Curriculum vitae
Name: Maria de Fátima Silva Lopes
Education:
- PhD in Biochemistry at ITQB-Universidade Nova de Lisboa (December
1999)
- Graduated in Biochemistry (1987/1992), final score of 17 (in a scale of
1-20), at Faculdade de Ciências da Universidade de Lisboa.
Prior and current positions:
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IBET grant (1992/1994), to work on Project “Bactérias Lácticas de
Produtos Cárneos Portugueses: Isolamento, Caracterização e Produção
de Culturas de Arranque”, PBIC/C/AGR/1828/92;
1992-1994, Teatched Biochemistry , Escola Superior de Enfermagem
Artur Ravara
1994-1999, PhD student at ITQB, supervised by Teresa Crespo and
Manuel Carrondo. During this period, stayed for short periods, at
Stuttgart and Wageningen (NIZO) to peform par of the PhD work.
Worked as Quality Assurance at GLP Unit of IBET, from December 1999
to April 2004;
1999-2000, Teached Methabolism and Biochemistry of Food Products I
e II at Instituto Piaget, curso de Engenharia Alimentar
2000- 2003 Teached Biochemistry, Practical classes, Universidade
Católica, Faculdade de Engenharia, according to a protocol with ITQB,
Oeiras.
Post-doc position at IBET/FCUL, (PRAXIS XXI/BPD/22122/99); where
started working on antibiotic resistance and virulence of enterococci
from food products (2000 to 2003);
Since middle 2003 works as Investigadora Auxiliar at Laboratório
Associado IBET/ITQB/IGC, and is PI of the Laboratory “Stress by
antibiotics and virulence of enterococci”.
Projects as PI:
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POCTI/AGR/39371/2001 “Enterococci in Food: Antibiotics and Safety”.
POCI/CVT/59636/2004 "Antibiotic stress by vancomycin on enterococci: risk
assessment of food safety".
POCI/BIA-BCM/60643/2004 "Sense and sensibility: looking for the missing link
in lactic acid bacteria stress response pathways".
Assessment of virulence potential of enterococci food isolates. Cooperation
Luso/Francesa- Programa Pessoa 2006/2007.
POCTI/CVT/67270/2006. Virulence of food enterococci: adding new peaces
to the puzzling subject of enterococci as opportunistic pathogens.
ERA-PTG/0001/2006. Large scale screening of potential key factors involved
in the commensalism / virulence transition of Enterococcus faecalis.
Publications
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Teixeira N, Varahan S, Gorman MJ, Palmer KL, Zaidman-Remy A, Yokohata
R, Nakayama J, Hancock LE, Jacinto A, Gilmore MS, Lopes MFS. “Revisiting
the Fsr regulatory system of Enterococcus faecalis revels new quorumsensing partners and novel virulence factors in the Drosophila host model”,
submitted to PloS Pathogens.
Matos RC, Lapaque N, Rigottier-Gois L, Meylheuc T, Gonzalez-Zorn B,
Repoila F, Lopes MFS, Serror P. “Enterococcus faecalis prophage dynamics
and contributions 1 to pathogenic traits”. Submitted to PloS Genetics.
Abrantes, MC, Kok, J, Lopes, MFS (2012), ZntAEf mediates Enterococcus
faecalis defense against zinc overload, lysozyme and oxidative stress,
submitted to Metallomics.
Abrantes, MC, Kok, J, Lopes, MFS (2012), EfaR is a major regulator of
Enterococcus faecalis manganese transporters and influences processes
involved in host colonization and infection, Infection and Immunity, in
revision.
Braga, T, Pomba, C, Lopes, MFS (2012) High-level vancomycin resistant
Enterococcus faecium related to humans and pigs found in dust from pig
breeding facilities. Vet Microbiol, in press. (http://dx.doi.org/10.1016/
j.vetmic.2012.07.034.)
Gaspar, FB, Montero, N, Akary, E, Teixeira, N, Matos, R, Gonzalez-Zorn, B,
Crespo, MTB, Serror, P, Lopes, MFS (2012) Incongruence between the cps
type 2 genotype and host-related phenotypes of an Enterococcus faecalis
food isolate. IJFM, in press. (doi:10.1016/j.ijfoodmicro.2012.07.006).
