João António de Sampaio Rodrigues Queiroz https://www.ubi.pt/SSL/Pagina_Pessoal.aspx?id=jqueiroz http://orcid.org/0000-0002-3096-8325 (Junho 2014) Patentes D.M.F. Prazeres, M.M. Diogo, J.A. Queiroz, “Process for production and purification of plasmid DNA”, Patent Number PT 102491 R; WO 02/04027 A1; United States Patent 7,169,917, January 30, 2007. C. Cruz, E. Cairrao, S. Silvestre, L. Breitenfeld, P. Almeida, J.A. Queiroz, “Método para a marcação da mitocôndria e do retículo endoplasmático utilizando compostos macrocíclicos fluorescentes”, Patent Number PT 105419, 2012. Capítulos de Livros J.M.L. Rodilla, M.I. Ismael, J.A. Queiroz, J.A. Figueiredo, R. Martins, “Transformation of a Labdane by an Enzymatic Treatment”, Natural Products in the New Millennium: Prospects and Industrial Application, A.P. Rauter, F.B. Palma, J. Justino, M.E. Araújo, S.P. Santos (eds.), Kluwer Academic Publishers, The Netherlands: Dordrecht, pp. 437440, 2002. M.M. Diogo, J.A. Queiroz, D.M.F. Prazeres, “Purification of Plasmid DNA Vectors Produced in Escherichia coli for Gene Therapy and DNA Vaccine Applications”, Microbial Processes and Products. Methods in Biotechnology, vol.18, J.-L. Barredo (ed.), Humana Press, Totowa, NJ, pp. 165-178, 2005. F. Sousa, L. Passarinha, J.A. Queiroz, "Biomedical application of plasmid DNA in gene therapy: A new challenge for chromatography", Biotechnology and Genetic Engineering Reviews, 26, 83-116, 2009. A.P. Gomes, J.F. Mano, J.A. Queiroz, I.C. Gouveia, "Assessment of bacteria-textile interactions using Scanning Electron Microscopy: A study on LbL chitosan/alginate coated cotton”, Microscopy: Science, Technology, Applications and Education, vol.1, A. Méndez-Vilas, J. Díaz Álvarez (eds.), Formatex Research Center, pp. 286-292, 2010. L. Paulo, M. Oleastro, E. Gallardo, J.A. Queiroz, F. Domingues, “Antimicrobial properties of resveratrol: a review”, Science against Microbial Pathogens: Communicating Current Research and Technological Advances, Microbiology Book Series, A. Méndez-Vilas (ed.), Formatex, pp. 1225-1235, 2011. CT Tomaz, J.A. Queiroz, “Hydrophobic Interaction Chromatography”, Liquid Chromatography: Fundamentals and Instrumentation, S. Fanali, P.R. Haddad, C.F. Poole, P. Schoenmakers, D. Lloyds (eds.), Elsevier, pp 121-141, 2013. Publicações científicas/Artigos mais relevantes 1. J.A. Queiroz, F.A.P. Garcia, J.M.S. Cabral, “Hydrophobic Interaction Chromatography of Chromobacterium viscosum Lipase”, Journal of Chromatography A, 707, 137-142, 1995. 2. J.A. Queiroz, F.A.P. Garcia, J.M.S. Cabral, “Partitioning of Chromobacterium viscosum Lipases in Aqueous Two-Phase Systems”, Bioseparation, 5, 307-311, 1995. 3. J.A. Queiroz, F.A.P. Garcia, J.M.S. Cabral, “Hydrophobic Interaction Chromatography of Chromobacterium viscosum Lipase on Polyethylene Glycol Immobilized on Sepharose”, Journal of Chromatography A, 734, 213-219, 1996. 4. M.M. Diogo, J.M.S. Cabral, J.A. Queiroz, “Preliminary Study on Hydrophobic Interaction Chromatography of Chromobacterium viscosum Lipase on Polypropylene Glycol Immobilized on Sepharose”, Journal of Chromatography A, 796, 177-180, 1998. 5. J.A. Queiroz, F.A.P. Garcia, J.M.S. Cabral, “Purification of a Chromobacterium viscosum Lipase by Hydrophobic Interaction Chromatography”, Isolation & Purification, 3, 1-7, 1999. 6. M.M. Diogo, S. Silva, J.M.S. Cabral, J.A. Queiroz, “Hydrophobic Interaction Chromatography of Chromobacterium viscosum Lipase on Polypropylene Glycol Immobilized on Sepharose”, Journal of Chromatography A, 849, 413-419, 1999. 7. J.F. Cascalheira, J.A. Queiroz, “Kinetic Study of the Cellobiase Activity of Trichoderma reesei Cellulase Complex at High Substrate Concentrations”, Biotechnology Letters, 21, 651-655, 1999. 8. M.M. Diogo, J.A. Queiroz, G.A. Monteiro, D.M.F. Prazeres, “Separation and Analysis of Plasmid Denatured Forms using Hydrophobic Interaction Chromatography”, Analytical Biochemistry, 275, 122-124, 1999. 9. M.A. Esquibel-King, A.C. Dias-Cabral, J.A. Queiroz, N.G. Pinto, “A Study of Hydrophobic Interaction Adsorption of Bovine Serum Albumin under Overload Conditions using Flow Microcalorimetry”, Journal of Chromatography A, 865, 111122, 1999. 10. C.T. Tomaz, J.A. Queiroz, "Studies on the Chromatographic Fractionation of Trichoderma reesei Cellulases by a Hydrophobic Interaction" Journal of Chromatography A, 865, 123-128, 1999. 11. F.C. Domingues, J.A. Queiroz, J.M.S. Cabral, L.P. Fonseca, “The Influence of Culture Conditions on Mycelial Structure and Cellulase Production by Trichoderma reesei Rut C-30”, Enzyme and Microbial Technology, 26, 394-401, 2000. 12. M.M. Diogo, J.A. Queiroz, G.A. Monteiro, S.A.M. Martins, G.N.M. Ferreira, D.M.F. Prazeres, “Purification of a Cystic Fibrosis Plasmid Vector for Gene Therapy using Hydrophobic Interaction Chromatography”, Biotechnology and Bioengineering, 68, 576-583, 2000. 13. M.M. Diogo, S. Ribeiro, J.A. Queiroz, G.A. Monteiro, P. Perrin, N. Tordo, D.M.F. Prazeres, “Scale-up of Hydrophobic Interaction Chromatography for the Purification of a DNA Vaccine Against Rabies”, Biotechnology Letters, 22, 1397-1400, 2000. 14. J.A. Queiroz, C.T. Tomaz, J.M.S. Cabral, “Hydrophobic Interaction Chromatography of Proteins”, Journal of Biotechnology, 87, 143-159, 2001. 