CV D.M.F. Prazeres
Curriculum vitae Duarte Miguel de França Teixeira dos Prazeres
1. General
Position: Associate Professor
Affiliation: Bioengineering Department, Instituto Superior Técnico (IST), Universidade de Lisboa
Work address: Instituto Superior Técnico, Av. Rovisco Pais, 1049-001 Lisbon, Portugal
Date and place of birth: June 17, 1966, Madeira, Portugal
E-mail: [email protected] , Phone: +351-218419133
2. Education
1. Diploma in Chemical Engineering, University of Coimbra, Portugal, 1989.
2. Ph.D. Chemical Engineering, Instituto Superior Técnico, Technical University of Lisbon,
Lisbon, Portugal, 1993.
3. Post Doctoral fellow at the Chemical Engineering Department of the Massachusetts Institute
of Technology, Cambridge, Oct. 1996- July 1997, USA.
4. “Agregação” in Chemical Engineering, Instituto Superior Técnico, Technical University of
Lisbon, Lisbon, Portugal, 2003.
5. Visiting Scholar at the Engineering Systems Division do Massachusetts Institute of
Technology, Cambridge, Mar. 2007-May 2007.
3. Teaching (1992-2014)
Subjects: Heat, Mass and Momentum Transfer, Separation and Purification of Biomolecules,
Enzymatic Engineering, Chemical Engineering Laboratories, Nucleic Acids Engineering,
Bioengineering and Market, Bioentrepreneurship, Biomimicry, Bioethics, Gene Therapy,
Dissertation in Biological Engineering.
Target audience: Graduation in Chemical Engineering and Biological Engineering; Masters in
Biological Engineering, Biomedical Engineering, Biotechnology and Bioengineering and
Nanosystems; Doctoral programs in Bioengineering.
Coordination: Integrated Master in Biological Engineering (2012-present)
4. Current research activities
1. Plasmid biopharmaceuticals: Development of products and processes; 2. DNA, protein and
cell microchips; 3. Miniaturization of GPCR screening assays. 4. Molecular Bioengineering and
Biomimetics.
5. Publications
5.1 BOOKS
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Prazeres, D.M.F., "Plasmid Biopharmaceuticals: Basics, Applications and Manufacturing",
2011, John Wiley & Sons, Inc., New York. ISBN: 978-0-470-23292-7
5.2 CHAPTERS IN BOOKS (17)
16 Monteiro, G.A., Silva, M.S., Henriques, A.M., Carvalho, J., Atouguia, J., Fevereiro, M.,
Prazeres, D.M.F., “Advances in DNA Vaccination: Design, Immunology and Manufacturing”
in Veterinary Immunology and Immunopathology, L. Nermann and S. Meier (eds.),
Nova Science Publishers Inc., New York, 2010, pp 129-155.
17
Oliveira, P.H., Prazeres, D.M.F., Monteiro, G.A., “Structural and Segregational Instability in
Plasmid Biology”, in Plasmids – Genetics, Applications and Health, F. E. R. Gonzales
and M. I. Lopez (eds.), Nova Science Publishers Inc., New York, 2012, pp 79-100.
5.3 SCIENTIFIC PUBLICATIONS (167)
147. Jóskowiak, A., Santos, M., Prazeres, D.M.F., Chu, V., Conde, J.P. “Integration of Thin Film
Amorphous Silicon Photodetectors with Lab-on-a-chip for Monitoring Protein Fluorescence
in Solution and in Live Microbial Cells”, Sensors Act. B: Chemical 156 (2011) 662-667.
148. Gomes, G.A., Azevedo, A.M., Aires-Barros, M.R., Prazeres, D.M.F., “Studies on the
Adsorption of Cell Impurities from Plasmid-Containing Lysates to Phenyl-Boronic Acid
Chromatographic Beads”, J. Chromatogr. A, 1218 (2011) 8629-8637.
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CV D.M.F. Prazeres
149. Novo, P., Prazeres, D.M.F., Chu, V., Conde, J.P., “Microspot-based ELISA in Microfluidics:
Chemiluminescence and Colorimetry Detection Using Integrated Thin-film Hydrogenated
Amorphous Silicon Photodiodes”, Lab-on-a-Chip 11 (2011) 4063-4071.
150. Martins, D.C., Prazeres, D.M.F., Chu, V., Conde, J.P., “Label-free Detection of DNA
Immobilization and Hybridization by Streaming Current Measurements in Microchannels”,
App. Physics Lett. 99 (2011) 183702.
151. Gonçalves, G.A.L., Bower, D.M., Prazeres, D.M.F., Monteiro, G.A., Prather, K.L.J.,
“Rational Engineering of Escherichia coli Strains for Plasmid Biopharmaceutical
Manufacturing”, J. Biotechnol., 7 (2012) 251-261.
