CURRICULUM VITAE
Personal Details
Name:
Ana Lúcia Fernandes Pereira
e-mail:
[email protected]
Date of Birth:
25th November 1981
Employment
2010-…
Postdoctoral Fellow – Chromosome Instability & Dynamics Lab., Instituto de Biologia
Molecular e Celular (IBMC), Porto, Portugal
Major
Methods in Animal Experimentation (Mice)
techniques
Mammalian Cell Culture (cell lines, primary cells)
Brightfield and Confocal Microscopy
Animal Embryology and Physiology expertise
Animal Tissue Preparation for Microscopic Analysis
Education
2006-2010
Ph.D. in Human Biology – Faculdade de Medicina da Universidade do Porto, Porto, Portugal
2006-2009
Ph.D. student (January 2009-January 2010): Chromosome Instability & Dynamics Lab.,
Instituto de Biologia Molecular e Celular (IBMC), Porto, Portugal
Ph.D. student (February 2006 - December 2008): Department of Cell Biology, Erasmus
Medical Center, Rotterdam, The Netherlands
Project: “Generation and functional analysis of Clasp KO mice”
(Supervisor: Dr. Helder Maiato; Co-supervisor: Dr. Niels Galjart )
Major
Methods in Animal Experimentation (Mice)
techniques
Animal Physiology expertise
Mammalian cell culture (cell lines, primary cells), cell transfection, production of lentivirus,
cell transduction.
Molecular Biology Techniques: cloning, PCR, southern blot.
Biochemistry techniques: western blot, protein expression and purification.
2005
Undergraduate student/Fellowship: Chromosome Instability & Dynamics Lab., Instituto de
Biologia Molecular e Celular (IBMC), Porto, Portugal
Project: “Dissecção Molecular In Vivo das Vias Morfogenéticas do Fuso Mitótico –
Implicações para a Aneuploidia e o Cancro”.
(Supervisor: Dr. Helder Maiato)
Major
Cell
Biology
techniques:
techniques
immunofluorescence
mammalian
cell
culture
(cell
lines,
primary
cells),
Optical Microscopy: live-cell imaging, fluorescence microscopy.
2003-2004
Undergraduated internship: Instituto de Biologia Molecular e Celular (IBMC), Porto,
Portugal; Faculdade de Ciências da Universidade do Porto (FCUP), Poro, Portugal
Project: “Selecção de compostos com interesse terapêutico nas amiloidoses hereditárias
associadas à transtirretina (TTR) – estudo do efeito estabilizador no tetrâmero da TTR”.
Unidade de Neurobiologia Molecular (IBMC) and Departamento de Química da Faculdade de
Ciências (FCUP) (Supervisors: Doutora Rosário Almeida and Doutora Margarida Bastos)
Major
Biochemistry: production and purification of recombinant proteins, competition assays of a
techniques
radioactive compound, bi-dimensional analysis, isoelectric focusing
Methods of isoelectric calorimetry
1999-2004
Biochemistry degree (Licenciatura)
Faculdade de Ciências da Universidade do Porto (FCUP), Portugal / Instituto de Ciências
Biomédicas de Abel Salazar (ICBAS), Porto, Portugal
Certificates Obtained

Radiation Protection Course (level 5b) Erasmus Medical Centre, Rotterdam, The Netherlands, June 2005
(local certificate, giving the right to perform experiments with radioactivity at the Erasmus MC)

International Course on Laboratory Animal Science Department of Laboratory Animal Science,
Utrecht University, The Netherlands, June 2004 (“Artikel 9” certificate, giving the right to do experiments
with laboratory animals, equivalent to FELASA cat.C)
Communications
Poster presentations:

Ana Lúcia Pereira, Luísa Ferreira, Martine Jaegle, Robbert Rottier, Niels Galjart and Helder Maiato.
“CLASP1 is essential for neonatal lung function and survival”. I3S Scientific Retreat, Póvoa de Varzim,
Portugal, May 2014

Ana Lúcia Pereira, Luísa Ferreira, Martine Jaegle, Niels Galjart and Helder Maiato. “CLASP1 is essential
for neonatal lung fuction and survival”. ASCB Meeting, San Francisco, CA, USA, December 2012

Ana Lúcia Pereira, Luísa Ferreira, Martine Jaegle, Niels Galjart and Helder Maiato. “CLASP1 is essential
for neonatal lung fuction and survival”. I3S Scientific Retreat, Póvoa de Varzim, Portugal, May 2012

Ana Lúcia Pereira, Luísa Ferreira, Martine Jaegle, Niels Galjart and Helder Maiato. “CLASP1 is an
essential protein for life after birth”. I3S Scientific Retreat, Póvoa de Varzim, Portugal, May 2011

Ana Lúcia Pereira, Luísa Ferreira, Martine Jaegle, Niels Galjart and Helder Maiato. “CLASP1 is essential
for murine Viability”. Aneuploidy Meeting, Edinburgh, Scottland, June 2010

Ana Lúcia Pereira, Luísa Ferreira, Martine Jaegle, Niels Galjart and Helder Maiato. “CLASP1 is essential
for murine Viability”. I3S Scientific Retreat, Póvoa de Varzim, Portugal, May 2010

Ana L. Pereira, António J. Pereira, Ana R.R. Maia, Ksenija Drabek, C. Laura Sayas, Polla J. Hergerts,
Mariana Lince-Faria, Irina Matos, Cristina Duque, Tatiana Stepanova, Conly L. Rieders, William
Earnshaw, Niels Galjart and Helder Maiato. “Mammalian CLASP1 and CLASP2 Cooperate to Ensure
Mitotic Fidelity by Regulating Spindle and Kinetochore function”. ASCB Meeting, San Diogo,CA, USA,
December 2006
Oral communications (Invited Lecturer/Invited Speaker)

