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23 Congress of the International Union for Biochemistry and Molecular Biology
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44 Annual Meeting of the Brazilian Society for Biochemistry and Molecular Biology
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Foz do Iguaçu, PR, Brazil, August 24 to 28 , 2015
ARAÇÁ (Psidium cattleianum Sabine) EXTRACT: CHARACTERIZATION AS
PRIOR ANALYSIS FOR ANTIOXIDANT CAPACITY ASSESSMENT
Moraes, L.S.S.1; Vasconcellos, M.M.1; Denardin, C.C.1; Avila, D.S.1
1
Grupo de Pesquisa em Bioquímica e Toxicologia em Caenorhabdits elegans –
GBToxCe, Universidade Federal do Pampa, Rio Grande do Sul, Brasil.
Araçá (Psidium cattleianum Sabine) is an edible fruit, belonging to Myrtaceae
family, native to Rio Grande do Sul (Brazil), which has two varieties: yellow and
red. The aim of this study was to characterize the extracts of yellow and red araçá
through the determination of total phenolics, flavonoids and carotenoids,
antioxidant activity and toxicity using the nematode Caenorhabditis elegans. The
fruits were obtained from Embrapa Temperate Climate, Pelotas/RS - Brazil, and
the extracts were prepared by ethanol extraction (95ºGL), with evaporation in
rotary evaporator. The determination of phenolic compounds (Folin-Ciocalteu
method/CAE - chlorogenic acid equivalent), flavonoid total content (colorimetric
method with aluminum chloride/QE - quercetin equivalents), carotenoids total
content (extraction with ethyl acetate/petroleum ether) and antioxidant capacity
(DPPH radical) were performed in the extracts. The toxicity was tested in C.
elegans, in concentrations of 500µg/mL, 1000µg/mL, 2000µg/mL e 5000µg/mL
(worms at L1 larval stage, pre-treated for 30min, and counting after 24 hours to
evaluate the amount of live worms). The concentrations of phenolic compounds,
for both extracts were similar, 67.693,29µg CAE/mL and 61.323,26µg CAE/mL, for
yellow and red araçá, respectively; while the flavonoids found 263.87mg of
QE/100g of fruit in yellow and 149.71mg of QE/100g of fruit in red. The
carotenoids content were 19.68 and 35.94 µg ß caroteno/mL extract to yellow and
red, respectively. We observed that the antioxidant capacity of yellow extract was
more antioxidant (EC50 = 68.63g/L) than the red extract (EC50 = 106.61g/L). In a
toxicity test, for both extracts, any of concentrations tested caused death in worms,
and is visible a greater amount of worms in plates with extract compared to
control. Based in this data both extracts have antioxidant properties because the
amount of phenolic compounds, flavonoids and carotenoids, and can be observed
that showed no toxic effects up to a concentration of 5000µg/mL.
Keywords: araçá, phenolic compounds, flavonoids, antioxidant.
References:
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vegetables. Journal of the University of Chemical Technology and Metallurgy.
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MEDINA, A.L. et al. Araça (Psidium cattleianum Sabine) fruit extracts with
antioxidant and antimicrobial activities and antiproliferative effect on human cancer
cells. Food Chemistry. 128:916–922, 2011.
RODRIGUEZ-AMAYA, D. B. A guide to carotenoids analysis in foods.
Washington: Ilsi, 1999. 119p
Brazilian Society for Biochemistry and
rd
23 Congress of the International Union for Biochemistry and Molecular Biology
th
44 Annual Meeting of the Brazilian Society for Biochemistry and Molecular Biology
th
th
Foz do Iguaçu, PR, Brazil, August 24 to 28 , 2015
RUFINO, M.S.M., et al. Metodologia científica: determinação da atividade
antioxidante total em frutas pela captura do radical DPPH. Embrapa Agroindústria
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Brazilian Society for Biochemistry and