3rd Scientific Meeting of Institute for Biotechnology and Bioengineering Instituto Superior Técnico, Lisboa - 16th and 17th March 2012 Sessions: IBB Research Areas Friday March 16 th Saturday March 17 Industrial and Environmental Biotechnology Grande Anfiteatro IEB 11 IEB 12 IEB 13 IEB 14 09h00 09h20 09h40 10h00 COFFEE BREAK 10h50 11h10 11h30 11h50 IEB 15 IEB 16 IEB 17 IEB 18 th Plant, Animal and Food Biotechnology Sala 02.1 Health Biotechnology Sala 02.2 PAFB 11 PAFB 12 PAFB 13 PAFB 14 HB 11 HB 12 HB 13 HB 14 PAFB 15 PAFB 16 PAFB 17 PAFB 18 HB 15 HB 16 HB 17 HB 18 Marine Biotechnology Sala 02.1 Nanobiotechnology Sala 02.2 MB 1 MB 2 NB 1 NB 2 MB 3 NB 3 LUNCH 13h40 14h00 14h30 14h50 15h10 15h30 15h50 COFFEE BREAK 16h40 17h00 17h20 17h40 18h00 18h20 Opening Session – Grande Anfiteatro Industrial and Plant, Animal Health Environmental and Food Biotechnology Biotechnology Biotechnology Sala 02.2 Grande Anfiteatro Sala 02.1 IEB 1 PAFB 1 HB 1 IEB 2 PAFB 2 HB 2 IEB 3 PAFB 3 HB 3 IEB 4 PAFB 4 HB 4 IEB 5 PAFB 5 HB 5 IEB 6 PAFB 6 HB 6 IEB 7 PAFB 7 HB 7 IEB 8 PAFB 8 HB 8 IEB 9 PAFB 9 HB 9 IEB 10 PAFB 10 HB 10 Meeting of PIs of Research Units and Groups and International Advisory Board – Sala 02.1 • Industrial and Environmental Biotechnology (IEB) • Health Biotechnology (HB) • Plant, Animal and Food Biotechnology (PAFB) • Nanobiotechnology (NB) • Systems and Synthetic Biology (SSB) • Marine Biotechnology (MB) Systems and Synthetic Biology Grande Anfiteatro SSB 1 SSB 2 SSB 3 SSB 4 SSB 5 SSB 6 SSB 7 General Discussion and Closing Session Anfiteatro NB 4 NB 5 NB 6 NB 7 – Grande Contents ORAL COMMUNICATIONS: INDUSTRIAL AND ENVIRONMENTAL BIOTECHNOLOGY IEB 1 - FRANCISCO B. PEREIRA, PEDRO M.R. GUIMARÃES, DANIEL G. GOMES, NUNO P. MIRA, MIGUEL C. TEIXEIRA, ISABEL SÁ-CORREIA, LUCÍLIA DOMINGUES, Identification of Saccharomyces cerevisiae genes involved in the resistance to multiple stresses during Very-HighGravity and lignocellulosic biomass industrial fermentations IEB 2 - EDUARDO J. GUDIÑA, LÍGIA R. RODRIGUES, JOSÉ A. TEIXEIRA, Biosurfactant-producing Bacillus subtilis strains isolated from crude oil samples enhance oil recovery at lab scale IEB 3 - PEDRO FERNANDES, MARCO P.C. MARQUES, CARLA C.C.R. DE CARVALHO, JOAQUIM M.S. CABRAL, The role of microreactors in the development of bioconversion processes IEB 4 - I. BELO, M. LOPES, M. MOT, Application of hyperbaric bioreactors for bioprocess development IEB 5 - JOÃO M.B.T. CAVALHEIRO, RAPHÄEL CANADAS, RODRIGO S. RAPOSO, M. CATARINA M.D. DE ALMEIDA, M. TERESA CESÁRIO, Frederico C. Ferreira, M. Manuela R. da Fonseca, From waste glycerol to biocompatible PHA scaffolds IEB 6 - DANIEL F. SARAIVA, FILIPE G. FREIRE, RENATO CARVALHO, Triphasic Reactor Development for Soybean Oil Hydrogenation for Biodiesel Production IEB 7 - L. DOMINGUES, C.C. PINHEIRO, N.M.C. OLIVEIRA, A. VILELAS, J. FERNANDES, F. RAMÔA RIBEIRO, Model development and validation of ethyl tert-butyl ether (ETBE) production reactors using industrial plant data IEB 8 - RUTE CAETANO, MARIA AMÉLIA LEMOS, FRANCISCO