Abbreviated Curriculum Vitae
Name: Pedro Manuel H. M. Matias
Official address: Instituto de Tecnologia Química e Biológica António Xavier, Av. República,
EAN, 2784-505 OEIRAS, Portugal
Phone: +351-21-446-9669 Fax: +351-21-443-3644 e-mail: [email protected]
Date and place of birth: Lisbon, March 5, 1959
Nationality: Portuguese
Education:
1983 - Dipl. in Chemical Engineering, IST, Lisbon, Portugal
1986 - Ph.D. in Crystallography, University of Pittsburgh, Pittsburgh, USA
Academic career:
Assistant Professor, IST, Lisbon, 1990-1992
Research Assistant, ITQB, Oeiras, 1992-1995
Senior Research Assistant, ITQB, Oeiras, 1995-2008
Principal Researcher, ITQB, Oeiras, 2008-present
Research Career:
Member of the Crystallography Group at Centro de Química Estrutural (IST, Lisbon) from
1990 to 1992
Member of the Protein Crystallography Laboratory at ITQB from 1990 to 2006
Head of the Industrial and Medical Applications Crystallography Laboratory at ITQB since
2007
Research Interests:
Structural studies of proteins and protein complexes with human health implications.
Structural studies of electron transfer proteins, metalloproteins and metalloenzymes.
Application of methods involving data obtained in synchrotrons, namely Multi-wavelength
Anomalous Dispersion.
Application of in-house measurements of sulfur anomalous dispersion signal to the
determination of three-dimensional structures of macromolecules.
Research-related Activities
Coordinator of the Portuguese Beam Allocation Group for Protein Crystallography at ESRF
(1999-present).
Member of the ESRF Users Organization Committee (2003-2006).
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Member of the ESRF Review Panel for Macromolecular Crystallography (2003-2005).
Chairperson of the ESRF BM14 CRG Review Panel (2010-2012).
Research Grants as PI:
PTDC/BBB-BEP/2885/2014 (2016-2018) - Engineering a highly active NiFeSe Hydrogenase
for electrocatalytic and photocatalytic applications
PTDC/BBB-BEP/0934/2012 (2013-2015) - Structural Determinants of Oxygen Tolerance in a
NiFeSe Hydrogenase
FCT PDCT/BIA-PRO/70429/2006 (2008-2012) - Towards biohydrogen production – study of a
high-activity, oxygen-resistant bacterial hydrogenase
FCT PDCT/QUI/71142/2006 (2008-2012) - Molecular Adaptation to Extreme Environments:
Structural Studies of Proteins Involved in the Synthesis of Osmolytes in (hyper)thermophylic
micro-organisms
PRAXIS PCNA/P/BIO/47/96 (1996-1999)
JNICT PMCT/C/BIO 874/90 (1990-1993)
Research Grants as Participant:
PTDC/BBB-BEP/1463/2014 (2016-2018) – Structure and function of a molecular machine:
the RuvBL1/RuvBL2 complex and its role in disease
PTDC/BBB-BEP/1724/2012 (2013-2015) - Structural and functional insights into centriole
biogenesis
PTDC/BIA-PRO/111940/2009 (2011-2014) - Structural determinants of superoxide reduction
- A detoxification system essential for life
PTDC/BIA-PRO/098158/2008 (2010-2013) - Mind the gap: How extracellular respiration is
linked across the periplasmic space to the cytoplasmic oxidation of substrates. A key step in
bioenergy harvesting
PTDC/QUI-BIQ/098406/2008 (2010-2013) - Engineering mini Superoxide Dismutases with
tunable redox properties
PTDC/BIA-PRO/67107/2006 (2008-2011) - Iron metabolism in sulphate reducing bacteria
PTDC/BIA-PRO/67240/2006 (2008-2010) - Iron Metabolism Study in Deinococcus
radiodurans- Novel strategies
POCTI/BME/38306/2001
SPINE QLRT-2001-00988
MAX-INF HRPI-2000-40035 (2000-2004)
FCT-PRAXIS XXI-Agência de Inovação P003-P3IB-02/97 (1997-2000)
FCT-PRAXIS XXI-Agência de Inovação SASTEREC EUREKA 1048 (1997-2000)
FCT-PRAXIS XXI-PRAXIS/2/2.1/17/94 (1995-1999)
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EUROPEAN COMMISSION CHRX/CT93/0143 (1993-1997)
Teaching-Related Activities:
Member of the Scientific Commission of the Master in Biochemistry for Health Program
Participation in the ITQB Ph.D. Program in Molecular BioSciences
Participation in Courses and Workshops organized at ITQB by the Macromolecular
Crystallography Unit
Supervision of Ph.D. Students
Ana Maria de Jesus Bispo Varela Coelho (co-supervisor with Prof. Maria Arménia Carrondo)
Isabel Maria Travassos Bento (co-supervisor with Prof. Maria Arménia Carrondo)
Susana Margarida Pires Gonçalves
Marta Coimbra Marques (co-supervisor with Dr. Inês Cardoso Pereira).