Teixeira, N, Santos, S, Marujo, P, Yokohata, R, Iyer, V, Nakayama, J,
Hancock, LE, Serror, P, Lopes, MFS (2012) Incongruent gelatinase
phenotype and genotype in Enterococcus faecalis is due to shutting off the
ability to respond to the GBAP quorum-sensing signal. Microbiology, 158:
519-528.
Proença, D, Fernandes, S, Leandro, C, Silva, FA, Santos, S, Lopes, F, Mato,
R, Cavaco-Silva, P, Pimentel, M, São-José, C (2012) Phage endolysins with a
broad antimicrobial activity against Enterococcus faecalis clinical Strains.
Microb Drug Resist, 18(3):322-32.
Pinto, D, Ruivo, M, Vandamme, P, Lopes, MFS (2012) Hospital-adapted
clonal complex 17 Enterococcus faecium found among sand enterococcal
isolates. Journal of Environmental Protection, 3: 74-82.
Rigottier-Gois, L, Alberto, A, Houel, A, Taly, J-F, Palcy, P, Manso, J, Pinto,
D, Matos, R, Carrilero, L, Montero, N, Tariq, M, Karsens, H, Repp, C, Kropec,
A, Budin-Verneuil, A, Benachour, A, Sauvageot, N, Bizzini, A, Gilmore, MS,
Bessieres, P, Kok, J, Huebner, J, Lopes, F, Gonzalez-Zorn, B, Hartke, B,
Serror, P (2011) Large-scale screening of a targeted Enterococcus faecalis
mutant library identifies envelop fitness factors, PlosOne, 6:12, e29023.
Abrantes M, Lopes, MFS, Kok J. (2011), Impact of manganese, copper and
zinc ions on the transcriptome of the nosocomial pathogen Enterococcus
faecalis V583, PlosOne, 6: 10, e26519.
Ribeiro T, Santos S, Marques MIM, Gilmore M, Lopes MFS. (2011)
Identification of a new gene, vanV, in vanB operons of Enterococcus
faecalis, IJAA, 37:554-557.
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Braga TM, Marujo PE, Pomba C, Lopes MFS. (2011), Involvement, and
dissemination, of the enterococcal small multidrug resistance transporter
QacZ in resistance to quaternary ammonium compounds, JAC, 66(2):283286.
Gaspar, F, Teixeira, N, Rigottier-Gois, L, Marujo, P, Nielsen-LeRoux, Crespo,
MTB, Lopes, MFS, Serror, P. (2009), Virulence of Enterococcus faecalis dairy
strains in an insect model: the role of fsrB and gelE, Microbiology 155:35643571.
Gaspar, F, Crespo, MTB, Lopes, MFS (2009), Proposal for a reliable
enterococcal cytolysin production assay avoiding apparent incongruence
between phenotype and genotype, J Med Microbiol, 58:1122-1124.
Matos, R, Pinto, V, Ruivo, M, Lopes, MFS (2009), Study on the dissemination
of the bcrABDR cluster in Enterococcus spp. reveals that the BcrAB
transporter is sufficient to confer high-level bacitracin resistance, Int J
Antimicrob Agents 34:142-147.
Marques, J, Braga, TM, Paz, FAA, Santos, TM, Lopes, MFS, Braga, SS (2009),
Cyclodextrins improve the antimicrobial activity of the chloride salt of
Ruthenium(II) chloro-phenanthrolinetrithiacyclononane, BioMetals 22:541556.
Ribeiro, T, Pinto, V, Gaspar, F, Lopes, MFS (2008), Enterococcus hirae
causing wound infections in a hospital, JCCM,3:150-152.
Corsetti A, Settanni L, Braga TM, Lopes MFS, Suzzi G (2008), An
investigation of the bacteriocinogenic potential of lactic acid
bacteriaassociated with wheat (Triticum durum) kernels andnonconventional flours, LWT-Food Science and Technology. 41:1173-1182.
Ribeiro, T., Abrantes, M., Lopes, M.F.S., Tenreiro, R., Barreto Crespo, M.T.
