CURRICULUM VITAE Joana Andrêa Soares Amaral January 2013 1. Biographic Data Name: Joana Andrêa Soares Amaral Filiation: João do Espírito Santo Amaral e Maria Helena Soares Amaral Birth date: 16 de Setembro de 1976 Birth place: Freguesia de Santa Maria, concelho de Viseu, Distrito de Viseu Nationality: Portuguesa Address: Viso Sul Lote 92 – A, 3510 Viseu Telephone: 966047512 E-mail: [email protected]; [email protected] 2. Academic degrees Licentiate degree in Pharmaceutical Sciences, in the Pharmacy Faculty of the Porto University, in July 2000, with the final grade of 16 (sixteen values). PhD degree in Nutrition and Food Chemistry by the Pharmacy Faculty of the Porto University, in September 2006. 3. Profissional Activity 4.1. Teaching activity From 2010 to present, is an adjunct Professor at the Polytechnic Institute of Bragança, teaching the classes of: - Pharmacognosy (theoric), Phytopharmacy and Aromatic and Medicinal Plants degree - Pharmaceutical Technology and Cosmetics(theoric), Phytopharmacy and Aromatic and Medicinal Plants degree - Organic Chemistry I (theoric), Chemical and Biological Engineering degree - Organic Chemistry II (theoric and laboratorial), Chemical and Biological Engineering degree - Biology I (theoric and laboratorial), Chemical and Biological Engineering degree - Biology II (theoric), Chemical and Biological Engineering degree - Molecular and Celular Biology (theoric and laboratorial), Biomedical Engineering degree - Microbiology (theoric), Biomedical Engineering degree 2 From 2005 to 2010, was a 2nd triennial assistant teacher at the Polytechnic Institute of Bragança, teaching the classes of: - Chemistry (theoretic-practical), Civil Engineering degree - General Chemistry I (theoric and theoretic-pratical), Chemical Engineering degree - General Chemistry II (theoric and theoretic-pratical), Chemical Engineering degree - Organic Chemistry (theoric and theoretic-practical), Chemical Engineering degree - Organic Chemistry I (theoric and theoretic-practical), Chemical and Biological Engineering degree - Organic Chemistry II (theoric and laboratorial), Chemical and Biological Engineering degree - Biology I (theoric and laboratorial), Chemical and Biological Engineering degree - Biology II (laboratorial), Chemical and Biological Engineering degree - Organic Chemistry (theoric and theoretic-practical), Biomedical Engineering degree - Biology I (theoric and laboratorial), Chemistry Engineering degree - Biochemistry (theoric and theoretic-practical), Biomedical Engineering degree - Technology of Natural Products (theoric and laboratorial), Phytochemistry and Phytopharmacology degree - General Microbiology (theoric and laboratorial), Technologic Specialization Course (CET) in Biological and Chemical Analysis. - Environmental Chemistry and Microbiology (Microbiology part, theoric and laboratorial), Technologic Specialization Course (CET) in Biological and Chemical Analysis. - Molecular and Celular Biology (theoric and laboratorial), Biomedical Engineering degree - Microbiology (theoric and theoretic-practical), Biomedical Engineering degree From September 2002 until September 2005, had a license from the European program PRODEP III, in order to obtain the PhD degree. In 2001-2002, was a 1st triennial assistant teacher at the Polytechnic Institute of Bragança, teaching the classes of General Chemistry I and the classes of Organic Chemistry of the Chemical Engineering degree. In 2000-2001 was an invited assistant teacher of the Polytechnic Institute of Bragança, teaching the classes of Chemistry (Civil Engineering and Industrial Engineering and Management degrees), and the classes of Organic Chemistry (Chemical Engineering). 4.2. Supervision Experience PhD students 2012/present Co-supervisor of Sónia Eduarda Ferrás Soares, PhD student in Sustainable Chemistry from REQUIMTE/University of Porto, with the thesis “Authenticity and traceability of Portuguese PDO honeys: application of metabolomics andy biomolecular techniques” (FCT Grant SFRH/BD/75091/2010) 3 Master students: 2012-present Co-supervisor of the student Jorge Miguel Ferreira Delgado, MSc student of the Master in Biomedical Technology of the Polythechnic Institute of Bragança, with the thesis “Preparation and characterization of nanocarriers (Nanocapsules, Nanospheres, Nanostructured lipid carriers and Liposomes) formulated without active substance” 2012-present Co-supervisor of the student Angela Daniela Alves da Costa, MSc student of the Master in Biomedical Technology of the Polythechnic Institute of Bragança, with the thesis “Cytotoxicity evaluation of different nanocarriers without active substance” 2012-present Co-supervisor of the student Carla Peixoto, MSc student of the Master in Biomedical Technology of the Polythechnic Institute of Bragança, with the thesis “Development of fast methodologies for the quantification of cysteine/cystine” 2011-2012 Co-supervisor of Raquel Oliveira Rodrigues, MSc student of the Master in Biomedical Technology of the Polythechnic Institute of Bragança, with the thesis “Development and optimization of a biological protocol for DNA detection of Escherichia coli O157:H7 by Quartz Crystal Microbalance with Dissipation (QCM-D)” 2011-2012 Co-supervisor of Sofia Alexandra de Jesus Alves dos Santos, MSc student of the Master in Biotechnology and Food Quality of the Trás-os-Montes and Alto Douro University, with the thesis “Determination of the in vitro antimicrobial activity of essential oils from aromatic plants used in gastronomy against foodborne pathogenic bacteria” 2009-2010 Co-supervisor of Manuel Martinho Vilas Boas Sepúlveda, MSc student of the Master in Chemical Engineering of the Polythechnic Institute of Bragança, with the thesis “Biodegradation of Polyurethanes from renewable sources” 2008-2009 Supervisor of Fátima Susana Claro, MSc student of the Master in Chemical Engineering of the Polythechnic Institute of Bragança, with the thesis “Development of an analytical methodology for the determination of biogenic amines in meat sausages” 2007-2008 Supervisor of Hélder Manuel Marçal de Oliveira, MSc student of the Master in Chemical Engineering of the Polythechnic Institute of Bragança, with the thesis “Professional Internship at INOVPLASTIKA” 2007-2008 Supervisor of Sónia Eduarda Ferráz Soares, MSc student of the Master in Chemical Engineering of the Polythechnic Institute of Bragança, with the thesis “Authenticity evaluation of meat sausages commercialized in Portugal” Graduate students 2011-2012 Co-supervisor of the students Melanie Pereira and Silvia Ribeiro, BSc students of the Biomedical Engineering of the Polythechnic Institute of Bragança, with 4 the thesis “Antimicrobial activity evaluation of dental elixirs” 2011-2012 Co-supervisor of the students Andreia Ribeiro and Dalila Vieira, BSc students in Biomedical Engineering of the Polythechnic Institute of Bragança, with the thesis “Microencapsulation of Vitamin E for medical and food purposes” 2010-2011 Co-supervisor of the students Erica Leite and Diana Vilas-Boas, BSc students in Biomedical Engineering of the Polythechnic Institute of Bragança, with the thesis “Chitosan-based films enriched with essential oils for biomedical applications” 2009-2010 Co-supervisor of the students Joana Catarina Pires Lopes and Rui Gonçalo Rosa, BSc students of the Biomedical Engineering of the Polythechnic Institute of Bragança, with the thesis “Determination of β-lactamases in medical isolates of Escherichia coli 2009-2010 Co-supervisor of the students Carla Sofia da Silva Ferreira, BSc student of the Biomedical Engineering of the Polythechnic Institute of Bragança, with the thesis “Synergism of Propolis and cefoxitin in clinical isolates of Staphylococcus aureus” 2008-2009 Co-supervisor of the students Marlene Machado, BSc student of the Biomedical Engineering of the Polythechnic Institute of Bragança, with the thesis “Development of chitisan-based medical dressings for biomedical applications” 4.3. Collaboration with other institutions Since 2001, collaborates in the practical classes of the Course of Authenticity of the Master in Quality Control of the Faculty of Pharmacy of the University of Porto. 5. Scientific Activities 5.1. Publications 5.1.1. Book Edition 1. Current topics on food authentication, M.B.P.P. Oliveira, I. Mafra, J.S. Amaral (Eds), Transworld Research Network, Kerala, India, 2011. 5.1.2. Book chapters 1. S.C. Cunha, J.S. Amaral, M.B.P.P. Oliveira, Authentication of vegetable oils. In Current topics on food authentication, M.B.P.P., Oliveira, I., Mafra, J.S. Amaral, (Eds), Transworld Research Network, Kerala, India, 2011, pp. 97-128. 2. M.C. Barros, I.P. Fernandes, V. Pinto, M.J. Ferreira, M.F. Barreiro, J.S. Amaral, Chitosan as an antimicrobial agent for footwear leather components. In Biodegradable Polymers and Sustainable Composites (BIOPOL-2009), A. Jimenez & G.E. Zaikov (Eds.), Nova Publishers Inc., NY, USA, 2011, pp. 151-166. 5 3. I. Mafra, J.S. Amaral, M.B.P.P. Oliveira, Polycyclic aromatic hydrocarbons (PAH) in olive oils and other vegetable oils potential for carcinogenesis. In Olives and Olive Oil in Health and Disease Prevention, V.P. Preedy, R.R. Watson (Eds.), Elsevier, London, 489-497, 2010. 4. J.S. Amaral, I. Mafra, M.B.P.P. Oliveira, Characterization of three Portuguese varietal olive oils based on fatty acids, triacylglycerols, phytosterols and Vitamin E profiles: application of chemometrics. In Olives and Olive Oil in Health and Disease Prevention, V.P. Preedy, R.R. Watson (Eds.), Elsevier, London, 581-589, 2010. 5. S. Casal, J.S. Amaral, M.B.P.P. Oliveira, Effects of food thermal processing on vitamin E contents. In Vitamin E: New Research, M.H. Braunstein (Ed.), Nova Science Publishers, 2006, pp. 39-67. 6. G. Igrejas, J.S. Amaral, P.J.S. Rodrigues, Fall Detection Systems to be Used by Elderly People, In: Handbook of Research on ICT for Healthcare and Social Services, M.M. CruzCunha, I.M. Miranda, P. Gonçalves (Eds.), IGI Global, 449-473, 2013. 