52a Reunião Anual da Sociedade Brasileira de Zootecnia Zootecnia: Otimizando Recursos e Potencialidades Belo Horizonte – MG, 19 a 23 de Julho de 2015 Uso de câmera de infravermelho para predizer peso, composição corporal e de carcaça em bovinos Nelore e Angus¹ Gutierrez José de Freitas Assis2, Gustavo Reis Monteiro3, Marcio Machado Ladeira4, Rafael Aparecido Gomes3, Karina Costa Busato3, José Rodolfo Reis Carvalho3, Mario Luiz Chizzotti5. 1 Parte da dissertação de mestrado do segundo autor. Graduando em Zootecnia - UFV, Viçosa, Brasil. Bolsista de iniciação científica do CNPq, e-mail: [email protected] 3 Estudante de pós-graduação em Zootecnia - UFLA, Lavras, Brasil. 4 Professor Associado do Departamento de Zootecnia - UFLA, Lavras, Brasil. 5 Professor Adjunto do Departamento de Zootecnia - UFV, e-mail:[email protected] 2 Resumo: Objetivou-se com este trabalho, desenvolver e avaliar medidas biométricas obtidas por imagem de câmera de infravermelho. Foram utilizados 35 tourinhos, sendo 17 da raça Nelore e 18 da raça Angus. As medidas obtidas foram correlacionadas com variáveis relacionadas à composição corporal e de carcaça, assim como do peso corporal (Pc) dos animais. As imagens foram obtidas no dia anterior ao abate e, a partir delas, foram calculadas as seguintes medidas biométricas: altura (H), área do plano dorsal (APD), largura anterior (AW), Largura da costela (RW) e largura posterior (PW). A PW e H apresentaram correlação positiva com o peso corporal. A APD apresentou, nas duas raças, alta correlação com as variáveis de composição corporal e com o peso corporal (r = 0.90; 0.88, P<0.05). Foi desenvolvido um índice de composição corporal (BCI) a partir dos dados de Pc , H e APD. Em ambas as raças, o BCI estimou satisfatoriamente a porcentagem de extrato etéreo na carcaça e nos animais da raça Angus estimou, com maior precisão, os demais componentes corporais, em relação às estimativas baseadas apenas no Pc. O desenvolvimento de um índice de composição corporal a partir de medidas biométricas tem grande potencial para predição da composição da carcaça e ponto de abate. A APD mostrou-se eficiente na predição do peso corporal de bovinos. Palavras–chave: crescimento, carcaça, medidas biométricas, pecuária de precisão Using Infrared camera to predict body weight and composition of carcass and body in Nellore and Angus cattle¹ Abstract: The objective of this study was to develop and evaluate biometric measurements obtained by an infrared camera. There were used 35 young bulls, 17 of Nellore and 18 of Angus breed. Measurements obtained from images were correlated with variables related to body composition as well as with body weight (BW). The images were taken the day before slaughter and, from them, the following biometric measurements were calculated: height (H), dorsal plan area (DPA), anterior width (AW), rib width (RW) and posterior width (PW). The PW and H had a positive correlation with BW. DPA presented, in both breeds, a high correlation with the body composition variables as well as with BW(r = 0.90; 0.88, P<0.05). It was developed a body composition index (BCI) from measurements of BW, H and DPA. In both breeds, the BCI was able to estimate the percentage of ether extract in the carcass, and in Angus bulls, it estimated more accurately than BW the body composition. The development of a body composition index from biometric measurements has huge potential to predict carcass composition as well as slaughter point. The DPA was efficient in predicting cattle body weight. Keywords: biometric measurements, carcass, growth, precision livestock farming Introduction Body and carcass composition have great nutritional and economic importance in beef cattle production system. The estimation of body composition in live animals is crucial for the development of more efficient production systems that use less resource and increase the accuracy of nutrient requirements prediction and to support the decision of the optimal slaughter point to increase carcass quality (TEDESCHI; FOX; GUIROY, 2004). Infrared images are non invasive and a cost effective way to monitor animal development. Therefore it was aimed to evaluate and develop biometric measurements from infrared camera images that can predict body and carcass composition. Material e Methods Thirty five young bulls, being 17 of Nellore and 18 of Aberdeen Angus breed were used. In the day before slaughter, animals were conducted to a chute equipped with a infrared camera device adapted to collect images from above de dorsal plan of the animals (PreciZoo, Viçosa, Brazil). This apparatus is composed of an infrared laser projector and an infrared camera. A constant wave pattern is projected onto the subject and recorded by _____________________________________________________________________________________________________________________________ ___________________ Página - 1 - de 3 52a Reunião Anual da Sociedade Brasileira de Zootecnia Zootecnia: Otimizando Recursos e Potencialidades Belo Horizonte – MG, 19 a 23 de Julho de 2015 infrared camera. The distance between the camera and the subject (depth) is obtained by a triangulation process. This information is obtained by a standard splashed points created by the laser and captured by the infrared camera, forming a color gradient image. From the generated image, it was assessed the anterior, rib and posterior width of the animals, that were calculated by the difference of the pixel values points in x-axis, after calibration with a standard objected previously recorded under the same conditions. An