Proteomics Lab@UA
Head of Proteomics Lab: Rui Vitorino, Auxiliary Researcher
Mass Spectrometry Center (http://masspec.web.ua.pt/)
QOPNA- http://www.ua.pt/qopna/
Department of Chemistry – University of Aveiro
Tel: 234370695 ext. 23507
Email: [email protected]
RUI VITORINO
Rui Vitorino is graduated in food biochemistry and got his Ph.D. in Biochemistry at the
University of Aveiro (Portugal). Following post-doctoral work, he held a position at the Aveiro
in 2008 as Auxiliary Researcher.
Research by Vitorino and coworkers is centered on the improvement, development and
application of proteomics technologies to biomedicine with major focus in salivary proteomics:
(i) basic salivary research aiming the identification and characterization of the salivary
components;
(ii) impact of pathophysiological conditions on salivary proteome for the identification of
biomarkers and/or therapeutic targets.
Rui Vitorino has published over 78 peer-reviewed papers in scientific journals, 5 book chapters
and 1 book. He is also interested in promoting proteomic research and education through
organization of workshops and training courses.
LAB MEMBERS
Post-Doctoral Researchers
José Alexandre Ferreira
“The role of mitochondria in the energy metabolism of tumor cells: a proteome perspective”
SFRH/BPD/66288/2009
Email: [email protected]
Ph. D. Students
Virginia Maria Carvalhais
“Characterization of dormancy in S. epidermidis biofilms and its impact on resistance”
SFRH/BD/78235/2011
Email: [email protected]
Researcher Fellowships
Rui Silva
“Synthesis and functionalization of magnetic nano-beads for protease capture”
Email. [email protected]
João Costa
“Characterization of proteases in saliva using magnetic nano-beads platform”
Email. [email protected]
Former
Ph. D. Students
Armando Cerejo Caseiro
“Salivary proteomics of diabetes mellitus type 1”
SFRH/BD/30124/2006
Email. [email protected]
Ms. Students
Telma Martins
“Study of PAH using a proteomic approach”.
Rui Pedro Silva
“Nanoparticles for protease characterization”.
João Pedro Marques
“Proteomic approach to the identification of Plasmodium falciparum determinants of drug
resistance”.
Matilde Melo
“Identification of “Protein X” binding partners under oxidative stress conditions: finding new
targets for Parkinson’s Disease”
Patrícia de Sousa Pereira
“Characterization of mammal salivary peptides”
Neuza Faustino
“Salivary peptides: a phylogenetic approach”.
Miguel Costa Ribeiro
“Proteolytic cascades regulated by estrogen β receptor”.
Hugo Branco Cardoso
“Epigenetic markers in HC11 cells”.
Ana Lúcia Teixeira
“Viral peptidomics”.
SELECTED PUBLICATIONS
Salivary Proteome and Peptidome Profiling in Type 1 Diabetes Mellitus Using a
Quantitative Approach. Caseiro A, Ferreira R, Padrão A, Quintaneiro C, Pereira A,
Marinheiro R, Vitorino R, Amado F. J Proteome Res. 2013; 12 (4):1700–1709
An evolutionary perspective of mammal salivary peptide families: Cystatins, histatins,
statherin and PRPs. de Sousa-Pereira P, Amado F, Abrantes J, Ferreira R, Esteves PJ,
Vitorino R. Arch Oral Biol. 2013;58(5):451-8.
One decade of salivary proteomics: current approaches and outstanding challenges.
Amado F, Ferreira R, Vitorino R. Clin Biochem. 2013;46(6):506-17.
iTRAQ-based Quantitative proteomic analysis of submandibular glands from rats with STZinduced hyperglycemia. Alves RM, Vitorino R, Padrão AI, Moreira-Gonçalves D, Duarte JA,
Ferreira R, Amado F. J Biochem, 2013;153(2):209-20
Characterization of thymosin β4 in mammals' saliva. de Sousa-Pereira P, Abrantes J, Colaço
B, Castagnola M, Amado F, Esteves PJ, Vitorino R. Peptides. 2012;40C:1-7.
