisors: Miguel Gama and Manuel Mota. Maria do Pilar Araújo Teixeira: Aerobic Denitrification in Fixed Bed and Fluidised Reactors, University of Minho, September 2002 Supervisor: Rosario Oliveira Ana Nicolau: Pollution Biotechnological Monitoring: Protozoa Physiological Response to the Presence of Toxic Substances, University of Minho, April 2002 Supervisor: Nelson Lima José Maria Oliveira: Varietal and Fermentation Flavours that Typify the “Vinhos Verdes”, University of Minho, September 2001. Supervisor: M.Odete Maia. Maria Olívia B. Pereira: Comparison of the Efficacy of two Biocides, Carbamate and Glutaraldehyde in Biofilm Systems, University of Minho, September 2001. Supervisors: Luís de Melo and Maria João Vieira. José Manuel Gomes da Silva Neves: Large Scale Production of Entomoparasite Nematodes, University of Açores, March 2001. Supervisor: M. Mota. Lucília Maria Alves Ribeiro Domingues: Flocculent Saccharomyces cerevisiae Strains Genetically Modified for Lactose Utilization: Molecular Construction and Biotechnological Application, University of Minho, February 2001. Supervisors: José A.Teixeira and Nelson Lima. 41 DEPARTMENT OF BIOLOGICAL ENGINEERING RESEARCH MSc Theses Concluded (Since 2001) Eugénia Gonçalo: Anaerobic digestion of solid wastes (2004) Supervisors: Rosário Oliveira and Madalena Alves. Bárbara Cachada Cardoso: Antifungal susceptibility of biofilms vs planktonic cells of Candida albicans and Candida dubliniensis (2004) Supervisor: Joana Azeredo. Ângela Abreu: Monitoring microbial diversity of activated sludge by in-situ hibridization (2004) Supervisors: Madalena Alves and Ana Nicolau. Sanna Maria Sillankorva: Biofilm control by bacteriophages (2004) Supervisor: Joana Azeredo. Egídio Manuel Pires Torrado: Virulence Inducer Proteins that Allow the Survival of the Microorganisms in the Host (2003) Co-Supervisor: Miguel Gama. António Manuel do Vale Alves Velho: Integrative Transformation of Flocculent S.cerevisiae for β-galactosidase Production (2003) Supervisors: Nelson Lima and Lucília Domingues. João Pedro Carvalho de Oliveira de Magalhães Lançós: Production and Purification of Ochratoxin A (2003) Supervisor: Armando Venâncio. Helena Virgínia Sousa Ribeiro: Effect of Moderated Pressures on the Activity of Microbial Enzymes (2003) Supervisor: Isabel Belo. Helena Carvalho: Characterization of the Antimicrobial Efficiency of Ortho-phthalaldehyde Against Pseudomonas fluorescens Suspended Cultures (2003) Supervisors: Maria João Vieira and Maria Olivia Pereira. Ricardo Pereira Dias: Preparation and Characterisation of Controlled Particulate Mixtures with Glass Beads of Different Sizes (2003) Supervisor: Manuel Mota. Pedro António Palma Madeira: Thermodynamic Characterisation of Protein Partition in Aqueous Two Phase systems (2003) Supervisor: José A. Teixeira. Inês Alexandra Casaca Lage de Castro: Application of Ohmic Heating Technology to the Thermal Treatment of Strawberry Pulps (2002) Supervisor: António Vicente. Lúcia Neves: Characterization of Anaerobic Granular Sludge: Image Analysis, FISH and Histological Techniques (2002) Supervisors: Madalena Alves and Eugénio Ferreira. M.C.C. Rodrigues: Anaerobic Biodegradation of Rabbit-Breeding manure (2002) Supervisor: Madalena Alves. Ricardo Pinto: Use of Cellulose Binding Domains (CBDs) for the Interfacial Modification of Cellulose (2002) Supervisor: Miguel Gama. Teresa P. Costa: Start up and Operation of a Pilot Sequencing Batch Reactor for Municipal Wastewater Treatment (2002) Supervisor: António G. Brito Isabel A. Lopes: Surfactant Application to Control Biofilms Formed by P. fluorescens (2002) Supervisors: Maria João Vieira and Joana Azeredo. Sandra R. V. Lameiras: Development of Biossorbents on Monolitic Supports and on Natural Zeolites (2002) Supervisor: Teresa Tavares. Sandra C. T. Trindade: Development of Catalysts for Selective Reduction of Nox (2002) Supervisor: Teresa Tavares. Celso Santos: Enzimatic Synthesis os Mannose Oligosaccharides (2001) Supervisor: Miguel Gama. Ana Margarida Santos: Impact of the Ambiguity of the Genetic Code of S.cerevisiae in Adhesion and Surface Properties (2001) Supervisor: Joana Azeredo. Patrícia Taborda Gonçalves: Inertization of fly ashes (2001) Supervisor: Rosário Oliveira. Cristina M. C. Quintelas: Recover and Reutilization of Heavy Metals (2001) Supervisor: Teresa Tavares. Lígia R. M. Rodrigues: Valorisation of Cheese Whey Proteins (2001) Supervisor: José António Teixeira. Luís Abrunhosa Pereira: Isolation of Fungi and Production of Mycotoxins in Grapes (2001) Supervisor: Armando Venâncio. Isabel Maria Carneiro Ratão Production of Extracellular Proteins – Implementation of On-line Analysis Systems (2001) Supervisor: António Vicente. 42 DEPARTMENT OF BIOLOGICAL ENGINEERING RESEARCH 6. List of Publications (since 2002) This list integrates only books, peer reviewed papers and publications in book chapters. BOOKS 1. Baptista P, Venâncio A. Os Perigos para a Segurança Alimentar no Processamento de Alimentos, Forvisão Consultoria em Formação Integrada, Guimarães, 125 pp, 2003 (ISBN: 972-99099-3-8). 