th
XI Reunião Regional Nordeste da SBBq
4 International Symposium in Biochemistry of Macromolecules and Biotechnology
Recife – PE, 5 a 7 de dezembro de 2012
STATISTICAL EVALUATION OF WOUND HEALING IN RATS USING THE
FODDER PALM MUCILAGE (Opuntia ficus indica)
Silva, C.S.1; Santos, M.M.2; Uchôa, A.D.A.2; Silva, E.P1; Ribeiro, M.H.M.L.2;
Cadena, P.G.1; Pimentel, M.C.B.2; Silva, M.P.C2.
1
Laboratory of Immunopathology Keizo Asami- LIKA, UFPE, PE, Brazil.
Department of Biochemistry, Laboratory of Immunopathology Keizo Asami- LIKA,
UFPE, PE, Brazil.
2
The literature describes that the extracts of Opuntia ficus indica cladodes are
widely employed in folk medicine for the treatment of skin and epithelium wounds
and of mucous membrane irritation. In order to give this a scientific basis, the
present study aimed to evaluate the efficacy of fodder palm mucilage in the wound
healing, by using statistical analysis. The palm was collected at the IPA
experimental station in Caruaru-PE and then prepared its mucilage. The
experiment was performed in triplicate. 24 rats, albino Wistar females were used,
totalizing three groups where each one consisting of eight animals, four control
and four tests. Trichotomy in the dorsal region was performed inducing a wound
diameter of 5 mm and then applied the experimental treatment 0.5 ml of the palm
mucilage and the control Ointment Dermasin ® (silver sulfadiazine) in each rat.
During the period of 14 consecutive days it was conducted topical treatment and
daily assessment of injury. On days 3, 6, 10 and 14 after surgery, euthanasia
occurred and tissue samples were collected for histological analysis. Statistical
evaluation was done by Tukey test and nonparametric Mann-Whitney, considered
p <0.05. The process of tissue repair in animals treated topically with the mucilage
present a better response compared to the control. These results were confirmed
by statistical comparison between the different days of treatment and histological
scores, that showed a statistically significant difference (p <0.05).
Word Keys: Opuntia ficus indica, Mucilage, Wound healing
Supported by: CNPq, LIKA, UFPE.