15. F.C. Domingues, J.A. Queiroz, J.M.S. Cabral, L.P. Fonseca, “Production of Cellulases in Batch Culture using a Mutant Strain of Trichoderma reesei Growing on Soluble Carbon Source”, Biotechnology Letters, 23, 771-775, 2001. 16. M.M. Diogo, S. Ribeiro, J.A. Queiroz, G.A. Monteiro, N. Tordo, P. Perrin, D.M.F. Prazeres, “Production, Purification and Analysis of an Experimental DNA Vaccine Against Rabies”, The Journal of Gene Medicine, 3, 577-584, 2001. 17. C.T. Tomaz, D. Duarte, J.A. Queiroz, “Comparative Study on the Fractionation of Cellulases on Some Hydrophobic Interaction Chromatography Adsorbents”, Journal of Chromatography A, 944, 211-216, 2002. 18. M.M. Diogo, J.A. Queiroz, D.M.F. Prazeres, “Hydrophobic Interaction Chromatography of Homo-oligonucleotides on Derivatized Sepharose CL-6B. Application of the Solvophobic Theory”, Journal of Chromatography A, 944, 119-128, 2002. 19. A.C. Dias-Cabral, N.G. Pinto, J.A. Queiroz, “Studies on Hydrophobic Interaction Adsorption of Bovine Serum Albumin on Polypropylene Glycol-Sepharose under Overloaded Conditions”, Separation Science and Technology, 37, 1505-1520, 2002. 20. C.T. Tomaz, A.S. Rocha, J.A. Queiroz, “Hydrophobic Interaction Chromatography of Trichoderma reesei Cellulases on Polypropylene Glycol-Sepharose”, Separation Science and Technology, 37, 1641-1651, 2002. 21. M.M. Diogo, J.A. Queiroz, D.M.F. Prazeres, “Studies on the Retention of Plasmid DNA and Escherichia coli Nucleic Acids Chromatography”, Bioseparation, 10, 211-220, 2002. by Hydrophobic Interaction 22. A.C. Pardal, M.J. Nunes, A.M. Gama, J.A. Queiroz, P. Almeida, “Preliminary Studies on the use of Cyanines as Ligands in Dye-Affinity Chromatography of Proteins”, Coloration Technology, 118, 95-99, 2002. 23. M.M. Diogo, J.A. Queiroz, D.M.F. Prazeres, “Assessment of Purity and Quantification of Plasmid DNA in Process Solutions using High Performance Hydrophobic Interaction Chromatography”, Journal of Chromatography A, 998, 109117, 2003. 24. A.C. Dias-Cabral, J.A. Queiroz, N.G. Pinto, “Effect of Salt and Temperature on the Adsorption of Bovine Serum Albumin on Polypropylene Glycol-Sepharose under Linear and Overloaded Chromatographic Conditions”, Journal of Chromatography A, 1018, 137-153, 2003. 25. M.M. Diogo, D.M.F. Prazeres, N.G. Pinto, J.A. Queiroz, “Hydrophobic Interaction Chromatography of Homo-oligonucleotides on Derivatized Sepharose CL-6B. Using and Relating two Different Models for Describing the Effect of Salt and Temperature on Retention”, Journal of Chromatography A, 1006, 137-148, 2003. 26. C.T. Tomaz, J.A. Queiroz, “Fractionation of Trichoderma reesei Cellulases by Hydrophobic Interaction Chromatography on Phenyl-Sepharose”, Biotechnology Letters, 26, 223-227, 2004. 27. M.M. Diogo, J.A. Queiroz, D.M.F. Prazeres, “Chromatography of Plasmid DNA”, Journal of Chromatography A, 1069, 3-22, 2005. 28. F. Sousa, C.T. Tomaz, D.M.F. Prazeres, J.A. Queiroz, “Separation of supercoiled and open circular Plasmid DNA Isoforms by Chromatography with a Histidine-Agarose Support”, Analytical Biochemistry, 343, 183-185, 2005. 29. S. Ferreira, F. Sousa, J.A. Queiroz, F.C. Domingues, “Improved Recovery of a Fusion Protein Containing the Antigenic Domain 1 of the Human Cytomegalovirus Glycoprotein B”, Biotechnology Letters, 27, 1241-1245, 2005. 30. A.C. Dias-Cabral, A.S. Ferreira, J. Phillips, J.A. Queiroz, N.G. Pinto, “The Effects of Ligand Chain Length, Salt Concentration and Temperature on the Adsorption of Bovine Serum Albumin onto Polypropylene Glycol-Sepharose”, Biomedical Chromatography, 19, 606-616, 2005. 31. L.A. Passarinha, M.M. Diogo, J.A. Queiroz, G.A. Monteiro, L.P. Fonseca, D.M.F. Prazeres, “Production of ColE1 Type Plasmid by Escherichia coli DH5α Cultured under Non-Selective Conditions”, Journal of Microbiology and Biotechnology, 16, 20-24, 2006. 32. L.A. Passarinha, M.J. Bonifácio, J.A. Queiroz, “The Effect of Temperature on the Analysis of Methanephrine for Catechol-O-Methyltransferase Activity Assay by HPLC with Electrochemical Detection”, Biomedical Chromatography, 20, 937-944, 2006. 33. F. Sousa, S. Ferreira, J.A. Queiroz, F.C. Domingues, “Production of a Fusion Protein Containing the Antigenic Domain 1 of the Human Cytomegalovirus Glycoprotein B”, Journal of Microbiology and Biotechnology, 16, 1026-1031, 2006. 34. F. Sousa, A. Melo, S. Almeida, P. Paixão, J.A. Queiroz, F.C. Domingues, “Isolation of a Fusion Protein Containing the Antigenic Domain 1 of the Human Cytomegalovirus Glycoprotein B and its Application in ELISA Tests”, Biotechnology Letters, 28, 73-77, 2006. 35. F. Sousa, S. Freitas, A. Azzoni, D.M.F. Prazeres, J.A. Queiroz, “Selective Purification Plasmid DNA from Clarified Cell Lysates with a Single Histidine-Agarose Chromatography Step”, Biotechnology and Applied Biochemistry, 45, 131-140, 2006. 36. L.A. Passarinha, M.J. Bonifácio, J.A. Queiroz, “Comparative Study on the Interaction of Recombinant Soluble Catechol-O-Methyltransferase on Some Hydrophobic Adsorbents”, Biomedical Chromatography, 21, 430-438, 2007. 37. A. Oudia, E. Mészáros, R. Simões, J.A. Queiroz, E. Jakab, “ Pyrolysis-GC/MS and TG/MS Study of Mediated Laccase Biodelignification of Eucalyptus globulus Kraft Pulp”, Journal of Analytical and Applied Pyrolysis, 78, 233-242, 2007. 