4ta Reunión Annual PulseNet América Latina
Buenos Aires, Argentina
June , 2006
Experience
of
Instituto
Adolfo
Lutz,
Central
Public
Health
Laboratory, São Paulo State, Brazil , on the use of standardized
Pulsed-Field Gel Electrophoresis - PulseNet Protocol
Tânia Mara Ibelli Vaz
Instituto Adolfo Lutz
São Paulo, Brazil
SÃO PAULO STATE
BRASIL
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Instituto Adolfo Lutz *
Government of São Paulo State
Secretary of Health, São Paulo
Coordination of Diseases Control
CCD
Center of
Sanitary
Surveillance
Micro
Immunol
Leptospira
Dept.
Mycobacteria
Adolfo Lutz
Division of
Medical
Biology
Meningo, Pneumo,
Haemophilus,Enteroc
Instituto
Section of Bacteriology
Enterobacteria and
other Gram negative
Center of
Epidemiological
Surveillance
Instituto Adolfo Lutz
Central Public Health Laboratory
São Paulo State, Brazil
√ National Reference Laboratory for Escherichia coli (NRLEC)
Salmonella
√ Regional Reference Laboratory (RRL) Shigella
Vibrio cholerae
Strains to NRLEC / RRL sent by:
National Public Health Laboratories
NRLEC
Regional Laboratories of Instituto Adolfo Lutz (11 Laboratories)
Private and Public Hospital Laboratories
RRL
Central Public Health Laboratories
Paraná
Santa Catarina
Rio Grande do Sul
Mato Grosso do Sul
Characterization of Salmonella Enteritidis strains isolated from foodborne
outbreaks and from foods associated with these outbreaks
Fig.1A. XbaI PFGE patterns of S.Enteritidis
strains
1
2 3 4
5 6
7
8
9 10
1,5,10 , molecular marker, S. Braenderup H9812;
2,3,6,7,8 and 9 , S. Enteritidis strains from humans;
4, S. Enteritids from mayonnaise
Fig 1B. XbaI PFGE patterns of S.Enteritidis
strains
1
2 3 4 5 6
7 8 9
10
1,5,10 – molecular marker S. Braenderup H9812
4,6,7,8 and 9, S.Enteritidis strains from humans;
2 and 3, S.Enteritidis from food
Characterization of Salmonella Johannesburg strains isolated
from humans and foods
Fig. 2. XbaI PGFE patterns of S. Johannesburg strains from
humans and from foods
1
2
3
4
5
6
7
8
9
10
1,5,10 , molecular marker, Salmonella Braenderup H9812
2, 3 and 4, S. Johannesburg strains from humans;
6,7,8 and 9, S. Johannesburg strains from foods
Salmonella Typhi strains isolated from two typhoid fever (TF)
outbreaks and from sporadic cases of TF
Fig.3. XbaI PFGE patterns of SalmonellaTyphi
1
2
3
4
5
6
7
8
1,8 , molecular, marker, S.Braenderup H9812;
2,3, S.Typhi from sporadic cases of TF;
4,5 STyphi from outbreak A; 6,7, S.Typhi from outbreak B
Characterization of Salmonella I 1,4,[5],12:i:- and S.Typhimurium strains
isolated from human and non- human sources (1991-2000)
Fig 4A. XbaI PFGE patterns of
Salmonella I 1,4,[5],12:i:1
2
3 4
5 6 7 8 9 10 11 12 13 14 15
1,8,15 – Molecular marker, S. Braenderup
H9812 ( XbaI);2,3,4,5,6,7,9,10,11,12,13,14 ,
Salmonella I1,4,[5],12:i:-
Fig 4B. XbaI PFGE patterns of
SalmonellaTyphimurium
1
2 3 4 5
6 7 8
9 10 11 1 13 14 15
1,8,15 – molecular marker S. Braenderup H9812
2,3,4,5,6,7,9,10,11,12,13,14 – S. Typhimurium
Characterization of Salmonella Typhimurium isolated from sporadic
cases of human diseases and from non-human sources (2003-2004)
Fig.5 XbaI PFGE patterns of SalmonellaTyphimurium
1
2
3 4
5
6
7
8
9 10
11 12 13
1,7,13 – molecular marker, S. Braenderup H9812
2,3,4,5,6,8,9,10,11,12 – S. Typhimurium strains
Characterization of E.coli O157:H7
Fig 6.XbaI PFGE patterns of E.coli O157:H7
1
2
3
4
5
6
7
8
9
10
1,5 and10- molecular marker; 2,3,4,6,7,8 and 9 – E.coli o157:H7 strains
Tânia Mara Ibelli Vaz - VazTMI
Instituto Adolfo Lutz
Av.Dr.Arnaldo 351, 9o andar
CEP 01246-902
Cerqueira Cesar – São Paulo – SP
Brazil
[email protected]
[email protected]