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23 Congress of the International Union for Biochemistry and Molecular Biology
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44 Annual Meeting of the Brazilian Society for Biochemistry and Molecular Biology
th
th
Foz do Iguaçu, PR, Brazil, August 24 to 28 , 2015
CYTOTOXICITY EVALUATION OF PLANT SPECIES OF THE BRAZILIAN
NORTHEAST
Fonseca, A. G.1; Assis, C. S.¹; Vaz, E C. S.1; Fernandes, J. M.2 ; Souza Neto, M.
A.2; Langasner, S. Z.2 ; Cruz, A.K.M.³; Rocha, H.A.O.³; Ferreira, M. R. A.4; Souza,
J. A. L.4; Vigo, C. L. S.4; Soares, L. A. L. 4; Lemos, T. M. A. M.¹
¹ Departamento de Análises Clínicas e Toxicológicas, Universidade Federal do
Rio Grande do Norte, Natal, RN, Brazil.
² Departamento de Farmácia, Universidade Federal do Rio Grande do Norte,
Natal, RN, Brazil.
³ Departamento de Bioquímica, Universidade Federal do Rio Grande do Norte,
Natal, RN, Brazil.
4
Departamento de Ciências Farmacêuticas, Núcleo de Desenvolvimento Analítico
e Tecnológicos de Fitoterápicos, Universidade Federal de Permanbuco, Recife,
PE, Brazil.
Introduction and objectives: Brazil has a great biodiversity of plant species that
are popularly used for the treatment of various diseases. The Brazilian Northeast
is responsible for much of this use. Scientific research in this area promotes
greater information leading to the safe use. Therefore, the aim of this study was to
evaluate the cytotoxicity of popularly used plant species against normal and tumor
cell lines. Material and Methods: 3T3 (mouse fibroblast) and 786-0 (kidney
carcinoma) cell lines were exposed to extracts (from 0.1 to 100 µg/100µL) from
Kalanchoe brasiliensis (saião), Ziziphus joazeiro (juazeiro), Libidibia ferrea Martius
(jucá), Anacardium occidentale (cajú) and Schinus terebinthifolius Raddi (aroeira)
species for 24 hours and the cell viability was measured using MTT assay. The
absorbance of each well was measured at 570 nm. Results and conclusions:
Several samples stimulated the 3T3 cell growth, highlight A. occidentale (cajú)
effect, in the presence of this extract the cells growth 6-fold higher in comparison
with cell from control group. On the other hand, jucá, aroeira and joazeiro extracts
inhibits cells viability (30.98, 42.08 and 87.55% respectively). All extracts showed
inhibition of 786-0 cell line growth. The jucá and joazeiro extracts showed
respectively 47.22% and 90.56% inhibition at the dose of 100 µg/100µl. The most
potent cytotoxic extract was saião extract that inhibits 786-0 cells viability in a rate
of 94%. Extracts of jucá, joazeiro and saião proved to be good inhibitors of tumor
cells therefore more promising as future therapeutic agents.
Palavras-chave: Medicinal plants, cytotoxicity, kidney carcinoma cells.
Brazilian Society for Biochemistry and