Protease profiling of different biofluids in type 1 diabetes mellitus. Caseiro A, Ferreira R,
Quintaneiro C, Pereira A, Marinheiro R, Vitorino R, Amado F. Clin Biochem 2012 45(18):
1613-9
Proteome-base biomarkers in Diabetes Mellitus: from discovery to clinical applications.
Padrão AI, Ferreira R, Vitorino R, Amado F. Proteomics Clin Applic. 2012; 6(9-10):447-66.
Toward a standardized saliva proteome analysis methodology. Vitorino R, Guedes S,
Manadas B, Ferreira R, Amado F. J Proteomics 2012 75(17):5140-65.
Evaluation of different extraction procedures for salivary peptide analysis. Vitorino R,
Barros AS, Caseiro A, Ferreira R, Amado F. Talanta 2012 94:209-15.
Salivary peptidome in type 1 diabetes mellitus. Caseiro A, Vitorino R, Barros AS, Ferreira R,
Calheiros-Lobo MJ, Carvalho D, Duarte JA, Amado F. Biomed Chromatogr. 2012
May;26(5):571-82
Synthesis and optimization of lectin functionalized nanoprobes for the selective recovery
of glycoproteins from human body fluids. Ferreira JA, Daniel-da-Silva AL, Alves RM, Duarte
D, Vieira I, Santos LL, Vitorino R, Amado F. Anal Chem. 2011 Sep 15;83(18):7035-43.
Finding new posttranslational modifications in salivary proline-rich proteins. Vitorino R,
Alves R, Barros A, Caseiro A, Ferreira R, Lobo MC, Bastos A, Duarte J, Carvalho D, Santos LL,
Amado F. Proteomics Clin Applic. 2011; (5) 3-4: 197 - 197.
Reactivity of Human Salivary Proteins Families Toward Food Polyphenols. Soares S,
Vitorino R, Osório H, Fernandes A, Venâncio A, Mateus N, Amado F, de Freitas V. J Agric
Food Chem. 2011 May 25;59(10):5535-5547.
Protein identification using nano-HPLC-MS: ESI-MS and MALDI-MS interfaces. Vitorino R,
Krenkova J, Foret F, Domingues P, Amado F. Methods Mol Biol. 2011;790:31-46.
Salivary peptidomics. Amado F, Lobo MJ, Domingues P, Duarte JA, Vitorino R. Expert Rev
Proteomics. 2010 Oct;7(5):709-21.
Towards defining the whole salivary peptidome. Vitorino R, Barros A, Caseiro A,
Domingues P, Duarte J, Tomer K, Amado F. Proteomics Clin Applic. 2009; 3(5): 528 - 540.
GRANT SUPPORT
Principal Investigator
Ongoing projects
EXPL/BBB-BEP/0317/2012: “A selective magnetic nano-probe platform for enrichment of
proteases: MAGN@PROT”
Sponsors:
Prime-XS-000081: “Proteomic approach to identify and quantify cardiac mitochondrial protein
post-translational modifications-mediated by exercise training”
Sponsors:
Collaborator (Member of the Research Team)
Ongoing projects
PTDC/SAU-OSM/099762/2008: “Biochemical, morphological and functional evaluation of
breast cancer-induced muscle wasting: the role of exercise training”
PTDC/SAU-OSM/099762/2008: “In vitro alternative approach for chemical skin sensitization
hazard: an EU legislation demand.”
PTDC/SAU-OSM/101437/2008: “Development and application of P-glycoprotein inducers for
the prophylaxis and therapeutics of xenobiotics toxicity”.
PTDC/DES/103047/2008: “Influence of physical activity on bone tissue quality of female Wistar
rats with estrogen deficiency.”
PTDC/DES/104567/2008: “Mechanisms underlying cardiac protection induced by exercise in a
model of acute pressure overload.”
Sponsors:
Prime-XS-000222: “Modulation of estrogen receptor beta growth inhibitory effects by receptor
tyrosine kinases”
Sponsors:
Consultant
PTDC/SAU-ONC/112671/2009: “Effect of the microenvironment on post-translational
modifications of estrogen receptors.”
PTDC/SAU-ONC/118346/2010: “Epigenetic regulation in mammary stem/progenitor cells and
its contribution to malignant transformation.”
Sponsors: