Sociedade Brasileira de Espectrometria de Massas – BrMASS
5º Congresso BrMass – 07 a 11 de Dezembro de 2013
PRO – PROTEINS & PEPTIDES- Poster Presentation
Use of proteomics techniques in the study of Wistar rats proteins
expression upon exposure to 2,3 butanedione
Leticia D. L. Jedlicka1*, Sheila Guterres1, Bernadete de Faria1 ,Richard G.
Landgraf1, Etelvino J. H. Bechara1, 2, Nilson A. Assunção1
[email protected]
1
Instituto de Ciências Ambientais, Químicas e Farmacêuticas, Universidade
Federal de São Paulo, São Paulo, Brasil.
2
Instituto de Química, Universidade de São Paulo, São Paulo, Brasil.
The 2,3 butanedione attribute butter flavor to products, this compound is largely
utilized by food industry mainly for products such as microwave popcorn, buttered
cookies beyond occur in ferment food as wine, dairy products and others,[1] recently
this additive is relationed with lung diseases[2]. Peroxynitrite is a powerful nucleophile
originated in organisms by the diffusion-controlled reaction of nitric oxide (NO•) with
the superoxide ion radical (O2•-), formation of peroxynitrite is increased in proinflammatory conditions implicating in several diseases [3]. Together 2,3 butanedione
and peroxynitrite in physiological conditions produce highly reactive radical species and
these can cause important structural and functional alterations in proteins[4]. Studies In
vitro conducted by our group showed that acetyl radicals can be produced in a reaction
medium buffer with 2,3 butanedione and peroxynitrite, this radicals are able to form
stable covalent bonds with amino acid, peptide and protein adducts the double
acetylation of lysine, demonstrating the high reactivity of these compounds when in
contact with nitrogen-containing biomolecules found in biological systems. Our results
support the hypothesis that, unlike inflammatory sites, post-translational protein
modification by radical acetylation did not only occur at the protein N-terminus of cells
under nitrosative and carbonyl stress, double acetylation may also occur at the Nterminus of lysine residues [5]. In this present study we compare the protein expression
in animals in state of oxidative stress induced by lung infection and treated with 2,3
butanedione with helthy animals treated with saline vehicle . The data presented in this
study showed changes in protein expression profile related to 2,3 butanedione in both
states studied (normal and sepsis) and stimulated the process of oxidative stress with
increased production of peroxynitrite. The results obtained even now show an effect on
the change in lung and liver proteins expression indicating that the 2,3 butanedione
has a negative effect both in a healthy organism and an organism also subjected to an
oxidative stress condition.
References
[1] Bartowsky, E. J., and Henschke, P. A. 2004 Int. J. Food Microbiol.96, 235–252.
[2] Harber, P., Saechao, K., Boomus, C. 2006 Toxicol. Rev. 25, 261–272.
[3] Radi, R., Cassina, A., Hodara, R., Quijano, C., Castro, L. 2002 Free Radic Biol
Med33,1451–1464.
[4] Massari, J., Fujiy, D.E., Dutra, F., Vaz, S.M., Costa, A.C., 2011 J. Am. Chem.
Soc., 133, 20761-20768
[5] Alves, A. N. L., Jedlicka,L.D.L., Massari,J., Juliano, M.A., Bechara, E. J.H.,
Assunção, N.A 2013 J. Braz. Chem. Soc., In Press 1-8.
5º Congresso BrMass – 07 a 11 de Dezembro de 2013