38. F. Sousa, D.M.F. Prazeres, J.A. Queiroz, “Dynamic binding capacity of plasmid DNA in histidine-agarose chromatography”, Biomedical Chromatography, 21, 993-998, 2007. 39. F. Sousa, D.M.F. Prazeres, J.A. Queiroz, “Circular dichroism investigation of the effect of plasmid DNA structure on retention in histidine chromatography", Archives of Biochemistry and Biophysics, 467, 154-162, 2007. 40. M. Barroso, M. Dias, D.N. Vieira, J.A. Queiroz, M. López-Rivadulla, "Development and validation of an analytical method for the simultaneous determination of cocaine and its main metabolite, benzoylecgonine, in human hair by gas chromatography-mass spectrometry", Rapid Communications in Mass Spectrometry, 22, 3320-3326, 2008. 41. R.E.F. Boto, P. Almeida, J.A.Queiroz, “Thiacarbocyanine as ligand in dye-affinity chromatography for protein purification”, Biomedical Chromatography, 22, 278-288, 2008. 42. E. Gallardo, J.A. Queiroz, “The role of alternative specimens on toxicological analysis", Biomedical Chromatography, 22, 795-821, 2008. 43. I. Gouveia, J.M. Fiadeiro, J.A. Queiroz, "Combined bio-carbonization and dyeing of wool: a possibility using cell-wall-degrading enzymes and 1:1 metal-complex dyes", Engineering in Life Sciences, 8, 250-259, 2008. 44. I. Gouveia, J.M. Fiadeiro, J.A. Queiroz, "Enzymatic Application to Remove Plant Residues from Wool: Preliminary Experiments Evaluating the Effect of Trichoderma reesei Cellulases and Aspergillus aculeatus Pectinases and Hemicellulases", AATCC Review, 8, 38-44, 2008. 45. I. Gouveia, J.M. Fiadeiro, J.A. Queiroz, "Enzymatic removal of plant residues from wool: application of experimental design techniques for optimization parameters", Biochemical Engineering Journal, 41, 157-165, 2008. 46. A. Morão, M.T.P. Amorim, A. Lopes, I. Escobar, J.A. Queiroz, "Characterisation of ultrafiltration and nanofiltration membranes from rejections of neutral reference solutes using a model of asymmetric pores", Journal of Membrane Science, 319, 64-75, 2008. 47. A. Oudia, J.A. Queiroz, R. Simões, "Potential and Limitation of Trametes versicolor laccase on Biodegradation of Eucalyptus globulus and Pinus pinaster kraft pulp", Enzyme and Microbial Technology, 43, 144-148, 2008. 48. A. Oudia, J.A. Queiroz, R. Simões, "The influence of operating parameters on the delignification of Eucalyptus globulus kraft pulps in a laccase-violuric acid system", Applied Biochemistry and Biotechnology, 149, 23-32, 2008. 49. L.A. Passarinha, M.J. Bonifácio, P. Soares-da-Silva, J.A. Queiroz, "A new approach on the purification of Recombinant Human Soluble Catechol-O-Methyltransferase from an Escherichia coli extract using Hydrophobic Interaction Chromatography", Journal of Chromatography A, 1177, 287-296, 2008. 50. A. Sousa, L.A. Passarinha, L. Rodrigues, J.A. Teixeira, A. Mendonça, J.A. Queiroz, “Separation of different forms of proteose peptone 3 by hydrophobic interaction chromatography with a dual salt system”, Biomedical Chromatography, 22, 447–449, 2008. 51. F. Sousa, T. Matos, D.M.F. Prazeres, J.A. Queiroz, “Specific recognition of supercoiled plasmid DNA in arginine affinity chromatography”, Analytical Biochemistry, 374, 432-434, 2008. 52. F. Sousa, D.M.F. Prazeres, J.A. Queiroz, "Affinity chromatography approaches to overcome the challenges of purifying plasmid DNA", Trends in Biotechnology, 26, 518-525, 2008. 53. F. Sousa, D.M.F. Prazeres, J.A. Queiroz, "Binding and elution strategy for improved performance of arginine affinity chromatography in supercoiled plasmid DNA purification", Biomedical Chromatography, 23, 160-165, 2009. 54. F. Sousa, D.M.F. Prazeres, J.A. Queiroz, "Improvement of transfection efficiency by using supercoiled plasmid DNA purified with arginine affinity chromatography", Journal of Gene Medicine, 11, 79-88, 2009. 55. R.E.F. Boto, U. Anyanwu, F.Sousa, P. Almeida, J.A. Queiroz, “Thiacarbocyanine as ligand in dye-affinity chromatography for protein purification. II.Dynamic binding capacity using lysozyme as a model”, Biomedical Chromatography, 23, 988-993, 2009. 56. A. Oudia, E. Mészáros, E. Jakab, R. Simões, J.A. Queiroz, A. Ragauskas, L. Novak, “Analytical pyrolysis study of biodelignification of cloned Eucalyptus globulus and Pinus pinaster aiton kraft pulp and residual lignins”, Journal of Analytical and Applied Pyrolysis, 85, 19-29, 2009. 57. A. Sousa, F. Sousa, D.M.F. Prazeres, J.A. Queiroz, “Histidine affinity chromatography of homo-oligonucleotides. Role of multiple interactions on retention”, Biomedical Chromatography, 23, 745-753, 2009. 58. A. Sousa, F. Sousa, J.A. Queiroz, "Selectivity of arginine chromatography in promoting different interactions using synthetic oligonucleotides as model", Journal of Separation Science, 32, 1665-1672, 2009. 59. E. Gallardo, M. Barroso, J.A. Queiroz, “LC/MS: A Powerful Tool in Workplace Drug Testing” Drug Testing and Analysis, 1, 109-115, 2009. 60. A. Morão, J.C. Nunes, F. Sousa, M.T.P. Amorim, I. Escobar, J.A. Queiroz, "Development of a Model for Membrane Filtration of Long and Flexible Macromolecules: Application to Predict Dextran and Linear DNA Rejections in Ultrafiltration", Journal of Membrane Science, 336, 61-70, 2009. 