image of the scale without the presence of the animal was also used to calibrate the sensor, and used to convert pixels of the images into mm. The measurement of the dorsal plan area (DPA) was determined from a perpendicular line just above animals shoulders. The software ImageJ (USA) was used to calculate the DPA from the collected infrared images, cleaned from pixels not related to animal’s body. For calibration of area measurement tool it was used the distance Arrow tool. The tolerance of the spectrum used in defining the selection area to be measured was 12 and the range for all measurements was 202.5 pixels per meter, obtained through the chute length. The day after the collection of images, all the animals were slaughtered. The gastrointestinal tract of each animal was emptied, washed, weighed and together with all other body components, was used to determine the empty body weight (EBW). Then, all non-carcass components were ground together and sampled. The carcass of each animal was divided into two half-carcasses, which were weighed and then cooled in a cold chamber at 2 ° C for 24 hours. The left half carcass, were dissected into separable muscle, bone and fat, that were weighed, and subsequently grounded, to represent a carcass sample. The chemical composition of carcass and empty body was then assessed accordinf to AOAC (1990) standard methods. Body crude protein (bCP) and ether extract (bEE) and carcass crude protein (bCP) and ether extract (cEE) were the determined. The Pearson’s correlations between biometric measurements and body and carcass variables were evaluated using PROC CORR of SAS 9.1 (Statistical Analysis System Institute - SAS Institute, 2003). Then, linear and stepwise regressions were applied to evaluate prediction equations using multiples biometric inputs, using PROC GLM of SAS 9.1 Results and Discussion In both breeds, the linear biometric measurements of AW, RW, PW and H showed no significant correlations with any chemical composition variable in body or carcass (p> 0.10). Similar results were reported by Ciryllo et al. (2003) who studied the relationship between hip height and length with carcass fat, kidney fat and visceral fat and found no significant correlation with them. Angus PW was correlated with BW, EBW and carcass weight (r = 0.64, 0.69 and 0.67, respectively). The height was only correlated (r = 0.47) with BW in Angus. In Nelore, PW was also correlated with BW, EBW and carcass weight (r = 0.51, 0.60 and 0.51). There was no correlation of height (p >0.05) with any variable in Nellore. DPA showed high correlation with physical and chemical body composition. DPA in Angus breed had significant correlation (p<0.05) with all characteristics studied, except for cCP and bCP, and was the best single measurement among those studied. Nonetheless, In Nellore, correlations of DPA with the proportions of ether extrat or protein in the body or carcass were not significant (P>0.05). From correlation of multiple biometric measurements with body composition, it was designed a body composition index: BCI = DPA0.75 × (1.86H × BW0.75)-1. The BCI was positively correlated with all body and carcass variables (P<0.05) in both breeds. BCI equations to predict fat content of carcass and body are presented in Table 1. Table 1. Predicting body and carcass ether extract content (bEE and cEE) from body composition index (BCI) in Nellore and Angus bulls. Equations n R2 p-value Angus bEE= -56.47 + 2.126*BCI 18 0.77 <0.0001 cEE = -24.18 + 1.213* BCI 18 0.72 <0.0001 Nellore bEE = 1.498 + 1.258 *BCI 17 0.61 0.0002 cEE = -7.773 + 1.100 *BCI 17 0.72 <0.0001 _____________________________________________________________________________________________________________________________ ___________________ Página - 2 - de 3 52a Reunião Anual da Sociedade Brasileira de Zootecnia Zootecnia: Otimizando Recursos e Potencialidades Belo Horizonte – MG, 19 a 23 de Julho de 2015 Additionally to estimate body composition, biometric measurements can be used to predict body weight, replacing traditional scales in beef production systems. The DPA presented high correlation with BW in both genetic groups studied. Therefore, equations to predict BW from images were developed (Table 2). Table 2. Body weight (BW, kg) predictions from dorsal plane area (DPA, cm2) in Angus and Nellore bulls Equation n R2 p-value Angus : BW = -195.02 +555,75* DPA 18 0.78 0.0017 Nellore: BW = 25.05 + 332,37 * DPA 17 0.80 0.0001 Conclusions Combined biometric measurements obtained from images were correlated with animal's body composition. The use of an index of body composition has potential to be used to estimate carcass composition. The dorsal plane area was efficient in predicting cattle body weight. Acknowledgements Authors thank the FAPEMIG and CNPq for having provided funds for this project. References Cyrillo, J.N.S.G. et al. Genetic changes to selection for yearling weight and correlated response on body measurements in Nelore cattle. In: WORLD CONGRESS ON GENETIC APPLIED TO LIVESTOCK PRODUCTION, 7. 2002, Montpellier. Proceedings... Montpellier: WCGALP, 2002. CD-ROM. TEDESCHI, L. O.; FOX, D. G.; GUIROY, P. J. A decision support system to improve individual cattle management: 1., a mechanistic, dynamic model for animal growth. Agricultural Systems, Essex, v. 79, n. 2, p. 171-204, Feb. 2004. STATISTICAL ANALYSIS SYSTEM INSTITUTE.User’s guide.Cary, 2003.129 p. _____________________________________________________________________________________________________________________________ ___________________ Página - 3 - de 3