XII Congresso Brasileiro de Ecotoxicologia
25 a 28 de setembro de 2012
Porto de Galinhas – PE
ALTERATED GENE EXPRESSION PROFILE OF PEROXIREDOXINS V AND VI IN
BRONCHIAL EPITHELIAL CELLS EXPOSED TO DIESEL EXHAUST PARTICLES
Robson Seriani1; Lucas G. Morais2; Denis M. S. Abessa2; Marcos A. Oliveira2; Elnara M. Negri1;
Paulo H. N. Saldiva1; Thais Mauad1; Mariângela Macchione1
[email protected] (Faculdade de Medicina – USP, SP,SP)
[email protected]. (Universidade Estadual Paulista, Campus do Litoral Paulista, SP)
2
[email protected] (Universidade Estadual Paulista, Campus do Litoral Paulista, SP)
2
[email protected] (Universidade Estadual Paulista, Campus do Litoral Paulista, SP)
1
[email protected] (Faculdade de Medicina – USP, SP,SP)
1
[email protected] (Faculdade de Medicina – USP, SP,SP)
1
[email protected] (Faculdade de Medicina – USP, SP,SP)
1
[email protected] (Faculdade de Medicina – USP, SP,SP).
1
2
Diesel exhaust is a major contributor to various types of air pollution, including smog-forming oxides of
nitrogen (NOx) and fine particles. Additionally, chemical components of some particles, particularly
combustion products, have been shown to cause aggravation of preexisting pulmonary diseases and some
kinds of cancer. Several lines of evidences have revealed that oxidative stress plays a central role in the
bronchopulmonary pathogenesis and a novel family of anti-oxidative proteins, designated as peroxiredoxin
(Prx), has been correlated to several human diseases but studies aiming the relationships to the
bronchopulmonary illness with this proteins are very scarce or inexistent. In humans six Prx isoforms were
described to date named (PrxI-PrxVI). Among them, PrxV and PrxVI may represent an important
antioxidant defense in bronchopulmonary cells, since both enzymes are able to reduce hydrogen peroxide,
alkyl hydroperoxides, peroxynitrite in cytosol (PrxVI), mitochondria and peroxisomes (PrxV), where the last
ones are major intracellular sources of reactive oxygen and nitrogen species. PrxVI is believed to serve as an
important protective function against oxidative stress in lung tissue, which is always exposed to high
concentration of inhalable particles that produce oxidative stress and inflammation. Thus, this study
evaluated gene expression of PrxV and PrxVI in human bronchial epithelial cells (BEAS-2B) exposed of
diesel exhaust particles (DEP-100 µg/ml) at 15, 30 and 60 minutes and control group. Total RNA was
isolated using Trizol reagent and the cDNA was synthesized by reverse transcription using oligo-dT. PrxV
and PrxVI cDNAs were analyzed quantitatively by RT-PCR in triplicate and compared against a control
group. PrxV was low expression in control group, and showing an increased of expression after exposute to
DEP at 15 minutes, followed by a decrease after 30 minutes and overexpression after 60 minutes. PrxVI had
low expression in control group, and to DEP showed an overexpression after 60 minutes. These results
indicate that gene expression of peroxirredoxins varies in short-time in response to pollution insults and may
be linked with its function and specificity, however in 60 minutes both proteins overexpressed, that could be
associated with time activation of both gene expression in these cells.
Keywords: peroxiredoxins, bronchial epithelial cells, air pollution
Sociedade Brasileira de Ecotoxicologia (SBE)
Universidade Federal de Pernambuco (UFPE)
632