Especificidade de enzimas de restrição
Eletroforese – padrão de digestão
Southern Blot
Southern Blot
Sequenciamento
Genoma completo
Em fase sólida
PCR
Estudos Forenses
Figure 6.10. DNA and Forensics. DNA analysis can be used to
establish guilt in criminal cases. Here, DNA was isolated from
bloodstains on the pants and shirt of a defendant and amplified
by PCR. The DNA was then compared to the DNA from the
victim as well as the defendant using gel electrophoresis and
autoradiography. DNA from the bloodstains on the defendant's
clothing matched the pattern of the victim, but not that of the
defendant. The frequency of a coincidental match of the DNA
pattern on the clothing and the victim is approximately 1 in 33
billion. Lanes λ, 1kb, and TS = Control DNA samples; lane D =
DNA from the defendant; jeans = DNA isolated from bloodstains
on defendent's pants; shirt = DNA isolated from bloodstains of
the defendant's shirt (two different amounts analyzed); V = DNA
sample from victim's blood.
Clonagem
Adaptadores
Plasmídios – mapa de restrição
Fagos como vetores de clonagem – empacotamento “in vitro”
M13 fago secretado
Biblioteca Genômica
Triagem
Sonda degenerada
YAC
BAC