2. Cerdeira A, Venâncio A, Alves A, Barros P, Simões T. Impacto da Contaminação Fúngica sobre a Competitividade de Vinhos - Ocratoxina A, Micoteca da Universidade do Minho, Braga, 100 pp, 2003 (ISBN: 972-97916-2-7). 3. Gama FM, Aires-Barros MR, Cabral J. Engenharia Enzimática, Lidel Edições Técnicas, Lisboa, 272 pp, 2003 (ISBN: 972-757-272-3). 4. Lima N, Mota M. Biotecnologia: Fundamentos e Aplicações, Lidel Edições Técnicas, Lisboa, 505 pp, 2003 (ISBN: 972-757-797-2). 5. Lima N, Smith D. Biological Resource Centres and the Use of Microbes, Micoteca da Universidade do Minho, Braga, 418 pp, 2003 (ISBN 972-97916-3-5). 6. Ferreira EC, Rodrigues E. Fontes de Informação em Ambiente, Centro Atlântico, V.N. Famalicão, 200 pp, 2002 (ISBN: 972-8426-58-5). 7. Santos IM, Venâncio A, Lima N. Ecologia dos Fungos, Micoteca da Universidade do Minho, Braga, 110 pp, 2002 (ISBN: 972-97916-1-9). BOOK CHAPTERS 1. Aires-Barros MR, Gama FM, Videira A. Biocatálise em meios não-convencionais. In Engenharia Enzimática, FM Gama, MR Aires-Barros, J Cabral (Eds), Lidel Edições Técnicas, Lisboa, 141-162, 2003. 2. Araújo M, Oliveira JM, Maia, MO, Baumes, RL. Soil and climatic influence on the free and glycosidically bound volatiles of grapes from two white varieties from vinhos verdes region. In I International Symposium on Grapevine Growing, Commerce and Research, JC Sequeira, OA de Sequeira (Eds), International Society for Horticultural Science, Acta Horticulturae 652, 205-212, 2004. 3. Azeredo J, Oliveira R. The role of Hydrophobicity and Exopolymers in Initial Adhesion and Biofilm Formation. Ch. 2 In Biofilms in Medicine, Industry and Environmental Biotechnology - Characteristics, Analysis and Control, P Lens, AP Moran, T Mahony , P Stoodley, V O'Flaherty (Eds), IWA Publishing, London, 16-31, 2003. 4. Azevedo N, Vieira MJ, Keevil CW. Development of Peptide Nucleic Acid Probes to Detect Helicobacter pylori in Diverse Species Potable Water Biofilms. In Biofilm Communities: Order from Chaos, A McBain, D Allison, M Brading, A Rickard, J Verran, J Walker (Eds), Bioline, Cardiff, 105-111, 2003. 5. Cabral J, Gama FM, Aires-Barros MR. Introdução. In Engenharia Enzimática, FM Gama, MR Aires-Barros, J Cabral (Eds), Lidel Edições Técnicas, Lisboa, 1-12, 2003. 6. da Motta M, Amaral AL, Casellas M, Pons MN, Dagot C, Roche N, Ferreira EC, Vivier H. Characterisation of Activated Sludge By Automated Image Analysis: Validation on Full-Scale Plants, Computer Applications. In Biotechnology 2001, D Dochain, M Perrier (Eds), Pergamon Press, Oxford, 427-431, 2002. 43 DEPARTMENT OF BIOLOGICAL ENGINEERING RESEARCH 7. Dias N, Vandrovcova M, Kideryová L, Novotny C, Lima N. Use of protista and algae to study toxicity of dye compounds. In Biological Resource Centres and the Use of Microbes, N Lima, D Smith (Eds), Micoteca da Universidade do Minho, Braga, 71-85, 2003. 8. Domingues L, Gama FM, Videira A. Engenharia de Proteínas. In Engenharia Enzimática, FM Gama, MR Aires-Barros, J Cabral (Eds), Lidel Edições Técnicas, Lisboa, 97-120, 2003. 9. Domingues L, Lima N, Teixeira JA. An integrated approach for cheese whey lactose valorization. In Recent Research and Developments in Biotechnology and Bioengineering 5, 65-78, 2003. 10. Ferreira EC, Teixeira JA. Biorreactores. In Biotecnologia: Fundamentos e Aplicações, Lima N, Mota M (Eds), Lidel Edições Técnicas, Lisboa, 165-194, 2003. 11. Gama FM, Fonseca LP, Aires-Barros MR. Aplicações de enzimas. In Engenharia Enzimática, FM Gama, MR Aires-Barros, J Cabral (Eds), Lidel Edições Técnicas, Lisboa, 209-226, 2003. 12. Henriques M, Azeredo J, Oliveira R, Adhesion of Candida albicans and Candida dubliniensis to Oral Surfaces. In Biofilm Communities: Order from Chaos, A McBain, D Allison, M Brading, A Rickard, J Verran, J Walker (Eds), Bioline, Cardiff, 51-60, 2003. 13. Kelley J, Paterson RR. Comparison of ergosterol to other methods for determinations of Fusarium graminearum biomass in water as a model system. In Biological Resource Centres and the Use of Microbes, N Lima, D Smith (Eds), Micoteca da Universidade do Minho, Braga, 235-239, 2003. 14. Kozakiewicz Z, P Battilani, Cabanes J, Venâncio A, Logrieco A, Tjamos E, Lichter A, Magan N, Sanchis V, Lebrihi A, Zinzani G, Minguez S. Making Wine Safer: the case of Ochratoxin A. In Meeting the Mycotoxin, D Menace, H Barug, R van Egmond, T Lopez-Garcia, Van Osenbruggen, A Visconti (Eds), Wageningen Academic Publishers, Wageningen, 131-140, 2003. 15. Lima N, Correia A. Genética Clássica Aplicada. In Biotecnologia: Fundamentos e Aplicações, N Lima, M Mota (Eds), Lidel Edições Técnicas, Lisboa, 109-124, 2003. 16. Lima N, Mota M. Biotecnologia dos Ambientes Aéreos e Confinados. In Biotecnologia: Fundamentos e Aplicações, N Lima, M Mota (Eds), Lidel Edições Técnicas, Lisboa, 109-124, 2003. 17. Macedo ÂC, Venâncio A, Malcata FX. Biotecnologia dos Alimentos. In Biotecnologia: Fundamentos e Aplicações, N Lima, M Mota (Eds), Lidel Edições Técnicas, Lisboa, 431-472, 2003. 