5.1.3. Publications in international journals (ISI Web of Knowledge) 1. F.M. Areias, P. Valentão, P.B. Andrade, M.M. Moreira, J. Amaral, R. Seabra, HPLC/DAD analysis of phenolic compounds from Lavender and its application to quality control. Journal of Liquid Chromatography and Related Technologies 2000, 23, 2563-2572. 2. P. Valentão, F. Areias, J. Amaral, P. Andrade, R. Seabra, Tetraoxygenated Xanthones from Centaurium erythraea. Natural Product Letters 2000, 14, 319-323. 3. J.S. Amaral, S. Casal, J.A. Pereira, R.M. Seabra, B.P.P. Oliveira, Determination of sterol and fatty acid compositions, oxidative stability and nutritional value of six walnut (Juglans regia L.) cultivars grown in Portugal. Journal of Agricultural and Food Chemistry 2003, 51, 7698-7702. 4. J.S. Amaral, R.M. Seabra, P.B. Andrade, P. Valentão, J.A. Pereira, F. Ferreres, Phenolic profile in the quality control of walnut (Juglans regia L.) leaves. Food Chemistry 2004, 88, 373379. 5. J.S. Amaral, S.C. Cunha, M.R. Alves, J.A. Pereira, R.M. Seabra, B.P.P. Oliveira, Triacylglycerols composition of walnut (Juglans regia L.) cultivars: characterization by HPLC/ELSD and chemometrics. Journal of Agricultural and Food Chemistry 2004, 52, 79647969. 6. J.S. Amaral, F. Ferreres, P.B. Andrade, P. Valentão, C. Pinheiro, A. Santos, R.M. Seabra, Phenolic profile of hazelnut (Corylus avellana L.) leaves cultivars. Natural Product Research 2005, 19, 157-163. 7. J.S. Amaral, Casal, S.; Seabra, R.M.; Oliveira, B.P.P. Development and evaluation of a normal phase liquid chromatographic method for the determination of tocopherols and tocotrienols in walnuts. Journal of Liquid Chromatography and Related Technologies 2005, 28, 785-795. 8. J.S. Amaral, Alves, R.M.; Seabra, R.M.; Oliveira, B.P.P. Vitamin E composition of walnuts (Juglans regia L.): A 3-year comparative study of different cultivars. Journal of Agricultural and Food Chemistry 2005, 53, 5467-5472. 9. J.S. Amaral, Casal, S.; Torres, D.; Seabra, R.M.; Oliveira, B.P.P. Simultaneous determination of tocopherols and tocotrienols in hazelnuts by a normal phase liquid chromatographic method. Analytical Sciences 2005, 21, 1545-1548. 6 10. Alves, R.M., Cunha, S.C., Amaral, J.S., Pereira, J.A., Oliveira, M.B.P.P. Classification of PDO olive oils on the basis of their sterol composition by multivariate analysis. Analytica Chimica Acta 2005, 549, 166-178. 11. J.S. Amaral, Casal, S.; Citová, I.; Santos, A.; Seabra, R.M.; Oliveira, B.P.P. Characterization of several hazelnut (Corylus avellana L.) cultivars based in chemical, fatty acid and sterol composition. European Food Research and Technology 2006, 222, 274-280. 12. J.S. Amaral, Cunha, S.C.; Santos, A.; Alves, M.R.; Seabra, R.M.; Oliveira, B.P.P. Influence of cultivar and environmental conditions on the triacylglycerol profile of hazelnut (Corylus avellana L.). Journal of Agricultural and Food Chemistry 2006, 54, 449-456. 13. J.S. Amaral, Casal, S.; Seabra, R.M.; Oliveira, B.P.P. Effects of roasting on hazelnut lipids. Journal of Agricultural and Food Chemistry 2006, 54, 1315-1321. 14. J.S. Amaral, Casal, S.; Alves, M.R.; Seabra, R.M.; Oliveira, B.P.P. Tocopherols and tocotrienols content of hazelnut cultivars grown in Portugal. Journal of Agricultural and Food Chemistry 2006, 54, 1329-1336. 15. Cunha, S.C.; J.S. Amaral, Fernandes, J.O.; Oliveira, M.B.P.P. Quantification of tocopherols and tocotrienols in Portuguese olive oils using HPLC with three different detection systems. Journal of Agricultural and Food Chemistry 2006, 54, 3351-3356. 16. Alasalvar, C.; J.S. Amaral, Shahidi, F. Functional lipid characteristics of Turkish Tombul hazelnut (Corylus avellana L.). Journal of Agricultural and Food Chemistry 2006, 54, 1017710183. 17. Matos, L.C.; Cunha, S.C.; J.S. Amaral, Pereira, J.A.; Andrade, P.B.; Seabra, R.M.; Oliveira, B.P.P. Chemometric characterization of three varietal olive oils (Cvs. Cobrançosa, Madural and Verdeal Transmontana) extracted from olives with different maturation indices. Food Chemistry 2007, 102, 406-414. 18. J.S. Amaral, Valentão, P.; Andrade, P.B.; Martins, R.C.; Seabra, R.M. Do cultivar, geographical location and crop season influence phenolic profile of walnut leaves? Molecules, 2008, 13, 1321-1332. 19. Alasalvar, C.; J.S. Amaral, Gülçin, S.; Shahidi, F. Lipid characteristics and essential minerals of native Turkish hazelnut varieties (Corylus avellana L.). Food Chemistry, 2009, 113, 919-925. 20. Costa, J., Mafra, I., Amaral, J.S., Oliveira, M.B.P.P. Monitoring genetically modified soybean along the industrial soybean oil extraction and refining processes by polymerase chain reaction techniques. Food Research International, 2010, 43, 301-306. 21. Costa, J., Mafra, I., Amaral, J.S., Oliveira, M.B.P.P. Detection of genetically modified soybean DNA in refined vegetable oils. European Food Research and Technology, 2010, 230, 915-923. 22. Soares, S., Amaral, J.S., Mafra, I., Oliveira, M.B.P.P. Quantitative detection of pork’s meat by duplex PCR, Meat Science, 85: 531-536, 2010. 23. J.S. Amaral, Valentão, P.; Andrade, P.B.; Martins, R.C.; Seabra, R.M. Influence of cultivar, geographical origin and ripening on the phenolic composition of hazelnut leaves. Scientia Horticulturae, 2010, 126 (2), 306-313. 