CV Cláudio M. Gomes – Sept 2015
Curriculum vitae Cláudio M. Gomes
September 2015
Cláudio M. Gomes, PhD
Investigador Coordenador com agregação, Fellow of FCT investigator Programme
Group Leader of the FCUL Protein Folding and Misfolding Laboratory
Faculty of Sciences University of Lisboa (FCUL). Biosystems & Integrative Sciences Institute
Department Chemistry and Biochemistry. Bldg C8, Campo Grande 1749-016 Lisboa, Portugal
Tel: +351217500971. Skype: claudio.gomes
E-mail: [email protected] web page: folding.fc.ul.pt
Short Bio: Cláudio M. Gomes is Group Leader and a consolidation-level fellow (Investigador Coordenador) of the
FCT Investigator Programme at the Biosystems and Integrative Sciences Institute and Chemistry and Biochemistry
Department at the Faculty of Sciences University of Lisbon (FCUL) since 2015. From 2003-2014 he was group leader
and assistant researcher at the Instituto Tecnologia Química e Biologica at Universidade Nova de Lisboa. Born in
1970, graduated in Chemistry and Biotechnology at the Universidade Nova de Lisboa (1994), joined the Gulbenkian
Ph.D. program in Biology and Medicine (1994-1995), and got a Ph.D. degree in biochemistry at ITQB (1999). Until
setting up his independent laboratory (2004), he had a teaching position as Assistant Professor at the Faculdade de
Ciências e Tecnologia, Universidade Nova de Lisboa, (2000-2003). In 2013 he got the academic title of Agregado in
Biochemistry by the Universidade Nova de Lisboa, which is the highest academic title of the Portuguese Academic
system. His research interests focus on structural biology, biochemistry and protein biophysics (protein stability,
folding and aggregation). Since 2004, he has supervised over 20 research students (5 completed PhDs under sole
supervision) and is funded by national and international grants. He has served as expert project reviewer for the
European Commission and over 10 international foundations and funding agencies in Europe and in the US, is a
member of the Editorial board or Guest Editor in 5 scientific journals, and has regular activity as peer-reviewer in
over >30 international journals. Editor of the SpringerBriefs Series on ‘Protein Folding and Structure’ (2013) has
also edited the book ‘Protein Folding and Metal Ions: Mechanisms, Biology and Disease’ with CRC Press (2010). Dr.
Gomes has published 97 peer-reviewed papers in ISI journals, 1 edited book, 1 authored book (forthcoming in
2015) and 5 chapters in books, with over 1800 citations (non self). His current h-index is 27 (Web of Science).
Current Position
2015- Fellow of FCT investigator Programme (Investigador Coordenador) at FCUL
Biosystems & Integrative Sciences Institute and Group leader of the Protein Folding & Misfolding Lab
Education
1994 Degree in Applied Chemistry/Biotechnology (FCT/UNL, Monte de Caparica)
1995 Gulbenkian Ph.D. Program in Biology and Medicine (IGC, Oeiras)
1999 Ph.D. in Biochemistry, Universidade Nova de Lisboa (ITQB/UNL)
2013 Habilitation (Agregação) in Biochemistry, Universidade Nova de Lisboa (UNL)
Honors and fellowships
2011 Terry Fox Award for Oncology Research (Portuguese Cancer League)
2000 European Molecular Biology Organisation (EMBO) short term fellowship
2000 Research Award Calouste Gulbenkian Foundation for Research stimuli
1994 PhD Fellowship, Portuguese Ministry of Science and Technology
Academic appointments
2003-2014
Assistant Researcher, Independent Group leader at ITQB New University of Lisbon (Oeiras)
2000-2003
Assistant Professor (FCT New University of Lisbon, Caparica)
2000-2001
Assistant Professor (Portuguese Catholic University, Sintra)
1999-2003
Researcher (post-doctoral) (ITQB/UNL, Oeiras)
1995-1998
Ph.D. student. Topic: Bioenergetics (ITQB/UNL, Oeiras)
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International Research Training
2002 University of Rome ‘La Sapienza’, Rome University, Italy (A. Giuffrè)
2002 Biocentrum Helsinkii, University of Helsinki, Finland (M. Wikström)
1998 Göteborg University, Sweden (P. Brzezinski)
1996 University of Regensburg, Germany (K. O. Stetter)
1996 University of Rome ‘La Sapienza’, Italy (M. Brunori)
Languages
English (proficiency Certificate from Cambridge University); French (good); Portuguese (mother tongue)
Professional Societies
International: Protein Society  Biochemical Society (UK)  Society for Biological Inorganic Chemistry
National: Chemistry Society  Biochemical Society  Biophysical Society.
