2011 5 (2)
I LUSO-BRAZILIAN CONGRESS OF THE EXPERIMENTAL PATHOLOGY
XI International Symposium on Experimental Techniques
Federal University of Pernambuco
CPE-1360
1
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sponse to Cyclosporin A
Ivana Jarak, Ludgero C. Tavares, John G. Jones, Rui A.
Carvalho
Center for neuroscience and Cell Biology, Coimbra, Portugal
Cyclosporin A (CsA) is an immunosuppressive drug frequently used for prevention of allograft rejection and the
treatment of autoimmune diseases. Despite the desired
effect on the immune system, long-term use of CsA is
associated with adverse effects that have adverse impact
on the quality of life and the survival of patients. Acute
rotoxicity, and renal dysfunction. Although the underlying molecular mechanisms of CsA toxicity are not fully
understood, they involve oxidative stress, apoptosis and
rapid and multicomponent analysis of all the important
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of Pernambuco, Brazil
2. Department of Pathology - Federal University of Pernambuco,
Brazil
development of laceration in the middle layer from intima
-
secting hematoma were collected from cadavers were em-
where investigated sex, age and personal history. Results:
The mean age was 71.8 years for male when compared
with 56.5 years old in the female. Among the diseases are
more common in men, the hypertension cases involving
of the male cases, moderate atheromatous lesions were
toma was installed in the proximal segment (thoracic) at n
evaluate the hepatic response to CsA treatment and to asment of detrimental side effects of CsA treatment. Thus,
16 non-transplanted Wistar rats were treated for 2 weeks
livers freeze-clamped for analysis. The same experiments
were performed after one day CsA administration. Meta-
the aortic aneurism dissecans is noted in women with a
-
lised liver tissue samples. 1H NMR spectra were acquired
at 14.1 T Varian spectrometer equipped with a 3-mm in-
women.
Keywords: Dissecans aortic aneurism; Macroscopic;
Epidemiology
nent analysis (PCA), using a Spearman correlation matrix
CPE-1374
Nitric oxide is responsible for oxidative skin injury
after 24h of UVB exposure
V. A. Terra1, F. P. Souza-Neto1, L. N. Z. Ramalho2, R. C.
Pereira1, T.N.X. Silva1, R. Cecchini1, A. L. Cecchini1
1. Laboratório de Fisiopatologia e Radicais Livres, Universidade
Estadual de Londrina (UEL). Rodovia Celso Garcia Cid, PR445, km 380, Campus Universitário, 86051-990, Londrina, PR,
Brasil.
2. Dept. Patologia e Medicina Legal, Universidade de São Paulo
(FMRP-USP), Ribeirão Preto, São Paulo, Brasil
XLSTAT 2009 (Addinsoft). We found that although the
visual comparison of normalized spectra, which allows for
tentative population distinction under given treatment regime (2 week CsA administration). PCA analysis of lipid
extract NMR spectra indicate changes in energy (tendency
toward glycogen depletion, increased acetate and alanine
levels) and lipid (decreased levels of choline intermediary
.
CPE-1372
Macroscopic and epidemiological analysis of cases
of dissecans aortic aneurysm
1
.; Araújo Duke, M.A 1.; Modesto
Araújo,M 2.; Campos Maia, L 2.; Pontes Filho, N. T 1,2.;
Melo-Junior, M. R 1,2.
1 - Immunopathology Laboratory Keizo Asami - Federal University
mammalian cells and plays a variety of regulatory roles
jectives: This article provides evidence regarding the par-
radiation the skin was used to determine the redox capacity
9
Experimental Pathology and Health Sciences
munohistochemistry was also performed for proliferating
cell nuclear antigen (PCNA), nitrotyrosine, and vascular
endothelial growth factor (VEGF). Results: Considering
A highly sensitive NO detection system using chemilumi-
140.6) and the increase in the NO levels (41.0 x 106 ± 9.52
of NO in this organs.
Keywords: Nitric oxide, chemiluminescence, biological systems
mice were treated with aminoguanidine (AG) lipid peroxi±142.7) and shows decrease in NO levels (19.5 x 106 ±
2.7 x 106), control (15.4 x 106 ± 1.3 x 106). PCNA and
CPE-1376
Epidemiological analysis of tetanus in accidental
Brazil during the period 2008 to 2011
indicating an increase in proliferative potential when iNOS
lated to lipid and protein oxidative cell injury. Through, it
is evident that NO participation is essential to degenerative
radiated skin. This work shows that the NO play an essential part in the modulation of proliferative and oxidative
1.Departamento de Enfermagem – UFPE/ Brazil
2.Departamento de Ciências Biológicas – UFPE/ Brazil
Background: Tetanus is an acute infectious disease, not
ioral risk than as a disease, thus not presented in epidemic
Keywords Nitric oxide, lipid peroxidation, antioxidants, UVB skin irradiation, proliferation markers,
nitrotyrosine
tetanus reported in Brazil. Methodology: For this we used
CPE-1374a
Nitric oxide detection by luminol-H2O2-induced
chemiluminescence in biological system and chemical NO- donors systems
Terra, VA1; Panis, C1,2; Yamamoto, C1; Souza, FP1, Cecchni, AL1, Cecchini, R1.
1.Laboratory of Physiopathology and Free Radicals, Department
of General Pathology, State University of Londrina, Londrina,
Paraná, Brazil.
2.Stem Cells Laboratory, National Institute of Cancer, Rio de Janeiro, Brazil.
ological tension of oxygen (O2) and its high reactivity
jective: In this study our purpose was to compare and
tive of NO with the luminol – hydrogen peroxide (H2O2)
-
race, area of residence, education and evolution) that are
associated with incidence and mortality of tetanus. Results:
During this period 1137 cases were reported, of which
clusion: According to the data, the male is the most afindividuals in adulthood, unlike women that there is a vaccine strategy, aimed at prevention neonatal tetanus, during
a need to make an expansion of coverage, especially for
those individuals most affected, in addition to having an
improvement of access and assistance of health services.
Keywords: Accidental tetanus. Epidemiology. Clostridium tetani.
CPE-1376a
nambuco, from 2009 to 2011
prevented fast NO oxidative degradation through removal
analysis was in fact NO. We can conclude that this reaction
gate the nature of reactive species enrolled in NO-chemiluminescent reaction, all evaluated NO-donnors systems
1.Departamento de Enfermagem – UFPE/ Brazil
2.Departamento de Ciências Biológicas – UFPE/ Brazil
Background: Schistosomiasis is a parasitic infection caused
Schistosoma mansoni, to which the symptoms will depend
on the evolution of the parasite in the host, and man its
main reservoir. The transmission of the human through
infected snails. Infection may present as acute and the
clinical picture progressively evolves to chronic infection
reaction and on the same concentrations. Results: The re10
2011 5 (2)
tion. In a period of three to seven weeks of exposure, the
picture can emerge of acute schistosomiasis, also known
This study aimed to analyze the frequency of individuals
It was made the use of Sistema Nacional de Agravos de
area of residence and course of infection, which are associated with incidence and mortality of schistosomiasis.
Results: During this period, 1938 cases were reported, of
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for more effective programming strategies in relation to
the control of transmission, as well as the performance of
of individuals affected, with a focus on higher risk group.
Keywords: Schistosomiasis, Public health, Epidemiology
CPE-1379
Effect of essential oil of Mentha crispa on young
worms of Schistosoma mansoni and histomorphometric
analysis of liver tissue in experimental schistosomiasis
Oliveira, A.P.1; Franco, E.S.1; Melo, R.G.1; Aires, A.L.2;
querque, M.C.P.2; Maia, M.B.S1.
1. Department of Physiology and Pharmacology UFPE, Brazil
2. Laboratory of Immunopathology Keizo Asami - LIKA/
UFPE, Brazil
3. Faculty of Pharmacy, University of Porto, Portugal
4. Department of Pathology UFPE, Brazil
5. Department of Antibiotics UFPE, Brazil
Background: The schistosomiasis mansoni is a grave
tion is the main mechanism involved in the pathogenesis.
Studied show resistance to treatment with praziquantel, in
The aim of this study was to evaluate the antischistosomal
activity of essential oil of Mentha crispa (EOMc) on S.
mansoni infected mice. Methodology: Forty female Swiss
mice, weighing around 20-25g, infected with 50 cercariae
tion in the diameter of the granulomas (349.29±7.96,
320.72±10.24 and 355.89±11.38µm) in the groups treated
compared to control 393.86±6.81µm. Conclusions: Treatment with EOMc demonstrated schistosomicidal properties on young worms of S. mansoni, improvement of
hepatoesplenomegaly and reduction of the diameter of
the granulomas.
Keywords: Schistosoma mansoni, Mentha crispa, essential oil
CPE-1379a
Evaluation of the expectorant activity of acid fumarprotocetrárico isolated from the lichen Cladonia verticillaris in swiss mice
Alves, G.M.B.1; Franco, E.S.2; Oliveira, A.P.2; Cunha, H.
P.2; Melo, R.G.2; Cordeiro, D.P.2; Pereira, E.C.G.3; Silva,
N.H.1; Maia, M.B.S.2
1 Department of Biochemistry and Physiology – CCB/UFPE,
Brazil
2 Department of Physiology and Pharmacology – CCS/UFPE,
Brazil
3 Department of Geographical Sciences – CFCH/UFPE, Brazil
cine since ancient times to treat many kinds of respira-
verticillaris in swiss mice. Methodology: We used 30 swiss
-
after treatment the animals where anesthetized, tracheostomized and cannulated, and we carried out a cycle of
ing a spectrophotometer at 535nm. Results: The results
expressed as mean ± standard error of mean. And these
infection the animals were treated oral with 60, 80, 100
ten consecutive days. In the 47th day after infection the
mice were euthanized for evaluate of the worm load, oo-
± 0.012 and 0.285 ± 0.003, respectively) than that shown
-
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expectorant activity in mice.
Keywords: acid fumarprotocetraric, expectorant, lichens
served in liver weight (7.22±0.28, 7.11±0.53 e 7.54±0.52)
CPE-1380
Analysis of clinical requirements associated with
drug interactions of a public hospital in Pernambuco
11
Experimental Pathology and Health Sciences
1.ASCES University, Caruaru-PE
2.Microbiology Laboratory-UFPE-PE
Background: The simultaneous use of drugs, common
age, occupation, location of the lesion and assessing the
We conducted a descriptive analysis of the data and the
modify the activity and pharmacological response. In patients taking ten to twenty drugs for interactions incidence
the location of the lesion to the inner surface of the lower
includes the ongoing evaluation of the relationship for
tion of the hypotheses suggested in the clinical diagnosis
which the results outweigh the risks to which patients are
interaction with major related to drugs-patients and the administration. OBJECTIVES: Analyze the clinical requirements of the hospital identifying incidence of drug interactions. METHODOLOGY: A total of sixty prescriptions
prescriptions contained ten or more medications that were
analyzed individually considering the potential for drug-
at least two interactions occurring up to seventeen interactions in the same prescription. The drugs clonazepam,
quent prescriptions in the hospital environment, especially
where there is no clinical pharmacy service. These drug incus on therapies involving ten or more drugs which often
tween the most prevalent lesions with lesion location and
tention of health services for patients with this oral condition.
Keywords: Biopsy, Mouth Sores
CPE-1381
Salivary glands in complementary treatment experimental hyperthyroidism
Alves da Silva, M.A.1,2, Nery da Silva, E.1,2, Araújo Félix
Junior, J.1,2, Campos Oliveira, M.1,3, Souza Freitas,V.1,3,
2
Farinha Henrique Marques, R.H.2
1. Health Department - State University of Feira de Santana,
Brazil
2. Experimental Pathology Service, Faculty of Medicine, University
of Coimbra, Portugal
3. Oral Cancer Center - State University of Feira de Santana,
Brazil
-
the effectiveness of treatment and may cause damage to
patient health.
Keywords: Drug Interactions, Drugs.
of levothyroxine for two weeks. The aim of this study is
CPE-1380a
Analysis of the prevalence of oral clinical lesions in
two cities of Pernambuco
Bianco, L.R.¹, Neves, M.L.R.¹ ,Fiho, L.M.S.¹
1.ASCES University, Caruaru-PE
in four groups: control group, kept with no manipulation
(Ctrl); group II, which received an administration of 250
prevalence of oral lesions demonstrates the great diver-
even the geographic characteristics of each local. Knowing prevalent injuries and demographic characteristics of
the population affected is important for prevention and
malignant potential reinforces the importance of this ex-
lesions performed in referral centers in two cities of Per12
of acini parotid gland area on experimental hyperthyroid-
group III received levothyroxine and acupuncture treatture treatment. The points used for puncture were: ST09,
ST36, HC4, RM23. After two weeks, all the animals were
collected and analyzed using ImageJ®. The histopathological analysis shown no signs of glandular lesion. The
more cases of smaller parotid gland acini on the acupuncture treated group. The group with experimentally induced
hyperthiroidism, as the group with hyperthiroidism plus
acupuncture treatment, presented more acini with major areas than the group kept with no manipulation. The
results of the present study, although without statistical
thiroidism, individually, affects the parotid salivary gland.
2011 5 (2)
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when compared with the control group. Molecular and
analysis results.
Keywords: Salivary Glands; Morphometrics, Hyperthyroidism
CPE-1383
Determination of changes in production of polysaccharide capsule in pathogenic isolates of Klebsiella pneumoniae
Gonçalves, G. G. A.1; Veras, D. L.1, 2; Lopes, A. C. S.3;
Feitosa, A. P. S.1, 2; Freitas, C. F.1; Vaz, G. S.1; Padilha, R.2;
Brayner, F. A.1, 2, 3; Alves, L. C.1, 2.
1. Departamento de Parasitologia do Centro de Pesquisas Aggeu
Magalhães CPqAM / FIOCRUZ, Brazil
2. Laboratório de Imunopatologia Keizo Asami – LIKA/UFPE,
Brazil
3.Departamento de Medicina Tropical da Universidade Federal de
Pernambuco-UFPE, Brazil
and pneumonia. Most of these infections are associated
with hospital environments, where it is still one of the
polysaccharide capsule (CPS) consists in one of the most
important virulence factors of pathogenics isolates of K.
pneumoniae, the aim of this study was to determine the
presence of genes for the production of CPS in isolates of
K. pneumoniae, as well as to determine the time-kill and
ilar concentrations to those levels reached in the human
plasma. Methodology: During this study a total of four
and aztreonam, respectively. The Transmission Electron
es in the CPS production, after the treatment of two isolates with cefotaxime, ceftazidime and aztreonam in the
Results: All isolates presented the gene for CPS production
showed morphological changes and electrondense parti-
the symptoms more commons are fever, shivers, nightly
sweat, loss of appetite and weight, caugh (over 3 weeks).
odology: It was used the Sistema Nacional de Agravos de
race, age group, schooling, form and situation of close.
Result: There was predominance of the cases among peoto (51,87) with age group among 20 to 39 years old (45,98)
gave up the treatment. The most incidence of the cases
opment proportion to the cure regarding the desertion of
the treatment is an indicative that the health actions have
gies they refer to the promotion, control and the acting of
the epidemiologic vigilance mains to promote the prevention, cure and reduction of the transmission risc.
Keywords: tuberculosis, epidemiologic vigilance, epiCPE-1385
Effect of swimming in peripheral nerve regeneration
after experimental neurotmesis
Moraes, S.R.A.1;
1. Departament of Anatomy, Universidade Federal de Pernambuco,
Brazil.
2. Faculty of Medicine, Universidade Federal do Ceará, Brazil.
3. Departamento f Anatomy, ICB III, Universidade de São Paulo,
Brazil.
Background: The injuries of the peripheral nerves have
negative impact on the functionality of the individual,
can help in the process of peripheral nerve regeneration.
exercise in water on the peripheral nerve regeneration after injury neurotmesis. Methodology: Were used 14 male
rats (Wistar), randomly separated in the Control Group
the CPS production. Conclusions: The results showed that
days of life, the GLN group was adapted to the aquatic
in reducing the CPS production.
Keywords: Klebsiella pneumoniae; Polysaccharide
neurotmesis sciatic nerve surgery of the right foot, and the
with Matrigel® and saline solution. 48 hours after the injury, the GLN group started swimming protocol lasting 30
minutes, 5 times a week, for 28 days, while the GLSN was
CPE-1384a
during the period 2008 to 2010
Santos, C.R.C¹ ; França, M.J.D.M¹; Soares, L.H.S¹; Da
Silva, P.K.F¹; Avelino, J.E¹; Leal, T.V¹; Lima, V.P1; Júnior,
60 days of injury, all animals had their right sciatic nerve
collected for histomorphometric analysis. The statistical
1
1 Universidade Federal de Pernambuco, Brasil
13
Experimental Pathology and Health Sciences
injury was lower than in the control group, no having difference when compared GLSN and GLN groups. There
Ethics Committee of the Health Sciences Center of the
GLN, compared to the GC and the GLSN. Conclusion:
The swimming protocol adopted optimized the process
complete lesion of the sciatic nerve, without compromising the regenerative process.
Key-words: Neurotmesis; Nerve Regeneration;
Swimming
CPE-1387
Advances in the diagnosis of cervical cancer caused
by HPV
Nunes, M. K. S.1,2; Gomes, A. M. P.1,2; Silva, B. D. F.1;
Machado, W. G. S.1; Santos, E. P.1; Souza, J. P1, 2; Santos,
A. H.1; Cardoso, H.M1; Pereira, B. D. S.1; Costa, L. A.1;
1. Faculdade Santa Emília de Rodat, FASER, Graduandos em
Biomedicina, João Pessoa, PB, Brasil.
2. Universidade Federal da Paraíba, João Pessoa, PB, Brasil.
lem. However, improvements in diagnosis and prognosis
discovery that human papillomavirus (HPV) infection is
a crucial part of the causal pathway of the pathogenesis
screening processes and has asking for more investigawith the screening improvement, advances were validated
to increase the detection of early stages of neoplastic diseases. Early detection allows not only the surgical treat-
advances were achieved in three main areas: protein prowith mass spectrometry techniques and cutting-edge highperformance validation. These new technologies represent
markers in order to incorporate in the future these applications in molecular diagnosis of cervical cancer.
Keywords: Advances, diagnosis, cervical cancer
clusion: The main heart diseases were: atrial septal defect
cords. It is recommended to health professionals to keep
Keywords: Neonatal Nursing, Congenital Heart Disease, Newborn
CPE-1391
Investigation of cytotoxicity of plant extracts in
northeastern Brazil
Vilar, G.N.1; Melo, A.F.C.1; Trindade, A.S.P.1, Brito,
I.M.S.C.1; Costa, V.C.O.1; Silva, M.S.1; Tavares, J.F.1; Silva,
T.M.S.2; Cavalcanti, F.A.3; Pessôa, H.L.F.1
1.Departamento de Biologia Molecular, Universidade Federal da
Paraíba, Brazil
2.Departamento de Química, Universidade Federal Rural de Pernambuco, Brazil
3.Instituto de Ciências Biológicas e da Saúde, Universidade Federal
da Alagoas, Brazil
Solanum paniculatum L. and Croton grewioides Baill. are
used in folk medicine for treatment of gastrointestinal diseases. The evaluation of potencial toxicity of natural prod-
a simple model o study protective and toxic effects of a
vestigate the cytotoxicity of ethanolic extracts of the stem
oides through hemolytic and hemagglutinating evaluation.
Methodology: Blood sample (A, B and O) was mixed with
lution of NaCl. The extracts were added to suspension of
erythrocytes at various concentrations (1, 10, 100 and 1000
perature under 100 rpm. After this time they were centrifuged at 2500 rpm for 5 minutes and hemolysis was mea-
CPE-1390
Heart Diseases in the neonatal period in a hospital
school of Recife - PE
Silva, V. B.¹; Ferraz, I. C.¹; Silveira, M. M. B. M.¹; Siqueira,
D. C. C.1; Silva, D. M.1;
1. Dep. de Enfermagem - Universidade Federal de Pernambuco
(CAV) – Brasil
the time the child reaches the age of 27 days, 23 hours
to induce or prevent de clot formation of erythrocytes of
investigate the congenital heart diseases in the neonatal
period in a hospital school in the city of Recife, in the
period January to June 2011. Methodology: A descriptive
study was conducted exploratory in nature cross through
cytotoxicity e no effect on hemagglutination.
Keywords: Plant extracts, hemolysis, hemagglutination
period January to June 2011. For collecting data was drawn
14
CPE-1393
Seroprevalence of HIV and syphilis among inmates
from Caruaru, Pernambuco, Brazil
-
2011 5 (2)
cena, W.A.T.1; Lima, P.C.S.1; Silva, L.S.F.2; Guedes, R.B.2,
Brito Tiago, G.G.3
1 Faculdade Associação Caruaruense de Ensino Superior
(ASCES), Caruaru, Brazil
2 Setor de Enfermagem do Presídio Juiz Plácido de Souza, Caruaru,
Brazil
3 Laboratório do Hospital Casa de Saúde Bom Jesus, Caruaru,
Brazil
Background: The prison population is a high risk group
for HIV and Syphilis, so screening test and counseling
and to identify risk factors for these infections among
We conducted a cross-section in the period from May
to July 2011, where 1,097 inmates at a prison in Caruaru were evaluated. We performed a structured interview
respectively, have already come into contact with these
protective equipment. However two participants reported
not using all this equipment. Conclusion: Although students have not found seropositive for HCV, many assessed
through needlestick accidents with sharp material thus
meriting a further analysis in this population.
Keywords: Hepatitis C, dentist, prevalence
CPE-1394
Screening for antimicrobial activity among bacteria
isolated from amphibians (Anura)
Duarte, S.S.; Monteiro, S.C.G.; Gadelha, C.A.A.; SantiGadelha, T.; Pessôa, H.L.F.
Departamento de Biologia Molecular, Universidade Federal da
Paraíba, Brazil
Background: A powerful approach to discover novel me-
(MHA-TP). Results: The prevalence of HIV infection was
10.1 years, with a minimum age of 18 and maximum of 94
-
The association with HIV infection was statistically sig-
transmitted infection. Conclusion: The prison population
is a group at high risk for these diseases and screening tests
ics. Methodology: Bacteria were grown in Mueller Hinton
ture media were centrifuged at 6000 rpm for 15 min and
the supernatants were frozen for 24h. The resulting lysates
7 isolates was carried out against 7 indicator strains: Bacilerichia coli ATCC 10536, Pseudomonas aeruginosa ATCC
9027, Pseudomonas aeruginosa ATCC 25619, Staphylococcus aureus ATCC 6538, Staphylococcus aureus ATCC
growth was evaluated. The resistance of the isolates to
amoxicillin, ampicillin, gentamicin, streptomycin and tet-
need to implement prevention programs so that they will
not spread within this population.
Keywords: HIV, Syphilis, Prison
CPE-1393a
Seroprevalence and risk factors for hepatitis C virus in
students of dentistry in Caruaru-Pe
K.B.1, Menezes, A.F.1
1.Faculdade Associação Caruaruense de Ensino Superior –
ASCES
Background: The working conditions of dentists do with
All the isolates were active against E. coli ATCC 2536, P.
aeruginosa ATCC 25619 and S. aureus ATCC 25925. One
and P. aeruginosa ATCC 9027. Two isolates were resistant
zones higher than 11 mm. All isolates were resistant to
ampicillin and only two were resistant to gentamicin and
streptomycin. Conclusion: The results indicate that the
potential of these microorganisms to produce antimicrodrugs.
Keywords: Amphibians, antimicrobial, bacteria
risk factors for HCV in students of dentistry in CaruaruPE. Methodology: Was conducted a cross-section in the
in the last two periods of the dentistry course were evaluated. Was performed a structured interview and some data
were no students tested positive for anti-HCV. Character-
CPE-1399a
Acute toxicity of essential oil from leaves of Rollinia
leptopetala RE Fries in mice
Moreira, M.M.B.1; Brito, M.T. 1; Mangueira, V.M. 1; Batista, T.M1; Viana, W.P.1; Pessoa, D.R1; Sousa, T.K.G.1;
Tavares, J.F.1; Diniz, M.F.F.M.1; Castello Branco, M.V.S.1
1. Laboratório de Tecnologia Farmacêutica, Universidade Federal
da Paraíba, Brazil
respecting their toxicological risks. Rollinia leptopetala RE
15
Experimental Pathology and Health Sciences
Keywords: Preterm, socioeconomic, maturity
ate the safety of essential oil from leaves of Rollinia leptopetala (E.O.R) determining its potential toxicity. Methodology: For the acute study, the E.O.R. was administered
determined for up to 14 days. Indexes of organs (liver,
heart, kidneys, spleen and thymus) were evaluated to de-
effects, such as hypnosis, ptosis, sedation, constipation,
after the i.p. administration and were more pronounced
-
CPE-1401
Evaluation of antimicrobial activity and genotoxicity
of plant extracts in northeastern Brazil
Melo, A.F.C.1; Vilar, G.N.1; Brito, I.M.S.C.1; Saraiva,
M.G.1, Costa, V.C.O.1; Silva, M.S.1; Tavares, J.F.1; Silva,
T.M.S.2; Cavalcanti, F.A.3; Pessôa, H.L.F.1
1. Departamento de Biologia Molecular, Universidade Federal da
Paraíba, Brazil
2. Departamento de Química, Universidade Federal Rural de Pernanbuco, Brazil
3. Instituto de Ciências Biológicas e da Saúde, Universidade Federal
da Alagoas, Brazil
Introduction: Plants supply most of the active ingredients
well documented that gene mutations act in the process
of carcinogenesis and assays that detect genotoxic comTul., Solanum paniculatum L. and Croton grewioides Baill.
perimental animals. Conclusions: E.O.R. showed moderate
acute toxicity when administered i.p. to mice. RepeatedKeywords: Rollinia leptopetala; acute toxicity; essential oil
CPE-1400
Preterm birth and the possible implications of the socioeconomic background and the maternal maturity
Pereira, A.F.¹; Alves, I.S.¹, Menezes, R.C.L.¹; Anjos, F.B.R¹,
tory Concentration (MIC). It was used Gram-negative and
erichia coli ATCC 2536 and ATCC 10536, Pseudomonas
aeruginosa ATCC 8027 and ATCC 25619, Staphylococcus
(630 nm). The genotoxicity of three species extracts (1, 10,
100 and 1000 ug) was evaluated in Salmonella thiphymurium strains TA 97, TA 98, TA 100 and TA 102 through
1-Universidade Federal de Pernambuco – Brazil
2-Maternidade Professor Bandeira Filho – Brazil
and socioeconomic level of the pregnant women attended
in the Professor Bandeira Filho maternity (PBFm). Methodology: This research was transversal, trough semi-direct
questionnaires applied in PBFm, in the years of 2006 and
2007. The analyzed data were: family income, education
strains tested and showed no genotoxic effect. Conclusion:
toxic potencial.
Keywords: Plant extracts, antimicrobial activity,
genotoxicity
CPE-1386
Research of helminths larvae in the main beaches
from João Pessoa, Brazil
De Sousa, T.A.1; Almeida, R.N.1; Leite, G.G.F1; Gomes,
A.M.P.1 ; Santos, E.P.1; Cardoso, H.M.1;Santos, S.G.2
1. Santa Emília de Rodat College, FASER, Students of Biomedicine, João Pessoa, PB, Brazil.
2. Santa Emília de Rodat, College,FAZER. Sócrates Golzio Biomedicine professor of the clinical biochemistry and parasitology subject. João Pessoa,PB, Brazil.
main cause of contamination the faeces of domestic ani-
disciplinary team may offer an alternative to correct these
elements also seen in this population.
16
cara sp. and the Cutaneous migrans larvae, which are the
main etiologic agents Ancylostoma sp. and the Strongiloi-
2011 5 (2)
performed mainly in the aspect to diagnose precisely and
consequently to treat the association of pathologies.
Keywords: Advances, diagnosis, Kaposi sarcoma
so that four have formed a square with 10 m each side,
and one in the center of the square, the depth in which
-
CPE-1389
Liver and adipose tissue in experimental DMBA administration
Patricia Jesus1, Pedro Sá2, Nuno P. F. Gonçalves1
2
Canelas1, Mariette M. Pereira1
1. Chemistry Department, University of Coimbra
2. Institute of Experimental Pathology, Faculty of Medicine - University of Coimbra
-
de Gramame; Praia do Sol, and Seixas. From the larvae
cluded that it is necessary preventive measures to avoid
some tissues and develop a carcinogenic action. The goal
of this study is to quantify the presence of DMBA in liver
and adipose tissue in the experimental short-term intoxica-
sand contamination.
Keywords: Helminths; larvae; beaches
CPE-1386a
Kaposi sarcoma (SK) associated to the acquired im-
weeks; and the control group, kept on the standard conditions. At the end of the assay time, all animals were ethically killed and the complete necropsy performed. Frag-
Cardoso, H.M.1; Sousa, T.A.1; Santos, A.H.1; Silva, S.R.1;
Gomes, A.M.P.1,2; Nunes, M.K.S 1,2; Silva, B.D.F.1;
for routine histopathological processing and examination,
and for snap freezing in liquid nitrogen and preservation
at -80ºC. A liquid-liquid extraction protocol was used to
isolate the lipophilic portion of the liver and adipose tissue
de Carvalho, M.M.S.3.
1. Santa Emilia de Rodat Faculty, student of Biomedicine, Brazil
2. Federal University of Paraiba, student of Technical Biodiagnostic, Brazil.
3. Biological Science Center, State University of Paraiba, Brazil
Introduction: The SK is a proliferative neoplasm associated to immunosuppression conditions that affects the
The Lee index was calculated and showed only one animal
with values under 0,30. This animal had the higher scores
sue) and the higher scores concerning to edema on the
human herpes virus type 8 (HHV8). In patients with the
cy in male individuals. In a clinical point of view, the SK
identify how occurs the association of SK with AIDS, in
of known literature, as well as a critical and comparative
Results and Discussion: In the systematic research from
innovations in the diagnosis, as one of the studies that suggests the roles of the cocktail ABCB1 and other drugs in
phasized the aspect treatment where one of the studies has
evaluated the gene segmentation associated to varied doses
of gaciclovi and zidovudina getting results that the target
animals presented adipose tissue haemorrhage and conlesions, particularly of the liver, and the measured quantity of DMBA. The DMBA concentrations found on liver
are related with the doses found on adipose tissue, with
greater concentrations on the last one. The results of this
the adipose tissue, with a correlation with the concentration on the liver.
CPE-1395
Evaluation of awareness: Breast cancer and cervical
cancer with teachers from private schools in the city
of Patos-PB
Fernanda D.¹, Pereira, Joana D. C.¹, Dutra, Maria M. G.¹,
AIDS mainly in the regions from the African continent
1. Graduating in Biomedicine- Faculdades Integradas de Patos –
FIP.
2. Lecturer in Biomedicine- Faculdades Integradas de Patos – FIP.
present great incidence of the SK related to HIV present
cervix of the teachers of private schools in the city of
17
Experimental Pathology and Health Sciences
Patos. Methodology: We conducted a questionnaire with
-
cited. Conclusions: The research pointed to a good level
uting to ongoing guidance and conscious of the importance of prevention and early detection through dialogue
with society.
Keywords: Breast cancer, cervical cancer, Teachers,
Private Schools
CPE-1396
A quick method for diagnosis of mycotic infections
of the eye by using lactophenol cotton blues stain: A
case report
Marsden, A1; Leite, E1; Magalhães, O.M1; Souza Motta,
Massa, D; Gaioso, F2; Neto, G2.
1. Laboratório de Micologia Médica, Centro de Ciências BiológicasUniversidade Federal de Pernambuco.
2. Hospital das Clínicas, Universidade Federal de Pernambuco.
illness where fungi are involved. There are; Mycotic Keratithat occurs due to a dissemination of the systemic infectorial examination for fungus infections. Methodology: After scrapping the cornea the material was prepared for direct smear. In the preparation technique, instead of using
potassium hydroxide mount, we tried lactophenol cotton
many fungal structures. The most of hiphae and mycelium
and other fungi cells presented so typical and very well
stained. Conclusion: According to our method, the use of
result than potassium hydroxide in this case. In this case
we isolate Fusarium equiseti and the patient was treated
cure after two months of treatment.
Keywords: Mycotic Keratitis. Fusariosis, Mycotic ulcers
CPE-1402
Intestinal villi changes in experimental administration of semicarbazide
1
3
, Pedro Marques2
, Fernan1
do Capela e Silva3, Rodrigo Farinha1
1. Experimental Pathology Service, Faculty of Medicine, University
of Coimbra
2. Faculty of Pharmacy, University of Coimbra
3. Mediterranean agronomical and environmental sciences Institute,
University of Évora
conducted this study to evaluate morphologic changes of
the small intestine related to this disease. Thirty animals
18
the complete necropsy was performed. The small intestine
was collected for histopathological processing and analysis.
Pictures of routine stained slides were taken and the small
intestinal villi length measured, using the software ImageJ.
- VB) using the average and standard deviation, for the
control group. The test groups were compared with the
control one. In the control group the percentage of each
class was: 2 VS; 16 S; 64 M; 16 B; 2 VB. In the group II the
percentage of each class was: 87 VS; 13 S; 6 M; 1 B; 0 VB.
In the group III the percentage of each class was: 93 VS;
30 S; 4 M; 0 B; 0 VB.
collagen synthesis is necessary to maintain the intestinal
villi length.
CPE-1402a
Intestinal connective compartment in experimental
administration of a carcinogen
1
, Karen Cavalcanti2, Pedro Marques3, Rodri1
go Farinha1, Ivone Souza2
1. Experimental Pathology Service, Faculty of Medicine, University
of Coimbra
2. University of Pernambuco - Recife
3. Faculty of Pharmacy, University of Coimbra
cide mainly used on rice and cotton cultures as insecticide.
Although its short persistence in the environment the pes-
and respiratory tracts. This study was conducted to evaluate morphologic changes of the small intestine related to
in two groups, with 10 animals each one. The group I was
a control group kept with no manipulation for 28 weeks.
mals were maintained with no manipulation until the end
in all animals. Pictures of routine stained slides were taken
and the intestine connective compartment was measured,
of intestinal wall thickness in the test group. Considering
condition there is a fragility of the intestinal wall.
CPE-1403
Histomorphometric study of effect of different doses
of olanzapine on bone tissue of mice
Brito, V.C.1; Melo, R.C.B.2; Amorim, M.J.A.A.L1; Oliveira,
B.D.R.3
1. Animal Morphology and Physiology Department of Federal Rural University of Pernambuco, Brazil
2011 5 (2)
2. Department of Veterinary Medicine of Federal Rural University
of Pernambuco, Brazil
3. Caruaruense Association of Higher Education, Brazil
Background: Atypical antipsychotics (eg. Olanzapine)
sult, serotonin receptor antagonism promotes reduction
of hiperprolactinemia, the main factor associated with
adult mice, males were randomly divided into three groups
according to the experimental dose used: Control Group
etine groups is also smaller than the saline groups with
on the development of skeletal muscle tissue.
Keywords: Fluoxetine; Skeletal Muscle, Muscle Fiber
CPE-1404
Alpha-Pinene: Antioxidant And Hemolytic Effects
was performed within 30 days with intraperitoneal application of the drug. At the end of the treatment, mice
were weighed. Then the animals were anesthetized via
intramuscular and performed the dissection of the lower
H. L. F.3; Diniz, M. F. F. M.1; Souza, M. F. V.1;
1Departamento de Ciências Farmacêuticas, CCS, UFPB/ Brazil.
2Programa de Pós-Graduação em Produtos Naturais e Sintéticos
Bioativos, CCS, UFPB/ Brazil.
3Departamento de Biologia Molecular, CCEN, UFPB/ Brazil.
the thickness of the proximal and distal epiphysis and diaphysis thickness were measured with the aid of a digital
sent for histological processing. Results: Group 2 had a re-
fungi species, an example of them is the alpha-pinene
potential oxidant, antioxidant and hemolytic using human
erythrocytes as an experimental model. Methodology:
the control group. The length and thickness of the proximal and distal epiphyses showed no statistical differences.
when comparing experimental groups to each other, group
animals.
Keywords: Olanzapine, an atypical antipsychotic,
bone tissue
was investigated in vitro using human erythrocytes under
els were measured spectrophotometrically. And the hemolytic assay was performed with human erythrocytes type
positive control was erythrocyte suspension and 100 µL
CPE-1403a
phometric analysis of the skeletal muscle
2
; Oliveira, B.D.R. 3; Moraes,
Brito, V.C.1
4
S.R.A.
1- Animal Morphology and Physiology Department of Federal Rural University of Pernambuco, Brazil
2 – Physiotherapy Department of Federal University of Pernambuco, Brazil
3 - Caruaruense Association of Higher Education, Brazil
4 – Anatomy Department of Federal University of Pernambuco,
Brazil
Background: Pharmacological manipulation using drugs
which interfere with the serotonergic system, including seAnalyzed the effects of neonatal serotonergic manipulaclei and the cross section area. Materials and methods: 24
male rats, Wistar, of 30 and 90 days old were used. They
weight). The animals were divided into Saline Group (GS30 and GS-90) and Fluoxetine Group (GF-30 and GF-90).
the Ethical Committee for Research with Human Specieffect on any concentrations. When human erythrocytes
-
have antioxidant potential. The monoterpene induced a
exposed that alpha-pinene did not show oxidant or antioxidant action. Also, it was demonstrated low hemolytic
activity. However more studies are needed to investigate
the toxicity of this monoterpene.
Keywords: Monoterpene; Alpha-pinene; Cytotoxicity
CPE-1404a
Beta-Pinene: Antioxidant And Hemolytic Effects
H. L. F.3; Diniz, M. F. F. M.1; Souza, M. F. V.1;
1. Departamento de Ciências Farmacêuticas, CCS, UFPB/ Brazil.
2Programa de Pós-Graduação em Produtos Naturais e Sintéticos
Bioativos, CCS, UFPB/ Brazil.
3Departamento de Biologia Molecular, CCEN, UFPB/ Brazil.
19
Experimental Pathology and Health Sciences
(81.4±5.5, 53.4±11.5, 27.9±10.7, respectively) vs contential oxidant, antioxidant and hemolytic. Methodology:
phase. In the second phase also decreased the licking time
in a dose dependent 50 (54.5±12.0), 100 (17.6±17.6) and
200 (0.0±0.0) seconds vs. control (141.5±34.4) and is similar to morphine (33.7±13.4). Conclusion: Therefore, it is
in vitro using human erythrocytes under oxidative stress
-
-
And the hemolytic assay was performed with human eryth-
nisms involved.
Keywords: Farnesol, antinociceptive activity, CNS
sis) and the positive control was erythrocyte suspension
CPE-1406
Is Uncaria tomentosa (cat’s claw) a safe natural product to population?
the Ethical Committee for Research with Human Specieffect on any concentrations. When human erythrocytes
-
I. M.2.
1. Laboratório de Farmacologia e Toxicologia, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, FMVZ
- USP, SP.
2. Instituto de Ciências Ambientais, Químicas e Farmacêuticas da
Universidade Federal de São Paulo - UNIFESP - Campus Diadema, SP.
is a vine that grows in the rainforests of tropical countries
have antioxidant potential. The monoterpene induced a
immune stimulatory effect. Despite of many studies re-
adage: “if it is natural, it is not danger”, few studies were
however, hemolytic activity demonstrated in high concentrations.
Keywords: Monoterpene; beta-pinene; Cytotoxicity
-
CPE-1405
Evaluation Of Antinociceptive Activity Of Farnesol:
A Chemical Component Of Some Essential Oils
Pereira, W.B.1, Benedito, R.B.1, Junior, W.M.D.O.1, Torres, P.A.1, Morais, L.C.S.L.D.1, Sousa, D.P.D.2, Diniz,
M.F.F.M.1, Almeida, R.N.1
1. Laboratório de Tecnologia Farmacêutica - UFPB, Brasil
2. Departamento de Fisiologia – UFS, Brasil
Background: Essential oils represent a promising source of
cal activities, such as anxiolytic, anticonvulsant and antinociceptive. The farnesol, an isoprenoid alcohol, is present in
study investigated the antinociceptive activity of farnesol
(FAR) in animal models. Methodology: Male Swiss mice
for 28 days. Every other day food consumption (FC) and
weights, cellularity and histology). Results: Despite of no
statistical differences in FC among the groups, it was veri-
Conclusion: At the present moment, this preliminary study
rameters evaluated. However, it is important to highlight
-
after treatment with farnesol, the animals were treated with
injected under the right paw surface, 30 min after the drugs
administration. And it was recorded the lick time (s) of the
terphase), the lick time was evaluated for 15 min (second
phase). Results: Animals treated with three doses of FAR
(0.0±0.0) (0.0±0,0), respectively, vs control (18.1±4.6). It
showed very similar results with morphine (0.0 ± 0.0). Animals treated with FAR showed reduced paw licking time
20
evaluate the safety of this plant to population.
Keywords: Uncaria tomentosa, toxicity, immunotoxic
CPE-1407
Risk factors associated with the health of pregnant
women attended in a maternity hospital in Recife
Lima, B.L.F. 1; Ferreira S.R.R. 1; Sousa, G.M.G 1; Anjos,
F.B.R. 1
1. Federal University of Pernambuco, Brazil.
pregnancy. Among the factors that interfere with the normal evolution of the pregnancy include: age, parity, envi-
2011 5 (2)
volved in maternal health. Methodology: The methodology was a cross-sectional with semi-direct questionnaires
applied in the Maternity Professor Bandeira Filho, during
the years of 2006 to 2007. The following data was collected: family income, education, food consumed and men-
-
the interview, signed a consent form, aware of its contents.
Results: The level of education of the pregnant women
did not informed. The monthly income of interviewees
-
with other groups. Conclusion: These results suggest that
DEP and the treatment with Methanol that represent the
less drastic extraction of organic compounds and metals
in Golgi.
Keywords: Explant, Trachea, Pollution
CPE-1408a
als and fats in general. Conclusions: All these data indicate
aiming at prevention and health promotion. To this end,
order to raise awareness of pregnant women and so reduce
some risk factors associated with the health of this group.
Keywords: risk, health, pregnant
CPE-1408
Toxicity of fractions of particulate matter from diesel
exhaust on tracheal explants
Choqueta de Toledo3, Milton de Arruda Martins3, Adriano Alencar4, Thaís Mauad1, Luiz Fernando Ferraz Silva¹,
Paulo Hilário Nascimento Saldiva1 and Mariangela Macchione1
1Laboratório de Poluição Atmosférica Experimental do Departamento de Patologia da Faculdade de Medicina da Universidade de
São Paulo. [email protected]
2Laboratório de Terapeutica Experimental da Clínica Médica da
Faculdade de Medicina da Universidade de São Paulo.
3Biotério Central da Faculdade de Medicina da Universidade de
São Paulo.
4Laboratório de Micro-reologia e Fisiologia Molecular do Instituto
de Física da Universidade de São Paulo.
Background: Diesel exhaust particles (DEP) have wide
niloticus from Guarapiranga Reservoir, São PauloBrazil
Michelly R. R. Silva4, Eduardo O. Villares4, Dolores H.
R.F. Rivero4; Maria J. T. Ranzani-Paiva5; Mariângela Macchione¹
1- Faculdade de Medicina – Universidade de São Paulo - São
Paulo – SP.
2- Universidade Estadual Paulista – Campus Litoral Paulista –
São Vicente, SP.
3- LABOMAR - Universidade Federal do Ceará – Fortaleza,
Ceará
4- Universidade Paulista – UNIP – Campus Paraíso – São Paulo,
SP.
5- Instituto de Pesca – São Paulo, SP.
programs as a tool to estimate the degradation of aquatic
indicator of physiological effects of water contamination
lected with heparinized syringes. The frequency of micronuclei and percentage of differential leukocytes was
wald-Giens. Liver were removed for elemental analysis in
energy dispersive X-ray spectrometer (EDX). The Cu, Zn,
evaluate the toxicity in tracheal explants of DEP: a) pure
als), c) Hexane (remove organic compounds of low polarity) and d) Methanol (remove organic compounds of
-
moved and kept in plates (DMEM-F12 and supplements)
elements, Fe and Se, were lower in liver of GR. Concluelements in environmental toxicology, such as Hg, Cd and
sections and stained: periodic acid Schiff (PAS) to stain
neutral mucus in red and Alcian Blue (AB) to stain acidic
essential elements such as Fe and Se. Fe is a constituent
of several enzymes related to oxidative processes and cell
were evaluated: acid mucus (AB+), neutral mucus (PAS+),
activity of glutathione peroxidase, an enzyme constituent
of the antioxidant defense system found in the liver. Ac21
Experimental Pathology and Health Sciences
tration of elements were sensitive to diagnose differences
and Methods: Larvae of A. aegypti were exposed to the
environmental quality.