24. Soares, S., Amaral, J.S., Mafra, I., Oliveira, M.B.P.P. A PCR assay to detect trace amounts of soybean in meat sausages, International Journal of Food Science and Technology, 2010, 45, 2581-2588. 7 25. Costa, J., Amaral, J.S., Mafra, I., Oliveira, M.B.P.P. Refining of Roundup Ready soya bean oil: Effect on the fatty acid, phytosterol and tocopherol profiles. Eur. J. Lipid Sci. Technol. 2011, 113, 528–535. 26. Santos, C.G., Melo, V.S., Amaral, J.S., Estevinho, L., Oliveira, M.B.P.P. Mafra, I. Identification of hare meat by a species-specific marker of mitochondrial origin. Meat Science, 2012, 90, 836-841. doi:10.1016/j.meatsci.2011.10.018 27. Amaral, J.S., Sepúlveda, M., Cateto, C.A., Fernandes, I.P., Rodrigues, A.E., Belgacem, M.N., Barreiro, M.F. “Fungal degradation of lignin-based rigid polyurethane foams” Polymer Degradation and Stability 97, 2069-2076 (2012). doi:10.1016/j.polymdegradstab.2012.03.037 28. Amaral, J.S., Ribeiro, A., Alves, S., Gonçalves, A., Poeta, P. “Chemical composition of different oregano (Origanum vulgare L.) essential oils: relevance for the activity against foodborne and spoilage bacteria”, Chem. Listy 106, s1159 (2012). 29. Amaral, J.S., Fernandes, I.P., Pinto, V., Ferreira, M.J. Barreiro, M.F., “Chitosan-based leather functional coatings with improved antimicrobial properties”, Chem. Listy 106, s793 (2012). 30. Silva, N., Alves, S., Gonçalves, A., Amaral, J.S., Poeta, P. Antimicrobial activity of essential oils from mediterranean aromatic plants against several foodborne and spoilage bacteria. Food Science and Technology International, 26 (2013). 31. S. Soares, J.S. Amaral, M.B.P.P. Oliveira, I. Mafra, A SYBR Green real-time PCR assay to detect and quantify pork meat in processed poultry meat products, Meat Science, 94, 115-120 (2013). Doi: 10.1016/j.meatsci.2012.12.012. 32. I.P. Fernandes, J.S. Amaral, V. Pinto, M.J. Ferreira, M.F. Barreiro. Development of chitosanbased antimicrobial leather coatings. Carbohydrate Polymers, 98, 1229 (2013). 33. T.J.R. Fernandes, J.S. Amaral, M.B.P.P. Oliveira, I. Mafra. A survey on genetically modified maize in foods commercialised in Portugal, Food Control, 35, 338 (2014). 34. J.S. Amaral, S. Soares, I. Mafra, M.B.P.P. Oliveira, Accessing the variability of fatty acid profile and cholesterol content of meat sausages, Rivista Italiana delle Sostanze Grasse (in press) 35. J.S. Amaral, C.G. Santos, V.S. Melo, M.B.P.P. Oliveira, I. Mafra, Authentication of a traditional game meat sausage (Alheira) by species-specific PCR assays to detect hare, rabbit, red deer, pork and cow meats, Food Research International, (in press). 5.1.4. Proceedings (ISI Web of Knowledge) 1. G. Igrejas, J.S. Amaral, P.J. Rodrigues, M.B.P.P. Oliveira, A T-S fuzzy model based on initial FCM partition to estimate the composition of commercial blended vegetable oils. Book of Proceedings of the FOODSIM’2010 Conference, Cadavez, V. and Thiel, D. (Eds.), Eurosis-ETI, Belgium, pp. 19-22. 2. P.J. Rodrigues, J.S. Amaral, A. Santos, G. Igrejas, A segmentation approach for the identification of hazelnut cultivars. Book of Proceedings of the FOODSIM’2010 Conference, Cadavez, V. and Thiel, D. (Eds.), Eurosis-ETI, Belgium, pp. 46-52. 3. S. Soares, J.S. Amaral, I. Mafra, M.B.P.P. Oliveira, The use of model samples to estimate the level of fraudulent addition of pork meat in poultry meat by real-time PCR. Book of Proceedings 8 of the FOODSIM’2010 Conference, Cadavez, V. and Thiel, D. (Eds.), Eurosis-ETI, Belgium, 2010, pp. 171-174. 5.1.4. Other Proceedings 1. J.S. Amaral, R.M. Seabra, M.B.P.P. Oliveira, Tocopherols and tocotrienols composition of hazelnuts. Intrafood 2005, Book of Proceedings, Vol. I, Eds. Fito, P. and Toldrá, F., pp. 345 348, 2005. 2. S.C. Cunha, J.S. Amaral, J.O. Fernandes, M.B.P.P. Oliveira, Simultaneous determination of tocopherols and tocotrienols in virgin olive oils by Normal Phase HPLC coupled with DiodeArray/Fluorescence detectors. Intrafood 2005, Book of Proceedings, Vol. I, Eds. Fito, P. and Toldrá, F., pp. 353 - 356, 2005. 3. J.S. Amaral, S. Soares, I. Mafra, M.B.P.P. Oliveira, Polymerase chain reaction for the detection of allergens: the case of soybean in processed meat products. Book of Proceedings of the 9º Encontro de Química dos Alimentos, 29 April to 3 May, 2009, Angra do Heroísmo, Açores, Portugal. 4. S. Soares, J.S. Amaral, I. Mafra, M.B.P.P. Oliveira, Species identification in heat processed meat products. Book of Proceedings of the 9º Encontro de Química dos Alimentos, 29 April to 3 May, 2009, Angra do Heroísmo, Açores, Portugal. 5. I. Mafra, J.S. Amaral, M.B.P.P. Oliveira, Detection of seven genetically modified maize events in food products on the Portuguese market. Book of Proceedings of the 9º Encontro de Química dos Alimentos, 29 April to 3 May, 2009, Angra do Heroísmo, Açores, Portugal. 6. J. Costa, I. Mafra, J.S. Amaral, M.B.P.P. Oliveira, Transgenes monitoring in an industrial soybean oil processing by conventional and real-time polymerase chain reaction. Book of Proceedings of the 9º Encontro de Química dos Alimentos, 29 April to 3 May, 2009, Angra do Heroísmo, Açores, Portugal. 