Financed research projects (see annex for funding breakdown analysis)
 Fundação Ciência e Tecnologia FCT/MEES (Portuguese Research Agency 2003-2014: 7 projects as PI)
2012-2014 Mechanisms of SOD1 toxic aggregation in neurodegenerative processes
2012-2014 Recovery of aggregated proteins using biological nanoreactors and small molecules
2007-2010 Functional analysis of MADD pathophysiology in fatty acid oxidation pathways
2007-2010 Energetic and structural interplay between metal sites and protein folding
2005-2009 Screening hyperthermophilic Proteomes for Hyperstable Proteins
2002-2005 Understanding the intrinsic stability mechanisms of hyperthermophilic metalloproteins
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CLIMB (UK) Research Grant - National Information Centre for Metabolic Diseases (2011-2014)
Terry Fox Research Grant – Liga Portuguesa contra o Cancro (2011-2013)
National Ataxia Foundation (USA) Research Grant (2008-2009)
Several Bilateral exchange cooperation grants (Portugal and. – Italy, –Germany, –UK,)
Scientific Supervision and Research Group
Independent Scientific supervision since 2004 (completed): 5 PhDs; 4 Post-Docs; 6 MSc; 2 Erasmus students Current composition and past members: here
Experience as expert reviewer (research grants evaluation)
 European Commission Horizon2020 (2014-2015)
 Agence Nationale de Recherche, France (2013, 2014, 2015)
 Telethon Foundation, Italy (2014)
 Industrial Research Fund, University of Leuven, Belgium (2013)
 The Royal Swedish Academy of Sciences, Sweden (Wallenberg Fellows, 2012, 2013)
 Instituto Pasteur-Fondazione Cenci Bolognetti, Italy (2013)
 Israel Science Foundation, Israel (2013)
 Research Foundation Flanders, Belgium (2012)
 Romanian National Research Council, Romania (2012)
 Prinses Beatrix Fonds, Amsterdam, The Netherlands (2010, 2014)
 Fondazione Cariplo Milano, Italy (Advisory Board), (2010)
 Smithsonian Institution, Washington D.C., USA (2008)
Service as referee for International Journals
Reviewer in >30 international journals, including: Nature Protocols  Coordination Chemistry Reviews  Chemistry
and Biology  Journal Molecular Biology  Proteins  Biochemistry  Journal Biological Inorganic Chemistry  FEBS
Letters  International J Biological Macromolecules  IUMB Life  International Journal of Molecular Sciences 
Journal of Proteome Research  Protein Engineering design & Selection  Human Mutation  PLoS One
Editorial activity in international scientific journals
2015- Associate Editor, Biochemistry and Biophysics Reports (Elsevier)
2013- Editorial Board, Scientific Reports (Nature Publishing Group)
2013- Editorial Board, Current Topics in Medicinal Chemistry (Benthams)
2012- Academic Editor, PLOS ONE (PLOS)
2011- Editorial Board, Frontiers in Fatty Acid and Lipid Physiology
12-13 Editorial Board, Dataset Papers in Biology - Biochemistry section
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10-14 Editorial Board, Bioinorganic Chemistry and Applications
Editorial activity with Publishing Houses
2013- Series Editor SpringerBriefs on ‘Protein Structure and Folding’, Springer, Germany
2015 Book author ‘Protein Folding - An Introduction’ By Gomes, C.M. and Faisca, P.F.N. forthcoming title
2010 Editor of the Book ‘Protein Folding and Metal Ions: Mechanisms, Biology and Disease’ CRC Press, USA
Oral Presentations as invited speaker in International meetings
 6th EuChem in Life Sciences, Lisboa, Portugal (2015)
 EMBO meeting Molecular chaperones: From molecules to cells and misfolding diseases, Heraklion, Greece (2015)
 PROTSTAB14 Protein Stability congress, Stresa, Italy (2014)
 9th European Biophysics Congress, Lisbon, Portugal (2013)
 2nd Iberian Congress on Prions, Faro, Portugal (2013)
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 36 FEBS Congress, Torino, Italy (2011)
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IV Spanish Portuguese Biophysical Congress, Zaragoza, Spain (2010)
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EMBO-FEBS Conference Biology Molecular Chaperones, Dubronik, Croatia (2009)
VIII Meeting International Consortium on SOD and ALS research, ICOSA8 (2008)
EMBO-FEBS Workshop on Chaperones in Normal & Aberrant Protein Folding, Aging & Cancer (2007)
8th European Bioinorganic Chemistry Conference (2006)
VI Ibero-American Congress of Biophysics (2006)
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Invited Research Seminars in international academic Departments
 Center for Alzheimer Research at Karolinska Institutet, Sweden (2015)
 Institute Biotechnology and Biomedicine, Universidad Autononoma Barcelona, Spain (2014)
 Department of Cellular and Molecular Medicine, Catholic University Leuven, Belgium (2013)
 Université Paul Sabatier, Toulouse, France (2011)
 Chemistry Department, University of Torino (2011)
 Institute of Biomedicine, CSIC Valencia, Spain (2011)
 Institute Européene Chimie et Biologie, Bordeaux, France (2011)
 Academic Medical Center, University of Amsterdam (2010)
 Aarhus University, Denmark (2010)
 Medical Biochemistry and Biophysics Department, University Umea, Sweden (March 2009)
Session Chair in International Meetings
 Chair ‘Intrinsically Disordered Proteins’ Session – EBSA Meeting, Lisboa (2013)
 Chair ‘Protein Folding’ Session, IV Spanish Portuguese Biophysical Congress, Zaragoza (2010)
 Chair ‘Metalloproteins in Health’ Session, 8th European Bioinorganic Chemistry Conference (2006)
Organisation of Scientific meetings
2011 Symposium ‘Frontiers in Protein Research'. ITQB (chair)
2010 Frontiers in Chemical Biology. ITQB.
2009 Protein Folding Diseases: from metabolic disorders to neurodegeneration. ITQB (Chair)
2002 Bioenergetics: the Cellular Power Plants Santarém
2001 FEBS Forum of Young Scientists: Protein structure-function, trafficking and signalling. ITQB
Teaching
 >1200h (contact time) of undergraduate teaching (theoretical and practical courses) from 2000 to 2003
 Undergraduate teaching: Biochemistry (2000-2003) and Food Biochemistry (2002) courses at FCT/UNL
 Invited lectures: Fac Ciencias Medicas – Protein Folding lecture to MD students (2008-present)
 Post-Graduate: ITQB and Gulbenkian Biomedicine PhD Program - ‘Protein Folding’ Lectures (2007-)
 International: Graduate PhD Program in Translational Medicine, Aarhus University (2010-present)
 Invited lectures in several national MSc Courses
 Coordinator of ITQB/New University of Lisbon Research Training Courses (2010-2014)
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PhD thesis examination juries
 Participation in 13 PhD juries as main opponent and in 7 as voting member.
