ANTIOXIDANT AND ANTI-INFLAMMATORY PROFILE OF AMBURANIN A
AND AMBURANIN B, FLAVONOIDS ISOLATED FROM Amburana cearensis A
C SMITH (FABACEAE) IN HUMAN NEUTROPHILS.
LOPES, A.A.1 ([email protected]), SASAHARA, G.L.1, CANUTO, K.M.2, SILVEIRA, E. R.2, AZZOLINI,
A.E.S.C.3, LUCISANO-VALIM, Y.M.3, LEAL, L.K.A.M.1
1. Department of Pharmacy, Faculty of Pharmacy, Odontology and Nursing, Federal University of Ceará,
Fortaleza, Ceará, Brazil.
2. Departament of Organic and Inorganic Chemistry,Science Center, Federal University of Ceará,
Fortaleza, Ceará, Brazil.
3. Laboratory of Biochemistry, Departament of Chemistry and Physics, Faculty of Pharmaceutical
Sciences, São Paulo University, Ribeirão Preto, São Paulo, Brazil.
Amburana cearensis, neutrophils, inflammation
1. Introduction
Amburanin A (ABN A) and amburanin B (ABN
B) are compounds isolated from Amburana
cearensis A. C. Smith (Fabaceae), known
populary as cumaru, which has been used by
national
pharmaceutical
industries
and
government programs of phytotherapy in the
Northeast of Brazil. Several studies have been
showed that other compounds isolated from
Amburana cearensis have pharmalogical
activities, for example coumarin 1,amburoside
A2 and afrormosin3.
1.9, respectively) as same as ABN B (25, 50 and
100 µg/mL) (58.00 ± 0.55, 54.37 ± 1.02 and
51.59 ± 4.16, respectively) when compared to
control (57.92 ± 1.94). Both ABN A and ABN
B had anti-inflammatory effect by decreasing
levels of MPO release corresponding to
inhibitions up to 91 % and 90 %, respectively.
The ABN A and ABN B (6.25, 12.5, 25, 50 and
100 µg/mL) showed modulatory activity of
luminol- dependent (CL lum)
chemoluminescence production corresponding
to inhibitions up to 94% and 95%, respectively.
2. Methods
Polimorphonuclear cells (2,5 x 106 cells/mL),
predominantly neutrophils (80 – 90%) were
isolated and incubated with ABN A or ABN B
(25-100 µg/mL), vehicle (Tween 0,4%, control),
HBSS (non-treated cells) or Triton X 100 (2 %,
cytotoxic strandard) and the cytotoxic tests were
measured by lactate dehydrogenase activity.
The anti-inflammatory effect was investigated
by inhibition of neutrophil degranulation
induced by phorbol mirystate acetate (PMA; 0.1
µM) where neutrophis were incubated with
ABN A or ABN B (25-100 µg/mL), HBSS or
vehicle (Tween 0.4%, control) and the levels of
myeloperoxidase (MPO, inflammation marker)
were determinated at cells supernadants. Free
radicals were triggered by addition of PMA (0.1
µM) and antioxidant activity was determinated
by chemoluminescence luminol-dependent. The
results were expressed by mean ± standard
deviation (cytotoxic evaluation) or percentage
of inhibition (anti-inflammatory and antioxidant
activities).
4. Conclusion
The flavonoids amburanin A and amburanin B
have an antiiflammatory activity by inhibition
of neutrophil degranulation by decrease of
myeloperoxidase levels (a biomarker of
inflammation) and antioxidant activity
modulating mainly products related with
NADPH oxidase activity, as myeloperoxidase.
Acknowledgments: CAPES
1
Leal, L. K. A.M.; Matos, F. J. A.; Viana, G.S.B.
Antinociceptive and antiedematogenic effects of the
hydroalcoholic extract and coumarin from Torresea
cearensis Fr. All. Phytomedicine. v. 04, p. 221–7, 1997.
2
Leal, L. K.; Canuto, K. M.; da Silva Costa, K. C.; NobreJúnior, H. V.; Vasconcelos, S.M.; Silveira, E. R.; Ferreira,
M. V.; Fontenele, J. B.; Andrade, G. M.; de Barros Viana,
G. S. Effects of amburoside A and isokaempferide,
polyphenols from Amburana cearensis, on rodent
inflammatory processes and myeloperoxidase activity in
human neutrophils. Basic Clin Pharmacol Toxicol. v. 104,
p. 198-205, 2009.
3
3. Results
The results showed that ABN A (25, 50 and 100
µg/mL) did not showed cytotoxic effect by LDH
activity (48.48 ± 0.82, 50.25 ± 1.33 and 47.50 ±
Lopes, A. A. Avaliação da atividade anti-inflamatória e
antioxidante das cápsulas do extrato seco e da afrormosina,
obtidos de Amburana cearenses A C Smith. 2010.
Dissertação de mestrado – Departamento de Fisiologia e
Farmacologia, Universidade Federal do Ceará, Fortaleza.