18. Martins MAM, Queiroz MJ, Lima N. Biodegradação de Corantes Azo Bioacessíveis por Fungos Lenhinolíticos, In Ecologia dos Fungos, IM Santos, A Venâncio, N Lima (Eds), Micoteca da Universidade do Minho, Braga, 87-98, 2002. 19. Melo LF, Vieira MJ. Effect of Clay Particles on Biofilm Composition and Reactor Efficiency. In Biofilms in Wastewater Treatment - An Interdisciplinary Approach, S Wuertz, PL Bishop, PA Wilderer (Eds) IWA Pubslishing, London, 325-342, 2003. 20. Mendonça C, Abrunhosa L, Serra R, Venâncio A. A contaminação fúngica e a ocratoxina A em vinhos. In: Impacto da Contaminação Fúngica sobre a Competitividade de Vinhos - Ocratoxina A, A Cerdeira, A Venâncio, A Alves, P Barros, T Simões (Eds), Micoteca da Universidade do Minho, Braga, 9-28, 2003. 21. Mota M, Novais JM, Controlo da Poluição Aquática. In Biotecnologia: Fundamentos e Aplicações, N Lima, M Mota (Eds), Lidel Edições Técnicas, Lisboa, 301-319, 2003. 22. Mota M, The importance of ex situ preservation for the utilization of microbiological resources, In Biological Resource Centres and the Use of Microbes, N Lima, D Smith (Eds), Micoteca da Universidade do Minho, Braga, 21-29, 2003. 44 DEPARTMENT OF BIOLOGICAL ENGINEERING RESEARCH 23. Nogueira R, Melo LF, Purkhold U, Wuertz S, Wagner M. Nitrifying and heterotrophic population dynamics in biofilm reactors: effects of hydraulic retention time and the presence of organic carbon, In Modern Scientific Tools in Bioprocessing, P Wilderer, S Wuertz (Eds), Series Developments in Water Science 49, Elsevier, 2002. 24. Nogueira R, Purkhold U, Melo L, Wagner M. Study of nitrifying bacteria diversity on biofilms using a molecular approach, In Biological Resource Centres and the Use of Microbes, N Lima, D Smith (Eds), Micoteca da Universidade do Minho, Braga, 141-152, 2003. 25. Oliveira R, Azeredo J, Teixeira P. The Importance of Physicochemical Properties in Biofilm Formation and Activity. In Biofilms in Wastewater Treatment - An Interdisciplinary Approach, S Wuertz, PL Bishop, PA Wilderer (Eds), IWA Pubslishing, London, 211-231, 2003. 26. Pala H, Pinto R, Duarte AP, Mota M, Gama FM. Cellulose-Binding domains as tools for paper recycling. In Applications of Enzymes Tolignocellulosics, ACS Symposium Series, SD Mansfield, JN Saddler (Eds), 105115, 2003. 27. Paterson RR, Venâncio A, Lima N. Mycotoxins - the experience and expertise of Micoteca da Universidade do Minho, In Biological Resource Centres and the Use of Microbes, N Lima, D Smith (Eds), Micoteca da Universidade do Minho, Braga, 217-233, 2003. 28. Paterson RR. The isoepoxydon dehydrogenase gene of the patulin biosynthetic pathway, patulin detection, and the utility of species names in patulin-producing fungi, In Biological Resource Centres and the Use of Microbes, N Lima, D Smith (Eds), Micoteca da Universidade do Minho, Braga, 259-266, 2003. 29. Peito, A, Venâncio, A. An Overview of Mycotoxins and Toxigenic Fungi in Portugal. In An Overview on Toxigenic Fungi and Mycotoxins in Europe, A Logrieco, A Visconti (Eds), Kluwer Academic Press, 173-184, 2004. 30. Rocha I, Ferreira EC. An Integrated System for Advanced Monitoring and Control of Fed-batch Fermentations of Recombinant E. coli, In Computer Applications in Biotechnology 2001, D Dochain, M Perrier (Eds), Pergamon Press, Oxford, 349-354, 2002. 31. Rocha M, Neves J, Rocha I, Ferreira EC. Evolutionary algorithms for optimal control in fed-batch fermentation processes. In Lecture Notes in Computer Science, vol. 3005: Applications of Evolutionary Computing, Springer Verlag, 84-93, 2004. 32. Santos IM, Lima N, Ryan MJ. Smith D. Metabolic Response of Filamentous Fungi to Preservative Stress, In Ecologia dos Fungos, IM Santos, A Venâncio, N Lima (Eds), Micoteca da Universidade do Minho, Braga, 55-66, 2002. 33. Santos IM, Lima N. Correlation between a deep clearing test and quantitative methods for lipolytic activity of filamentous fungi, In Biological Resource Centres and the Use of Microbes, N Lima, D Smith (Eds), Micoteca da Universidade do Minho, Braga, 99-104, 2003. 34. Serra R, Abrunhosa L, Venâncio A, Ecology of mycotoxin producing fungi present in wine grapes, In Biological Resource Centres and the Use of Microbes, N Lima, D Smith (Eds), Micoteca da Universidade do Minho, Braga, 251-258, 2003. 35. Serra R, Abrunhosa L, Venâncio A. Micoflora das Uvas e Micotoxinas, In Ecologia dos Fungos, IM Santos, A Venâncio, N Lima (Eds), Micoteca da Universidade do Minho, Braga, 47-54, 2002. 36. Simões M, Pereira MO, Vieira MJ. How the Hydrodynamic Conditions under which Biofilms are Formed Affect the Effectiviness of Aldehyde-based Biocides, In Biofilm Communities: Order from Chaos, A McBain, D Allison, M Brading, A Rickard, J Verran, J Walker (Eds), Bioline, Cardiff, 191-203, 2003. 45 DEPARTMENT OF BIOLOGICAL ENGINEERING RESEARCH 37. Taipa A, Gama FM. Estrutura e função dos enzimas. In Engenharia Enzimática, FM Gama, MR AiresBarros, J Cabral (Eds), Lidel Edições Técnicas, Lisboa, 13-66, 2003. 