 External Supervisor of S. Hagmeyer (Ulm University, Germany; Supervisor: A. Grabruckner).
 Universidade de Coimbra (2014), Universidade de Aveiro (2013), Universidade de Lisboa (2013, 2010, 2008)
 Université Toulouse Paul Sabatier (2012), University of Rome ‘La Sapienza’ (2007)
Science Dissemination activities to the public and media
 Public conferences: European Researcher’s Night (2008; 2012), ITQB Open Day (2009)
 Outreach activities to students: University students ‘Investiga ITQB’ (2010); high school visits (2/year)
 Media appearances on TV: Telejornal RTP1 (2008); Com Ciência RTP2 (2012); Radio: Prova Oral (2008).
 News Highlights: A new model for a rare disease (2012), Protein Folding Metal ions (2010), Trading off stability for
activity (2010), Vit B2 and metabolism (2009), Prizes at EUROBIC 8 (2006)
Management Experience in International projects
 EU- Project INTERBIO (EU-Sudoe) Project 2009-2012: Lisbon-node Coordinator (2011), Scientific Committee
Member and coordinator for the WP4 ‘Technological platforms’. Partners: Barcelona (BIOCAT), Bordeaux
(IECB), Lisbon (ITQB), Toulouse (UPS) and Valencia (FIVEC).
 Zinc-Net ‘The Biology of Zinc’ – COST action TD1304, Core Group Member, Management Committee and STST
committee member. National delegate.
Research
Major research theme focus on protein misfolding in human disease, specifically protein aggregation mechanisms
in neurodegeneration (Alzheimer’s disease, amyotrophic lateral sclerosis) and human metabolic diseases
(mitochondrial disorders). Our research is aimed at understanding the biology and biophysics of protein folding, an
essential cellular process through which proteins acquire a functional conformation. We are interested in
establishing which mechanisms trigger protein misfolding, in identifying determinants of protein stability, and in
addressing how protein interactions, metal ions, small molecules and post translational modifications influence the
protein folding process. We are also interested in molecular chaperones and pharmacological recovery of protein
folding defects by small molecules (pharmacological chaperones).
Research Keywords
Protein Folding, Amyloid, Metal ions, Structural Biology, Proteomics, Oxidative stress, Neurodegeneration, Inborn
errors of metabolism, pharmacological protein stabilisers, biophysical spectroscopy
International collaborative network (2004-present)
Antonio Pineda-Lucena (CIPF, Spain)  Andreas Grabrucker (Ulm Univ, Germany)  Antje Biesemeier (Tuebingen U,
DE)  Annalisa Pastore (NIMR, UK)  Elizabeth E. Craig (Wisconsin Univ, USA)  Fred Rousseau/Joost Schymkowitz
(Katholic Univ Leuven, Belgium)  Guenter Fritz (Freiburg Univ, Germany)  Joan S. Valentine (UCLA, USA)  Ludmilla
Morozova-Roche (Umea Univ, Sweden)  Mark Fisher (Kansas U Med Center, USA)  Matthew Hirschey (Duke U
Med Center, USA)  Mikael Oliveberg (Stockholm Univ, Sweden)  Peter Bross (Aarhus Univ, Denmark)  Peter Faller
(Toulouse Univ, France)  Phillip Wright (Sheffield Univ, UK)  Sima Lev (Weizmann Inst, Israel)  Vesna Vesna
Dragutinovic (Belgrade Univ, Serbia)  Wojciech Bal (Warsaw Univ, Poland)
Summary of Scientific outputs
97 papers in international peer reviewed journals (ISI web of Knowledge papers)
5 book chapters  24 papers published as proceedings
Complete and updated list of papers: http://folding.fc.ul.pt/?page_id=32 or PubMed
Other publication lists (and metrics) at: Google Scholar
Web of Science Metrics: Sum of the Times Cited (non self): >1800; h-index: 27.
Google scholar metrics: Citations: >2500; h-index: 29
ORCID: http://orcid.org/0000-0003-4662-6933
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CV Cláudio M. Gomes – Sept 2015
Full list of publications 1995-2014
International peer reviewed journals indexed in web of Science.
For updated number of citations per paper and journal links, please refer to my google scholar profile.
Latest paper
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Leal, S.S., Gomes, C.M.(2015) Calcium dysregulation links ALS defective proteins and motor neuron
selective vulnerability, Frontiers in Cellular Neuroscience (2015) doi:10.3389/ fncel.2015.0022529
Leal, S. S., Cristovao, J. S., Biesemeier, A., Cardoso, I., and Gomes, C. M. (2014) Aberrant zinc binding to
immature conformers of metal-free copper-zinc superoxide dismutase triggers amorphous aggregation,
Metallomics 7(2):333-46
Henriques, B. J., Lucas, T. G., Rodrigues, J. V., Frederiksen, J. H., Teixeira, M. S., Tiranti, V., Bross, P., and
Gomes, C. M. (2014) Ethylmalonic Encephalopathy ETHE1 R163W/R163Q Mutations Alter Protein Stability
and Redox Properties of the Iron Centre, PloS one 9, e107157.