LEMOS, FILIPE FREIRE, The Hydrogenation of Nitrobenzene to Aniline: Impact of Homogeneous Reactions IEB 9 - B. SILVA, H. FIGUEIREDO, O.S.G.P. SOARES, M.F.R. PEREIRA, J.L. FIGUEIREDO, A.E. LEWANDOWSKA, M.A. BAÑARES, I.C. NEVES, T. TAVARES, Evaluation of ion exchange-modified Y and ZSM5 zeolites in Cr(VI) biosorption and catalytic oxidation of ethyl acetate IEB 10 - A. NETO, S.THOMAS, G. BOND, F.THIBAULT-STARZYK, M.F.RIBEIRO, C.HENRIQUES, Oil shale transformation under microwave irradiation in the presence of water vapor or BEA zeolite IEB 11 - S. BETTENCOURT, A.R. BORBA, D. MENDONÇA, M.S. LOPES, N. SIMÕES, A. DA CÂMARA MACHADO, Plants of Azorean Hot Springs: an unexplored source of thermostable molecules IEB 12 - R.N.L. CARVALHO, N.M.T. LOURENÇO, L.P. FONSECA, Swelling behaviour of gelatin-ionic liquid functional polymers IEB 13 - L. BORLIDO, A.M. AZEVEDO, A.C.A. ROQUE, M.R. AIRES-BARROS, Affinity based purification of human monoclonal antibodies from CHO cell supernatants using boronic acid magnetic particles IEB 14 - M. OLIVEIRA, A.V. MACHADO, A.G. BRITO, R. NOGUEIRA, Phosphorus removal from eutrophic waters with a polymer nanocomposite containing aluminium IEB 15 - VÂNIA FERREIRA, LILIANA SANTOS, MARTA NETO, JOSÉ FERNANDES, MARIA ALCINA PEREIRA, ANÁLIA LOURENÇO, MANUEL MOTA, NELSON LIMA, ANA NICOLAU, New insights on the roles of bacteria and protozoa in activated-sludge processes IEB 16 - NÍDIA DANA LOURENÇO, ANA M.T. MATA, HELENA MARIA PINHEIRO, Aerobic granular sludge toward effective textile wastewater treatment IEB 17 - M.A. PEREIRA, A.J. CAVALEIRO, A.A. ABREU, J.C. COSTA, D.Z. SOUSA, M.M. ALVES, Sustainable Bioenergy Carriers from Wastes IEB 18 - RITA RIBEIRO, CARLA I.C. PINHEIRO, HELENA MARIA PINHEIRO, MARIA DO CÉU ALMEIDA, Monitoring and efficiency control of wastewater treatment adapted to small decentralized treatment plants ORAL COMMUNICATIONS: HEALTH BIOTECHNOLOGY HB 1 - FERNANDO DOURADO, MIGUEL GAMA, Bacterial Nano Cellulose - innovative Biopolymer in Research and Application HB 2 - SÓNIA SILVA, MELYSSA NEGRI, DOUGLAS MONTEIRO, MARIANA HENRIQUES, ROSÁRIO OLIVEIRA, JOANA AZEREDO, Virulence factors of non-Candida albicans Candida species HB 3 - DALILA MIL-HOMENS, ARSÉNIO M. FIALHO, Trimeric autotransporter adhesins in the human pathogen Burkholderia cenocepacia: a multifunctional family of proteins implicated in virulence HB 4 - C. ALMEIDA, L. CERQUEIRA, N.F. AZEVEDO, M.J. VIEIRA, Helicobacter pylori: role of water on survival and transmission HB 5 - ANA MARGARIDA SOUSA, ANA NICOLAU, ANÁLIA LOURENÇO, MARIA OLIVIA PEREIRA, Profiling of pathogenic bacteria by colony morphology – identification of potential biofilm resistance and virulence determinants HB 6 - WOLFGANG LINK, Protein localization in disease and therapy HB 7 - ÁLVARO TAVARES, Cell cycle control and the finding of tumor reversal drugs HB 8 - P.H. OLIVEIRA, C. LOBATO DA SILVA, J.M.S. CABRAL, An Appraisal of Human mtDNA Instability: Role of DNA Topology and Sequence Motifs HB 9 - ANA-TERESA