61. F. Silva, L. Passarinha, F. Sousa, J.A. Queiroz, F.C. Domingues, "Influence of growth conditions on plasmid DNA production"; Journal of Microbiology and Biotechnology, 19, 1408-1414, 2009. 62. M. Barroso, E. Gallardo, J.A. Queiroz, “Bioanalytical Methods for the Determination of Cocaine and Metabolites in Human Biological Samples”, Bioanalysis, 1, 977-1000, 2009. 63. E. Gallardo, M. Barroso, J.A. Queiroz, “Current Technologies and Considerations for Drug Bioanalysis in Oral Fluid”, Bioanalysis, 1, 637-667, 2009. 64. A. Palmeira-de-Oliveira, L. Salgueiro, R. Palmeira-de-Oliveira, J. Martinez-deOliveira, C. Pina-Vaz, J.A. Queiroz, A.G. Rodrigues, “Anti-Candida activity of essential oils: A Review”; Mini Reviews in Medicinal Chemistry, 9, 1292-1305, 2009. 65. L. Passarinha, M.J. Bonifácio, J.A. Queiroz,"Application of a fed-batch bioprocess for the heterologous production of hSCOMT in Escherichia coli", Journal of Microbiology and Biotechnology, 19, 972-981, 2009. 66. A. Sousa, F. Sousa, J.A. Queiroz, "Biorecognition of supercoiled plasmid DNA isoform in lysine-affinity chromatography", Journal of Chromatography B, 877, 32573260, 2009. 67. S. Ferreira, A.P. Duarte, M.H. Ribeiro, J.A. Queiroz, F.C. Domingues, "Response surface optimization of enzymatic hydrolysis of Cistus ladanifer and Cystus striatus for bioethanol production", Biochemical Engineering Journal, 45, 192-200, 2009. 68. S. Ferreira, A.P. Duarte, M.H. Ribeiro, J.A. Queiroz, F.C. Domingues, "Influence of buffer systems on Trichoderma reesei Rut C-30 morphology and cellulase production", Electronic Journal of Biotechnology, 12 (3), 2009. 69. F. Sousa, L. Passarinha, J.A. Queiroz, "Biomedical application of plasmid DNA in gene therapy: A new challenge for chromatography", Biotechnology and Genetic Engineering Reviews, 26, 83-116, 2009. 70. A. Silva, R.E.F. Boto, C.T. Tomaz, J.A Queiroz, P. Almeida, "Rhodamine B as ligand for affinity chromatography. Chromatographic studies on derivatized beaded cellulose", Journal of Chromatographic Science, 48, 240-244, 2010. 71. M. Barroso, M. Dias, D.N. Vieira, M. López- Rivadulla, J.A. Queiroz, “Simultaneous quantitation of morphine, 6-acetylmorphine, codeine, 6-acetylcodeine and tramadol in hair using mixed mode solid-phase extraction and gas chromatographymass spectrometry”, Analytical and Bioanalytical Chemistry, 396, 3059-3069, 2010. 72. F. Silva, O. Lourenço, C. Pina-Vaz, A.G. Rodrigues, J.A. Queiroz, F.C. Domingues, “The use of DRAQ5 to monitor intracellular DNA in Escherichia coli by flow cytometry”, Journal of Fluorescence, 20, 907-914, 2010. 73. L. Paulo, S. Ferreira, E. Gallardo, J.A. Queiroz, F.C. Domingues, “Antimicrobial activity and effects of resveratrol on human pathogenic bacteria”, World Journal of Microbiology and Biotechnology, 26, 1533-1538, 2010. 74. V.S. Nunes, M.J. Bonifácio, J.A. Queiroz, L.A. Passarinha, "Assessment of COMT isolation by HIC using a dual salt system and low temperature", Biomedical Chromatography, 24, 858-862, 2010. 75. A. Palmeira-de-Oliveira, M.P. Ribeiro, R. Palmeira-de-Oliveira, C. Gaspar, S. Costa-de-Oliveira, I.J. Correia, J.A. Queiroz, C. Pina-Vaz, J. Martinez-de-Oliveira, A.G. Rodrigues, “Anticandida activity of a chitosan hydrogel: Mechanism of action and cytotoxicity profile”, Gynecologic and Obstetric Investigation, 70, 322-327, 2010. 76. M. Barroso, M. Dias, D.N. Vieira, M. López-Rivadulla, J.A. Queiroz, “Mixed-mode solid-phase extraction for sample cleanup in hair analysis for methadone and its main metabolite”, Biomedical Chromatography, 24, 1240-1246, 2010. 77. S. Ferreira, N. Gil, A.P. Duarte, J.A. Queiroz, F.C. Domingues, “Bioethanol from the portuguese forest residue Pterospartum tridentatum - An evaluation of pretreatment strategy for enzymatic saccharification and sugars fermentation”, Bioresource Technology, 101, 7797-7803, 2010. 78. F. Sousa, C. Cruz, J.A. Queiroz, "Amino acids-nucleotides biomolecular recognition: From biological occurrence to affinity chromatography", Journal of Molecular Recognition, 23, 505-518, 2010. 79. I.C. Gouveia, L.P. Dias, J.M. Fiadeiro, J.A. Queiroz, “Enzymatic Treatments to Improve the Dyeability of Linen Fabrics: Optimization and Empirical Modeling using Experimental Design Techniques”, Textile Research Journal, 80, 1602-1615, 2010. 80. M. Barroso, S. Costa, M. Dias, D.N. Vieira, J.A. Queiroz, M. López-Rivadulla, “Analysis of phenylpiperazine-like stimulantes in human hair as trimethylsilyl derivatives by gas chromatography-mass spectrometry”, Journal of Chromatography A, 1217, 6274-6280, 2010. 81. R. Raposo, M. Barroso, S. Fonseca, S. Costa, J.A. Queiroz, E. Gallardo, M. Dias, “Determination of eight selected organophosphorus insecticides in postmortem blood samples using solid-phase extraction and gas chromatography/mass spectrometry”, Rapid Communications in Mass Spectrometry, 21, 3187-3194, 2010. 82. R. Martins, J.A. Queiroz, F. Sousa, “A new affinity approach to isolate Escherichia coli 6S RNA with histidine-chromatography”, Journal of Molecular Recognition, 23, 519, 524, 2010. 83. A. Sousa, F. Sousa, J.A. Queiroz, “Differential interactions of plasmid DNA, RNA and genomic DNA with amino acid-based affinity matrices”, Journal of Separation Science, 33, 2610-2618, 2010. 