Free Radical Biology and Medicine ∎ (∎∎∎∎) ∎∎∎–∎∎∎
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Free Radical Biology and Medicine
journal homepage: www.elsevier.com/locate/freeradbiomed
Corrigendum
Corrigendum to “Involvement of oxidative stress
in 4-vinylcyclohexene-induced toxicity in Drosophila melanogaster”
[Free Radic. Biol. Med. 71 (2014) 99–108]
Amos Olalekan Abolaji a,b,n, Jean Paul Kamdem b,c, Thiago Henrique Lugokenski b,d, Thallita
Kalar Nascimento b, Emily Pansera Waczuk b, Ebenezer Olatunde Farombi a,
Élgion Lúcio da Silva Loreto e, João Batista Teixeira Rocha b,nn
a
Drug Metabolism and Molecular Toxicology Research Laboratories, Department of Biochemistry, Faculty of Basic Medical Sciences, College of Medicine,
University of Ibadan, Ibadan, Nigeria
b
Departamento de Bioquimica e Biologia Molecular, Bioquímica Toxicológica, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria,
Santa Maria, Rio Grande do Sul 97105-900, Brazil
c
Departamento de Bioquímica, Instituto de Ciências Básica da Saúde, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS CEP 90035-003, Brazil
d
Universidade Federal do Pampa - UNIPAMPA - Campus Caçapava do Sul - RS - Brazil
e
Laboratório de Biologia Molecular – LabDros, Universidade Federal de Santa Maria, Santa Maria, Brazil, Rio Grande do Sul 97105-900, Brazil
Volume 71 (2014), in the article (http://dx.doi.org/10.1016/j.free
radbiomed.2014.03.014) “Involvement of oxidative stress in 4-vinylcyclohexene-induced toxicity in Drosophila melanogaster” by Amos
Olalekan Abolaji, Jean Paul Kamdem, Thiago Henrique Lugokenski,
Thallita Kalar Nascimento, Emily Pansera Waczuk, Ebenezer Olatunde
Farombi, Élgion Lúcio da Silva Loreto, and João Batista Teixeira Rocha,
pages 99–108: the Biocuration Manager of the Comparative Toxicogenomics Database (http://ctdbase.org/) noted that the sequence stated
as Nrf-2 in our paper is in fact that of the Nrf-2 inhibitor (Keap1). After
investigation, we noted that “inhibitor” was inadvertently omitted
from the vial label. Possibly the person who requested or synthesized it
forgot to include the word inhibitor in the request process or in the
labeling stage. Thus, we regret the mistake, but the sequence remains
the same. Thus, we would like to replace “Nrf-2” in all the places that it
appears in the paper, as well as in Fig. 4C and Table 1, with “Nrf-2
inhibitor (Keap1)” for the convenience of the readers. The use of “Nrf-2
inhibitor” instead of “‘Keap1” is not generally used, but in this context
it is used to explain how this error occurred. The error in the indicated
DOI of original article: http://dx.doi.org/10.1016/j.freeradbiomed.2014.03.014
Corresponding author at: Universidade Federal de Santa Maria, Departamento
de Bioquimica e Biologia Molecular, Bioquímica Toxicológica, Centro de Ciências
Naturais e Exatas, Campus Universitário, Av roraima 1000, 97105-900 Santa Maria,
Rio Grande do Sul, Brazil. Fax: þ 55 55 8104 0207.
nn
Corresponding author. Fax: þ 55 55 8104 0207.
E-mail addresses: [email protected], [email protected] (A.O. Abolaji),
[email protected], [email protected] (J.B.T. Rocha).
n
http://dx.doi.org/10.1016/j.freeradbiomed.2014.11.008
0891-5849/& 2014 Elsevier Inc. All rights reserved.
Please cite this article as: Abolaji, AO; et al. Corrigendum to “Involvement of oxidative stress in 4-vinylcyclohexene-induced toxicity in
Drosophila melanogaster” [Free Radic. Biol. Med. 71.... Free Radic. Biol. Med. (2014), http://dx.doi.org/10.1016/j.freeradbiomed.2014.11.008i
2
A.O. Abolaji et al. / Free Radical Biology and Medicine ∎ (∎∎∎∎) ∎∎∎–∎∎∎
Table 1
Sequences of qPCR primers.
Fig. 4. (C) Induction of Nrf-2 inhibitor (Keap1) gene expression level after 5 days
of 4-vinylcyclohexene exposure in D. melanogaster. Relative mRNA levels of Nrf-2
inhibitor (Keap1) after exposure of D. melanogaster to 10 mM, 100 mM, and 1 mM
4-vinylcyclohexene are shown. Data are presented as the means 7 SEM of three
independent biological replicates carried out in quadruplicate. *P o 0.05 vs
control.
name did not affect the conclusions of the paper. The corrected Table 1
and Fig. 4C with legend are shown below.
The increased mRNA gene expression of the Nrf-2 inhibitor
(Keap1) may represent an adaptive response to an increase in
Keap1 oxidation by electrophile species formed from VCH metabolism in the flies. This information should replace the discussion
on the increased mRNA gene expression of Nrf-2 in the paper.
Primer
Sequence
HSP27 left
HSP27 right
HSP83 left
HSP83 right
HSP70 left
HSP70 right
SOD left
SOD right
Catalase left
Catalase right
Nrf-2 inhibitor (Keap1) left
Nrf-2 inhibitor (Keap1) right
MPK2 left
MAPK2 right
GPDH left
GPDH right
Tubulin left
Tubulin right
50 -AAAGATGGCTTCCAGGTGTG-30
50 -CCCTTGGGCAGGGTATACTT-30
50 -CAAATCCCTGACCAACGACT-30
50 -CGCACGTACAGCTTGATGTT-30
50 -CGGAGTCTCCATTCSGGTGT-30
50 -GCTGACGTTCAGGATTCCAT-30
50 -GGAGTCGGTGATGTTGACCT-30
50 -GTTCGGTGACAACACCAATG-30
50 -ACCAGGGCATCAAGAATCTG-30
50 -AACTTCTTGGCCTGCTCGTA-30
50 -CCAACTTCCTCAAGGAGCAG-30
50 -CGGCGACAAATATCATCCTT-30
50 -GGCCACATAGCCTGTCATCT-30
50 -ACCAGATACTCCGTGGCTTG-30
50 -ATGGAGATGATTCGCTTCGT-30
50 -GCTCCTCAATGGTTTTTCCA-30
50 -ACCAATGCAAGAAAGCCTTG-30
50 -ATCCCCAACAACGTGAAGAC-30
We apologize to the community for this inadvertent omission in
the paper, and we thank the Biocuration Manager of the Comparative
Toxicogenomics Database for alerting us about this error.
Please cite this article as: Abolaji, AO; et al. Corrigendum to “Involvement of oxidative stress in 4-vinylcyclohexene-induced toxicity in
Drosophila melanogaster” [Free Radic. Biol. Med. 71.... Free Radic. Biol. Med. (2014), http://dx.doi.org/10.1016/j.freeradbiomed.2014.11.008i