Keywords: Pollution, Biomarkers, Bioacumulation
ried out in duplicate for each concentration. Larvae were
CPE-1409
Protective effect of Indican from leaves of Indigofera
suffruticosa on liver of mice bearing sarcoma 180
Nascimento, D.K.D.1; Lima, I.R.1; Oliveira, Y.D.F1; Silva,
I.B.1;Vieira, J.R.C.1; Leite, S.P.1
1. Departamento de Histologia e Embriologia- CCB/UFPE, Brazil
Background: Indigofera suffruticosa is a plant of the
velopmental stages of larvae were determined at the start
of the experiment (0 h) and 24, 48, and 72 h thereafter, and
dican isolated from I. suffruticosa showed that this com-
The aqueous extract of I. suffruticosa in concentration of
are important tools to detect changes histophysiological
histologically and morphometrically hepatic sinusoid capfrom leaves of I. suffruticosa. Materials and Methods:
a stereomicroscope (1.2 x). Morphologic Study: Control
graphed using a digital video camera (Leica) connected to
A. aegypti that remained in L2 instar and showed disruption on the peritrophic envelope (PE) structure, consequently resulting in a discontinued underlying epithelium,
increased gut lumen, and segments with hypertrophic aspects in comparison with control larvae. Conclusion: The
developmental delay is directly dependent of morphological changes that occur when the larvae are growing in con-
days old, weighing from 30 to 40g were divided in three
180 and G2 without sarcoma 180. G1 and G2 - Controls
with saline; G3 - treated with Indican. It was injected into
the right axillary 0.3 mL (around 3x106 cells) the ascitic
form of Sarcoma 180 in groups G1 and G3. After 48
hours of tumor implantation, the treatment was initiated
of the histological preparations and the photomicrographs
were realized using the optical microscope with camera attached. Morphometric evaluation of the diameter of hepatic sinusoid capillaries was performed using the software
sults: In G3 group the diameter of the hepatic sinusoid
compound Indican from leaves of I. suffruticosa showed
a low order of toxicity on hepatic sinusoid capillaries of
tive effect.
Keywords: Indigofera suffruticosa; sarcoma 180; hepatic sinusoid capilaries
CPE-1409a
Morphological observation of Aedes aegypti larvae
treated with extract of Indigofera suffruticosa
Nascimento, D.K.D.1; Lima, I.R.1; Oliveira, Y.D.F.1; Silva,
I.B.1; Vieira, J.R.C.1; Leite, S.P.1
1. Departamento de Histologia e Embriologia- CCB/UFPE, Brazil
Background: The mosquito Aedes aegypti is a vector that
promotes the dissemination of four serotypes of den-
Keywords: Indigofera suffruticosa; Aedes aegypti;
larvae
CPE-1411
Nephrogenic Systemic Fibrosis
Silva, S.R.1; Pereira, B.D.S.1; Santos, A.H.1; Nunes, M. K.
S 1,2; Silva, B.D.F.1; Cardoso, H.M1; Machado, W.G.S.1;
Costa, L.A .1; Carvalho,V.A1,3.
1. Santa Emilia de Rodat Faculty, student of Biomedicine, Brazil
2. Federal University of Paraiba, student of Nursing, Brazil.
3. Santa Emília de Rodat, College. Valderi Alves de Carvalho Biomedicine professor . João Pessoa, PB, Brazil.
and rare disease which mainly affects the skin of renal
muscle contractures around joints. The distal areas are
face is rarely never compromised. It may affect the skeletal
muscles and internal organs such as diaphragm, lungs, and
on a systematic review of the medical literature. Method-
with FSN. However, patients affected with the disease have
mation, including increased levels of C -reactive protein,
serum ferritin, erythrocyte sedimentation rate, and a reused in MRI that seems to only affect patients with advanced renal dysfunction. Some studies have shown an in-
-
-
morphological changes on larvae of A. aegypti treated
with aqueous extract of leaves of I. suffruticosa. Materials
tive treatment or prevention. However, recent studies have
22
2011 5 (2)
shown strong association with gadolinium. Experts rec-
and NSF, for security reasons.
kidney failure
CPE-1411a
The treatment of the chronic Chagas cardiopathy
through stem cells transplant from the bone marrow
Silva, S.R.1; Pereira, B.D.S.1; Santos, A.H.1; Nunes, M. K.
S 1,2; Santos, E.P.1; Silva, B.D.F.1; Cardoso, H.M1; Machado, W.G.S.1; Costa, L.A .1; Carvalho,V.A1,3.
1. Santa Emilia de Rodat Faculty, student of Biomedicine, Brazil
2. Federal University of Paraiba, student of Nursing, Brazil.
3. Santa Emília de Rodat, College. Valderi Alves de Carvalho Biomedicine professor . João Pessoa, PB, Brazil.
opathy which affects millions of people in Latin America.
Studies with experimental models of IC due the DC indicate that the transplant of mononuclear cells from the
tential of these cells in specialized types, like cardiomyomyocardial in patients with heart failure from Chagas etiology and its response as therapy, in a literature review in
an exploratory and analytical way. Methodology: The pres-
these ones, three have involved the TCMO in patients
with chronic Chagas cardiopathy and one has involved the
same transplant in animals with Chagas cardiopathy. There
were not any complications directly related to the proce-
Gadelha, C. A. A.1; Santi-Gadelha, T.
1. Laboratory of Biochemistry, Genetics and Radiobiology – BioGer/UFPB, Brazil
2. Student of the Graduate Program in Nutrition Science – UFPB,
Brazil
3. Student of the Graduate Program in Biochemistry – UFC, Brazil
4. Student of the Graduate Program Bioactive Natural and Synthetic Products – UFPB, Brazil
Introduction: Lectins are a group of (glyco)proteins that
of this study is to assess the oxidant and antioxidant acWe used Triton X-100 to promote hemolysis and exposure
a spectrophotometer at a wavelength of 630nm and 540
serving whether AEL protects the erythrocytes against the
done with a Pasteur pipette, due to the need for oxygen
Phenylhydrazines. In erythrocytes treated with AEL, the
AEL showing that acts as antioxidant compound decreas-
-
lectin showed little relation to oxidant Phenylhydrazines
tion of this agent.
Keywords: Lectin; Abelmoschus esculentus; Oxidation
markers or neuro-hormonal. Other study involving ani-
CPE-1412a
Isolation and Characterization of a Lectin from Seeds
of Mucuna pruriens
cate that the therapy with stem cells has a promising future, the initial results of the response to the therapy with
1. Laboratory of Biochemistry, Genetics and Radiobiology – BioGer/UFPB, Brazil
2. Student of the Graduate Program in Biochemistry – UFC, Brazil
3. Student of the Graduate Program in Nutrition Science – UFPB,
Brazil
peutic effectiveness of the mentioned procedure.
Keywords: Chagas cardiopathy, stem cells transplant,
cell therapy
CPE-1412
Abelmoschus esculentus lectin reduces oxidant action of phenylhydrazine
Gomes, V. M.1; E-Lacerda, R. R.1; Caluete, M. E. E.2;
Leite, J. F. M. 3; Pessoa, H. L. F.1; Gonçalves, G. F.4;
-
a lectin from the seeds of Mucuna pruriens. Methodolwhich were tested for the presence of hemagglutinating
23
Experimental Pathology and Health Sciences
chromatographed on a column of molecular exclusion
Sephadex G-100. All proteins were eluted protein disity and resistance to changes in pH was determined using
Keywords: Sterculia foetida; Pharmacology; Protein
CPE-1412c
seeds of Clitoria fairchildiana
resistance to proteolytic enzymes was determined using
chelating agent was determined using EDTA. Beta-mercaptoethanol and urea were used to determine the effect
of reducing agents, as well as the metaperiodato sodium
was used to verify the action of oxidizing agent. In all experiments, the hemagglutinating activity was determined
1. Laboratory of Biologically Actives Molecules - Federal University
of Ceará, Brazil
2. Laboratory of Biochemistry, Genetics and Radiobiology - Federal
University of Paraíba, Brazil
University of Ceará, Brazil
chromatography. Its molecular mass was estimated at 116
-
change its structure, as well as any reducing agent. The
from seeds of Clitoria fairchildiana. Methodology: Paw
hemagglutinating activity. Conclusion: It was isolated and
partially characterized a lectin from the seeds of Mucuna
pruriens approximately 116 kDa. More studies are under-
-
Keywords: Lectin; Mucuna pruriens; characterization
CPE-1412b
Prospecting Biochemistry and Pharmacology of Protein Fractions of Sterculia foetida
were calculated using the trapezoid method and expressed
-
1. Laboratory of Biochemistry, Genetics and Radiobiology – BioGer/UFPB, Brazil
2. Student of the Graduate Program in Biochemistry – UFC, Brazil
3. Student of the Graduate Program in Nutrition Science – UFPB,
Brazil
Background: Currently the plants are still an unknown and
-
spectrophotometry (A600nm). Peritonitis model: Lectin
and differential leukocyte counts. Results: The lectin-like
Conclusion: The lectin-like from C. fairchildiana presents
Sterculia foetida. Methodology: The seed meal has prochildiana
of type A, B and O, using the chemical Hemolyzing Trius, causing you to read the results in a spectrophotometer
glutelin showed hemagglutinating activity. All fractions
aeruginosa, Escherichia coli and Staphylococcus aureu.
By SDS-PAGE showed the variety of protein fractions
CPE-1412
Abelmoschus esculentus lectin reduces oxidant action of phenylhydrazine
Gomes, V. M.1; E-Lacerda, R. R.1; Caluete, M. E. E.2;
Leite, J. F. M. 3; Pessoa, H. L. F.1; Gonçalves, G. F.4;
Gadelha, C. A. A.1; Santi-Gadelha, T.
1. Laboratory of Biochemistry, Genetics and Radiobiology – BioGer/UFPB, Brazil
2. Program in Nutrition Science – UFPB, Brazil
3. Program in Biochemistry – UFC, Brazil
4. Program Bioactive Natural and Synthetic Products – UFPB,
Brazil
Introduction: Lectins are a group of (glyco)proteins that
in seeds of Sterculia foetida. All protein fractions of the
logical use of its constituents.
24
of this study is to assess the oxidant and antioxidant ac-
2011 5 (2)
We used Triton X-100 to promote hemolysis and exposure
a spectrophotometer at a wavelength of 630nm and 540
serving whether AEL protects the erythrocytes against the
done with a Pasteur pipette, due to the need for oxygen
ity. Conclusion: It was isolated and partially characterized
a lectin from the seeds of Mucuna pruriens approximately
logical function.
Keywords: Lectin; Mucuna pruriens; characterization
CPE-1412b
Prospecting Biochemistry and Pharmacology of Protein Fractions of Sterculia foetida
Gadelha, C. A. A.¹; Santi-Gadelha, T.¹
1. Laboratory of Biochemistry, Genetics and Radiobiology – BioGer/UFPB, Brazil
2. Program in Biochemistry – UFC, Brazil
3. Program in Nutrition Science – UFPB, Brazil
Background: Currently the plants are still an unknown and
Phenylhydrazines. In erythrocytes treated with AEL, the
-
AEL showing that acts as antioxidant compound decreasSterculia foetida. Methodology: The seed meal has prolectin showed little relation to oxidant Phenylhydrazines
tion of this agent.
Keywords: Lectin; Abelmoschus esculentus; Oxidation
of type A, B and O, using the chemical Hemolyzing Tri-
CPE-1412a
Isolation and Characterization of a Lectin from Seeds
of Mucuna pruriens
us, causing you to read the results in a spectrophotometer
-
glutelin showed hemagglutinating activity. All fractions
1. Laboratory of Biochemistry, Genetics and Radiobiology – BioGer/UFPB, Brazil
2. Program in Biochemistry – UFC, Brazil
3. Program in Nutrition Science – UFPB, Brazil
-
aeruginosa, Escherichia coli and Staphylococcus aureu.
By SDS-PAGE showed the variety of protein fractions
in seeds of Sterculia foetida. All protein fractions of the
lectin from the seeds of Mucuna pruriens. Methodology:
were tested for the presence of hemagglutinating activity
tographed on a column of molecular exclusion Sephadex
G-100. All proteins were eluted protein displayed in their
logical use of its constituents.
Keywords: Sterculia foetida; Pharmacology; Protein
CPE-1412c
seeds of Clitoria fairchildiana
pH, respectively. The presence of resistance to proteolytic
melain and papain. The effect of chelating agent was determined using EDTA. Beta-mercaptoethanol and urea
were used to determine the effect of reducing agents, as
well as the metaperiodato sodium was used to verify the
action of oxidizing agent. In all experiments, the hem-
1. Laboratory of Biologically Actives Molecules - Federal University
of Ceará, Brazil
2. Laboratory of Biochemistry, Genetics and Radiobiology - Federal
University of Paraíba, Brazil
University of Ceará, Brazil
hemagglutinating. Results: A lectin was isolated from the
Its molecular mass was estimated at 116 kDa. This protein
well as any reducing agent. The presence of EDTA and
ity in the lectin-like from seeds of Clitoria fairchildiana.
25
Experimental Pathology and Health Sciences
Methodology: Paw edema model: C. fairchildiana (0.015,
ic
volume (mL) in a certain time interval after the stimulus
-
CPE-1413a
Behavioral assessment in wistar rats exposed to
chronic treatment with different doses of ethanolic
Ramalho, J.A.1; Lira, A.B1.; Dias, G.E.N.1; Alves,M.F.1;
-
L.S.N.1; Diniz, M.F.F.M.1; Medeiros, I.A1.
1. Laboratório de Tecnologia Farmacêutica, Universidade Federal
da Paraíba, Brazil
for total and differential leukocyte counts. Results: The lecclinical toxicological study the ethanolic extract of Dioclea
Conclusion: The lectin-like from C. fairchildiana presents
-
the animals were realized fortnightly, through the open
ticipated in the test for 3 minutes, for the evaluation of its
childiana
CPE-1413
Assessment biochemical parameters of wistar rats towards chronic treatment with different doses of etha-
sexes the results were compared with the control group. In
Ramalho, J.A.1; Lira, A.B1; Oliveira,K.M.D1; Dias,
malho, L.S.N.1; Medeiros,I.A1; Diniz,M.F.F.M.1
1. Laboratório de Tecnologia Farmacêutica, Universidade Federal
da Paraíba, Brazil
-
lance (ANVISA), the ethanolic extract of Dioclea grandi-
extract. Conclusions: Throughout the experiment showed
these effects.
days. In the end of the study, the animals were euthanized
-
evation of aspartate aminotraferase and lactic dehydrogenase in rats treated at all doses. Conclusions: Throughout
the experiment, showed signs of chronic toxicity, mainly
26
CPE-1414
Morphometric analysis of the prostate in experimental DMBA administration
Gustavo Barandas1, Eduardo Costa2, Felipe Pereira2, Rodrigo Farinha1
1. Experimental Pathology Service, Faculty of Medicine, University
of Coimbra
2. Pharmacy Faculty, University of Coimbra
nosuppressive action. This compound is found in cigarette
engines. The present study aims to evaluate the action of
DMBA on prostate using morphological parameters and
morphometric analysis.
quarantine period of one week. After that, they were ran-
2011 5 (2)
food handlers in a state nursery in the city of João Pessoa
tum. At the end of the assay time, all animals were ethically
killed and the complete necropsy performed. Fragments
of prostate were collected for routine histopathological
processing and examination. Pictures of routine staining
slides were taken and the measurement of its follicles areas
performed using the software ImageJ. The frequencies dis-
samples, including: 38 children and 25 adults. During the
phase consisted of fecal examinations of the samples, using the methods of Hoffman and Faust. Later, it was performed the treatment of infected individuals. Results: Af-
These results allow us to think that any change induced
CPE-1414a
Morphometric analysis of the thyroid in experimental
DMBA administration
Gustavo Barandas1, Magali Jorge2, Felipe Pereira2, Silvério
ter the analysis of the results it was noticed a high percentage of infected individuals for more than one species of
parasite, particularly those from fecal-oral transmission.
Keywords: multiparasitism, prevalência, transmission
1
1. Experimental Pathology Service, Faculty of Medicine, University
of Coimbra
2. Pharmacy Faculty, University of Coimbra
mune system and has a carcinogenic action. The human
exposure to DMBA is linked to several life events, such as
smoking and exposure to atmospheres with exhaust gases
CPE-1415a
Evaluation Of Antinociceptive Activity Of The
Monoterpene Linalool Oxide In Mice
Arruda, P.A.1, Souto-Maior, F. N.1,2, Salgado, P.R.R.1,
W.B.1, Morais, L.C.S.L.1, Almeida, R.N.1
1. Laboratório de Tecnologia Farmacêutica - UFPB, Brasil
2. Universidade Federal de Campina Grande, Brasil
Background: Linalool oxide (OXL) is a monocyclic alcohol
aims to evaluate the action of DMBA on thyroid using
morphological parameters and morphometric analysis.
reactions such as degradation product of linalool (Demytquarantine period of one week. After that, they were rantum. At the end of the assay time, all animals were ethically
killed and the complete necropsy performed. Fragments
of thyroid were collected for routine histopathological
processing and examination. Pictures of routine staining
slides were taken and the measurement of its follicles areas performed using the software ImageJ. The frequencies
characterized.
gration of the graph with more cases of larger follicles in
the test group.
These results allow us to conclude that thyroid is affected
experimental models. Methodology: Male Swiss mice, 25
to 35 grams, approximately 3 months, were divided in 5
min. after administration, the animals received 20 µL of a
surface. Then, it was recorded the lick time (s) of the inthis same parameter was evaluated for 15 min (second
phase). In the acetic acid induced writhing test ,thirty min
after the administration, the animals received acetic acid
at doses of 100 and 150 compared to the control group. In
at three doses (50, 100 and 150) compared to the control
follicles areas.
decrease in latency at doses of 100 and 150 compared to
CPE-1415
Prevallence Of Multiple Parasites In Children And
Food Handlers In A State Nursery In The City Of
João Pessoa - Pb
Torres, P.A.1, Moura, J.A.P.1, Antunes, P.S.1, Martins, I.S.1,
Rodrigues, D.A.1, Cardoso, B.C.1, Vieira, L. M.1, Rocha,
1. Universidade Federal da Paraíba, Brasil
2. Departamento de Ciências Farmacêuticas – UFPB, Brasil
Background: The enteroparasite infections are still responmine the prevalence of multiple parasites in children and
compared to the control group. Conclusions: These results
showed antinociceptive activity demonstrated in methodologies in vivo for analgesia. It is necessary others studies
fects.
Keywords: CNS, Linalool Oxide, Antinociceptive
CPE-1416
Ibuprofen nanoparticles for oral delivery: characterization and in vivo toxicity evaluation
1,
Fernandes,
Figueiredo, I.V.2,5, Veiga, F.2,5
C.3, Candeias, S.4, Reis, C.3
1. Institute of Experimental Pathology, Faculty of Medicine - Uni27
Experimental Pathology and Health Sciences
versity of Coimbra, Portugal
2. Faculty of Pharmacy, University of Coimbra, Portugal
3. CBIOS – Laboratory of Nanoscience and Biomedical Nanotechnology (LNBN), Universidade Lusófona de Humanidades e
Tecnologias, Portugal
4. CBIOS – Nutrition and Analitical Methods Unit (UNMA),
Universidade Lusófona de Humanidades e Tecnologias, Portugal
5. Center for Pharmaceutical Studies (CEF), Faculty of Pharmacy
– University of Coimbra, Portugal
tory drug (NSAID) widely used as analgesic and antipyret-
prepared according to the traditional Peruvian medicine.
and TLC analyses. We used the carrageenan-induced paw
edema test to assess the effect of the PACs rich fraction in
test was used to evaluate in vivo central analgesic activity
and writhing test was used to evaluate in vivo peripheral
-
spectives in pharmacology, it is intended, using this area,
fen as a model drug. Methodology: Polylactic acid (PLA)
-
ments were collected from various organs and tissues. All
for positive control group of edema reduction. The results
pain reduction. Although, results show that this fraction
ing that the analgesic activity is related to the nociception
tory process.
CPE-1416b
activities of Agrimonia eupatoria L. on in vivo models.
Santos T.1,2*, Ferreira J.1,2, Francisco V.1,2, Costa G.1,2,
281.1 ± 66.7 nm and zeta potential was -4.3 mV. The
not agglomerated. In respect to the analysis of histological results, there was a reduction of gastric toxicity when
successful in terms of the development and characterization of the formulation. Moreover, there was a reduction
M.T1,2, Castel-Branco M.M.1,2, Figueiredo I.V.1,2
1 Faculty of Pharmacy, University of Coimbra, Portugal.
2 Center for Pharmaceutical Studies (CEF), University of Coimbra, Portugal.
3 Faculty of Medicine, University of Coimbra, Portugal.
Introduction: Agrimonia eupatoria L. is used for the treatous studies indicate that the aqueous extract of Agrimonia
presents a prevalence of several phenolic compounds and
its ethyl-acetate fraction shows anti-oxidant activity and
now in progress, will test this strategy using other polyous extract of Agrimonia and its ethyl acetate fraction in
animal models and to control their toxicity. Methods: The
Keywords: Nanotechnology, Polymer, toxicology
CPE-1416a
ties of Uncaria tomentosa (Willd.) DC proanthocyanidins rich fraction on in vivo models
Ferreira J.1,2, Santos T.1,2, Francisco V.1,2, Costa G.1,2,
M.T.1,2, Castel-Branco, M.M.1,2, Figueiredo I.V.1,2
1 Faculty of Pharmacy, Universty of Coimbra, Portugal.
2 Center for Pharmaceutical Studies (CEF), University of Coimbra, Portugal.
3 Faculty of Medicine, University of Coimbra, Portugal.
The hot-plate test and writhing test were used to study,
respectively, the central and peripheral analgesic activities
and the formalin test to complement these results. The
tory activity. In all three tests we used test groups with two
concentrations (doses) of the sample. For the toxicological
assessment, we used the kidneys and livers of rats administrated with the samples. Results: The results for central
analgesia indicates no activity. With the writhing test, we
oedema reduction. Finally, the histologic analysis does
Discussion: These results prove the analgesic and antimatory and analgesic activities of a Proanthocyanidins rich
28
consequence of the activity, through several mechanisms,
of the phenolic compounds in Agrimonia. Also, there are
2011 5 (2)
-
no toxic effects of Agrimonia on liver or kidneys.
analgesic
CPE-1419
Histopathological description of 132 rat tumours
DMBA-induced
1
Calado, A1,2
1. Institute of Experimental Pathology, Faculty of Medicine - University of Coimbra, Portugal
2. Universitary School Vasco da Gama, Coimbra, Portugal
study was the assessment of systemic skeletal changes,
nous healing. Methodology: A total of 18 Wistar male
-
-
mary tumours in rats. There is a large consensus on histhe assayed period. Biochemical plasma analysis revealed
This study relates the tumour malignancy potencial with
tumors DMBA. Methodology: 48 Sprague-Dawley virgin
glucose, urea, alanine transaminase and ionized calcium,
while ionized phosphor values diminished from 15 days
Kg) at 50 days and ethically euthanized after 26 weeks. A
rats developed 132 mammary tumours, all collected and
processed for routine haematoxylin-eosin histopathology.
enomas and 127 carcinomas, 124 were invasive and 3 were
terns present was: 42 tumours with single pattern and 57
had 2 patterns, 30 had 3 patterns and 1 had 5 patterns. Two
groups, (1) 42 single pattern tumours and (2) 90 mixed pattern tumours, were considered for prevalence malignancy
potential on characteristics like histopathological pattern,
haemorrhage, tumour grading and stromal reaction. Reand papilar carcinoma in single patterns tumours and a
cinoma in mixed pattern tumours. Single pattern tumours
Necrosis, haemorrhage and stromal reaction were more
were of low and medium grade, respectively. Conclusions:
Mixed pattern tumours are more malignant than single
pattern ones. On future work is important to validate the
Keywords: DMBA, mammary tumours, histopathological evaluation
CPE-1419a
Assessment of skeletal systemic changes during local
bone tissue regeneration
Gomes PS1, Colaço B2, Pires2 MJ, Santos C3, Calado A4,5,
Carmezim MJ3, Fernandes MH1
1 – Laboratório de Farmacologia e Biocompatibilidade Celular –
Faculdade de Medicina Dentária, Universidade do Porto, Portugal
2 – CECAV. Universidade de Trás-os-Montes e Alto Douro.
Portugal.
3 – Instituto Politécnico de Setúbal, EST, Estefanilha, Setúbal,
Portugal.
4- Escola Universitária Vasco da Gama, Coimbra. Portugal.
5- Institute of Experimental Pathology, Faculty of Medicine - University of Coimbra, Portugal
generation remains an important challenge. Nonetheless
showed an inverse correlation with plasmatic ALP activity.
Biomechanical values diminished from 15 days (femurs and following increased at the 90 days time point (femurs
-
Keywords: Bone tissue; Bone regeneration; biomechanics.
CPE-1420
Protective Effects Of The Dipeptidyl Peptidase Iv Inhibitor Sitagliptin In The Blood-Retinal Barrier In A
Type 2 Diabetes Animal Model
Fernandes R 1, Gonçalves A 1, Leal E 2,3, Paiva A 4, Teixei1,3
ra-de-Lemos E 1,5, Teixeira F 1, Reis F 1
,
1
1. Pharmacology and Experimental Therapeutics, Institute of Biomedical Research in Light and Image (IBILI), Faculty of Medicine,
University of Coimbra, Coimbra, Portugall; 2. Center for Neuroscience and Cell Biology, University of Coimbra, Coimbra, Portugal;
3. Centre of Ophthalmology and Vision Sciences, IBILI, Faculty
of Medicine, University of Coimbra, Coimbra, Portugall; 4. Histocompatibility Center of Coimbra, Coimbra University Hospital,
Portugal; 5. ESAV, Polytechnic Institute of Viseu, Viseu, Portugal
attenuate some of the complications of this disease. The
(ZDF) rats.
Methods: ZDF rats at 20 weeks of age were treated with
29
Experimental Pathology and Health Sciences
Conclusions: The two analyzed materials have very distincNonetheless, in clinical practice these two materials are
used in similar applications. The morphostructural propcells present in peripheral circulation and endothelial proistry.
dardized research is recommended to explore the full poKeywords: bone graft, SEM, porosity
CPE-1423
Dental erosion with lactic acid: in vitro studies
levels of CD34+ cells in the peripheral circulation and
EPC to the retinal vessels.
Conclusions: Sitagliptin prevented nitrosative stress, inrat retinas.
Keywords: sitagliptin, blood brain barrier, diabetes
1. Experimental Pathology Service, University of Coimbra, Portugal
2. Pharmacology and Experimental Therapeutics, Institute of Biomedical Research in Light and Image (IBILI), University of Coimbra, Portugal
teria, namely the Streptococcus mutans, plays a very important role on the appearance of carious lesions on the
enamel surface. The initial stage caries is typically chardemineralization of the dental surface. On the other hand,
and knowing that after intense physical exercise lactic acid
teeth and other surrounding structures, we set out the hy-
CPE-1423
Morphostructural properties of two bone substitutes:
differences between a xenograft and a synthetic material
Guerra F.3
1. Experimental Patology Service, University of Coimbra, Portugal
2. Chemical Engineering Department, University of Coimbra, Portugal
3. Dentistry Department, University of Coimbra, Portugal
Background: Bone grafts are widely used in surgical procedures in Dentistry, particularly in Periodontology, Implantology, Endodontics and Oral Surgery. Although autoge-
alization in the form of dental caries and dental erosion.
Methodology: We exposed sixty eight third molars to four
solutions of lactic acid with distinct pH values (3.5; 4.5;
5.5 and 6.8) during different periods of time (24 hours, 3
days, 7 days and 14 days). After the exposure, we measured
water). Results: Only the solutions with pH values of 3.5
do not cause any kind of demineralization. It is not veri-
synthetic materials. A more future-oriented approach has
-
were exposed to the same solution and in the same condi-
-
investigation on the dissolution dynamics of hard tissues
(enamel and dentin) is needed in order to understand the
to analyze differences in what concerns morphostructural
Keywords: dental erosion, lactic acid, demineralization
CPE-1424
Metabolomic evaluation in a model for the Study of
mastigatory muscles
Rodrigo Farinha1, Marcolino Gomes2, Ludgero Tavares3,
granules are more irregular, with sharp-edge tips while
30
1 Experimental Pathology Institute, Faculty of Medicine, University
of Coimbra
2 Dental Medicine Area, Faculty of Medicine, University of Coimbra
3 Department of Biochemistry, Faculty of Sciences and Technology
and Center for Neurosciences, University of Coimbra
-
2011 5 (2)
tion, frequently associated with occlusal disarrangements.
The role of the different muscles and the degree of involvement of each one in working and non-working sides,
in this pathology, is not completely understood.
derstanding of the changes in the masseter muscle in an
experimental model of malocclusion.
to control, using paired Student “t” test. Conclusion:
EOLs reduced stomach fundus smooth muscle contracted
groups: the control group, with the animals kept in the
standard conditions and the test group, with a change of
studies pharmacological mechanisms.
Keywords: L. sidoides; contractility; stomach.
composite on the right upper teeth. After 2 weeks, all animals, were ethically killed and the complete necropsy performed. Fractions of masseter muscle were collected for
histopathological analysis and for high resolution magic
angle spinning NMR spectroscopy (HRMAS) in a 14.1
Tesla Varian spectrometer. The morphometric analysis
was performed using ImageJ software to measure the area
CPE-1427
Evaluation Of Toxicity Of The Hydroalcoholic Extract From The Stem Bark Of Pradosia Huberi On
Male Rats Vital Organs
-
F.L.P.1; Rocha, A.O.B1; Medeiros, A.A.N.2 ; Medeiros,
F.A.2; Diniz, M.F.F.M.3; Sá, R.C.S.4
1. PgPNSB/CCS/UFPB; 2. IEPA; 3. DCF/CCS/UFPB;
4. DFP/CCS/UFPB
phometric analysis presented a higher average area of the
medicine to treat gastritis and gastric ulcer. The presence
to the right muscle, where this area is smaller on test group.
statistical analysis demonstrated that aminoacids glutamate
and aspartate as well as lipids were incremented in the
right masseter of test group while choline was decreased.
investigate the toxicity of the hydroalcoholic extract of P.
Adult Wistar rats were divided into control and three treat-
glutamate and aspartate as well as lipids. This analysis algavage and once daily, for 63 days. The animals were killed
rotomy, the following organs were removed and weighed:
3D projection of principal components.
masseter muscles on working and non-working sides,
which allows the separation of the cases. Although with no
of the masseter muscle on this experimental model, with
weight of the kidney of the animals treated with PhHE
transverse cuts.
Keywords: Metabolomics, Morphometrics, Mastigatory muscle, Occlusal interference, Temporomandibular dysfunction
CPE-1426
Effect of Lippia sidoides essential oil on the stomach
smooth muscle contraction
Arruda, I.T.S.1 ; Medeiros, M. A. M. B.1 ; Nascimento,
; Souza, E.P.1
1. Laboratório de Bioquímica Molecular- DBM/UFPB, Brasil.
2. Laboratório de Tecnologia Farmacêutica – LTF/UFPB, Brasil.
3. Universidade Federal do Ceará – UFC, Brasil.
Introduction: Lippia sidoides, a medicinal plant popularly
known as rosemary pepper, presents thymol as the main
component of essential oil. Thymol affects smooth muscle
fects of essential oil of L. sidoides (EOLs) on the contractility of the stomach fundus. Methods: Stomach fundus of
the rats were dissected, sectioned and suspended in a tissue
cumulative doses of essential oil of L. sidoides (EOLs)
dissolved in DMSO in concentrations of 1, 3, 10, 30, 100
when compared to the control group (right 0.35 ± 0.01,
in the relative weight of these two groups, suggesting the
tion to the control group (398.1 ± 5.6 g). Conclusions:
These results suggest that the hydroalcoholic extract of P.
of rats.
Keywords: Pradosia huberi; toxicity; vital organs.
CPE-1427a
Stem Bark Of Pradosia Huberi On The HematologiF.L.P.1; Rocha, A.O.B1; Xavier, A.L.1; Pita, J.C.L.R.1; Medeiros, A.A.N.2 ; Diniz, M.F.F.M.3; Sá, R.C.S.4
1. PgPNSB/CCS/UFPB; 2. IEPA; 3. DCF/CCS/UFPB;
4. DFP/CCS/UFPB
azonian species, is popularly known as “casca-doce” and
used in the folk medicine for the treatment of gastritis.
ing vehicle. Data (mean ± standard error of mean) were
-
Evaluate hematological parameters of male Wistar rats under the effect of different doses of hydroalcoholic extract
31
Experimental Pathology and Health Sciences
which the animals received water and PhHE (T1 - 1.22,
(EDTA). The following hematological parameters were
CPE-1428a
Evaluation Of The Fertility Of Male Wistar Rats
Treated With The Hydroalcoholic Extract Of Pradosia Huberi
P.S.1; Rocha, A.O.B1; Medeiros, A.A.N.2 ; Medeiros, F.A.2;
Diniz, M.F.F.M.3; Sá, R.C.S.4
1. PgPNSB/CCS/UFPB; 2. IEPA; 3. DCF/CCS/UFPB;
4. DFP/CCS/UFPB
nities in the treatment of gastritis. Bioactive compounds
(41.30 ± 1.53), T1 (42.69 ± 1.40), T2 (42.32 ± 1.36), T3
(42.83 ± 1.15). The WBC and platelets also showed no
jectives: To investigate the effects of the PhHE on the
fertility of male rats mated with untreated females, which
later had pregnancy interrupted on 15th gestational day.
Methodology: Male rats were divided into control and
(7.21 ± 0.66), T1 (6.27 ± 0.5), T2 (5.57 ± 0.59), T3 (6.90
T1 (626.20 ± 55.39), T2 (703.80 ± 43.60), T3 (701.27 ±
24.45). Conclusions: The administration of the PhHE
days. Each rat was mated with two virgin females from the
57th to 63th day of treatment. The presence of sperm in
vaginal smear was considered indicative of insemination
(1th day of gestation). Pregnant females were monitored
Keywords: Pradosia huberi; rats; hematological parameters.
monitored throughout the gestational period (21 days) and
the other had pregnancy interrupted on the 15th day. The
occurrence of external malformations and the following
reproductive index were analyzed: mating, gestation, preimplantation loss, implantation and resorption. Results:
The administration of the extract did not interfere with
CPE-1428
Effect Of The Hydroalcoholic Extract Of Pradosia
P.S.1; Rocha, A.O.B1; Xavier, A.L.1; Pita, J.C.L.R.1; Medeiros, A.A.N.2 ; Diniz, M.F.F.M.3; Sá, R.C.S.4
1. PgPNSB/CCS/UFPB; 2. IEPA; 3. DCF/CCS/UFPB;
4. DFP/CCS/UFPB
for presence of sperm in the vaginal smear (mating in-
of PhHE, respectively. The gestatation, resorption indexes
the Amazon, used in folk medicine to treat gastritis. The
ogy: Adult Wistar rats were divided into control and three
-
These results indicate that the PhHE may impair fertility
due to increased pre-implantation losses and reduced implantation, suggesting the occurrence of mutagenic effects
on Wistar rats.
Keywords: Pradosia huberi; fertility; rats.
CPE-1430
Oesophagus morphometric analysis in experimental
administration of semicarbazide
Costa E1, Ramos A2, Capela-Silva F2, Marques P1,
for measurement of glucose, urea, acid uric, creatinine,
transaminase and aspartate transaminase. Results: The
(T3 - 2.7 ± 0.05). Although some parameters were high,
1.Faculdade de Farmácia da Universidade de Coimbra
2.Universidade de Évora
3.Faculdade de Medicina da Universidade de Coimbra, Serviço de
Patologia Experimental
pact on some populations with economic restrictions. This
tor of lathyrism. The aim of this study is to evaluate the
oesophagus morphologic changes in the administration
(1.3 ± 0.3). Conclusions: The administration of PhHE did
of the serum parameters measured in these animals.
Keywords: Pradosia huberi; rats; biochemical parameters.
32
-
2011 5 (2)
and the complete necropsy was performed. The fragments
collected in the necropsy were studied under light microscopy. The medium third oesophagus transversal cuts slides
lumen and the epithelial compartment areas. In the group
I the epithelial compartment has a value of 228.27 ± 38.74
µm2 and the lumen 245.59 ± 28.78 µm2. In the group II
the epithelial compartment has a value of 179.2 ± 42.69
µm2 and the lumen 118.25 ± 71.0 µm2. In the group III
the epithelial compartment has a value of 189.90 ± 65.33
µm2 and the lumen 100.66 ± 39.0 µm2. These results allow us to conclude that the lumen area of oesophagus is
water and Tween 80, the experimental groups, IM-3. In
Rota-rod test, the animals were treated and after 30, 60 and
120 minutes of administration were placed in the device.
utes. In the elevated plus maze test, exploration capacity
of entries and total time spent in open arms and closed
for 5 minutes. Results: In the Rota-rod test, there was not
vs. 179.3±0.7), 60 (50: 162.4±15.5; 100: 159.4±18.8; 200
changes were detected on the epithelium.
Keywords: Semicarbazide; Lathyrism; Oesophagus.
vs. 173.0±6.0) after treatment. In the elevated plus maze
CPE-1431
of entries in open arms (3.2±0.6, 2.2±0.8 and 2.6±1.0,
respectively vs. 2.5±0.8). The stay time in the open arms
Carvalho, A.S.1; Martins, I.S.1; Antunes, P.S.1; Sales,
I.R.P.1; Vasconcelos, W.P.1; Nascimento, R.F.1; Silva,
R.O.1; Oliveira, K.H.S.P.1; Araújo, I.K.M.1.; Freitas,F.I.S1
1. Departamento de Ciências Farmacêuticas – Universidade Federal
da Paraíba, Brasil
Background: With regard to parasites, although high rates
are most often detected in developing countries, little is
kg: 35.2±12.4 vs. 41.2±14.3). Conclusions: The IM-3 does
anxiolytic drugs, according to the elevated plus maze test.
Keywords: IM-3, derived imidazolidine, central nervous system
has own characteristics and transient standards of metaCidade Verde. Methodology: The records of 56 pregnant
soa - PB were analyzed, and from them were registered the
results of parasitological examinations performed during
pregnancy. Results: Given what was analyzed was found
CPE-1432a
Darier Disease (Dd) – Differential Diagnosis From The
Pathological Exam
Paulo, L.L.1; Silva-Netto, L.M.S.2; Sousa-Neto, F.F.2; Leite,
ga, R.M.1; Mangueira, V.M.1
1. Universidade Federal da Paraíba, João Pessoa-PB, Brasil.
2. Faculdade de Ciências Médicas da Paraíba, João Pessoa-PB,
Brasil.
Introduction: DD is a rare autosomal dominant genodermatosis of chronic course that presents changes in the
keratinization of the epidermis, mucous and phanero. It
-
-
the DD of the others acantholytic dermatoses, from the
pathological examination. Methods: It was made a clinical
infection in pregnant women, and this low prevalence is
them during pregnancy.
Keywords: Pregnants; parasites; immunology.
CPE-1432
Evaluation Of Locomotor And Anxiolytic Activity Of
Im-3
R.M.1; Monte, L.O.1; Souza, S.A.2; Almeida, R.N.1; Morais, L.C.S.L.1
1. Laboratório de Tecnologia Farmacêutica, Universidade Federal da
Paraíba, PB, Brasil.
2. Departamento de Química, Universidade Federal da Paraíba,
João Pessoa-PB, Brasil.
Introduction: The fact of IM-3 has a similar hydantoins
structure, known for its effects on the central nervous system (CNS), increase the intention to analyze the effects
using rodents. Methodology: In the Rota-rod and elevated
grams and divided into 8 groups. The administrations
and from this it was made the pathological exam. Results:
Originally appeared in the anterior face chest with gradual
worsens with sun exposure. The dermatological examination showed papules and plaques keratotic scaly, erythematous, in the anterior chest, cervical region, face and upper
epidermis with focus of hyperkeratosis, dyskeratotic kerasion: From these results it can has a differential diagnosis,
concluding that it was the DD. So, we can guide the patient
favors the non-appearance of secondary infections.
Keywords: DD, pathological exam, differential diagnosis
CPE-1433
The Pharmaceutical Care Exist? A Timely Analysis
Of An Integrated Unit Of Health In João Pessoa – Pb
Galvão, J.G.F.M.1; Antunes, P.S.1; Martins, I.S.1; Sales,
I.R.P.1; Vasconcelos, W.P.1; Nascimento, R.F.1; Silva,
R.O.1; Oliveira, K.H.S.P.1; Araújo, I.K.M.1.; Freitas,F.I.S1
1. Departamento de Ciências Farmacêuticas – Universidade Federal
33
Experimental Pathology and Health Sciences
da Paraíba, Brasil
Background: Pharmaceutical care is an interdisciplinary
tem, involves the supply of drugs provided to the user to
ensure the rational use of medicines. The pharmacist is
166.5±3.6 vs. 179.1±0.4) after treatment. Conclusions:
at improving access and promoting rational use of drugs,
for the full development of pharmaceutical care. But in
our reality, the pharmacist is not inserted in the process of
primary health care, not only causing losses to the comvided at the clinic. Methodology: For this study, we used
in home visits occurred during the fourth stage of expeWe cut out the four questions on the form, according to
health care unit. As the process of dispensing drugs only
that the presence of the pharmacist in the unit improve
in some way, the services offered. Conclusions: Thus, it
activity to psycholeptics drugs, without compromising the
motor coordination of animals in the Rota-rod test, deKeywords: HPA-05, derived imidazolidine, central
nervous system
CPE-143
Evaluation of antimicrobial and wound healing activities of the extract of Hymenaea stigonocarpa Mart.
ex Hayne
Lima, A F 1; Santos, F H P 1; Linhares, L A 2; Dimech,
G S 2; Chaves, C 2; Nascimento, M S2; Maia, M. B. S. 2;
Ximenes, E. A.2
1. Faculdade Integrada Tiradentes, Brazil, 2. Universidade Federal
de Pernambuco, Brazil
Introduction: The species Hymenaea stigonocarpa Mart.
ex Hayne, Leguminosae-Caesalpinioideae commonly
macology of the genus Hymenaea several researchers
The aim of present study was to evaluate the in vivo an-
primary health attention and pharmaceutical care services
28 strains of Staphylococcus aureus with phenotypes of
Keywords: Pharmacist; Primary Health Attention ;
Users.
PE-1434
Preliminary Psychopharmacological Study Of Imidazolidine Derived Hpa-05
used the wound mode in rats. Wounds were infected with
a Staphylococcus aureus Methicillin resistant strain and
treated, once a day, with the H. stigonocarpa hydroalco-
D.V.¹; Paulo, L.L.1; Souza, S.A2; Almeida, R.N.1; Morais,
L.C.S.L.1
1. Laboratório de Tecnologia Farmacêutica, Universidade Federal da
Paraíba, PB, Brasil.
2. Departamento de Química, Universidade Federal da Paraíba,
João Pessoa-PB, Brasil.
Backgroung: HPA-05 is an imidazolidine derivative, structurally similar to others of the same class, whose activities in the central nervous system was reported in previous
of animals treated with the H. stigonocarpa gel healed at
the end of the experiment. Healing rate of treated with
the H. stigonocarpa gel was higher than controls and this
of HPA-05 in animal models, using rodents. Methodology:
also tissue formation in more advanced stage of maturation than controls. Conclusion: More studies on the active
ment of a new drug.
Keywords: Hymenaea stigonocarpa, S. aureus, healing.
CPE-1438
Effect of a low-protein diet during pregnancy on pro-
received distilled water and tween 80, the experimental
ment, the animals were placed individually in the center of
eters. In Rota-rod test, the animals were treated and after
30, 60 and 120 minutes of administration were placed in
-
34
Xavier,T.F. 1, Silva, K.O.G. 2, Almeida, L.C.A.2, Canon,
F3, Manhães-de-Castro,R.4,Toscano, A.E.5
1. Centro de Ciências Biológicas,UFPE,Recife/PE
2. Departamento de Fisioterapia,UFPE,Recife/PE
3. Université de Technologie de Compiègne/França
4. Departamento de Nutrição,UFPE,Recife/PE
5. Centro Acadêmico de Vitória/UFPE, Vitória de Santo Antão/PE
Background: The protein-calorie malnutrition during the
critical period of development can alter the function of
all systems in the long run. In the muscular system, peri-
2011 5 (2)
(DBR). Methodology: Male Wistar rats were divided into
-
Keywords: Nanuza plicata, cytotoxicity, antitumor.
CPE-1440
Cytotoxic Activity of the Compound A398 In Tumor
and Non-tumor Cell Lines
Silveira, A. L.; Araújo, D.A.M.
Laboratório de Tecnologia Farmacêutica, Universidade Federal da
Paraíba, Brasil
Background: The Podophyllotoxin is a lignan found in
for many years for its potent cytotoxic activity. Due to
The images of muscle cells were recorded using computer
image analysis system Leica QWin. The counting of muswas measured and compared using the Student t-test (Sig-
portance as a chemical model in the development of new
fects of A398 (a new synthetic derivative of podophylof Industrial Property, INPI) in human colon tumor cells
determination if this agent differentially could affect cytotoxic activities in hepatic tumor cell lines (HepG2) was
also investigated. Methodology: We initially compared the
These changes can affect muscle function in the short and
long term.
CPE-1439
Evaluation of the cytotoxic effects of ethanol extract
from leaves of Nanuza plicata
Mangueira, V.M.1; Viana, W.P.1; Xavier, A.L.1; Pita,
V. S.1
1. Laboratório de Tecnologia Farmacêutica, Universidade Federal
da Paraíba, Brazil
loziaceae. It is a species rarely reported in the literature
tumor and toxicological activity in vitro of ethanol extract
of Nanuza plicata (NPE) in K562 tumor cells and mice
erythrocytes, respectively. Methodology: To in vitro antitu-
lines over a concentration range of 5 – 500µM using two
tests, MTT reduction and neutral red assays. In HepG2
ence of dexamethasone (CYP3A inducer), ketoconazole
tion). After 24 and 72 hours of treatment we do the cell
spectively, with 24h of treatment. This compound showed
of A398 in HepG2 cells. Pretreatment with dexamethasone decreased the cytotoxicity of A398 (IC50>250µM).
Conclusions: A398 was more cytotoxicity in HT29 than in
FGH and HepG2 cells. In addition, hepatocyte protection
the CYP3A isoforms and reactions of glucuronidation in
Keywords: Cytotoxicity, cancer, metabolism
for 72 h. In 96-well plate with cells under 72 h of treatsupernatant was carefully removed and added 100 µL of
read at 595 nm. To in vitro toxicological activity Swiss mice
-
pernatants was read at 540 nm to determine the concenNPE showed an in vitro antitumor activity with IC50 value
that NPE has in vitro antitumor activity and low toxicity
against erythrocytes, cells commonly affected in the treatments with anticancer agents.
CPE-1441
Involvement Of Potassium And Calcium Channels In
Tocolytic Effect Of Galetin 3,6-Dimethyl Ether On
Rat Uterus
G.A.1; Travassos, R.A.1; Souza, I.L.L.1; Queiroga,F.R.1;
Lira, D.P.1; Santos, B.V.O.2; Silva, B.A. 2
1 Laboratório de Tecnologia Farmacêutica,
2 Departamento de Ciências Farmacêuticas, UFPB, João Pessoa/
PB.
ceae) is popularly known in northeast Brazil as “juremaspasmolytic effect on rat aorta and guinea-pig trachea
through modulation of K+ channels that, indirectly, can
ation. FGAL showed spasmolytic activity on rat uterus
tives: To investigate the mechanism of tocolytic action of
35
Experimental Pathology and Health Sciences
CO2 mixture and resting tension of 1 g. Isometric and
isotonic contractions were registered. All the experimental
FGAL relaxed the rat uterus pre-contracted with 60 mM
± 0.1, respectively). We decided to investigate if FGAL
J.I.V.1; Filho, G.G.A. 1; Alves, H.S. 2; Chaves, M.C.O. 1;
Pessôa, H.L.F. 3
1. Universidade Federal da Paraíba, Laboratório de Tecnologia
Farmacêutica Prof. Delby Fernandes de Medeiros, João Pessoa-PBBrazil, Cx. Postal 5009.
2. Universidade Federal de Campina Grande, CEP 58540-000,
Sumé-PB, Brazil.
3. Universidade Federal da Paraíba, Laboratório de Bioquímica,
Genética e Radiologia, Departamento de Biologia Molécular, João
Pessoa-PB-Brazil.
cumulative curves of CaCl2 in the presence of FGAL (106 - 10-5 M), was shifted to the right with a decrease maxilites, aiming to highlight the presence of new compounds
pation of channels of K+ in this effect. Conclusions: The
relaxant effect of FGAL in rat uterus involves positive
and hemolytic activities of the ethyl acetate fraction and
Methodology: The plant material of Piper montealegreanum was collected in the municipality of Belém, State
Keywords: Galetin, spasmolytic, uterus
CPE-1442
Evaluation Of The Toxicity Of Pradosia Huberi Hydroalcoholic Extract On The Hypophysis-Gonadal
Axis Of Pregnant Female Rats
Rocha, A.O.B1; Souza, L.Q.S.1; Sousa, T. M1; Figueiredo,
R. R.1; Potiguara, R. K. S1.; Pita, J. C. R1; Santos, E.C.S.1;
Medeiros, A.A.N.2;Diniz, M.F.F.M.3; Sá, R.C.S.4
1. Program of Postgraduation in Natural and Syntactic Bioactive
Products, Center of Health Sciences – LTF/UFPB, Brazil
2. Institute of Technological Research of Amapá - IEPA, Brazil
3. Department of Pharmaceutical Sciences, Center of Health Sciences - UFPB, Brazil
4. Department of Physiology and Pathology, Center of Health Sciences - UFPB, Brazil
dicinal plant commonly found in the Amazonian forest
and popularly used to treat gastritis and gastric ulcer. The
jectives: In this study, the toxicological effect of the hywas investigated on the organs of the reproductive axis
(hypophysis, ovaries and uterus) of pregnant female rats.
(LH-20) column chromatography and their structures
13C NMR-500 and 125 MHz, respectively, IR and ESIfrom the genera Bacillus, Escherichia, Pseudomonas and
-
cytotoxic effect. Results: The ethyl acetate fraction showed
resistant to ampicillin at lower concentrations showing no
cytotoxic effect against human erythrocytes (A, B and O).
ampicilin and were not cytotoxic as well. Conclusions: The
-
four groups (containing 15 or 16 animals each), in which
Keywords: Piper montealegreanum, antibacterial activity, cytotoxicity.
of gestation (preimplantation phase). The animals were
tion. After laparatomy, the following organs were removed
ovaries and uterus. Results: The relative weight of the hy-
CPE-1444
Low frequency of intestinal parasites in inhabitants
of Sousa city – Paraíba – Brazil
Souza, L.Q. 1; Figueiredo, R. R.1; Monteiro, J.C. 1; Pereira,
J.C. 1; Leite, F.V.P1; Pequeno, I.F.P. 1; Lima, C.M.B.L. 1;
Cavalcanti, M.G.S. 1; Freitas, F.I.S. 1.
1. Department of Pharmaceutical Sciences, Center of Health Sciences - UFPB, Brazil
0,001) on the 8th day on gestation. The other parameters
hypophysis, which could interfere with the normal functioning of the hypophysis-gonadal axis.
Keywords: Pradosia huberi; reproductive toxicity;
hypophysis-gonadal axis.
CPE-1443
Antibacterial and hemolytic activities from Piper
Montealegreanum Yuncker (Piperaceae)
Pinto, D.S.1; Duarte, F.M.1; Delmondes, M.N.1; Costa
36
eradication of these parasites requires improvement of
socio economic, sanitation and health education. Aim: Investigate the frequency of intestinal parasites of Sousa
-
From 3921 tests performed, only 420 were positive for at
-
2011 5 (2)
thes. The most frequent parasite was Endolimax nana , fol-
quiring toxicity studies. Croton grewioides species is not
reported in the literature with regard to its pharmacologi-
The compiled data suggest that the studied community is
in a context that favors the maintenance and spread of
acute toxicity of essential oil from leaves of C. grewioides
(O.E.C.) in mice. Methodology: For the acute study, the
O.E.C. was administered in mice (i.p.) at doses of 250, 375
sample, along with the use of a single diagnostic technique,
greatly reduces the chances of detecting the parasites.
Keys-word: intestinal parasites, low frequency, Sousa
PE-1445a
In vivo antitumor activity and toxicity of essential oil
from leaves of Lippia microphylla Cham.
Batista, T.M.1; Sousa, T.K.G.1; Viana, W. P.1; Pessoa,
D.R.1; Xavier, A.L.1; Pita, J.C.L.R.1; Silva, T.G.2; Tavares,
J.F.1; Silva, M.S.1; Castello-Branco, M.V.S.1
1. Laboratório de Tecnologia Farmacêutica, Universidade Federal
da Paraíba, Brazil
2. Departamento de Antibióticos, Universidade Federal de Pernambuco, Brazil
Background: Many anticancer drugs currently used in
But natural products, as anticancer agents, can also cause
damage to the organism, requiring toxicity studies. Lippia
the in vivo antitumor activity and toxicity of the essential
oil from leaves of Lippia microphylla (OEL). Methodology: Eight-day-old sarcoma 180 ascites tumor cells (25 x
7 days. To the negative control group was administered
was evaluated the food and water consumption, as well the
matological analysis, and then all animals were euthanized.
Liver, kidneys, spleen, thymus and tumor were excised and
weighed. Results: The in vivo antitumor study demonstratanalysis showed moderate gastrointestinal and hematological toxicity. Alterations in liver function was also evidenced
widely anticancer drugs used in clinical medicine. Conclu-
and mortality were determined for up to 14 days. Indexes
of organs (liver, heart, kidneys, spleen and thymus) were
progressively with increasing doses. Some adverse effects,
such as sedation, anesthesia, decreased response to touch,
administration and were more pronounced at higher doses
in the indexes of heart, liver, kidneys, spleen and thymus
mals. Conclusion: O.E.C. showed moderate acute toxicity
when administered i.p. to mice. The LD50 value is used
conducted.
Keywords: Croton grewioides; acute toxicity; essential oil.
CPE-1448a
Evaluation of the antitumor activity and toxicity of
essential oil from leaves of Rollinia leptopetala RE
Fries
Mangueira, V.M.1; Brito, M.T.1; Batista, T.M.1; Moreira,
M.M.B.1; Sousa, T.K.G.1; Moura, A.P.G.1; Lunguinho,
D.M.1; Tavares, J.F.1; Diniz, M.F.F.M.1; Castello Branco,
M.V.S.1
1. Laboratório de Tecnologia Farmacêutica, Universidade Federal
da Paraíba
Background: Rollinia leptopetala R.E. Fries (Annonaceae),
ate the in vivo antitumor activity and toxicity of essential
oil from leaves of Rollinia leptopetala (E.O.R.). Methodology: An acute toxicity test (LD50) was performed to determine the doses of in vivo antitumor test. To in vivo antitumor activity, eight-day-old sarcoma 180 ascites tumor cells
vivo with moderate toxicity, which is not a limiting factor
Keywords: Lippia microphylla; antitumor activity;
toxicity.
CPE-1446
Acute toxicity of essential oil from leaves of Croton
grewioides Baill. (Euphorbiaceae) in mice
Sousa, T.K.G.1; Batista, T.M.1; Moreira, M.M.B.1;
soa, D.R.1; Lunguinho, D.M.1; Tavares, J.F.1; CastelloBranco, M. V.S.1
1. Laboratório de Tecnologia Farmacêutica, Universidade Federal
da Paraíba, Brasil
Background: Medicinal plants represent the largest source
ral products can also cause damage to the organism, re-
were euthanized and tumors and organs were excised and
and food and water consumption. Results: The LD50
tively. In O.E.R. treated groups, the toxicological analysis
increase in AST level and decrease in MHC and MCV with
37
Experimental Pathology and Health Sciences
increase of neutrophils, when compared to the control
group. The decrease in consumption of water and food
tumoral. However its effects on the immune system are
powder of Pc seeds (Ps) and its main active principles caffeine (Caf) and catechin (Cat), in the cellular immune re-
activity in vivo and moderate toxicity in experimental models evaluated.
Keywords: Rollinia leptopetala; antitumor activity;
toxicity.
CPE-1449
Pharmacological properties of Artichoke (Cynara
scolymus) extracts using thin-layer chromatography
bioautography assay (TLC)
Mattos, M.I.S1; Hansen, D2; Haraguchi, M2; Sartori,
1. Departamento de Patologia Experimental e Comparada, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São
Paulo – USP – Brazil
2. Centro de P&D de Sanidade Animal, Instituto Biológico – SP
- Brazil
3. Núcleo de Fisiologia e Bioquímica de plantas, Instituto de Botânica – SP - Brazil
methods, is an effective and inexpensive technique for the
study of plant extracts. Artichoke extracts are used for the
documented in a wide range of experimental and clinical
the different extracts and fraction from leaves of the aresterase activities using TLC. Methodology: TLC of the
methanol extract and the hexane and dichloromethane
Nystatin, quercetin and physostigmine were used as standards for antifungal, antioxidant and anticholinesterase
cladosporioides to verify the presence of fungitoxic zones;
other two plates were sprayed with DPPH (2,2-diphenyl1-picryl hydrazyl) and acetylcholine enzyme (AChE) for
antioxidant and anticholinesterase tests. The activity of
romethane extract showed highest anticholinesterase activity and moderate antifungal activity. The hexane fraction
ity. Conclusions: These results demonstrate that artichoke
extracts have a marked antioxidant, antifungal and anticholinesterase activities and TLC is an easy and fast assay for
these tests. Further research is needed for the safe use of
these extracts.
Keywords: Artichoke, Thin-layer chromatography.
CPE-1449a
Guarana, due to catechin, decreases the delayed-type
hypersensitivity (DTH) response
Caniceiro, B.D.1; Latorre, A.O.1; Carvalho, L.R.2; Naka1. School of Veterinary Medicine and Animal Science - University
of Sao Paulo, Sao Paulo, Brazil
2. Botany Institute of Sao Paulo, Sao Paulo, Brazil
Introduction: Seeking for immunomodulatory compounds
with therapeutic potential is constant due to the huge vaimmunological responses. In this context, it is known
that Paullinia cupana (Pc), popularly called guarana, is a
38
G (Ps), CF (Caf), CT (Cat) and FT (Caf + Cat). On Day
taneous (sc) sensitization with 50 µl of 1x108 sheep red
adjuvant solution). After 7 days, the diameter of the left
hind footpad was measured (0 hour). The mice were then
PBS only) into the left hind footpad. The increase in the
footpad diameter was measured 24, 48 and 72 hours later.
The swelling was calculated as the difference in diameter at
0 vs. 24, 48 or 72 hours and expressed in millimeters. Rethe CT group after 48 hours and in the mice from G, CT
and FT groups after 72 hours. Conclusion: These results
Keywords: guaraná; catechin; DTH.
CPE-1450
Importance Of Infection By T- Lymphotropic Virus
- Virus Type I (Htlv-I) In Connection Vertical Transmission
Machado, W.G.S.1; Santos, A.H.1; Pereira, B.D.S.1; Silva,
S.R.1; Cardoso, H.M1; Gomes, A.M.P.1,2; Nunes, M. K.
M.M.S.,4.
1. Santa Emilia de Rodat Faculty, student of Biomedicine, Brazil
2. Federal University of Paraiba, Brazil.
3. Oral Biology Laboratory, Health Science Center, Federal University of Paraiba, Brazil
4. Biological Science Center, State University of Paraiba, Brazil
Introduction - The human T-lymphotropic virus I (HTLV-
adults (ATL). He is also related to other non-malignant
-
of this virus in pregnant women, specifying ways to diagnose and valuing the use of primary prevention measures
to control the spread of the virus, especially for prophylaxis of vertical transmission. Methodology - This study
The HTLV-I is a retrovirus with oncogenic potential that
viduals, usually 20 to 30 years after infection. HTLV-I is
not easily transmitted, as the cell-cell contact is necessary.
In Brazil it is mandatory to carry out the tests to track the
2011 5 (2)
the prophylactic measures for vertical transmission are efKeywords: lymphotropic virus Virus type I; Vertical
Transmission; leukemia / lymphoma.
CPE-1452
study. We will analyze Giemsa and Papanicolaou-stained
slides of patients diagnosed positive for malignant cells
records of these patients, who underwent examinations
and History of Breast Cancer
Santos, G.T.; Zavalhia, L.; Camillo, N.D.; Brackmann, R.;
Prolla, J.C.; Bica, C.
1 – Post Graduation in Pathology - Universidade Federal de Ciências da Saúde de Porto Alegre, Brasil.
2 – Graduation in Medicine - Universidade Federal de Ciências da
Saúde de Porto Alegre , Brasil.
3 – Laboratory of Pathology of Complexo Hospitalar Santa Casa
de Porto Alegre/RS, Brasil.
This is a historical cohort study, in which we investigated
tistical analysis was performed using Stata. Version 9.0.,
Kaplan-Meier method to analyze survival. Results: We analyzed 145 patients with suspected malignant pleural effu-
of malignant pleural effusion and death. In patients with
we compared its expression in relation to progression to
death, and we noted that there was a higher occurrence of
death in the groups that did not express ER and PR and
related to the time of appearance of pleural effusion and
the pattern of survival. Results and Conclusion: Among
months. Our preliminary data indicate that, among the 27
mean survival was 3.1 months. Among the 27 slides anavival in this case was 3.5 months. Screening and analysis
of the slides of these patients with malignant cells are in
progress.
Keywords: breast cancer, pleural effusion, cell morphology.
CPE-1453
Evaluation Of The Expression Of C-Kit (Cd117) In
Ependymomas And Oligodendrogliomas Childhood
And Adults
Zavalhia, L.S.1; Santos, G.T.1; Romitti, M.2; Meurer, R.T.3;
1 Federal University of Health Sciences of Porto Alegre (UFCSPA), Brasil
2 Federal University of Rio Grande do Sul of the Rio Grande do
Sul (UFRGS), Brasil
3 Laboratory Research Pathology of Federal University of Health
Sciences of Porto Alegre (UFCSPA), Brasil
4 Department of Pediatrics of the Irmandade Santa Casa de Misericórdia of Porto Alegre (ISCMPA), Brasil
5 Department of Clinical Medicine of the Irmandade Santa Casa
de Misericórdia of Porto Alegre (ISCMPA), Brasil
Introduction: Oligodendroglial and ependymal tumors are
a rare group of neoplasms of the central nervous system
with multiple clinical manifestations, affecting children
and young people and adults, respectively. Currently, stud(CD117) can target the treatment to the use of selec-
with expression of this tumor marker. Conclusions: This
evaluate the expression of c-kit (CD117) in ependymomas and oligodendrogliomas and to estimate its usefulness
Keywords: breast cancer, pleural effusion, survival.
Methods: Specimens of ependymomas and oligodendroCPE-1452a
Effusion in Patients with History of Breast Cancer
1.UFSCPA – Graduation – Medicine;
2 UFCSPA – Post Graduation – Pathology;
3.Complexo Hospitalar Santa Casa - Porto Alegre/RS – Laboratory of Pathology.
Introduction: Breast cancer has the highest rate of cancer
mortality among women. Pleural effusion frequently appears during the course of the disease, and the emergence
immunoreactivity using anti-c-kit (CD117, DAKO, dilution 1:50). Were used as positive control gastrointestinal
stromal tumors. Results: The sample consisted of 34 ependymomas e 21 oligodendrogliomas. The median age at
diagnosis was 6.5 (1-65) years for ependimomas and was
38.7 years ± 16:52. for oligodendrogliomas. The analysis
immunohistochemistry demonstrated protein expression
for CD117. However, in oligodendrogliomas was showed
spread from the primary tumor, which reduces the life ex39
Experimental Pathology and Health Sciences
tial proportion of ependymomas and oligodendrogliomas
Keywords: Ependymomas; oligodendrogliomas; ckit.
CPE-1454
Anti-spasmolytic effect of essential oil of Lippia sidoides on isolated guinea pig ileum.
Arruda, I.T.S.1 ; Medeiros, M. A. M. B.1 ; Nascimento, R.
Silva, B.A.2 ; Souza, E.P.1
1. Laboratório de Bioquímica Molecular- DBM/UFPB, Brasil.
2. Laboratório de Tecnologia Farmacêutica – LTF/UFPB, Brasil.
3. Universidade Federal do Ceará – UFC, Brasil.
Introduction: Thymol, the major constituent of essential
oil of Lippia sidoides (EOLs) folkly known as “rosemary
pepper” has anti-spasmolytic effect in some smooth musEOLs on guinea pig ileum smooth muscle. Materials and
tive doses of EOLs dissolved in DMSO in concentrations
strongly interlinked. It was found that regions where there
is increase in mitosis predisposes the formation of atypical
the typical areas.
Keywords: glioblastoma, angiogenesis, mitoses.
CPE-1456
Sexual Orientation,Prevention of STDs, and Contraception Among Adolescents: First Observations on a
Public School in João Pessoa-PB
Rodrigues, M. L.¹ ; Leal, E.B.N. ¹ ; Martins, L.L ¹; Estevam,
1. Molecular Biology laboratory, College “Santa Emilia de Rodat”,
Brazil
2. Master of Bioactive Natural and Synthetic Products -UFPB,
Brazil
3. Graduating Student of Biomedicine “Santa Emilia de Rodat”,
Brazil
4. College of Biomedicine “Santa Emilia de Rodat”, Brazil
5. Coordinator of “Bemfam” São Luis-MA, Brazil
to start sexual life earlier, which ultimately increases the
chances of so-called “sexual accidents.” It is worthy to
-
-
fect to the control group. Conclusion: EOLs reduced contraction of guinea pig ileum smooth muscle treated with
ther studies on the relaxing pharmacological mechanism.
Keywords: L. sidoides; contractility; ileum.
themselves from sexually transmitted diseases (STDs)
and unwanted pregnancies. Methodology: We performed
video lectures and a data collection through a questionnaire containing questions such as: (When was the 1st
intercourse, what they thought of contraception such as
condoms, pills, injections). Applied to 20 students in 9th
grade of the Municipal School “Violeta Formiga” located
in Mandacaru, João Pessoa- PB. Results: The 20 students
CPE-1455
of glioblastomas
Pinto, A. P. S.1, Lima, A. F. L.1, Duarte, S. R.1, Mello, R. J.
V.2, Montenegro, L. T.2, Pontes-Filho, N. T.2
1. Faculdade Integrada Tiradentes, Brazil
2. Universidade Federal de Pernambuco, Brazil
as the main prerequisite for tumor growth and metastathat secrete pro-angiogenic factors altering the structure
of the vessel, making it similar to a renal glomerulus. The
study of indicators of anaplasia (mitosis, hypercellularity,
necrosis in palisading) in region surrounding typical and
importance of the normal or changed vascular structure
and after morphological analysis selected cases that presented atypical vasculature. In each case we analyzed the
mitosis parameter in the typical vascular areas to compare
40
ceptive method against STDs and unwanted pregnancies,
active.Conclusion: The data in this paper on Sexual Orientation suggest that students recognize the importance of
contraception and prevention against STDs and unwanted
pregnancies, since all students that are sexually active do
sexually active agree with sexual protection.
Keywords: Sexual Orientation; STD (Sexually Transmitted Diseases); Pregnancy.
CPE-1456a
Biochemical parameters in animals acutely treated
with Zizyphus joazeiro Mart. leaves
Estevam¹ E. C.; Vilarim3, R. M.; Silva4, A. R. M.; Souza4, J.
M. F. F. M.
¹Laboratório de Tecnologia Farmacêutica – Universidade Federal da
Paraíba/ UFPB, Brazil.
²Departamento de Biofísica e Radiobiologia – Universidade Federal
de Pernambuco/ UFPE, Brazil.
3Departamento de Biologia Molecular – Universidade Federal da
Paraíba/ UFPB, Brazil.
2011 5 (2)
4 Faculdade Santa Emília de Rodat/ FASER, Paraíba, Brazil.
Background: Zizyphus joazeiro Mart. popularly known
for skin and stomach diseases, coughing and as a hair
study was evaluate the pre-clinical toxicity of Zizyphus
agents, suggesting that the Sp-EtOHE displays gastroproactivity.
-
coholic extract of the leaves of Z. joazeiro (EHAB). One
of serum glucose, urea, creatinine, uric acid, cholesterol,
triglycerides, ALT and AST. The results are expressed as
ed male group presented a decrease in levels of uric acid
alteration was detected in the females treated with extract.
high dose of EHAB administered, indicating altered liver
vonoids.
CPE-1459
Clinical manifestations of the disease from the mitochondrial respiratory chain
Leite, G.G.F.1,3, Araújo, V.S.1, Neves, J.W.J. S. 2,3; De Sousa, T.A.¹, Santos, E.P.¹ Estevam E. , 1,3
1. Faculdade Santa Emília de Rodat, Brazil
2. Laboratório de Percepção, Neurociências e Comportamento –
LPneC/UFPB, Brazil
3. Universidade Federal da Paraíba, Brazil
Introduction: The diseases from mitochondrial respiratory
eases which has had a growing recognition; these diseases
have constituted a group of diseases of heterogeneous
gation to show the main clinical manifestations of MRCD,
could have increased the renal excretion of urate, causing a decrease in the serum uric acid. Conclusions: The
made in a cautious way.
Keywords: Zizyphus joazeiro leaves, toxicity.
CPE-1457a
Gastropotective Effect Of Sidastrum Paniculatum
Fryxell (Malvaceae)
Leite, T.J.A.1; Sales, I.R.P.1; Montenegro, C.A.1; Lima,
G.R.M.1; Jesus, N. Z. T.1; Vanderlei, M.F.1; Batista, L.M.1
1. Laboratório de Tecnologia Farmacêutica – Universidade Federal
da Paraíba, Brasil
Background: Sidastrum paniculatum Fryxell popularly
known as “malva-roxa” and “malvavisco”, is a tree with
which met our goals. Discussion: The MRCD are multi
logical systems and the fact that it is produced mainly in
the mitochondria. Being the main clinical manifestations
the neurological symptoms, such as a delay in the psychomotor development, seizures, encephalopathy, myoclonus,
known are the non neurological manifestations, it may
cause changes in the ocular system as ophthalmoplegia and
pigmentary retinopathy, the cardiovascular system shows
phic cardiomyopathy, in the endocrine system is frequent
liver disease and weight loss. In most cases the diagnosis
gastroprotective effect of the crude ethanolic extract (SpMRC. The treatment is guided in order to minimize the
Male Wistar rats (Ratus norvegicus) (180-250 g) or Swiss
mice (Mus musculus) (25-35 g) were treated with Sp-EtO-
many clinical manifestations of the MRCD due universal
induced ulcer model in rats, Sp-EtOHE (125, 250 and 500
in mice, Sp- EtOHE also showed gastroprotective effect
diagnosis.
Keywords: mitochondrial, disease, multi systemic.
CPE-1459a
Perspectives of minor beta thalassemia from a case
report
De Sousa, T.A.1; Leite, G.G.F.1,3; Araújo, V.S.1; Nunes,
I.A.S.1; Dias, G.A.1; Santos, G.L.2,
1. Faculdade Santa Emília de Rodat, Brazil
41
Experimental Pathology and Health Sciences
2. Universidade Federal de Campina Grande, Brazil
3. Universidade Federal da Paraíba, Brazil
common the presence of pathogens in these foods, such
Keywords: Parasites, lettuce, sedimentation.
minor (heterozygous) is a genetic alteration associated with
-
on a clinical case. Methodology: This case report concerns
tient, female, had a microcytic hypochromic anemia, with
-
CPE-1460
In vivo studies of Poliamidoamine (PAMAM)
99mTc(CO)3-dendrimer-FITC in murine breast tumor model as a nano-carrier of anti-tumor drugs
Oddone, N.1,2; Tassano, M.2; Fernández, M.2; Kramer,
Benech, J.C.1
1- Instituto de Investigaciones Biológicas- Ministerio de Educación y
Cultura, Uruguay; 2- Centro de Investigaciones Nucleares- Universidad de la República, Uruguay; 3- Instituto de Higiene- Universidad
de la República, Uruguay
Background: Poliamidoamine (PAMAM) dendrimers
implanted in syngenic mice and lung metastases developed
in mice with primary tumor surgically excised. Methodol-
make appropriate tests for diagnosis. If the father is hetwere done. Activity was measured at 1 and 3 h and the
-
lyzing cryostat sections from liver, lung and lung metasta-
tary
CPE-1459b
Search helminths and protozoa in samples from lettuce (Lactuca sativa) commercialized in the city from
João Pessoa - PB
Leite, G.G.F.1; De Sousa, T.A.1; Araújo, V.S.1; Souza, J.P.1;
Gomes, A.M.P1; Cardoso, H.M.1; Carvalho, M.M.S.G2
1. Faculdade Santa Emília de Rodat, Brazil
2.Universidade Estadual da Paraíba- UFPB, Brazil
Background: The survey of helminths and protozoa in let-
parasites and other pathogenic structures in food as a matter harmful to human health. And so, food marketed to
of helminths and protozoa in samples lettuce (Lactuca sativa) commercialized in markets, greengrocers and street
Samples were collected randomly in fairs, markets and
Each sample was washed with distilled water in a stainless steel container, after which the water transferred to
a glass of sedimentation. The sediment was analyzed in
triplicate after 24 hours, with Lugol in optical microscope.
-
We conclude that it is very important proper washing of
42
99mTc(CO)3-dendrimer-FITC in mammary tumor mice
-
0.05, 0.3 ± 0.2. Confocal microscopy analysis of liver and
h of 99mTc(CO)3-dendrimer-FITC injection, showed cytumor surgically excised. Conclusions: These results indicate that Poliamidoamine PAMAM dendrimers, are good
mor and its metastases.
Keywords: Poliamidoamine (PAMAM) 99mTc(CO)3dendrimer-FITC; breast tumor mice; lung metastases.
CPE-1461
Kidney structural changes in mice treated with DioAnalysis
Soares, P.H.S.1; Silva, L.L.S.1; Lima, C.E.Q.2; Bittencourt,
A.M.3; Silva, M.G.S.1; Silva, J.F.4; Silveira, A.B.2; Almeida,
E.R.5; Silva, C.V.N.S.6; Mota, D.L.1
1. Departamento de Histologia e Embriologia – Universidade Federal de Pernambuco, Brazil
2. Departamento de Ciências Farmacêuticas – Universidade Federal
de Pernambuco, Brazil
3. Departamento de Anatomia – Universidade Federal de Pernambuco, Brazil
2011 5 (2)
4. Departamento de Ciências Morfológicas – Universidade de Pernambuco, Brazil
5. Departamento de Antibióticos – Universidade Federal de Pernambuco, Brazil
6. Laboratório de Imunopatologia Keizo-Asami – LIKA/Universidade Federal de Pernambuco, Brazil
Introduction: The use of plants in the called “folk medicine” is a characteristic of Brazilian cultural heritage.
Among the numerous plants popularly used Dioclea granis used in the treatment of renal and prostate diseases.
32 g were used, 10 animals for experimental group (ExG)
and 10 for control group (CG). Both groups were maintained in an animal house with average temperature of
for seven consecutive days. The extract dosage used for
-
fests in pregnancy and that interferes with the emptying
ing pregnancy, normally, shows constipation. The stools
and rectum causing discomfort and malaise. Conclusion:
One of the indicated strategy is to monitor food given,
apies, which during pregnancy can compromise the formation of the fetus.
Keywords: pregnant women; gastrointestinal tract;
constipation.
CPE-1463a
Epidemiological survey of viral hepatitis in patients
with HIV in Paraíba State
Benício, T.C.M¹; Menezes, R.C ¹ ; Souza, J. P.¹ ; Santos,
1.Faculdade Santa Emília de Rodat, Brazil
2.Universidade Federal da Paraíba, Brazil
procedures of ExG. Kidneys were excised, medially secB, C, D, and, F, G. Other causes include medicines, metatis has some destruction degree of the hepatic cells. In
-
-
was used. Results: A marked glomerular hypertrophy was
Virus (HIV) the development speed of hepatitis is faster,
while in ExG the value was of 450.44 µm2. Conclusions:
Results showed histological changes in renal corpuscles of
years. In this study was made an epidemiological survey of
injury to renal function.
-
Complex Clementino Fraga, in João Pessoa city, Brazil, for
etry
CPE-1462
Pathophysiologic changes in the gastrointestinal tract
cife
Farias, L.L. ; Aires, T.R.B. ; Silva, A.O.R. ; Souza, I.A. ;
Anjos, F.B.R. ; Ramos, R.C.
the patients had HIV. The highest incidence in the year
was in August with 58 cases and less in May, with 7 cases.
undergoes changes during pregnancy. At this stage the
gastrointestinal motility decreases, even with increased
of the hepatitis epidemiology, since in the last 20 years,
epidemiological and natural history studies of this disease
this study was to identify the incidence of gastrointestiin maternity of Recife. Methodology: This research was
transversal, trough semi-direct questionnaires applied in
Professor Bandeira Filho maternity, in the years of 2006
and 2007. The analyzed data were: family income, education level, and clinical symptoms. The women were iden-
-
diagnosis test.
CPE-1464
Action Of Wine On The Liver Of Rats Submitted To
DMH Administration
Jorge, M.1,3 ; Duarte, R.3,4; Costa, M.3,4 ; Costa, E.1,3 ;
1- Faculdade de Farmácia da Universidade de Coimbra, Portugal.
2 -Universidade Federal de Pernambuco, Brasil.
3 -Faculdade de Medicina da Universidade de Coimbra, Serviço de
Patologia Experimental, Portugal.
4- Escola Universitária Vasco da Gama, Portugal.
43
Experimental Pathology and Health Sciences
are still under investigation.
In this study we evaluate liver morphologic changes associated with red wine and dimetilhidrazine consumption
(DMH), a well known carcinogen.
-
to the surgical intervention, and no qualitative or quantitative differences among the groups. The thicknesses of
studied. Immunoexpression of PGP9.5 demonstrates in-
-
week and a complete necropsy was performed. The slides
ated for morphologic lesion detection.
In the group I there was not found any morphologic
changes.
In the group II it was found: sinusoidal congestion, fo-
and in fecal exoneration, thus restoring the evacuatory act
close to the functional standard; 3) produce denervation
exclusively at the site of their construction; 4) generate
adaptive intestinal response with the preservation of the
histological structures in the operated intestinal intervalve
valves; 6) transform the intestinal segment where they are
constructed into a storage segment. The technique is easily
performed and has important potential for clinical application.
Keywords: Biological valves, Myenteric plexus,
Colorectal surgery.
nuclei with increased nucleoli.
edema and sinusoid congestion. Nuclear and cytoplasmic
diffused changes, such as pyknosis, increased nuclei, macrovesicular and microvesicular steatosis, and cytoplasmic
According to these results we may conclude that red wine
CPE-1465
Morphology and immunohistochemistry of the myenteric plexus of valves constructed in the colon of
rats subjected to abdominoperineal amputation and
perineal colostomy1
Deoti, B.1*; Campos,C.1; Costa-Silva I.C.1, Hayck, J.2
Lázaro da Silva, A.3 ; Arantes, R.M.E1.
1.Departamento de Patologia Geral, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte/MG,
Brasil.
2. Physician, Resident in General Surgery at the Cristiano Machado
Hospital.
3. Professor Emeritus at the Federal University of Minas Gerais
(UFMG), Head of the General Surgery Service at the Cristiano
Machado Hospital, FUPEC / FHEMIG.
their sphincter structure and the colon pull-through via
perineal colostomy. Amputated group with surgical valve
(AGWV), 24 animals underwent three seromiotomy procedures on the pulled-through colon. Results: On the 40th
postoperative day the intestine was processed for light microscopy (HE), morphometric and immunohistochemical
analysis (PGP 9.5). The HE microscopy reveals microscopic linear interruption of muscle layers and chronic
interruption of innervation at valve sites. Areas adjacent
to the valves showed well-preserved structural aspect. Gomori trichrome stain showed deposits of collagen near
44
CPE-1465b
Experimental Trigeminal Neuralgia: Morphological
Alterations And Pain Development
Oliveira, A.P.1; Licio, C.S.A.2; Siqueira, J.M.3; Massenssini,
A.R.3; Moraes, M.F.D.3; Tafuri, W.L.4; Arantes, R.M.E. 4;
Almeida-Leite, C.M.1
1.Departamento de Morfologia/ICB/Universidade Federal de
Minas Gerais, Brasil,
2. Departamento de Ciências Biológicas, ICEB/Universidade Federal de Ouro Preto, Brasil,
3. Departamento de Fisiologia e Biofísica, ICB/Universidade Federal de Minas Gerais, Brasil,
4. Departamento de Patologia Geral, ICB/Universidade Federal de
Minas Gerais, Brasil.
injuries to the nervous system. Trigeminal neuralgia is a
neuropathic pain localized to small areas of the face. An
experimental rat model of trigeminal neuropathic pain is
neuropathic pain can develop from structural impairment
aim of this work was to study the morphological alterations that underlie pain development in the rat experimental model of trigeminal neuropathic pain. Methodology:
group) or a sham operation without nerve ligation (Sham
ated at postoperative days 3, 6, 9, 12 and 15. Trigeminal
nerves were dissected and processed for optic and electronic microscopy. Results: CCI group rats showed an
early hyporesponsiveness and a late hyperresponsiveness,
and lesioned axons, edema in nerve fascicles, and loss of
tissue organization. Demyelination and degenerated mypain states in humans and experimental models, were also
and detachment of myelin. Morphometric analysis showed
greater celularity in CCI group nerves, which can occur
as a result of glia proliferative response to nerve lesions.
vealed severe structural impairment of nerve fascicles and
2011 5 (2)
trigeminal neuropathic pain development.
KEYWORDS: neuropathic pain, trigeminal nerve,
demyelination.
-
APQ-00489-09)
CPE-1465c
Aspects Of Interaction In Vitro Between Microgliocytes And Leishmania Major
Souza, C.C.1, Costa-Silva, I.C.1., Campos, C.F.1 , Cangussú, S.D.2,; Almeida-Leite, C.M3, Vieira, L.Q.4, Arantes,
R.M.E1
1- Departamento de Patologia Geral, ICB - UFMG - Belo Horizonte/MG, Brasil.
2- Departamento de Ciências Biológicas, ICEB - UFOP - Ouro
Preto/MG, Brasil.
3- Departamento de Morfologia, ICB - UFMG - Belo Horizonte/
MG, Brasil.
4- Departamento de Bioquímica e Imunologia, ICB – UFMG,
Brasil.
Background: Microgliocytes (MG) are central nervous system cells that share origin and function of macrophages
odology: We investigated the in vitro interaction of MG of
rosy were selected, all of them were in treatment for less
than 30 days and presented at least one cutaneous lesion.
sensory analyzer was used. For morphological analysis,
-
Package for Social Sciences (SPSS) 15.0 software. Results:
The imunohistochemical staining showed decrease of in-
density was intense and homogeneous. Conclusions: The
skin lesions is an investigative and important tool to understand the mechanisms of disease that involve peripheral
-
culture for trials interaction of one hour (adhesion) and
48 h (infection) with L. Major. The participation of CR3
Financial Support: CNPq and FAPEMIG grants
anti-CR3. Results: MO shows higher rate of adhesion than
was higher. Addition of SD increased adhesion parameters
show different characteristic regarding the interaction with
tion of SD. Addition of SD and anti-CR3 showed the direct participation of CR3 in the interaction. However, as it
a participation of an alternative interaction and internalizaKeywords: Microgliocytes, central nervous system,
macrophages.
CPE-1465d
Correlation Between Density Of Nerve Fibers In
Skin Biopsy And Pattern Of Termic Sensibility In Patient Lesions With Leprosy
Lyon, S.2, Villarroel, M.F.3, Arantes, R.M.E.1
1Departamento de Patologia Geral, UFMG, Belo Horizonte/
MG, Brasil
2Fundação Hospitalar do Estado de Minas Gerais, Belo Horizonte/MG, Brasil
3Departamento de Medicina Tropical, UFMG, Belo Horizonte/
MG, Brasil
CPE-1465e
lated To Variation In Nociceptive Thresholds Of
Mice Ear Leishmania Major-Induced Lesions
Cangussú S.D.1; Souza CC2; Campos C.F.2 Vieira L.Q3;
Afonso L.C.C1; Cunha FQ4; Arantes R.M.E. 2
1Departamento de Ciências Biológicas, ICEB, UFOP, Ouro Preto,
MG, Brasil
2Departamento de Patologia Geral, ICB, UFMG, Belo Horizonte,
MG, Brasil
3Departmento de Bioquímica e Imunologia, ICB, UFMG, Belo
Horizonte, MG, Brasil
4Faculdade de Medicina de Ribeirão Preto, USP, São Paulo, Brasil
healing lesions and Th1 response. Th1 and Th2 cells seel, we investigated variation in nociception along 12 weeks
(wk) post infection and its relationship with structure of
morphological analysis indicate variation in the aspects of
nociception in wk 2, related to increased levels of IL-6,
in ear. Exogenous IL-10 was demonstrated to prevent the
hypernociception in this infection model. In support to
The evolution of disease cause severe damage and perma-
endogenous IL-10 in hypernociception. Moreover, in this
45
Experimental Pathology and Health Sciences
(SRE) in the spleen and liver. The erythrocytes decrease
the survival time of these cells, through their removal from
-
-
the mechanisms of hyponociception may envolve downregulation of cytokines, preservation of nerve endings
structure, differences in neurotransmitter release or some
in patients with self immune diseases. The evolution is ex-
MIG grants
KEYWORDS: cytokines, nociception, L. major infection
taneously, after months or years of evolution. The incicommon in males, however, is often in females during the
CPE-1466
implants in Ehrlich tumor-bearing mice
Da Silva, G.R.1; Pereira, A.F.1; Costa, V.M.1; Santos,
M.C.M.1; Pinto, F.C.H.1
1. School of Pharmacy, Federal University of São João Del Rei,
Divinópolis, Brazil
AHAI remain unknown. Some hypotheses are: the depresalteration among the T cells facilitators and suppressors,
changes of the antigen from surface of the erythrocytes
release delivery systems of therapeutic agents incorporated
that, it is so important, that the professional from the
to provide the patient quality assistance.
mice was investigated. Methodology: Implants were preimplants without MTX were also produced. In vivo release
taneous tissue of mice. A total of 20 Swiss female mice,
two months old were divided into two groups of 10 anicells (0.05 mL) on the footpad. At 5 days post-inoculation,
the animals of the A and B groups received PCL implants
paw thickness, using a digital pachymeter. At 12 days post-
CPE-1468
Current understanding of the multi form glioblastoma (GBM) and new therapeutical potentials
Santos, A.H.1; Pereira, B.D.S.1; Silva, S.R.1; Cardoso,
H.M.1; Gomes, A.M.P.1,2; Nunes, M. K. S 1,2; Silva,
B.D.F.1; Machado, W.G.S.1; Santos, E.P.1; Souza, J. P1,2;
1. Santa Emilia de Rodat Faculty, student of Biomedicine, Brazil
2. Federal University of Paraiba, student of Technical Biodiagnostic, Brazil.
3. Biological Science Center, State University of Paraiba, Brazil
4. Oral Biology Laboratory, Health Science Center, Federal University of Paraiba, Brazil
astrocytic tumor with higher degree of malignancy, cor-
cutaneous tissue of mice. Animals of the B group showed
of the World Health Organization (WHO). It is a primary
tumor from the central nervous system more common in
drastic decrease of cellularity in the B group. Conclusion:
Related To Variation In Nociceptive Thresholds Of Mice
Ear Leishmania Major-Induced Lesions high capacity of
and polymorphic cells. Its growth is often in the fronKeywords: methotrexate-loaded poly(caprolactone)
implants; Ehrlich tumor; local drug delivery system.
CPE-1467a
Clinical and therapeutical aspects of the self immune
hemolytic anemia (AHAI)
Benício, T.C.M ¹ ; Santos, E.P ¹ ; Diniz, D.A.M¹ ; Souza,
vide a current understanding of GBM and discuss the
new future therapeutical target potentials. Methodology:
Through a systematic known literature review, as well as
a critical analysis and comparative of works, articles from
start to elucidate a multi form network of genetic changes
1.Faculdade Santa Emília de Rodat, Brazil
2. Universidade Federal da Paraíba, Brazil
The self immune hemolytic anemia is a disorder from idiopathic source or secondary to other self immune diseases
-
46
migration and invasion, opening the door to new discovershown that genetic loss, due changes in the genomic level,
erozygosis of the chromosome 10, the most frequent al-
2011 5 (2)
-
3.Santa Emilia de Rodat Faculty, Brazil
Introducion: Falciform anemia is a genetic disease of
(EGFR) in chromosome 7. Perhaps the greater mystery
in the comprehension of the oncogenomic of GBM is to
decide how and when these genetic changes and the signaling cascade interact. Conclusion: Clearly, many of these
There is a real hope for patients who suffer with this illness
in the future.
Keywords: multi form glioblastoma; astrocytic tumor.
CPE-1469b
Hereditary Hemochromatosis: “Rust in the body”
-
of the molecule changing its charge and electrophoretic
cidate the importance of genetic counseling in hereditary
occurrence of sickle cell anemia, for individuals previously
the gene, are assisted and know the risks of recurrence for
-
1. Santa Emilia de Rodat Faculty, student of Biomedicine, Brazil
2. Molecular Biology laboratory, Santa Emilia de Rodat Faculty,
Brazil
3. Master of Bioactive Natural Products and Synthetic, State University of Paraiba, Brazil
40 to 160 micrograms, there are those who accumulate in
large quantity than necessary, carriers with hereditary hemochromatosis (primary), an autosomal recessive disease
arm of chromosome 6, which secretes a protein trasmem-
tients with anemia or sickle cell trait. Results: According
to the Ministry of Health estimates that there are more
clinic complications and other relatively common. Overall
ity to infections. The little foot exam is used for detecgene therapies are limited. Conclusion: Genetic counseling
mochromatosis through a literature review at documents
less frequent S65C. Results: The mutation from most
clinically relevant is it G -> A at nucleotide 845, resulting
(C282Y) and decreasing the density of the protein HFE
dine to aspartic acid (H63D) compromises the interaction
position 65, due to the transversion of adenine nucleotide
A -> T, at position 193 of the HFE gene. Conclusion:
The mutations genetic screening allows the knowledge
and awareness of genetic risk of disease development, and
early diagnosis of individuals at an early stage. There is
sickle cell disease or trait, requiring implementation of a
program of early diagnosis and genetic orientation, psychological and social support for these individuals.
Keywords: genetic counseling, sickle cell anemia,
public health.
CPE-1482
Morphometric changes in hearts of rats under malnutrition and chronic exercise during the period of
lactation
Alves, R. M. R.¹; Guendler, J. A.¹; Pontes Filho, N. T.¹;
Melo Júnior, M. R.¹; Araújo Filho, J. L .S.¹ ; França, T. J. B.
1.Department of Pathology, Federal University of Pernambuco
(UFPE), Brazil
2.Department of Anatomy , Federal University of Pernambuco
(UFPE), Brazil
Background: Exercise and undernutrition can cause different effects in heart structure and function. However hearts
morphometric changes are not totally explained, mainly
in developmental period, when some damages induce the
ate the morphometric changes in heart of young malnour-
iron, where accumulates in the liver, pancreas and heart,
can lead to death from cirrhosis, hepatocellular carcinoma,
males Wistar rats in lactation period were divided in four
Keywords: Iron, Mutation, Hemochromatosis
exercise (NE); undernourished control (DC) and under-
CPE-1469c
The importance of genetic counseling in sickle cell
anemia
Leal, E. B. N.¹; Rodrigues, M. L.¹; Martins, L.L ¹ Estevam,
gressive program of daily sessions of free swimming carried through 8th to 23rd days of life. After, infusion was
1. Molecular Biology laboratory, Santa Emilia de Rodat Faculty,
Brazil
2. Master of Bioactive Natural Products and Synthetic, State University of Paraiba, Brazil
47
Experimental Pathology and Health Sciences
in the nourished than in malnourished, no difference rewas higher for NC and NE than for DC and DE groups.
Brazil
2-Departamento de Fisioterapia – CCS/UFPE, Brazil
3-Departamento de Patologia – CCS/UFPE, Brazil
Background: Exercise and undernutrition can cause differ-
higher than the NC group. There were no differences in
cleus were higher in nourished groups than malnourished
and in NE than NC. Conclusions: Protein undernutrition
associated with exercise during the lactation period did not
gly, exercise and undernutrition caused changes in heart
sults. We concluded that protein malnutrition leads to a
CPE-1482a
Analysis of neurons NADPH-diaphorase in the cerebral cortex of animals exposed to perinatal malnutrition and alcoholism
1. Institute of Integrative Medicine - Professor Fernando Figueira/
IMIP, Brazil,
2. Department of Pathology, Federal University of Pernambuco
(UFPE), Brazil
Background: The various effects of ethanol and malnutrition to the Central Nervous System such as microcephaly, mental retardation, motor dysfunction and cognitive
impairment are well understood and widely recognized.
However, the mechanisms that induce these changes still
reason, the present study evaluated, through morphometric parameters, the effects of malnutrition and ethanol over
length of the primary extensions of Neurons NADPHdiaphorase and the neuronal density in cortical layers of
zone of 40 day-old Wistar male rats, generated and sucktein) and alcohol administration (commercial aguardente
administered treatment (Nutrition (N), Nutrition Brandy
(NB), Nutrition Ethanol (NE), Malnutrition (M), Malnutrition Brandy (MB), and Malnutrition Ethanol (ME). The
-
morphometric changes are not totally explained, mainly
in developmental period, when some damages induce the
ogy: 42 males Wistar rats were tested during the lactation
period. The animals were exposed two different nutritional
with swimming: well-nourished control (NC); well-nour-
of daily sessions of free swimming carried through of 8th
to 23rd day of life and they swam during 15, 20, 25, 30 and
ment the somatic development parameters were evaluated:
corporal weight, latero-lateral axis of skull, length of tail
metrical analysis evaluated the cellular area and density of
the neurons of motor cortex somatic development. Conclusions: Our results indicate that the proposed swimming
parameters evaluated, as well as it did not interfere in the
of the neuronal cellular area.
Keywords: protein malnutrition, exercise and brain
CPE-1483
Plants of the genus Bauhinia inhibit the activity of
gelatinase B
R.I.M.A1.
1 Universidade Federal de São João del-Rei - Campus Centro-Oeste
Dona Lindu, Brazil.
Background: The matrix metalloproteinases (MMPs) are
endopeptidases with degrade components of the matrix
extracellular in physiologic e pathologic process. MMP-9
related to studies involving tumor progression and metastasis. Plants of the genus Bauhinia, known as Pata-de-vaca
tives: To assess the activity of crude extracts, hexane, leaf
C) of the genus Bauhinia, on the proteolytic activity of geneuronal density as well as the NADPH-diaphoreses neu-
of Bauhinia: 1A, 1B, 1C, 2A, 2B, 2C, 3A, 3B, 3C. Areas of
and densitometry was performed using Scion Image.
Phytochemical tests to detect the presence of secondary
during the perinatal development.
Keywords: ethanol, malnutrition, cerebral cortex
CPE-1482b
Morphometric changes in hearts of rats under malnutrition and chronic exercise during the period of
lactation
Guendler, J. A. 1; Alves, R. M. R.1; Melo Júnior, M. R. 1;
Araújo Filho, J. L .S. 1 ; França, T. J. B. M 1; Ferraz, K. M.
2; Pontes Filho, N. T. 1-3; Costa, M. L. C. R.3
1-Laboratório de Imunopatologia Keizo Asami – LIKA/UFPE,
48
statistical analysis, we found that all of Bauhinia extracts
C2, C3, B2, A2, B1, respectively. The phytochemical study
of extracts was positive for steroids, triterpenoids, couma-
2011 5 (2)
-
Keywords: Gelatinase B, Bauhinia, Zymogram.
CPE-1484
Evaluation of the immunomodulatory effect of
monoterpenes in experimental arthritis
Brasileiro-Silva, L.A.¹; Ramalho, T.R.¹; Moreira, M.M.B.2;
Castello-Branco, M.V.S.2; De Sousa, P.D3; Piuvezam,
M.R.1; Bezerra-Santos, C.R.1
1. Laboratory of Imunopharmacology, Federal University Paraíba,
Brazil
2. Laboratory of Toxicology, Federal University of Paraíba, Brazil
3. Department of Physiology, Federal University of Sergipe, Brazil
Background: Rheumatoid arthritis (RA) is a systemic au-
buco (UFPE), Brasil
2. Departamento de Bioquímica - UFPE, Brasil
3. Departamento de Física - UFPE, Brasil
4. Laboratório de Pesquisas em Virologia - Faculdade de Medicina
de São José do Rio Preto (FAMERP), Brasil
posites (NcAuPANi) associated to Cratylia mollis lectin
(CramoLL) for detection of dengue serotypes 1 and 2.
-
were used as counter electrode and reference electrode,
respectively. The electrochemical impedance spectrosare well known. Natural products therapies using mono-
for the characterization and interfacial monitoring at dif-
study was to analyze the potential immunomodulatory efmonoterpenes were tested for their toxicity using Artemia
-
owing to the adsorption of NcAuPANi–CramoLL, and
dengue serum. It demonstrated that CramoLL retained
opment the delayed-type hypersensitivity reaction (DTH)
edema was performed in different periods of time after
challenge procedures. Results: There was no toxic effect
After OVA-sensitization and -challenge, mice developed a
ed only with soil oil (vehicle). Oral treatment (p.o) with
3.78 ± 0.36, respectively) in comparison with non-treated
(3.73 ± 0.74; 4.02 ± 0.8, respectively) also did not alter paw
edema formation in comparison with non-treated mice
(3.88 ±0.62)). In a separate experiment, mice were treated
for 28 days after challenge and also did not reduce paw
edema formation. Similarly, dexamethasone (p.o.) also did
not reduce paw edema formation indicating that after inmation status unless used at long period of time. Concluthat these monoterpenes might present protective effects.
Keywords: Monoterpenes, arthritis, mice
recognize glycoproteins present in dengue serotypes 1 and
Keywords: biosensor; gold nanoparticles; lectins.
CPE-1487
Masseter evaluation in experimental intoxication with
Tioacetamide
Joseíde Félix1,2, Joana Costa3, Felipe Pereira4, Rodrigo
Farinha2, Maria Emilia Ramos5
1 Graduating in Dentistry- State University of Feira Santana
2 Experimental Pathology Service, Faculty of Medicine, University
of Coimbra
3 Chemistry Department, Faculty of Sciences and Technology, University of Coimbra
4 Faculty of Pharmacy, University of Coimbra
5 Department of Health, State University of Feira de Santana,
Feira de Santana, BA, Brazil
or a consequence of the symptoms linked to temporotoxic is a source of sulfur in the industry, used for organic
production, as well as in metallurgy and for pesticide synthesis. Tiocetamide, is an experimental inductor of hepatic
disease in the rat, producing lesions from necrosis to hepatic carcinoma, depending on time and dose. The aim of
cetamide administration on the masseter muscle. Twenty
CPE-1486
A new approach in the development of dengue biosensor by using hybrid nanocomposites and Cratylia
mollis lectin
of the 4th week, and fragments of masseter muscle were
collected for routine histopathological analysis, and frozen
1. Centro Acadêmico de Vitória - Universidade Federal de Pernam-
studies. Ten pictures were taken of each slide of masseter
49
Experimental Pathology and Health Sciences
ric analysis was performed with software ImageJ, for the
The analysis of the results of the morphologic analysis of
masseter muscle on tiocetamide administration, shown no
Pediátrica – HUOC - BRAZIL.
Background: SLE is an autoimmune multifactorial disease
TNF may play an important role in the development and
clinical manifestation. TNF promoter polymorphism has
-
CPE-1488
Toxicological evaluation in mice with extract of Phyllanthus tenellus Roxb
Silva, T.C.L.1; Santos, L.S.1; Ferraz, I.C.1; Silveira,
M.M.B.M.1; Lorena Júnior, E.J.P. 1; Veras Filho, J.2; Higino, J.S.2; Araújo, E.C.1 ; Souza, I.A.2 ; Amorim, E.L.C.2
1. Dep. de Enfermagem - Universidade Federal de Pernambuco
(CAV) – Brasil
2. Dep. de Farmácia - Universidade Federal de Pernambuco – Brasil
ology: We realized a case–control study contein 59 lupus
cases and 76 lupus-free adults controls. Diagnosis of SLE
Rheumatism Association criteria (ARA), and disease activat the Rheumatology Clinic of the Hospital das Clínicas
and the differences were analyzed using the ”Odds Ratio”
and “G” test, respectively; a p value of less than 0.05 was
calculated using the software BioEstat 5.0. Results: When
-
groups, the results showed that the frequency of A allele
Methodology: We performed the second testing method
repeated exposure of mice within 45 days evaluating the
mice with daily for signs of toxicity. Results: The shortas nontoxic. However the species showed adverse effects
we analyzed whether genotypes are associated with clinical
-
a depressant effects including: drowsiness, prostration, dehowever the group that received the higher dose experi-
CPE-1489a
respiratory rate, prostration, piloerection, swollen muzzle,
Crohn Disease
plant for use depending on its concentration. Conclusions:
“natural” are safe is frequent, thinking that they present no
risk of toxicity, can lead to serious consequences, side effects, interactions and poisonings unprecedented, making
it necessary measures of education and effective informaplants and their derivatives.
Keywords: P. tenellus, LD50, subchronic.
CPE-1489
Polymorphism in region promoter of Tumor Necro(SLE) susceptibility
Angelo, H.D.S.1,2; Fernandes, W.N.3; Silva, H.A 4; Asano,
N.M.J 5; Lins, O.G.6; Maia, M.M.D1; Muniz, M.T.C2,7;
Souza, P.R.E1,2.
1 - Laboratório de Genética-Bioquímica e Sequenciamento de DNA
- UFRPE - BRAZIL;
2 –Instituto de Ciências Biológicas - UPE - BRAZIL;
3 – Programa de Pós-graduação em Patologia - UFPE - BRAZIL;
4 - Departamento de Genética – UFPE - BRAZIL
5 - Departamento de Medicina Clínica da UFPE - BRAZIL;
6 – Departamento de Neuropsiquiatria da UFPE – BRAZIL;
7 - Laboratório de Biologia Molecular, Centro de Oncohematologia
50
gelo, H.D.S.3,4 , Souza, P.R.E3,4 , Torres, D.O.C.5, Martinelli, V. 6 , Lima, F.E.6 ,Telles, A. M. S.7 , Melo-Junior,
M. R.7,8.
1- Programa de Pós-graduação em Patologia - UFPE - Brazil
2- Centro de Ciências da Saúde – UFPE- Brazil
3- Laboratório de Genética-Bioquímica e Sequenciamento de DNA
– UFRPE – Brazil
4- Instituto de Ciências Biológicas - UPE - Brazil
5- Pronto Socorro Cardiológico de Pernambuco – UPE-Brazil
6- Hospital das Clínicas – UFPE- Brazil
7- Departamento de Patologia- UFPE-Brazil
8- Laboratório de Imunopatologia Keizo Asami – LIKA/UFPE,
Brazil
and disease severity, prognosis and monitoring the outcome of therapy and avoid invasive procedures.
late these measurements with the anatomical and clinical
forms of the disease.
July 2011, 49 patient outpatient services of Gastroenter-
2011 5 (2)
disease from clinical, radiological, endoscopic and histo-
man kala-azar among patients undergoing serological test
-
Carlos Diniz located in the city of Patos - PB. Material and
Methods: This is a documentary study, retrospective with
quantitative approach in which records of 802 patients
with suspected human leishmaniasis from various regions
-
in light of the literature concerning the matter. Throughout the study, the secrecy of the sample was preserved,
according to Resolution No. 196-96, which regulates rethrough the records was: origin of patients, age, sex and
results of serological tests. Results: It was found that out
from these exams.
clinical evolution and the chances of reicidiva.
Keywords: Crohn disease; ultra sensitive C-reactive protein(US-CRP); erythrocyte sedimentation
rate(ESR).
CPE-1490
Serological surrvey of antibodies IgG anti-Toxoplasma gondii in cattle from Zona da Mata of Pernambuco state, Brazil
Guerra, N. R.1; Alves, B. H. L. S.1; Monteiro M. F. M.1;
Sandes, H. M. M.1; Santana, V. L. A.2; Souza, M. M. A.3;
Santos, A. S.1; Silva C. M. S. L.1; Mota, R. A.1; Alves, L.
C.1
1. Universidade Federal Rural de Pernambuco, Recife, Brasil
2. Fiscal Federal Agropecuária- MAPA
3. Fiscal de Defesa Agropecuária- ADAGRO
Background: Toxoplasmosis is a zoonotic infection trans-
erly, has high rates of mortality. Thus, it is concluded that
these results are relevant to the disease frequency in the
study area, as well as comparative studies carried out later.
Keywords: Leishmania - disease – Paraiba
CPE-1519
Bioactivity of extracts obtained from aerial parts of
Croton grewioides
Fernandes, H. M.B.1; Pessoa, D.R. 1; Viana, W.P. 1; Medeiros, V.M. 1; Oliveira Filho, A.A1; Fernandes, H.B. 1;
Tavares, J. F. 1; Silva, M. S. 1; Diniz, M. F. F. M. 1; Cas1. Laboratory of Pharmaceutical Technology, Federal University of
Paraíba- Brazil
Introduction: The genus Croton is one of the largest of
goondi oocysts in cat feces, and also through consumption of raw or undercooked meat with cysts and drinking unpasteurized milk. However, cattle are less suscep-
-
IgG anti-Toxoplasma gondii in cattle from Zona da Mata
from 427 cattle herds located in 13 counties from Zona
evaluated the toxicity of hexane extract, chloroform, ethyl
acetate and ethanol of Croton grewioides through the
sults showed the Chã de Alegria County with highest
counties and the contamination of food and water with T.
goondi oocysts from cat feces are discussed.
Keywords: Toxoplasma gondii, Serological, Cattle.
CPE-1507
Frequency Of Human Kala-Azar In The Interior Of
Paraíba
for 24 h and then the survivors larvae were counted to delications were done for each concentration and the experishrimp test for hexane extract, chloroform, ethyl acetate
-
Faculdades Integradas De Patos, Patos – Pb
sites of the genus Leishmania. In the vector, the parasites
on extracts of hexanico, acetate de etila and cloroformico,
intense and widespread parasitism of cells of monocytic
macrophage system, with massive hepatosplenomegaly.
51
Experimental Pathology and Health Sciences
Phytochemical studies with them are underway to isolate
Keywords: Croton grewioides, Artemia salina, Bioactivity.
of the enzyme cyclooxygenase) or tempol (superoxide dismutase mimetic). Results: The HFD-group had a higher
CPE-1519a
Wanderley University Hospital
Fernandes, H.M.B.1, Oliveira Filho, A.A.1, Fernandes,
H.B.2, Oliveira, T.L.1
1 - Health Sciences Center, Federal University of Paraíba - Brazil
2 - Biomedicine Course, Integrated Faculties of Patos - Brazil
versity Hospital Lauro Wanderley. Material and Methods:
This study is an epidemiological survey, developed from
Hospital Lauro Wanderley. Records were used, containing
results of parasitological examinations of 7844 patients,
internal or who sought outpatient care. The survey was
conducted from tests made in the period January to Despecies of parasites, as well as their frequency. Results:
-
test). In the vascular reactivity evaluation, the HFD group
ute to endothelial dysfunction in high-fat diet-induced
Keywords: Obesity; Endothelial Dysfunction; Metabolic Syndrome.
CPE-1520a
Association between diabetes mellitus and nephropathy: A retrospective study of patients treated at the
University Hospital of Recife
1Silva, O. A.; 1Martins, J.C.M.; 1Silva, J.J.T.; 2Silva, M.R.
1. Departamento de Enfermagem da Universidade Federal de Pernambuco
2. Departamento de Enfermagem da Universidade de Pernambuco
leading cause of chronic renal failure (CRF) in various
greater insight into the association of these two patholo-
contained parasites. Among the positive results, helminths
to clinical impairment of patients with CRF in Pernamventions, on the means of contamination, prevention and
Keywords: Intestinal parasite, Helminths, University
Hospital Lauro Wanderley
CPE-1520
Tempol or indomethacin improves vascular dysfunction in obese rats
1Silva, O. A.; 1Queiroz, D. B.; 2Souza, V. N.; 2Fonseca, L.
A.; 1Duarte, G.P.
1. Departamento de Fisiologia e Farmacologia da Universidade Federal de Pernambuco,
2. Departamento de Fisiologia e Farmacologia da Universidade Federal de Alagoas
medical disorders that, when occurring together, increase
researched the medical records of 98 patients with CRF
were divided into two groups: NDM Group, patients with
diagnosis of CRF and MD Group, patients with diagnosis of CRF and DM. The data analyzed were related to
epidemiological and clinical characteristics of the patients.
the NDM group and 40 in DM group. In this, there was
a mean age higher than that in the NDM group (60 vs.
51 years, respectively). The mortality rate was higher in
DM). In the latter, the evolution to dialysis was faster. The
had lower urine output. Conclusions: It is shown that the
environmental factors, especially the consumption of distudy was to evaluate the effect of high fat diet (HFD)
on vascular reactivity of young rats. Methodology: Eightweek-old male Wistar rats were divided into two groups
assigned to receive, with free access, two kinds of diet: a
entire period of dietary intervention. At the end of the
diet period (6 weeks), we evaluate the vascular relaxation
52
action on health and therapeutic adaptation to this type
of patient.
Keywords: Chronic Renal Failure, Diabetes mellitus,
Morbidity, Mortality.
CPE-15
Epidemiological Study Of People With Acute Barber
Bug Fever On Northern Of Brazil
Gomes, A. C. L.1; Da Silva, P. K. F. 1; Silva, P. K. F. 1;
Soares, L. H. S. 1; França, M. J. D. M. 1; Costa, B. G. S. 1;
Lorena Júnior , E. J. P. 1; Silva, K. D. M. 1; Melo, M. S. 1;
2011 5 (2)
Patriota ,H. S. 1; Melo, G. P. 1
1. Universidade Federal De Pernambuco- Ufpe/Cav -Brazil
Patriota ,H. S. 1; Da Silva, P. K. F. 1; Silva, P. K. F. 1 Moura,
T. M. L. 1; Gomes, C. C. S. 1 ; Oliveira, R. A. A. 1; Bar1. Universidade Federal De Pernambuco- Ufpe/Cav -Brazil
cruzi, from the “sting” of triatomines, known in Brazil as
immunity individuals or evolve to a chronic form. With
-
-
premature realization of elective cholecystectomy laparoscopic. Even with the advance in laparoscopic procedure,
the patients are not risk free of show post discharge altoms of a patient with choledocholithiasis, systematize and
American Nursing Diagnosis Association). Methodology:
It is a descriptive study of the kind case report, done in a
controlling BBF actions on the last years, emerging the
need of rethink the used strategies in the disease control,
adoptions of integrative politics and effective actions of
epidemiological vigilance with special attention to the major risk group cited in this research.
Keywords:
Barber bug fever, Epidemiological,
Northern Region.
to 15th of July of 2011, where a patient with secondary
choledocholithiasis was accompanied to iatrogenic stenolecystitis. Results: During the period of data collect, were
track the diagnostics according to the patient general state
needs; (3) acute pain; (4) impaired tissue integrity; (5) risk
CPE-1522
Prenatal Care: A Tool For Early Detection Of Hepatitis B Virus In Pregnant Women
Santos, LS1; Moreira, LR1; Souza, GML1; Souza, VP1; Bezerra, MRE1; Santos, RP1.
1. Universidade Federal de Pernambuco, Brasil.
portance the nursing assistance and systematization has
a virus directly cytopathic, causing different clinical responses that can vary since no of symptoms to fulminant
hepatitis, chronic hepatitis, cirrhosis and hepatocellular
carcinoma featuring the pregnant women that are a high
risk group to transmission of this disease, the need for an
-
CPE-1524
Pharmacognostic study and preliminary toxicological
Pseudobombax marginatum (St.-Hil.) Robyns
Veras Filho, J.1; Basto, S.R.L.2; Ferraz, I.C.3, Peixoto Sono, J.S.1; Silva, T.C.L.3
1. Dep. de Farmácia - Universidade Federal de Pernambuco – Brasil
2. Dep. de Biologia - Universidade Federal de Pernambuco (CAV)
– Brasil
3. Dep. de Enfermagem - Universidade Federal de Pernambuco
(CAV) – Brasil
Background: Studies ethnopharmacological on Pseu-
knowledge and attitude of pregnant women in the city of
B in pre-natal care. Methods: This study was carried out
to virus research. Conclusions: The implementation of
educational practices to prenatal care on the transmission
vention and early detection, controlling the spread of the
disease and reducing mortality.
keywords: Gestantes; Hepatite B; Cuidado Pré-natal
CPE-1523
Secondary Choledocholithiasis To Stenosis Iatrogenic Of Biliary Ways: Understand The Pathology And
Implementing The Nursing Assistance
necessary the general knowledge of the disease, keeping in
Keywords: cholecystitis; cholecystectomy laparoscopic; choledocholithiasis.
to analyze the antioxidant activity and preliminary assess
the toxicity against the larvae of Artemia salina Leach.
Methods: Chromatographic methods were used according to Wagner and Bladt (1996) is aimed to highlight the
methods of Folin-Ciocalteu (1927) to determine the phe-
53
Experimental Pathology and Health Sciences
anthocyanins, aglycones and glycosidic anthraquinones,
phenolic acids), coumarins and terpenoids. The groups
that stood out were the diversity of terpenoids and phe-
CPE-1526
Preliminary screening of biological and toxicological
Ziziphus joazeiro Mart
;
1
cies. With regard to antioxidant activity, the sample showed
1. Dep. de Enfermagem - Universidade Federal de Pernambuco
(CAV) – Brasil
2. Dep. de Farmácia - Universidade Federal de Pernambuco – Brasil
Background: Ziziphus joazeiro Mart. popularly known
their green leaves, even in periods of drought. The high
content of saponins found, the species provides a deterfor cleaning teeth, skin whitening and skin diseases. It
and antioxidant.
Keywords: P. marginatum, phytochemicals, Artemia
salina.
CPE-1525a
Basic Techniques Of First Aid In The School Environment: A Short Course Given To County Teachers
In The City Vitória De Santo Antão
Galindo Neto, NM¹; Oliveira, RAA1; Muniz, MLC1, Santos I.T.S1, Costa, AB1, Santos ECB1, Silva ACA2, Santos
LRC2
1, Nursing Department. Federal University of Pernambuco,
Vitória Academic Center, Brazil.
2.Faculdade de Enfermagem Nossa Senhora das Graças-FENSG
Universidade de Pernambuco- UPE
Background: The school environment is prone to various
kinds of accidents and the very nature of school age favors
such occurrences. In emergency situations, the evaluation
ing preliminary test of toxicity. Methodology: To evaluNCCLS standards. We used strains of Gram-positive and
-
-
professionals to have access to the injured pupil, so the
knowledge of right course of action on the part of such
professionals has great relevance for the successful initial
care, while assistance does not arrive or so that appropriaid techniques taught in the school environment for teach-
Keywords: Z. joazeiro, antimicrobial, Artemia salina.
which occurred during the Regional Meeting of SBPC in
CPE-1531
Importance of vaccines against Human papillomavirus – HPV
theoretical and practical approaches. The facilitators were
teachers and students of the undergraduate nursing course
as participants. We used audiovisual resources, data, mannequin cardiopulmonary resuscitation for simulation and
tory, the teachers demonstrated that they had learnt the
theoretical content and also gave satisfactory performances in hands-on sessions. Conclusions: Trainings for teachoptimize the chance of life and the reduction of sequelaes
support during the training of education.
Keywords: Health education, accidents in the school
environment, teacher training
54
A.P.B.4; Tavares, I.F.5; Melo, L.N.T.6
1. Departamento de Ciências Farmacêuticas – UFPE/ Brazil
2. Departamento de Ciências Biológicas - UFPE/ Brazil
3. Departamento de Enfermagem - UFPE/ Brazil
4. Faculdade Integrada da Vitória de Santo Antão- FAINTVISA/ Brazil
5. Departamento de Ciências Biológicas, Universidade de Pernambuco – UPE/ Brazil
6. Departamento de Ciências Ambientais – UFPE/ Brazil
the family Papillomaviridae, is a DNA virus from sexual
transmission that is highly prevalent in man and woman
and cause anogenital warts and cancer. Sexual contact is
er ways such as the fetal-maternal, self-inoculation from
-
2011 5 (2)
jectives: The aim of this study was to perform a survey of
and the importance of vaccines against human papillomavirus - HPV in this context. Methodology: For that, updatprojects that address this issue. Results: Currently we have
from VLPs derived from a single viral protein, L1, which is
a major structural protein of the virus, which contains its
immunodominant epitope. These vaccines protect against
so that the immune system recognizes it and avoids a viral
vented from installing themself. Conclusions: According
clusion: The results demonstrate the need of an epidemiological control and greater vigilance to reduce the impact
health. Keywords: Hepatitis B. Epidemiology. Public
Health.
CPE-1532a
Epidemiological Situation Of Schistosomiasis Manson In Brazil During The Period 2008 To 2011
Costa, B.G.S.¹; Lorena Júnior, E.P.¹; Melo, M.S.¹; Silva,
1. Departamento de Enfermagem - UFPE/ Brazil
2. Departamento de Ciências Biológicas – UFPE/ Brazil
3. Departamento de Ciências Farmacêuticas – UFPE/ Brazil
4. Faculdades Integradas da Vitória de Santo Antão - FAINTVISA/Brazil
5. Departamento de Enfermagem – Universidade de Pernambuco,
UPE/ Brazil
Background: Schistosomiasis is an infectious disease
in young patients associated with an effective screening
family, gender Shistosoma. This represents an endemtoday.
Keywords: Human Papillomavirus. Vaccines. Cervical carcinomas
transmission occurs through the long human with water
containing cercariae and clinical outcome may vary from
zil. Methodology: For this, a survey was conducted in the
CPE-1531a
Analysis Of The Occurrence Of Hepatitis B In Pernamcountry in the period 2008 to 2011, taking into considerCosta, B.G.S.¹; Lorena Júnior, E.P.¹; Melo, M.S.¹; Silva,
area of residence and evolution). Results: During the study
1. Departamento de Enfermagem - UFPE/ Brazil
2. Departamento de Ciências Biológicas – UFPE/ Brazil
3. Departamento de Ciências Farmacêuticas – UFPE/ Brazil
4. Faculdade Integrada da Vitória de Santo Antão - FAINTVISA/ Brazil
5. Departamento de Ciências Biológicas - Universidade de Pernambuco/ Brazil
6. Departamento de Ciências Ambientais - UFPE/ Brazil
7. Departamento de Enfermagem – Universidade de Pernambuco/
Brazil
sion: The results show the necessity of a program aimed
at the epidemiological control of disease, since it is an important endemic parasitic.
Keywords: Schistosomiasis. Epidemiology. Shistosoma mansoni.
padnaviridae, genus Hepadnavirus. It represents a major
CPE-1533
Epidemiological Situation Of Yellow Fever In Brazil
During The Period From 2007 To 2010
-
-
cases of viral hepatitis has increased in recent years causing great expense to care for infected patients. According
to the World Health Organization (WHO), more than two
1. Departamento de Ciências Biológicas – UFPE/ Brazil
2. Departamento de Enfermagem - UFPE/ Brazil
3. Departamento de Ciências Farmacêuticas – UFPE/ Brazil
4. Faculdade Integrada da Vitória de Santo Antão -FAINTVISA/Brazil
5. Departamento de Ciências Biológicas- Universidade de Pernambuco – UPE/ Brazil
ducted a survey at the Sistema de InfoNational Informa-
Flaviviridae, genus Flavivirus that infects the cells of our
in the period 2008 to 2011, taking into consideration the
of residence and clinical forms of the disease). Results:
During the period of the study, 3497 cases were reported,
55
Experimental Pathology and Health Sciences
of yellow fever cases reported in Brazil. Methodology: We
data collection, refering to the yellow fever cases reported
during the period from 2007 to 2010, examining some
education, evolution, and year). Results: During this peserved that the affected individuals are usually young men,
carrying out agricultural activities, extractive, practitioners
of eco-tourism areas of risk where step into forest areas
without vaccination. Conclusions: Thus, there is a need to
make an expansion of coverage, especially for those individuals most affected, such as strengthening of vector
control actions in the municipalities located near areas of
transmission.
Keywords: Yellow fever. Aedes aegypti. Haemagogus.
CPE-1533a
that integrate the environmental issue.
Keywords: Trypanosoma Cruzy. Chagas Disease.
Epidemiology
CPE-1534
During The Period From 2006 To 2010
Almeida, M.C.¹; Costa, B.G.S.¹; Santos, C.R.C.¹; Gomes,
C.C.S.¹; Lorena Júnior, E.J.P.¹ ; Silva, K.D.M.¹; Pereira,
1. Departamento de Enfermagem-UFPE/ Brazil
2. Departamento de Ciências Biológicas, Universidade de Pernambuco- UPE/ Brazil
Background: Venomous animals are those that have glands
in Brazil are spiders, snakes, scorpions, caterpillars and
authorized, so the only guarantee that there are no accidents is the prevention of its occurrence. Accidents caused
During The Period From 2006 To 2010
1. Departamento de Ciências Biológicas – UFPE/ Brazil
2. Departamento de Enfermagem - UFPE/ Brazil
3. Departamento de Ciências Farmacêuticas – UFPE/ Brazil
4. Faculdade Integrada da Vitória de Santo Antão - FAINTVISA/Brazil
5. Departamento de Ciências Biológicas - Universidade de Pernambuco/ Brazil
6. Departamento de Ciências Ambientais - UFPE/ Brazil
7. Departamento de Enfermagem – Universidade de Pernambuco/
Brazil
Background: Chagas disease is a consequence of hu-
fusion, vertical, oral, accidental and vector, the last one
Methodology: We used the National Information System
gas disease reported during the period 2006 to 2010, ex-
For this study we used the Sistema de Informação Nacio-
and lethality of the victims. Results: During this period,
325.028 cases were reported, there was a predominance
promotion of actions to control and health education as
well as decrease the severity, frequency of sequelae and
lethality through the appropriate use of serum therapy.
Keywords: Venomous animals. Accidents . Public
health.
CPE-1535
Current Situation Of Leprosy In Pernambuco State
Costa, B.G.S.¹; Almeida, M.C.¹; Gomes, C.C.S.¹; Lorena
Júnior, E.J.P.¹ ; Silva, K.D.M.¹; Pereira, M.A.S.¹; Melo,
1. Departamento de Enfermagem-UFPE/ Brazil
Leprosy is an infectious disease of slow evolution, and
During this period it was noted 671 reports of the injury,
It mainly affects the skin and nerves, leaving the person
search invigorate the need to carry out regional studies, so
they can take measures aimed at controlling every aspect
of the population, thus, to have an adequate development
of actions to control Chagas disease, is extremely important that Primary Care Teams and the Family Health teams
incorporate into their work process surveillance activities
56
weakness and paralysis in the areas where the affected
study aimed to identify the prevalence of leprosy cases in
-
2011 5 (2)
collection for the years 2008 to 2011. We analyzed some
of residence, deaths, cure and treatment dropout). During
the period 11,961 cases were reported, of which 103 died
-
3- Student of Biomedicine Maurice of Nassau – Brazil
Introduction: Several authors consider that the assessment
of the adequacy of the sample is the most important isolated component in quality assurance in Pap smear (Papanicolaou smear) result. The Bethesda system in the original
versions included three categories of adequacy: satisfactory, unsatisfactory and a category designated initially “less
need for regional studies, raising account some aspects that
treatment, requiring a resolute surveillance that provides
greater control of the disease or at least the early discovery
rosy and giving an opportunity to cure the sick.
Keywords: Leprosy. Hansen’s bacillus. Mycobacterium leprae
CPE-1536a
Epidemiologic Review about Leishmanoise Tegumentar Cases in Children from the City of Vitória de
Santo Antão
Melo,G.P.¹; Patriota,H.S.¹; Morais, L.L.M.¹; Silva, P.K.F.¹;
da Silva, P.K.F.¹; Santos,L.S.¹; Ferraz, I.C.¹; Melo, M.M.B.¹;
Simôes, P.T.¹; Melo, R.O.¹; Almeida, M.C.¹
1. Department of Nursing. Universidade Federal de Pernambuco
- UFPE, Brazil.
Introduction: Because it is a phase where the organism is
dominates in the line of reasoning, the child many times
presents chances to acquire parasitic diseases compared
to adults. In this context the Leishmanoise Tegumentar is
gaining room over this social class, disease characterized
pal vector the straw mosquito, from the gender Lutzomyia,
appearance of papules may evolve to ulcers with granular
-
cases of Leishmanoise Tegumentar in children from 0 to
category and are now designated as “satisfactory” or “under to determine the prevalence in relation to the sample
(satisfactory or unsatisfactory) the results of cytopathology in municipal health service in Campina Grande – PB.
Methodology: This was a descriptive exploratory study
with a quantitative approach, the data were collected on
sults were as follows: of the 300 study results showed that
to educational lectures for professionals who deal with the
collection and staining of cytological material.
Keywords: Papanicolaou smear, satisfactory, unsatisfactory
CPE-1538
Anorectal Citology As Screening Method Of Anal
Intraepithelial Neoplasia (Ain) In Hiv-Negatives
Women
Castor, L. G1.; Nunes, M. J. G2.; Castor, M. G. F. C3; Medeiros, A. L. P. B4.; Bruneska, D5.; Pontes-Filho, N. T6.
1. Laboratório de Imunopatologia Keizo Asami/LIKA/UFPE,
Brasil.
2. Universidade Federal de Pernambuco/Programa de Pós-Graduação em Patologia, Brasil
3. Universidade Federal de Pernambuco/ Hospital de Câncer de
Pernambuco, Brasil
4. Laboratório de Imunopatologia Keizo Asami-LIKA, Brasil
5. Universidade Federal de Pernambuco/Centro de Ciências Biológicas, Brasil
6. Universidade Federal de Pernambuco/Centro de Ciências da
Saúde, Brasil
man Papillomavirus (HPV) can lead to the development
transition zone of the anal canal it may lead to pre-cancer
-
is realized the importance of creating political integrative
strategies as campaign, projects and programs aiming to
get rid of this pathology from this infant age, present in
the research as of most risk.
Keywords: Epidemiology; Children Health; Leishmanoise Tegumentar
of anal cytology as screening method to detect anal intraepithelial neoplasia (AIN). Methodology: 48 patients,
attended in Pelvis Service from Cancer Hospital of Peranal canal and transition zone. After detection of clinical
lesions, anorectal cells were collected with gynecological
CPE-1537
Quality Of Cytopathology In Campina Grande –Pb
Oliveira, Stéphanny Sallomé Sousa1; Araújo, Thereza
-
Giovanni Tavares3.
1- Academic Biomedicine Maurice of Nassau – Brazil
2- Lecturer School of Nursing Paulista – Brazil
and good preservation of the samples. These samples
57
Experimental Pathology and Health Sciences
this context it is important to search for new therapies.
cacy of the method. Additional studies with larger sample
and comparison with other methods may determine the
methanol in the same proportion to the extract methanoKeyords: Anal Citology, AIN, Human papillomavirus
rotaevaporation. The dry extracts were re-suspended with
CPE-1539
Antimicrobial activity of endophytic fungus Trichoderma harzianum isolated from Hancornia speciosa
Gomes
Chagas, M.B.O.1; Santos. I.P. 1; Silva, A.S. 1; Silva, C.M.A.
M.S. 1; Lima, V.L.M. 2
1. Department of Mycology - UFPE, Brazil
2. Department of Biochemistry - UFPE, Brazil
Background: Hancornia speciosa Gomes, known as manginterior of plants and among these, fungi, have attracted
mL. Were then added 30 µL of the extracts on disks (5 mm
in diameter) sterile paper and transferred to Petri dishes,
in triplicate, containing agar-Müeller-Hinton (MHA) me-
and is considered positive when the halos were more than
10 mm in diameter. Results: The ethanolic extract did not
other microorganisms showed activity, varying the average
phytic fungus Trichoderma harzianum isolated from the
(PDA) medium contained in Petri dishes, in triplicate, and
ouraud (SAB) medium. After this period, were fragmented
halos of 16 mm to 31 mm in diameter. Conclusions: The
extract methanolic showed more active against the test mipromising for future studies in order to evaluate its use in
infection control.
Keywords: H.speciosa; nosocomial infections.
CPE-1540
Síndromes Hipertensivas Induzidas Pela Gestação E
Repercussões Perinatais: Um Estudo De Revisão
triplicate containing agar - Müeller-Hinton (MHA) medi-
12.5 mm to 33 mm in diameter. Conclusions: The endo-
1. Acadêmica do curso de Bacharelado em Enfermagem pela Faculdade Maurício de Nassau.
2. Curso de Bacharelado em enfermagem da Faculdade Maurício de
Nassau. Recife-PE, Brasil.
Introdução: entre as doenças maternas que ocorrem no
período gravídico, a hipertensão induzida pela gravidez é
considerada uma das que mais efeitos nocivos provocam
no organismo materno, fetal e neonatal, presente em 10 a
tensivas induzidas pela gestação, seus fatores predisponen-
genic microorganisms. In future research, the results of
revisão de literatura de estudo transversal, descritivo. Para
tanto, foram consultados materiais impressos, como liv-
-
estudo, onde foram selecionados 30 artigos com período
H.speciosa;
ternos predisponentes para as síndromes hipertensivas
gestacionais e o desenvolvimento da mesma, repercutindo
moniae, Pseudomonas aeruginosa and the fungi Candida
SAB medium. After 48 hours were taken the readings of
halos were more than 10 mm in diameter. Results: All iso-
Keywords: Antimicrobial
T.harzianum.
activity;
CPE-1539a
Antimicrobial activity of extracts of stem bark from
Hancornia speciosa against the most common microorganisms in nosocomial infections
Chagas, M.B.O.1; Santos. I.P. 1; Silva, A.S. 1; Silva, C.M.A.
M.S. 1; Lima, V.L.M. 2
1. Department of Mycology - UFPE, Brazil
2. Department of Biochemistry - UFPE, Brazil
Background: The incidence of nosocomial infections is a
58
mostrou-se como principal fator de risco para seu desenvolvimento e neonatos pequenos para a idade gestacional
foi a principal repercussão perinatal encontrada durante a
sez de estudos referentes a essa patologia torna difícil o
acesso aos conhecimentos que poderiam prevenir tais
-
2011 5 (2)
mento adequado.
Palavras-chave: hipertensão gestacional; eclâmpsia;
pré-eclâmpsia.
CPE-1545
Analysis Of Secretory Activity In Rats Under The Effect Of Derivatives Phthalimides
Silva, T.L1;Jimenez, G.C1 ; Neto, J.F.S1; Souza, D.M.B1;
Antunes, R.V2.
1. Departamento de Morfologia e Fisiologia Animal – UFRPE,
Brasil.
2. Departamento de Química – UFRPE, Brasil.
Introduction: Phthalimides represent an important group
-
the intraperitoneal cavity. Animals in four groups received
a type-oxadiazole Phthalimides (A, B, C, D), and another
group served as control. At the end of nine days all animals were eviscerated after anesthetic depth with Xylazine
spectively (intramuscular). Then the liquid was removed
histopathological evaluation was performed on fragments
of the tumor, liver, spleen, lung and kidney. Result: It was
found that only Phthalimides A and B showed a tumor
tistically in the control group. Histopathologic evaluation
ter structural preservation compared to the control group.
effects of derivatives of oxadiazole-Phthalimides on the
secretory activity in rat ileum, particularly the participation
of chloride channels. Methodology: oxidiazole-ftalimídimechanisms of tumor development.
Keywords: Tumor, inhibition, Phthalimides.
24 hours of fasting, were anesthetized with ketamine +
-
CPE-1549
on growth of solid Ehrlich tumor
Pinto, F. C. H.1; Souza, D. A. S.1; Costa, P. M.1; Da Silva,
ileocecal six loops were formed, each received one type of
solution pH 7.2 in a volume of 20µL per compartment
R. D.2; Almeida, L. F.2; Tavares, S.2; Pinto, A. R.2
1. Federal University of Sao Joao Del Rei, Divinopolis, Brazil
2. Presidente Antonio Carlos University, Barbacena, Brazil
Background: Aloe vera, also known as the true or medici-
animal left as control (Ringer pH 7.2). After a period of
and magazines in your content evaluated, measuring up
chloride ions. Results: All derivatives produced ftalimidicos secretion characteristic shape. Derivatives ftalimídicos A, B and C strongly promoted vascular congestion,
ever, there are evidences that Aloe vera extracts present
genic effects of Aloe vera ethanolic extract on the solid
Ehrlich tumor was evaluated. Methodology: A total of
18 Swiss female mice, two months old, were divided into
three groups of 6 animals each: A, B and C groups. The
of chloride ions in concentrations similar, suggesting an
of enterocytes. Conclusion:-oxadiazole derivatives evaluated ftalimidicos seem to affect paracellular transport
of Aloe Vera ethanolic extract. Additionally, at the thirty
the arachidonic acid cascade.
Keywords: Phthalimides, secretion, rats.
CPE-1545a
Tumor Inhibition In Swiss Albino Mice Mus Musculus, Submitted To Derivatives Ftalamicos
Silva, T.L1;Jimenez, G.C1 ; Neto, J.F.S1; Souza, D.M.B1;
Antunes, R.V.2
1. Departamento de Morfologia e Fisiologia Animal – UFRPE,
Brasil.
2. Departamento de Química – UFRPE, Brasil.
Introduction: Cancer is a very common type of pathology
improvement aimed at early diagnosis and effective treatment, quality of life. The phthalimides represent a new
Ehrlich tumor cells (0.05 mL) on the footpad. The tumoral
ing a digital pachymeter. At the sixty day, the animals were
-
differences among tumors of the groups. The metastasis
in popliteal nodes of the tumor occurred in all animals.
Conclusion: Aloe vera ethanolic extract potentiated and
accelerated the tumoral growth. In such case, future studthe activity of the tumor.
Keywords: Ehrlich tumor, Aloe vera, histopathology.
CPE-1550
with four different types of synthetic derivatives of oxa-
mammary neoplasms in cats
E.L.4; Peixoto, C.A.4; Neves, C.O.5; Melo-Júnior, M.R. 5;
Porto, A.L.F.6
59
Experimental Pathology and Health Sciences
1. Centre for Animal Experimentation, Instituto Oswaldo Cruz,
Fundação Oswaldo Cruz, Brazil.