84. C. Cruz, E.J. Cabrita, J.A. Queiroz, “Analysis of nucleotides binding to chromatography supports provided by nuclear magnetic resonance spectroscopy”, Journal of Chromatography A, 1218, 3559-3564, 2011. 85. M. Barroso, E. Gallardo, D.N. Vieira, M. López-Rivadulla, J.A. Queiroz. “Hair: a complementary source of bioanalytical information in forensic toxicology”, Bioanalysis, 3, 67-79, 2011. 86. V.M. Gaspar, F. Sousa, J.A. Queiroz, I.J. Correia, “Formulation of chitosan-TPPpDNA nanocapsules for gene therapy applications”, Nanotechnology, 22, 015101, 2011. 87. S. Ferreira, N. Gil, J.A. Queiroz, A.P. Duarte, F.C. Domingues, “An evaluation of the potential of Acacia dealbata as raw material for bioethanol production”, Bioresource Technolology, 102, 4766-4773, 2011. 88. L. Paulo, F.C. Domingues, J.A. Queiroz, E. Gallardo, “Development and Validation of an Analytical Method for the Determination of trans- and cis-Resveratrol in Wine: Analysis of Its Contents in 186 Portuguese Red Wines”, Journal of Agricultural and Food Chemistry, 59, 2157-2168, 2011. 89. L. Paulo, M. Oleastro, E. Gallardo, J.A. Queiroz, F.C. Domingues, “Antihelicobacter pylori and urease inhibitory activities of resveratrol and red wine” Food Research International, 44, 964-969, 2011. 90. F. Silva, O. Lourenço, C. Maia, J.A. Queiroz, F.C. Domingues, “Impact of plasmid induction strategy on overall plasmid DNA yield and E. coli physiology using flow cytometry and real-time PCR”, Process Biochemistry, 46, 174-181, 2011. 91. F. Sousa, J.A. Queiroz, “Supercoiled plasmid quality assessment by analytical arginine-affinity chromatography”, Journal of Chromatography A, 1218, 124-129, 2011. 92. F. Silva, O. Lourenço, J.A. Queiroz, F.C. Domingues, “Bacteriostatic versus bactericidal activity of ciprofloxacin in Escherichia coli assessed by flow cytometry using a novel far-red dye”, Journal of Antibiotics, 64, 321-325, 2011. 93. A. Sousa, D. Bicho, C.T. Tomaz, F. Sousa, J.A. Queiroz, “Performance of a nongrafted monolithic support for purification of supercoiled plasmid DNA”, Journal of Chromatography A, 1218, 1701-1706, 2011. 94. S.R. Costa, M.J. Bonifácio, J.A. Queiroz, L.A. Passarinha, “Analysis of hSCOMT adsorption in bioaffinity chromatography with immobilized amino acids: The influence of pH and ionic strength”, Journal of Chromatography B, 879, 1704-1706, 2011. 95. M. Barroso, E. Gallardo, D.N. Vieira, J.A. Queiroz, M. López-Rivadulla, “Bioanalytical procedures and recent developments in the determination of opiates/opioids in human biological samples”, Analytical and Bioanalytical Chemistry, 400, 1665-1690, 2011. 96. C. Cruz, R.E.F. Boto, P. Almeida, J.A. Queiroz, “Study of specific interactions between nucleotides and dye support by NMR”, Journal of Molecular Recognition, 24, 975-980, 2011. 97. A. Morão, J.C. Nunes, F. Sousa, M.T.P. Amorim, I.C. Escobar, J.A. Queiroz, “Ultrafiltration of supercoiled plasmid DNA: modelling and application”, Journal of Membrane Science, 378, 280-289, 2011. 98. C. Cruz, E.J. Cabrita, J.A. Queiroz, “Screening nucleotide binding to amino acid coated supports by surface plasmon resonance and nuclear magnetic resonance”, Analytical and Bioanalytical Chemistry, 401, 983-993, 2011. 99. F. Silva, S. Ferreira, J.A. Queiroz, F.C. Domingues, “Coriander (Coriandrum sativum L.) essential oil: its antibacterial activity and mode of action evaluated by flow cytometry”, Journal of Medical Microbiology, 60, 1479-1486, 2011. 100. A.Q. Pedro, M.J. Bonifácio, J.A. Queiroz, C.J. Maia, L.A. Passarinha, “A novel prokaryotic expression system for biosynthesis of recombinant human membrane-bound catechol-O-methyltransferase”, Journal of Biotechnology, 156, 141-146, 2011. 101. A. Sousa, F. Sousa, J.A. Queiroz, “Impact of lysine-affinity chromatography on supercoiled plasmid DNA purification”, Journal of Chromatography B, 879, 3507-3515, 2011. 102. A. Sousa, C.T. Tomaz, F. Sousa, J.A. Queiroz, “Successful application of monolithic innovative technology using a carbonyldiimidazole disk to purify supercoiled plasmid DNA suitable for pharmaceutical applications, Journal of Chromatography A, 1218, 8333-8343, 2011. 103. D. Costa, A.J.M. Valente, M. Graça Miguel, J.A. Queiroz, “Gel network photodisruption: a new strategy for the codelivery of plasmid DNA and drugs”, Langmuir, 27, 13780-13789, 2011. 104. C. Cruz, E. Cairrão, S. Silvestre, L. Breitenfeld, P. Almeida, J.A. Queiroz, “Targeting of mitochondria-endoplasmic reticulum by fluorescent macrocyclic compounds”, PLoS One, 6, e27078, 2011. 105. A. Palmeira-de-Oliveira, L.A. Passarinha, C. Gaspar, R. Palmeira-de-Oliveira, B. Sarmento, J. Martinez-de-Oliveira, C. Pina-Vaz, A.G. Rodrigues, J.A. Queiroz, “The relationship between Candida species charge density and chitosan activity evaluated by ion-exchange chromatography”, Journal of Chromatography B, 879, 3749-3751, 2011. 106. V.M. Gaspar, I.J. Correia, A. Sousa, F. Silva, C.M. Paquete, J.A. Queiroz, F. Sousa, “Nanoparticle mediated delivery of pure p53 supercoiled plasmid DNA for gene therapy”, Journal of Controlled Release, 156, 212-222, 2011. 