MAIA, R. RUSSELL, M. O’REILLY, M. DUNNING, C. CALDAS, B.A.J. PONDER, Differential Allelic Expression In Breast Cancer Susceptibility HB 10 - CAROLINE J. SHEEBA, RAQUEL P. ANDRADE, ISABEL PALMEIRIM, Temporal and Spatial Integration of FGF & SHH Signaling Underlies Dynamic HES Expression in Developing Chick Limb HB 11 - JOÃO FACUCHO-OLIVEIRA, MARGARET BENTO, JOÃO FURTADO, ELISABETH CORREIA, TIAGO JUSTO, RITA DRAGO, JOSÉ MANUEL INÁCIO, JOSÉ ANTÓNIO BELO, Integrative studies on the role of Ccbe1 in cardiogenesis: from the embryo to ES cell derived cardiac tissue HB 12 - GUSTAVO TISCORNIA, ERICA LORENZO VIVAS, INA BERNIAKOVICH, LESLIE MATALONGA, CRISTINA EGUIZÁBAL ARGAIZ, FEDERICO GONZÁLEZ, RITA VASSENA, MONSERRAT BARRAGÁN MONASTERIO, LAURA GORT, ANTONIA RIBES, CARMEN ORTIZ MELLET, JOSÉ M. GARCÍA FERNANDEZ, ANNA VEIGA, JUAN CARLOS IZPISÚA BELMONTE, Modelling Gaucher’s Type II Disease Using Induced Pluripotent Stem Cells HB 13 - HÉLDER S.C. BARBOSA, TIAGO G. FERNANDES, TIAGO P. DIAS, MARIA MARGARIDA DIOGO, JOAQUIM M.S. CABRAL, Multifactorial Analysis of Embryonic Stem Cell Self-Renewal Reveals a Crucial Role of GSK-3-mediated Signaling under Hypoxia HB 14 - BRUNO P. CARREIRA, MARIA INÊS MORTE, ANTÓNIO F. AMBRÓSIO, CAETANA M. CARVALHO, INÊS M. ARAÚJO, Nitric oxide from microglia origin impairs neural stem cell proliferation via nitration of the epidermal growth factor receptor HB 15 - SANDRA LOUZADA, FILOMENA ADEGA, RAQUEL CHAVES, Defining the sister rat mammary tumor cell lines HH-16 cl.2/1 and HH-16.cl.4 as an in vitro cell model for Erbb2 HB 16 - NUNO R. DOS SANTOS, Microenvironmental factors participating in T-cell leukemia development HB 17 - MATTHIAS FUTSCHIK, UniHI – Network biology goes to the bench HB 18 - GEISA A.L. GONCALVES, KRISTALA L.J. PRATHER, GABRIEL A. MONTEIRO, DUARTE MIGUEL F. PRAZERES, De novo creation of MG1655derived E. coli strains specifically designed for plasmid DNA production ORAL COMMUNICATIONS: M ARINE BIOTECHNOLOGY MB 1 - KARINE BOUILLY, SANDRA LOUZADA, RAQUEL CHAVES, HENRIQUE GUEDES-PINTO, BAC clones physical mapping in Pacific oyster (Crassostrea gigas) MB 2 - CARLA C.C.R. DE CARVALHO, MARCO P.C. MARQUES, PEDRO FERNANDES, Taking advantage of bacterial adaptation to the marine environment MB 3 - SOLANGE I. MUSSATTO, SILVIA MARTINS, JOSÉ A. TEIXEIRA, Antioxidant phenolic compounds in four edible seaweeds ORAL COMMUNICATIONS: NANOBIOTECHNOLOGY NB 1 - CLÁUDIA R. VISTAS, GUILHERME N.M. FERREIRA, HIV-1 virion infectivity factor detection in a microfluidic platform using an integrated hydrogenated amorphous silicon photodetector NB 2 - S.A.M. MARTINS, J.R.C. TRABUCO, J.P. CONDE, V. CHU, D.M.F. PRAZERES, Integrated photodetection of intracellular calcium in response to the activation of GPCRs NB 3 - BRIGITTE TOMÉ, ROGÉRIO M.M. RODRIGUES, JORGE DE-CARVALHO, SILVIA F. HENRIQUES, NUNO P. MIRA, ISABEL SÁ-CORREIA, GUILHERME N.M. FERREIRA, DNA Transcription factor binding monitored by acoustic wave biosensors: A new transmission line model to extract physical properties from biological films NB 4 - M.A. CERQUEIRA, A.A. VICENTE, J.A. TEIXEIRA, Bio-based nanofilms/coatings for food applications NB 5 - A.S. MACEDO, S.B. SOUTO, E.B. SOUTO, Quality by design (QbD) for SLN produced by solvent displacement: a 22 factorial design level approach NB 6 - C.GONÇALVES, S.A. FERREIRA, P. PEREIRA, S. PEDROSA, F.M. GAMA, Self-Assembled Nanogels for Biomedical Applications NB 7 - P. ALBUQUERQUE, J. GOMES, J. BORDADO, M. REIS, Exposure assessment by determining alveolar deposited surface area of nanoparticles ORAL COMMUNICATIONS: PLANT, ANIMAL AND FOOD BIOTECHNOLOGY PAFB 1 - JOSÉ M. OLIVEIRA, Fermented Beverages – Technological and Analytical Approaches PAFB 2 - RICARDO N. PEREIRA, JOSÉ A. TEIXEIRA, ANTÓNIO A. VICENTE, Effects of Moderate Electric Fields (MEF) on Denaturation of Whey Proteins Solutions PAFB 3 - J. MELO, P.W. ANDREW, M.L. FALEIRO, Revealing the different abilities of Listeria monocytogenes in assembly of adaptation response in a cheese-simulated medium PAFB 4 - CARLA CARVALHO, SÍLVIO SANTOS, HUGO OLIVEIRA, LEON KLUSKENS, JOANA AZEREDO, Exploiting the biotechnological potential of bacteriophages towards food safety PAFB 5 - A. VENÂNCIO, C. SOARES, T. CALADO, A.S. VILA-CHÃ, H. MORALES, L. ABRUNHOSA, C. SANTOS, R. PATERSON, N. NELSON, The effect of food processing on the fate of mycotoxins PAFB 6 - F. COSME, L. FILIPE-RIBEIRO, A. MENDES-FAIA, Potential alternatives to potassium caseinate for white wine fining: impact on physicochemical and sensory characteristics PAFB 7 - L. PEREIRA, J.R.A. FERNANDES, P.A.S. JORGE, H. GUEDES-PINTO , P. MARTINS-LOPES, Wine traceability: molecular and biosensor approach PAFB 8 - D. TOUBARRO, N. BALASUBRAMANIAN, M. TEIXEIRA, R. FERREIRA, V. GOUVEIA, M. RAPOSO, C. CABRAL, N. SIMÕES Sampling and exploitation of Azorean micro-biodiversity PAFB 9 - M.S. LOPES, D. MENDONÇA, A. DA CÂMARA MACHADO Molecular tools for the characterisation of the Lusitano horse PAFB 10 - MÁRIO R. SANTOS, ANDREIA T. MARQUES, JÖRG D. BECKER, LEONILDE M. MOREIRA, Involvement of the TetR transcriptional regulator SMc03169 in pH sensitivity and contribution to nodulation competitiveness in Sinorhizobium meliloti PAFB 11 - NELSON SIMÕES, DUARTE TOUBARRO, NATESAN BALASUBRAMANIAN, GISELA NASCIMENTO, MÓNICA MARTINEZ, Insecticidal proteins released by an entomopathogenic nematode PAFB 12 - JORGE M.S. FARIA, A. CRISTINA FIGUEIREDO, Effect of Bursaphelenchus xylophilus inoculation on Pinus pinaster volatiles: juvenile trees versus in vitro cultures PAFB 13 - D. MENDONÇA, M.S. LOPES, L. MONTEIRO, S. LUNA, C. SILVA, M. SIMAS, S. BETTENCOURT, A. Biotechnological tools on the conservation and valorisation of Azorean plant resources DA CÂMARA MACHADO, PAFB 14 - MARTA D. MENDES, A. CRISTINA FIGUEIREDO, HELENA TRINDADE, Expression of a putative monoterpene synthase along Thymus caespititius micropropagation PAFB 15 - FERNANDA LEAL, SANDRA MARTINS, ANA RAQUEL SANTOS, TATIANA SORIANO, CARINA ALVES, ANA CLÁUDIA COELHO, ISABEL GAIVÃO, Essays of genotoxicity, antigenotoxicity and antifungic activity in aromatic and medicinal