Presentation:
1. BIOEN research program
2. Sugarcane genome sequence
• M. A. Van Sluys
• Botânica – IB – USP
• [email protected]
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Energy sources in Brazil, 2006
Brazil: in 2009 47% energy from
renewable sources; sugarcane 18%
60%
50%
40%
30%
20%
cane
18%
10%
0%
Non-Renewable
Renewable
Brasil: 47%; Mundo: 13%; OECD: 7,2%
Foto Capa:
Leo Ramos
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BIOENERGY DRIVERS
Energy Security
Sugarcane bioethanol
contributes to 20% of
the Brazilian liquid
fuels matrix
Biomass cogeneration
can contribute with
up to 18% of Brazil’s
electricity demand
Sustainable
Development
Environmental
Security
The sugarcane
industry contributes
to agriculture
modernization, rural
development,
improved education
and the creation of
jobs
The use of Sugarcane
bioethanol can reduce
CO2 emissions by 80%
when compared to
gasoline
Opportunities for
innovation
Biofuel certification
can contribute to the
reinforcement of
agroecological zoning
Food Security
Sugarcane production
for energy did no
decrease food
production
Expansion is occuring
mainly in pasture land
Only 0.5% of brazilian
land used to produce
bioethanol
BIOEN DIVISIONS
BIOMASS
Contribute with knowledge and technologies for Sugarcane Improvement
Enable a Systems Biology approach for Biofuel Crops
BIOFUEL TECHNOLOGIES
Increasing productivity (amount of ethanol by sugarcane ton), energy
saving, water saving and minimizing environmental impacts
ENGINES
Flex-fuel engines with increased performance, durability and decreased
consumption, pollutant emissions
BIOREFINERIES
Complete substitution of fossil fuel derived compounds
Sugarchemistry for intermediate chemical production and
alcoholchemistry as a petrochemistry substitute
SUSTAINABILITY AND IMPACTS
Studies to consolidate sugarcane ethanol as the leading technology path
to ethanol and derivatives production
Horizontal themes: Social and Economic Impacts, Environmental studies
and Land Use
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FAPESP Bioenergy Research Program BIOEN
BIOEN Program : 5 Divisions
Fundamental knowledge & New technologies
for a bio-based society






Academic Basic and Applied Research (US$ 40 million)
Since 2008, 136 grants, 400 brazilian researchers, collaborators
from 15 countries
Regular, Theme and Young Investigator Awards
Open to foreign scientists who want to come to Brazil
State of São Paulo Bioenergy Research Center (US$ 90 million)
FAPESP, USP, UNICAMP, UNESP, State of São Paulo
Government (80 new faculty positions for bioenergy
researchers)
Creation of a Bioenergy PhD Program
Partnerships
 United States, United Kingdom and The Netherlands, Brazil
 Oak Ridge National Laboratories, UKRC, BBSRC, BE-Basic,
GSB, LACAF, BOEING, BP, Braskem, Dedini, ETH, Microsoft,
Oxiteno, PSA Peugeot Citroën, Vale
Innovation Technology, Joint Industry-University research (5 years)
Innovation Centers, Joint Industry-University centers (10 years)
Company
Subject
Oxiteno
Lignocellulosic materials
Braskem
Alcohol-chemistry
Dedini
Processes
ETH
Agricultural practices
Microsoft
Computational development
Vale
Ethanol technologies
Boeing
Aviation Biofuels
BP
Processes and sustainability
PSA
Engines
Australia
Austria
Belgium
China
Denmark
Finland
France
Germany
Guatemala
Italy
Portugal
Spain
The Netherlands
United Kingdom
United States
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15 PITEs and 12 PIPEs
Biomass Research
Precision agriculture
Tractors
Prediction of sugarcane yield
Pest management
Assembly of the sugarcane polyploid genome
Engines
Flex-fuel engines
Ethanol spray in internal combustion engines
Biofuel Technologies
New Saccharomyces cerevisiae strains from the Brazilian
biodiversity
Grinding process
Bagasse delignification
Bagasse hydrolysis
High performance fermentation
Sustainability
Atmospheric CO2 mitigation
Agricultural GHG emissions
Waste machines
Waste germination and plantation tubes
Biorefineries
Nanofibers from renewable sources
Biodegradable polymers
Acrylic and propionic acid production
Glycerol transformation
Glycerol hydrogenolysis
Glycerin
Lactic acid
Syngas
Rubber
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SCOPE BIOENERGY & SUSTAINABILITY
Land UseFeedstocks
Technologies
Impacts
Land use changes
Biomass potential
