POTENCIAL DA ESPECTROSCOPIA DE FLUORESCÊNCIA INDUZIDA POR LASER PARA
SEPARAR VARIEDADES DE LARANJA GENETICAMENTE PARECIDAS
T.M.K. Kubota1, A.B. Magalhães2, M. Cristofani-Yaly3, P.R. Vilas-Boas2, D.M.B.P. Milori2
(1) Universidade de São Paulo, Avenida Trabalhador São Carlense, 400, 13560-970, São Carlos, SP,
[email protected]
(2) Embrapa Instrumentação, XV de Novembro, 1452, 13560-970, São Carlos, SP, [email protected],
[email protected], [email protected]
(3) Centro APTA Citros Sylvio Moreira, IAC, Rodovia Anhanguera Km 158, 13490-970, Cordeirópolis, SP,
[email protected]
Resumo: As laranjas doces (Citrus sinensis L. Osbeck) concentram as espécies mais importante do grupo
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pigmentadas, comum, de baixa acidez e de umbigo. Entre esses grupos existem grandes diferenças fenotípicas,
como a forma do paio, cor, tamanho do fruto e época de maturação do fruto. Apesar de todas estas diferenças
fenotípicas, existe uma grande semelhança entre os genótipos destas espécies. Nota-se uma pequena variabilidade
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diferenciar estas variedades, porém com pouco sucesso. Pensando nisso a Embrapa Instrumentação junto com o
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induzida por laser e algumas ferramentas estatisticas. Os resultados são obtidos de forma rápida, sem preparo
de amostras e com baixo custo. Neste trabalho foi utilizado um conjunto de folhas de tres variedades de laranjas
genéticamente parecidas: Laranja Pera Abril, Laranja Pera Bianchi e Laranja Pera Roberto Gullo. Estas folhas
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partir de uma validação cruzada, foi possível separar corretamente as três classes de Laranja Pera com um acerto
de 88 %.
Palavras-chaveHVSHFWURVFRSLDÀXRUHVFrQFLD3KRWRQ&LWUXVODUDQMDVGRFH
POTENTIAL OF THE LASER INDUCED FLUORESCENCE SPECTROSCOPY IN SEPARATING CITRUS
VARIETIES VERY CLOSE GENETICALLY
Abstract: The sweet oranges (Citrus sinensis l. Osbeck) concentrate the most important species of the citrus
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common, low acidity and navel oranges. Major differences between these groups are the phenotypic size, shape
of the paio, its color, size of the fruit and the fruit maturation season. Despite all these phenotypic differences,
there is a large similarity between the genotypes of these species. It is possible to observe a small molecular
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these varieties, but they were not successful. Thinking about this, the Embrapa Agrocultural, together with the
Instituto Agronômico de Campinas (IAC), has developed a new technique to perform the differentiation between
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can be applied to a large number os samplesandprovide a quality database. In this work, it was used a set of leaves
of three different orange varieties genetically simlar: “Laranja Pera Abril”, “Laranja Pera Bianchi” and “Laranja
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&LWUXV:LWKWKHDLGRIWKH:HNDVRIWZDUHVWDWLVWLFDOWUHDWPHQWVZHUHPDGHIURPWKHÀXRUHVFHQFHVSHFWUDRIWKH
leavesand it was possible to separate the three classes correctly, with a success rateof 88%.
KeywordsVSHFWURVFRS\ÀXRUHVFHQFH3KRWRQ&LWUXVVZHHWRUDQJHV
1. Introdução
No setor agropecuário a produção de citros tem grande destaque econômico. São produzidos aproximadamente 122 milhões de toneladas de frutas, que correspondem a 17 bilhões de dólares em vendas da fruta fresca
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T.M.K. Kubota, A.B. Magalhães, M. Cristofani-Yaly, P.R. Vilas-Boas, D.M.B.P. Milori
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UHVSRQViYHOSRUJHUDUPDLVGHPLOHPSUHJRVGLYLGLGRVHPPDLVGHPXQLFtSLRV1(9(6
$VODUDQMDVGRFHVCitrus sinensis /2VEHFNFRQFHQWUDPDVHVSpFLHVPDLVLPSRUWDQWHGRJUXSRGRVFLWURVFXMDSURGXomRPXQGLDOHPFKHJRXDPLOWRQHODGDV)$2(ODVVmRFODVVL¿FDGDVFRPRODUDQMDV
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+(55(52
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2. Materiais e Métodos
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51
3. Resultados e Discussão
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4. Conclusões
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T.M.K. Kubota, A.B. Magalhães, M. Cristofani-Yaly, P.R. Vilas-Boas, D.M.B.P. Milori
Agradecimentos
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Referências
)22'$1'$*5,&8/785(25*$1,=$7,212)7+(81,7('1$7,216)$2 Produção total de citros
em 2012'LVSRQtYHOHPKWWSZZZIDRRUJ!$FHVVRHP VHW
1(9(60)HWDO2UHWUDWRGDFLWULFXOWXUDEUDVLOHLUD'LVSRQtYHOHP
KWWSZZZFLWUXVEUFRPEUGRZQORDGELEOLRWHFD$SUHVHQWDFDR0DUFRV)DYDHYHQWRYDORUSGI!
$FHVVRHPVHW
+(55(52 5$6Ë16 0- &$5%21(// ($ 1$9$552 / *HQHWLF GLYHUVLW\ LQ WKH RUDQJH VXEIDPLO\$XUDQWLRLGHDH ,QWUDVSHFLHV HQG LQWUDJHQXV JHQHWLF YDULDELOLW\ 7KHRUHWLFDO DQG$SSOLHG *HQHWLFV Y S 0,/25,'pERUD0DUFRQGHV%DVWRV3HUHLUD5$<1$8'0LFKHO%2$63DXOLQR5LEHLUR9LOODV9(1Æ1&,2
$QGUp/HRQDUGR0281,(56WpSKDQH%$66$1(=,5HQDWR%HR]]RH5('215RODQG,GHQWL¿FDWLRQRIFLWUXVYDULHWLHVXVLQJODVHULQGXFHG)OXRUHVFHQFH6SHFWURVFRS\/,)6&RPSXWHUDQG(OHFWURQLFVLQ$JULFXOWXUH
YS
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