Master Course in Molecular and Cell Biology, "Cell Division: the role of CLASPs in mammalian
development and aneuploidy prevention”, Faculdade de Ciências da Universidade do Porto, (2015)

7th Course on Optical Microscopy Imaging for Biosciences, "Tissue preparation for Microscopic
Analysis", Porto, Portugal (2015)

6th Course on Optical Microscopy Imaging for Biosciences, "Tissue preparation for Microscopic
Analysis", Porto, Portugal (2014)

XVI Portugaliae Genetica, "From cells to animals: CLASPing the bigger picture", Porto, Portugal, 2014

Master Course in Molecular and Cell Biology, "Cell Division: the role of CLASPs in mammalian
development and aneuploidy prevention”, Faculdade de Ciências da Universidade do Porto, (2014)

Biochemistry Course, "CLASPs in Cell Division and Aneuploidy Prevention", Faculdade de Ciências da
Universidade do Porto, (2014)

Biology Course, "CLASPs in Cell Division and Aneuploidy Prevention", Faculdade de Ciências da
Universidade do Porto, (2014)

5th Course on Optical Microscopy Imaging for Biosciences, "Tissue preparation for Microscopic
Analysis", Porto, Portugal (2013)

Biology Course, "CLASPs in Cell Division and Aneuploidy Prevention", Faculdade de Ciências da
Universidade do Porto, (2013)

Biochemistry Course, "CLASPs in Cell Division and Aneuploidy Prevention", Faculdade de Ciências da
Universidade do Porto, (2013)

Postoc Seminar Series, "Clasp1 is essential for neonatal lung function and survival", Instituto de Biologia
Molecular e Celular, Porto, Portugal (2012)

Master Course in Molecular and Cell Biology, "Cell Division: the role of CLASPs in aneuploidy
prevention”, Faculdade de Ciências da Universidade do Porto, (2012)

Master Course in “Diagnóstico e Terapêutica” (Diagnosis and Therapeutics), "Afonso Clasp1: da geração à
análise funcional de um modelo knockout", Escola Superior de Tecnologia da Saúde do Porto, Portugal
(2012)
Conferences attended

18th MGC Symposium, Rotterdam, September 2008

European Career Event in Molecular Cell Biology and Medicine, June 2007, Porto, Portugal

17th MGC Symposium, Rotterdam, September 2007
Student Supervision

Co-Supervisor of Ana LuísaFerreira during her Master Course - Master in Tecnologia Bioquímica
em Saúde (Biochemical Technology in Health), Escola Superior de Tecnologia da Saúde do Porto,
Instituto Politécnico do Porto, Porto, Portugal)
Publications
Scientific Research
Microtubule detyrosination guides chromosomes during mitosis
Barisic M., Silva e Sousa R., Tripathy S.K., Magiera M.M., Zaytsev A.V., Pereira A.L., Janke C., Grishchuk E.L.,
Maiato H. (2015) Science. 15;348(6236):799-803
The microtubule plus-end tracking protein CLASP2 is required for hematopoiesis and hematopoietic stem
cell maintenance
Drabek K., Gutiérrez L., Vermeij M., Clapes T., Patel S.R., Boisset J.C., van Haren J., Pereira A.L., Liu Z., Akinci
U., Nikolic T., van Ijcken W., van den Hout M., Meinders M., Melo C., Sambade C., Drabek D., Hendriks R.W.,
Philipsen S., Mommaas M., Grosveld F., Maiato H., Italiano J.E. Jr, Robin C., Galjart N.(2012). Cell Rep.
2(4):781-8
Motor-independent targeting of CLASPs to kinetochores by CENP-E promotes microtubule turnover and
poleward flux
Maffini S., Maia A.R.R., Manning A.L., Maliga Z., Pereira A.L., Junqueira M., Shevchenko A. Hyman A., Yates
J.R. 3rd, Galjart N., Compton D.A. and Maiato H. (2009), Curr Biol 19(18):1566-72
Mammalian CLASP1 and CLASP2 Cooperate to Ensure Mitotic Fidelity by Regulating Spindle and
Kinetochore function
Ana L. Pereira, António J. Pereira, Ana R.R. Maia, Ksenija Drabek, C. Laura Sayas, Polla J. Hergerts, Mariana
Lince-Faria, Irina Matos, Cristina Duque, Tatiana Stepanova, Conly L. Rieders, William Earnshaw, Niels Galjart
and Helder Maiato (2006), Molecular Biology of the Cell 17, 4526-4542
New designs for MRI contrast agents
P.A. Fernandes, A.T.P. Carvalho, A.T. Marques, A.L.F. Pereira, A.P.S. Madeira, A.S.P. Ribeiro, A.F.R. Carvalho,
E.T.A. Ricardo, F.J.V. Pinto, H.A. Santos, H.D.G. Mangericão, H.M. Martins, H.D.B. Pinto, H.R.R. Santos, I.S.
Moreira, M.J.V. Azeredo, R.P.S. Abreu, R.M.S. Oliveira, S.F.M. Sousa, R.J.A.M. Silva, Z.S. Mourão & M.J.
Ramos (2003), J Comput Aided Mol Des 17, 463-473
Reviews and Book Chapters
Microtubule plus-end tracking proteins. eLS, DOI:10.1002/9780470015902.a0025979
Pereira, A.L. and Maiato,H.
Microtubule plus-end tracking proteins and their role in cell division
Ferreira, J.G., Pereira, A.L., Maiato, H.; Int Rev Cell Mol Biol. 2014;309:59-140.
Thesis
Generation and Functional Analysis of Clasp1 Knockout Mice – Ph.D. Thesis, 2009
Ana Lúcia Fernandes Pereira