(2007) "Vancomycin susceptible dairy and clinical enterococcal isolates
carry vanA and vanB genes", Int J Food Microbiol, 113: 289-295.
Lopes, M.F.S., Simões, A.P., Tenreiro, R., Marques, J.J.F., Barreto Crespo,
M.T. (2006) "Activity and expression of a virulence factor, gelatinase, in
dairy enterococci", Int J Food Microbiol, 112 (3): 208-214.
Zuber, B, Haenni, M, Ribeiro, T, Minnig, K, Lopes, F, Moreillon, P,
Dubochet, J (2006) "Granular layer in the periplasmic space of Grampositive bacteria and fine structures of Enterococcus gallinarum and
Streptococcus gordonii septa revealed by cryo-electron microscopy of
vitreous sections", J Bacteriol, 188: 6652-6660.
Lopes, M.F.S., Ribeiro, T., Abrantes, M., Marques, J.J.F., Tenreiro, R.,
Barreto Crespo, M.T. (2005) "Antimicrobial Resistance Profiles of Dairy and
Clinical Isolates and Type Strains of Enterococci", Int. J. Food Microbiol.,
103: 191-198.
Semedo, T., Santos, M. A., Martins, P., Lopes, M. F. S., Marques, J. J. F.,
Tenreiro, R., Barreto Crespo, M. T. (2003) "Comparative study using type
strains and clinical and food isolates to examine hemolytic activity and
occurrence of the cyl operon in enterococci" , J. Cli Microbiol., 41: 25692576.
Semedo, T., Santos, M. A., Lopes, M. F. S., Marques, J. J. F., Barreto
Crespo, M. T., Tenreiro, R. (2003) "Virulence factors in food, clinical and
reference enterococci: a common trait in the genus", System. Appl.
Microbiol., 26:13-22.
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Lopes, M. F. S., Ribeiro, T., Martins, M. P.,Tenreiro, R., Barreto Crespo, M.
T. (2003) “Gentamicin resistance in dairy and clinical enterococcal isolates
and in reference strains", J. Antimicrob. Chemother., 52:214-219.
Lopes, M.F.S., Leitão, A.L., Regalla, M., Marques, J.J.F., Carrondo, M.J.T.,
Barreto Crespo, M.T. (2002) “Characterization of a highly thermostable
extracellular lipase from Lactobacillus plantarum”, Int. J. Food Microbiol.,
76: 107-115.
Lopes, M.F.S., Pereira, C.I., Rodrigues, F.M.S., Martins, M.P., Mimoso, M.C.,
Barros, T.C., Figueiredo Marques, J.J., Tenreiro, R.P., Almeida, J.S.,
Barreto crespo, M.T. (1999) “Registered Designation of Origin Area of
Fermented Food Products Defined by Microbial Phenotypes and Artificial
Neural Networks”, Appl. Environ. Microbiol.,65: 4484-4489.
Lopes, M.F.S., Leitão, A.L., Marques, J.J.F., Carrondo, M.J.T., Barreto
Crespo, M.T. (1999) “Processing of extracellular lipase of Lactobacillus
plantarum: involvement of a metalloprotease”, FEMS Microbiol. Letters,
176(2): 483-487.
Lopes, M.F.S, Cunha, A.E., Clemente, J.J., Carrondo, M.J.T., Barreto
Crespo, M.T. (1999) “Influence of environmental factors on lipase
production by Lactobacillus plantarum”, Appl. Microbiol. Biotechnol., 51:
249-254.
Gaspar, J., Rodrigues, A., Laires, A., Silva, F, Costa, S., Monteiro, M.J.,
Monteiro, C., Rueff, J. (1994) “On the mechanisms of genotoxicity and
metabolism of quercetin”, Mutagenesis, 9(5): 445-449
Book Chapters
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Lopes, MFS, Carrilero, L, Gonzalez-Zorn, B (2012) Post-genomic strategies
towards evaluation of pathogenicity potential, Chapter 14, Enterococcus and
Safety, T Semedo-Lemsaddek, T Crespo and R Tenreiro (Eds), Nova Science
Publishers, ISBN 978-1-61470-569-7.
Oral Communications:
M.F.S. Lopes, A.P. Simões, R. Tenreiro, J.J.F. Marques, M.T.B. Crespo, “Activity
and expression of a virulence factor, gelatinase, in dairy enterococci”,
FoodMicro2004, 12-16/9, 2004, Portoroz, Eslovénia.
Ribeiro T, Abrantes M, Lopes MFS, Tenreiro R, Crespo MTB “Vancomycin resistance
in dairy and clinical enterococcal isolates from Portugal: proof of dissemination of
Tn1546”, FoodMicro2004, 12-16/9, 2004, Portoroz, Eslovénia.
Ruivo M, Ribeiro T, Abrantes M, Lopes, MFS “Evidence for cross-transmission of
vanA-carrying Enterococcus faecalis between dairy food products and humans”,
FoodMicr0 2006, 28/8/2/9 de 2006, Bolonha, Itália.
Maria de Fatima Silva Lopes, "Enterococcal reservoirs: antibiotic resistance and
typing". This was a personal communication by invitation at INRA, Jouy-en-Josas,
May,
2007.
Maria de Fatima Silva Lopes, "Antibiotic resistance and virulence: common traits
in the genus Enterococcus". This was a personal communication by invitation at
Groningen Universiy, March, 2008.
Teresa M. Braga, Constança Pomba and Maria de Fátima Silva Lopes. Enterococci
isolated from Portuguese pig facilities, a cause of concern? 2009, CESAR meeting in
Croatia.
Maria de Fatima Silva Lopes, Recent findings on antibiotic resistance and
virulence of an old companion, ITQB, December, 2010.
Renata Matos, Bruno Gonzalez-Zorn, Maria de Fátima Lopes, Pascale Serror, The
prophage pool of Enterococcus faecalis V583, July 2011, 6th International
Conference on Gram-positive Microorganisms, Italy.
Posters
Matos, R, Cotes-Perez, N, Navickas, A, Meyleuc, T, Repoila, F, Rigottier-Gois, L,
Gonzalez-Zorn, B, Lopes, MFS, Serror, P. “Prophage-crosstalk and prophage
associated citotoxicity in Enterococcus faecalis V583”, June 2012, 2nd edition
Conference Virus of Microbes, Brussels.
Proença, D, Pimentel, M, Lopes, F, Mato, R, São-José, C “Phage endolysisns with
antimicrobial activity against enterococcal clinical isolates”, Microbial viruses:
Genomics, Evolution and Applications in Ecology, Biotechnology and Medicine,
Queen's University Belfast, Irlanda do Norte, 19-20 de Abril de 2011.
Proença, D, Pimentel, M, Lopes, F, Mato, R, Garcis, M, São-José, C “Cell-wall lytic
activity of two endolysins targeting enterococcal clinical strains”: The great Wall
Symposium, Hotel Villa Galé de Cascais, Lisboa, 28-30 de Setembro de 2011.
Teixeira N., Zaidman-Rémy A., Jacinto A., Lopes M. F. S.; Drosophila melanogaster:
A model to study E. faecalis virulence; in DrosTuga 2011; ; IGC - Oeiras - Portugal;
December 2011.
Teixeira, N.; Lyer V.; Varahan S.; Hancock, LE; Palmer K., Gilmore MS.; Lopes, M.
F. S.; E. faecalis Fsr quorum sensing system is involved in regulation of LrgAB, in
FEMS- 4th Congress of European Microbiologists; Geneva-Switzerland June 26-30
2011.
Teixeira, N. ; Zaidman-Rémy A.; Jacinto A. and Lopes M. F.S.; Drosophila
melanogaster: a model to study E. faecalis virulence; in 22nd Drosophila Research
Conference; Fundação Gulbenkian -Lisbon-Portugal; 21 to 24 September 2011.
N. Teixeira, P. Marujo, F. Gaspar, M.D.F. Silva Lopes. 2009. A truncated sensor
kinase is responsible for the lack of gelatinase activity in a strain carrying the
complete fsr and gelE-sprE operons. FEMS Meeting in Gottenborg.
ASM General Meeting in San Diego:
- Frédéric Gaspar, Teresa Crespo, and Fátima Lopes. 2009. Role of the LuxS/AI-2
System in the Metabolism and Virulence of Enterococcus faecalis.
- Abrantes M.C, Kok, J and Lopes, M. de F.2009. Metal stress responses in
Enterococcus faecalis.
- Tânia C. Ribeiro, Michael S. Gilmore, Maria F. S. Lopes. 2009. vanRS and vanB
contribute to vancomycin adaptation even in a susceptible genetic background.
Abrantes, MC, Lopes, MFS, Kok, J "Influence of metal stress on Enterococcus
faecalis gene expression", IUMS August 2008, Istambul.
Ribeiro, T, Gilmore, M, Lopes, MFS "Enterococcus faecalis vanRB regulon:
identification of new putative VanR targets", IUMS August 2008, Istambul.
Braga, TM, Pomba, C, Lopes, MFS "The role of EFA0010 in Enterococcus resistance
to QAC", IUMS August 2008, Istambul.
Teixeira, N, Marujo, P, Alberti, A, Rigottier-Gois, L, Serror, P, Lopes, MFS
"Truncated FsrC may be the reason for the absence of a gelatinase phenotype in
the presence of fsrgelE operons", IUMS August 2008, Istambul.
Ruivo, M, Marujo, P, Lopes, MFS "Genomic comparison of two E. faecalis strains
from different environments by Supression Subtractive Hybridization", IUMS 2008
Istambul, August.
Pinto, D, Lopes, MFS "Characterization of enterococci isolated from sand", ASM
Meeting Boston, 2008.
Ribeiro, T, Lopes, MFS, Gilmore, M "Transcriptomic profile of E. faecalis to
therapeutic dose of vancomycin", ASM Meeting Boston, 2008.
Matos, R, Pinto, V, Ruivo, M, Lopes, MFS "Dissemination of bacitracin resistance in
enterococcal isolates", ASM Meeting Boston 2008.
Pomba C, Delgado M,Ribeiro T, Crespo T, Lopes MFS, Duarte Correia JH. High-level
gentamicin resistance in clinical enterococcal isolates of companion animals in
Portugal. Poster presented at the European Congress on Clinical Microbiology and
Infectious Diseases (ECCMID),1 - 4 April, Nice, France.
Tânia Ribeiro, Marta Abrantes, MFS Lopes. Susceptible and intermediate
enterococcal strains are able to persist in high vancomycin concentrations. This
poster will be presented in FoodMicro2006, 29 Aug-2 Spt, Bolonha, Italy.
Neuza Teixeira, Marta Ruivo, Tânia Ribeiro, Maria F. S. Lopes. Enterococcus hirae
CELLS ARE ABLE TO DIVIDE, METABOLIZE GLUCOSE AND REMAIN CULTIVABLE AFTER
INCUBATION IN WATER. This poster will be presented in FoodMicro2006, 29 Aug-2
Spt,Bolonha, Italy.
V Pinto, MF Silva Lopes, MTB Crespo "Bacitracin resistance in Enterococcus sp
isoated from water, hospitals and clinics, veterinary and dairy products in
Portugal", Book of abstracts, 8th Symposium on Lactic Acid Bacteria, abstract
H037, 2005
T Ribeiro, M Ruivo, MF Silva Lopes "Dairy and clinical enterococci are vancomycin
resistance disseminators", Book of abstracts, 8th Symposium on Lactic Acid
Bacteria, abstract H034, 2005.
T Ribeiro, MF Silva Lopes "Post-antibiotic effect of vancomycin susceptible,
intermediate and resistant clinical and dairy enterococcal isolates", Book of
abstracts, 8th Symposium on Lactic Acid Bacteria, abstract H036, 2005.
Semedo, T, M. F. Lopes, J. J. Figueiredo Marques, M. T. Barreto Crespo, R.
Tenreiro. 2003. “Are virulence factors a common trit in the genus Enterococcus?.
Micro’2003, Tomar.
T. Semedo, F. Lopes, M.T. Barreto Crespo, R.P. Tenreiro. 2001. “Isolados clínicos
versus ambientais: pesquisa de factores de virulência em enterococos”.
Micro’2001, Póvoa do Varzim, Portugal
T. Ribeiro, M. F. Silva Lopes, M. P. Martins, R. Tenreiro, M. T. Barreto Crespo
"Gentamicin resistance in dairy and clinical enterococcal isolates and in reference
strains", MICRO 2003, 29/11-2/12, 2003, Tomar.
M. F. Silva Lopes, T. Ribeiro, M. Abrantes, J. J. F. Marques, R. Tenreiro, M. T.
Barreto Crespo "Antimicrobial resistance profiles of dairy and clinical isolates and
type strains of enterococcus", MICRO 2003, 29/11-2/12, 2003, Tomar.
T. Ribeiro,M. F. Silva Lopes, A. Eugénio, M. Abrantes, R. Tenreiro, M. T. Barreto
Crespo. "Transfer of antibiotic resistance genes in dairy enterococci" 1st FEMS
Congress of European Microbiologists, 29/6-3/7, 2003, Eslovénia.
A.
P. Simões, M. F. Silva Lopes, R. Tenreiro, J. J. F. Marques, M. T. Barreto
Crespo "Activity and production of a virulence factor, gelatinase, in dairy
enterococci", MICRO 2003, 29/11-2/12, 2003, Tomar.
M. F. Silva Lopes, T. Ribeiro, R. Tenreiro, M. T. Barreto Crespo. "Antibiotic
resistance in dairy enterococci: what should we fear?" 1st FEMS Congress of
European Microbiologists, 29/6-3/7, 2003, Eslovénia.
Lopes, M. F. S., Ribeiro, T., Eugénio, A., Tenreiro, R. P., Barreto Crespo, M. T.
“Dissemination of Tn917 and ermB in dairy enterococci”,7th Symposium of Lactic
Acid Bacteria- Genetics, Metabolism and Applications, Egmond aan Zee, Holanda,
1-5 de Setembro, 2002.
Eugénio, A., Lopes, M. F. S., Tenreiro, R. P., Barreto Crespo, M. T. “Plasmid
associated antibiotic resistance in food enterococci”, Enterococci in foodsfunctional and safety aspects, Berlim, Alemanha, 30 e 31 de Maio, 2002.
Ribeiro, T., Lopes, M. F. S., Martins, M. P., Barreto Crespo, M. T. “Characterisation
of gentamicin resistance in dairy and clinical enterococci”, Enterococci in foodsfunctional and safety aspects, Berlim, Alemanha, 30 e 31 de Maio, 2002.
Lopes, M. F. S., Alves, P., Tenreiro, R. P., Barreto Crespo, M. T. “Dissemination of
Tn917 and ermB in dairy enterococci”, Enterococci in foods- functional and safety
aspects, Berlim, Alemanha, 30 e 31 de Maio, 2002.
Lopes, M. F. S., Tenreiro, R. P., Barreto Crespo, M. T. “Dissemination of Tn917 and
ermB in dairy enterococci”, Congresso Nacional de Microbiologia Micro 2001, Póvoa
do Varzim, 5 a 8 de Dezembro, 2001.
Ribeiro, T., Lopes, M. F. S., Martins, M. P., Barreto Crespo, M. T. “Characterisation
of gentamicin resistance in dairy and clinical enterococci”, Congresso Nacional de
Microbiologia Micro 2001, Póvoa do Varzim, 5 a 8 de Dezembro, 2001.
Lopes, M.F.S., Leitão, A.L., Barreto Crespo, M.T., “Effect of Proteases on the
Extracellular Lipolytic Activity of Lactobacillus plantarum”, Congresso Nacional de
Microbiologia, Hotel dos Templários, Tomar, 29 Novembro - 1 Dezembro 1997.
Lopes, M.F.S., Rodrigues, F.M.S, Barreto crespo, M.T., “Flavour and the Lipolytic
Activity of Lactobacillus”, 5th Symposium on Lactic Acid Bacteria: Genetics
Metabolism and Applications, Veldhoven, Holanda, 8 a 12 Setembro 1996.
Pereira, C.I.; Lopes, M.F.S.; Rodrigues, F.M.S.; Almeida, J.S.; Marques, J.J.F.;
Barreto Crespo, M.T., “Evaluation of the Diversity of Lactobacillus Strains Present
in Portuguese Dry Fermented Sausages”, 5th Symposium on Lactic Acid Bacteria:
Genetics Metabolism and Applications, Veldhoven, Holanda, 8 a 12 Setembro 1996.