38. Valente L, Santos IM, Lima N. Implementação de Técnicas para Avaliação do Potencial Degradativo de Compostos Naturais por Fungos, In Ecologia dos Fungos, IM Santos, A Venâncio, N Lima (Eds), Micoteca da Universidade do Minho, Braga, 67-82, 2002. PEER-REVIEWED PAPERS 1. Abrunhosa L, Serra R, Venâncio A. Biodegradation of Ochratoxin A by Fungi Isolated from Grapes. Journal of Agriculture and Food Chemistry, 50:25, 7493-7496, 2002. 2. Almeida C, Brányik T, Moradas-Ferreira P, Teixeira JA. Continuous production of pectinase by immobilized yeast cells on spent grains. Journal of Bioscience and Bioengineering 96:6, 513-518, 2003. 3. Almeida C, Queirós O, Wheals A, Teixeira JA, Moradas-Ferreira P. Acquisition of flocculation phenotype by Kluyveromyces marxianus when overexpressing GAP1 gene encoding an isoform of glyceraldehyde-3phosphate dehydrogenase, Journal of Microbiological Methods 55, 433-440, 2003. 4. Alves C, Melo LF, Vieira MJ. Influence of medium composition on the characteristics of a denitrifying biofilm formed by Alcaligenes denitrificans in a fluidised bed reactor. Process Biochemistry 37:8, 837845, 2002. 5. Amaral AL, da Motta M, Pons MN, Vivier H, Mota M, Ferreira EC. Survey of Protozoa and Metazoa Populations in Wastewater Treatment Plants by Image Analysis and Discriminant Analysis. Environmetrics 15, 381-390, 2004. 6. Amaral AL, Pereira MA, da Motta M, Pons M-N, Mota M, Ferreira EC, Alves MM. Image Analysis as a Tool to Detect and Quantify Morphological Changes in Anaerobic Sludge: II. Application to a Granule Deterioration Process Triggered by the Contact with Oleic Acid. Biotechnology and Bioengineering 87, 194-199, 2004. 7. Araújo MA, Cunha AM, Mota M. Changes in morphology of starch-based prothestic thermoplastic material during enzymatic degradation. Journal of Biomaterials Science Polymer Edition 15, 1263-1280, 2004. 8. Araújo MA, Cunha AM, Mota M. Enzymatic Degradation of Starch based Thermoplastic Compounds used in Protheses: Identification of the Degradation Products in Solution, Biomaterials 25, 2687-2693, 2004. 9. Araya-Kroff P, Amaral AL, Neves L, Ferreira EC, Pons M-N, Mota M, Alves MM. Image Analysis as a Tool to Detect and Quantify Morphological Changes in Anaerobic Sludge: I. Application to a Granulation Process. Biotechnology and Bioengineering 87, 184-193, 2004. 10. Azeredo J, Henriques M, Sillankorva S, Oliveira R. Extraction of Exopolymers from Biofilms: the Protective Effect of Glutaraldhyde. Water Science and Technology 47:5, 175–179, 2003. 11. Azeredo J, Pacheco AP, Lopes I, Oliveira R, Vieira MJ. Monitoring Cell Detachment by Surfactants in a Parallel Plate Flow Chamber. Water Science and Technology 47:5, 77–82, 2003. 12. Azevedo HS, Gama FM, Reis RL, In-vitro assessment of the enzymatic degradation of several starch based biomaterials. Biomacromolecules 4, 1703-1712, 2003. 13. Azevedo N, Pacheco P, Keevil CA, Vieira MJ. Nutrient shock and incubation atmosphere influence the recovery of viable Helicobacter pylori from water. Applied and Environmental Microbiology 70:1, 490-493, 2004. 46 DEPARTMENT OF BIOLOGICAL ENGINEERING RESEARCH 14. Azevedo NF, Vieira MJ, Keevil CW. Establishment of a Continuous Model System to Study Helicobacter pylori Survival in Potable Water Biofilms. Water Science and Technology 47:5, 155–160, 2003. 15. Belo I, Pinheiro R, Mota M. Fed-batch cultivation of Saccharomyces cerevisiae in a hyperbaric bioreactor. Biotechnology Progress. 19:2, 665-671, 2003. 16. Brányik T, Vicente AA, Cruz JMM, Teixeira JA. Continuous primary beer fermentation with brewing yeast immobilized on spent grains. Journal Institute of Brewing, 108:4, 410-415, 2002. 17. Brányik T, Vicente AA, Cruz JMM, Teixeira JA. Continuous Primary Fermentation of Beer with Yeast Immobilized on Spent Grains – The Effect of Operational Conditions. Journal of the American Society of Brewing Chemists 62, 29-34, 2004. 18. Campelo AF, Belo I. Fermentative capacity of baker's yeast exposed to hyperbaric stress. Biotechnology Letters 26, 1237-1240, 2004. 19. Castro I, Gonçalves O, Teixeira JA, Vicente AA. Comparative Study of Selva and Camarosa Strawberries for the Commercial Market. Journal of Food Science 67, 2132-2137, 2002. 20. Castro I, Teixeira JA, Salengke S, Sastry SK, Vicente AA. Ohmic heating of strawberry products: electrical conductivity measurements and degradation kinetics. Innovative Food Science and Emerging Technologies 5, 27-36, 2004. 21. Castro I, Teixeira JA, Vicente AA. The influence of field strength, sugar and solid content on electrical conductivity of strawberry products. Journal of Food Process Engineering 26:1, 17-30, 2003. 22. Castro I, Teixeira JA, Salengke S, Sastry SK, Vicente AA. Ohmic heating of strawberry products: electrical conductivity measurements and degradation kinetics. Innovative Food Science and Emerging Technologies 5, 27-36, 2004. 23. Cerca N, Pier GB, Vilanova M, Oliveira R, Azeredo J. Influence of batch or fed-batch growth on Staphylococcus epidermidis biofilm formation. Letters in Applied Microbiology 39, 420-424, 2004. 24. Cerca N, Pier G, Oliveira R, Azeredo J. Comparative evaluation of coagulase-negative staphylococci (CoNS) adherence to acrylic by a static method and a parallel-plate flow dynamic method. Research in Microbiology 55:9, 755-760, 2004. 25. Coelho M, Azevedo D, Teixeira JA, Rodrigues AE. Dextran and fructose separation on a SMB continuous chromatographic unit, Biochemical Engineering Journal 12:3, 215-221, 2002. 26. Coelho MAZ, Belo I, Pinheiro R, Amaral AL, Mota M, Coutinho JAP, Ferreira EC. Effect of hyperbaric stress on yeast morphology: study by automated image analysis. Applied Microbiology and Biotechnology 66, 318-324, 2004. 27. Crispim A, Mota M. Leather shavings treatment – An enzymatic approach source. Journal of the Society of Leather Technologists and Chemists 87:5, 203-207, 2003. 28. Crispim A, Mota M. Unhairing with enzymes. Journal of the Society of Leather Technologists and Chemists 87:5, 198-202, 2003. 29. Dias N, Amaral A, Ferreira EC, Lima N. Automated image analysis to improved bead ingestion toxicity test counts in the protozoan Tetrahymena pyriformis. Letters in Applied Microbiology 37:3, 230-233, 2003. 30. Dias N, Lima N. A comparative study using a fluorescence-based and a direct- count assays to determine cytotoxicity in Tetrahymena pyriformis. Research Microbiology 153, 313-322, 2002. 31. Dias N, Mortara RA, Lima N. Morphological and physiological changes in Tetrahymena pyriformis for the in vitro cytotoxicity assessment of Triton X- 100. Toxicology in Vitro 17:3, 357–366, 2003. 47 DEPARTMENT OF BIOLOGICAL ENGINEERING RESEARCH 32. Dias R, Teixeira JA, Mota M, Yelshin A. Preparation of controlled particulate mixtures with Glass Beads of different Sizes. Separation and Purification Technology 37, 69-80, 2004. 33. Dias RP, Teixeira JA, Mota M, Yelshin AI. Particulate Binary Mixtures: Dependence of Packing Porosity on Particle Size Ratio. Industrial Engineering & Chemistry Research 43, 7912-7919, 2004. 34. Domingues L, Lima N, Teixeira JA. Aspergillus niger β-galactosidase production in a continuous high yeast cell density reactor. Process Biochemistry 40: 1151-1154, 2005. 35. Domingues L, Oliveira C, Castro I, Lima N,Teixeira JA. Production of β-galactosidase from recombinant Saccharomyces cerevisiae grown on lactose. Journal of Chemical Technology and Biotechnology 79, 809815, 2004. 36. Domingues L, Teixeira JA, Pentillä M, Lima N. Construction of flocculent Saccharomyces cerevisiae strain secreting high levels of Aspergillus niger β-galactosidase. Applied Microbiology and Biotechnology 58:5, 645-650, 2002. 37. Dourado F, Barros A, Mota M, Coimbra MA, Gama FM. Anatomy and cell wall polysaccharides of almond (Prunus dulcis D.A. Webb) seeds. Journal of Agricultural and Food Chemistry 52:5, 1364-1370, 2004. 38. Dourado F, Bastos M, Mota M, Gama FM. Studies on the properties of Celluclast/Eudragit L-100 conjugates. Journal of Biotechnology 99:2, 121-131, 2002. 39. Dourado F, Vasco P, Barros A, Mota M, Coimbra MA, Gama FM. Characterisation of Chilean hazelnut (Gevuina avellana) tissues: light microscopy and cell wall polysaccharides. Journal of Science of Food and Agriculture 83:3, 158-165, 2003. 40. Dourado F, Madureira P, Carvalho V, Coimbra MA, Vilanova M, Mota M, Gama FM. Purification, structure and immunobiological activity of an arabinan-rich pectic polysaccharide from cell walls of Prunus dulcis seeds. Carbohydrate Research 339, 2555-66, 2004. 41. Ferraz AI, Tavares T, Teixeira JA. Cr(III) Removal and Recovery from Saccharomyces cerevisiae. Chemical Engineering Journal 105, 11-20, 2004. 42. Ferreira EC, Lima R, Salcedo R. Spreadsheets in Chemical Engineering Education - A Tool in Process Design and Integration. International Journal of Engineering Education 20, 928-938, 2004. 43. Garcia-López L, Veiga MC, Nogueira R, Aparicio A, Melo LF. A technique using a membrane flow cell to determine average mass transfer coefficients and tortuosity factors in biofilms. Water Science and Technology 47:5, 61–67, 2003. 44. Giao MS, Montenegro MI, Vieira MJ, Monitoring biofilm formation by using cyclic voltammetry - effect of the experimental conditions on biofilm removal and activity. Water Science and Technology 47:5, 51–56, 2003. 45. Guimarães C, Matos C, Azeredo J, Mota M, Oliveira R. The Importance of the Morphology and Hydrophobicity of Different Carriers on the Immobilization and Sugar Refinery Effluent Degradation Activity of Phanerochaete chrysosporium. Biotechnology Letters 24:10, 795-800, 2002. 46. Guimarães C, Porto P, Oliveira R, Mota M. Continuous decolorization of a melanoidin containing wastewater from sugar refinery in a modified rotating biological contactor with Phanerochaete chrysosporium immobilized on polyurethane foam disks. Process Biochemistry 40, 535-540, 2005. 47. Henriques M, Azeredo J, Oliveira R. Adhesion of Candida albicans and Candida dubliniensis to acrylic and hydroxyapatite. Colloids and Surfaces B: Interfaces 33, 235-241, 2004. 