Sara Teresa Neves da Silva (co-supervisor with com Dr. Tiago Bandeiras).
Sónia Alexandra dos Santos Zacarias (co-supervisor with Dr. Inês Cardoso Pereira).
Paulo Filipe Ribeiro do Espirito Santo (co-supervisor with com Dr. Tiago Bandeiras).
Participation in Ph.D. Examination juries
Ana Maria de Jesus Bispo Varela Coelho – Évora, March 1998 (main opponent).
Margarida Archer Franco Frazão – Oeiras, November 1998.
João Miguel Carrapa Nunes Dias – Monte da Caparica, June 2002 (main opponent).
Sofia Isabel Vaz Queiroz de Macedo – Oeiras, Novembro de 2002.
Carlos Manuel Alves da Costa Cunha – Monte da Caparica, November 2003 (main opponent).
Vitor Hugo Oliveira Teixeira – Oeiras, January 2007.
Ana Filipa de Matos Almeida Valente – Oeiras, April 2007 (main opponent).
Maxime Cuypers – Oeiras, Setembro de 2007 (main opponent).
João Vieira Rodrigues – Oeiras, Setembro de 2007.
Jadwiga Tatur – Delft, Novembro de 2007 (Adviseur).
M. G. Pompidor – Grenoble, Dezembro de 2007 (Rapporteur).
Filipe Folgosa – Monte da Caparica, Outubro de 2008
Susana Margarida Pires Gonçalves - Oeiras, Maio de 2012 (supervisor).
Lic. Fábio de Oliveira Morais e Silva - Oeiras, Dezembro de 2013.
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Lic.ª Marta Coimbra Marques - Oeiras, Dezembro de 2014 (co-supervisor).
Publications:
Number of publications in peer-reviewed journals: 87 since 1983
Number of communications presented at scientific meetings: 34 since 1982
Number of book chapters: 4 since 2001
Recent Articles (last 5 years):
1. C.M. Sousa, P. Carpentier, P.M. Matias, F. Testa, F. Pinho, P. Sarti, A. Giuffrè, T.M.
Bandeiras, C.V. Romão “Superoxide reductase from Giardia intestinalis: structural
characterization of the first SOR from a eukaryotic organism shows an iron centre highly
sensitive to photo-reduction” (2015) Acta Crystallographica D, accepted.
2. N. Zaarur, X. Xu, P. Lestienne, A.B. Meriin, M. McComb, C.E. Costello, G.P. Newnam, R.
Ganti, N.V. Romanova, M. Shanmugasundaram, S.T. Silva, T.M. Bandeiras, P.M. Matias,
K.S. Lobachev, I.K. Lednev, Y.O. Chernoff, M.Y. Sherman “RuvbL1 and RuvbL2 enhance
aggresome formation and disaggregate amyloid fibrils” (2015) EMBO J. 112(37):1145511460. http://dx.doi.org/10.15252/embj.201591245.