7. A. Ribeiro, D. Vieira, I.P. Fernandes, J.S. Amaral, F. Barreiro, -Tocopherol microencapsulation using chitosan and alginate: swelling behaviour under different pH, 11º Encontro de Química dos Alimentos, 16-19 September, 2012, Bragança, CD-ROM de atas (ISBN 978-972-745-141-8), 2012. 8. I.P. Fernandes, E. Leite, D. Vilas-Boas, J.S. Amaral, F. Barreiro, Production of chitosan based films enriched with oregano essential oil for increased antibacterial activity, 11º Encontro de Química dos Alimentos, 16-19 September, 2012, Bragança, CD-ROM de atas (ISBN 978-972745-141-8), 2012. 9. R.O. Rodriguesa, E. Pérez-Lorenzo, S. Arana, J.S. Amaral, P.S. Rodrigues, M. Mujika, Development and optimization of a biological protocol for DNA detection of Escherichia coli O157:H7 by Quartz Crystal Microbalance (QCM), 11º Encontro de Química dos Alimentos, 1619 September, 2012, Bragança, CD-ROM de atas (ISBN 978-972-745-141-8), 2012. 10. C.G.Santos, V.S.Melo, J.S.Amaral, M.B.P.P. Oliveira, I. Mafra, Meat species identification by polymerase chain reaction technique to authenticate Alheiras de caça, International Congress of Traditional Products, 3-5 May, Ponte de Lima, Portugal, 2012. 9 5.1.5. Communications in International Scientific Meetings 1. Valentão, P., Areias, F., Amaral, J., Andrade, P. Characterization of seven medicinal species by the phenolic constituents of their hydrolyzed extracts. 1st International Meeting of Aromatic th and Medicinal Mediterranean Plants, 24-26 of April, 1998, Conimbriga-Ansião, Portugal. 2. Amaral, J. Ferreres, F.; Santos, A.; Pinheiro, C.; Andrade, P.; Seabra, R. Analysis of phenolic compounds from hazelnut (Corylus avellana L.) leaves. 1st International Conference on Polyphenols and Health, 18-21th of November, 2003, Vichy, France. 3. J.S. Amaral, Citóva, I.; Casal, S. ; Santos, A.; Seabra, R.; Oliveira, B.P.P. Nutritional value and fatty acid composition of Hazelnut varieties harvested in Portugal. 6th International Congress on Hazelnut, 14-18th June, 2004,Tarragona, Spain. 4. J.S. Amaral, Matos, L.C.; Citóva, I.; Pereira, J.A.; Seabra, R.; Oliveira, M.B.P.P. Influence of the cultivar and degree of ripeness in the composition of three portugues olive oils. 5th International Symposium on Olive Growing, 27th September to 2nd of October, 2004, Izmir, Turkey. 5. J.S. Amaral, Citóva, I.; Mendes, E.; Assunção, A.; Seabra, R.; Oliveira, M.B.P.P. Sterol composition of hazelnuts from different Portuguese cultivars. Euronalysis XIII Conference, 510th of September, 2004, Salamanca, Spain. 6. J.S. Amaral,Seabra, R.M.; Oliveira, M.B.P.P. Tocopherols and tocotrienols composition of hazelnuts. EFFoST 2005 Conference, 25-28th October, 2005, Valencia, Spain. 7. Cunha, S.C.; J.S. Amaral,Fernandes, J.O.; Oliveira, M.B.P.P. Simultaneous determination of tocopherols and tocotrienols in virgin olive oils by Normal Phase HPLC coupled with DiodeArray/Fluorescence detectors. EFFoST 2005 Conference, 25-28th October, 2005, Valencia, Spain. 8. J.S. Amaral,Casal, S.; Seabra, R.M.; Oliveira, M.B.P.P. Composition of walnuts lipid fraction: A 3-year study of different cultivars. 231st ACS National Meeting, 26-30th March, 2006, Atlanta, USA. (oral communication) 9. Mafra I.; Amaral J.S.; Silva, C.F.; Oliveira, M.B.P.P. Detection of genetically modified organisms in raw and highly processed soybean and maize foodstuffs by Polymerase Chain Reaction. 3rd International Symposium on Recent Advances in Food Analysis, November 7 – 9, 2007, Prague, Czech Republic. 10. Costa, J.; J.S. Amaral,Casal, S.; Mafra, I.; Oliveira, M.B.P.P. Soybean oil from GM seeds: chemical characterization along the fat extraction and refining processes. II Encontro Nacional de Bromatologia, Hidrologia e Toxicologia and AOAC Europe Section International Workshop: “Enforcement of European Legislation on Food and Water: Analytical and Toxicological Aspects”, 17th and 18th April 2008, Lisbon, Portugal. 11. J.S. Amaral,Mafra, I.; Oliveira, M.B.P.P.; Soares, S Fatty acid profile of commercial Frankfurt type sausages. COLACRO XII - Congresso Latino-Americano de Cromatografia e Técnicas Relacionadas, 28-30 October 2008, Florianópolis, Brasil. 12. Costa, J.; J.S. Amaral,Casal, S.; Mafra, I.; Oliveira, M.B.P.P. Effect of refining on the fatty acid, sterol and tocopherols compositions of soybean oil from GM seeds. COLACRO XII - 10 Congresso Latino-Americano de Cromatografia e Técnicas Relacionadas, 28-30 October 2008, Florianópolis, Brasil. 13. Ramalho, H.M.M; Casal, S.; J.S. Amaral,Oliveira, M.B.P.P. Determinação do teor em colesterol no leite de raça autóctone portuguesa por HPLC. COLACRO XII - Congresso LatinoAmericano de Cromatografia e Técnicas Relacionadas, 28-30 October 2008, Florianópolis, Brasil. 14. Pinho, R.S.; Mendes, E.; Casal, S.; Silva, S.I.; Oliveira, Amaral, J.S; Oliveira, M.B.P.P. Identificação e quantificação de tocoferóis por HPLC em Croton adamantinus (Euphorbiaceae). COLACRO XII - Congresso Latino-Americano de Cromatografia e Técnicas Relacionadas, 2830 October 2008, Florianópolis, Brasil. 