Biomass supply
Policy recommendations
Logistics
Much Needed Science
Certification
GHG emissions
Bioenergy numbers
Lignocellulosics
Costs
Financing
Food Security
Social aspects
Energy Security
Soil and water
Environmental Security and Climate Security
Biodiversity
Sustainable Development and Innovation
Rural development
Energy Access
Global assessment of Bioenergy & Sustainability:
FAPESP BIOEN, BIOTA and Climate Change Programs in collaboration with SCOPE
International Workshop: December 2-6, 2013, UNESCO, Paris
IUPAP Energy Committee
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BIOMASS
Contribute with knowledge and technologies for Sugarcane Improvement
Enable a Systems Biology approach for Biofuel Crops
Allelic variation for
breeding
Markers
SUCEST
Genes
ADRESSING THE SUGARCANE GENOME
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Genes
Regulatory regions
Regulatory sequences
Structural regions (centromere-telomeres)
Euchromatin & Heterochromatin
Repetitive sequences (Transposable elements, SSR)
Translational machinery (ribossomal genes, tRNAs)
sugarcane polyploid genome
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317 BAC sequenced
1,400 protein-coding genes
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Gene evolution
RPA1a
11
Gene regulation
RPA1a
12
Starch & Sucrose metabolism
β-D-fructose
Sucrose - 6P
EC 3.2.1.26
(2 reads)
EC 2.4.1.14
(174-341 reads)
EC 3.1.3.24
(109 reads)
EC 2.4.1.12
(6237 reads)
β-D-glucose
UDP glucose
SUCROSE
SUCROSE
EC 2.4.1.13
(1698 reads)
Cell WALL
Wall
CELL
components
PARTS
EC 3.2.1.37
(1894 reads)
D - xylose
EC 3.1.1.11
(24 reads)
Pectate
EC 3.2.1.15
(23 reads)
D - galacturonate
cellulose
CELLULOSE
EC 2.7.7.9
(2358 reads)
α-D-gluocse-1P
EC 5.4.2.2
(15413 reads)
α-D-gluocse-6P
EC 2.4.1.21
(547 reads)
Sh2
ADPglucose
Amylose
EC 2.4.1.18
EC 2.7.7.27
(3625 reads)
3 loci
1. Sb09g029610 (1295-5065 reads)
2. Sb01g008940 (443-501 reads)
STARCH
STARCH
3. Sb03g028850 (4-79 reads)
EC 3.2.1.2
(311 reads)
maltose
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Gene innovation
sugarcane hybrid (11)
S. spontaneum (2)
S. offcinarum (1)
Miscanthus sp. (1)
S. bicolor (genome)
single sequence
one substitution
SHCRBa_104_G22
0
1K
2K
3K
4K
5K
S6PP
6K
S6PP
2995F
4500R
3223F
3404F
4231R
4144R
3618R 3811F
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Initiatives around the globe:
SUGESI (Sugarcane Genome Sequencing Initiative) > Strategies
for Sequencing a Highly Complex Genome
1. BAC-sequencing
2. Shotgun sequencing
3. Methyl-filtration-NGS
4. 11,000 tilling BAC seq
5. Sequenom-SNP
6. Transcriptome initiatives
7. Spont & Off shotgun seq
8. Assembly algorithms
1,4
7
1,2,3,7,
8
1,2,3,5,6,7,8
1,4
2,6
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Integrating DATA around the globe: Euchromatic BAC
miRNA precursor
mapping
pathogen treatment
RNAseq mapping
Metilfiltrated gDNA
Other cultivars
gDNA
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BIOEN
Team
Van Sluys FW Munich 2014
Glaucia M Souza (BIOEN Pgm Coord)
GaTE Lab
Marcos Buckeridge (INCT coord)
Renato Vicentini (CBMEG- UNICAMP)
Fabio Nogueira (IB-UNESP Botucatu)
Michel Vincentz (CBMEG- UNICAMP)
Anete Prereira de Souza (CBMEG- UNICAMP)
Monalisa Sampaio (UFSCar – Breeding Pgm)
Antonio Augusto Garcia (ESALQ)
Helaine Carrer (ESALQ)
Maria Lucia Carneiro (ESALQ)
Daniel Scherer Moura (ESALQ)
Marcio Castro Silva Filho (ESALQ)
Cushla Metcalfe (PD FAPESP)
Guilherme M. Q. Cruz (DD FAPESP)
Edgar Andres Ochoa (DD CNPq)
Andreia Prata (DD FAPESP)
Tatiane Correa (TS-USP)
Jonas Gaiarsa (DD-FAPESP) – Infra Bioinf
Claudia Monteiro-Vitorello (ESALQ - Bioinf)
Joao Paulo Kitajima (IEAE-SP – Bioinf)
Nathalia de Setta (UFABC)
Partners
International
Angelique D’Hont (CIRAD, Fr)
Helene Bergers (CNRGV, Fr)
Ray Ming (U. Illinois, US)
A Paterson (U Georgia, US)
K Aitken (CSIRO-Aus)
Brasil
Katia C Scortecci (UFRN)
Douglas da Silva Domingues (IAPAR)
Financial Support
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The present release of sugarcane genomic sequences
will advance our understanding of sugarcane genetics
and contribute to the development of molecular tools
for breeding purposes and gene discovery. In paralell,
tools to assemble the sugarcane genome are being
developed.
Thank you!
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