Estacio, S. G., Leal, S. S., Cristovao, J. S., Faisca, P. F., and Gomes, C. M. (2014) Calcium binding to
gatekeeper residues flanking aggregation-prone segments underlies non-fibrillar amyloid traits in
superoxide dismutase 1 (SOD1), Biochim Biophys Acta 1854, 118-126.
Deive, F. J., Ruivo, D., Rodrigues, J. V., Gomes, C. M., Sanroman, M. A., Rebelo, L. P. N., Esperanca, J. M. S.
S., and Rodriguez, A. (2014) On the hunt for truly biocompatible ionic liquids for lipase-catalyzed reactions,
RSC Advances 5, 3386-3389.
Rodrigues, J. V., Ruivo, D., Rodriguez, A., Deive, F. J., Esperanca, J. M. S. S., Marrucho, I. M., Gomes, C. M.,
and Rebelo, L. P. N. (2014) Structural-functional evaluation of ionic liquid libraries for the design of cosolvents in lipase-catalysed reactions, Green Chemistry 16 (10), 4520-4523
Corte, L., Valente, F., Serrano, M., Gomes, C. M., Moran, C. P., Jr., and Henriques, A. O. (2014) A Conserved
Cysteine Residue of Bacillus subtilis SpoIIIJ Is Important for Endospore Development, PloS one 9, e99811.
Correia, A. R., Naik, S., Fisher, M. T., and Gomes, C. M. (2014) Probing the kinetic stabilities of Friedreich's
ataxia clinical variants using a solid phase GroEL chaperonin capture platform, Biomolecules 4, 956-979.
Leal, S. S., Cardoso, I., Valentine, J. S., and Gomes, C. M. (2013) Calcium Ions Promote Superoxide
Dismutase 1 (SOD1) Aggregation into Non-fibrillar Amyloid: a link to toxic effects of calcium overload in
amyotrophic lateral sclerosis (ALS)?, J Biol Chem 288, 25219-25228.
Gomes, C. M., and Santos, R. (2013) Neurodegeneration in Friedreich's Ataxia: From Defective Frataxin to
Oxidative Stress, Oxid Med Cell Longev 2013, 487534.
Farinha, C. M., King-Underwood, J., Sousa, M., Correia, A. R., Henriques, B. J., Roxo-Rosa, M., Da Paula, A.
C., Williams, J., Hirst, S., Gomes, C. M., and Amaral, M. D. (2013) Revertants, Low Temperature, and
Correctors Reveal the Mechanism of F508del-CFTR Rescue by VX-809 and Suggest Multiple Agents for Full
Correction, Chemistry & biology 20, 943-955.
Cristovao, J. S., Leal, S. S., Cardoso, I., and Gomes, C. M. (2013) Small molecules present in the
cerebrospinal fluid metabolome influence superoxide dismutase 1 aggregation, Int J Mol Sci 14, 1912819145.
Carvalho, S. B., Botelho, H. M., Leal, S. S., Cardoso, I., Fritz, G., and Gomes, C. M. (2013) Intrinsically
Disordered and Aggregation Prone Regions Underlie beta-Aggregation in S100 Proteins, PloS one 8,
e76629.
Veith, A., Botelho, H. M., Kindinger, F., Gomes, C. M., and Kletzin, A. (2012) The sulfur oxygenase reductase
from the mesophilic bacterium Halothiobacillus neapolitanus is a highly active thermozyme, Journal of
bacteriology 194, 677-685.
Silva, M. A., Lucas, T. G., Salgueiro, C. A., and Gomes, C. M. (2012) Protein folding modulates the swapped
dimerization mechanism of methyl-accepting chemotaxis heme sensors, PloS one 7, e46328.
Rodrigues, J. V., Henriques, B. J., Lucas, T. G., and Gomes, C. M. (2012) Cofactors and metabolites as
protein folding helpers in metabolic diseases, Curr Top Med Chem 12, 2546-2559.
Rodrigues, J. V., and Gomes, C. M. (2012) Mechanism of superoxide and hydrogen peroxide generation by
human electron-transfer flavoprotein and pathological variants, Free radical biology & medicine 53, 12-19.
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Leal, S. S., Botelho, H. M., and Gomes, C. M. (2012) Metal ions as modulators of protein conformation and
misfolding in neurodegeneration, Coordination Chemistry Reviews 256, 2253-2270.
Gomes, C. M. (2012) Protein misfolding in disease and small molecule therapies, Curr Top Med Chem 12,
2460-2469.
Botelho, H. M., Leal, S. S., Cardoso, I., Yanamandra, K., Morozova-Roche, L. A., Fritz, G., and Gomes, C. M.
(2012) S100A6 Amyloid Fibril Formation Is Calcium-modulated and Enhances Superoxide Dismutase-1
(SOD1) Aggregation, J Biol Chem 287, 42233-42242.
Alves, E., Henriques, B. J., Rodrigues, J. V., Prudencio, P., Rocha, H., Vilarinho, L., Martinho, R. G., and
Gomes, C. M. (2012) Mutations at the flavin binding site of ETF:QO yield a MADD-like severe phenotype in
Drosophila, Biochim Biophys Acta 1822, 1284-1292.
Rodrigues, J. V., Prosinecki, V., Marrucho, I., Rebelo, L. P., and Gomes, C. M. (2011) Protein stability in an
ionic liquid milieu: on the use of differential scanning fluorimetry, Physical chemistry chemical physics :
PCCP 13, 13614-13616.
Lucas, T. G., Henriques, B. J., Rodrigues, J. V., Bross, P., Gregersen, N., and Gomes, C. M. (2011) Cofactors
and metabolites as potential stabilizers of mitochondrial acyl-CoA dehydrogenases, Biochim Biophys Acta
1812, 1658-1663.
