www.biochemj.org
Biochem. J. (2012) 444, 405–415 (Printed in Great Britain)
doi:10.1042/BJ20112219
SUPPLEMENTARY ONLINE DATA
The disordered N-terminal region of dengue virus capsid protein contains
a lipid-droplet-binding motif
*Instituto de Bioquı́mica Médica, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, 21941-902, Brazil, †Instituto de Medicina Molecular, Faculdade de Medicina da
Universidade de Lisboa, Lisbon, 1649-028, Portugal, ‡Centro Nacional de Ressonância Magnética Nuclear, Universidade Federal do Rio de Janeiro and National Institute of Structural
Biology and Bioimage, Rio de Janeiro, RJ, 21941-902, Brazil, §Polo Avançado de Xerém, Universidade Federal do Rio de Janeiro, Duque de Caxias, RJ, 25245-390, Brazil, Instituto
Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, RJ, 21040-360, Brazil, and ¶Laboratório de Genomica Estrutural, Instituto de Biofı́sica Carlos Chagas Filho, Universidade
Federal do Rio de Janeiro, Rio de Janeiro, RJ, 21941-902, Brazil
Figure S1 (13 C,1 H)-HSQC spectra of DENV C protein in the presence (red)
and absence (black) of LDs, showing that the overall protein structure is
maintained
Figure S2 (13 C,1 H)-HSQC spectra of pep5–26 in the presence (red) and
absence (black) of LDs
(a) Cα region. (b) Cβ region. (c) Methyl region of the spectra. Hα/Cα paired resonances in
three different regions of the (13 C,1 H)-HSQC spectra do not show significant chemical shift
changes, reinforcing the previous conclusion of the absence of pep5–26–LD binding.
1
To whom correspondence should be addressed (email [email protected]).
c The Authors Journal compilation c 2012 Biochemical Society
Biochemical Journal
Ivo C. MARTINS*†, Francisco GOMES-NETO*‡, André F. FAUSTINO†, Filomena A. CARVALHO†, Fabiana A. CARNEIRO§,
Patricia T. BOZZA, Ronaldo MOHANA-BORGES¶, Miguel A. R. B. CASTANHO†, Fábio C. L. ALMEIDA*‡, Nuno C. SANTOS† and
Andrea T. DA POIAN*1
I. C. Martins and others
Table S1
Virus strains and sequences used to construct the alignment with different Flavivirus spp. capsid proteins in Figure 3(a) in the main paper
N.A., not applicable (query sequence).
Virus name
Abbreviation
Strain
NCBI accession number
Total score
Query coverage
Taxonomy (Group, species )
Dengue virus serotype 2
West Nile virus
WNV serotype Kunjin
Japanese encephalitis virus
St. Louis encephalitis virus
Murray Valley encephalitis virus
Kokobera virus
Kedougou virus
Zika virus
Spondweni virus
Aroa virus
Iguape virus
Usutu virus
Alfuy virus
Bagaza virus
Ilheus virus
Rocio virus
DENV-2
WNV
WNV-K
JEV
SLEV
MVEV
KOKV
KEDV
ZIKV
SPOV
AROAV
IGUV
USUV
ALFV
BAGV
ILHV
ROCV
PR-159S1
K6453
MRM62C
YL
GML 903797
MVE-1-51
AusMRM 32
DakAR D1470
MR766
SM-6 V1
BEAN 4073
SPAn 71686
SAAR-1776
MRM3929
DakAR B209
Original
SPM 34675
1R6R_A
ADM88862.1
AAP78941.1
AAL93186.1
ABN11822.1
NP_051124.1
YP_001040007
ABI54477.1
ABI54475.1
ABI54480.1
YP_001040004
AAV34154.1
AAS59401.1
AAX82481.1
YP_002790883
YP_001040006
AAV34158.1
N.A.
76.6
75.5
75.5
79.3
73.2
85.9
85.5
84.0
87.8
83.6
80.1
79.7
77.0
76.6
65.9
64.7
N.A.
91
91
93
93
83
91
96
98
99
95
87
91
93
85
94
86
Dengue virus group, Dengue virus
Japanese enc. virus group, West Nile virus
Japanese enc. virus group, West Nile virus
Japanese enc. virus group, Japanese enc. virus
Japanese enc. virus group, St. Louis enc. virus
Japanese enc. virus group, Murray Valley enc. virus
Kokobera virus group, Kokobera virus
Dengue virus group, Kedougou virus
Spondweni virus group, Zika virus
Spondweni virus group, Zika virus
Aroa virus group, Aroa virus
Aroa virus group, Aroa virus
Japanese enc. virus group, Usutu virus
Japanese enc. virus group, Murray Valley enc. virus
Ntaya virus group, Bagaza virus
Ntaya virus group, Ilheus virus
Ntaya virus group, Ilheus virus
Received 22 December 2011/6 March 2012; accepted 19 March 2012
Published as BJ Immediate Publication 19 March 2012, doi:10.1042/BJ20112219
c The Authors Journal compilation c 2012 Biochemical Society