in vitro plants PAFB 16 - P. MONJARDINO, S. ROCHA, A.C.TAVARES, R. FERNANDES, P. SAMPAIO, R. SALEMA, A. DA CÂMARA MACHADO, A new model for ingrowth formation on maize endosperm transfer cells PAFB 17 - M. SALGADO, M. HORTA, C. BURDINE, A. CRAVADOR, Developing an approach to produce cork oak resistant/tolerant to Phytophthora PAFB 18 - BRUNO MEDRONHO, MARIA GRAÇA MIGUEL, BJÖRN LINDMAN, ANABELA ROMANO, Rationalizing Cellulose (In)solubility: Basic Physicochemical Aspects, Strategies and Controversial Thoughts ORAL COMMUNICATIONS: SYSTEMS AND SYNTHETIC BIOLOGY SSB 1 - L.R. RODRIGUES, J.L. RODRIGUES, C.D. MACHADO, L. KLUSKENS, M. MOTA, I. ROCHA, E.C. FERREIRA, SYNBIOBACTHER – Engineering “therapeutic” bacteria SSB 2 - SÓNIA CARNEIRO, RAFAEL CARREIRA, EUGÉNIO C. FERREIRA, ISABEL ROCHA, Investigating metabolic responses in recombinant E. coli cultures using metabolomics and fluxomics data SSB 3 - ANÁLIA LOURENÇO, SÓNIA CARNEIRO, ISABEL ROCHA, EUGÉNIO C. FERREIRA, Exploring current knowledge on Escherichia coli stringent response via text mining SSB 4 - MIGUEL C. TEIXEIRA, NUNO P. MIRA, PAULO J. DIAS, CATARINA COSTA, CARLA PIRES, ARTUR B. LOURENÇO, TÂNIA R. CABRITO, SANDRA C. DOS SANTOS, ISABEL SÁ-CORREIA, Gene and genomic regulatory networks controlling chemical stress resistance in yeasts SSB 5 - NUNO P. MIRA, SÍLVIA F. HENRIQUES, MIGUEL C. TEIXEIRA, MARGARIDA PALMA, SANDRA C. DOS SANTOS, ISABEL SÁ-CORREIA, Haa1pdependent regulatory network of the yeast response to acetic acid stress SSB 6 - ÓSCAR DIAS, DANIEL GOMES, EUGÉNIO C. FERREIRA, LUCÍLIA DOMINGUES, ISABEL ROCHA, Reconstructing genome-scale metabolic models with merlin: the Ashbya gossypii case study SSB 7A - ANDREIA MADEIRA, CLÁUDIA L. DA SILVA, FRANCISCO DOS SANTOS, JOAQUIM M. S. CABRAL, ISABEL SÁ-CORREIA, Human Mesenchymal Stem Cells expression program upon extended ex-vivo cultivation, as revealed by 2-DE- based quantitative proteomics SSB 7B - TÂNIA R. CABRITO, MIGUEL C. TEIXEIRA, ESTELLE REMY, PAULA DUQUE, ISABEL SÁ-CORREIA, Design and construction of plants resistant to agricultural relevant stresses through understanding the role of putative efflux pumps in yeast and plant models OTHER COMMUNICATIONS ANABELA COELHO, MARIA AMÉLIA LEMOS, FRANCISCO LEMOS, FERNANDO RAMÔA RIBEIRO, GONÇALO CAEIRO, Middle Distillate Production by Oligomerization of 1- Hexene over ZSM-5 Zeolite ANABELA COELHO, PALOMA CAÑADAS, MAJA JAŚKIEWICZ, FRANCISCO LEMOS, MARIA AMÉLIA LEMOS, Catalytic Degradation of Polyethylene A.M.T. MATA, N.D. LOURENÇO, H.M. PINHEIRO, Can starvation stress alter the inhibitory effect of respiratory poisons on dye bioreduction? MANUELA ADRIANO MOREIRA, NÍDIA DANA LOURENÇO, HELENA MARIA PINHEIRO, Characterization of the microbial population dynamics in sequencing batch reactors used for azo dye bioconversion D.P.C. DE BARROS, S.R.R. CAMPOS, P.P. MADEIRA, A. AZEVEDO, A.M. BAPTISTA, M.R. AIRES-BARROS, Partitioning behavior of proteins in aqueous two-phase systems P.C. PEREIRA, M.J. CEBOLA, M.G. BERNARDO-GIL, Supercritical fluid extraction