Henriques, B. J., Fisher, M. T., Bross, P., and Gomes, C. M. (2011) A polymorphic position in electron
transfer flavoprotein modulates kinetic stability as evidenced by thermal stress, FEBS Lett 585, 505-510.
Botelho, H. M., and Gomes, C. M. (2011) Structural reorganization renders enhanced metalloprotein
stability, Chem Commun (Camb) 47, 11149-11151.
Rodrigues, J. V., and Gomes, C. M. (2010) Enhanced superoxide and hydrogen peroxide detection in
biological assays, Free radical biology & medicine 49, 61-66.
Kim, S., Leal, S. S., Ben Halevy, D., Gomes, C. M., and Lev, S. (2010) Structural requirements for VAP-B
oligomerization and their implication in amyotrophic lateral sclerosis-associated VAP-B(P56S) neurotoxicity,
J Biol Chem 285, 13839-13849.
Henriques, B. J., Olsen, R. K., Bross, P., and Gomes, C. M. (2010) Emerging roles for riboflavin in functional
rescue of mitochondrial beta-oxidation flavoenzymes, Current medicinal chemistry 17, 3842-3854.
Henriques, B. J., Bross, P., and Gomes, C. M. (2010) Mutational hotspots in electron transfer flavoprotein
underlie defective folding and function in multiple acyl-CoA dehydrogenase deficiency, Biochim Biophys
Acta 1802, 1070-1077.
Fritz, G., Botelho, H. M., Morozova-Roche, L. A., and Gomes, C. M. (2010) Natural and amyloid selfassembly of S100 proteins: structural basis of functional diversity, The FEBS journal 277, 4578-4590.
Correia, A. R., Wang, T., Craig, E. A., and Gomes, C. M. (2010) Iron-binding activity in yeast frataxin entails a
trade off with stability in the alpha1/beta1 acidic ridge region, Biochem J 426, 197-203.
Botelho, H. M., Leal, S. S., Veith, A., Prosinecki, V., Bauer, C., Frohlich, R., Kletzin, A., and Gomes, C. M.
(2010) Role of a novel disulfide bridge within the all-beta fold of soluble Rieske proteins, J Biol Inorg Chem
15, 271-281.
Amaral, J. D., Correia, A. R., Steer, C. J., Gomes, C. M., and Rodrigues, C. M. (2010) No evidence of direct
binding between ursodeoxycholic acid and the p53 DNA-binding domain, Bioscience reports 30, 359-364.
Henriques, B. J., Rodrigues, J. V., Olsen, R. K., Bross, P., and Gomes, C. M. (2009) Role of flavinylation in a
mild variant of multiple acyl-CoA dehydrogenation deficiency: a molecular rationale for the effects of
riboflavin supplementation, J Biol Chem 284, 4222-4229.
Correia, A. R., Ow, S. Y., Wright, P. C., and Gomes, C. M. (2009) The conserved Trp155 in human frataxin as
a hotspot for oxidative stress related chemical modifications, Biochem Biophys Res Commun 390, 10071011.
Botelho, H. M., Koch, M., Fritz, G., and Gomes, C. M. (2009) Metal ions modulate the folding and stability of
the tumor suppressor protein S100A2, The FEBS journal 276, 1776-1786.
Boscolo, B., Leal, S. S., Salgueiro, C. A., Ghibaudi, E. M., and Gomes, C. M. (2009) The prominent
conformational plasticity of lactoperoxidase: a chemical and pH stability analysis, Biochim Biophys Acta
1794, 1041-1048.
Stelter, M., Melo, A. M., Pereira, M. M., Gomes, C. M., Hreggvidsson, G. O., Hjorleifsdottir, S., Saraiva, L. M.,
Teixeira, M., and Archer, M. (2008) A novel type of monoheme cytochrome c: biochemical and structural
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characterization at 1.23 A resolution of rhodothermus marinus cytochrome c, Biochemistry 47, 1195311963.
Leandro, P., and Gomes, C. M. (2008) Protein misfolding in conformational disorders: rescue of folding
defects and chemical chaperoning, Mini Rev Med Chem 8, 901-911.
Leal, S. S., and Gomes, C. M. (2008) On the relative contribution of ionic interactions over iron-sulfur
clusters to ferredoxin stability, Biochim Biophys Acta 1784, 1596-1600.
Gouveia, R. M., Gomes, C. M., Sousa, M., Alves, P. M., and Costa, J. (2008) Kinetic analysis of L1 homophilic
interaction: role of the first four immunoglobulin domains and implications on binding mechanism, J Biol
Chem 283, 28038-28047.
Frazao, C., Aragao, D., Coelho, R., Leal, S. S., Gomes, C. M., Teixeira, M., and Carrondo, M. A. (2008)
Crystallographic analysis of the intact metal centres [3Fe-4S](1+/0) and [4Fe-4S](2+/1+) in a Zn(2+)containing ferredoxin, FEBS Lett 582, 763-767.
Faisca, P. F., and Gomes, C. M. (2008) On the relation between native geometry and conformational
plasticity, Biophys Chem 138, 99-106.
Correia, A. R., Pastore, C., Adinolfi, S., Pastore, A., and Gomes, C. M. (2008) Dynamics, stability and ironbinding activity of frataxin clinical mutants, The FEBS journal 275, 3680-3690.
Todorovic, S., Leal, S. S., Salgueiro, C. A., Zebger, I., Hildebrandt, P., Murgida, D. H., and Gomes, C. M.
(2007) A Spectroscopic Study of the Temperature Induced Modifications on Ferredoxin Folding and IronSulfur Moieties, Biochemistry 46, 10733-10738.