15. Soares, C.; Alves, R.C.; J.S. Amaral,Casal, S.; Oliveira, M.B.P.P.; Fernandes, J.O. Method validation for analysis of acrylamide in coffee by GC/MS – comparison between matrix solidphase dispersion and liquid-liquid procedures for sample preparation. COLACRO XII Congresso Latino-Americano de Cromatografia e Técnicas Relacionadas, 28-30 October 2008, Florianópolis, Brasil. 16. Soares, S; J.S. Amaral,Mafra, I.; Oliveira, M.B.P.P. Polymerase chain reaction for soybean detection in heat processed meat products. First European Food Congress, 4-9 November 2008, Ljubljana, Slovenia. 17. Costa, J.; Mafra, I.; J.S. Amaral,Oliveira, M.B.P.P. Comparison of different extraction methods of soybean DNA from refined vegetable oils. First European Food Congress, 4-9 November 2008, Ljubljana, Slovenia. 18. Barros, M.C., Fernandes, I.P., Amaral, J.S., Barreiro, M.F., Pinto, V., Ferreira, M.J. Chitosan as an antimicrobial agent for footwear leather components. 2nd International Conference on Biodegradable Polymers and Sustainable Composites (BIOPOL-2009), 30 September - 2 October, 2009, Alicante, Spain (oral communication) 19. Mafra, I.; J.S. Amaral,Soares, S.; Oliveira, M.B.P.P. Quantitative detection of pork’s meat adulteration in processed poultry’s meat products by Real Time PCR. Final TRACE Conference: “How to trace the origin of food?” 2nd and 3rd December 2009, Brussels, Belgium. 20. J.S. Amaral,Mafra, I.; Soares, S.; Oliveira, M.B.P.P. Detection of soybean in meat products by Taq-Man real-time PCR assay. Final TRACE Conference: “How to trace the origin of food?” 2nd and 3rd December 2009, Brussels, Belgium. 21. Costa, J.; J.S. Amaral,Mafra, I.; Oliveira, M.B.P.P. Antioxidant profiles of soybean oil obtained from genetically modified seeds. International Symposium on the Pathophysiology of Reactive Oxygen and Nitrogen Species, 19-21 May 2010, Salamanca, Spain. 22. Igrejas, G.; J.S. Amaral,Rodrigues, P.J.; M.B.P.P., Oliveira. A T-S fuzzy model based on initial FCM partition to estimate the composition of commercial blended vegetable oils. FOODSIM’2010, 24-26th June 2010, Bragança, Portugal. 23. Soares, S; J.S. Amaral, Mafra, I.; Oliveira, M.B.P.P. The use of model samples to estimate the level of fraudulent addition of pork meat in poultry meat by real-time PCR. FOODSIM’2010, 2426th June 2010, Bragança, Portugal. (oral communication) 11 24. Rodrigues, P.J.; J.S. Amaral,Santos, A.; Igrejas, G. A segmentation approach for the identification of hazelnut cultivars. FOODSIM’2010, 24-26th June 2010, Bragança, Portugal. (oral communication) 25. Mafra, I.; J.S. Amaral,Costa, J.; Oliveira, M.B.P.P. A survey of genetically modified maize in the Portuguese market. 2010 EFFoST Annual Meeting, 10-12 November 2010, Dublin, Ireland. 26. Mafra, I.; J.S. Amaral,Soares, S.; Oliveira, M.B.P.P. Quantitative detection of soybean addition to processed meat products using a real-time PCR technique. 2010 EFFoST Annual Meeting, 10-12 November 2010, Dublin, Ireland. 27. Sepúlveda, M., Fernandes, I.P., Amaral, J.S., Barreiro, M.F. Fungal degradation of lignin-based rigid polyurethane foams. 3 RD International Conference on biodegradable and Biobased polymers- BIOPOL 2011, 29-31 August 2011, Strasbourg, France. 28. Fernandes, I.P., Barreiro, M.F., Pinto, V., Ferreira, M.J., Amaral, J.S. Chitosan-based RD antimicrobial leather coatings: evaluation of the pilot scale production. 3 International Conference on biodegradable and Biobased polymers- BIOPOL 2011, 29-31 August 2011, Strasbourg, France. 29. Fernandes, I.P., Amaral, J.S., Barreiro, M.F., Pinto, V., Ferreira, M.J. Chitosan microparticles loaded with essential oils having in view leather applications. Book of Abstracts of the 11th International Chemical and Biological Engineering Conference (CHEMPOR 2011), 5-7 September 2011, Lisbon, Portugal. (oral communication). 30. Vilas-Boas, D., Leite, E., Fernandes, I.P., Amaral, J.S., Barreiro, M.F. Production of chitosan based films enriched with essential oils for biomedical applications. Japan-Portugal nano-BME Symposium 2011, 3-6 June 2011, Bragança, Portugal. 31. Santos, C.G., Melo, V.S., Mafra, I., Amaral, J.S., Estevinho, L., Oliveira, M.B.P.P. Authentication of traditional game meat products by the use of species-specific PCR, 3 MoniQa International Conference, 27-29 September 2011, Varna, Bulgaria. rd (oral communication) 32. Igrejas, G., Amaral, J.S., Rodrigues, P.S. Fall Detection System For Elderly People: A Neural Network Approach, 5th International Conference on Biomedical Electronics and Devices BIODEVICES 2012, 1-4 February, Vilamoura, Portugal (2012). 33. J. Costa, V.S. Melo, C.G. Santos, I. Mafra, J.S. Amaral, L. Estevinho, M.B.P.P. Oliveira, Comparison of DNA extraction methods to detect trace amounts of tree nut allergens in chocolates, 5th International Symposium on Recent Advances in Food Analysis, Praga, livro de resumos p 270 (e-presentation), 2011. 