58º Congresso Brasileiro de Genética
Resumos do 58o Congresso Brasileiro de Genética • 11 a 14 de setembro de 2012
Rafain Palace Hotel e Convention Center • Foz do Iguaçu • PR • Brasil
www.sbg.org.br - ISBN 978-85-89109-06-2
169
The first cytogenetic study in Vampyrum
spectrum (Phyllostomidae, Chiroptera,
Mammalia) in Brazil
Sousa, RF¹; Tenório, RCCO²; Venere, PC²; Faria, KC¹
1
Departamento de Ciências Biológicas, UNEMAT - Campus de Nova Xavantina; 2Departamento de Ciências Biológicas e
da Saúde, UFMT - Campus Universitário do Araguaia.
[email protected]
Keywords: Cytogenetics, Phyllostomidae, bat.
The chromosomes analysis is an effective method that contributes to the knowledge of the structure and molecular
organization and behavior of chromosomes. Knowing more about the cytogenetic aspects from species of Brazilian
bats may contribute to understand the systematics of Neotropical bats as a whole. The Vampyrum spectrum specie was
cytogenetically described in the 70s in a specimen captured in Central America, nevertheless, until now there was
only the diploid number identification for this specie. The present study has aimed to accomplish the first cytogenetic
study for a Brazilian sample of Vampyrum spectrum. Conventional staining techniques were carried out with Giemsa,
banding C, impregnation by silver nitrate (AgNO3) and the CMA3 technique. The specie presented a diploid number
equal to 30 (2n=30) and the number of autosomal arms was equal to 56 (NA=56). The autosomal set consists of: 11
pairs of chromosomes meta-submetacentrics ranging from large to small size (1 to 7 and 10 to 13) and three pairs of
chromosomes subtelocentric small size medium (8, 9 and 14). The X chromosome is medium submetacentric and the
Y chromosome a small acrocentric. The C banding technique revealed regions of constitutive heterochromatin in the
pericentromeric regions of all chromosomes. The nucleoli organizer region was evidenced in the secondary constriction
of the long arm of chromosome 1. With the CMA3 technique it was possible to observe transverse bands in the
chromosome enabling the individualization of chromosome pairs. This is the first study conducted cytogenetically
with V. spectrum in Brazil, and it was possible to identify specific chromosomal regions not yet known for the specie.
Financial support: CAPES, FAPEMAT and UNEMAT.