of Myrtus communis L.: Optimization of the Extraction Parameters Using the central Composite Non-Factorial Design ANA S. FERREIRA, INÊS N. SILVA, JÖRG D. BECKER, SIOBBÁN MCCLEAN, MAIRE CALLAGHAN, RUTH PILKINGTON, MICHAEL GIVSKOV, ROBERT P. RYAN, FÁBIO FERNANDES, LEONILDE M. MOREIRA, Involvement of the BceF tyrosine kinase in stress response, biofilm formation and virulence in Burkholderia cepacia IST408 clinical isolate P.H. OLIVEIRA, J.S. BOURA, M.M. ABECASIS, J. GIMBLE, C. LOBATO DA SILVA, J.M.S. CABRAL, Effect of hypoxia and long-term culture on the genomic stability and mitochondrial performance of human mesenchymal stem cells expanded in vitro for clinical applications PEDRO Z. ANDRADE, FRANCISCO DOS SANTOS, GRAÇA ALMEIDA-PORADA, CLÁUDIA LOBATO DA SILVA, JOAQUIM .M.S. CABRAL, Bioengineering approaches for the optimization of the ex-vivo expansion of hematopoietic stem/progenitor cell for cell therapy FRANCISCO DOS SANTOS, CLÁUDIA LOBATO DA SILVA, PEDRO Z. ANDRADE, MANUEL M. ABECASIS, JEFFREY GIMBLE, ANDREW CAMPBELL, SHAYNE BOUCHER, ERIC ROOS, SANDRA KULIGOWSKI, LUCAS CHASE, MOHAN VEMURI, JOAQUIM M.S. CABRAL, Scalable Expansion of Human Mesenchymal Stem Cells using a Microcarrier-based System under Serum-free and Xeno-free Conditions CARLOS A.V. RODRIGUES, MARIA MARGARIDA DIOGO, CLÁUDIA LOBATO DA SILVA, JOAQUIM M.S. CABRAL, Design And Operation of A Bioreactor System For The Expansion of Mouse Embryonic Stem Cell-Derived Neural Stem Cells on Microcarriers V. VIEIRA, J.F. FANGUEIRO, A.M. SILVA, E.B. SOUTO, Characterization of the physicochemical performance of nimesulide-loaded lipid nanoparticulates I.S. SANTOS, A. ROSMANINHO, E.B. SOUTO, Lipid profile solubility of a model NSAID for topical drug delivery M. HORTA, A.C. COELHO, A. CRAVADOR, Preliminary GO annotation of the transcriptomes of Quercus suber roots infected and non-infected with Phytophthora cinnamomi zoospores M. HORTA, A. PÈREZ-SIERRA, A. SOLLA , A. CRAVADOR, T. JUNG, Biological pollution: Phytophthora species threatening forest nurseries and natural ecosystems in Portugal S. ROCHA, A.C. TAVARES, R. FERNANDES, P. SAMPAIO, R. SALEMA, A. DA CÂMARA MACHADO, P. MONJARDINO, Flange and reticulate wall ingrowth formation in developing maize endosperm transfer cells C.D. MELO, D. MENDONÇA, A. DA CÂMARA MACHADO, Arbuscular mycorrhizal fungi (AMF) associated to endemic wood plants of the Native forest of the Azores archipelago MÁRIO TEIXEIRA, RICARDO FERREIRA, CARLA CABRAL, LUÍSA OLIVEIRA, NELSON SIMÕES, Biodiversity of biological control agents collected in Azores S. LUNA, A.C. TAVARES, M.G. SILVEIRA, A. DA CÂMARA MACHADO, Effect of extracts of Vaccinium cylindraceum on protein profile of staphylococcus aureus A.C. TAVARES, S. LUNA, M.G. DA SILVEIRA, A. DA CÂMARA MACHADO, Flow cytometric assessment of the antimicrobial activity of Vaccinium cylindraceum extracts against Staphylococcus aureus A. CONDE, J.M. NOVAIS, A field experiment in an extreme environment of the Tagus estuary: an intertidal habitat in oligohaline transitional waters