48 DEPARTMENT OF BIOLOGICAL ENGINEERING 48. RESEARCH Henriques M, Gasparetto K, Azeredo J, Oliveira R. Experimental Methodology to Quantify Candida albicans cell surface hydrophobicity. Biotechnology Letters 24, 1111-1115, 2002. 49. Kawasse FM, Amaral PF, Rocha-Leão MHM, Coelho MAZ, Amaral AL, Ferreira EC. Morphological Analysis of Yarrowia lipolytica under Stress Conditions through Image Processing. Bioprocess and Biosystems Engineering 25:6, 371-375, 2003. 50. Klein J, Vicente AA, Teixeira JA. Hydrodynamic considerations on optimal design of a three-phase airlift bioreactor with high solids loading. Journal of Chemical Technology and Biotechnology 78, 935-944, 2003. 51. Klein J, Vicente AA, Teixeira JA. Hydrodynamics of a three-phase airlift reactor with an enlarged dual separator - application to high cell density systems. Canadian Journal of Chemical Engineering 81:3-4, 433-443, 2003. 52. Lemos MA, Teixeira JA, Domingues MRM, Mota M, Gama FM. The enhancement of the cellulolytic activity of Cellobiohydrolase I and Endoglucanase by the addition of Cellulose Binding Domains derived from Trichoderma reesei. Enzyme and Microbial Technology 32:1, 35-40, 2003. 53. Lubenova V, Rocha I, Ferreira EC. Estimation of Multiple Biomass Growth Rates and Biomass Concentration in a Class of Bioprocesses. Bioprocess and Biosystems Engineering 25:6, 395-406, 2003. 54. Marques S, Pala H, Alves L, Amaral-Collaço MT, Gama FM, Gírio FM. Characterisation and application of glycanases secreted by Aspergillus terreus CCMI 498 and Trichoderma viride CCMI 84 for mixed office wastepaper enzymatic dinking. Journal of Biotechnology 100:3, 209 - 219, 2002. 55. Martins MAM, Lima N, Silvestre AJD, Queiroz MJ. Comparative studies of fungal degradation of single or mixed bioaccessible reactive azo dyes. Chemosphere 52, 967-973, 2003. 56. Martins MAM, Queiroz MJ, Silvestre AJD, Lima N. Relationship of chemical structures of textile dyes on the pre-adaptation medium and the potentialities of their biodegradation by Phanerochaete chrysosporium. Research Microbiology 153, 361-368, 2002. 57. Mota M, Teixeira JA, Bowen WR, Yelshin A. Interference of Coarse and Fine Particles of Different Shape in Mixed Porous Beds and Filter Cakes, Minerals Engineering 16:2, 135-144, 2003. 58. Mota M, Teixeira JA, Keating JB, Yelshin A. Changes in diffusion through the brain extra cellular space. Biotechnology and Applied Biochemistry 39:2, 223-232, 2004. 59. Mota M, Teixeira JA, Yelshin A. Immobilized particles in Gel Matrix-Type Porous Media. Non-homogeneous Cell Distribution. Biotechnology Progress 18:4, 807-814, 2002. 60. Mota M, Teixeira JA, Yelshin A. Influence of cell-shape on the cake resistance in dead-end cross-flow filtration. Separation and Purification Technology 27:2, 137-144, 2002. 61. Moure A, Dourado F, Sineiro J, Gama FM, Domínguez H. Physico-chemical, functional and structural characterization of fiber from defatted Rosa rubiginosa and Gevuina avellana seeds. Journal of the Science of Food and Agriculture 84,1951-1959,2004. 62. Nakari-Setala T, Azeredo J, Henriques M, Oliveira R, Teixeira J, Linder M, Penttilä M. Expression of a fungal hyfrophobin in Saccharomyces cell wall: Effect on cell surface Properties and immobilisation. Applied and Environmental Microbiology 68:7, 3385-3391, 2002. 63. Neves L, Oliveira R, Alves MM. Influence of inoculum activity on the bio-methanization of a kitchen waste under different waste/inoculum ratios. Process Biochemistry 39, 2019-2024, 2004. 49 DEPARTMENT OF BIOLOGICAL ENGINEERING RESEARCH 64. Nicolau A, Mota M, Lima N. Effect of different toxic compounds on ATP content and acid phosphatase activity in axenic cultures of Tetrahymena pyriformis. Ecotoxicology and Environmental Safety 57, 129135, 2004. 65. Nigmatullin R, Lovitt R, Wright C, Linder M, Nakari-Setälä T, Gama M. Atomic force microscopy study of cellulose surface interaction controlled by cellulose binding domains. Colloid and Surfaces B: Biointerfaces 35, 125-135, 2004. 66. Nogueira R, Melo LF, Purkhold U, Wuertz S, Wagner M. Microbial population dynamics versus nitrification performance in biofilm reactors: Effects of hydraulic retention time and the presence of organic carbon. Water Research 36:2, 469-481, 2002. 67. Oliveira JM, Araújo IM, Pereira OM, Maia JS, Amaral AJ, Maia MO. Characterization and differentiation of five Vinhos Verdes grape varieties by monoterpenic compounds. Analytica Chimica Acta 513, 269-275, 2004. 68. Oliveira R, Ferreira EC, Azevedo SF. Stability, Dynamics of Convergence and Tuning of Observer-based Kinetics Estimators. Journal of Process Control 12:2, 311-323, 2002. 69. Pala H, Mota M, Gama FM. Enzymatic modification of paper fibres. Biocatalysis and Biotransformation 20:5, 353-361, 2002. 70. Pala H, Mota M, Gama FM. Enzymatic versus chemical deinking of non-impact ink printed wastepaper. Journal of Biotechnology 108:1, 79-89, 2004. 71. Paterson RMR, Venâncio A, Lima N. Solutions to Penicillium taxonomy crucial to mycotoxin research and health. Research in Microbiology 155, 507-513, 2004. 