rd
23 Congress of the International Union for Biochemistry and Molecular Biology
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44 Annual Meeting of the Brazilian Society for Biochemistry and Molecular Biology
th
th
Foz do Iguaçu, PR, Brazil, August 24 to 28 , 2015
METALLIC FLOW DIFFERENCES BETWEEN OXIDATIVE AND
NEURODEGENERATIVE STRESSES IN SUBCELLULAR FRACTIONS OF MOTOR
NEURON
Lago,L.1; Batista,B. L.1; de Oliveira Souza,V.C.2; Cipriano,S.S.1; Barbosa,F.2;
Cerchiaro,G.1
1
Centro de Ciências Naturais e Humanas, Universidade Federal do ABC, Santo André,
Brazil; 2 Departamento de análises clínicas, Toxicológicas e Bromatológicas, Faculdade
de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Ribeirão
Preto, Brazil;
Some metals have been associated to several diseases related to oxidative and
neurodegenerative stresses but there is no clue about metallic flow of these two types
of stresses. Additionally, the metallic influx and imbalance in subcellular fractions
remains unknown during the oxidative or neurodegenerative conditions. The aim of this
study was to determine the metallic flow in neurodegenerative and oxidative treatments
in motor neuron cell culture.
The concentration of 16 metals (Cu, Fe, Zn, Cr, Co, Ca, Ba, Cd, Mn, Mg, Pb, Se,
Rb, Ni, Bi, Be, As) was quantified by inductively coupled plasma mass spectrometry in
isolated subcellular fractions (nuclei, mitochondria and whole neuron) of motor neuron
in culture. Two conditions were analyzed: oxidative stress (OS) induced by H2O2 (500
µM) and neurodegenerative stress (NS) induced by αβ-Amyloid (50 nM). The NS had a
rapid metallic migration at 24 hours in nuclei (Ba, Cr, Co, Pb), in mitochondria (As) and
in the whole neuron (Ca, Se), while the OS had the same behavior but at 48 hours of
treatment. The mitochondrial response for some metals (Mn, Fe, Se, Bi, Mg, Ca)
occurred preferentially at 24 hours for both treatments.
Measurements of metallic flow indicated some specific behaviors according to
the treatment. For NS it was observed the following changes: Fe, Cu, Ca and Rb in
nuclei; Ni, Rb, Zn and Ca in mitochondria and Fe, Co, Ba, Zn and Cd in whole neuron.
And as for OS was observed the following changes: Mn and Rb in Nuclei; Rb, Pb, Be
and Zn in mitochondria and Fe, Cu, Be, Cr, Pb, As, Ni, Ba, Co, Zn, Rb and Ca in whole
neuron.
This study is important to determine the metallic flow in different stresses
conditions in motor neuron. Next steps will be to determine how metalloproteins are
involved with these flows.
Keywords:.Metallic Flow; Neurodegenerative Stress, Oxidative Stress.
Supported by: FAPESP, CNPq.
Supported by: FAPESP, UFABC, CNPq.
Brazilian Society for Biochemistry and
Molecular Biology (SBBq)