2. Veterinary Hospital, Universidade Federal Rural de Pernambuco, Brazil.
3. Laboratory for Clinical Research in Dermatozoonoses, Instituto
de Pesquisa Clínica Carlos Chagas, Fundação Oswaldo Cruz, Brazil.
4. Laboratory of Ultrastructure, Department of Entomology, Instituto Aggeu Magalhães, Fundação Oswaldo Cruz, Brazil.
5. Departament of Pathology, Universidade Federal de Pernambuco,
Brazil.
6. Department of Morphology and Physiology, Universidade Federal Rural de Pernambuco, Brazil.
Background: The vast majority of mammary tumors in cats
These tumors have poor prognosis, since such tumors are
-
the ovariectomized animals presented higher serum levels
-
ment. Conclusions: alpha-tocopherol supplementation
point of view histochemical and antigenic spontaneously
occurring mammary tumors in cats. Methodology: Thirtyhistological sections were stained with Masson Trichrome
and Periodic Acid Schiff (PAS) to evaluate the deposition
of collagen and glycosaminoglycans associated with tumors, respectively. To perform the immunohistochemistry
assays were used anti-Ki-67, anti-PCNA and anti-Bcl-2.
Keywords: alpha-tocopherol; ovariectomized rats; osteopenia
CPE-1554
one derivative in carrageenan-induced acute injury in
mice lung
of interstitial collagen and PAS + cells in mucinous cara higher markup to decreasing reactivity with the PCNA
marking is only for PCNA and Ki-67. Conclusions: The
data suggest that malignant neoplasms in cats have intense
proliferative activity, indicating a poor prognosis.
Keywords: Mammary neoplasia, Felis catus, Immunohistochemistry.
E.L.1; Donato, M.A.1; Silva, T.G.3; Lima, M.C.A.3; Pitta,
I.R.3; Galdino, S.L.3; Peixoto, C.A.1,2
1. Centro de Pesquisas Aggeu Magalhães - CPqAM/FIOCRUZ,
Brazil
2. Centro de Tecnologias Estratégicas do Nordeste- CETENE/
MCT, Brazil
3. Universidade Federal de Pernambuco/Departamento de Antibióticos - Brazil
Background: Peroxisome proliferator-activated receptors
CPE-1551
The Development Of Osteopenia In Adult Ovariectomized Rats
Schiavotelo, Nathalia L.1; Camargo, Vânia B.1; Santos,
Amanda M.1; Gonçalves, Francisca de A. M.2; Ruffoni,
1. Departamento de Morfologia e Patologia, Universidade Federal
de São Carlos, Brasil
2. Centro de Estudos Ambientais, Universidade Estadual Paulista,
Brasil
3. Departamento de Ciências Fisiológicas, Universidade Federal de
São Carlos, Brasil
Background: Several studies have aimed to identify aljectives: This study was designed to determine the effect
of alpha-tocopherol supplementation in the experimental development of osteopenia in adult ovariectomized
rats. Methodology: 36 Wistar rats aged 120 days were
Two weeks after ovariectomy, treatment was initiated with
days, or only the vehicle (sterile peanut oil, 0.2 ml). Ninety
days after treatment the serum levels of calcium, phosphorus and alkaline phosphatase were measured; the mineral
60
injetion at the level of the left sixth intercostal space. Saline
injected into the pleural cavity. At 4 h after the injection
pors. Lung fragments were processed for light microscopy
and immunohistochemical assays. Results: As expected, no
saline-treated animals. Immunohistochemical analyses did
cal analysis of the CAR group showed increased cellularity,
emphysema. The CAR group presented intense staining
for cyclooxygenase (COX-2) compared with control mice.
Increased COX-2 expression is associated with increased
levels of downstream enzymes required for prostanoid
program Gimp 2.6 software. Conclusions: According to
to clarify the role of RA-4 on the molecular mechanisms
2011 5 (2)
-
Six healthy volunteers each received a single oral dose of
0.5 mg digoxin, from Glaxo Wellcome S.A, Brazil. Blood
CPE – 1555
Antitumor effects of 8-methoxypsoralen in an in vitro
model of glioblastoma
1.50, 2.00, 3.00h, 4.00, 8.00, 12:00, 24:00, 48:00, 72:00,
tory.
M.L.V.2; El-Bachá, R.S.1
1. Laboratory of Neurochemistry and Celular Biology, Health Sciences Institute - UFBA - Brazil
2. Gonçalo Moniz Research Center - Fiocruz - Bahia - Brazil
-
1.819, 1.137, 0.774, 0.640, 0.357, 0.268, 0.263, 0.058, 0,
time, nevertheless the impact of technological advances
new therapeutic approaches, treatments and drugs are
roliferative and apoptosis-inducing activities of the furanocoumarin-derivate 8-methoxypsoralen (8-MOP), a drug
used for skin diseases treatment. Methodology: Rat glioma
trocytes were used in tests with increasing concentrations
and colony formation assay. Cell morphology was accessed
used for elucidation of the kind of cell death. Results:
-
(52.24), 17.7800 and 17.1480 (92,53), 10.1066 and 10.6012
(91,13), 0.1518 and 0,1493 (98,23) respectively. Concluin Linux software.
Keywords: Digoxin, Linux, pharmacokinetics.
CPE-1556a
Comparative evaluation of haemoglobin using N/10
hydrochloric acid versus automated method
Bezerra da Silva, J.F.1; Oliveira, TN1; Souza Filho1, S.D.;
Alves, A.Q.2; Alves, A.J.1
1. Departamento de Ciências Farmacêuticas. Universidade Federal
de Pernambuco, Brazil,
2. Faculdade Pernambucana de Saúde, Brazil
-
diameter of the colonies. In addition, 8-MOP increased
MTT assay, in GL-15 cells. Conclusions: 8-MOP presented
antiproliferative and apoptosis-inducing activities in glioma cells. Additionally, it did not affect normal astrocytes
to the extent that affected tumor cells. Then, 8-MOP is a
good prototype of new anti-glioma drug as well as a pos-
not have such equipment; they are expensive and require
-
-
Keywords: Cancer; psoralen; glioma
CPE-1556
Pharmacokinetics of a Single Dose of Digoxin in
Healthy Volunteers using Linux Systems
Oliveira, T.N.1; ALVES, A.J.1; ALVES, C.J.4; ALVES, A.Q.2;
GÓES, A.J.S.1 Carvalho Neto, J.L.3; Aquino, T.M. 1
1. Department of Pharmaceutical Sciences, Federal University of
Pernambuco Stat, Brazil,
2. Faculty of Health of Pernambuco Stat, Brazil,
3 Hospital Mermorial Guararapes, Brazil.
4 Maxiclinic Medical, Brazil
-
cardiotonic glycoside worldwide for the treatment of congestive heart failure and atrial arithmias. Even though it is
known as a drug with a narrow therapeutic index. Routine
two software. Methodology: The protocol complied with
the provisions of the Declaration of Helsinki (1964) and
compared to automated method. We consider this as clinias the mean difference ±2 SD and the calculated lower and
dl respectively. Both these suggest that the methods agree
the Ethics Committee from Hospital Memorial Guara61
Experimental Pathology and Health Sciences
Keywords: haemoglobin, hydrochloric acid
CPE-1557
Prediction Of 3d Structure Of Protein Messenger Of
Ovarian Immunosuppressants Ascaris Suum
ga, C.G.O1; Pontes-Filho, N T3; Souza, V.M.O1.
1. Laboratório de Imunopatologia Keizo Asami - LIKA/UFPE,
Brazil.
2. Department of Biochemistry, UFPE, Brazil.
3. Department of Pathology, LIKA/UFPE, Brazil.
Background: Extract of Ascaris suum adult worms has
proteins with regulatory activities that can modulate the
immune response to antigens homologous or heterolo-
have assessed the longevity of this enhancement and
whether it is dependent on the administration route. For
S. mansoni), on the 60th day had their estrus synchronized
infected mothers (Control). For analyzing of the administration route three groups were added: animals controls
ing weaning in two doses: 0,12µg(SEA.1) or 1,2µg(SEA.2).
The third group received oral SEA, after weaning (21 days)
at the dose of 6 µg(SEA-oral).On the 45th day, all ani21th, 28th, 35th and 68th days post-immunization. It was
of this extracts with immunoregulatory action in a model
the study was the prediction of protein structural Messenger immunosuppressive Ovarian (PMIO) of A. suum and
residual analysis of phosphorylation targets. Methodology:
group was more evident from the 28th day. However, for
the animals which had received milk from infected, fourfold IgG1 level was detected yet on the 8th day and it kept
until the 28th day. For the animals sensitized with SEA,
National Center for Biotechnology Information (NCBI),
was analyzed for the phosphorylation sites (program NetPhos - prediction of phosphorylation on serine, threonine
animals. Regarding the titles of IgG2a, the increment was
(NetPhosk) then the structural prediction was made in
route, in the lactation phase, induced an early production
dues in most favoured regions (MFR), additional allowed
regions (AAR), generously allowed regions (GAR) and disallowed regions (DR). The model was also analyzed with
the IgG1 synthesis.
Keywords: Schistosomiasis experimental; Breastfeeding; SEA.
quality compared with protein structures deposited in the
Protein Data Bank (PDB). Results: Found 11 residues of
serine undergo phosphorylation (positions 29, 67, 77, 93,
124, 191, 286, 288, 290, 301, 397), 8 threonine (128, 178,
189, 200, 216, 246, 282 and 363) and 10 tyrosine (35, 61,
175, 186, 197, 222, 231, 269, 339 and 431). NetPhosk indicated the involvement of protein kinase C activation in
PMIO acting on the serine residue at position 124. The 3D
model generated showed high stereochemical quality with
overall quality has resulted in Z-score (-2.92) in accordance
netic Resonance. Conclusions: Study of the structure and
the mechanisms involved in activation of PMIO result in
knowledge for future immunotherapy strategies related to
autoimmune diseases.
Keywords: Ascaris suum; Protein; Immunomodulation.
CPE-1557a
Kinetics Of The Anti-Ovalbumin Antibodies Production In Adult Mice Sensitized With Antigens Of
Schistosoma Mansoni In The Breastfeeding
querque, M.C.P.A1; Costa, V.M.A.1; Souza, V.M.O1.
1. Laboratório de Imunopatologia Keizo Asami - LIKA/UFPE,
Brazil.
2. Biotherium of Centro de Pesquisa Aggeu Magalhães, Fiocruz,
Brazil.
Schistosoma mansoni infected pregnant women are common
in endemic areas. Expositions to parasitic component in
sponse to heterologous antigen. Experimental studies in
62
CPE-1559
New Candidates for anti-Schistosoma mansoni: Evaluation of Biological Activity
Santiago, EF1; Leite, ACL1; Moreira, DRM1; Oliveira,
SA2; Barros, AF 2; Silva, AL3
1.Laboratório de Planejamento e Química Medicinal - UFPE,
Brazil
2. Laboratório de Imunopatologia – FIOCRUZ, Brazil
3.Laboratório de Planejamento e Síntese de fármacos - UFPE,
Brazil.
of Africa have showed resistance to praziquantel, this is
still the drug of choice worldwide. In the new scenario,
it is necessary to study new drugs with activity schistosozone. Methodology: We used 50 mice of the strain Swiss
the portal system for the recovery of worms. The parasites
After a period of two hours of adaptation, three phenyl-
motor activity and mortality. Resultados: The molecule
-
2011 5 (2)
-
2. Academic Center of Vitória - Federal University of Pernambuco, Brazil
Background: The comet assay is a simple, sensitive and
quantitative method for measuring DNA single and strand
ics and process of DNA repair. Agricultural workers are
prone to occupational exposure to a variety of pesticides
which represents a potential risk to human health, includ-
Keywords: schistosomiasis, thiosemicarbazone, study
“in vitro”
CPE-1561
Vegetables Show DNA Protector Effect Even Contaminated With Pesticides: A Study By Micronucleus
Test
Silva, E. J.1; Dias, S. A.2; Freitas, E. M. S.2; Adam, M. L.2;
And Chagas, C. A.1
1. Postgraduate Program in Human Health and Environment,
Academic Center of Vitória - Federal University of Pernambuco,
Brazil
2. Academic Center of Vitória - Federal University of Pernambuco, Brazil
-
sonal Protection Equipments (PPE) in the damage detectogy: The study included 54 individuals, 31 farmers and 23
non-exposed controls. The farmers were evaluated in two
day-use of PPE during the same activities. DNA dam-
toxic damage, H2O2 was used as positive control. Regroup (ID-0.14±0.13; FD-10.38±10.58) and control (ID-
nation. Methodology: Eighteen Wistar rats were divided
Group 1 was water control, Group 2 received 1 daily dose
-
- ITEP - to identify what pesticides were present in these
to carry out the Micronucleus Test. Results: Many different
then were found in concentrations lower than the Accepton micronucleated leukocytes when compared to Group 2
(p > 0.05 - Kruskal-Wallis Test). Antioxidants, naturally
tween Groups 1 and 2. A non dose-dependence on that an2 and 3. Conclusions: Despite the presence of pesticides,
genic effect in the experimental design carried out.
Keywords: Agrochemicals; DNA Damage; Antimutagenicity.
CPE-1561a
Genotoxicity In Agriculture Workers Exposed To
Pesticides: Effect Of Individual Habits And Use Of
Personal Protection Equipments In Dna Damage
And Chagas, C. A.¹
1. Postgraduate Program in Human Health and Environment,
Academic Center of Vitória - Federal University of Pernambuco,
Brazil
(0.06 ± 0.06) and FD (4.37 ± 4.12) in the Post-PPE when
ney test). Such factors as Smoking, alcohol intake, time of
exposure, age and sex had no effect on the ID and FD in
all experimental groups (P>0.05). Conclusion: Our results
of DNA damage in farmers. The use of PPE reduced the
exposure to pesticides and consequently the damage was
repaired.
Keywords: Agrochemicals; Comet Assay; Biomonitoring
CPE-1563
Histopathologic Characterization Of The Neuromuscular Mutation Fraqueza (Frq) Induced By Enu
In Mice
Policarpo, A.C.F.1,2; Silva Silva, D.A.1; Massironi, S.M.G.3;
Guénet, J.L.4; Arantes, R.M.E.5; Godard, A.L.B.1
1.Laboratório de Genética Animal e Humana, Instituto de Ciências
Biológicas - Universidade Federal de Minas Gerais, Brasil.
2. Fundação Comunitária de Ensino Superior de Itabira –
FUNCESI, Brasil.
3. Departamento de Imunologia, Universidade de São Paulo, Brasil.
4. Unité de Génétique dês Mammifères, Institut Pasteur - França.
5. Laboratório de Neuro-Imuno Patologia Experimental, Universidade Federal de Minas Gerais, Brasil.
Mouse mutagenesis is a key tool for studying gene funcmouse colonies. The fraqueza mice mutant phenotype
mutation is deleterious and inherited as an autosomal
D1Mit320 (18.64 cM). Fraqueza mice present progressive
loss of motor coordination and muscle strength at two
weeks postnatal, associated with writhing and contraction
at 12 weeks postnatal. From the molecular and phenotypic
the gene Dystonin (Dst, Bpag1) as a candidate for the mu63
Experimental Pathology and Health Sciences
1. Health Sciences Center, Federal University of Paraíba - Brazil
at characterizing the mutant phenotype fraqueza through
techniques of complete necropsy, histological evaluation
of spinal cord and nervous and muscle tissues, and clinical
characterization of the phenotype. Morphometric analysis
evidenced smaller mean muscular citoplasmatic area in mutant mice compared to their paired controls evaluated at 3,
7 and 11 weeks postnatal. Analysis of histological sections
of muscles presented slight muscle atrophy in fraqueza
mice, with increase in endomysial conjunctive tissue and
slight vascular neoformation. There is also a slight increase
in nerves and spinal ganglia cellularity and decrease in
terations. The results may indicate ultrastructural injury
neurodegenerative diseases and we intend to validate this
model for the development of therapies for human dis-
tives: This study aimed to assess the incidence of intestinal
parasites in patients treated at the Polyclinic of Cajazeiras,
PB. Methodology: The parasitological tests of the samples
Municipal. The 517 samples were analyzed in the period
mentation technique, in order to determine the frequency
of protozoan cysts and helminth eggs and larvae. Results:
tive. As the protozoa and the species most frequently was
-
Keywords: animal models, mutagenesis, neurodegenative disease, histopatology.
-
CPE-1565
Incidence Of Intestinal Parasites In The Sandy
Beaches Of Paraíba
Oliveira Filho, A. A.1; Fernandes, H. M. B.1; Alcântara, N.
D. F.1; Freitas, F. I. S.1
1. Health Sciences Center, Federal University of Paraíba - Brazil
Background: The infectious and parasitic diseases are still
gions and poorer populations, however, despite the great
technological advances of the high standard of education,
good nutrition and good health, even developed countries
dence of intestinal parasites, especially protozoa cysts that
sures such as improved sanitation and hygiene education
continued as well as new studies parasitological, after the
implementation of these measures, if necessary, and are
few studies that address the levels of infection of intesti-
the month of May 2010, totaling 112 samples. The parasitological tests used in this study were analyzed accordthe spontaneous sedimentation technique and the method
Keywords: Parasitological tests, Polyclinic of Cajazeiras, Intestinal parasites
CPE-1567
Histopatological Changes Induced By Bothrops Leucurus Venom On Mice Skeletal Muscle After Experimental Poisoning
1. University of Pernambuco, Brazil. 2. Federal University of
Pernambuco, Brazil. 3.San Francisco University, Brazil. 4.State
University of Campinas, Brazil
-
the presence of at least one species of intestinal parasite.
Samples of wet sand had a higher rate of positivity for the
helminths. Conclusions: The high rate of parasitic forms
research reveals the need for improvements, through the
cases, amputations. In the present study we examined the
prevention and health education, as an infection with intestinal parasites can cause damage not only to individuals
the mice gastrocnemius muscle. The anesthetized mice received the intramuscular injection of venom (100 µL, 80
of the population.
Keywords: Parasitological tests, Paraiba Coast, Intestinal parasites.
group) on the dorsal-lateral region of the right paw. After
3hs, 6hs, 1 day, 3 days, 7 days and 21 days after inocula-
CPE-1565a
Evaluation Of The Incidence Intestinal Parasites In
Patients Treated At The Polyclinic Of Cajazeiras, Pb
Oliveira Filho, A. A.1; Moura, D. V.1; Fernandes, H. M.
B.1; Freitas, F. I. S.1
64
light microscope and transmission electron microscope.
2011 5 (2)
The ultrastructural analysis showed the disorganization of
the sarcomeres and mitochondrial damage on the muscles within 24 h post-venom injection. The presence of
-
third day after venom injection. At 21th day we noted the
restoration of muscle cytoarchitecture except for the presence of interstitial edema. Our results proved the myotoxic
action of B. leucurus venom and that the satellite cells participated to the muscle regeneration processes.
Keywords: Bothrops leucurus, Muscle Skeletal, Myotoxicity
-
CPE-1569
Keywords: L-fucose, vessel, prostate.
Chromogenic In Situ Hybridization In Breast Cancer
Samples
Silva Filho, J.L.Q.1; Cartaxo, M.F.S.1; Lima, L.R.A.1; Cavalcanti, C.L.B1; Ramalho, E.A.V.F.1; Beltrão, E.I.C.1,2
1. Laboratório de Imunopatologia Keizo Asami – LIKA/UFPE,
Brazil; 2. Departamento de Bioquímica, CCB/UFPE, Brazil.
Background: Breast cancer is the second most common
CPE – 1571
Luminescent Probes for Breast Tissues: the Use of
Europium (III) Cryptate Conjugated to Lectins in
Diagnosis
Brandão, J.M.1; Bandeira, A.R.G.1, 2; Vasconcelos, J.L.A.1;
Cavalcanti, C.L.B.1; Júnior, S.A.2; Beltrão, E.I.C.1, 3
1 Grupo de Pesquisa Biomarcadores no Câncer - LIKA/UFPE,
Brasil.
2 Departamento de Química Fundamental – DQF/UFPE, Brasil
3 Departamento de Bioquímica – CCB/UFPE, Brasil
cer in women. In this context, the study of some markers
may help the understanding disease progression, as well
as planning the most appropriate therapy. c-myc gene is
involved in cell differentiation, apoptosis, angiogenesis
and immortalization playing central role in the tumorigen-
pathological parameters in CDI samples. Methodology:
tion of the c-myc was detected in 2 out of 10 cases. These
two cases were of women under 50-year-old and tumours
fonodal invasion with histological grade III. Among negative patients 5 were older than 50-year-old, 7 had tumours
for diagnosis of cancer has conducted research in pursuit
of this goal. Histochemical studies have shown that quanface glycoconjugates during neoplastic processes. Lectin
histochemistry is a tool that can auxiliaries in the diagnosis
of these pathologies, regarding changes in their saccharide
content. Europium (III) cryptate, macromolecules with lustudy proposed the use of the conjugate lectins-europium
cocode of glycoconjugates on the cell surface of trans-
and only one histological grade I, 2 were grade II and 5
were grade III. Conclusions: The present results show that
than 50 years with high histological grade and linfonodal
invasion. Indicating that this oncogene is associated with
aggressive tumors.
Keywords: Hibridization in situ; c-myc; breast cancer.
CPE-1570
Expression of L-fucose in blood vessels as biomarker
for human prostate diseases
Vasconcelos, J L A1, Brandão, J M1; Cavalcanti, C L B1;
Lima, A L R1; Beltrão, E I C1,2.
1. Laboratório de Imunopatologia Keizo Asami – LIKA/UFPE,
Brazil
2. Department of Biochemistry – CCB/UFPE, Brazil
Background: The prostate gland is the male reproductive
-
Results: Normal tissues showed no emission in all samples.
FIB samples showed staining only for Con A-Eu(III)crip
Eu(III)crip (average emission of Con A, WGA and PNA
Samples luminescence intensity showed different patterns
clusions: This work suggests the use of lectins-Eu(III)crip
Fucosylation is a mechanism which functions to insert the
seen as one important factor in the development of varisies diagnosed with Benign Prostatic Hyperplasia (BPH -
sis as an auxiliary tool.
Keywords: europium cryptate, lectin, breast tissues.
CPE-1573
Mycobacterium leprae DNA detection in nasal secretion of patients with leprosy from the Center of
National Reference in Sanitary Dermathology Dona
65
Experimental Pathology and Health Sciences
Libânia (CDERM) – Fortaleza, Ceará
Marques, LCM 1; Lima, LNGC 1; Assis, LC 1; Dantas, JCP
1; Kerr, LRS 1; Frota CC¹.
1. Laboratory of Mycobacteria, Medicine School, Federal University
of Ceará, Brazil
rium leprae, which dissemination occurs through the nasal secretion and affects mainly the skin and nerves. Ceará
ranks as tge 10th in overall detection rate in the country.
-
-
in nasal secretion from individuals with leprosy. Methwho were cared in the Center of National Reference in
cases from the total of participants. In general, men had a
women the highest positivity was from 31 to 40 years old
developing leprosy than women. Conclusion: The sensitivity of the nested PCR for the RLEP2 region is very high
histochemistry using haematoxylin and eosin in skin diagnosis.
Keywords: chemiluminescence, histochemistry, lectins, carbohydrates, cutaneous tumors.
CPE-1576
Experimental invasive pulmonary aspergillosis: histopathological analysis of lungs from immunocompetent and neutropenic animals infected by Aspergillus
fumigatus strains with different patterns of production of elastase
Silva, R.L.H.1; Rosa-Milani E.2; Brunaldi, M.O.1; Maffei,
C.M.L.2
1. Departament of Pathology and Forensic Medicine. Faculty of
Medicine of Ribeirão Preto - University of São Paulo.
2. Departament of Cellular and Molecular Biology and Pathogenic
Bioagents. Faculty of Medicine of Ribeirão Preto - University of
São Paulo.
Background: Invasive Pulmonary Aspergillosis is an opwhich affects mainly immunocompromised patients. Neutropenia is a prerequisite for hematogenous dissemination
Keywords: nasal mucus, leprosy, PCR
CPE-1575
Chemiluminescent Quantitative Lectin Histochemistry Determination Of Cutaneous Tumors Glycophenotype
Lima, L.R.A.1, Bezerra, M.F.1, Silva, L.P.B.G.1, Almeida,
S.M.V.1, Cavalcanti, C.L.B.1, Beltrão, E.I.C.1, Carvalho
Júnior, L.B.1
1. Laboratório de Imunopatologia Keizo Asami (LIKA), Universidade Federal de Pernambuco, Cidade Universitária, Recife, Pernambuco, Brasil
Background: Chemiluminescence assay is a promising and
effective technique for quantitative analysis of proteins,
associated with invasiveness is the production of elastase
the lungs of immunocompetent and neutropenic animals
and strong (1753) production of elastase. Methodology:
ally with monoclonal anti Gr-1 (induction of neutrophil
after different periods (24, 48 and 72 hours). Immunosections that were stained with Hematoxylin and Eosin
in their glycosylation patterns when compared to their
normal counterparts. Lectin histochemistry is an effec-
(W). Results: Histopathologic analysis of lungs stained
-
and its functional consequences regarding glycophenotype
work aimed to develop a chemiluminescent lectin histo-
24 and 48 hours post-infection in neutropenic animals
in cutaneous tumors. Methodology: Con A and PNA were
Sephadex G-25 chromatography. Lectin-AE conjugates
were assayed for haemaglutinatin activity and chemilumiwith conjugates, separately, and chemiluminescent reac-
66
sue fungal load. During this period there was no evidence
These results indicate that neutropenia is an essential factor in the invasiveness of the fungus and suggest that the
virulence of A. fumigatus is multifactorial. Financial Support: FAPESP and FAEPA
Keywords: Experimental invasive pulmonary asper-
2011 5 (2)
gillosis, neutropenia; elastase.
CPE-1577
Establishment and Partial Characterization of a Cell
Line from a Human Glioblastoma
Lima, R.M.F1; Sampaio, G.P.2.; De Oliveira, D.M1.; ElBachá, R.S.1
1. Laboratory of Neurochemistry and Cell Biology - Federal University of Bahia, Brazil
2. Laboratory of Immunology and Molecular Biology - Federal University of Bahia, Brazil
-
The cell line ESP10 was derived from a sample of tumor
protein (GFAP), oligodendrocyte transcription factor 2
(olig-2), proliferating cell nuclear antigen (PCNA) and huhistochemistry, immunocytochemistry and cell cytometry.
cellular pleomorphism. DTCP average was 30 hours. The
SIONS: This new cell line of GBM showed a predomi-
aggressiveness and invasiveness. Characterization studies
Keywords: human glioblastoma, characterization, cell
line
CPE-1579
New acridine derivative conjugated to lectin as chemiluminescent probe for detection of tumor biomarkers
Almeida, S.M.V.1; Lima, L.R.A.1; Bezerra, M.F.1; Silva,
L.P.B.G.1; Cavalcanti, C.L.B.1; Lima, M. C. A.3; Galdino,
S.L.3; Pitta, I.R.3; Beltrão, E.I.C.1,2; Carvalho Júnior,
L.B.1,2
1. Laboratório de Imunopatologia Keizo Asami (LIKA), Universidade Federal de Pernambuco (UFPE), Brasil.
2. Departamento de Bioquímica, Centro de Ciências Biológicas
(CCB) – UFPE, Brasil
3. Departamento de Antibióticos, Centro de Ciências Biológicas
(CCB) – UFPE, Brasil.
Background: There is an increasing interest and demand
with conjugate, separately, and chemiluminescent reaction
accomplished. Results: All analyzed tumour types, KA
Even showing a weaker chemiluminescent emission than
served with DMAE. These results indicate the use of IPKey-words: chemiluminescent, acridine derivative,
skin cancer
CPE-1580
mavirus In Anal Canal Samples Of Hiv-Negatives
Women
Nunes, M. J. G1., Ekert M.H.F2., Castor, L. G3.; Castor,
M. G. F, C4.; Bruneska, D5.; Pontes-Filho, N. T6.
1. Programa de Pós-Graduação em Patologia/UFPE/CCS, Brazil
2. Programa de Pós-Graduação em Biologia Aplicada à Saúde/
UFPE/LIKA, Brazil
3. Laboratório de Imunopatologia Keizo Asami/LIKA, Brazil
4. Hospital de Câncer de Pernambuco, Brazil
5. Departamento de Bioquímica/UFPE/CCB, Razil
6. Departamento de Patologia/UFPE/CCS, Brazil
Background: Human papillomavirus (HPV) comprises
more than 100 viral types, some of which infect the anogenital tract and lead to the development of Anal Intraepithelial Neoplasia (AIN). The persistence of HPV infection
is considered a risk factor for development of squamous
sample using polymerase Chain Reaction (PCR) and microarray. Methodology: The study was conducted with 26
patients, attended in Pelvis Service from Cancer Hospital
cells samples were collected from anal canal. Total DNA
was extracted for HPV detection through of PCR, using
ray using PapilloCheck® HPV-screen DNA-chip to identify 06 genotypes low-risk and 18 genotypes high-risk. Results: The molecular diagnosis through PCR detected the
PapilloCheck® analysis revealed HPV6 (low-risk) as the
ated the potential application of a new acridine derivative
(IP-81) conjugated to Concanavalin A (Con A) lectin as
G-25 chromatography. Con A-IP81 conjugate was assayed
for hemagglutination activity and chemiluminescence (ex-
with different HPV genotypes. Among negative samples
Conclusions: The results showed that PapilloCheck® diagnostic was effective to identify HPV in anal samples, and
67
Experimental Pathology and Health Sciences
progress to cancer.
Key-words: Anal Intraepithelial Neoplasia (AIN), human papillomavirus, genotyping
CPE-1580a
Histomorphologic Analysis Of The Heart Oh Rats
Submitted Malnutrition To Different Ages
Da Costa, C.P.4; Pontes-Filho, N.T.3
1. Master student from Programa de Pós-Graduação em Patologia/
UFPE/CCS
2. Programa de Pós-Graduação em Farmacologia/UFPR, Brazil
3. Departamento de Patologia – CCS/UFPE, Brazil
4. Departamento de Fisiologia e Farmacologia, UFPE, Brazil
Background: The normal function of an organ or of a
system depends of adequate nutrients intake. As several
uate the morphological and histological changes of heart
-
of 30 minutes per 3 weeks). At the age of 35-45 days, aniwas colleted, weighed, measured and processed for histology. The sections, stained with haematoxylin and eosin,
were captured (Motic) and analyzed (Image J) in order to
calculate mean diameter (100x) of gastrocnemius muscle
mean±standard deviation. Body weights (g) in the day of
for ArS, 126.53±17.25 for WE and 143.95±12.74 for WS.
respectively: 0.4881±0.0766, 0.0041±0.0002, 1.93±0.16
for ArE; 0.6859±0.1087, 0.00468±0.0004, 2.13±0.15 for
ArS; 0.6110±0.1396, 0.0044±0.0006, 2.28±0.36 for WE;
0.6357±0.0731, 0.0042±0.0003, 2.03±0.12 for WS. Mean
Diameter (µm) was 30855±2481 for ArE, 35784±3037
for ArS, 31670±2656 for WE and 28442±6140 for WS.
Conclusions: According to these results, physical exercise
of L-Arginine leads to muscle hypertrophy, independently
muscle weight and length.
Keywords: L-Arginine, exercise; skeletal muscle
period. The histogical analysis was performed in transversal sections of ventricles stained with Hematoxylin-Eosin
(HE) and Picrosirius Red. The morphological data was
the cardiomyocytes from 3 and 5 months age rats from
MG group. However, the changes were more intense in 5
CPE-1581a
Effects Of Low Frequency Tens With Electrodes On
Acupoints On Cold-Induced Pain
Melo, M.P.M¹.; Montenegro, E. J. N¹
1.Federal University of Pernambuco, Brazil
ences negatively the activities of daily living of an individual. Nowadays, many therapies are used to relieve the
pain, especially transcutaneous electrical nerve stimulation
uate the effect of acupunture-like TENS (low frequency)
located on acupoints TA 5 and CS 6 and the effect of
sis areas replacing the injured heart tissue, indicating repair
process. Conclusion: The results revealed that multifactorial malnutrition interfered in cardiac organogenesis during
post-weaning development, leading to histomorphological
mance in adulthood.
Key-words: malnutrition, heart, rat
CPE-1581
Effects of amino acid L-Arginine and physical exercise on skeletal muscle of young rats
Melo, M.P.P¹.; Vasconcelos, A.C.S.¹; Monteiro, H.M.C¹.;
L.M.S.S¹.
1. Federal University of Pernambuco, Brazil
Background: Physical exercise can promote many adaptations in skeletal muscles. In addition, the amino acid L-Arginine is related to improvement in exercise performance
of L-Arginine and physical exercise during development.
Methodology: 24 Wistar males rats were divided into four
groups according to orogastric administration of L-Argi-
68
genders, randomly allocated, were divided into 2 groups:
TENS with electrodes on acupoints TA 5 and CS 6 (A
group) and the sham TENS (P group). In group P, the
electrodes were placed on points not related to the induced
with cold water (2-40C) during 20 minutes in order to inment (Electroanalgesy) during 20 minutes. The latency
and after the treatment. These parameters were statistically
sults were expressed as mean±standard error. The latency
± 4.72 during, 36.29 ± 3.12 after treatment. The latency
± 6.41 during, 44.92 ± 7.93 after treatment. Conclusions:
According to these results TENS on acupoints TA 5 e
threshold, during and after the treatment, comparing them
therapy in treatment of algesic processes.
Keywords: acupunture-like TENS, placebo, acupoints.
CPE-1582
Association between the type of cervical injury and
2011 5 (2)
Human Papillomavirus genotype in Olinda, Brazil
jo, R.F.F.4; Pontes-Filho5, N.T.; Lima-Filho, J.L.4; Bruneska, D.4.
1. Programa de Pós-Graduação em Biologia Aplicada à Saúde/
LIKA/UFPE
2. Programa de Pós-Graduação em Patologia/CCS/UFPE
3. Programa de Pós-Graduação em Biologia Aplicada à Saúde/
LIKA/UFPE
4. Departamento de Bioquímica/CCB/UFPE
5. Departamento de Patologia/CCS/UFPE
Introduction: According to the World Health Organization, HPV infection is the major risk factor for develop-
-
area. The data were compared using the Mann-Whitney
mammary gland, the cellular proliferation was increased
of cases. Currently the Pap test (Pap smear) is considered
effective in early diagnosis and prevention of invasive
term has slight proliferative effect on the epithelial cells
als and Methods: The cross-sectional study was conducted
with 241 women, ranging from 18 to 60 years, attended in
underwent the examination and those cytology specimens
Results: Through the Pap test it was shown that only 7
tween the type of cervical injury and genotype showed
that HPV16 was the most prevalent when associated with
low-risk genotypes 6, 11 and 35 were associated with lowgrade lesions, also in young patients. On the other hand
genotypes 52, 66 and 82, considered high risk, were associated with atypical cells, conferring risk of evolution to
cervical injury. HPV52 was the most prevalent in samples
Keywords: tibolone, mammary gland, histomorphometry
Sources of research support: OFFICILAB, CAPES,
CPE-1583a
Mammary Gland Histomorphometry of Rats Treated
With Estradiol Valerate And Norethisterone Enanthate
Guzmán-Silva, MA 1; Henriques, HN 1; Couto BB 1; Azeredo VB 2; Pantaleão, JAS 3.
1 Pathology Department, 2 Nutrition and Dietetics Department, 3
Maternal and Child Department, Fluminense Federal University,
Niteroi/RJ, Brazil.
-
Conclusions: The high prevalence of the HPV16 in young
in their responses to diverse ratios of these two steroids.
prevention strategies to avoid cervical cancer, thus improving on treatments.
Keywords: Papillomavirus, cervical injury, genotyping.
CPE-1583
Histomorphometric Changes In Mammary Glands
Of Female Rats Treated With Tibolone
Guzmán-Silva, MA 1; Henriques, HN 1; Carvalho, ACB 1;
Pantaleão, JAS 2.
1 Pathology Department, 2 Maternal and Child Department, Fluminense Federal University, Niteroi/RJ, Brazil.
hormone replacement therapy and the exact mechanisms
involving sexual steroids in the mammary cancer risk are
not well-known.
Aim: Analyze the mammary parenchyma of ovariectotime.
Methods: Bilateral ovariectomy was performed on 14 rats
weighing 250g. After 30 days, they were randomly divided
cervical displacement. Histological sections of thoracic
matoxylin-eosin and the histomorphometric analysis was
performed on images of each histological section merged
with a 100-dot grid to estimate the area fractions for each
-
tomorphometry.
Methods: Fifteen Wistar female rats, aged eight weeks and
weighing in average 250g were randomly divided in three
of Mesigyna® (norethisterone enanthate and estradiol
of sterile water. Treatments were performed on days 0,
7, 14, 21, and 28 of the experiment. Thoracic mammary
logical sections were stained with haematoxylin-eosin and
histomorphometric analyses were performed on images
of each histological section merged with a 100-dot grid.
Points coinciding with the desired structure were counted allowing to estimate the area fractions for each rat as
acini fraction in the mammary gland (DAFM), epithe-
Results: The thoracic mammary gland showed an increase
69
Experimental Pathology and Health Sciences
the administration of norethisterone enanthate and estraferentiation and enlargement of the lumen, which leads to
Keywords: estrogen, progestogen, mammary gland,
histomorphometry.
Sources of research support: OFFICILAB, CAPES,
CPE-1585
Swainsonine measurement in milk of cows poisoned
the lectin histochemistry analysis of a nervous system and
was milled, mixed with commercial food and served to the
lar and epithelial cells gave positive reactions to Triticum
vulgaris, succinylated Triticum vulgaris, Lens culinares,
Concanavalia ensiformis, Pisum sativum, Ricinus communis, Arachis hypogaea and Phaseolus vulgaris erythroagglutinin. The positive reactions to these lectins indicates
acetyl-neuraminic acid. Conclusions: It is concluded that I.
Mendonça, F.S.3
1. Faculdade de Medicina Veterinária, Universidade de Cuiabá,
Brazil
2. Poisonous Plant Research Laboratory, United States Department
of Agriculture, USA
3. Departamento de Morfologia e Fisiologia Animal, Universidade
Federal Rural de Pernambuco, Brazil
study aimed to quantify swainsonine in milk of cattle poiMethodology: One adult cow naturally poisoned and two
lactating adult cows, and their suckling calves of 2 months
were used in the experiments. The detection and quantitation of the swainsonine in milk were accomplished using
reversed phase highperformance liquid chromatography.
Results: The lesions on poisoned animals were consistent
ease. The average concentration of swainsonine in milk
Keywords: Plant poisoning; storage disease; lectin
histochemistry
CPE-1589
Investigation of DNA from Mycobacterium leprae
present at collections of water in the municipalities
of Juazeiro and Crato, Northeast Brazil
Macedo, M. L. B.¹; Holanda, M.V.¹; Oliveira, T. E. S.¹;
1. Laboratory of Mycobacteria, School of Medicine, Federal University of Ceará, Brazil
2. Department of Geology, Federal University of Ceará, Brazil
Introduction: Leprosy is a chronic infectious disease
genetic factors, nutritional status, BCG vaccination and
source of infection, such as water, soil and animals. The
-
swainsonine in the milk. However, under these experimental conditions, the concentration of this alkaloid is not
sosomal storage disease in calves.
keywords: cattle, poisonous plant, lysosomal storage
disease, alkaloids
M.leprae present at samples of water reservoirs in endemic
areas in the municipalities of Juazeiro do Norte and Crato,
from nine water tanks, totaling 45 samples. The samples
were collected from ponds, streams, rivers and recreational
resorts. DNA of M. leprae was extracted with PowerWater
formed using “in-house technical”. The product consisted
CPE-1585a
Lectin-histochemichal analysis of lysosomal storage
disease induced by Ipomoea verbascoidea (Convolvulaceae) in goats
Norte and two from Crato. The low positivity may have
Correa, F3; Colodel, E.M.4
1. Departamento de Morfologia e Fisiologia Animal, Universidade
Federal Rural de Pernambuco, Brazil
2. Faculdade de Medicina Veterinária, Universidade Federal do Rio
Grande do Sul, Brazil
3. Hospital Veterinário, CSTR, Universidade Federal de Campina
Grande, Brazil
4. Departamento de Clínica Médica Veterinária, Universidade Federal de Mato Grosso, Brazil
Background: Lysosomal storage diseases result from an
jectives: The aim of this paper is to report the results of
70
Conclusion: This study indicates the presence of nucleic
acids from M. leprae in these collections of water. Therefore, these reservoirs may represent a source of infection
in community.
Keywords: environmental water; Mycobacterium leprae; DNA
CPE-1604
Immunohistochemistry analysis of the Ciclooxygenase-1 in wound healing submitted to different treatments
Martins Neto, V.1; Lopes, C.F.B. 3; Santos, H.B. 2; Thomé,
1. Laboratório de Biologia Celular e Mutagênese, CCO/UFSJ,
2011 5 (2)
Brazil
2. Laboratório de Processamento de Tecidos – LAPROTEC,
CCO/UFSJ, Brazil
3. Curso de Bioquímica – CCO/UFSJ, Brazil
Background: The cyclooxygenase (COX-1 and COX-2)
acts in the prostaglandins synthesis, molecules that play
skin repair. Conventional treatment with nonsteroidal anti-
convulsion.Results: The doses chosen regarding the Artemisia vulgaris had a latency dilated after the administration of pentylenetetrazol when compared to the diazepam
higher than the standard.Conclusion: The methanolic extract suggests an anti-convulsive action due to the fact
action of medicinal plants, commonly used to treat skin
ferent treatments, associating the expression of cyclooxygenase with the cicatrization process. Methodology: After
injury, a total of seven animals per group were treated
throughout nine days with water (G1), plant extracts of
Plantago sp (G2) and Siparuma sp (G3), and collagenase
ointment (G4). Samples of tissue from the wound area
ted to immunohistochemistry for COX-1. Analyses were
performed using an optical microscope and the Axio Vision software 4.8 Rel. Results: The immunolocalization of
COX-1 was predominant in the cytoplasm of epithelial
cells in G1, G2 and G3. In these groups there was diffuse
searches of new anti-convulsive drugs.
Keywords: Artemisia vulgaris; Anti-convulsing.
two cell layers. In G4 the immunostaining occurred in con-
System. Continuing Education (CE) function is essential
the groups treated. Conclusions: The results suggest that
the treatments did not differ in stimulating the synthesis
of Cox-1 in the skin repair. Therefore, it is necessary to
continue the investigation of the expression of Cox-1 and
Keywords: Cox-1; cicatrization; immunohistochemistry.
CPE-1605
Anticonvulsant Evaluation of Artemisia vulgaris using diazepam as standard and pentylenetetrazol and
convulsing drugs such as lidocaine
CPE-1606
Family Risk Scale as a tool to train and upgrade of
the CHAs of the Family’s Health Strategy in the
Wasteland rural city
Silva, T.H.L.1; Medeiros, J.H.1; Silva, D.M.1; Silva, D.C.A.1;
R.T.C.A1; Silva, L.G.A.1 ; Santana, P.R.1
1. Federal Universtiy of Pernambuco, Brazil / Victoria Academic
Center- UFPE / CAV, Brazil
2.Federal Universtiy of Pernambuco, Brazil
Background: The Family Health Strategy (FHS) was de-
trainees, to encourage them to work through update tools
and methodologies that meet these principles. The Community Health Agents (CHA) is part of the FHS, with an
important role in upgrading local health indicators. The
records of `Form A` (record of the family and their peculiarities). This scale is a guiding instrument for prioritithese datas is part of the CHA`s function, even the data
sional to update Form A. Methodology: It was requested
of Form A. Was chosen randomly one of the CHA and
M. E. R.¹ ; Silva, A. R. ¹; Soares, P. H. S.¹ ; Alves, J. O.¹ ;
Farias, F. C. V. M.¹ ; Almeida, E. R.¹
1.Laboratory of Psychopharmacology and psychoactive drugs and
their toxicology - Federal University of Pernambuco, Brazil
Introduction: The genus Artemisia (Asteraceae, Anthemid-
high economic value in some regions, such as food, ornamental plant, as fodder in the African steppes; in alternative medicine it has analgesic, antispasmodic, anticonvulsant, tonic, sedative, digestive, anthelmintic and regulating
menstruation.
Goal: This study aims to evaluate the anti-convulsing potential of the Artemisia vulgaris through a methanolic exSwiss mice were used. They were divided in three groups
for each compulsive drug, each group had six animals usvulsing factor an extract of the dosage was given to its
group and after a 60 minutes period the animals received
the effects of the convulsive drugs chosen (pentylenetet-
constant updating of Form A. The information generated
The reference choosen in this work was the FRS proposed
patory, demonstrated commitment to handling the FRS.
essential to seek innovative ways to respond the challenges
of an EC.
Keywords: Continuing Education, Public Health,
Family Health Program.
CPE-1606a
Recurrent urinary tract infection due to inappropriate use of antibiotic by health professionals: clinical
case report
Medeiros, R.T.C.A.¹; Soares, N.S.¹; Damasceno, T.Q.¹;
Silva, D.M.1; Silva, D.C.A.1; Lira, M.C.C.1
1. Federal Universtiy of Pernambuco, Brazil/ Centro Acadêmico
de Vitória – UFPE/CAV, Brasil
71
Experimental Pathology and Health Sciences
in the urinary system. It affects individuals of any age,
patients admitted to a university hospital in Recife. Methodology: A convenience sample was composed of patients
admitted to a university hospital in Recife - PE. It is a case
study, carried out from simple interview and consultation
33, graduated in pharmacy, was diagnosed with recurrent
-
units of Escherichia coli sensitive only to ertapenem and
ceftriaxone. The patient had severe side effects to ertapenem, ceftriaxone was administered for 14 days. Conclusions:
not only among laymen.
Keywords: Anti-Bacterial Agents, Recurrence, Urinary Tract Infections
shows higher sensitivity, our results demonstrates that
primers targeting gyrA region are also very sensitive. Conclusion: These results show that single-step PCR targeting gyrA gene is a potential alternative to detect M. leprae
RNA in environmental samples.
Keywords: environmental water; gyrA gene; Mycobacterium leprae
CPE-1616
Inhibitory effects of natural plant of Family Anacardiaceae on gelatinolitic activity of MMP-9
R.I.M.A.1
1. Laboratório de Biologia Celular e Mutagênese. CCO/UFSJ,
Brazil
2. Curso de Medicina. UFMG, Brazil
3. Molecular Oncology Research - Barretos Cancer Hospita , Brazil
Background: In the cancer, many of the target genes that
and late stages of disease and are required for metastasis
and angiogenesis such as matrix metalloproteases (MMPs)
that promote angiogenesis and invasive growth of cancer
fractionated extracts of a plant of the Family Anacardiaceae. Methodology: The ethyl acetate fraction, partition
from the crude extract, was eluted in the chromatographic
column and had their points grouped according to the
into solutions with the different extracts. To stimulate the
proteolytic activity of gelatinases, a portion of the gel was
CPE-1614
Comparison of two different PCR methods based on
RLEP and gyrA sequences for detection of Mycobacterium leprae in water environmental samples
Oliveira, T. E. S.¹; Holanda, M.V.¹; Macedo, M. L. B.¹;
-
1. Laboratory of Mycobacteria, School of Medicine, Federal University of Ceará, Brazil
2. Department of Geology, Federal University of Ceará, Brazil
-
samples with low amount of DNA. This region is found
repeated 28 times in the genome. However, the high sensisequences in environmental water samples. Methodology:
from Juazeiro do Norte and Crato municipalites, Northkept at 4C until processed. Five different samples were collected from each point of collection, resulting in total 45
samples. Total RNA was extracted using PowerWater RNA
-
Keywords: gelatinases; plant extract, Anacardiaceae
CPE-1618
Evaluation of antitumor activity in vivo Barbatic acid
isolated from Cladonia salzmanii (lichen)
Neto, M.P.C.1,2; Rocha, T.A.1; Andrade, C.A.S.1,3; Santos,
N.P.1,2; Silva, N.H.2; Falcão, E.P.S.1,2
1. Centro Acadêmico de Vitória- CAV/UFPE, Brazil
2. Laboratório de Química de Produtos Naturais, Departamento de
Bioquímica – UFPE, Brazil
3. Laboratório de Tecnologia em Biomateriais, Centro Acadêmico de
Vitória – CAV/UFPE, Brazil
Pharmaceutical agents from natural products have occupied a prominent position in the treatment of pathol-
RLEP and gyrA gene sequences respectively, were visual-
pounds produced is considered exclusive of mycota li-
72
2011 5 (2)
pancreatic hormones, anterior pituitary and adrenal is considered pathology of a chronic nature and rolling, setting
used as the experimental Sarcoma 180 tumor. The animals
and dental treatment. Periodontal disease is a chronic
vical dislocation and organs and tumors were removed and
prevalence levels, and the second leading cause of dental disease in the human population around the world. A
compared with the literature, in focus the fumarprotocetrárico acid isolated which showed an LD50 of around
an activator receptor it phosphorylated insulin, causing insulin can no longer activate these receptors, leading to the
-
Keywords: Antitumor activity, Barbatic acid, lichen
CPE-1620
Alpha-Tocopherol Supplementation Changes The
Camargo, Vânia B.; Santos, Amanda M.; Schiavotelo, Na-
in healing, vascular disease, dyslipidemia, among others.
Such complications have systemic repercussions, and how
Departamento de Morfologia e Patologia, Universidade Federal de
São Carlos, Brasil
-
of systemic health.
risk due to its capacity to decrease LDL oxidation, favor
arterial vasodilatation and modulate the activity of diverse
-
CPE-1624a
Chemiluminescent Detection Of Galectin-3 In Tumoral Tissue From Breast
Filho Magalhães, S. D. 1; Lima R. E. 1; Neto Silva, S. T. 1;
Silva, R. B. 1; Patu,V.J.R.M. 1
1. Faculdade ASCES
mune system and cancer. Immunohistochemical meth-
treated not ovariectomized (T-NO), untreated ovariectoly, every two days, or only the vehicle (sterile peanut oil, 0.2
rum concentration of Triglycerides (TG), Total Cholesterol (TC) and HDL-c using commercial kits. Results: It was
method.
gated to acridinium ester (AE) and the complex formed
tected.
and normal tissues than in IDC). These values showed to
these values were dramatically reduced when the tissue
year and at least 72 h, respectively.
clusions: alpha-tocopherol supplementation can prevent
in tissue. Breast tissues were used as a model.
drop in HDL-c and rise in not-HDL-c).
Keywords: alpha-tocopherol; ovariectomized rats;
CPE-1624
Periodontal Disease As A Risk Factor For Diabetes
Mellitus
Filho Magalhães, S.D.1; Araújo, R.V. S1
1. Faculdade ASCES, Caruaru, Brasil;
CPE-1631
Clinical study, diagnosis and epidemiology of onychomycosis cases reported in the Department of
Medical Mycology UFPE
Targino Silva, S.1; Lacerda Filho, A.M.1
1. Laboratório de Micologia Médica do Departamento de Micologia
da Univesidade Federal de Pernambuco – UFPE, Brazil
Background: Onychomycosis is a fungal infection of the
nail caused dermatophytes, yeasts or non-dermatophytic.
They appear in high frequency, although its clinical and
73
Experimental Pathology and Health Sciences
-
TCNM.
inappropriately. It is crucial the acquisition of clinical samCPE-1634
appropriate procedures for collecting and epidemiological
survey of patients treated at the Department of Medical
servation of clinical features of lesions that present in the
-
Vegetables Show DNA Protector Effect Even Contaminated With Pesticides: A Study By Comet Assay
Dias, S. A.2; Silva, E. J.1; Freitas, E. M. S.2; Adam, M. L.2;
And Chagas, C. A.1
1. Postgraduate program in Human Health and Environment,
Academic Center of Vitória - Federal University of Pernambuco,
Brazil
2. Academic Center of Vitória - Federal University of Pernambuco, Brazil
mainly species of Candida; Filamentous fungi were isolated in 22 cases, with emphasis on species of Fusarium
and Trichophyton. Of the patients 60 were women and 11
men, with ages ranging from 15 to 80 years. Conclusions:
teen Wistar rats were divided in three groups and were
-
treatments for onychomycosis, since this is a disease of
Keywords: Diagnosis; Fungi; Onychomycosis.
-
CPE-1633
Histochemistry Analyses of Sialic Acid Residues In
Non-Melanome Tumours Using SNA
Ferreira, S.A.1; Beltrão, E.I.C.1,2
1. Keizo Asami Immunopathology Laboratory (LIKA), Federal
University of Pernambuco (UFPE), Brazil.
2. Biochemistry Department, Biological Science Centre, Federal University of Pernambuco, Brazil.
(NMCT) are the most incidence malignant neoplasia in
ent types of tumours associated to stages of progression
and development of tumour cell. OBJECTIVES: This
socellular carcinoma (BCC) and spinocellular carcinoma
-
out the Comet Assay. Results: Many different pesticides
Daily Intake – ADI. Both parameters of analysis “Index
of Damage” - ID - and “Frequence of Damage” - FD showed that the Group 1 had a higher damage level than
cant difference was found in other comparisons. The difpromoted an antigenotoxic effect, protecting the animals
clusions: Despite the presence of pesticides, the ingestion
experimental design carried out.
Keywords: Agrochemicals; DNA Damage; Antigenotoxicity.
CPE-1634a
Mutagenicity In Agriculture Workers Exposed To
Biotin-Streptavidin-Peroxidase system was used to lectin
ing reagent. Haematoxylin was used for counter staining
and results evaluated using light microscopy. Cell staining
was evaluated according Dornelas (2009): (+) positive for
cells; (-) negative. Three random areas of tissues were ana-
BCC and SCC presented distinct patterns of sialic acid
residues expression in their cell surface glycoconjugates.
Results indicate that SNA can differentially recognize
auxiliary tool in histochemistry.
Key-words: Sambucus nigra agglutinin, sialic acid,
74
Of Personal Protection Equipments In Micronuclei
Frequency
Dias, S. A.1; Silva, E. J.2; Anjos, M. D. A1; Lima, C. D. S¹;
Freita, E. M. S.1; Adam, M. L.1; And Chagas, C. A.2
1. Academic Center of Vitória - Federal University of Pernambuco, Brazil
2. Postgraduate Program in Human Health and Environment,
Academic Center of Vitória - Federal University of Pernambuco,
Brazil
tigate the impact of exposure to genotoxins. Agricultural
workers are prone to occupational exposure to a variety
of pesticides which represents a potential risk to human
2011 5 (2)
the wound healing submitted to different treatments.
the use of Personal Protection Equipments (PPE) in micronuclei frequency were also evaluated in these workers. Methodology: The study included 54 individuals, 31
farmers and 23 non-exposed controls. The farmers were
evaluated in two moments: during pesticide application
-
R.I.M.A.1
1. Laboratório de Biologia Celular e Mutagênese, CCO/UFSJ,
Brazil
2. Laboratório de Processamento de Tecidos – LAPROTEC,
CCO/UFSJ, Brazil
Background: Mast cells have a central role in immediatetype allergy and also related to regulating the cutaneous
wound healing. The medicinal plants are used in the treat-
and stained with Giemsa. The frequency of micronucleated cells was performed in 1000 leukocytes per individual.
of micronucleated cells when compared to control group
(36.77±14.42 verses 11.60±7.55). The Post-PPE showed
Whitney) in when compared with the exposed group. Such
factors as alcohol intake, time of exposure, age and sex
had no effect on the frequency of micronucleated cells
in all experimental groups (P>0.05). However, smoking
to an increase of DNA damage in farmers. The use of
PPE reduced the exposure to pesticides and consequently
the damage was repaired. Smoking was the only paramenhance the DNA damage.
Keywords: Agrochemicals; Cytogenetic Damage;
Biomonitoring
CPE-1635
Adrenal Microanatomy Alteration Of Pinealectomized Rats At The Age Of 39 Days
Dias, S. M. S.1; Sousa, I. D. D.1; Santos, K. R. P.1
1. Universidade Federal de Pernambuco, Brasil
to different treatment. Methodology: After injury, a total of seven animals per group were treated throughout
nine days with water (G1), plant extracts of Plantago sp
(G2) and Siparuna sp (G3), and collagenase (G4). Samples
logical sections were acquired using an optical microscope
and the Axio Vision software 4.8 Rel. Statistical analysis
were expressed as means ± SEM. Results: In relation to
epithelialization until nine day, the G1, G2 and G3 groups
presented skin repair, while in G4 was not occurred. The
mast cells with normal and degranulated morphology were
recorded surrounding the wounds in all treatments. The
quantitative analyses of the mast cells population showed
the follow values: G1 (range 0.0 – 46.0; mean 17.7 ± 1.3),
G2 (range 0.0 – 45.0; mean 18.5 ± 1.3), G3 (range 3.0 –
42.0; mean 22.0 ± 1.5) and G4 (range 0.0 – 43.0; mean
and the other treated with collagenase. Conclusions: The
the pineal gland on the adrenal gland. Studies reported
the presence of melatonin receptors in the adrenal gland,
healing skin.
Keywords: mast cells, Plantago and Siparuna extracts, skin repair.
in the adrenal glands through the pinealectomy such as:
meaningful increase of the vascularisation, decrease of the
mone (ACTH), hypertrophy of the cortex layer, among
CPE-1637
Cytotoxicity of the extract of the leaves of microemulsion Schinopsis brasiliensis Engl. front macrophages cells
Castro, R.H.A.1; Batista, D.C.A.1; Coutinho, F.N.1; Saraiva, A.M.1; Silveira, W.L.3; Silva, T.G.2; Egito, E.S. T.3;
Psiciottano, M.N.C.1
1. Laboratory of Microbiological Analysis, Depto. Pharmaceutical
Sciences, UFPE, Brazil
2. Laboratory of bioassays for Research Department-Drug. Antibiotics - UFPE, Brazil
3. Dispersed Systems Laboratory. Faculty of Pharmacy - UFRN,
Brazil
the adrenal microanatomy alterations of pinealectomized
rats at the age of 39 days. Methodology: 20 Wistar rats
were used. They were separated into two groups: pinealectomized Sham rats and pinealectomized rats. At the end of
a-seven-month post surgery period the rats were anesthetized, euthanatized, and necropsied to remove the adrenal
were processed, cut at 3 micro centimetres thick, undermicroscopy. Results: Results showed a meaningful increase
of the adrenal gland vascularisation. The zona fasciculate
-
-
evaluated the cytotoxicity of microemulsion extraction
ciculate.
Keywords: Adrenal. Morphology. Pineal Gland.
front macrophage lineage cells. Methodology: The mac-
CPE-1636
Quantitative analyses of mast cells population during
The cells were resuspended in RPMI medium supplement75
Experimental Pathology and Health Sciences
tant was discarded and then the methanol extract of leaves
(MTT). Again taken the greenhouse for 2 hours (37 0C)
and then the culture medium along with the excess MTT
was aspirated and added dimethylsulfoxide wells. The opti-
low cytotoxicity against macrophage cells, encouraging result, although in vivo toxicological studies are needed to
reach further conclusions.
Keywords: Schinopsis brasiliensis Engl.; microemulsion, Cytotoxicity.
CPE-1637a
Cytotoxicity of the extract of the leaves of microemulsion Schinopsis brasiliensis Engl. front lineage
leukemic cells (K563)
Castro, R.H.A.1; Batista, D.C.A.1; Coutinho, F.N.1; Saraiva, A.M.1; Silveira, W.L.3; Silva, T.G.2; Egito, E.S. T.3;
Pisciottano, M.N.C.1
1. Laboratory of Microbiological Analysis, Depto. Pharmaceutical
Sciences, UFPE, Brazil
2. Laboratory of bioassays for Research Department - Drug. Antibiotics - UFPE, Brazil
3. Dispersed Systems Laboratory. Faculty of Pharmacy - UFRN,
Brazil
-
Brazil: an analysis of data obtained by PNDA (19982008)
Nascimento, R.A.F.1; Almeida, R.N.1; Canadá, M.N.S.1;
Carneiro, R.S.1; Santos, A.J.H.1; Sousa, A.B.1
1. Faculdade Santa Emília de Rodat
Background: In Brazil, the National Household Sample
jectives: This study aims to characterize the chronically ill
from the National Household Survey (health supplement)
of the years 1998, 2003 and 2008. Methodology: Data
were individuals who reported having at least one chronic
disease to PNDA. Data were collected in the system DAof patients with chronic diseases were selected the folcome per capita and coverage of health insurance. Results:
The PNDA recorded that DC prevalence in the population resident (PR) in Brazil in 1998, 2003 and 2008 were
2008 the percentage of DC was reported higher among
those who had an income of two minimum wages or more
years a higher growth in the population of DC than in
population of DC for the implementation of more effecKeywords: Chronic diseases; Prevalence.
study evaluated the cytotoxicity of microemulsion extracEngl. front lineage leukemic cells (K563). Methodology:
Cells (K563) were plated in suspension (density of 0.3 x
25 and 50 µg.mL-1, the positive control (etoposide, 1.25
to 25 µg.mL-1) and negative control (dimethyl sulfoxide)
pernatant discarded. Each cavity received 200µL of MTT
was discarded, and the pellet was resuspended in 150µL
and forward the lineage leukemia (K563) of 29.7 and 17.04
µg.mL-1, respectively. The control reached an IC50 of 4.4
µg.mL-1. Conclusions: We found less cytotoxic action Expenetration of the micelles of the microemulsion-lineage
totoxicity against leukemic strain tested, requiring larger
studies to further conclusions we draw.
Keywords: Schinopsis brasiliensis; microemulsion,
Cytotoxicity (K563).
CPE-1638d
Characterization of patients with chronic diseases in
76
CPE-1639
Prophylactic Effect Of Administration Of The Ascaris Summ Antigen Extracts On Experimental Liver
Injury Mediated Immune Cells
N.T2; Souza, V.M.O1.
1. Department of Immunology, Laboratório de Imunopatologia
Keizo Asami - LIKA/UFPE, Brazil.
2. Department of Pathology, LIKA/UFPE, Brazil.
cells are essential in the pathogenesis of tissue injury, as
Ascaris suum adult worms (Asc) has proteins with regulatory activities that can modulate the immune response to
antigens homologous or heterologous in the host. However, there are few studies of the immunoregulatory action
of this extract in a model of autoimmune hypersensitivity
-
allocated in a control group (which received PBS i.p. and
30 minutes later, PBS i.v.), an AIH (received 300µL of PBS
i.v.). Blood were collected 8, 24 hours and 7 days after administration of treatment to measure transaminases (ALT
of animals through ELISA. Results: Transaminases levels
2011 5 (2)
AST) in relation to AIH+Asc. The total Ig levels were sigmorphometrically quantify the immunostain of galectin-3
not meaningful. After 7 days of administration of ConA,
and invasive ductal carcinoma (IDC) in humans. Immunohistochemistry was developed using monoclonal anti-
in the control group. Conclusions: The results showed that
hepatocellular damage.
Keywords: Ascaris suum; Immunomodulation; Autoimmune Hepatitis.
CPE-1649
Men can become infertile after infection with Chlamydia trachomatis? A focus on semen parameters.
Florencio de Oliveira, T.H.1; Souza Feitosa, A.R.1; Torres
Silva, M.J.1; Oliveira Moraes, R.F.1; Vázquez, C.S.2
1. Faculdade ASCES, Brazil
2. Department of Functional Biology and Physical Anthropology –
University of Valencia, Spain
(DAB) and hydrogen peroxide. Image analysis was carried
out using a workstation consisting of a standard light microscope equipped with a digitalizing camera connected to
a desktop personal computer. Image storage and retrieval
was managed using the OPTIMAS® software system.
Results:The immunohistochemical staining studies dem-
ing to have children, can not generate them, and that the
-
the factors harmful to reproduction is the pathology of in-
Keywords: Immunohistochemistry, tumors, breast
and quality sperm, causing a change in semen parameters,
-
CPE-1682
Synthesis, Structural Characterization and Evaluation
Antinociceptive Activity of the 2 [(benzylidene)-amino] -4,5,6,7-tetra-4H-benzo [b]thiophene-3-carbonitrile
with alterations present in the semen seminal aspects that
serve as a diagnosis for male infertility. Methodology: The
study was conducted through literature review searches
Results: According to the articles analyzed, infection
with Chlamydia trachomatis is the leading cause of epididymitis, and other infections that directly affect the cells
tion of the seminiferous canals, resulting in the call trace
A.R.S,2; Pereira, C.K.S.2; Salvadori, M.G.S.S2; Assis, T.S.2;
Almeida, R.N. 2; Moura, R.O 1; Mendonça-Junior, F.J.B.1.
1. Laboratório de Síntese e Vetorização de Moléculas, Universidade
Estadual da Paraíba, Brazil;
2. Laboratório de Tecnologia Farmacêutica, Universidade Federal
da Paraíba, Brazil
Background: The compounds thiophene derivates, in
great variety of pharmaceutical properties, such as anti-
factor highlighted was the presence of cells involved in
immune response, causing agglutination and changing the
parameters related to motility. Conclusion: Assuming that
synthesis of new thiophene derivative to evaluate their
activity anti-noceptive propriety. Methodology: The syn-
data in relation to semen parameters changed, it is clear
. trachomatis, act directly on male fertility, since these aspects in question are of great importance in the success
of fertilization.
Keywords: Chlamydia trachomatis, infections, infertility
CPE-1650
Immunohistochemistry Detection Of Galectin-3 In
The Breast Tumoral Tissues
Silva, R.B.1; Silva, E.M1; Silva, M.I.C.1; Negreiro, J.S1.;
Morais,J.A.S.1; Silva,J.T.1; Silva, R.C.1; Filho, S.D.M.1;
Patu, V.J.R.M1; Carvalho-Junior, L.B.2.
1. Faculdade ASCES Caruaru- PE,
2. Pós-Graduado em Ciências Biológicas, do Centro de Ciências Biológicas da Universidade Federal de Pernambuco
Background: Immunohistochemistry helps pathologists in
25-35 grams, 3 months of age were divided into groups
with eight animals. The control group received distilled
water and tween 20, 4 experimental groups received the
The compounds were characterized, their structures were
inal constrictions when compared with the control group.
77
Experimental Pathology and Health Sciences
response to the administered dose.
Conclusions:
Was synthesized a new derivative 2-amino thiophene with
Keywords: Thiophene Derivatives, anti-nociceptive,
Gewald reaction
CPE-1683
Behaviour of surgical site infection in splenectomy:
Results of a two-year retrospective study
tos I.T.S1, Xavier A.T1, Oliveira, RAA1; Muniz, MLC1,
Costa, AB1
1. Universidade Federal de Pernambuco-Centro Acadêmico de
Vitória, Brasil
for the mortality of postsurgical patients, where the risk of
sepsis is sixty times higher after a splenectomy procedure.
outside the hospital. Seeing the CAH as a rescuer, he or
cy clinics and in medical and traumatic emergencies which
which occurred during the “Rondon King of the Bay Project” in 2010. Methodology: The training took place in the
city of Cedro-PE, with a total workload of 15 hours of
input, from the 20th to the 23th of July 2010, through
theoretical and practical lessons, using audio-visual material, projections, makeup simulation of complications, speof the undergraduate nursing Victoria Academic Center,
were included in the programe. Results: Previous knowledge of the procedures was scarce and many profession-
years 2009 and 2010. Methodology: This is a retrospec-
results showed that the training was extremely satisfying
and the CHAs demonstrated that they had assimilated the
theoretical content with a good practical performance in
the simulations as well. Conclusions: Training courses for
to 39 patient records. Results: A higher incidence of SSI
-
Keywords: Health education, community health, basic life support.
and mostly hepatosplenic schistosomiasis were operated
CPE-1686
Liver changes in diabetic rats and alcoholics
not perform hair removal. There is no record of preoperathe surgeon. Conclusion: What we try to prove with the
release of our results is that the ISC splenectomies per-
1. Universidade Federal do Vale do São Francisco, Brasil
2. Colegiado de Medicina - Universidade Federal do Vale do São
Francisco, Brasil
3. Colegiado de Ciências Farmacêuticas – Universidade Federal do
Vale do São Francisco, Brasil
Background: Alcoholism is an important inducer of
pathophysiology of liver tissue. These changes are initi-
showing that infection control is done with political decihospital infection.
KEYWORDS: splenectomy, infection; wound infection
CPE-1683a
Initial Care In Urgencies And Medical And Traumatic Emergencies: Training Course Given To Community Health Agents In The Interior Of Pernambuco/
Brazil
Galindo Neto, NM¹; Oliveira, RAA1; Muniz, MLC1, Sanquerque TT1, Silva ACA2, Santos LRC2
1Nursing Department. Federal University of Pernambuco, Vitória
Academic Center, Brazil.
2 Faculdade de Enfermagem Nossa Senhora das Graças-FENSG
Universidade de Pernambuco- UPE
Background: The community health agents (CHA) are a
als and need trainings on different areas. In emergency
situations, the evaluation of the victim and her care should
-
steatosis is not common. Steatosis is the most common
histologic changes, consequent to the excessive deposition
of triglycerides in the liver, occurring most commonly in
alcoholic steatosis, which is more frequent amongalcoholic
liver disease. Methodology: Were used twenty rats (Rattus
concentrations of alcohol solution. The animals had the
were stained with hematoxylin and eosin. Results: Focal
-
Keywords: diabetes; alcoholism; liver.
78
2011 5 (2)
CPE-1686a
Histological analysis of kidneys of diabetic rats alcoholics
1. Universidade Federal do Vale do São Francisco, Brasil
2. Colegiado de Medicina - Universidade Federal do Vale do São
Francisco, Brasil
3. Colegiado de Ciências Farmacêuticas – Universidade Federal do
Vale do São Francisco, Brasil
ure is the second leading cause of death in this disease.
Another factor directly associated with kidney failure is the
divided in groups: untreated (control) and treated with
animals were euthanized and the organs (livers and small
intestines) were collected for histological processing and
posterior morphologic analysis. Results: In the untreated
-
etiologies in pathological processes of renal tissue, this
Methodology: In this experiment, were used twenty rats
application of intra-peritoneal alloxan at a concentration
of FZ-4 was effective in protecting the intestinal mucosa
and the hepatic parenchyma of the granulomatous reaction in mice infected with S. mansoni.
Keywords: FZ-4; Schistosoma mansoni; Morphological analysis.
weeks with increasing concentrations of alcohol solution.
The animals had their right kidney and other organs colpared histological sections stained with hematoxylin and
eosin. Results: At histological analysis, there was extensive
were potentiated in the group alcoholic. Conclusions: Lesions that affect the glomeruli are the most common re-
animals only, the severity was due to enhanced association
of pathologies.
Keywords: diabetes; alcoholism; kidney.
CPE-1707
Effect of FZ4 in Schistosoma mansoni infected mice:
morphological analysis of livers and small intestines
Dias, M.R.P. 1; Bassoli, A.C.D.G.1, Silva, L.L.S.1; Silva,
E.C.1; Pitta, I.R.2; Galdino, S.L.2; Medeiros, P.L.1,2
1. Departament of Histology and Embryology CCB - UFPE,
Brazil.
2. Posgraduate Program in Therapeutic Innovation CCB - UFPE,
Brazil.
Background: According to World Health Organization
(WHO), more than 200 million people in the world are
in situation of infection risk. In Brazil, the illness already
reached the majority of the states, mainly, of the North-
CPE - 1715
Use Of Ulex Europaeus Agglutinin I Conjugated To
Acridinium Ester As A Chemiluminescent Tool For
Of L-Fucose Recognition In Cutaneous Tumours
Bezerra, M.F1, Lima, L.R.A1, Almeida, S.M.V1, Cavalcanti, C. L. B1, Junior, L.B.C1,2, Beltão, E.I.C1,2
1. Laboratório de Imunopatologia Keizo Asami (LIKA), Brazil;
2. Departamento de Bioquímica, Universidade Federal de Pernambuco (UFPE), Brazil.
tions are related to tumour proliferation, metastasis and
evasion of immune system. Lectin histochemistry is a
technique used for the investigation of those glycophenotype changes. Chemiluminescence assay is a promising and
effective technique for quantitative analysis of proteins,
gated to Acridinium Ester (AE) to quantify the L-fucose
maglutinatin activity and chemiluminescence (expressed as
gate, separately, and chemiluminescence assayed. Results:
-
promising tool for quantitative assessment of L-fucose
tivity of conventional histochemistry using haematoxylin
and eosin in skin diagnosis and provides a target for cancer
therapy or diagnosis.
Keywords: chemiluminescence, histochemistry, lectins.
morphology of livers and small intestines of the infected
males, with 45 days of age and weighing of 25-30g had
CPE - 1722
VDRL test in the diagnosis of syphilis. Evaluation of
results of the secretary of health Sapé – PB
-
this disease. In searching for molecules with antiparasitic
79
Experimental Pathology and Health Sciences
1. College of Santa Emilia Rodat, Brazil.
2. State University of Paraiba, Brazil.
Introduction: Syphilis is a systemic infectious disease with
clinical and pathological manifestations. The diagnosis is
tives: This study aims to analyze the results of tests non
tory) of patients seen in primary care unit. Methodology:
lous district of the city. The most frequent clinical manifestations were keratoconjunctivitis and onychogryphosis
demonstrated an increase in the cases of CVL in the period and the most endemic area was concentrated in the
medium and lower-medium social level district. The clinical analysis in infected dogs showed that the most frequent
Keywords: anthropozoonosis; leishmaniasis, urban
reservoir
uses the extracted tissue antigens such as cardiolipin, a liplecithin and cholesterol, form a serologically active antigen
disease, can occur so false-positive results due to disease
tions among others. (VAZ et al.1996; LIMA et al.1999,
-
results demonstrate the presence of syphilis in our day and
the importance of this test especially during pregnancy, in
order to avoid contamination placenta. Conclusion: Al-
CPE - 1723
Analysis of the healing time of skin wound on mice
submitted to treatment with plant extracts
Pereira, M.N.1; Guimarães B.F.1; Longatti, T.R. 1; Oliveira,
C.D. 3; Moreira, G.A. 1; Oliveira, R.J.4, Santos, H.B. 2; Ri1. Laboratório de Biologia Celular e Mutagênese - LabCcel/CCO/
UFSJ, Brazil
2. Laboratório de Processamento de Tecidos - LAPROTEC/
CCO/UFSJ, Brazil
3. Campus Centro Oeste - Universidade Federal de São João Del
Rei, Brazil
4. Molecular Oncology Research - Barretos Cancer Hospital, Brazil.
peutic value is associated with many cultures, especially in
the medicinal effectiveness or the toxicity of analyzed ex-
with clinical assessment, early diagnosis, and therapeutic
Keywords: Syphilis, VDRL
CPE – 1722a
Clinical and epidemiological study of dogs with Visceral Leishmaniasis in the city of João Pessoa, Paraíba, Brazil
1. State University of Paraiba, Brazil.
2. Zoonoses Control Centre, João Pessoa. Brasil
Introduction: The Canine Visceral Leishmaniasis (CVL) is
serious systemic zoonoses with chronic and slow progress
agent is the protozoan Leishmania (Leishmania) chagasi,
link in the transmission cycle of CVL, through of the Luttreated at Zoonoses Control Center in the city of João Pessoa-PB and to identify the most common clinical alterations in the symptomatic animals. Methods: The data were
the analysis of the time of healing in injuries induced and
treated with vegetal extracts. Methodology. The Plantago
topical treatment was with watery solution of two extracts
(G2 and G3), controlled group (water) and as positive control, an ointment especially used in postoperative wounds.
uted in 4 treatments with 14 animals each. Closing wounds
time and injuries appearance were evaluated during 21
days. Results. The control group (water) and G2 presented
healing times smaller. The group treated with the ointment
presented the worst performance. Macroscopically, it was
10th and 15th day after injuries. Moreover, all the animals
of these two groups had completed the healing of the
Plantago (G2) presented minor time of healing in relation
in the period of 2005 to 2009. Twelve dogs seropositive
the clinical characteristics analyzed. The technique applied
statistical difference when compared to the group treated
with the ointment.
Keywords: Wound healing. Extracts of plants. Mice
CPE - 1725
Development of an electrochemical biosensor based
on gold nanoparticle-polyaniline composite for tuberculosis detection
Maurilia Palmeira da Costa1, César A. S. de Andrade1,
cases). The data suggest that the most endemic districts
were in the south area, with Bairro dos Bancários, Valen80
1- Centro Acadêmico de Vitória, UFPE, Brasil; 2- Centro de Pesquisas Ageu Magalhães, Recife, Brasil; 3- Departamento de Bio-
2011 5 (2)
química, Centro de Ciências Biológicas, UFPE, Brasil.
CPE - 1726
Detection of viable Mycobacterium leprae in water
samples from Juazeiro do Norte and Crato, Northeast Brazil
Holanda, M.V.¹; Oliveira, T. E. S.¹; Macedo, M. L. B.¹;
The gold nanoparticles (AuNps) facilitate the transfer of
sis (MTB) under the surface of gold electrode (Au) to analyze their interaction of AuPANi-MTB system and DNA
spectroscopy (EIS) was performed in a frequency range of
100 KHz to 100MHz and cyclic voltammetry (CV) with
a voltage of -0.2 to 0.7 V, in the presence of potassium
ferricyanide 10mM as redox couple. The EIS and CV measurements demonstrated decreased the redox reactions of
MTB system demonstrated an additional decrease in real
1. Laboratory of Mycobacteria, School of Medicine, Federal University of Ceará, Brazil
2. Department of Geology, Federal University of Ceará, Brazil
Background: Leprosy is a chronic dermatological and
tal water samples in endemic areas of Juazeiro do Norte
and Crato, Northeast Brazil. Methodology: Water samples
from rivers, lakes and damns were collected in sterile dark
with AuPANI-MTB and MTB-AuPANI-TB2, a decrease
-
in total 45 water samples. The water samples were processed for total RNA extraction using PowerWater RNA
Isolation kit (MoBio). cDNA was synthesized using the
OneStep RT-PCR kit (Qiagen) and primers targeting gyrA
Keywords; biosensor, tuberculosis, impedance, bioelectrochemistry
CPE – 1725a
Development of an electrochemical biosensor based
on gold nanoparticle-polyaniline composite for tuberculosis detection
Costa,M P1; Andrade, C A S1; Melo F L2; Oliveira, M D
L3
1- Centro Acadêmico de Vitória, UFPE, Brasil; 2- Centro de Pesquisas Ageu Magalhães, Recife, Brasil; 3- Departamento de Bioquímica, Centro de Ciências Biológicas, UFPE, Brasil.
ervoir all samples were positive. Conclusion: The recovery
of RNA sequences from an RNA template using RT-PCR
implies that the source organisms were live at the time of
ing a role in the continued transmission of the disease.
Keywords: environmental water; Mycobacterium leprae; viable bacilli
CPE - 1727
The gold nanoparticles (AuNps) facilitate the transfer of
sis (MTB) under the surface of gold electrode (Au) to analyze their interaction of AuPANi-MTB system and DNA
spectroscopy (EIS) was performed in a frequency range of
100 KHz to 100MHz and cyclic voltammetry (CV) with
a voltage of -0.2 to 0.7 V, in the presence of potassium
ferricyanide 10mM as redox couple. The EIS and CV measurements demonstrated decreased the redox reactions of
MTB system demonstrated an additional decrease in real
abattoirs at Pernambuco Zona da Mata Norte
Pereira, M.F.1
1- Departament of Veterinary Medicina of Federal Rural University of Pernambuco, Brazil
that, in some way, prevent or constrain the use of the product or raw material for human consumption. Post-mortem
human consumption, verifying the presence of changes in
organs and tissues that may indicate the existence of dis-
with AuPANI-MTB and MTB-AuPANI-TB2, a decrease
photographed and fragments were collected and placed in
Keywords; biosensor, tuberculosis, impedance, bioelectrochemistry
81
Experimental Pathology and Health Sciences
and stained with hematoxylin and eosin (HE). Results: Approximately 60 animals were slaughtered (30 at Carpina
animals. Among them, there are eight with swelling of
clusions: In slaughterhouses visited, post-mortem changes
were mainly found in lymph nodes and lungs.
Keywords: Inspection, pathology, cattle.
CPE – 1728a
volved different genetic, immunological and environmenfrom the Hospital das clínicas (HC) and the Barao de
Lucena Hospital (HBL) with factors such as gender, age,
were selected. The inclusion criteria for CD patients were
tion criteria of Lennard-Jones (1989). All patients were
Oral Ulcers Of Rats Treated With Bixa Orellana L.
Extract (Annatto, 5,5% Bixin)
Souza, M.F.A.M.1. ; Santos, J.A.A.1. ; Silva, E.L.V.1. ; Aguiar Jr, F.C.A.1.
1. Laboratory of Biotechnology and Pharmaceuticals, Academic
Center of Vitoria – Federal University of Pernambuco, Brazil
Background: The healing of oral ulcers is a complex
cal events. Mast cells are connective tissue cells that act
host defense and homeostasis. Annatto is an important
-
-
reveal that, in the study area, the CD is more common in
men, working-age adults, mixed ethnicity, and presented
the shape of stenotic disease.
Keywords: Crohn’s disease, epidemiology.
cally induced in rats treated with of Bixa Orellana L extract
CPE - 1732
Evaluation
Of
The
Inclusion
Complex
Of
Models
Laranjeira, L.P.M 1; Sitônio, M. M 1; Carvalho, C. R 1;
cm diameter wounds were surgically made on the dorsal
tongue surface of thirty male wistar rats. The tongue ulrats on the 6th hour, and 1st, 3th, 7th and 14th postoperative days were processed for histology. The histological
A.J.S1; Silva, T.G. 1.
1. Laboratório de Bioensaios para Pesquisa de Fármacos, Departamento de Antibióticos - Universidade Federal de Pernambuco, Brazil
2. Laboratório Tecnologia de Medicamentos, Departamento de
Farmácia - Universidade Federal de Pernambuco, Brazil
Background: There are in the market several drugs for
mon in pharmacotherapy is the use of NSAIDs, which
mast cells in the treated animals was reduced on the1st
lapachol, which possess therapeutic properties and toximast cells during the healing process of oral ulcers in rats.
Keywords: Mast cells; tongue; Annatto.
CPE - 1730
Epidemiological aspects of Crohn’s Disease in Hospitals from Pernambuco, Brazil
Santos, L. P.1; Fernandes, W. N.2; Telles, A. M. S.4; Lima,
F.E.B.A.5; Martinelli, V. 5; Toscano, M.6; Melo-Junior,
M.R.3,4
1. Centro de Ciências da Saúde - UFPE, Brazil.
2. Programa de Pós-graduação em Patologia - UFPE, Brazil.
3. Laboratory of Immunopathology Keizo Asami - LIKA/
UFPE, Brazil.
4. Department of Pathology - UFPE, Brazil.
5. Hospital das Clínicas- UFPE, Brazil.
6. Serviço de coloproctologia do Hospital Barão de Lucena, Brazil.
heterogeneous group of diseases wich the last common
82
realized paw edema carrageenan-induced and rheumatoid
To edema formation in rats, a 0,1mL carrageenan soluthe right hind paw. The animals were pre-treated (p.o.) 60
measured with a plethysmometer immediately prior to the
injection of carrageenan and thereafter at hourly intervals for 5 h. In the arthritis induction, the treatment was
On the third day, 0.1 µL FCA was injected in the right hind
(mL) during 14 days, using plethysmometer. Results: The
the fourth hour (0.35±0.07), when compared with saline
control (0.50±0.09). In the rheumatoid arthritis develop-
2011 5 (2)
tion, when compared with the control (1.00±0.03). Con-
ated from nested two-stage PCR of RLEP region and a
rRNA primers respectively. Discussion: The most sensimatory
repeated 28 times in the M. leprae genome. The primers
CPE - 1733
less sensitive. In addition, nested PCR increases the sen-
Leprosy in Fortaleza-Ce
Assis, LC 1; Lima, LNGC 1; Marques, LCM 1; Dantas, JCP
1; Kerr, LRS 1; Frota CC¹.
Laboratory of Mycobacteria, Medicine School, Federal University
of Ceará, Brazil
Introduction: Single nucleotide polymorphisms (SNPs)
of DNA from clinical samples. However, reactions using a
hinder the interpretation of agarose gel. Conclusion: The
of M. leprae from clinical samples with small amount of
Keywords: Leprosy, PCR, molecular detection
leprae prevalent genotypes in patients with leprosy in Fortaleza-Ceará Methodology: Nasal samples from 34 leprosy
teinase K. The SNPs were divided into two groups, called
genotypes 1 or 2 and 3 or 4. Genotyping was performed
CPE - 1735
In silico analysis of the most expressed 14 contigs in
a Leishmania chagasi cDNA library from the Northeast Genome Program
Lins Marinho, L.A.¹; Oliveira, E.B.¹; Zacarias da Silva,
Silva, E.C.¹
1 – Tissue Culture Lab/Department of Histology and Embryology/CCB-UFPE, Brazil
2 – Genetics Department/CCB - UFPE, Brazil
3 – University Salgado de Oliveira – UNIVERSO, Brazil
Background: Visceral leishmaniasis or kala-azar is a endemic zoonosis in the Americas, having Leishmania chagasi as its etiological agent. Its genome comprises ca. 8,500
genes spread among 35 chromosomes. The Northeast
transcriptome of this protozoan. The consortium pro-
all samples were male. A similar rate of male and female
was seen among the samples that did not amplify. On the
other hand, three male had nasal samples with different
genotypes. Conclusion: The genotype 1 or 2 of M. leprae
was the most prevalent in our study population. Genotype
lent in Ethiopia.
Keywords: Genotyping, SNP, Mycobacterium leprae.
CPE – 1733a
Comparison of 16S rRNA and RLEP genomic releprae from nasal clinical samples
Assis, LC 1; Marques, LCM 1; Lima, LNGC 1; Dantas, JCP
1; Kerr, LRS 1; Frota CC¹.
Laboratory of Mycobacteria, Medicine School, Federal University
of Ceará, Brazil
petitive element) and 16S rRNA regions are used for
molecular detection of M. leprae DNA. OBJECTIVE:
To analyze the PCR positivity of the regions 16S rRNA
and RLEP in the diagnosis of M. leprae from nasal cliniquence; while samples from 169 patients were analyzed
-
most expressed contigs, depicting their functions and
chromosome position. Methodology: Sequences from
ac.uk), using the online tools BLASTn e BLASTx. Putative
sults: From the 14 contigs studied, one mapped on chromosome 8, another on chromosome 23 and all other on
proteins from Leishmania and the other 12 were similar to
presence of rRNAs among the most frequent ESTs is exnot have as assigned function until now. This result opens
lism and pathogenicity.
Keywords: Leishmania; cDNA library; Genome
CPE – 1750
Pernambuco in during the period 2009 to 2011
83
Experimental Pathology and Health Sciences
BGS4; Medeiros, JH5; Lorena Júnior, EJP6; Santos, LS7;
of the holder, also makes it mister disclosure of Pap smears
1,2,3,4,5,6,7,8,9 e 10.Department of Nursing. Federal University
of Pernambuco, Brazil.
disease.
Introduction: The exogenous intoxication is the manifes- Keywords: papillomavirus, neoplasms, cytology
tation, through signs and symptoms of the harmful effects
produced in an organism, a result of interaction with one CPE - 1753
Cytopathology Changes In Women That Made Use Of
- Intrauterine Device (Biomaterial)
- Ordonho, Kamyla Brasileiro De Lima.¹ ; Araujo, Thereza
Cristhinna.¹; Travassos, Karine Dantas.1; Oliveira, Stepha1 - Academic Biomedicine Maurice of Nassau - Brazil
2 - Lecturer in Biomedicine Maurice of Nassau - Brazil
-
-
circumstances, and evolution of intoxication. Results:
During this period 10,385 cases were reported, predomi- inexpensive changes in the diagnosis of morpho-cells in the
without sequelae. Conclusion: These indexes suggest the
promotion of control measures and health education focusing on the group most at risk, as well as reducing the
incidence of self-annihilation.
Keywords: exogenous poisoning. Public health. Suicide
an exploratory study of quantitative approach to document
the data collection was performed in the compulsory period
of Campina Grande - PB. Results: The results were as follows. Of the 48 cytological study showed that the use of
CPE - 1752
Analysis Of The Prevalence Of Human Papillomavi- health policies directed to this area particularly with the presrus Women In The City Of Esperança – Pb
ence of family health programs and monitoring of patients
Travassos, Karine Dantas1; Oliveira, Stéphanny Sallomé
Dias1; Araújo, Thereza Cristhinna1; Sousa, Giovanni Tavares2.
1 – Academic of Biomedicine Maurice of Nassau – Brazil
2 - Student of Biomedicine Maurice of Nassau – Brazil
Introduction: The Human Papillomavirus is now considered as one of the diseases that most affect women world-
Keywords: IUD, Papanicolaou smear results
CPE – 1755
Prevalence of intraepithelial high-grade injuries in
the rectum and anal canal in patients with a history of
squamous neoplasia of the lower genital tract
it has strong link with cervical cancer and is one of the R.I.M.A. 1
- 1. Universidade Federal de São João Del Rei. CCO/UFSJ, Brasil
of cervical cancer has a strong association with the occurprevalence of women aged 20 to 50 years with results of rence of anal canal cancer. Its early detection allows the preology: To this end we conducted a research of descrip- virus (HPV) anal infection in patients with cervical intrative type with a quantitative approach with a retrospective epithelial neoplasia.
documentary. Included were women aged 20 to 50 who
had Pap smears, as soon was used as the research instru- four women with cervical intra-epithelial neoplasia, treated
surgically or not, without chemotherapy or radiotherapy
health department of the city of Esperança - PB (1116
laou. Koilocytosis indication was considered pathognomonic of HPV infection and lesions indicative of anal intraepithelial neoplasia grade I.