34. Santos, C.G., Melo, V.S., Amaral, J.S., Oliveira, M.B.P.P., Mafra, I. Meat species identification by polymerase chain reaction technique to authenticate Alheiras de caça, Book of Proceedings of the International Congress on Promotion of Traditional Food Products, 3-5 May, Ponte de Lima, Portugal (2012). 35. Amaral, J.S., Fernandes, I.P., Pinto, V., Ferreira, M.J. Barreiro, M.F., Chitosan-based leather th functional coatings with improved antimicrobial properties, 4 EuCheMS Chemistry Congress, 26-30 August 2012, Prague, Czech Republic. (oral communication) 36. Amaral, J.S., Ribeiro, A., Alves, S., Gonçalves, A., Poeta, P. Chemical composition of different oregano (Origanum vulgare L.) essential oils: relevance for the activity against foodborne and 12 spoilage bacteria, 4 th EuCheMS Chemistry Congress, 26-30 August 2012, Prague, Czech Republic. 37. Soares, S; J.S. Amaral, Mafra, I.; Oliveira, M.B.P.P. Quantitativee detection of pork’s meat by real-time PCR for Halal verification of processed meat products, EuroFoodChem XVII, 7-10 May 2013, Istanbul, Turkey. (oral communication) 38. Santos, CG; Melo, VS, J.S. Amaral, Oliveira, M.B.P.P. Mafra, I. Detection of adulterations in tradictional Portuguese game meat products by polymerase chain reaction technique, EuroFoodChem XVII, 7-10 May 2013, Istanbul, Turkey. (oral communication) 5.1.6. Communications in National Scientific Meetings 1. P. Valentão, F. Areias, J. Amaral, P. Andrade, R. Seabra, Xantonas tetraoxigenadas de Centaurium erythraea. I Congresso das Plantas Aromáticas e Medicinais dos Países de Língua Oficial Portuguesa, 7-9th June, 1999, Ansião, Portugal. 2. J. Amaral, S. Casal, J.A. Pereira, R. Seabra, M.B.P.P. Oliveira, Avaliação nutricional e composição em ácidos gordos de 6 variedades de nozes, sementes de Juglans regia L., cultivadas em Trás-os-Montes. 6º Encontro de Química dos Alimentos, 22-25th June, 2003, Lisboa, Portugal. (Comunicação oral). 3. J.S., Amaral, S. Casal, I. Citová, R.M. Seabra, B.P.P. Oliveira, Chemical composition, fatty acid and sterol contents of nuts grown in Portugal. 3º Encontro REQUIMTE, 9-10th of January, 2004, Fátima, Portugal. 4. J.S. Amaral, S. Casal, B.P.P. Oliveira, R.M. Seabra, Determinação da Vitamina E em avelãs: optimização e validação do método analítico. 7º Encontro de Química dos Alimentos, 13-16 th of April, 2005, Viseu, Portugal. 5. J.S. Amaral, S.C. Cunha, N. Neves, B.P.P. Oliveira, R.M. Seabra, Determinação do perfil de triacilgliceróis em nozes. 7º Encontro de Química dos Alimentos, 13-16th of April, 2005, Viseu, Portugal. 6. J.S. Amaral, S Casal, M.R Alves, R.M. Seabra, B.P.P. Oliveira, Lipid fraction composition and nutritional quality of walnuts (Juglans regia L.) grown in Portugal. 4º Encontro REQUIMTE 31st of Mars to 1st of April, 2005, Fátima, Portugal. 7. J.S. Amaral, S Casal, R.M. Seabra, B.P.P. Oliveira, Benefícios potenciais do consumo de nozes. I Encontro Nacional de Bromatologia, Hidrologia e Toxicologia, 2-3 June, 2006, Sesimbra, Portugal. 8. J.S. Amaral, S Casal, R.M. Seabra, B.P.P. Oliveira, Influência da torra de avelãs na sua composição em vitamina E. 8º Encontro de Química dos Alimentos, 4-7th Mars, 2007, Beja, Portugal. 9. Mafra, I. C.S. Ferreira da Silva, J.S. Amaral, M.P.P. Oliveira, Detection of genetically modified maize in foodstuffs. Micro’07-Biotec’07-XXXIII JPG Congress, 29 November – 2 December 2007, Lisbon, Portugal. 10. J. Rocha, I. Mafra, M. Carvalho, J.A. Amaral, M.B.P.P. Oliveira, Detection of authorized genetically modified maize events: Participation in an inter-laboratorial study. IJUP-08, 20-22 February 2008, Porto, Portugal (oral communication). 13 11. S.C. Sousa, I. Mafra, C.S.F. Silva, J.A. Amaral, M.B.P.P. Oliveira, Development of molecular biology techniques for the detection of genetically modified organisms in maize food products. IJUP-08, 20-22 February 2008, Porto, Portugal. (oral communication). 12. J. Costa, I. Mafra, J. Rocha, J.S. Amaral, M.B.P.P. Oliveira, Detection of soybean DNA in commercially available vegetable oils. XXI Encontro Nacional da Sociedade Portuguesa de Química, 11–13 June, 2008, Porto, Portugal. 13. S. Soares, J.S. Amaral, I. Mafra, M.B.P.P. Oliveira, Quality control of Frankfurt sausages by biomolecular techniques. XXI Encontro Nacional da Sociedade Portuguesa de Química, 11–13 June, 2008, Porto, Portugal. 14. J.S. Amaral, R.M. Seabra, M.B.P.P. Oliveira, 1º Encontro Nacional de Química Terapêutica, 13-15 November, Porto, 2008, Portugal. 15. J.S. Amaral, S. Soares, I. Mafra, M.B.P.P. Oliveira, Polymerase chain reaction for the detection of allergens: the case of soybean in processed meat products. 9º Encontro de Química dos Alimentos, 29 April to 3 May, 2009, Angra do Heroísmo, Açores, Portugal. 16. S. Soares, J.S. Amaral, I. Mafra, M.B.P.P. Oliveira, Species identification in heat processed meat products. 9º Encontro de Química dos Alimentos, 29 April to 3 May, 2009, Angra do Heroísmo, Açores, Portugal. 17. I. Mafra, J.S. Amaral, M.B.P.P. Oliveira, Detection of seven genetically modified maize events in food products on the Portuguese market. 