107. I.E. Moreno, B.M. da Fonseca, M. Barroso, S. Costa, J.A. Queiroz, E. Gallardo, “Determination of piperazine-type stimulants in human urine by means of microextraction in packed sorbent and high performance liquid chromatography-diode array detection”, Journal of Pharmaceutical and Biomedical Analysis, 61, 93-99, 2012 108. D. Costa, J.A. Queiroz, M.G. Miguel, B. Lindman, “Swelling behavior of a new biocompatible plasmid DNA hydrogel”, Colloids and Surfaces B: Biointerfaces, 92, 106-112, 2012. 109. I.E. Moreno, B.M. da Fonseca, A.R. Magalhães, V.S. Geraldes, J.A. Queiroz, M. Barroso, S. Costa, E. Gallardo, “Rapid determination of piperazine-type stimulants in human urine by microextraction in packed sorbent after method optimization using a multivariate approach”, Journal of Chromatography A, 1222, 116-120, 2012. 110. F. Silva, J.A. Queiroz, F.C. Domingues, “Evaluating metabolic stress and plasmid stability in plasmid DNA production by Escherichia coli”, Biotechnology Advances, 30, 691-708, 2012. 111. B.M. da Fonseca, I.E. Moreno, A.R. Magalhães, M. Barroso, J.A. Queiroz, S. Ravara, J. Calheiros, E. Gallardo, “Determination of biomarkers of tobacco smoke exposure in oral fluid using solid-phase extraction and gas chromatography-tandem mass spectrometry”, Journal of Chromatography B, 15, 889-890, 116-122, 2012. 112. A. Palmeira-de-Oliveira, C. Gaspar, R. Palmeira-de-Oliveira, A. Silva-Dias, L. Salgueiro, C. Cavaleiro, C. Pina-Vaz, J. Martinez-de-Oliveira, J.A. Queiroz, A.G. Rodrigues, “The anti-Candida activity of Thymbra capitata essential oil: effect upon pre-formed biofilm”, Journal of Ethnopharmacology, 140, 379-383, 2012. 113. F. Silva, J.A. Queiroz, F.C. Domingues, “Plasmid DNA fermentation strategies: influence on plasmid stability and cell physiology”, Applied Microbiology and Biotechnology, 93, 2571-2580, 2012. 114. R. Martins, J.A. Queiroz, F. Sousa, “Histidine affinity chromatography-based methodology for the simultaneous isolation of Escherichia coli small and ribosomal RNA”, Biomedical Chromatography, 26, 781-788, 2012. 115. R. Silva, S. Ferreira, M.J. Bonifácio, J.M. Dias, J.A. Queiroz, L.A. Passarinha, “Optimization of fermentation conditions for the production of human soluble catecholO-methyltransferase by Escherichia coli using artificial neural network”, Journal of Biotechnology, 160, 161-168, 2012. 116. S. Ferreira, J. Santos, A. Duarte, A.P. Duarte, J.A. Queiroz, F.C. Domingues, “Screening of antimicrobial activity of Cistus ladanifer and Arbutus unedo extracts”, Natural Product Research, 26, 1558-1560, 2012. 117. M. Barroso, I.E. Moreno, B.M. da Fonseca, J.A. Queiroz, E. Gallardo, “Role of microextraction sampling procedures in forensic toxicology”, Bioanalysis, 4, 18051826, 2012. 118. J.C. Nunes, A.M. Morão, C. Nunes, M.T.P. Amorim, I.C. Escobar, J.A. Queiroz, “Plasmid DNA recovery from fermentation broths by a combined process of micro- and ultrafiltration: modeling and application”, Journal of Membrane Science, 415, 24-35, 2012. 119. D. Costa, A.J.M. Valente, M.G. Miguel, J.A. Queiroz, “Plasmid DNA Microgels for a therapeutical strategy combining the delivery of genes and anticancer drugs” Macromolecular Bioscience, 12, 1243-1252, 2012. 120. C. Cruz, A. Sousa, F. Sousa, J.A. Queiroz, “Study of the specific interaction between L-methionine chromatography support and nucleotides”, Journal of Chromatography B, 909, 1-5, 2012. 121. A.P. Gomes, J.F. Mano, J.A. Queiroz, I.C. Gouveia, “Layer-by-layer deposition of antibacterial polyelectrolytes on cotton fibres”, Journal of Polymers and the Environment, 20, 1084-1094, 2012. 122. A. Sousa, F. Sousa, J.A. Queiroz, "Advances in chromatographic supports for pharmaceutical-grade plasmid DNA purification", Journal of Separation Science, 35, 3046-3058, 2012. 123. R. Martins, C. Maia, J.A. Queiroz, F. Sousa, "A new strategy for RNA isolation from eukaryotic cells using arginine affinity chromatography", Journal of Separation Science, 35, 3217-3226, 2012. 124. V.M. Gaspar, C. Cruz, J.A. Queiroz, C. Pichon, I.J. Correia, F. Sousa, “Sensitive detection of peptide-minicircle DNA interactions by surface plasmon resonance”, Analytical Chemistry, 85, 2304-2311, 2013. 125. M. Rodrigues, G. Alves, M. Rocha, J.A. Queiroz, A. Falcão, “First liquid chromatographic method for the simultaneous determination of amiodarone and desethylamiodarone in human plasma using microextraction by packed sorbent (MEPS) as sample preparation procedure”, Journal of Chromatography B, 913-914, 90-97, 2013. 126. S. Ferreira, M.J. Fraqueza, J.A. Queiroz, F.C. Domingues, M. Oleastro, “Genetic diversity, antibiotic resistance and biofilm-forming ability of Arcobacter butzleri isolated from poultry and environment from a Portuguese slaughterhouse” International Journal of Food Microbiology, 162, 82-88, 2013. 127. C. Cruz, A. Sousa, F. Sousa, J.A. Queiroz, “Affinity analysis between immobilized L-arginine and plasmid isoforms provided by surface plasmon resonance”, Analytical Methods, 5, 1682-1686, 2013. 128. I. Moreno, B. da Fonseca, D. Oppolzer, A. Martinho, M. Barroso, A. Cruz, J.A. Queiroz, E. Gallardo, “Analysis of Salvinorin A in urine using microextraction in packed syringe and GC-MS/MS”, Bioanalysis, 5, 661-668, 2013. 129. C. Cruz, S.D. Santos, E.J. Cabrita, J.A. Queiroz, “Binding analysis between Lhistidine immobilized and oligonucleotides by SPR and NMR”, International Journal of Biological Macromolecules, 56, 175-180, 2013. 