Prosinecki, V., Faísca, P. F. N., and Gomes, C. M. (2007) Conformational states and protein stability in a
proteomic perspective., Current Proteomics 4, 44-52.
Liz, M. A., Gomes, C. M., Saraiva, M. J., and Sousa, M. M. (2007) ApoA-I cleaved by transthyretin has
reduced ability to promote cholesterol efflux and increased amyloidogenicity, J Lipid Res 48, 2385-2395.
Leal, S. S., and Gomes, C. M. (2007) Studies of the molten globule state of ferredoxin: Structural
characterization and implications on protein folding and iron-sulfur center assembly, Proteins 68, 606-616.
Boscolo, B., Leal, S. S., Ghibaudi, E. M., and Gomes, C. M. (2007) Lactoperoxidase folding and catalysis relies
on the stabilization of the alpha-helix rich core domain: A thermal unfolding study, Biochim Biophys Acta
1774, 1164-1172.
Urich, T., Gomes, C. M., Kletzin, A., and Frazao, C. (2006) X-ray Structure of a self-compartmentalizing
sulfur cycle metalloenzyme, Science 311, 996-1000.
Rocha, R., Leal, S. S., Teixeira, V. H., Regalla, M., Huber, H., Baptista, A. M., Soares, C. M., and Gomes, C. M.
(2006) Natural domain design: enhanced thermal stability of a zinc-lacking ferredoxin isoform shows that a
hydrophobic core efficiently replaces the structural metal site, Biochemistry 45, 10376-10384.
Prosinecki, V., Botelho, H. M., Francese, S., Mastrobuoni, G., Moneti, G., Urich, T., Kletzin, A., and Gomes,
C. M. (2006) A Proteomic Approach toward the Selection of Proteins with Enhanced Intrinsic
Conformational Stability, J Proteome Res 5, 2720-2726.
Henriques, B. J., Saraiva, L. M., and Gomes, C. M. (2006) Combined spectroscopic and calorimetric
characterisation of rubredoxin reversible thermal transition, J Biol Inorg Chem 11, 73-81.
Correia, A. R., Adinolfi, S., Pastore, A., and Gomes, C. M. (2006) Conformational stability of human frataxin
and effect of Friedreich's ataxia-related mutations on protein folding, Biochem J 398, 605-611.
Leal, S. S., and Gomes, C. M. (2005) Linear three-iron centres are unlikely cluster degradation intermediates
during unfolding of iron-sulfur proteins, Biol Chem 386, 1295-1300.
Kletzin, A., Ferreira, A. S., Hechler, T., Bandeiras, T. M., Teixeira, M., and Gomes, C. M. (2005) A Rieske
ferredoxin typifying a subtype within Rieske proteins: spectroscopic, biochemical and stability studies, FEBS
Lett 579, 1020-1026.
Henriques, B. J., Saraiva, L. M., and Gomes, C. M. (2005) Probing the mechanism of rubredoxin thermal
unfolding in the absence of salt bridges by temperature jump experiments, Biochem Biophys Res Commun
333, 839-844.
Urich, T., Bandeiras, T. M., Leal, S. S., Rachel, R., Albrecht, T., Zimmermann, P., Scholz, C., Teixeira, M.,
Gomes, C. M., and Kletzin, A. (2004) The sulphur oxygenase reductase from Acidianus ambivalens is a
multimeric protein containing a low-potential mononuclear non-haem iron centre, Biochem J 381, 137146.
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Muller, F. H., Bandeiras, T. M., Urich, T., Teixeira, M., Gomes, C. M., and Kletzin, A. (2004) Coupling of the
pathway of sulphur oxidation to dioxygen reduction: characterization of a novel membrane-bound
thiosulphate:quinone oxidoreductase, Mol Microbiol 53, 1147-1160.
Leal, S. S., Teixeira, M., and Gomes, C. M. (2004) Studies on the degradation pathway of iron-sulfur centers
during unfolding of a hyperstable ferredoxin: cluster dissociation, iron release and protein stability, J Biol
Inorg Chem 9, 987-996.
Kletzin, A., Urich, T., Muller, F., Bandeiras, T. M., and Gomes, C. M. (2004) Dissimilatory oxidation and
reduction of elemental sulfur in thermophilic archaea, J Bioenerg Biomembr 36, 77-91.
Das, T. K., Gomes, C. M., Bandeiras, T. M., Pereira, M. M., Teixeira, M., and Rousseau, D. L. (2004) Active
site structure of the aa3 quinol oxidase of Acidianus ambivalens, Biochim Biophys Acta 1655, 306-320.
Victor, B. L., Vicente, J. B., Rodrigues, R., Oliveira, S., Rodrigues-Pousada, C., Frazao, C., Gomes, C. M.,
Teixeira, M., and Soares, C. M. (2003) Docking and electron transfer studies between rubredoxin and
rubredoxin:oxygen oxidoreductase, J Biol Inorg Chem 8, 475-488.
Hellwig, P., Gomes, C. M., and Teixeira, M. (2003) FTIR spectroscopic characterization of the cytochrome
aa3 from Acidianus ambivalens: evidence for the involvement of acidic residues in redox coupled proton
translocation, Biochemistry 42, 6179-6184.
Bandeiras, T. M., Salgueiro, C. A., Huber, H., Gomes, C. M., and Teixeira, M. (2003) The respiratory chain of
the thermophilic archaeon Sulfolobus metallicus: studies on the type-II NADH dehydrogenase, Biochim
Biophys Acta 1557, 13-19.