3. P.M. Matias, S.H. Baek, T.M. Bandeiras, A. Dutta, W.A. Houry, O. Llorca, J. Rosenbaum
“The AAA+ proteins Pontin and Reptin enter adult age: From understanding their basic
biology to the identification of selective inhibitors” (2015) Frontiers in Molecular
Biosciences 2:17. http://dx.doi.org/10.3389/fmolb.2015.00017
4. J.P. Leite, M. Duarte, A.M. Paiva, F. Ferreira-da-Silva, P.M. Matias, O.C. Nunes, L. Gales
“Structure-guided engineering of molinate hydrolase for the degradation of
thiocarbamate pesticides” (2015) PLoS One 10:e0123430.
http://dx.doi.org/10.1371/journal.pone.0123430
5. N. Borges, C. Jorge, L. Gonçalves, S. Gonçalves, P. Matias and H. Santos,
"Mannosylglycerate: structural analysis of biosynthesis and evolutionary history" (2014)
Extremophiles 18:835-852. http://dx.doi.org/10.1007/s00792-014-0661-x
6. S. P. Miller, S. Gonçalves, P. M. Matias, and A. M. Dean, "Evolution of a Transition State:
Role of Lys100 in the Active Site of Isocitrate Dehydrogenase" (2014) ChemBioChem,
15:1145-1153. http://dx.doi.org/10.1002/cbic.201400040
7. P. Nogly, P. M. Matias, M. de Rosa, R. Castro, H. Santos, A. R. Neves and M. Archer,
"High-resolution structure of an atypical -phosphoglucomutase related to eukaryotic
phosphomannomutases" (2013) Acta Crystallogr. D 69:2008:2016.
http://dx.doi.org/10.1107/S0907444913017046
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8. M. C. Marques, R. Coelho, I. A. C. Pereira and P. M. Matias, "Redox state-dependent
changes in the crystal structure of [NiFeSe] hydrogenase from Desulfovibrio vulgaris
Hildenborough" (2013) Int. J. Hydrogen Energ. 38(21):8664–8682.
http://dx.doi.org/10.1016/j.ijhydene.2013.04.132
9. J. Rosenbaum, S. H. Baek, A. Dutta, W. A. Houry, O. Huber, T. R. Hupp, P. M. Matias,
"The Emergence of the Conserved AAA+ ATPases Pontin and Reptin on the Signaling
Landscape" (2013) Sci. Signal. 6:mr1. http://dx.doi.org/10.1126/scisignal.2003906
10. S. Gonçalves, S. P. Miller, M. A. Carrondo, A. M. Dean and P. M. Matias, "Induced Fit and
the Catalytic Mechanism of Isocitrate Dehydrogenase" (2012) Biochemistry, 51:7098–
7115. http://dx.doi.org/10.1021/bi300483w
11. S. Gonçalves, A. M. Esteves, H. Santos, N. Borges and P. M. Matias, "The threedimensional structure of mannosyl-3-phosphoglycerate phosphatase from Thermus
thermophilus HB27: a new member of the Haloalcanoic Acid Dehalogenase Superfamily"
(2011) Biochemistry 50:9551–9567. http://dx.doi.org/10.1021/bi201171h.
12. S. Gorynia, T. M. Bandeiras, F. G. Pinho, C. E. McVey, C. Vonrhein, A. Round, D. I.
Svergun, P. Donner, P. M. Matias and M. A. Carrondo, "Structural and functional insights
into a dodecameric molecular machine – The RuvBL1/RuvBL2 complex" (2011) J. Struct.
Biol., 176:279-291. http://dx.doi.org/10.1016/j.jsb.2011.09.001.