130. A.M. Goncalves, A.Q. Pedro, C. Maia, F. Sousa, J.A. Queiroz, L.A. Passarinha, “Pichia pastoris: a recombinant microfactory for antibodies and human membrane proteins, Journal of Microbiology and Biotechnology, 23, 587-601, 2013. 131. D. Oppolzer, I. Moreno, B. da Fonseca, L. Passarinha, M. Barroso, S. Costa, J.A. Queiroz, E. Gallardo, “Analytical approach to determine biogenic amines in urine using microextraction in packed syringe and liquid chromatography coupled to electrochemical detection”, Biomedical Chromatography, 27, 608-614, 2013. 132. C. Cruz, E. Cairrão, O. Lourenço, P.A. Almeida, I. Verde, J.A. Queiroz, “Polyazamacrocycles as potential antitumor agents for human prostate cancer cells”, Chemical Biology & Drug Design, 81, 517-526, 2013. 133. E. Mota, A. Sousa, U. Cernigoj, J.A. Queiroz, C.T. Tomaz, F. Sousa, “Rapid quantification of supercoiled plasmid deoxyribonucleic acid using a monolithic ion exchanger”, Journal of Chromatography A, 1291, 114-121, 2013. 134. F.M. Santos, A.Q. Pedro, R.F. Soares, R. Martins, M.J. Bonifácio, J.A. Queiroz, L.A. Passarinha, “Performance of hydrophobic interaction ligands for human membrane-bound catechol-O-methyltransferase purification”, Journal of Separation Science, 36, 1693-1702, 2013. 135. G. Alves, M. Rodrigues, A. Fortuna, A. Falcão, J.A. Queiroz, “A critical review of microextraction by packed sorbent as a sample preparation approach in drug bioanalysis”, Bioanalysis, 5, 1409-1442, 2013. 136. V.M. Gaspar, J.G. Marques, F. Sousa, R.O. Louro, J.A. Queiroz, I.J. Correia, “Biofunctionalized nanoparticles with pH-responsive and cell penetrating blocks for gene delivery”, Nanotechnology, 24, 275101, 2013. 137. B.M. da Fonseca, I.E. Moreno, M. Barroso, S. Costa, J.A. Queiroz, E. Gallardo, “Determination of seven selected antipsychotic drugs in human plasma using microextraction in packed sorbent and gas chromatography-tandem mass spectrometry”, Analytical and Bioanalytical Chemistry, 405, 3953-3963, 2013. 138. M. Rodrigues, G. Alves, A. Ferreira, J.A. Queiroz, A. Falcão, “A rapid HPLC method for the simultaneous determination of amiodarone and its major metabolite in rat plasma and tissues; a useful tool for pharmacokinetic studies”, Journal of Chromatographic Science, 51, 361-370, 2013. 139. T.R. Correia, B.P. Antunes, P.H. Castilho, J.C. Nunes, M.T.P. de Amorim, I.C. Escobar, J.A. Queiroz, I.J. Correia, A.M. Morão, “A bi-layer electrospun nanofiber membrane for plasmid DNA recovery from fermentation broths”, Separation and Purification Technology, 112, 20-25, 2013. 140. A. Palmeira-de-Oliveira, R. Palmeira-de-Oliveira, C. Gaspar, L. Salgueiro, C. Cavaleiro, J. Martinez-de-Oliveira, J.A. Queiroz, A.G. Rodrigues, “Association of Thymbra capitata essential oil and chitosan (TCCH hydrogel): a putative therapeutic tool for the treatment of vulvovaginal candidosis”, Flavour and Fragance Journal, 28, 354–359, 2013. 141. J.A. Queiroz, E. Gallardo, M. Barroso, “What are the recent advances in forensic oral fluid bioanalysis?”, Bioanalysis, 5, 2077-2079, 2013. 142. A.P. Gomes, J.F. Mano, J.A. Queiroz, I.C. Gouveia, “Layer-by-layer deposition of antimicrobial polymers on cellulosic fibers: a new strategy to develop bioactive textiles”, Polymers Advanced Technologies, 24, 1005-1010, 2013. 143. T. Matos, J.A. Queiroz, L. Bulow, “Binding and elution behavior of small deoxyribonucleic acid fragments on a strong anion-exchanger multimodal chromatography resin”, Journal of Chromatography A, 1302, 40-44, 2013. 144. R. Martins, J.A. Queiroz, F. Sousa, “New approach in RNA quantification using arginine-affinity chromatography: Potential application in eukaryotic and chemically synthesized RNA”, Analytical and Bioanalytical Chemistry, 405, 8849-8858, 2013. 145. M.S. Silva, V.C. Graça, L.V. Reis, P.F. Santos, P. Almeida, J.A. Queiroz, F. Sousa, “Protein purification by aminosquarylium cyanine dye-affinity chromatography”, Biomedical Chromatography, 27, 1671-1679, 2013. 146. A. Soares, J.A. Queiroz, F. Sousa, A. Sousa, “Purification of human papillomavirus 16 E6/E7 plasmid deoxyribonucleic acid-based vaccine using an arginine modified monolithic support”, Journal of Chromatography A, 1320, 72-79, 2013. 147. T. Matos, S. Senkbeil, A. Mendonça, J.A. Queiroz, J.P. Kutterc, L. Bulow, “Nucleic acid and protein extraction from electropermeabilized E. coli cells on a microfluidic chip”, Analyst, 138, 7347-7353, 2013. 148. F.F. Correia, F.M. Santos, A.Q. Pedro, M.J. Bonifácio, J.A. Queiroz, L.A. Passarinha, “Recovery of biological active catechol-O-methyltransferase isoforms from Q-sepharose”, Journal of Separation Science, 37, 20-29, 2014. 149. T. Matos, H.-O. Johansson, J.A. Queiroz, L. Bulow, “PCR DNA fragments purification using aqueous two-phase systems”, Separation and Purification Technology, 122, 144-148, 2014. 150. P. Pereira, A. Sousa, J.A. Queiroz, I. Correia, A. Figueiras, F. Sousa, “Purification of pre-miR-29 by arginine-affinity chromatography”, Journal of Chromatography B, 951-952, 16-23, 2014. 151. P. Pereira, A. Sousa, J.A. Queiroz, A. Figueiras, F. Sousa, “New approach for purification of pre-miR-29 using lysine-affinity chromatography”, Journal of Chromatography A, 1331, 129-132, 2014. 