Vicente, J. B., Gomes, C. M., Wasserfallen, A., and Teixeira, M. (2002) Module fusion in an A-type
flavoprotein from the cyanobacterium Synechocystis condenses a multiple-component pathway in a single
polypeptide chain, Biochem Biophys Res Commun 294, 82-87.
Pereira, M. M., Gomes, C. M., and Teixeira, M. (2002) Plasticity of proton pathways in haem-copper oxygen
reductases, FEBS Lett 522, 14-18.
Lemos, R. S., Gomes, C. M., LeGall, J., Xavier, A. V., and Teixeira, M. (2002) The quinol:fumarate
oxidoreductase from the sulphate reducing bacterium Desulfovibrio gigas: spectroscopic and redox studies,
J Bioenerg Biomembr 34, 21-30.
Lemos, R. S., Fernandes, A. S., Pereira, M. M., Gomes, C. M., and Teixeira, M. (2002) Quinol:fumarate
oxidoreductases and succinate:quinone oxidoreductases: phylogenetic relationships, metal centres and
membrane attachment, Biochim Biophys Acta 1553, 158-170.
Jones, K., Gomes, C. M., Huber, H., Teixeira, M., and Wittung-Stafshede, P. (2002) Formation of a linear
[3Fe-4S] cluster in a seven-iron ferredoxin triggered by polypeptide unfolding, J Biol Inorg Chem 7, 357-362.
Gomes, C. M., Kletzin, A., and Teixeira, M. (2002) An archaeal b-type cytochrome containing a
nonfunctional carbonic anhydrase-like domain, J Biol Inorg Chem 7, 483-489.
Gomes, C. M., Giuffre, A., Forte, E., Vicente, J. B., Saraiva, L. M., Brunori, M., and Teixeira, M. (2002) A novel
type of nitric-oxide reductase. Escherichia coli flavorubredoxin, J Biol Chem 277, 25273-25276.
Gomes, C. M., Frazao, C., Xavier, A. V., Legall, J., and Teixeira, M. (2002) Functional control of the binuclear
metal site in the metallo-beta-lactamase-like fold by subtle amino acid replacements, Protein Sci 11, 707712.
Bandeiras, T. M., Salgueiro, C., Kletzin, A., Gomes, C. M., and Teixeira, M. (2002) Acidianus ambivalens
type-II NADH dehydrogenase: genetic characterisation and identification of the flavin moiety as FMN, FEBS
Lett 531, 273-277.
Moczygemba, C., Guidry, J., Jones, K. L., Gomes, C. M., Teixeira, M., and Wittung-Stafshede, P. (2001) High
stability of a ferredoxin from the hyperthermophilic archaeon A. ambivalens: involvement of electrostatic
interactions and cofactors, Protein Sci 10, 1539-1548.
Lemos, R. S., Gomes, C. M., and Teixeira, M. (2001) Acidianus ambivalens Complex II typifies a novel family
of succinate dehydrogenases, Biochem Biophys Res Commun 281, 141-150.
Lemos, R. S., Gomes, C. M., Santana, M., LeGall, J., Xavier, A. V., and Teixeira, M. (2001) The 'strict'
anaerobe Desulfovibrio gigas contains a membrane-bound oxygen-reducing respiratory chain, FEBS Lett
496, 40-43.
Gomes, C. M., Le Gall, J., Xavier, A. V., and Teixeira, M. (2001) Could a diiron-containing four-helix-bundle
protein have been a primitive oxygen reductase?, Chembiochem 2, 583-587.
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Gomes, C. M., Bandeiras, T. M., and Teixeira, M. (2001) A new type-II NADH dehydrogenase from the
archaeon Acidianus ambivalens: characterization and in vitro reconstitution of the respiratory chain, J
Bioenerg Biomembr 33, 1-8.
Gomes, C. M., Backgren, C., Teixeira, M., Puustinen, A., Verkhovskaya, M. L., Wikstrom, M., and
Verkhovsky, M. I. (2001) Heme-copper oxidases with modified D- and K-pathways are yet efficient proton
pumps, FEBS Lett 497, 159-164.
Gilderson, G., Aagaard, A., Gomes, C. M., Adelroth, P., Teixeira, M., and Brzezinski, P. (2001) Kinetics of
electron and proton transfer during O(2) reduction in cytochrome aa(3) from A. ambivalens: an enzyme
lacking Glu(I-286), Biochim Biophys Acta 1503, 261-270.
Wittung-Stafshede, P., Gomes, C. M., and Teixeira, M. (2000) Stability and folding of the ferredoxin from
the hyperthermophilic archaeon Acidianus ambivalens, J Inorg Biochem 78, 35-41.
Gomes, C. M., Vicente, J. B., Wasserfallen, A., and Teixeira, M. (2000) Spectroscopic studies and
characterization of a novel electron-transfer chain from Escherichia coli involving a flavorubredoxin and its
flavoprotein reductase partner, Biochemistry 39, 16230-16237.
Frazao, C., Silva, G., Gomes, C. M., Matias, P., Coelho, R., Sieker, L., Macedo, S., Liu, M. Y., Oliveira, S.,
Teixeira, M., Xavier, A. V., Rodrigues-Pousada, C., Carrondo, M. A., and Le Gall, J. (2000) Structure of a
dioxygen reduction enzyme from Desulfovibrio gigas, Nat Struct Biol 7, 1041-1045.
Silva, G., Oliveira, S., Gomes, C. M., Pacheco, I., Liu, M. Y., Xavier, A. V., Teixeira, M., Legall, J., and
Rodrigues-pousada, C. (1999) Desulfovibrio gigas neelaredoxin. A novel superoxide dismutase integrated in
a putative oxygen sensory operon of an anaerobe, Eur J Biochem 259, 235-243.