Results:The average age of patients was 36 years, and aver67 patients had at least CIN I, the highest prevalence was
of patients stated they do or have already made use of oral
contraceptives. These aspects are risk factors for infection
respectively. Conclusions: Considering the results empha- canal HPV infection and AIN grade 1: they have Koilocytosize the importance of preventive measures adopted and
84
2011 5 (2)
not found cellular changes.
Conclusions:The high percentage of the sample with anal
sions of cervical cancer population and precursor lesions
anal cancer population. It means that need for Pap screen-
the transmission usually occurred when dogs eliminate oo-
cervical cancer, allowing early diagnosis of anal cancer preA total of 59 sera samples from dogs with no neurological
Keywords: Intraephitelial injuries, Cervical intraepithelial neoplasia, anal canal
off point. Results: The frequency of positive samples was
CPE - 1760
Etiology Survey of SubcCinical Mastitis (SCM) in
Brazil
Bezerra, M. J. G.¹; Silva, L. B. G.4; Mota, R. A.4
1. Discente do Programa de Pós – Graduação em Ciência Veterinária da Universidade Federal Rural de Pernambuco (UFRPE),
Brasil;
2. Discente de graduação em Medicina Veterinária da UFRPE,
Brasil;
3. Discente do Programa de Pós – Graduação em Biociência Animal
da UFRPE, Brasil;
4. Professor Associado do Departamento de Medicina Veterinária
da UFRPE, Brasil
of positive samples were reagents in the titration 1:50 and
1:60 with no associated clinical disease signs. Conclusions:
The Neospora caninum infection is present in dog population examined and the male dogs showed higher positivity
than females.
Keywords: neosporosis, diagnosis, canines
CPE – 1760b
Presence of Ureaplasma sp. in semen samples of
sheep from the State of Pernambuco, Brazil
Silva, J. G.1; Santos. A. S.1; Kim. P. C. P.1; Santos, S. B.1;
Mota, R. A.1
-
effects on production, composition, quality of milk and
genital tract, and that can affect the reproductive perfor-
Methodology. Milk samples were collected from 52 goats
Methodology: A total 50 sheep of reproductive age and
-
The DNA was extracted from the semen samples using a
tions. The PCR mixture (17,25 µL) contained 5µL of template DNA; 0,5 µL primers (30pmol each); 5 µL of water
and 6,25µL of MasterMix commercial kit, according to the
and Bastonete of gram-negative associated with mastitis.
spp. were more common pathogens associated with in
mastitis in this study. These data indicate the importance
of preventive hygienic of milk utensils and teat manageKeywords: Microbiology, Milk Goat, Mammary gland
CPE – 1760a
Antibodies research anti-Neospora Caninum in dogs
diagnosed in the Veterinary Hospital of UFRPE, Recife, Brazil
Freitas, R. S. V.1; Machado, A. C.2; Silva, J. G.1; Souza
Neto, O. L.1; Alves, B. H. L. S.1; Melo, R. P. B.1; Santos, A.
Mota, R. A.4
1. Discente de graduação em Medicina Veterinária da UFRPE,
Brazil;
2. Discente do Programa de Pós – Graduação em Ciência Veterinária da Universidade Federal Rural de Pernambuco (UFRPE),
Brazil
3. Discente do Programa de Pós – Doutorado da UFRPE, Brazil;
4. Professor Associado do Departamento de Medicina Veterinária
da UFRPE, Brazil
Background: Neosporosis is a parasitic disease character-
animals in the region.
Keywords: Ureaplasma; semen; sheep herds
CPE – 1764a
Healthcare Nursing to the patient with pancreatic
tumor that was submitted to gastroduodenopancreatectomy
Medeiros, J.H.1; Silva, D.C.A.1; Silva, D.M.1; Silva,
ra, J.M.D.1; Santana, P.R.1
1. Federal University of Pernambuco, Brazil
Introducion: the tumor of the pancreas has high mortaland lack of aggressiveness of the treatment really effecquestion had their diagnosis carried out after indicate lo85
Experimental Pathology and Health Sciences
calized pains in the region and complain of tumefaction,
CPE – 1765a
-
agulase Negative Staphylococcus Isolated Of Diverse
Regions Of Health Care Professional Of Hospital
Universitary
rier of the same framing a SAE as regards post-operative.
Metodology: Held at the surgical clinic North Wing of the
1. Bacteriology Laboratory / Department Of Tropical Medicine University Federal De Pernambuco, Brazil
Introduction: The Staphylococcus coagulase negative
skin and, although they were considered saprophytes for
nesis and physical examination of the patient, information
achieved with their families and health record data, mak.Resultads: The appropriate care for a patient after surgery
are essential for an adequate recovery, avoiding infections
or any worsening in the clinical picture. For this to need
they prevail in numerous organic situations to cause serious infections. There are currently related to numerous
nosocomial infections, and the main vehicle of transmission are the hands of health care professional (HCP), who
SCN isolated from various regions (nasopharynx, hands
and cell phones) of HCP. Methods: The Staphylococcus
SAE post surgical assistance for the recovery of the client.
Keywords: Cancer, SAE, Care
samples of HCP (doctors, nurses and nursing assistants),
CPE - 1765
agulase Negative Staphylococcus Isolated Of Diverse
Regions Of Health Care Professional Of Hospital
Universitary
1. Bacteriology Laboratory / Department Of Tropical Medicine University Federal De Pernambuco, Brazil
Introduction: The Staphylococcus coagulase negative
using discs of penicillin (PEN), oxacillin (OXA), cefoxitin
(CFO), vancomycin (VAN), gentamicin (GEN), erythromycin (ERI), clindamycin (CLI), chloramphenicol (CLO),
to CIP. Conclusions: The results of this study support the
literature, showing a higher incidence of resistance pro-
skin and, although they were considered saprophytes for
they prevail in numerous organic situations to cause serious infections. There are currently related to numerous
nosocomial infections, and the main vehicle of transmission are the hands of health care professional (HCP), who
SCN isolated from various regions (nasopharynx, hands
and cell phones) of HCP. Methods: The Staphylococcus
samples of HCP (doctors, nurses and nursing assistants),
using discs of penicillin (PEN), oxacillin (OXA), cefoxitin
(CFO), vancomycin (VAN), gentamicin (GEN), erythromycin (ERI), clindamycin (CLI), chloramphenicol (CLO),
to CIP. Conclusions: The results of this study support the
literature, showing a higher incidence of resistance pro-
for cell phone and hands of the them in order to avoid
contamination of the same or patients.
agulase negative health professionals
86
for cell phone and hands of the them in order to avoid
contamination of the same or patients.
agulase negative health professionals
CPE - 1789
Acceptability of food made from yam (Dioscorea sp.)
by students of a private school in the city from João
Pessoa, PB
De Lima, K.N.1; De Sousa, T.A.2; Santos, J.S.2; Oliveira,
S.M.S.1
2. Faculdade Santa Emília de Rodat- Biomedicina, Brazil
Introduction: In the Northeast, the yam is a source of
nutrients and income for small and medium farmers, as
part of food, mainly rural population, possessing a variety of nutritious and tasty dishes. The development of
drates, proteins, vitamins and minerals and low in fat, is
a good choice of snack for children of school age. The
school is where children spend a period of time every day,
making it an important site for the formation of eating
important it is that the school canteens sell nutritious
jectives: This study aims at encouraging the consumption
of yam in populations of school children, aged 7 and 12
years, through culinary innovations, such as yam pie, so as
2011 5 (2)
to achieve a healthier diet and alternative and verify the
evaluation of the sensory parameters (taste, odor, texture
CPE – 1790a
Eritrócitos bovinos como modelo experimental para
o uso de manitol no combate a edemas: teste comparativo com outras moléculas
and a hedonic scale. Results: According to the results of
pie of yam in the school meals. The results of the facial
sults it was concluded that the preparations with yam had
T.C.M.3; Carvalho, M.M.S.G.2; Santos, E.P.
1. Federal University of Paraíba, Brazil;
2. State University of Paraiba, Brazil;
3. Faculdade Santa Emilia Rodat, Brazil
Bovine erythrocytes as an experimental model for the use
other molecules
Background: Given the importance of understanding the
mechanisms of maintenance of the osmolarity for stu-
of nutrition.
Keywords: Acceptability; yam; students
CPE - 1790
Evaluation cytotoxic against Artemia Salina and antinociceptive activity of 2-amino-4,5,6,7-tetrahydrobenzo [b] thiophene-3-carbonitrile derivative
A.R.S,2; Pereira, C.K.S.2; Salgado, P.R.R.S. 2; Assis, T.S.2;
Almeida, R.N. 2; Moura, R.O 1; Mendonça-Junior, F.J.B.1*.
1. Laboratório de Síntese e Vetorização de Moléculas, Universidade
Estadual da Paraíba, João Pessoa 58020-540, Brazil;
2. Laboratório de Tecnologia Farmacêutica, Universidade Federal
da Paraíba, Caixa Postal, 5009, João Pessoa 58051-970, Brazil
Background: Thiophenes derivatives are endowed with
anti-fungical and other. However, it is important to know
its cytotoxicity. An alternative to conventional cytotoxicity
tests are the tests with the microcrustacean Artemia salina,
passively altering the intracellular concentration and, consequently, the results of the experiment. The rule extends
to tissue and systemic level. According to some studies,
controversy regarding the use, for example, in countering
the effects of acute stroke to reduce intracranial pressure,
promoting an increase in osmotic pressure of the vessels,
ciency of the osmotic mannitol as compared with other
molecules in order to demonstrate the effect of tone on
lar solutions (glucose, mannitol, urea) and ionic (KCl and
tion:
assesses the antinociceptive and cytotoxic activity of a
varying the speed of water entering or leaving the cell.
Conclusions. In experimental terms, the molecular nature
separated ten Artemia salina newly hatched and added to
and 4.5 mL of synthetic saline water. The solution was
diluted in the following concentrations standard (1000 mg
acid was performed using Swiss male mice, 25-35 grams,
3 months of age, divided into groups with eight animals.
The control group received distilled water and tween 20, 4
sults: The thiophene derivative showed high cytotoxicity
the molecular level.
Keywords: Edema; osmolarity, mannitol
CPE – 1793
Histopathologic Evaluation Of Skin Wound Healing
Treated With Bixa Orellana L. Extract (Annatto, 2,5%
Norbixin) In Rats
Santos, J.A.A.1. ; Souza, M.F.A.M.1. ; Silva, E.L.V.1. ; Aguiar Jr, F.C.A.1.
1. Laboratory of Biotechnology and Pharmaceuticals, Academic
Center of Vitoria – Federal University of Pernambuco, Brazil
Background: Annatto is a red-yellow dye extracted from
the mature and dry seed pericarp of Bixa orellana L. (Bixa-
tractions, results very close to those found in morphine.
Conclusion: The compound tested showed high cytotoxic-
-
however the results antinociceptive were considered mean
Key-Words: Thiophene Derivatives, Anti-nociceptive,
Citotoxity activity
effective for quenching free radicals and act as a potential
antioxidant. As annatto is large used as a coloring material,
-
87
Experimental Pathology and Health Sciences
were investigated in surgically-induced skin wounds of rats
thelialization and angiogenesis during skin wound healing
process in rats.
Keywords: wound; alpha-pinene; rats
thickness skin wounds were surgically performed on the
21st postoperative days were processed for routine hisnewly formed capillaries, and collagen deposition. For stare-epithelization on the 1st, 3rd, 7th, 14th and 21st days
postoperative. Conclusions: The topical use of the annatto
Keywords: Bixa orellana L.; Wound healing; Rats
CPE - 1795
Prospects of using stem cells in Alzheimer’s disease
Firmino, J.M.1; Diniz, D.A.M.1; Benício, T.C.M.1; Alves,
M.A.S.5; Santos, J.C.S.6; Santos, E.P.1; Souza, J.P.1,4; Carvalho, M.M.S.G.6,7
1. Santa Emilia de Rodat Faculty, student of Biomedicine, Brazil
2. Federal University of Paraiba, student of Technical Biodiagnostic, Brazil.
3. Federal University of Paraiba, student of Nursing, Brazil.
4. Federal University of Paraiba, student of Química, Brazil.
5. Unida da Paraíba Faculty, student of in nutrition, Brazil.
6. State University of Paraiba, Brazil
7. Oral Biology Laboratory, Health Science Center, Federal University of Paraiba, Brazil
form of dementia among the elderly, leading to severe
and genetic risk factors, acquired and environmental hypotheses are reported as causes for the development of
CEP – 1793a
Biological Effects Of Topical Treatment With AlphaPinene In The Healing Of Skin Wounds In Rats
Santos, J.A.A.1. ; Cavalcanti, C.C.L. 2. ; Souza, M.F.A.M.1.
; Cavalcanti, T.R.L.2.; Lima, S.M. 2. ; Araújo, R.M.4.;
Ximenes, R.M.3.; Martins, R.D.2.; Aguiar Jr, F.C.A.1.
1. Laboratory of Biotechnology and Pharmaceuticals, Academic
Center of Vitoria – Federal University of Pernambuco, Brazil.
2. Laboratory of Physiology and Pharmacology, Academic Center
of Vitoria – Federal University of Pernambuco, Brazil.
3. Departament of Physiology and Pharmacology – Federal University of Ceará, Brazil.
4. Federal University of Rio Grande do Norte, Brazil.
standing of AD and the prospects for treatment of the
same through the therapeutic use of stem cells. Methodology: It consisted of a literature review using the key
nal cortex, hippocampus and neocortex. With the discov-
species of conifers. This compound is one of the chemo-
from stem cells which, in turn, derived from primordial
urundeuva popularly known as “aroeira-do-sertão”. Several medical agents used in wound care are derived from
-
pinene has reported several pharmacological actions, such
treatment with alpha-pinene in surgically-induced skin
wounds in an animal model. Methodology: A full-thickness dermal excision wound was created on the shaved
dorsum of third-six male wistar rats. The animals were
randomly separated in three groups with 12 rats each:
of life of patients with this disease.
Keywords: Alzheimer’s disease, stem cell, neurogenesis
closure was monitored using the wound-healing microand newly formed capillaries. For statistical analysis, the
CPE - 1807
Repellent effect of Indigofera suffruticosa (Fabaceae)
on Aedes aegypti (Diptera: Culicidae) reproduction
Lima, I.R. 1 ; Vieira, J.R.C. 1; Leite, S.P. 1
1. Departamento de Histologia e Embriologia- CCB/UFPE, Brazil
Background: Indigofera suffruticosa is a plant of the Fa-
2 on the 14th day after surgery. Re-epithelialization was
accelerated in groups 1 and 2 on 7th postoperative day
commonly known as an IGR) are products or materials
treated animals from group 1 and 2 on 7th postoperative
-
surfaces which discourages insects (and arthropods in
88
2011 5 (2)
quency sounds). The mosquito Aedes aegypti is a vector
promotes the dissemination of four serotypes of dengue
purpose of this study was to investigate the deterrent activity on oviposition of A. aegypti treated aqueous extract
from leaves of I. suffruticosa. Materials and Methods:
During 4 consecutive days, 90 female mosquitoes of A.
aegypti were stored in polypropylene cages (30 × 30 × 30
cm) (Bugdorm-I, Mega View Science Education Services,
Campos, I.A.1, Carvalho, C.R. 1, Laranjeira, L.P.M. 1,
Sitônio, M.M. 1, Silva, J.B.N.F. 1, Mota, F.V.B. 1, Mesquita,
A.R.C. 2, Ximenes, E.C.P.A. 2, Silva, T.G. 1
1. Laboratório de Bioensaios para Pesquisa de Fármacos – Departamento de Antibióticos/UFPE, Brazil
2. Laboratório de Bioquímica e Fisiologia de Microorganismos, Departamento de Antibióticos/UFPE, Brazil
associated with a failure in the immune system host reties against pyogenics cocci and enteric pathogenic yeast.
3.5). Results: In the deterrent activity the aqueous extract
the posture of eggs of A. aegypti (88 (13–210)), compared
with the control in distilled water (343 (185–406)). Conclusion: This research demonstrated that the species I. suffruin the medical and pharmaceutical industries.
Keywords: Aedes aegypti; Indigofera suffruticosa; repelent
CPE – 1807a
Behavioral evaluation in mice bearing sarcoma 180
treated with leaves of Indigofera suffruticosa
Lima, I.R.1; Vieira, J.R.C. 1; Leite, S.P. 1
1. Departamento de Histologia e Embriologia- CCB/UFPE, Brasil
Background: Indigofera suffruticosa is a plant of the
aqueous extract and Indican isolated from I. suffruticosa
showed that plant has antitumor activity. Behavioral responses in mice are important tools to detect toxic effects
the development of sepsis on animal models. Methodology: A total of 36 mice Mus musculus were divided into 3
groups. The animals received administrations (p.o) twice a
gation and puncture (CLP) was performed on the 11º day.
The sham group underwent the surgical procedure without induction of sepsis. Six animals of each group were
neal cavity washed with PBS+EDTA (3 mM). Half of the
animals from each group were left to assess the survival
rate. The leucogram was realized with hematologic anascience). The statistical analysis was made using ANOVA
increase of survival rate and increased the leukocytes migration (6.32±0.47) comparing with groups (4.40±0.60)
ZM (66.45±2.60) comparing with control (57.26±2.68)
and sham (20.86 ±0.47). IL-10 levels increased in ZM
(2574.04±81.12) comparing with control (1328.48±6.29)
and sham (52.95±1.54). Conclusion: In agreement with
with the aqueous extract and Indican from leaves of I. sufmusculus), male, young adults, 60 days old, weighing from
intraperitonially). It was injected into the right axillary 0.3
mL (around 3x106 cells) of the ascitic form of Sarcoma
minutes to 4 hours after administration of the extract and
cervical dislocation. Results: After the end of the toxicoaqueous extract and Indican from leaves of I. suffruticosa
showed some stimulant and depressor actions in mice
sion: It follows that the aqueous extract and the Indican
from leaves of I. suffruticosa showed some alteration of
Nervous System.
Keywords: Indigofera suffruticosa; sarcoma 180; animal behavior.
CPE – 1808
Zymomonas mobilis improves survival rate in CLPinduced lethal sepsis in mice
against pathogenic microorganisms.
Keywords: Zymomonas mobilis; probiotics; sepsis
CPE - 1810
Histological changes of the adrenal gland of pinealectomized female rats
Sousa, I.D.D.¹; Dias, S.M.S.¹; Santos, K. R. P.¹
1. Laboratório de Biotecnologia e Fármaco - UFPE/CAV, Brazil
there are still divergences in these results, since there is
no consensus on which cortical layer suffers such changes.
tological changes the adrenal gland of 45-day-old pinealectomized rats. Methodology: We used 20 female rats of
Wistar lineage. The animals were divided in two groups:
pinealectomized rats and sham-pinealectomized rats (control). At the end of the experiment (7 months after surgery), the animals were anesthetized and euthanized, the
mitted to the hematoxylin-eosin staining and examined
under light microscopy. Results: The pinealectomized female rats showed increased vascularization of all cortical
layers. Was detected a larger development of the zona fas89
Experimental Pathology and Health Sciences
to the same cortical zone in the sham-pinealectomized
Co. Ltda. Despite its increasing use, its side effects should
the pinealectomy, provokes changes in the histological
structure of the adrenal gland in rats, specially in the zona
reports of sexual dysfunction in erection, ejaculation and
effects of treatment with olanzapine in mice Mus muscu-
physiopathology.
Key-words: Pinealectomy; Adrenal glands; histology.
CPE – 1811
Action of the fractions of species of the genus Vernonia in the inhibition of gelatinase A
Moreira, G.A¹; Matos, L.G.¹; Longatti, T.R. ; Oliveira,
1. Laboratório de Biologia Celular e Mutagênese - LabCcel/CCO/
UFSJ, Brazil
2. Molecular Oncology Research - Barretos Cancer Hospital, Brazil.
ases (MMPs). These enzymes are known to degrading extracellular matrix components, and MMP-9 (gelatinase A)
Vernonia in the action of the gelatinase A. Methodology:
Vernonia: hydroalcohol, hexane, chloroform and ethyl acsitometry was performed using Scion Image. Phytochemi-
morphometric analysis of this organ. Methodology: We
ceived deionized water while the treatment groups were
col methacrylate (Leica). Histological sections of 4 mm
analyzed morphologically and morphometrically. Results:
ences regarding the height of the villus epithelium of the
seminal vesicles, when compared the control group and 20
mg treated group, where you realize that there was a reduction in seminal vesicle epithelium in the group treated
with 20 mg olanzapine in the control group. Conclusion:
the relevant parameter analyzed the seminal vesicle, this
relevant information for patients who are dependent on
their use.
Keywords: Olanzapine; Seminal vesicle; mice
CPE - 1814
Determination of the presence of polysaccharide
capsule and resistance genes in pathogenic isolates
of Klebsiella pneumoniae
in the extracts were performed according to Matos (1988).
alkaloids. The proteolitic activity of gelatinases was visual-
hexane fraction increased the activity of gelatinase A in
droalcoholic fractions of extracts of specie of Vernonia
-
1. Universidade Federal de Pernambuco - UFPE, Brazil
2. Departamento de Parasitologia; Centro de Pesquisas Aggeu Magalhães - CPqAM / FIOCRUZ, Brazil
3. Laboratório de Imunopatologia Keizo Asami - LIKA / UFPE,
Brazil
4. Departamento de Medicina Tropical - Universidade Federal de
Pernambuco- UFPE, Brazil
cause urinary tract infections, septicemia, pneumonia and
meningitis, especially in hospitals environments. The most
action of gelatinases.
Keywords: Gelatinases, Zymography, plant extract
CPE - 1813
Effects of Olanzapine on the use of the seminal vesicles of mice Mus musculus
Silva, G. G. A.1; Amorim, M. J. A. A. L.2; Amorim Junior,
A. A. 3; Silva Junior, V. A.;4 Santos, C. R. O.5
1. Federal Rural University of Pernambuco - Brazil
2. Federal Rural University of Pernambuco - Brazil
3. Federal University of Pernambuco - Brazil
4. Federal Rural University of Pernambuco - Brazil
5. Federal Rural University of Pernambuco - Brazil
Background: Olanzapine is an atypical antipsychotic used
to treat acute and maintenance therapy in the frames of
schizophrenia and other psychoses, is currently marketed
90
of drugs for clinical treatment. Additionally, pathogenic
isolates of K. pneumoniae usually produce a large amount
of capsular polysaccharide (CPS), which confers resistance
resistant isolates of K. pneumoniae with ESBL phenotype.
Methodology: For this study, it was used four nosocomial
isolates of K. pneumoniae, the K15C, K15R, K16R e
-
2011 5 (2)
-
have the gene for production of CPS, showing high viruisolates at hospital environment. Conclusions: The isolates tested are highly virulent due to the presence of CPS.
main agent of the disease is Fonsecaea pedrosoi, followed
mated onset of the disease in approximately 163 000, con-
the infection treatment.
ride capsule
CPE - 1815
Association between multiple infections and vaccines
for human papillomavirus in women with cervical lesions
ation in the Brazilian population. Methodology: We con-
A.1, Silva-Neto, Jc.1.
1 - Laboratory of Clinical Cytology of the Federal University of
Pernambuco - Brazil.
Introduction: It is known that HPV is associated with
the prevalence of viruses HPV-6, -11, -16 and -18. However, other important oncogenic viruses, as well as associations of viruses in the same host can effectively participate
in the development of high-grade lesions and invasive.
pare with existing vaccines.
-
-
Keywords: Chromoblastomycosis; subcutaneous mycosis
CPE – 1816a
Congenital malformations in the municipality Caruaru-PE
1. Faculdade ASCES, Brazil
2. Universidade Federal Rural de Pernambuco, Brazil
3. Laboratório de Genética e Genômica, USA
Background: The Congenital Malformations are morpho-
the use of PCR-RFLP were detected HPV-DNA and the
samples were genotyped for viral types 6, 11, 16, 18, 31, 33
-
I, Hae III, Rsa I, Pst I) in a sample collected with cervical
-
-
-
maternity Jesus Nazareno in the municipality Caruaru-PE.
Methodology: A sectional study was performed on new-
-
according to affected systems according to codes Q00.0
outside the range.
congenital malformations were compiled in spreadsheets,
for the year and the period studied. Results: In the analysis
-
ies to analyze the spectrum of current vaccines immunization aiming at the detection of cross-reactions.
Keywords: vaccine, genotyping, multiple infections.
Analysis of congenital malformations showed a predominance of systems of musculoskeletal apparatus, followed
CPE - 1816
Chromoblastomycosis: a reality brazilian population
Silva Xavier, G.M.¹; Silva Melo, W.E.¹; Ferreira Júnior,
D.L.¹
1. Faculdade ASCES, Brazil
-
congenital deformities of the foot) presented the highest
tions of the musculoskeletal apparatus and genital system.
The prevalence of malformations in the municipality of
Caruaru and surrounding regions was lower, When com-
and verrucous plaques that may ulcerate. The fungus pen-
of information.
Keywords: Congenital malformations; Newborns;
Caruaru
ease is the most important part of the infectious diseases
CPE – 1816b
Implementation of a laboratory protocol for diagno91
Experimental Pathology and Health Sciences
sis of mucopolysaccharidosis
Silva Melo, W.¹; Xavier Silva, G.¹; Ferreira, C.S.¹; Esteves
1. Faculdade ASCES, Brazil
2. Laboratório de Imunopatologia Keizo Asami – LIKA/UFPE,
Brazil
Background: Mucopolysaccharidosis (MPS) are rare gecosaminoglycans (GAG). When they are not degraded,
variety of pathological complications, starting from the
fetal period until the infant stage. The diagnosis of MPS
ation. There are no epidemiological data on this disease
in Brazil, which makes recognition of the disease among
measuring enzyme in the investigation of MPS allows the
documented in the literature, misdiagnosis is common due
treatment.
Keywords: Paracoccidioidomycosis; Tuberculosis;
Diagnosis.
CPE - 1833
Evaluation of the in vitro leishmanicidal activity of
the ethanolic extract of Artemisia vulgaris
Barreto,M.M.L.1;
Soares,P.H.S.2;
Catena,A.S.1;
Soares,L.L.1; Almeida,E.R.2 ; Medeiros.P.L.1; Silva,E.C.1
1.Laboratório de Cultura de Tecidos/Depto. de Histologia e Embriologia - CCB/UFPE, Brazil 2.Depto. de Antibiótico - CCB/
UFPE, Brazil
treatment of leishmaniasis arises due the toxicity and the
emergence of resistant parasites to drugs already market-
of MPS. Methodology: The study was conducted through
To investigate the antileishmanial activity of the ethanolic extract of Artemisia vulgaris. Methodology: Artemisia
thin layer chromatography and research urinary GAG) and
fests and thus provide guidance in developing appropriate treatment protocols. The implementation of a proto-
of dry chopped leaves in 1L of ethanol. After 72 hours,
conducted in thin layer chromatography. Promastigotes
(Leishmania amazonensis and Leishmania major) were
Keywords: Mucopolysaccharidoses; Differential Diagnosis
(initial concentration), and the test was done in triplicate.
The ethanol was used as negative control. The parasites
CPE – 1816c
Paracoccidioidomycosis and tuberculosis: differential
diagnosis
Silva Melo, W.¹; Silva Xavier, G.M.¹; Oliveira, R.F.¹; Alves,
P.A.¹; Santana, K.S.¹; Almeida Sanatana, W.¹
1. Faculdade ASCES, Brazil
losis (TB) may occur simultaneously or sequentially. The
clinical presentation of disease can coexist, due to the
overlapping of the epidemiological and clinical stories. Er-
48 and 72 h. Results: Essential oil (linalool, cineol, thu-
form of two diseases and forged a consensus in the dif-
of sesquiterpene (vulgarina), tannin, resin and inulin were
nensis. Conclusion: the ethanolic extract of leaves of Artemisia vulgaris has leishmanicidal activity, which suggests
Keywords: leishmanicidal activity, medicinal herbs,
Artemisia vulgaris
CPE – 1834
Evaluation of wound healing potential of royal jelly
Costa, E.M.¹ Guimarães, B.J.E.1; Teixeira, R.R. 2; Pru-
Results: According to the data and articles analysed, TB
and PMC can compromise a single individual, and this asthe presence of the fungus P. Brasiliensis (PMC) or of the
-
92
R.I.M.A. 1
1. Laboratório de Biologia Celular e Mutagênese, CCO/UFSJ,
Brazil
2. Instituto de Genética e Bioquímica, Universidade Federal de
Uberlândia, Brazil.
Background: Wound healing corresponds to the tissue rejectives: To evaluate the effects of topical and oral use of
royal jelly in cutaneous wound healing. Methodology: 20
previously anesthetized mice Swiss were used. A circular
2011 5 (2)
mice with the use of scissors and forceps, and the wounds
were treated and measured daily for 20 days. Groups of 4
mice were given the following treatments: control (C- non
effect on Mg and K serum levels during acute phase leptospirosis in hamsters.
Keywords: Leptospirosis, Leptospira, Animal disease
models
CPE – 1837
control (C2). The wound tissue was processed for histological examination after 24 hours, 4 days, 7 days, 14 days,
and 20 days. Later, the slides were stained with hematoxylin and eosin (HE) and taken to optical microscope analysis. Results: In the RJC and RJO groups, on the 20th day,
for the other groups. As for edema formation, the RJO
group showed less formation. However, on the 14th day,
all animals showed very similar edematous aspect. From
which still showed it was the RJO. Dermal appendages
were seen earlier on animal of the VC group, with all of
them showing appendages on the 7th day. With relation to
re-epithelialization, the process occurred faster in groups
day. Conclusion: The use of topical or oral royal jelly does
not show any relevant interference in the healing process.
Keywords: wound healing, royal jelly, wounds.
CPE - 1836
Experimental leptospirosis in hamsters induce hypomagnesemia and hyperkalemia in acute-phase disease
Santos, C. S. 1,2; Azevedo, E. C. 2; Soares, L. M. 2;
Seixas,M. 1; Bandeira, M. 2; Amorim, D. M. 2; Chagas Jr,
A.D. 1;Alexander McBride, A. J. 1; Cruz McBride, F. W. 2;
Reis, M. G. 1,2; Athanazio, D. A. 1,2
1. Gonçalo Moniz Research Center, Oswaldo Cruz Foundation,
Salvador, Brazil.
2. Federal University of Bahia, Salvador, Brazil.
Introduction: Patients with severe leptospirosis develop
hypomagnesemia during acute phase disease as well as sodium and potassium wasting defects. The non-oliguric renal failure rapidly evolves to an oliguric hyperkalemic form
that denotes poor outcome. The renal waste of Mg, Na,
HPV by electrochemical biosensors
Zanforlin,D.M.L1,2,
Campos-Ferreira,D.S1,2,
Nascimento, G.A1,2, Arruda, M.S 1,2. Souza,E.V.M1,2,
Oliveira,N.C.L 1,2.Castelletti,C.H.M1,2, Bruneska,D.1,2,3,
Lima-Filho,J.L1,2,3
1. Federal University of Pernambuco, 2. Laboratory of Immunopathology Keizo Asami,LIKA, 3 Department of biochemistry of
Federal University of Pernambuco
Studies show that infections with human papillomavirus
(HPV) of high-risk can lead to the development of tumors
of the HPV is extremely important for a successful treat-
series of alignments among the HPV genomes to locate
consensus sequences of this virus. Finally, it was made a
-
found 26 consensus sequences. Among these, 7 sequences
The study comparative with the PapilloCheck showed that
tect 18 types of high-risk HPV. We could conclude that
detect HPV will have a simpler methodology than availKeywords: Biosensor, DNA, HPV
study the dynamics of Mg, Na and K serum levels during
acute experimental leptospirosis. Methods: Hamsters were
vals, for kidney lesions and evaluation of serum levels of
NO, K, Mg and Na. The intervals were 4, 8, 16 and 28 days
after infection. Hamsters were separated in four groups
according to the treatment started on the tenth day: ampi-
from day 4 to day 16. In addition, all groups experienced a
levels of Na remained unaffected among different interof serum levels of Na in the MB group at day 16. Conclusions: Hamsters reproduce the hypomagnesemia during
CPE – 1837a
Development of DNA probes to detect the serotype
DENV-4 using electrochemical biosensors
Oliveira,N.C.L
1,2,Zanforlin,D.M.L1,2,
CamposFerreira,D.S1,2 , Nascimento,G.A1,2 , Arruda, M.S 1,2.
Souza,E.V.M1,2, Lima-Filho,J.L1,2,3
1. Federal University of Pernambuco, 2. Laboratory of Immunopathology Keizo Asami,LIKA, 3 Department of biochemistry of
Federal University of Pernambuco
the world with approximately 100 million people infectfamily Flaviviridae, with 4 distinct serotypes (DENV-1
to DENV-4). Currently in Brazil the most prevalent seThe reemergence of this serotype in Brazil along with the
simultaneous circulation of other genotypes of dengue
increases the appearance of severe manifestations of this
disease, like dengue hemorrhagic fever (DHF).The early
ease and to take preventive measures. Herein, we report
production. Conversely, hypokalemia was not reproduced
in this model in which all groups developed increasing lev93
Experimental Pathology and Health Sciences
tional Center of Biotechnology Information (NCBI). The
-
of analyses of genome. The criteria for selection of the se-
portuguese), and the patients operated for esophageal
mismatches and hairpins.¬¬ As results, a total of 80 DNA
sequences were analysed. Among these, 15 were chosen as
-
Encephalopathy .Methodology: data collection took place
through research of collecting critical information developments and medical prescriptions and nurses, and clini-
detection of dengue.
Key-words: Dengue , DNA, electrochemical biosensors
CPE – 1838
Prevalence of Human Papillomavirus 35 in highgrade intraepithelial lesions and invasive female genital tract
F.1, Silva-Neto, Jc.1.
1 – Laboratório de Citologia Clínica da Universidade Federal de
Pernambuco – Brasil.
Cesário (normal), primary mother , who had hypertension crisis; the child had delay in development of crying
specialized hospital and staying therer during 34 days in
agnosed with GERD associated á ECNP, evolving with
Bronchopneumonia and Chronic Dyspnea, Pneumonia reranitidine, famotidine and nizatidine), evolving as well.
Conclusion: Although the infant have evolved positively to
prospective and longitudinal casuistic designs, with the
operated with DRGE and ECNP.
of the etiology of cervical cancer and the types 16 and 18
ated; not progressive chronic encephalopathy
HPV 6, 11, 16 and 18. Some studies report cross-reactions
with type 45, and others types 31 and 33. HPV-35 is not
included in the vaccination plan and do not suffer from
immunological cross-reactions.
in women with high-grade cervical lesions and invasive.
Methodology: We analyzed 89 samples of cervical lesions
on the type of HSIL and 37 invasive lesions (squamous
cell and adenocarcinoma). Through the use of PCR-RFLP
Results: Of 126 cases analyzed, HPV-35 was present in
CPE – 1840a
Aroeira do Sertão (Myracrodruon urundeuva fr. All):
assessment of the effects of Toxicology and healing
chemotype of essential oil Alpha-pinene
Silva, D. M. ¹; Silva, D. C. A. ¹; Cavalcanti, T. R. L ¹;Lima, S.
T. H. L.¹; Medeiros, J. H.¹; Sousa, M. A.¹
1. Federal University of Pernambuco, Brazil
2. Federal University of Rio Grande do Norte, Brazil
Introduction: Aroeira(mastic) is a common name for sevwhich the Myracrodruon urundeuva fr. Allemão is popularly known in Brazil as aroeira-do-sertão. These species
have many therapeutic uses, with actions such as ovarian,
Conclusion: The present study demonstrates that a sigHPV can is associated with type 35 which is not part of
the spectrum vaccine immunization now. The analysis of
spectrum are needed for the implementation of vaccination campaigns.
Keywords: PCR-RFLP, HPV genotyping, cervical lesion
oils D-Limonene (LIM) and Alpha-pinene (PIN) extracted
from the leaves of Myracrodruon urundeuva. Methodolsurgery. After the demarcation of the area, the skin was intotal resection. The animals were treated with PIN in the
(CRP), used in dilution of essential oils. Prior to the sacri-
CPE - 1840
ciated With Not Progressive Chronic Encephalopathy
Silva, D. M. ¹; Silva, D. C. A. ¹; Lira, M. C. C. ¹; Silva, L. G.
and total protein. Results: among the groups control with
Cremophor and distilled water there was statistical differ-
J. H.¹; Sousa, M. A.¹
1. Federal Universtiy of Pernambuco, Brazil
sociated with symptoms of diseases and pulmonary lower
airway such as aspiration pneumonia, asthma, chronic
94
sion: the use of essential oil for PIN in situ treatment of
2011 5 (2)
wounds, lifting discrete parameters reveals toxicity tested,
the treatment.
Keywords: aroeira-do-sertão; toxicity; healing.
CPE – 1841a
Promastigotes (Leishmania amazonensis and Leishmania
Cases of Pulmonary Tuberculosis in Children: An Integrative Review
Silva, D.C.A.1; Silva, D.M.1; Sousa, M.A.1; Silva, L.G.A.1;
S.Q.1
1. Universidade Federal de Pernambuco / Victoria Academic Center- UFPE / CAV, Brazil
the plates at concentrations of 62, 125, 250, 500 and 1000
triplicate, and DMSO was used as negative control. After
of life, especially in patients with HIV. It constitutes, nevcomplicates the the clinical suspicious and delayed diag-
manicidal activity against the species L. major and L. ama-
pulmonary TB in children are asymptomatic, while others
have cough for more than two weeks, fever, malaise and
Keywords: Leishmania major, Leishmania amazonensis, copaiba oil
integrative literature review as a method to this investigaish. Results: We selected 11 of 32 items found searching
Thus, according to scoring system (MSB) of health Min-
and limited effectiveness of diagnostic tests of childhood
TB, several scoring systems (score) were developed. However, these instruments have a wide variation in sensitivity,
oped a scoring system for the diagnosis of pulmonary tu-
CPE – 1843a
Segmentation of infections origin and topography in
patients admitted to the Emergency Clinic of a large
public hospital - Recife - PE
1. Microbiological Analysis Laboratory, Department. Pharmaceutical Sciences - Federal University of Pernambuco, Brazil
2. Hospital of the Restauração, Agamenon Magalhães Av S /
N, Brazil
Background: The emergence of new resistance and spread
indications aggravating infectious from hospitals or comstudy evaluated the incidence of infections, segmenting
reporting of cases.
Keywords: Pulmonary tuberculosis; childhood; diagnosis.
CPE – 1842
Leishmanicidal activity of commercial oil Copaiba
Barreto,M.M.L.1 ; Anjos, J.A.L2 ; Cassilhas, A.P.P3 ;
Amorim, R.V.S1 ; Silva, L.L.S1; Medeiros, P.L1 ; Silva, E.C1
1. Laboratório de Cultura de Tecidos/Depto. de Histologia e Embriologia - CCB/UFPE, Brazil
2. Núcleo de formação docente (NFD)/Centro Acadêmico do
Agreste (CAA)/UFPE/Brasil
3. University Salgado de Oliveira – UNIVERSO, Brazil
Background: Cutaneous leishmaniasis is an anthropoLeishmania. Parasitic infections are part of our regional
reality and therefore deserve special attention in the revarious species of the genus Copaifera (Leguminosae) and
Emergency Clinical Hospital of Restauração, which used
tion of infection was made according to the topography,
they were: respiratory, gastrointestinal, urinary, central nerthe community and the hospital. Results: With regard to
the work was done and need for future epidemiological
cording to the prevalence in origin, there was a predominance of infections of Community origin. Conclusions:
The results of the study justify the importance of continthe CCIH.
Keywords: Nosocomial Infections, Antimicrobial;
95
Experimental Pathology and Health Sciences
Hospital of Restauração
-
CPE – 1843b
Determination of tannins and total polyphenols and
toxicological study of the leaves of Schinopsis brasiliensis Engl. in the Artemia salina Leach
Batista, D.C.A.1; Castro, R.H.A.1; Coutinho, F.N.1; Pessôa,
ho, T.J.S.3; Amorim, E.L.C.3; Xavier, H.S.2; Pisciottano,
M.N.C.1*
1. Microbiological Analysis Laboratory, Department. Pharmaceutical Sciences, UFPE, Brazil
2. Laboratory of Pharmacognosy, Department. Pharmaceutical Sciences, UFPE, Brazil
3. Natural Products Laboratory, Department. Pharmaceutical Sciences, UFPE, Brazil
-
doses utilized in the acute toxicity. Calculation of the
using computerized linear regression analysis, in GraphPad
Prism, version 4.0. Results: All doses studied, analgesia was
the more predominant effect that was more pronounced
in animals treated intraperitoneally. The nerolidol presented toxic effects when administered i.p. The LD¬50
CNS with high toxicity when administered intraperitoneally and without apparent toxicity when administered orally
Keywords: Nerolidol, toxicity, psychopharmacology
screening
CPE – 1851a
-
rivative Im-7
polyphenols and tannins and evaluate the toxicity against
Artemia salina Leach methanol extract from leaves of S.
levels of total phenols and residual methanol extract from
za S. A. 2; Athayde-Filho, P. F. 2; Almeida, R. N. 1; Morais,
L. C. S. L. 1
1 .Laboratory of Pharmaceutical Technology, Health Sciences Center, Federal University of Paraíba
2. Chemistry Department, Federal University of Paraíba
ment of partial and generalized tonic-clonic seizures.
cating that the extract showed moderate levels of toxicity,
resulting in decreased movement of the larvae of Artemia
of tannins.
Keywords: Schinopsis brasiliensis; Artemia salina;
tannins
CPE - 1851
Pharmacological behavioral screening and acute toxicity of Nerolidol in mice
A. R. S.1; Salvadori, M. G. S. S.1; De Sousa, D. P.2; Morais,
L.C.S.L.1; Almeida, R.N.1
1- Laboratory of Pharmaceutical Technology, Federal University of
Paraíba , Brazil
2- Department of Physiology, Federal University of Sergipe, Brazil
Background: Nerolidol is a sesquiterpene present in the
toilet soaps and other toiletries as well as in non-cosmetic
jectives: The aim of this study was to carry out a phar-
anticonvulsant effect of the hydantoin derivative IM-7
(5-(4-isopropilfenil)-3-phenyl-imidazolidine-2,4-dione)
in pentylenetetrazole (PTZ) and maximum electroshock
(MES) induced seizures in mice. Methodology: Swiss al-
dard drugs for MES and PTZ tests, respectively). Thirty
(phenytoin and diazepam) or sixty minutes (vehicle and
IM-7) after treatment, animals were tested. In the PTZ
seizure were recorded. For MES test, mice received an
ear clip electrodes: seizure duration and mortality were
the parameters registered. Results: Doses of 150 and 300
121.8 versus 93.3 ± 11.5 sec in control). In MES test, the
zure (6.6 ± 1.5 versus 16.3 ± 0.7 sec in control) however,
kg. Conclusions: According to these results, high doses of
hydantoin derivative IM-7 showed an anticonvulsant effect that was associated with increased mortality. Further
weighing 25-35 g, approximately 3 months of age were
source of mortality.
Keywords: IM-7; Anticonvulsant effect; Toxicity.
administration (i.p.) in doses of 75, 100, 150, 175 and 200
CPE – 1851b
96
2011 5 (2)
Im-1, Im-3 And Im-7
za S. A. 2; Athayde-Filho, P. F. 2; Almeida, R. N. 1; Morais,
L. C. S. L. 1
1. Laboratory of Pharmaceutical Technology, Health Sciences Center, Federal University of Paraíba
2. Chemistry Department, Federal University of Paraíba
the presence of the imidazolidine ring in their structure,
have shown several pharmacological activities such as anticonvulsant, miorrelaxant, anti-tumor and anti-arrhythcontaining strips of paper was done after 24 hours at room
(5-(4-methylphenyl)-3-phenyl-imidazolidine-2,4-dione),
IM-3
(5-(4-etilfenil)-3-phenyl-imidazolidine-2,4-dione)
and IM-7 (5-(4-isopropilfenil)-3-phenyl-imidazolidine-2,4-
the isolates showed growth during the 06 months inventory, while the 12-month period, the rate of growth fell to
Keywords: Conservation, adsorption, bacteria
vous system. Negative results permit to reject the tested
hicle (distilled water and Tween 80) or different doses (50,
changes were evaluated comparing treated groups with
control group at 30, 60, 120, 180 and 240 minutes after
administration. Results: All doses of IM-1 reduced amduration of analgesic effect was increased according with
the doses (60, 120 and 180 minutes, respectively), and this
CEP – 1862a
Toxicological Reproductive Study of usnic acid from
Cladonia substellata in Famale Wistar Rats
Silva CR1, Silva MM1, Rodrigues MCS1, Santos KRP1,
Santos NPS1, 2, Aires CAM3, Silva RL4
1. Laboratory of Biotechnology and Pharmaceuticals / Universidade Federal de Pernambuco - Centro Acadêmico de Vitória, Brazil
2. Laboratório de Imunopatologia Keizo-Asami (LIKA), Universidade Federal de Pernambuco, Brazil
3. Institute of Biological Sciences, University of Pernambuco, Brazil
4. Faculdade Maurício de Nassau, Brazil
-
-
-
tion, lower touch response and ataxia from 120 to 240 min-
were investigated after the treatment of pregnant female
rats with the usnic acid. Methods: Twelve animals were
drugs. Further experiments are necessary to characterize
this effect and try to draw a structure-activity relationship.