152. S. Ferreira, C. Júlio, J.A. Queiroz, F.C. Domingues, M. Oleastro, “Molecular diagnosis of Arcobacter and Campylobacter in diarrhoeal samples among Portuguese patients”, Diagnostic Microbiology and Infectious Disease, 78, 220-225, 2014. 153. A. Silva-Dias, A. Palmeira-de-Oliveira, I.M. Miranda, J. Branco, L. Cobrado, M. Monteiro-Soares, J.A. Queiroz, C. Pina-Vaz, A.G. Rodrigues, “Anti-biofilm activity of low-molecular weight chitosan hydrogel against Candida species”, Medical Microbiology and Immunology, 203, 25-33, 2014. 154. D. Costa, A.J.M. Valente, M.G. Miguel, J.A. Queiroz, “Plasmid DNA microgels for drug/gene co-delivery: a promising approach for cancer therapy”, Colloids and Surfaces A: Physicochemical and Engineering Aspects, 442, 181-190, 2014. 155. C. Cruz, R. Boto, P. Almeida, J.A. Queiroz, “NMR screening of new carbocyanine dyes as ligands for affinity chromatography”, Journal of Molecular Recognition, 27, 197-204, 2014. 156. T. Matos, J.A. Queiroz, L. Bulow, “Plasmid DNA purification using a multimodal chromatography resin”, Journal of Molecular Recognition, 27, 184-189, 2014. 157. V.M. Gaspar, C.J. Maia, J.A. Queiroz, C. Pichon, I.J. Correia, F. Sousa, “Improved minicircle DNA biosynthesis for gene therapy applications", Human Gene Therapy Methods, 25, 93-105, 2014. 158. D. Costa, A.J.M. Valente, M.G. Miguel, J.A. Queiroz, “Plasmid DNA hydrogels for biomedical applications”, Advances in Colloid and Interface Science, 205, 257-264, 2014. 159. J.C. Nunes, M.T.P. Amorim, I.C. Escobar, J.A. Queiroz, A.M. Morão, “Plasmid DNA/RNA separation by ultrafiltration: modeling and application study”, Journal of Membrane Science, 463, 1-10, 2014. 160. S. Ferreira, F. Silva, J.A. Queiroz, M. Oleastro, F.C. Domingues, “Resveratrol against Arcobacter butzleri and Arcobacter cryaerophilus: Activity and effect on cellular functions”, International Journal of Food Microbiology, 180, 62-68, 2014. 161. A. Afonso, P. Pereira, J.A. Queiroz, A. Sousa, F. Sousa, “Purification of pre-miR29 by a new O-phospho-L-tyrosine affinity chromatographic strategy optimized using design of experiments”, Journal of Chromatography A, 1343, 119-127, 2014. 162. G.M. Espírito Santo, A.Q. Pedro, D. Oppolzer, M.J. Bonifácio, J.A. Queiroz, F. Silva, L.A. Passarinha, “Development of fed-batch profiles for efficient biosynthesis of catechol-O-methyltransferase”, Biotechnology Reports, 3, 34-41, 2014. 163. A.Q. Pedro, R.F. Soares, D. Oppolzer, F.M. Santos, L.A. Rocha, A.M. Gonçalves, M.J. Bonifacio, J.A. Queiroz, E. Gallardo, L.A. Passarinha, “An improved HPLC method for quantification of metanephrine with coulometric detection”, Journal of Chromatography & Separation Techniques, 5, 217, 2014. 164. A.P. Gomes, J.F. Mano, J.A. Queiroz, I.C. Gouveia, “New biomaterial based on cotton with incorporated biomolecules”, Journal of Applied Polymer Science, 131, 40519, 1-9, 2014. 165. J. Santos, F. Sousa, J.A. Queiroz, D. Costa, “Rhodamine based plasmid DNA nanoparticles for mitochondrial gene therapy”, Colloids and Surfaces B: Biointerfaces, 121, 129-140, 2014. 166. C. Nunes, A. Sousa, J.C. Nunes, A.M. Morão, F. Sousa, J.A. Queiroz, “Supercoiled plasmid DNA purification by integrating membrane technology with a monolithic chromatography”, Journal of Separation Science, 37, 1229–1236, 2014. 167. J.F. Valente, A. Sousa, J.A. Queiroz, F. Sousa, “Selective purification of supercoiled p53-encoding pDNA with L-methionine-agarose matrix”, Analytical Biochemistry, 459, 61-69, 2014. 168. A.F. Jozala, L. Novaes, P. Mazzola, L. Oliveira-Nascimento, T. Penna, J.A. Teixeira, L. Passarinha, J.A. Queiroz, A.P. Júnior, “Low-cost purification of nisin from milk whey to a highly active product”, Food and Bioproducts Processing, in press. 169. E. Mota, F. Sousa, A. Sousa, J.A. Queiroz, C. Cruz, “Molecular recognition of oligonucleotides and plasmid DNA by L-methionine”, Journal of Molecular Recognition, in press. 170. A.S. Rocha, F.M. Santos, J.P. Monteiro, J.P. Castro-de-Sousa, J.A. Queiroz, C.T. Tomaz, L.P. Passarinha, “Trends in proteomic analysis of human vitreous humor samples”, Electrophoresis, in press. 171. R. Martins, J.A. Queiroz, F. Sousa, “Ribonucleic acid purification”, Journal of Chromatography A, in press. 172. D. Bicho, A. Sousa, F. Sousa, J.A. Queiroz, C.T. Tomaz., "Effect of chromatographic conditions and plasmid DNA size on the dynamic binding capacity of a monolithic support", Journal of Separation Science, in press. 173. A. Sousa, A.M. Almeida, U. Černigoj, F. Sousa, J.A. Queiroz, “Histamine monolith versatility to purify supercoiled plasmid DNA from Escherichia coli lysate”, Journal of Chromatography A, in press. 174. V.M. Gaspar, C. Gonçalves, D. Melo-Diogo, E.C. Costa, J.A. Queiroz, C. Pichon, F. Sousa, I.J. Correia, “Poly (2-ethyl-2-oxazoline)-PLA-g-PEI amphiphilic triblock micelles for co-delivery of minicircle DNA and chemotherapeutics”, Journal of Controlled Release, in press. 175. C. Cruz, A. Sousa, E. Mota, F. Sousa, J.A. Queiroz, “Quantitative analysis of histamine- and agmatine-DNA interactions using surface plasmon resonance”, International Journal of Biological Macromolecules, in press.