Macrophage-Mediated Phagocytosis of
Bacteria Adhering on Biomaterial
Surfaces
Joana Filipa da Silva Domingues
Macrophage-mediated phagocytosis of bacteria adhering on biomaterial surfaces
By Joana Filipa da Silva Domingues
University Medical Center Groningen, University of Groningen
Groningen, The Netherlands
Cover design by José Tiago da Silva Domingues
Copyright © 2014 by Joana Filipa da Silva Domingues
Printed by Gildeprint Drukkerijen
ISBN: 978-94-6108-670-9
Macrophage-Mediated Phagocytosis of
Bacteria Adhering on Biomaterial
Surfaces
Proefschrift
ter verkrijging van de graad van doctor aan de
Rijksuniversiteit Groningen
op gezag van de
rector magnificus prof. dr. E. Sterken
en volgens besluit van het College voor Promoties.
De openbare verdediging zal plaatsvinden op
woensdag 28 mei 2014 om 12.45 uur
door
Joana Filipa da Silva Domingues
geboren op 30 juni 1984
te Braga, Portugal
Promotores
Prof. dr. H. C. van der Mei
Prof. dr. ir. H. J. Busscher
Copromotor
Dr. T. G. van Kooten
Beoordelingscommissie
Prof. dr. R. A. Bank
Prof. dr. L. H. Koole
Prof. dr. R. L. Reis
Paranimfen
Raquel da Cruz Barros
Chongxia Yue
Dedicado à minha família.
Table of Contents
Chapter I
General Introduction: Bridging the gap between in
vitro and in vivo evaluation of biomaterial-associated
infections.
1
Chapter II
Macrophage response to staphylococcal biofilms on
crosslinked poly(ethylene) glycol polymer coatings
and common biomaterials in vitro.
19
Chapter III
Phagocytosis of bacteria adhering to a biomaterial
surface in a surface thermodynamic perspective.
37
Chapter IV
Superparamagnetic iron oxide nanoparticles
stimulate macrophages in removing biofilms.
69
Chapter V
In vitro interactions between bacteria, osteoblast-like
cells and macrophages in the pathogenesis of
biomaterial-associated infections.
91
Chapter VI
Adhesive patches in a poly(ethylene glycol)-matrix
reduce bacterial adhesion, while enhancing tissue cell
adhesion and macrophage phagocytic activity.
115
Chapter VII
General Discussion
139
Summary
149
Sumário
155
Samenvatting
163
Acknowledgements
169