Keywords: Hydantoin; Behavior; Depressant effect
via oral gavage, on days 6 at 10 of the gestation. Animals
from the control group received distilled water through the
same via. Th
signs of toxicity. Maternal characteristics were assessed as
-
CEP – 1862
Aires CAM1, Silva JD.2, Silva RL3, Silva CR.4
1. Institute of Biological Sciences, University of Pernambuco, Brazil
2. Brazilian Society of Clinical Analyses - PE, Brazil
3. Faculdade Maurício de Nassau, Brazil
4. Laboratory of Biotechnology and Pharmaceuticals - Centro
Acadêmico de Vitória - Universidade Federal de Pernambuco, Brazil
-
day 20 of gestation and fetuses were removed. The fetal
-
er treated females showed a re
characterization, conservation and use of microorganisms
are virtually essential for the development of processes for
22 isolates from different species or genus were grown in
24 hours prior to growth and 24 hours at room tempera-
control group. Conclusions: In conclusion, the evidence
points to an oral reproductive toxicity of usnic acid. News
cal studies.
Keywords: Lichen, usnic acid, toxicological reproductive
CEP – 1866
97
Experimental Pathology and Health Sciences
Effects of plant extracts in the skin wound healing
process
Franco, C. S. 1; Thomé, R.G. 2; Prudente, H. M. 1; Santos,
1. Laboratório de Biologia Celular e Mutagênese, CCO/UFSJ,
Brazil
2. Laboratório de Processamento de Tecidos – LAPROTEC,
CCO/UFSJ, Brazil
Background: Currently, a great diversity of plant extracts
were performed using scanning and transmission electron
tion indicator) releases of treated-cells was measured during two hours. All the extracts tested were active against
resulted in synergistic effects in eight ratios tested, while
extracts under experimental conditions is rarely demonextracts in the healing process. Methodology: An incision was made in male mice (Swiss) 4-6 weeks of age and
-
niques, and were stained with hematoxylin and eosin. For
analysis of wound healing, were considered the presence
extract on the cell surface. The protein release pattern was
L. ferrea and P. moniliformis anti-S. aureus actions. These
quantify the edema, was used a score: zero (physiological),
one (tenuous), two (moderate) and three (severe). The
Keywords: S. aureus; Anadenanthera colubrina , Libidibia ferrea and Pityrocarpa moniliformis
zero (physiological), one (tenuous), two (moderate) three
(severe) to four (very severe). Results: Regarding neoepi-
CEP – 1869
lagen in cutaneous scars in mice
for G1 animals presented closed wound while G4 showed
no re-epithelialization. Considering the skin appendages in
showed cutaneous structures while in G4 this appendages
-
1. Laboratório de Biologia Celular e Mutagênese, CCO/UFSJ,
Brazil
Background: The ulcer healing is a multifactorial phenomlular and molecular events in complex ways. The use of
recent years with the search for active ingredients that play
effective role in this phenomenon. For this reason, Brazil,
results compared to other treatments in the skin wound
healing process.
Keywords: Wound healing skin, Plantago sp and Siparuna sp extracts, medicinal plants
CPE – 1868
Caatinga plants as weapons against Staphylococcus
aureus: insights into action mechanisms and evaluation of synergetic effects with erythromycin
Cunha, CRA 1, Paiva, M 3, Araújo, JM 1, Figueiredo, RCB
2, Silva, MV 1, Correia, MTS 1.
1 Universidade Federal de Pernambuco, Brazil
2 Centro de Pesquisas Aggeu Magalhães, Brazil.
3 Centro de Tecnologias Estratégicas do Nordeste, Brazil
terium, developing increasing patterns of resistance which
ered the most important human pathogen in the twentyaureus action of hydroalcoholic extracts from fruits of
induced in mice and treated with plant extracts. Methodolof Swiss line in which were caused cutaneous lesions. The
daily treatment were applied topically and consisted of
lyophilized extracts of two species of the Plantago (G1)
and Siparuna (G3) in aqueous solution, one group received
the treatment, and another group received only water. The
daily application of the treatments were performed on top
the dorsal region of each animal. After 9 days of therapy
the animals were anesthetized and killed. Tissues were collected for histological analysis, with the purpose of quantiof the slides were acquired and the amount of collagen
was analyzed with the use of a microscope (Axio Scope.
A1 – Zeiss) coupled to a camera (AxioCam MRc5 - Zeiss)
and a software (Axiovision 4.8.1). The amount of collagen
was measured in comparison with the total area, expressed
in percentage.
Conclusions: Plantago sp and Siparuna sp did not intertion. The synergetic effects of extracts and erythromycin
98
process.
Keywords: wound healing, collagen
CEP - 1871
2011 5 (2)
Evaluation of collagen production during cutaneous
wounds healing treated with royal jelly: experimental
study
Guimarães, B.J.E. 2; Teixeira, R.R. 2; Prudente, H.M. 1,
Carvalho, M.M. 1; Ferreira, M. 1; Oliveira, R. J.3; Espin1. Midwest Campus, Federal University of São João Del Rei, Brazil.
2. Instituto de Genética e Bioquímica, Federal University of Uberlândia, Brazil
3. Molecular Oncology Research - Barretos Cancer Hospital, Brazil.
Background: Wound healing is a dynamic process which
relates a series of cellular and molecular events which aim
with signalizing pathway in cell growth and studies currently suggested that Na,K-ATPase mediated cell signaling
squamous cell carcinoma of oral cavity through immunohistochemistry assay and their relationship with tumour
positive. The immunostained was depending of histo-
work is to evaluate the production of collagen in cutaneous wounds in mice treated with topical and oral royal
jelly (RJ). Methodology: Mice were anesthetized to allow
removal of cutaneous tissue. Groups with 4 animals were
-
differentiated and poorly differentiated, respectively. Conclusion: Well differentiated tumour have high expression
-
no treatment to wound (C); and oral suspension treat-
with prognosis in oral cavity cancer.
RJ (C2). Treatment was carried out daily. The wound tissue
was processed for histological examination after 24 hours,
4 days, 7 days, 14 days, and 20 days. The slides were stained
es of the slides were acquired and the amount of collagen
was analyzed with the use of a microscope coupled to a
camera and a computer with the software Axiovision 4.8.1.
taken on each slide and the amount of collagen was ex-
CPE - 1877
Antigelatinolitic Activity of Plant Extracts Used in
Traditional Medicine
1. Laboratório de Biologia Celular e Mutagênese, CCO/UFSJ,
Brazil.
2. Molecular Oncology Research – Barretos Cancer Hospital , Brazil
Background: For the metastasis of cancer cells to occur,
MMPs, especially gelatinases (MMP-2 and 9), are impor-
compared to the control group (C2). The amount of collagen produced within this period corresponds to the colapproximately. Conclusions: In general, the treatment with
ertheless, results suggest that oral treatment with RJ poten-
crude extracts of plants on the activity of gelatinases and
-
inducing of wound.
Keywords: wound healing, collagen, royal jelly
-
CPE - 1876
subunit in squamous cell carcinoma of oral cavity
cancer
Silva, A. G.1; Costa, M.Z.O.4, Chaves, A.L.F. 4; Muniz, L.
1. Laboratório de Biologia Celular e Mutagênese, CCO/UFSJ,
Brazil
2. Laboratório de Processamento de Tecidos – LAPROTEC,
CCO/UFSJ, Brazil
3. Laboratório de Bioquímica Celular, CCO/UFSJ, Brazil
4. Grupo de Estudos e Pesquisas em Cancer de Cabeça e Pescoço UFSJ. Brazil
Background: Squamous cell carcinoma is the most comdifferentiated, moderately differentiated or poorly differentiated. However, studies regarding prognosis for this
-
Scion Image. Phytochemical tests to detect the presence
teroids, triterpens, coumarins, saponins and alkaloids were
performed according to Matos (1988). Results: The MMPtract. The extract of the species of the genus showed that
found in greater quantity in Astronium and Tapirira. The
research group is currently concentrating on carrying out
99
Experimental Pathology and Health Sciences
Keywords: Cerrado, Plants extracts, gelatinases
CPE - 1878
-
used to extract DNA and amplify the L1 conserved region
-
rlich tumor and popliteal lymph nodes in mice
Ferreira, A.S.1; Souto, C.M. 1; Pinto, F.C.H. 1; Alves, S.L.G.
HPV16 sequence was used as positive control and ul1. Laboratório de Biologia Celular e Mutagênese – UFSJ, Brazil
2. Laboratório de Patologia Comparada - LPC, - ICB/UFMG,
Brazil
3. Laboratório de Síntese Orgânica – CCO/UFSJ, Brazil
Background: Several synthetic molecules are used in anitures: a steroidal nucleus, an unsaturated lactone (jointly
growth of Ehrlich Tumor and popliteal lymph nodes in
mice after administration of synthetic cardiac glycosides
(DGB6). Methodology: Swiss mice were divided into three
imals received daily treatments. The animals in groups G1
respectively and the control group (C) received 0.01 ml of
saline throughout the experiment. Tumor growth was asof a caliper every two days until the end of the experipopliteal lymph nodes were removed for histopathological
the weights paw tumor, with the contralateral paw. Results:
groups. In the microscopic evaluation of tumors, G2
showed greater area of necrosis than other groups. There
tween the treated and untreated groups, visualized delicate
stroma and large cellular and nuclear pleomorphism. The
histopathological analysis indicated the presence of metastases in the popliteal lymph nodes in all groups. ConcluDGB6 did not alter tumor growth, the morphology of the
tumor mass and metastatic potential.
Keywords: Ehrlich tumor, cardiac glycosides
CPE – 1879
Retrospective evaluation of the incidence of HPV inby nested PCR
Silva, A.M.S.1; Limeira, C.2; Lima Filho, J.L.1,3; Magalhães, V.4; Bruneska, D.1,3; Araújo, R.F.F.1,3
1. Laboratório de Imunopatologia Keizo Asami-LIKA, Universidade Federal de Pernambuco, Brasil; 2. Hospital Oswaldo Cruz –
Universidade de Pernambuco, Brasil; 3. Departamento de Bioquímica – Universidade Federal de Pernambuco, Brasil; 4. Departamento
de Medicina Tropical – Universidade Federal de Pernambuco, Brasil
Introduction: Sexually transmitted diseases (STDs) pose to
that arise each year more than 340 million new cases of
Papilloma Virus (HPV), among men and women aged 15
to 49 years or in their more sexually active age. HPV is
-
of positive samples worked, demonstrating the presence
of the virus, although in lower percentage than expected.
Conclusion: The detection of HPV was detected using
tion of genomic material examined during the process of
Keywords: HPV; Cervical Cancer; nested PCR
CPE – 1881
Proliferating cell nuclear antigen (PCNA) expression
in induced lesions in mice skin treated with plant extract
1.Laboratório de Biologia Celular e mutagênese. CCO/UFSJ, Brazil.
2. Laboratório de Patologia Comparada - LPC, - ICB/UFMG,
Brazil
3.Laboratório de Processamento de Tecidos, CCO/UFSJ, Brazil
tion and remodeling. Several treatments and products have
To evaluate cell proliferation in skin, in the process of
healing, in mice treated with plant extracts. Methodology:
Swiss line in which were caused cutaneous lesions. The
daily treatment were applied topically and consisted of
lyophlized extracts of two species of the Plantago (G1)
and Siparuna (G3) in aqueous solution, one group received
the treatment, and another group received only water. The
daily application of the treatments were performed on top
the dorsal region of each animal. After 9 days of therapy
and sectioned (4µm) in microtome for mounting slides.
Immunohistochemistry was performed using a proliferation cell nuclear antigen (PCNA) at a concentration of
for the 4 groups: 27,18 + 2; 20,9 + 1,1; 15,4 + 1 e 12,9 +
2,3, Plantago, controle, Siparuna e pomade, respectively.
Conclusion: The application of the Plantago extract does
accelerate the healing process of open wounds in mice and
Keywords: PCNA, healing
of HPV in women with cervical cancer in the state of
100
CPE - 1884
Morphological aspects and osteogenic differentiation
2011 5 (2)
of mesenchymal stem cells cultured on new biomaterials
ros, L.A.1,2; Gondim, T.G.F.3; Bassoli, A.C.D.G.1; Silva,
L.L.S.1; Silva, E.C.1; Aguiar, J.L.A.4; Medeiros, P.L.1,2,3
1. Department of Histology and Embryology, CCB - UFPE, Brazil.
2. Postgraduate Program in Therapeutic Innovation, CCB - UFPE,
Brazil.
3. Postgraduate Program in Pathology, CCS - UFPE, Brazil.
4. Postgraduate Program in Surgery of Hospital das Clínicas CCS
- UFPE, Brazil.
identify the Methicillin Resistant Staphylococcus Aureus
(MRSA) in patients with soft-tissue infection treated at the
medical primary care of the Sanitary District II in Recife
-
-
-
consisting in the interview of patients who visited the
sion and drainage of the infection site. Results: There were
analyzed 119 patients. The most common lesion found
The medication class which were found more resistance
and osteogenic differentiation process of mesenchymal
-
cal procedure associated with an oral cephalexin or quinolone when necessary, is enough to treat the skin and soft
Keywords: Staphylococcus aureus, Methicillin Resistant, MRSA
for 5min, and then were centrifuged and resuspended in
CPE – 1885a
Determination and pharmacokinetics research of
mer sugarcane gel was used to evaluate the cell growth, adhesion and spreading. Results: The new supports sustained
the growth of cell colonies with elongated morphology
alho Neto, J.L.3,4; Aquino, T.M. 1; Alves, A.J.1.
1. Departamento de Ciências Farmacêuticas. Universidade Federal
de Pernambuco, Brazil,
2. Faculdade Pernambucana de Saúde, Brazil,
3 Hospital Mermorial Guararapes, Brazil.
4 Maxiclínica Médica, Brazil
rounded and irregular shapes. During osteogenic differendral aspects, so suggests a secretory function; already on
two weeks it was noted through SEM, a high density of
cells with extensive network of projections attached to the
mers of sugarcane used as new cell supports promoted the
adhesion and growth of MSCs, displaying one of the essential properties for future application in tissue engineerKeywords: biopolymers; mesenchymal stem cells; osteogenic differentiation
CPE – 1885
Methicillin Resistant Staphylococcus aureus in patients with skin infection
Alves, AQ 1; Alves, Rocha, R.M.F. 1, Alves, R.O.R1, Martins Filho, E.D.1; ALVES, AJ2
1. Faculdade Pernambucana de Saúde, Brazil,
2. Departamento de Ciências Farmacêuticas. Universidade Federal
de Pernambuco, Brazil
Background: Staphylococcus aureus (staph), is a common
exist and not all of them are harmful. MRSA is a strain
-
(HPLC) and to compare the pharmacokinetics parameters
healthy and nonsmoking volunteers were selected. The
study was conducted according with the current revision
of the Declaration of Helsinki. Each volunteer signed an
Ethical permission was taken to approve the protocol and
consent form of this study from the Ethical Review Committee of Hospital Memorial Guararapes. Blood samples
were collected from each volunteer over 36 hours after
2.0, 3.0 4.0, 6.0, 8.0, 12.0, 24.0, and 36.0 h. Results: Means
intervals are: 0, 1.032, 1.685, 2.026, 2.105, 1.949, 1.741,
-
is one of the most common causes of skin infections and
occasionally the cause of serious illness when affects the
Kurumin Linux software. Mean (Ttest) of area under the
101
Experimental Pathology and Health Sciences
-
physical exercise during development and analysis their
3.571 hr (93.58), and 0.204 and 0.207 hr-1 (91.61) for the
Linux and Pharmkit programs, respectively. Conclusions:
treadmill during 15-35 days of life. It consisted in 5 weekly
When the animals reached the age of 90-120 days they
parameters
CPE – 1885b
Validation of quantitative resorcinol method for meaA.J.1;
1. Departamento de Ciências Farmacêuticas. Universidade Federal
de Pernambuco, Brazil,
2. Faculdade Pernambucana de Saúde, Brazil,
3. Departamento de Antibióticos. Universidade Federal de Pernambuco, Brazil,
Results: Body weights at 7, 14, 21, 28, 35 days of life and
13.7±2.8g, 24.4±6.6g, 36.9±9.7g, 54.6±15.9g, 75.5±21.8g
and 283.6±41g, for the E group; and 14.9±3.7g, 25.7±7g,
41.8±8.3g, 62.4±13.8g, 77.3±19g and 314.5±32.2g, for the
ductive organs. The seminal vesicles produces a slightly
0.0025±0.0004g and 3.3500±0.2726cm, for E; and
0.7584±0.0825g, 0.0024±0.0002g, and 3.4857±0.3078cm
trophic and serial cartilage in the epiphysis were 1.56±0.24
and 3.36±0.59 for E; and 2.13±0.56 and 3.68±0.59 for S,
respectively. Conclusions: Physical exercise early in life can
-
tozoa). Fructose is also secreted from the accessory sex
Keywords: Exercise; Morphometry; Femur
found in sperm plasma. The fructose occupies the most
the semen or sperm affects more than one-third of the
To reduce the amount of hydrochloric acid and validation
of the methodology. Methods: Quantitative measurement
method. In this experiment the quantity of HCl was reagent (0,1g resorcinol and 0,1g of urea dissolved in 100ml
CEP - 1887
Behavior of hepatosplenic schistosomiasis mansoni
in the municipality of Zona da Mata Sul de Pernambuco
L.R.A1; Xavier, A.T1; Silva, G.R2; Domingues; A.L.C3
1 Universidade Federal de Pernambuco – Centro Acadêmico de
Vitória /CAV - Brasil
2 Programa de Pós-Graduação em Patologia – CCS/UFPE –
Brasil.
3 Universidade Federal de Pernambuco – Centro de Ciências da
Saúde/CCB - Brasil
Introduction: The situation of Schistosomiasis in Pernam-
-
disease prevalence in Brazil in recent years it still remains
546nm. Results: The standard curves were linear over the
form. This state has the highest mortality in the country.
Although new cases of disease present in expansion for
racy were investigated with quality control (QC) samples
Zona da Mata is still standing out for its high endemicity.
clinical aspects of carriers of the hepatosplenic schistoso-
the amount of HCl, this method is less expensive and less
Keywords: Infertility, fructose, semen, Selliwanoff
CPE - 1886
Body weight evolution and femoral bone features of
adult rats submitted to physical exercise during development
Vasconcelos, A.C.S.; Silva, T.C.O.; Melo, M.P.P.; Monteiro,
H.M.C.; Amancio-Dos-Santos, A.; Maia, L.M.S.S.
Universidade Federal de Pernambuco, Brazil
Background: Physical exercise includes all physical activity
that is programmed, structuralized and repetitive. It has
102
Cross-sectional study with 30 patients from this town at-
derwent previous treatment for portal hypertension with
2011 5 (2)
of cases representing the main co-existing infections in
this casuistry. Conclusions: The study suggests the onset
where to date there was no reports in the literature on the
developing of cancers, among them cancer of the cervix,
mortality rate. The polymorphism of glutathione S-transferase (GST) is closely linked to an increased risk of cervi-
need for continued vigilance of the Control Program for
the Schistosomiasis Eradication in the city.
Keywords: schistosomiasis mansoni, epidemiology,
California
study was to evaluate the risk of cervical injury in HPVpositive patients and correlate with GST polymorphism,
CPE – 1887b
Schistosomiasis mansoni: An educational and methodological approach in the state education in the
Zona da Mata de Pernambuco
Silva, P.C.V1; Serrano, S.Q1; Xavier, A.T1; Almeida, M.I1
; Vale, L.P.S1
1. Universidade Federal de Pernambuco – Centro Acadêmico de
Vitória/CAV - Brasil
were analyzed. PCR was performed using one of the genes
an intestinal parasite Shistosoma mansoni. It represents a
countries. In Brazil, the Northeast region has the highest
rates of endemic disease. The control of this disease is
was used as negative control. Electrophoresis in agarose
17 samples presented cervical lesions, 48 showed the presence of genes GSTM1 and GSTT1, 30 presented GSTM1
null genotype, 12 presented T1 null genotype and 10 had
water treatment, control of intermediate hosts, pharma-
(more than three children) alone increased 3 times the risk
of cervical lesions. The null GSTM1 genotype increased
cohol consumption there was an increase of this risk over
actions regarding the prevention and control of Schisto-
did not act as a risk factor in any of the correlations made.
Conclusions: The parity alone act as a risk factor for development of cervical injury, as well as null M1 genotype
de Santo Antão-PE. These students made up the Group 1
prior the educational activity (lecture) and Group 2 after
the action at different times. Teaching material (illustrated
studied included: education level, age, clinical form of
schistosomiasis, symptoms, treatment, prevention and in-
Keywords: papillomavirus; glutathione S-transferase;
cervical lesions
CEP – 1890
Evaluation Of Antitumor Activity Of Barbatic Acid
Of Cladonia Salzmannii In Sarcoma-180 Through
Histochemical Agnor Method
Santos, A.F.S.*; Filho, A.F.S.; Santos, A.B.S.; Farias, W.L.R.;
Falcão, E.P.S.; Júnior, F.C.A.A.; Santos,N.P.S
Universidade Federal de Pernambuco – Centro Acadêmico de
Vitória, PE, Brasil.
-
Educational activities were evaluated on four topics: cycle,
clinical presentation, treatment and disease prevention.
Group 2 had median of right answers higher than group 1
in 7 topics addressed. Conclusions: The educational pro-
tant indicator of tumor conduct is the cell proliferation,
-
associated to nucleonar organized regions (NORs), present in different amounts in malignant cells and normal
disease in the city.
Keywords: Health Education, Schistosomiasis mansoni, Pernambuco
technical AgNORs. Methodology: Tumor cells (5,0 x 106
CEP - 1889
Genetic polymorphism of glutathione s-transferase
as a risk factor for cervical injury alone and combined
with other factors in HPV-positive patients
Soares, A.J.O.1, Medeiros, A.L.P.1, Bruneska, D. 1,2, Lima
Filho, J.L. 1,2, Araújo,R.F.F.1,2
1. Laboratório de Imunopatologia Keizo Asami – LIKA/UFPE,
Recife-PE, Brasil
2. Departamento de Bioquímica –UFPE, Recife-PE, Brasil
region of mices Swiss (30-40g). 24 hours after inoculation
the other (control-group) received only NaCl solution
histological slides were visualized and photographed in
the control group (1,50 and 1,55 respectively), without
103
Experimental Pathology and Health Sciences
cells evaluated in animals treated as in the control group
tive capacity of the same.
Keywords: AGNORs, Barbatic acid, antitumor activity
CEP – 1890a
Effect Of N-Acetylcysteine On Schistosma Mansoni
perimental Schistosomiasis
Santos, A.F.S.1; Aires, A.L.1; Silva, A.G1. Filho, N.T.D.P.1;
Ramos, M.L.C.1; Araújo, S.B.D.1; Costa, V.M.A.1; Souza,
1. Universidade Federal de Pernambuco, UFPE
Introduction: Schistosomiasis mansoni is characterized
logical lesions. N-acetylcysteine (NAC) attenuates liver
clinical signs, especially when dogs showed atypical clinical
of the Immunohistochemical detection of Leishmania
(Leishmania) infantum in gastro intestinal tract of dogs.
Methodology: Fragments of duodenum, jejunum, ileum,
cecum, colon and rectum from 36 dogs with serological
tissue sections.Results: Amastigotes forms of L.(L.) infanmucosae. Conclusions: The Immunohistochemical detection of L.(L.) infantum in duodenum, jejunum, ileum, cecum, colon and rectum of naturally infected dogs showed
the parasitism of non-organ lymphatic tissue associated.
Keywords: Canine Medicine, Patholohy, Canine
leishmaniasis
CEP – 1915
detoxifying properties. Methodology: Swiss female mice
were percutaneously infected with 50 S. mansoni cerPZQ, NAC+PZQ and Control group that only received
ized after 59 consecutive days. Oral administration of 100
skin wounds in dogs: histopathological and morphometric study
Pereira Júnior, JR1; Menezes, FF1; Oliveira, VVG1; Silva
Junior, VA1; Coelho, MCOC1
1. Federal Rural University of Pernambuco, Brazil
-
ments the animals were perfused to recover worms and
fragments of intestine were used to evaluate the pattern
histological sections that were stained with Hematoxylin
and eosin and Masson trichrome. The samples were used
covered in controls and in animals treated with NAC, PZQ
and NAC+PZQ was 22.3 (±2.4), 15.0 (±1.9), 0.0 and 0.0
-
and morphometric aspects of skin wounds in dogs treated
ogy: Three lesions were produced in 18 dogs on the right
with calendula ointment, the median wound was treated
with vaseline and the caudal injury received saline soluthree times: seven, 14 and 28 days. For histopathological
and for morphometry was used an integrating ocular con-
of hepatic granulomas. In groups NAC and NAC + PZQ
spectively. numerical density of granulomas was reduced
in groups NAC, NAC + PZQ and PZQ, respectively. Conclusion: Mice infected with S. mansoni and treated with
NAC e NAC+PZQ showed reduction in parasite load, the
egg killed in intestinal tissue.
Keywords: Schistosomiasis, NAC, granuloma
CEP - 1901
Immunohistochemical detection of Leishmania
(Leishmania) infantum (Nicolle, 1908) parasites in
gastro intestinal tract of dogs
Ghinato, L.1; Maia, F.C.L. 2, Alves, L.C. 1; Tafuri, V.L. 3;
Figueiredo, M.M. 3; Torres, S.M. 1; Pimentel, D.S. 1
1. Laboratório de Doenças Parasitárias dos Animais Domésticos.
DMV/UFRPE, Brasil.
2. Laboratório de Patologia. DMV/UFRPE, Brasil.
3. Instituto de Ciências Biológicas. ICB/UFMG, Brasil.
Background: Canine leishmaniasis (CanL) due to Leishmaand some of the infected animals are asymptomatic. Di104
wounds treated until the 7th day there was no reepithelialization, however, the lesions which we used calendula
percentage of collagen. In the treatment of wounds up to
14 days there was no difference histopathologic and morphometric. By day 28 the experimental wounds showed
cellularity in the treatment with calendula ointment. Conpathologic
CPE – 1915a
Leishmania sp amastigotes in the female dog reproductive system
LC1; Silva Junior, VA1
1. Universidade Federal Rural de Pernambuco, Brazil
Background: Dogs are the most important reservoir of
2011 5 (2)
are the primary way of transmission. Although after the
infection the parasite multiply and migrate from lymphoid tissue to other organs included spleen, liver, and
tives: Immunohistochemical detection of Leishmania sp
amastigotes in the female dog reproductive system natuadult female dogs were referred to the outpatient clinic
Brazil) and diagnosed parasitologically positive for visceral
Fragments of the reproductive tract (vulva, vagina, cervix,
histopathology and immunohistochemistry diagnostic. All
were mounted on slides and stained with hematoxylin and
eosin (HE) or further processed for immunohistochem-
junctiva are presented in dogs with natural infection of
Leishmania infantum.
Keywords: Leishmania sp; Conjunctiva; Immunohistochemistry
CEP – 1918
Histopathological aspect and indication for surgery
in penile Squamous Cell Carcinoma in horse - Case
Report
Silva, L. T. R1; Maia, F. C. L1; Vaz, B. B. D1.
1. Universidade Federal Rural de Pernambuco- Dois Irmãos- Brasil.
Background: In the external genitalia cancer of horses,
squamous cell carcinoma is prevalent in second place
among skin tumors (VAN DEN TOP, 2008). The microgrowth of squamous cells invading normal tissue. The
keratin of the stratum corneum which is produced in the
form concentric laminated structures, known as keratin
-
structural change was a mild to moderate vulvar dermatipredominantly in the papillary layer of dermis. One of
dominantly composed of macrophages, plasma cells, and
many lymphocytes mainly in the connective tissue layer of
equine penile squamous cell carcinoma and its malignancy
and differentiation. Methodology: An equine Mangalarga
senting in the penis, a hyperplastic tissue covering every
aspect of the glans. There was also a permanent serous
moisture look at the site, as well as areas with myiasis. The
animal underwent partial amputation of the penis accord(1985), and then histopathological examination was per-
vaginal lesions related with Leishmania infantum infection.
Keywords: Leishmania sp; Female dog; Immunohistochemistry
mainly composed of polymorphonuclear cells and macrophages, non reactive. In other areas, there was intense ac-
CEP – 1915b
Immunohistochemical patterns of the conjunctiva of
dogs with natural infection of Leishmania infantum
Oliveira, VVG1; Guerra, NR1; Santos, EMS1; Severo,
MS2; Faustino, MAG1; Alves, LC1
1. Universidade Federal Rural de Pernambuco, Brazil
2. Universidade Federal de Sergipe, Brazil
Background: Canine Visceral Leishmaniasis (CanL) is a
There is proliferation of squamous epithelium, consisting
mainly of squamous cells, which often form pearls corneas, the cells form small or large clusters, usually nodular.
There is intense cellular and nuclear pleomorphism. Mitot-
megaly and lymphadenopathy.and ophthalmopathies.
histological and immunohistochemical patterns of the
conjunctiva of dogs with natural infection of Leishmania
infantum. Methodology: Five dogs positive to L.infantum.
according to serologic test (ELISA) presenting ocular alFragments of the conjunctives were collected and all tiswere mounted on slides and stained with hematoxylin and
eosin (HE) or further processed for immunohistochemistry. Results: The main structural changes was a mild to
ing from a focal or multifocal to diffuse process adjacent
to the epithelium. Lymphoid nodules presented enlarged,
however, amastigotes forms of Leishmania sp were not
visualized. Amastigotes forms of Leishmania sp, in turn,
The stroma is now thick, now it is thin, however, is always
well vascularized. Lymphocytes, macrophages and some
sions: Although the malignancy of this tumor, partial surgical removal of the penis provided the return to normal
activities a few months later.
Keywords: Carcinoma, penis, horse
CEP – 1918a
Feline and Canine Nasal Tumors: Retrospective Cytopathological Analysis in animals attended in veterinary hospital at Federal Rural University of Pernambuco, Brazil.
Silva, L.T.R1; Barros, R.B.1; Pereira, M.F1; Oliveira,
A.A.F.1
1. Veterinary Medicine Department, Federal Rural University of
Pernambuco (UFRPE), Brazil
common tumors reported in dogs are adenocarcinoma,
squamous cell carcinoma and chondrosarcoma. In the cat,
nasal tumors are less commonly diagnosed, correspondincluded. In this species, squamous cells carcinoma and
lymphoma are the most frequent tumors. Among those
105
Experimental Pathology and Health Sciences
ing the circulation of these isolates in the three units of
Analyze the frequency of nasal tumors in dogs and cats
pathology service of the department of veterinary medi-
gene. Conclusions: These results indicate a high polymorphism, high rate of resistance to methicillin and frequency
the animals. Methodology: Cytopathological diagnosis of
pathology area, in the veterinary medicine department of
perform this, records of the cytologic examination were
discarded. The data was transferred to spreadsheets consis, and were compared. Descriptive analysis was applied
Results: Of the 14 diagnoses of nasal tumor analyzed, 10
Keywords: Staphylococcus aureus; Exotoxins; Methicillin
CPE-1369
Prevalence of pressure ulcersand its related risk factors in patients in the unit of health of the municipality if Carpina-PE
Frota, M.T.N.C1; Oliveira, F.H.S2; Santos, E. R. R dos2;
Mello L.A.2, 3
2.Maurício de Nassau Faculty, Brazil.
3.Dentistry Faculty of Recife, Brazil.
frequently found tumors independently of the species
challenge for the health teams, demonstrating the importance of continuous updating of knowledge in this area
-
the prevalence of pressure ulcers in patients admitted to
Conclusions: The squamous cell carcinoma are the most
servational, descriptive, transversal and quantitative clinical
inspection through the skin, and monitor patients through
and cats.
Keywords: Pets; Intranasal tumors; cytology
CPE – 1926
Molecular characterization of Staphylococcus aureus
isolated from clinical specimens in Brazil
with a minimum stay of 24 hours of age or more than 18
none of the patients developed pressure ulcers within the
T.C. 3
1. Federal University of Pernambuco – UFPE, Brazil
2. Department of Genetics and Molecular Biology – PPGG/ Federal University of Pernambuco – UFPE, Brazil
3. Department of Microbiology- Oswaldo Cruz Foundation, Research Center Aggeu Magalhães - CpqAM/Fiocruz, Brazil
Background: Staphylococcus aureus is a major pathogen
associated with serious nosocomial diseases. Several types
staphylococcal exfoliative toxins (ETs) and toxic shock
syndrome toxin 1 (TSST-1). Around the world, including
methicillin-resistant S. aureus (MRSA) and therapeutic op-
site most frequently affected the sacral region. There was
a certain lack of unity in the essential materials for the
relation to the prevention and treatment of ulcers.
Keywords: Pressure ulcers, Risk Factors, Care of
Nursing
CPE-1872
Association of human papillomavirus genotypes with
p16INK4A protein expression and intraepithelial lesions in women in the state of Pernambuco - Brazil
a molecular characterization of S. aureus isolated from
Brazil. Methodology: A total of 40 clinical isolates were
analyzed from different sources of infection and hospital
departments. The Polymerase Chain Reaction (PCR) was
used for the detection of the genes of exotoxins and the
A.1, Silva-Neto, Jc.1.
1 - Clinical Cytology Laboratory of the Federal University of Pernambuco - Brazil.
Introduction: Approximately 40 genotypes of human papillomavirus (HPV) affect the genital tract, 15 of these are
associated with high oncogenic risk for developing cervical
The investigation of polymorphism of the coagulase gene
of the isolates. Results: All isolates showed the gene seg,
lease of cellular transcription factor E2F. The E2F acts in
the stimulation of cell replication and expression of the
tein p16INK4A.
traepithelial and invasive HPV genotyping and associate
p16INK4A protein expression in women from the interior
Material and methods: A retrospective cross-sectional
106
2011 5 (2)
and histology (85 lesions LSIL, 89 HSIL, 37 invasive squamous cell carcinoma), immunohistochemical detection of
p16INK4A protein and PCR-RFLP for genotyping of the
ture II HPV negative.
Results: Of the 211 cases studied the presence of
carbamate
Marques P1,3, Jorge M1,3, Cavalcanti K2, Farinha R3
1. Faculdade de Farmácia da Universidade de Coimbra
2. Universidade Federal de Pernambuco
3. Faculdade de Medicina da Universidade de Coimbra, Serviço de
Patologia Experimental
cide used in crops, trees and ornamental plants. The intake
Twenty Wistar mail rats, 8 weeks-old were randomly dis-
cell carcinoma were positive for p16INK4A prevalence of
complete necropsy was performed.
for high-risk HPV showed an association with high-grade
cervical lesions and invasive.
Keywords: HPV, p16, genotyping
CPE-1470
Morphometric Analysis of the Intestinal Mucosa in
Experimental Alcoholism
Marques P1,3, Jorge M1,3, Farinha R3, Cavalcanti K2
S3
1. Faculdade de Farmácia da Universidade de Coimbra
2. Universidade Federal de Pernambuco
3. Faculdade de Medicina da Universidade de Coimbra, Serviço de
Patologia Experimental
The impact of alcohol consumption is well known for
mechanisms involved in the alcohol consumption is sus2,5 million deaths per year1.
Twenty Wistar mail rats, 8 weeks-old were randomly disistration of wine incorporated in the diet in the dose of
thetics and the complete necropsy was performed.
evaluate the intestinal villi height (H).
The results of the measurements were divided in 5 groups:
evaluate the intestinal villi height (H).
The results of the measurements were divided in 5 groups:
the control group.
medium intestinal villi and high intestinal villi decreased
These results suggest that the administration of high doses
portant morphologic changes in the mucosa of small intestine, which may interfere with the physiology of this
organ.
CPE-1553
Evaluation of chronic treatment with 5-phosphodiesterase inhibitors on the prostate of mice
Gomes, F.O.S.1; Carvalho, M.C.1; Silva, B.S.1; Rocha,
Rodrigues, G.B1; Oliveira, A.G.V. 2; Peixoto, C.A1,2
1. Centro de Pesquisas Aggeu Magalhães- CpqAM/FIOCRUZ,
Brazil
2. Centro de Tecnologias Estratégicas do Nordeste- CETENE/
MCT, Brazil
phodiesterase-5 (PDE-5) used extensively for the treatment of erectile dysfunction. Recent studies have shown
-
group.
Comparing with the control group, in Group II was veri-
-
medium intestinal villi and high intestinal villi decreased
-
the treatment regimen, animals are euthanized in CO2
Results: Prostate sections from control group presented
-
CPE-1470a
Morphometric Analysis of the Intestinal Mucosa in
Experimental Intoxication With 1-naphthyl methyl107
Experimental Pathology and Health Sciences
papillary invaginations, as well as changes in the epithelium
and increase of secretion in lumen. ImmunohistochemiPKA, PKG and sGC immunopositivity. This same result
cation staining analyses were performed using the software
Graph Pad Prism v.05. Conclusions: According to our remolecular mechanisms of the mice prostate.
CPE-1562
LPSF/GQ-02 reduces the expression of matrix metalloproteinase-9 (MMP-9) and evolution of atheroSilva, A.K.S.1; Torres, D.O.C.1; Rocha, S.W.S.1; Silva, B.S.1;
Oliveira, F.G.1; Nunes, A.K.S.1; Lima, M. C. A.3; Galdino,
S. L. 3; Pitta, I. R.3; Peixoto, C.A1,2
1. Centro de Pesquisas Aggeu Magalhães- CpqAM/FIOCRUZ,
Brazil
2. Centro de Tecnologias Estratégicas do Nordeste- CETENE/
MCT, Brazil
3. Laboratório de planejamento e síntese de fármacos, UFPE, Brazil
Background: Experimental evidence suggests that matrix
metalloproteinase-9 (MMP-9) may play a causal role in
new-onset cardiovascular disease. Changes in the expres-
Keywords: matrix metalloproteinase-9, atherosclerosis, new drugs.
CPE-1568
in the central nervous system, in a multiple sclerosis
model
Nunes AKS1,2, Luna R2,Oliveira AGV1, Peixoto CA1,2,
Rapôso C1
1. Laboratório de Microscopia, Ministério de Ciência e Tecnologia,
Centro de Tecnologias
Estratégicas do Nordeste (MCT/CETENE)
2. Laboratório de Ultraestrutura, Centro de Pesquisas Aggeu Magalhães, Fundação Oswaldo Cruz, Recife, PE, Brazil.
tive effects in neurodegenerative diseases. Activation of
microglial cells may trigger the overproduction of sevjective: This study investigated the effects of chronic treata multiple sclerosis (demyelination) model. Methodology:
Controls received pure chow and water. After transcardiac
-
in the pathogenesis of several cardiovascular conditions
such as atherosclerosis, resulting in atherosclerotic plaque
-
16 on the expression of MMP-9 and evolution of atherodivided into four groups: 1-fed with high-fat diet (HFD)
-
ments were conducted for 10 weeks and in the last two
animals, comparing with control, indicating microglial
the aorta was quickly dissected and processed for optical
droplets inside, which characterize the foam cells. In the
the tunic media. Morphometric analysis demonstrated that
the treatment with pioglitazone did not reduce the area of
the atherosclerotic lesions when compared with the HFD
groups. Analysis of expression of MMP-9 showed that
the expression of MMP-9 compared with the HFD. Inexpression of MMP-9 compared with the HFD group.
promising candidate for the treatment of atherosclerosis.
108
pared to cuprizone group, indicating normal microglial
activation. Conclusions: The increased levels of cGMP,
modulator, regulating cytokines levels and protecting mineurodegenerative diseases.
CPE-1578
tion, necrosis and hemorrhage in carbon tetrachloride (CCl4)-induced liver injury in mice
Rocha, S.W.S.1; França, M.E.R1; Silva, J.C.2; Silva, B.S.1;
G.B.1; Oliveira, A.G.V. 2; Peixoto, C.A1,2
1. Centro de Pesquisas Aggeu Magalhães- CpqAM/FIOCRUZ,
Brazil
2. Centro de Tecnologias Estratégicas do Nordeste- CETENE/
MCT, Brazil
2011 5 (2)
Background: Pharmacological studies showed that Diethof DEC on the CCl4-induced hepatotoxicity in Swiss mice.
the animals were euthanized 24h later. Liver fragments
were processed for light microscopy and immunohistochemical assays. Results: Liver sections from control group
presented normal liver architecture, showed well preserved
tissue, composed of radially arranged cords of hepatoof the CCl4 group showed large areas of extensive necrose, mainly pericentral, loss of hepatic architecture, lipid
ministration completely prevented liver necrosis reduced
the DEC+CCL4 group showed minimal hepatic damage. Immunohistochemical analyses of the control group
other hand, strong TNF-expression was found in the CCl4
in pericentral areas. No apparent TNF-expression was
detected in nonparenchymal cells. DEC+CCl4 group furstaining analyses were performed using the image program
Gimp 2.6 software. Conclusions: According to present re-
of DEC on the molecular mechanisms involved in the inKeywords: Diethylcarbamazine, hepatocytes, anti-
109
Experimental Pathology and Health Sciences
110
2011 5 (2)
I LUSO-BRAZILIAN CONGRESS OF THE EXPERIMENTAL PATHOLOGY
XI International Symposium on Experimental Techniques
Federal University of Pernambuco
Selected Oral Presentacions
Oral Presentacions (O1)
Day 11/11/11 - 11.00 am -12.00 pm
Oral Presentacions (02)
Day 12/11/11 - 14 pm – 15.20 pm
CPE-1465a
Murine Experimental Model Of Chagasic
Megacolon
França Campos,C.; Cassin Duz, A.L.; Dantas Cangussu,
S; Veloso, V.M.2; Bahia, M.T.; Silva, T.A.; Arantes,
R.M. E.
CPE-1448
Evaluation of the mutagenic potential of essential oil from leaves of Rollinia leptopetala RE
Fries
Mangueira, V.M.; Brito, M.T.; Batista, T.M.; Moreira,
M.M.B.; Pessoa, D.R.; Viana, W.P.; Tavares, J.F.; Pessoa,
H.L.; Diniz, M.F.F.M.; Castello Branco, M.V.S.
CPE-1457
Gastroprotective Effect Of Cissampelos Sympodialis Eichl. (Menispermaceae): Role Of Sulphydryl Compounds And Nitric Oxide
Sales, I.R.P.; Lima, G.R.M.; Caldas-Filho, M.R.D.; Falcão, H.S.; Barbosa-Filho, J.M.; Batista, L.M.
CPE-1445
Essential oil from leaves of Lippia microphylla
Cham.: antitumor effect and histophatological
study
Batista, T.M.; Moura, A.P.G.; Lunguinho, D.M.; Brito,
M.T.; Xavier, A.L.; Pita, J.C.L.R.; Silva, T.G.; Tavares,
J.F.; Diniz, M.F.F.M.; Castello-Branco, M.V.S.
CPE-1399
Mutagenic potential evaluation of hydroalcoholic extract of Zornia brasiliensis (Fabaceae)
Moreira, M.M.B.; Batista, T.M.; Brito, M.T.; Mangueira,
V.M.; Rolim, T.L.; Tavares, J.F.; Silva, M.S.; Pessoa,
H.L.; Diniz, M.F.F.M.; Castello Branco, M.V.S.
CPE-1392
Salivary Glands in Experimental Intoxication
with DMBA
Nery da Silva, E., Alves da Silva, M.A., Araújo Félix
Junior, J., Pereira, F., Farinha Henrique Marques, R.H.,
Silvério Cabrita, A.M., Paiva Figueiredo, A.S. , Carvalho
Freitas Ramos, T.
CPE-1446a
In vitro and in vivo antitumor activity of es(Annonaceae)
Sousa, T.K.G.; Macêdo, B.V.F.L.; Lunguinho, D.M.; Brito, M.T.; Moura, A.P.G.; Pita, J.C.L.R.; Xavier, A.L.;
CPE-1439a
Chemical composition and antitumor activity of
essential oils of species from Northeast of Brazil
Pessoa, D.R.; Xavier, A.L.; Lunguinho, D.M.; Moura,
A.P.G.; Brito, M.T.; Costa, V.C.O.; Pita, J.C.L.R.; Tavares, J.F.; Diniz, M. F. F. M.; Castello-Branco, M.V.S.
CPE-1436
An alternative method to induce experimental
pulmonary infection by Paracoccidioides brasiliensis in mice
Ribeiro, RSA; Cardoso, RA; Teixeira, GAPB; Dias, EP;
Figueiredo Júnior, I; Verícimo, MA
CPE-1728
Histomorphometric Analysis Of Skin Wound
Healing Undergoing Acupuncture Therapy:
Study In Rats
Souza, M.F.A.M.; Silva, E.L.V.; Santos, J.A.A.; Freire,
F.J.A.;Pinto, R.H.; Aguiar Jr, F.C.A.
111