13. F. G. Pinho, A. F. Pinto, L. C. Pinto, H. Huber, C. V. Romão, M. Teixeira, P. M. Matias and
T. M. Bandeiras, "Superoxide reductase from Nanoarchaeum equitans: expression,
purification, crystallization and preliminary X-ray crystallographic analysis" (2011) Acta
Cryst. F67:591-595. http://dx.doi.org/10.1107/S1744309111009432.
14. S. Gonçalves, A. M. Esteves, N. Borges, H. Santos and P. M. Matias, "Crystallization and
preliminary X-ray analysis of mannosyl-3-phosphoglycerate phosphatase from Thermus
thermophilus HB27" (2011) Acta Cryst. F67:390-396.
http://dx.doi.org/10.1107/S1744309111002843.
15. C. S. A. Baltazar, M. C. Marques, C. M. Soares, A. M. DeLacey, I. A. C. Pereira and P. M.
Matias, "Nickel-Iron-Selenium Hydrogenases - An Overview" (2011) Eur. J. Inorg.
Chem., 2011(7):948-962. http://dx.doi.org/10.1002/ejic.201001127
16. C. V. Romão, D. Ladakis, S. A. L. Lobo, M. A. Carrondo, A. A. Brindley, E. Deery, P. M.
Matias, R. W. Pickersgill, L. M. Saraiva and Martin J. Warren, "Evolution in a family of
chelatases facilitated by the introduction of active site asymmetry and protein
oligomerization" (2011) PNAS 108(1):97-102. DOI:10.1073/pnas.1014298108.
A full publication list can be found at: http://www.researcherid.com/rid/B-7180-2008
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Recent Oral Communications (last 5 years):
1. "A structural Overview of Pontin (RuvBL1), Reptin (RuvBL2) and their complex(es)",
PSDI 2014 – 22nd Protein Structure Determination in Industry, 3 a 5 de Novembro de
2014, Cascais, Portugal.
2. "Redox
State-Dependent
Structural
Changes
in
[NiFeSe]
Hydrogenase
from
Desulfovibrio vulgaris Hildenborough", 10th International Hydrogenase Conference, 8
a 12 de Julho de 2013, Szeged, Hungria.
3. "The Portuguese MX Block Allocation Group at the ESRF: a 14-year success history
(1999-2013)", 2º Encontro Nacional de Utilizadores de Radiação Sincrotrão, 14-15 de
Fevereiro de 2013, Lisboa, Portugal.
4. "A structural Overview of Pontin (RuvBL1), Reptin (RuvBL2) and their complex(es)",
First International Meeting on Reptin and Pontin, 16-18 October 2012, Bordeaux,
France.
5. "The Three Dimensional Structure of a Dodecameric Molecular Machine – The
RuvBL1/RuvBL2 Complex", INTERBIO Symposium Frontiers in Protein Research, 5-7
May 2011, Oeiras, Portugal.
Recent Book Chapters (last 5 years):
1. C. V. Romão, M. Archer, S. Lobo, R. O. Louro, I. A. C. Pereira, L. M. Saraiva,M. Teixeira
and P. M. Matias (2012) "Diversity of Heme Proteins in Sulfate Reducing Bacteria" in
HANDBOOK OF PORPHYRIN SCIENCE - With Applications to Chemistry, Physics,
Materials Science, Engineering, Biology and Medicine, Edited by K. M. Kadish, K. M.
Smith and R. Guilard, World Scientific Publishing Co., Pte. Ltd., Singapore, 19 (89) pp
139-230.
2. S. Gorynia, T. M. Bandeiras, P. M. Matias, F. G. Pinho, C. E. McVey, P. Donner and M.
A. Carrondo, "RuvBL1 and RuvBL2 and Their Complex Proteins Implicated in Many
Cellular Pathways" (2012) in
Macromolecular Crystallography, Edited by M. A.
Carrondo and P. Spadon, Springer, Dordrecht, The Netherlands, pp 55-63.
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