152. Lewis, L.A., Astatke, M., Umekubo, P.T., Alvi, S., Saby, R., Afrose, J., Oliveira, P.H.,
Monteiro, G.A., Prazeres, D.M.F., “Protein-DNA Interactions Define the Mechanistic
Aspects of Circle Formation and Insertion Reactions in IS2 Transposition”, Mobile DNA, 3
(2012) 1-24.
153. Toledo, M.A.S., Janissen, R., Favaro, M.T.P., Cotta, M.A., Monteiro, G.A., Prazeres,
D.M.F., Souza, A.P., Azzoni, A.R.,“Development of a Recombinant Fusion Protein Based
on the Dynein Light Chain LC8 for Non-viral Gene Delivery”, J. Controlled Release, 159
(2012) 222-231.
154. Jóskowiak, A., Stasio, N., Chu, V., Prazeres, D.M.F., Conde, J.P. “Integrated Detection of
Intrinsic Fluorophores in Live Microbial Cells Using an Array of Thin Film Amorphous
Silicon Photodetectors”, Biosensors & Bioelectronics, 36 (2012) 242-249.
155. Gomes, G.A., Azevedo, A.M., Aires-Barros, M.R., Prazeres, D.M.F., “Validation and Scaleup of Plasmid DNA Purification by Phenyl-Boronic Acid Chromatography”, J. Sep. Sci., 35
(2012) 3190-3196.
156. Martins, S.A.M., Trabuco, J.R.C., Monteiro, G.A., Chu, V., Conde, J.P., Prazeres, D.M.F.
“Towards the Miniaturization of GPCR-based Live-cell Screening Assays”, Trends
Biotechnol., 30 (2012) 566-574.
157. Gonçalves, G.A.L., Prazeres, D.M.F., Monteiro, G.A., Prather, K.L.J., “De Novo Creation of
MG1655-derived Escherichia coli Strains Specifically Designed for Plasmid DNA
Production”, App. Microbiol. Biotechnol., 97 (2013) 611-620.
158. Novo, P., Moulas, G., Prazeres, D.M.F., Chu, V., Conde, J.P., “Detection of Ochratoxin A in
Wine and Beer by Chemiluminescence-based ELISA in a Microfluidic Structure with
Integrated Optical Detection”, Sensors Act. B: Chemical 176 (2013) 232-240.
159. Donato, S.S., Chu, V., Prazeres, D.M.F., Conde, J.P., “Metabolic Viability of E. coli Trapped
by Dielectrophoresis in Microfluidics”, Electrophoresis 34 (2013) 575-582.
160. Martins, D.C., Chu, V, Prazeres, D.M.F., Conde, J.P., “Streaming Current Measurements in
a Single PDMS/glass Microchannel with Integrated Polarizable Electrodes”, Microfluidics
and Nanofluidics 15 (2013) 361-376.
161. De la Vega, J., Braak, B., Monteiro, G.A., Azzoni, A.R., Prazeres, D.M.F., “Impact of
Plasmid Quality on Lipoplex-Mediated Transfection”, J. Pharm. Sci. 102 (2013) 3932-3941.
162. Raiado, L.P., Prazeres, D.M.F., Mateus, M., “Impact of Plasmid Size on the Purification of
Model Plasmid DNA Vaccines by Phenyl Membrane Adsorbers”, J. Chromatog. A, 1315
(2013) 145-151.
163. Martins, S.A.M., Moulas, G., Trabuco, J.R.C., Monteiro, G.A., Chu, V., Conde, J.P.,
Prazeres, D.M.F., “Monitoring Intracellular Calcium in Response to GPCR Activation Using
Thin-film Silicon Photodiodes with Integrated Fluorescence Filters”, Biosensors
Bioelectronics, 52 (2014) 232-238.
164. Simcikova, M., Prather, K.L.J., Prazeres, D.M.F., Monteiro, G.A., “On the Dual Effect of
Glucose During Production of pBAD/AraC-based Minicircles”, Vaccine, 32 (2014) 28432846.
165. Gonçalves, G.A.L., Prather, K.L.J., Monteiro, G.A., Prazeres, D.M.F., “Engineering of
Escherichia coli Strains for Plasmid Biopharmaceutical Production: Scale-up Challenges”,
Vaccine, 32 (2014) 2847-2850.
166. Raiado, L.P., De la Vega, J., Prazeres, D.M.F., Mateus, M., “Development of a Phenyl
Membrane Chromatography–based Process Yielding Pharmaceutical Grade Plasmid DNA
for Mammalian Cells Transfection”, J. Chromatogr. A, 1337 (2014) 67-74.
167. Rosa, A.M.M., Louro, A.F., Martins, S.A.M., Silva, J.J.I., Azevedo, A.M., Prazeres, D.M.F.
“Capture and Detection of DNA Hybrids on Paper via the Anchoring of Antibodies with
Fusions of Carbohydrate Binding Modules with ZZ-domains”, Anal. Chem., 86 (2014) 43404347.
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