Rodrigues, F.S.; Pereira, C.I.; Silva, M.F.; Figueiredo Marques, J.J.; Crespo, M.T.B.,
“Microbial Diversity in Dry Smoked “chouriço” Sausages”, International Symposium
exploration of Microbial Diversity - Ecological Basis and Biotechnological Utility”,
Goslar, Alemanha, 12 a 15 Junho 1995.
Pereira, C.I.; Silva, M.F.; Spínola Rodrigues, F.M.; Mikami, A.; Barreto Crespo,
M.T., “A First Approach to the Microbial Characterization of Traditionally Produced
“Chouriço””, II Congresso Ibérico de biotecnologia, Vilamoura, Portugal, 2 a 4
Outubro 1994.
Silva, M.F.; Pereira, C.I.; Spínola Rodrigues, M.F.; Barreto crespo, M.T., “Lipolytic
Activity of Lactobacillus Isolated from Traditionally Fermented Food Products”, II
Congresso Ibérico de biotecnologia, Vilamoura, Portugal, 2 a 4 Outubro 1994.
Pereira, C.I.; Silva, M.F.; Crespo, M.T., “Characterization of Lactobacillus Isolated
from Portuguese Dry Sausages for Production of a Starter Culture”, International
Congress of Bacteriology and Applied Microbiology Division, Praga, República
Checa, 2 a 8 Julho 1994.
Mikami, A.; Pereira, C.; Silva, M.F.; Crespo, M.T.; Marques, F.”Study of the Lactic
Acid bacteria Present in Traditional Portuguese Meat Products”, 4th Symposium
Lactic Acid Bacteria, Genetics, Metabolism and Applications, Noordwijkerhout,
Holand, 5 a 9 Setembro 1993.
Gaspar, J., Laires, A., Costa, S., Silva, M.F., Monteiro, C., Rueff, J. “Aspectos
Radicalares da Genotoxicidade de Flavonóis”, I Congresso Nacional de Radicais de
Oxigénio, Lisboa, Junho de 1993
Gaspar, J., Silva, M.F., Laires, A., Rueff, J. “Free Radicals and the Mechanisms of
Genetic Lesion of the Food Mutagen Quercetin”, Proc. 6th International Conference
on Environmental Mutagens, em Melbourne, Austrália, Fevereiro de 1993.
Awards
Grant from ESCMID, to attend to the Conference on Enterococci: from Animals to
Man, 18 - 20 November 2009, Barcelona, Spain, where our work from the Era-Net
project was presented by Pascale Serror: Post-genomic strategies to identify
factors relevant for the in vivo lifestyle of E. faecalis.
Colaborations (present and past)
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Pascale Serror , Unité de Recherche Laitiere et Genetique Appliquee, INRA,
Jouy En Josas, France
Michael Gilmore, The Schepens Eye Research Institute, Harvard Medical
School
Jan Kok, University of Groningen
Jiro Nakayama, Japan
Lynn E. Hancock, USA
António Jacinto, CEDOC, Universidade Nova de Lisboa
Francisco Dionísio, FCUL, Lisboa
Teresa Crespo, IBET/ITQB
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Constança Pomba, Department of Clinics, Faculty of Veterinary Medicine,
Universidade Técnica de Lisboa
Bruno Gonzales-Zorn,
Departamento de Sanidad Animal, Facultad de
Veterinaria, Universidad Complutense de Madrid
Axel Hartke, Lab of Environmental Microbiology, IBFA, University of Caen,
France
Andreas Meinke, Intercell, Austria
Jacques Dubochet and Benoit Zuber, Laboratory of Ultrastructural Analysis,
Lausanne
Johannes Huebner, Infectious Disease Research, Department of Medicine,
University Hospital Freiburg
Rob Willems, Utrecht
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Susana Santos Braga, CICECO, Universidade de Aveiro
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Thesis supervision
Finished PhD Thesis:
- Studies on resistance and response to vancomycin in Enterococcus
faecalis: a last resort antibiotic. Student: Tânia Ribeiro. Finished in 2011.