72. Pereira M, Wu Y-T, Madeira P, Venâncio A, Macedo E, Teixeira JA. Liquid-Liquid Equilibrium Phase Diagrams of New Aqueous Two-Phase Systems: Ucon 50-HB5100 + Ammonium Sulfate + Water, Ucon 50-HB5100 + Polyvinyl Alcohol + Water, Ucon 50-HB5100 + Hydroxypropyl Starch + Water and Polyethylene Glycol 8000 + Polyvinyl Alcohol + Water. Chemical Engineering Data 48, 43-47, 2004. 73. Pereira M, Wu Y-T, Venâncio A, Teixeira JA. Aqueous Two-Phase Extraction Using Thermoseparating Polymer: A New System for the Separation of Endo-polygalacturonase. Biochemical Engineering Journal 15:2, 131-138, 2003. 74. Pereira MA, Cavaleiro AJ, Mota M, Alves MM. Accumulation of LCFA onto anaerobic sludge under steady state and shock loading conditions: effect on acetogenic and methanogenic activity. Water Science and Technology 48:6, 33-40, 2003. 75. Pereira MA, Mota M, Alves MM. Operation of an anaerobic filter and an EGSB reactor for the treatment of oleic-acid based effluents: influence of inoculum quality. Process Biochemistry 37:9, 1025-1031, 2002. 76. Pereira MA, Pires OC, Mota M, Alves MM. Anaerobic degradation of oleic acid by suspended and granular sludge: identification of palmitate as a key intermediate. Water Science and Technology 45:10, 139-144, 2002. 77. Pereira MA, Roest K, Stams AJM, Akkermans ADL, Amaral AL, Pons M-N, Ferreira EC. Mota M, Alves MM. Image analysis, methanogenic activity measurements and molecular biological techniques to monitor granular sludge from an EGSB reactor fed with oleic acid. Water Science and Technology 47:5, 181-188, 2003. 78. Pereira MA, Roest K, Stams AJM, Mota M, Alves MM, Akkermans A. Molecular monitoring of microbial diversity in expanded granular sludge bed (EGSB) reactors treating oleic acid. FEMS Microbiology Ecology 41:2, 95-103, 2002. 50 DEPARTMENT OF BIOLOGICAL ENGINEERING 79. RESEARCH Pereira MO, Kuehn M, Wuertz S, Neu T, Melo L. Effect of flow regime on the architecture of a Pseudomonas fluorescens biofilm. Biotechnology and Bioengineering 78:2, 164-171, 2002. 80. Pereira MO, Morin P, Vieira MJ, Melo LF. A versatile reactor for continuous monitoring of biofilm properties in laboratory and industrial conditions. Letters in Applied Microbiology 34:1, 22-26, 2002. 81. Pereira MO, Vieira MJ, Melo LF. The role of kaolin particles in the performance of a carbamate-based biocide for water bacterial control. Water Environment Research 74:3, 235-241, 2002. 82. Pereira M A, Sousa DZ, Mota M, Alves MM. Mineralization of LCFA associated to anaerobic sludge: kinetics, transport limitations, enhancement of methanogenic activity and effect of VFA. Biotechnology and Bioengineering 88, 502-511, 2004. 83. Pinheiro R, Belo I, Mota M. Growth and β-galactosidase activity in cultures of Kluyveromyces marxianus under increased air pressure. Letters in Applied Microbiology 37:6, 438-442, 2003. 84. Pinheiro R, Belo I, Mota M. Oxidative stress response of Kluyveromyces marxianus to hydrogen peroxide, paraquat and pressure. Applied Microbiology and Biotechnology 58:6, 842-847, 2002. 85. Pinto R, Moreira S, Mota M, Gama FM. Cellulose-Binding Domains Adsorption to Cellulose. Langmuir 20, 1409-1413, 2004. 86. Quintelas C, Tavares T. Lead(II) and Iron(II) removal from aqueous solution: biosorption by a bacterial biofilm supported on granular activated carbon. Journal of Resource and Environmental Biotechnology 3, 196-202, 2002. 87. Reis N, Vicente AA, Teixeira JA, Mackley MR. Residence times and mixing of a novel continuous oscillatory flow screening reactor. Chemical Engineering Science, 59, 4967-4974, 2004. 88. Rocha I, Ferreira EC. On-line simultaneous monitoring of glucose and acetate with FIA during high cell density fermentation of recombinant E. coli. Analytica Chimica Acta 462:2, 293-304, 2002. 89. Rodrigues AC, Wuertz S, Brito AG, Melo LF. Three-dimensional distribution of GFP-tagged Pseudomonas putida during biofilm formation on solid PAHs assessed by confocal laser scanning microscopy. Water Science and Technology 47:5, 139-142, 2003. 90. Rodrigues LR, Venâncio A, Teixeira JA. Recovery of the Proteose Peptone Component 3 from Cheese Whey in Reppal PES 100 / Polyethylene Glycol Aqueous Two-Phase Systems. Biotechnology Letters 25, 651-655, 2003. 91. Rodrigues LR, van der Mei HC, Teixeira JA, Oliveira R. Influence of biosurfactants from probiotic bacteria on formation of biofilms on voice prostheses. Applied and Environmental Microbiology 70, 4408-4410, 2004. 92. Rodrigues LR, van der Mei HC, Teixeira JA, Oliveira R. Biosurfactant from Lactococcus lactis 53 inhibits microbial adhesion on silicone rubber. Applied Microbiology and Biotechnology 66, 306–311, 2004. 93. Rusicka MC, Drahos J, Mena P, Teixeira JA. Effect of viscosity on homogeneous-heterogeneous regime transition in bubble columns. Chemical Engineering Journal 96:1-3, 15-22, 2003. 94. Santos IM, Abrunhosa L, Venâncio A, Lima N. The effect of preservation techniques on patulin and citrinin production by Penicillium expansum Link. Letters in Applied Microbiology 35:4, 272-275, 2002. 