9º Encontro de Química dos Alimentos, 29 April to 3 May, 2009, Angra do Heroísmo, Açores, Portugal. (oral communication) 18. J. Costa, I. Mafra, J.S. Amaral, M.B.P.P. Oliveira, Transgenes monitoring in an industrial soybean oil processing by conventional and real-time polymerase chain reaction. 9º Encontro de Química dos Alimentos, 29 April to 3 May, 2009, Angra do Heroísmo, Açores, Portugal. (oral communication) 19. J.S. Amaral, S. Soares, I. Mafra, M.B.P.P. Oliveira, SYBR Green I Real-time polymerase chain reaction as a tool to detect poultry’s meat adulteration with pork’s meat. MICROBIOTEC'09, 2830 de Novembro, 2009, Vila Moura, Portugal. 20. I. Mafra, J.S. Amaral, S Soares, M.B.P.P. Oliveira, Real-time polymerase chain reaction for the quantitative detection of soybean in meat products. MICROBIOTEC'09, 28-30 de Novembro, 2009, Vila Moura, Portugal. (oral communication) 21. J. Costa, T.J.R. Fernandes, I. Mafra, J.S. Amaral, M.B.P.P. Oliveira, Soybean DNA extraction from blended refined vegetable oils. IJUP’10, 3rd Meeting of Young Researchers at UP, 17-19 February 2010, Porto, Portugal. 22. A. Ribeiro, D. Vieira, I.P. Fernandes, J.S. Amaral, F. Barreiro, “-Tocopherol microencapsulation using chitosan and alginate: swelling behaviour under different pH”, Livro de resumos do 11º Encontro de Química dos Alimentos, 16-19 September, 2012, Bragança, p. 172. 23. I.P. Fernandes, E. Leite, D. Vilas-Boas, J.S. Amaral, F. Barreiro, “Production of chitosan based films enriched with oregano essential oil for increased antibacterial activity”, Livro de resumos do 11º Encontro de Química dos Alimentos, 16-19 September, 2012, Bragança, p. 342. 14 24. R.O. Rodriguesa, E. Pérez-Lorenzo, S. Arana, J.S. Amaral, P.S. Rodrigues, M. Mujika, “Development and optimization of a biological protocol for DNA detection of Escherichia coli O157:H7 by Quartz Crystal Microbalance (QCM)”, Livro de resumos do 11º Encontro de Química dos Alimentos, 16-19 September, 2012, Bragança, p. 271. Participation in Projects 1. Member of the Bragança Polytechnic Institute team in the Project CYTED “Red V.F:Red Iberoamericana de Adsorbentes para la protección Ambiental”, 2001-2005 2. Member of the project "AdvancedShoe - Integration of advanced solutions for materials and development of footwear product of high-performance," Project No. 1585, QREN (consortium of 10 institutions, 4 of R&D, including IPB, FCUP, CTC, PIEP) 2008-2011 3. Principal researcher of the Project “Authentication of "Alheiras" based on meat species identification using molecular biology techniques”, Projectos Pluridisciplinares IJUP- código 185, funding institution: Universidade do Porto (UP) / Santander Totta, 2010-2011. 4. Principal researcher of the project “Detection of soybean addition in meat processed products by a real-time PCR approach”, Projectos Pluridisciplinares- nº. 149, funding institution: Universidade do Porto (UP) / Santander Totta, 2011-2012. 5. Member of the Project “Application of real-time PCR to detect hazelnut as a potential hidden allergen in chocolates”, Projectos Pluridisciplinares- nº. 176, funding institution: Universidade do Porto (UP) / Santander Totta, 2011-2012. 6. Member of the Project “DNA barcodes for Hypericum species identification in herbal teas”, Projectos Pluridisciplinares- nº. 290, funding institution: Universidade do Porto (UP) / Santander Totta, 2011-2012. 7. Member of the project “NEWALK - Materials, components and technology for footwear” (Projeto Mobilizador de Investigação QREN-ADI-NEWALK-13850), funding institution: QREN, 2011-2014. 8. Member of the project “BIOMIMETIC - A biomimetic microdevice for the diagnosis of erythrocytes diseases based on their deformability” (PTDC/SAU-ENB/116929/2010), funding institution: Portuguese Foundation for Science (FCT), 05-03-2012 until 04-03-2015. 9. Principal researcher of the project “Safety of Plant Food Supplements: searching for adulterant pharmaceutical drugs and plants” (EXPL/DTP-SAP/1438/2013), funding institution: Portuguese Foundation for Science (FCT), 2014 – 2015 (approved). 5. Other data Researcher member of the Associated Laboratory REQUIMTE from 2002 to present date. From May 2009-September 2012 was the President of the Food Chemistry Division of the Portuguese Society of Chemistry. From July 2011 to present date is a represent of the Portuguese Society of Chemistry in the Food Chemistry Division of EuCheMS (European Association for Chemical and Molecular Sciences). 15 Member of the Editorial Board of Food Science and Technology, Horizon Research Publishing, USA. 6. Current research interests Application of analytical techniques, mainly chromatography, in the study of nutraceutical and phytochemical compounds in foods. Application of molecular biology techniques (PCR and conventional Real-Time PCR) in food safety and authenticity studies, particularly with the detection of genetically modified organisms (GMOs) allergens (soybean, nuts) and the detection of ingredients (pig, cow, poultry, soybean) in processed foods. Antimicrobial activity of natural products. 16