- Enterococcus and biocides: mechanisms of tolerance and selection for
vancomycin resistance. Student: Teresa Braga. Finished in 2011.
- Non-clinical isolates bring new findings on enterococcal virulence
Student: Frédéric Bustos Gaspar. Finished in 2012.
- Precious transition metals: The importance of Zn2+, Mn2+ and Cu2+ for
the human pathogen Enterococcus faecalis Student: Marta Coelho
Abrantes. Finished in 2012.
On-going PhD thesis:
 Functional analysis of fsr-gelE-sprE role in enterococcal virulence”. Student:
Neuza Teixeira.
 Influence of prophage genes in virulence and fitness of Enterococcus
faecalis. Student: Renata matos. (Co-supervision with Pascale Serror).
 Reforço da resistência a antibióticos e da virulência em bactérias, através de
transferência horizontal de genes. Student: Iolanda Lopes Domingues. (Cosupervision with Francisco Dionísio, main supervisor).
Master Students:
- Resistência à vancomicina em enterococos: uma avaliação global de risco.
Mestrado em Microbiologia Clínica, Faculdade de Medicina, Universidade de Lisboa.
Mestranda Tânia Ribeiro.
- Avaliação da disseminação de resistências a antibióticos em enterococos isolados
em diferentes habitats: clínicos, veterinários, ambientais e alimentares. Mestrado
em Microbiologia Médica. Universidade Nova de Lisboa. Mestranda Vera Valadão
Pinto.
- Estudo da resistência à bacitracina em isolados de enterococos. Faculdade de
Ciências da Universidade de Lisboa. Mestranda Renata Matos.
- Estudo de resistência a antibióticos em estirpes de enterococos isolados de
areias. Faculdade de Ciências da Universidade de Lisboa. Mestranda Daniela Pinto.
- Estudo genético e fenotípico dos operões fsr e gelE-sprE em diferentes estripes
de Enterococcus, Mestrado em Microbiologia Clínica, Faculdade de Medicina,
Universidade de Lisboa, Neuza Teixeira.
- Estudo da disseminação de Enterococcus faecalis vanA+ em diferentes ambientes,
Mestrado em Microbiologia Clínica, Faculdade de Medicina, Universidade de Lisboa,
Marta Ruivo.
- Estudo do papel de proteínas hipotéticas na tolerância à vancomicina em
Enterococcus faecalis, Mestrado em Genética Molecular e Biomedicina da
Faculdade
de
Ciências
e
Tecnologia
da
Universidade Nova de Lisboa, Sofia Santos
Undergraduate students:
- Caracterização da Resistência à gentamicina em Enterococcus lácteos e clínicos.
Estágio da Licenciatura em Bioquímica pela Faculdade de Ciências da Universidade
de Lisboa, ano lectivo de 2000-2001. Licencianda Tânia Ribeiro.
- Resistência a antibióticos em enterococos associada a plasmídeos.Estágio da
Licenciatura em Bioquímica pela Faculdade de Ciências da Universidade de Lisboa,
ano lectivo de 2001-2002. Licencianda Ana Eugénio.
- Estudo da resistência à vancomicina em enterococos lácteos e clínicos.Estágio da
Licenciatura em Bioquímica pela Faculdade de Ciências da Universidade de Lisboa,
ano lectivo de 2002-2003. Licencianda Marta Abrantes.
- Produção de gelatinase associada à cultura em laboratório. Estágio da
Licenciatura em Bioquímica pela Faculdade de Ciências da Universidade de Lisboa,
ano lectivo de 2002-2003. Licencianda Patrícia Simões.
- Estudo da resistência à vancomicina en enterococos clínicos e lácteos. Estágio de
Licenciatura em Engenharia Biotecnológica pela Universidade Lusófona de
Humanidades e Tecnologias, ano lectivo de 2003-2004. Licencianda Marta Ruivo.
- Estudo proteico e genético das proteínas envolvidas na divisão celular de
Enterococcus sp.. Licenciatura em Engenharia Biotecnológica pela Universidade
Lusófona de Humanidades e Tecnologias, ano lectivo de 2004-2005. Licencianda
Neuza Teixeira.