1552
EBUS APELLA: A NEW MODEL FOR THE STUDY OF PARKINSON´S DISEASE HISTOPATHOLOGICAL CHANGES INDUCED
IN SUBSTANCE NIGRA BY MPTP IN C
K.S. Silva1,2, E.C. Franco3, W.L.A. Pereira4, L.E.C. da Silva5, L.B. Torres6, B.H.S. Araujo6, F.R. Cabral7
1
Department of Breeding and Production of Lab Animals, Evandro Chagas Institute, Ananindeua, 2INCTC, Ribeirão Preto, 3Evandro
Chagas Institute, Ananindeua, 4ISPA, Universidade de Federal Rural da Amazônia, 5Graduation Student, Universidade Federal do
Pará, Belém, 6Laboratório de Neurologia Experimental, Universidade Federal de São Paulo, 7InCe, Instituto Israelita de Ensino e
Pesquisa Albert Einstein (InCe/Einstein), São Paulo, Brazil
Objectives: Evaluate the possible use of Cebus apella as an new experimental model of PD.
Methods: The subject comprised one capuchin monkey, male, 9 years. The animal was injected with 0.3 mg/kg (IM) of MPTP (10
injections) over 30 days, with 3 days of interval between injections. Over these 30 days, the symptoms and behaviors observed
were: stereotyped movements, tongue ptosis, stiff tail, sudden drop and worst performance in behavioral test, after MPTP injection.
Following the protocol period, the subject was sedated in the home cage with intramuscular injections of ketamine (2.5 mg/kg) and
xylazine (0.125 mg/kg). After the induction of deep anesthesia, the animal was perfused and, the skull and dura mater were
removed. The right brain hemisphere was cut on a cryostat at 20µm thick. Slices were stained with Haematoxin.eosin., reticulin,
Nissl. All procedures were in accordance with an approved Institutional Animal Care and Use Committee protocol of the Brazilian
Institute of the Environment.
Results and conclusion: Most of the neurons of the substantia nigra exhibited a central chromatolysis and a process of
degeneration of axons. It was also observed small aggregates of glial cells which indicates reactive gliosis in this mid brain region.
In summary our histopathological analysis indicates a degeneration of the dopaminergic neurons of the substantia nigra, similar
from that observed in human PD pacients.