Romao, C. V., Liu, M. Y., Le Gall, J., Gomes, C. M., Braga, V., Pacheco, I., Xavier, A. V., and Teixeira, M.
(1999) The superoxide dismutase activity of desulfoferrodoxin from Desulfovibrio desulfuricans ATCC
27774, Eur J Biochem 261, 438-443.
Gomes, C. M., Lemos, R. S., Teixeira, M., Kletzin, A., Huber, H., Stetter, K. O., Schafer, G., and Anemuller, S.
(1999) The unusual iron sulfur composition of the Acidianus ambivalens succinate dehydrogenase complex,
Biochim Biophys Acta 1411, 134-141.
Das, T. K., Gomes, C. M., Teixeira, M., and Rousseau, D. L. (1999) Redox-linked transient deprotonation at
the binuclear site in the aa(3)-type quinol oxidase from Acidianus ambivalens: implications for proton
translocation, Proc Natl Acad Sci U S A 96, 9591-9596.
Aagaard, A., Gilderson, G., Gomes, C. M., Teixeira, M., and Brzezinski, P. (1999) Dynamics of the binuclear
center of the quinol oxidase from Acidianus ambivalens, Biochemistry 38, 10032-10041.
LeGall, J., Liu, M. Y., Gomes, C. M., Braga, V., Pacheco, I., Regalla, M., Xavier, A. V., and Teixeira, M. (1998)
Characterisation of a new rubredoxin isolated from Desulfovibrio desulfuricans 27774: definition of a new
family of rubredoxins, FEBS Lett 429, 295-298.
Gomes, C. M., and Teixeira, M. (1998) Ambineela, an unusual blue protein isolated from the archaeon
Acidianus ambivalens, Biochem Biophys Res Commun 249, 23-25.
Gomes, C. M., and Teixeira, M. (1998) The NADH oxidase from the thermoacidophilic archaea Acidianus
ambivalens: isolation and physicochemical characterisation, Biochem Biophys Res Commun 243, 412-415.
Gomes, C. M., Huber, H., Stetter, K. O., and Teixeira, M. (1998) Evidence for a novel type of iron cluster in
the respiratory chain of the archaeon Sulfolobus metallicus, FEBS Lett 432, 99-102.
Gomes, C. M., Faria, A., Carita, J. C., Mendes, J., Regalla, M., Chicau, P., Huber, H., Stetter, K. O., and
Teixeira, M. (1998) Di-cluster, seven-iron ferredoxins from hyperthermophilic Sulfolobales, J Biol Inorg
Chem 3, 499-507.
Gomes, C. M., Silva, G., Oliveira, S., LeGall, J., Liu, M. Y., Xavier, A. V., Rodrigues-Pousada, C., and Teixeira,
M. (1997) Studies on the redox centers of the terminal oxidase from Desulfovibrio gigas and evidence for
its interaction with rubredoxin, J Biol Chem 272, 22502-22508.
Giuffre, A., Gomes, C. M., Antonini, G., D'Itri, E., Teixeira, M., and Brunori, M. (1997) Functional properties
of the quinol oxidase from Acidianus ambivalens and the possible catalytic role of its electron donor-studies on the membrane-integrated and purified enzyme, Eur J Biochem 250, 383-388.
Teixeira, M., Batista, R., Campos, A. P., Gomes, C., Mendes, J., Pacheco, I., Anemuller, S., and Hagen, W. R.
(1995) A seven-iron ferredoxin from the thermoacidophilic archaeon Desulfurolobus ambivalens, Eur J
Biochem 227, 322-327.
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CV Cláudio M. Gomes – Sept 2015
Chapters in books
1.
2.
3.
4.
5.
Carvalho, S. B., Cardoso, I., Botelho, H. M., Yanamandra, K., Fritz, G., Gomes, C. M., and Morozova-Roche, L. A.
(2014) Chapter 18 - Structural Heterogeneity and Bioimaging of S100 Amyloid Assemblies, in Bio-nanoimaging
(Uversky, V. N., and Lyubchenko, Y. L., Eds.), pp 197-212, Academic Press, Boston.
Henriques, B. J., Rodrigues, J. V., and Gomes, C. M. (2012) Riboflavin and beta-oxidation Flavoenzymes, in B
Vitamins and Folate: Chemistry, Analysis, Function and Effects (Preedy, V. R., Ed.), pp 611-632, The Royal
Society of Chemistry.
Botelho, H. M., Fritz, G., and Gomes, C. M. (2012) Analysis of S100 Oligomers and Amyloids, in Amyloid
Proteins: Methods and Protocols (Sigurdsson, E. M., Calero, M., and Gasset, M., Eds.), Springer
Science+Business Media, New York.
Leal, S. S., and Gomes, C. M. (2010) Iron-Sulfur Clusters, Protein Folds, and Ferredoxin Stability, in Protein
Folding and Metal Ions (Gomes, C. M., and Wittung-Stafshede, P., Eds.), pp 81-96, CRC Press.
Gomes, C. M., and Wittung-Stafshede, P. (2010) Metal Ions, Protein Folding, and Conformational States, in
Protein Folding and Metal Ions (Gomes, C. M., and Wittung-Stafshede, P., Eds.), pp 3-11, CRC Press.
Edited book
Protein Folding and Metal Ions: Mechanisms, Biology and Disease
by Claudio M. Gomes (Editor), Pernilla Wittung-Stafshede (Editor)
CRC Press. 2010. ISBN-13: 978-1439809648 ISBN-10: 143980964X
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