95. Serra R, Abrunhosa L, Kozakiewicz Z, Venâncio A, Lima N. Use of ozone to reduce molds in a cheese ripening room. Journal of Food Protection 66, 2355-2358, 2003. 51 DEPARTMENT OF BIOLOGICAL ENGINEERING RESEARCH 96. Serra R, Abrunhosa L, Kozakiewicz Z, Venâncio A. Black Aspergillus species as ochratoxin A producers in Portuguese wine grapes. International Journal of Food Microbiology 88, 63-68, 2003. 97. Serra R, Mendonça C, Abrunhosa L, Pietri A, Venâncio A. Determination of ochratoxin A in wine grapes: comparison of extraction procedures and method validation. Analytica Chimica Acta 513, 41-47, 2004. 98. Sillankorva S, Oliveira R, Vieira MJ, Sutherland IW, Azeredo J. Bacteriophage F S1 Infection of Pseudomonas fluorescens Planktonic cells versus Biofilms. Biofouling 20, 133-138, 2004. 99. Sillankorva S, Sutherland IW, Vieira MJ, Oliveira R, Azeredo J. Pseudomonas fluorescens infection by bacteriophage phi S1 – the influence of temperature, host growth phase and media. FEMS Microbiology Letters 241, 13-20, 2004. 100. Simões M, Carvalho H, Pereira MO, Vieira MJ. Studies on the behaviour of Pseudomonas fluorescens biofilms after ortho-phthaldehyde treatment. Biofouling 19:3, 151-157, 2003. 101. Simões M, Pereira MO, Vieira MJ. Effect of different concentrations of ortho-phthalaldehyde on biofilms formed by Pseudomonas fluorescens under different flow conditions. Biofouling 19:5, 287-295, 2003. 102. Simões M, Pereira MO, Vieira MJ. Monitoring the Effects of Biocide Treatment of Pseudomonas fluorescens Biofilms Formed under Different Flow Regimes. Water Science and Technology 47:5, 217223, 2003. 103. Soares EV, Vroman A, Mortier J, Mota M. Carbohydrate Carbon Sources Induce Loss of Flocculation of an Ale Brewing Yeast Strain. Journal of Applied Microbiology 96, 117-1123, 2004. 104. Teixeira P, Lopes Z, Azeredo J, Oliveira R, Vieira MJ. Physico-chemical surface characterization of a bacterial population isolated from a milking machine. Food Microbiology 22, 247-251, 2005. 105. Teixeira P, Oliveira R. Metabolism of Alcaligenes denitrificans in biofilm vs planktonic cells. Journal of Applied Microbiology 92:2, 256-260, 2002. 106. Vieira MJ, Pinho IA, Gião MS, Montenegro MI. The Use of Cyclic Voltammetry to detect biofilms formed by Pseudomonas fluorescens on Platinum surfaces. Biofouling 19:4, 215-222, 2003. 107. Vilanova M, Teixeira L, Caramalho I, Torrado E, Marques A, Madureira P, Ribeiro A, Ferreira P, Gama FM, Demengeot J. Immune protection against systemic candidiasis in mice immunized with Secreted Aspartic Proteinase. Immunology 111,334-342, 2004. 108. Wagner M, Loy A, Nogueira R, Purkhold U, Lee N, Daims H. Microbial community composition and function in wastewater treatment plants. Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology 81(1-4), 665-680, 2002. 109. Xu X, Madeira PP, Teixeira JA, Macedo EA. A New Modified Wilson Equation for the Calculation of VaporLiquid Equilibrium of Aqueous Polymer Solutions, Fluid Phase Equilibria 213, 53-63, 2003. 110. Yelshin A, Teixeira JA, Bowen WR, Mota M. Role of Fractional Cake Composition in Cake Resistance. Transactions of the Filtration Society 2:2, 45-48, 2002. 52 DEPARTMENT OF BIOLOGICAL ENGINEERING 7. The "Micoteca da Universidade do Minho" The Micoteca da Universidade do Minho - MUM - is a fungi culture collection established in 1996, with the double purpose of maintaining and supplying strains for research on biotechnology and for use in teaching laboratories, and also to act as a centre of expertise, information and training. The collection is registered with BioCise (Biological Collection Information Service in Europe) and with the World Data Centre of Microorganisms (WDCM). The collection is member of European Culture Collections’Organisation (ECCO) and World Federation of Culture Collectons (WFCC). MUM is a board member of ECCO. The collection kept in the Department of Biological Engineering at the University of Minho holds about 150 identified species. The holdings include recognized standard strains and isolates derived from research activities, mainly of the genera Penicillium and Aspergillus. Details of the open collection’s holdings are available on-line at www.micoteca.deb.uminho.pt. Research activities are presently focused on the subjects of biodegradation and biodeterioration, studies of mycotoxins in food products and studies of air quality in industrial environments. The available specialized books include around 500 titles of which 250 are monographs. Training services include individual training programmes to fulfil specialised or confidential training needs, in addition to advanced courses. A course on food-borne fungi takes place every year, aimed at professionals working in microbiology laboratorys in the food and beverage industry and researchers in microbiology. It provides an introduction to the identification of important groups of fungal contaminants of foods and beverages. A manual on food-borne fungi was prepared to support this course. Post-graduation studies (MSc, PhD) in mycology related areas are also available.