Differential Resistance Mutation and Polymorphism
Acquisition in HIV-1 Protease of Subtypes B and F1
from Children and Adult Patients
Ana Teresa Dumans, Cláudia C. Barreto, André F. Santos, Mônica B. Arruda, Thatiana M. Sousa,
Esmeralda A. Soares, Adriana O. Afonso, Elizabeth S. Machado, Ester C. Sabino, Alberto Duarte,
Rodrigo M. Brindeiro, Amilcar Tanuri and Marcelo A. Soares
METHODS
• Subtype B and F1 PR sequences were matched by average
exposure time to PI.
• Demographic and clinical data were take into account as
much as possible.
• Statistical analyses were performed with Fisher´s exact,
Student t and Mann-Whitney U tests, p < 0.05 was considered
significant.
RESULTS
• Subtype F1 contained a
higher proportion of other
polymorphisms.
• V82L was present in isolates
from children.
• In untreated patients, L63P,
A71T, and V77I were more
prevalent in subtype B, while
L10V, K20R, and M36I in
subtype F1.
• In treated patients, K20M,
D30N, G73S, I84V, and
L90M were more prevalent in
subtype B, while K20T and
N88S in subtype F1.
CONCLUSIONS
• Differences in treatment regimens and in
viral replication rates may play a role in
the differences between drug resistance
acquisition in adults and children.
• Rates of overall drug resistance did not
differ between subtypes B and F1 but
subtype B accumulates resistance at
higher proportion in specific amino acid
positions.