1st Workshop on Multi-User-Equipment and Facilities
High Resolution Mass
Spectrometry Lab
IQSC-USP-São Carlos – Principal Investigator and
Presenter: Emanuel Carrilho
HRMS LTQ Orbitrap Velos
EMU – PROC No.
2009/54040-8
• http://lcms.iqsc.usp.br/about/
• http://lcms.iqsc.usp.br
• http://lcms.iqsc.usp.br/eventos/
• http://lcms.iqsc.usp.br/infraestrutura/
Original Proponents IQSC/USP
Coordenador: Emanuel Carrilho
Pesquisadores Associados: André Luiz Meleiro Porto
Antonio Carlos Bender Burtoloso Eny Maria Vieira
Daniel Rodrigues Cardoso
Júlio Cesar Borges
Pesquisadores Colaboradores: Agnieszka Joanna Pawlicka Maule
Benedito dos Santos Lima Neto
Douglas Wagner Franco
Janete Harumi Yariwake Marcos Roberto de Vasconcelos Lanza
Roberto Gomes de Souza Berlinck
Ubirajara Pereira Rodrigues Filho
Vitor Marcelo DeMlon
Steering Committee
• Emanuel Carrilho (IQSC)
• Edson Rodrigues Filho (UFSCar)
• Daniel Rodrigues Cardoso (IQSC)
USER’s Committee
• Antonio Carlos Bender Burtoloso (IQSC)
• João Batista Fernandes (UFSCar)
• Quézia Bezerra Cass (UFSCar)
• Janete Harumi Yariwake (IQSC)
• Maria de Fátima G. F. da Silva (UFSCar)
• Regina Vincenzi Oliveira (UFSCar)
High Resolution Mass
Spectrometer LTQ Orbitrap Velos
•
•
•
Ionization sources:
•
•
•
•
electrospray ionization (ESI)
nanoelectrospray (nanoESI)
atmospheric pressure chemical ionization (APCI)
desorption electrospray ionization (DESI)
Separation units:
•
ultra-performance liquid chromatography (UPLC) Accela High Speed LC
(Thermo Scientifc)
•
nano-LC EASY-nLc II (Thermo Scientific)
Sample Prep Accessories:
•
•
•
Akta Prime GE (preparative scale sample prep)
Liophilizer Alpha 2-4 LDplus Christ
Concentrator model RVC 2-33IR CD Plus Christ.
Location & Installations
Location & Installations
Location & Installations
Location & Installations
Location & Installations
Equipment
Supplies
Accessories @ UFSCar
Accessories @ UFSCar
Separations Units
Separations Units
Ionization Sources
Ionization Sources - DESI
Ionization Sources - NanoMate
CAPES - Pro-Equipamentos
RT#Expenditure#
training'
so*ware'
reagent'
infra'
consumables'
accessory'
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Scheduling & Usage
35!!
30!!
5$$ 5$$ 4$$2$$
25!!
20!!
15!!
iqsc$
23$$
ufscar$
61$$
fmrp$
165$$
embrapa$
exterior$
unifesp$
unesp$
10!!
5!!
!!
Future Charges After Final Report
Direct Infusion - per Sample
$ 10.00 (IQSC & UFSCar)
$ 25.00 (Academic)
$ 125.00 (Industry)
NanoLC and UHPLC MS
$ 75.00/day (IQSC & UFSCar)
$ 125.00/day (Academic)
$ 125.00/sample (Industry)
100% Granted EMU
• 2004: CE-MS (w/ Marina FM Tavares IQ/USP and
Adelia R Amaya, UNICAMP)
• 2004: LC-QToF (Fernando M Lanças and Douglas
W Franco, IQSC and Edson Rodrigues Fo, UFSCar)
• 2009: HRMS LTQ Orbitrap Velos (w/ Edson
Rodrigues Fo, UFSCar)
• 2014/2015: Tribrid Orbitrap Fusion; MALDI/ToF
Imaging; SEM-FEG; GCxGC/MS
Thanks