Additional file 2: Individuals samples in this study. Columns indicate voucher or tissue number, municipality and state of the sampled locality, locality code (L), sequenced mtDNA fragments, mtDNA haplotype (Hap.), mtDNA main clade or subclade (clade), allele number for alleles 1 and 2 of sequenced nuclear fragments, and individual number in the STRUCTURE analysis (STR). mtDNA number CFBH-T10831 CFBH-T10832 CFBH-T10833 CFBH-T10834 CFBH-T10841 PHV1450 PHV1451 MTJ26 MTJ27 MTJ53 MTJ54 MTJ55 MTJ56 MTJ71 MTJ72 MTJ84 MTJ85 CFBH-T3642 CFBH-T3645 CFBH-T3650 CFBH-T3658 CFBH-T3663 CFBH-T9161 MNRJ35341 MNRJ35342 CFBH-T9233 CFBH-T4151 CFBH-T9129 CFBH-T9131 CFBH-T9132 CFBH-T9140 CFBH-T9141 CFBH-T9147 CFBH-T9176 CFBH-T9181 CFBH-T3572 CFBH-T3577 CFBH-T3578 CFBH-T3586 CFBH-T3591 CFBH-T3596 CFBH-T3605 CFBH-T3609 MRT 5806 MRT 5975 MTR 13599 MTR 13608 municipality Guaramiranga Guaramiranga Guaramiranga Guaramiranga Guaramiranga São Desidério São Desidério Januária Januária Januária Januária Januária Januária Januária Januária Januária Januária Areia Recife Recife Recife Recife Itacaré Itacaré Itacaré Aurelino Leal Uruçuca Uruçuca Uruçuca Uruçuca Uruçuca Uruçuca Uruçuca Uruçuca Uruçuca Itabuna Itabuna Itabuna Itabuna Itabuna Itabuna Itabuna Itabuna Jussari Jussari Camacan Camacan state CE CE CE CE CE BA BA MG MG MG MG MG MG MG MG MG MG PB PE PE PE PE BA BA BA BA BA BA BA BA BA BA BA BA BA BA BA BA BA BA BA BA BA BA BA BA BA L 1 1 1 1 1 2 2 3 3 3 3 3 3 3 3 3 3 4 5 5 5 5 6 6 6 7 8 8 8 8 8 8 8 8 8 9 9 9 9 9 9 9 9 10 10 11 11 fragments control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 ND1,ND2 ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 ND1,ND2 control,ND1,ND2 ND1,ND2 control,ND1,ND2 control,ND1,ND2 ND1 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 -1- Hap. hap1 hap1 hap2 hap1 hap1 hap289 hap290 hap285 hap286 hap285 hap287 hap286 hap285 hap285 hap288 hap287 hap285 hap222 hap223 hap223 hap223 hap223 hap248 hap273 hap274 hap280 hap232 hap244 hap245 hap246 hap247 hap248 hap249 hap245 hap249 hap214 hap215 hap216 hap217 hap218 hap219 hap220 hap221 hap268 hap267 hap260 hap261 clade G G G G G P P P P P P P P P P P P N N N N N N N N N N N N N N N N N N N N N N N N N N N N N N a.polypep. al.1 al.2 nuclear crystallin al.1 al.2 rhodopsin al.1 al.2 STR 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 1 3 1 1 48 9 1 50 48 48 48 48 37 5 37 35 5 42 37 35 42 5 1 36 35 36 2 3 2 2 49 9 9 48 48 48 49 48 36 37 35 35 36 35 39 36 37 36 35 39 36 36 1 1 1 1 150 150 150 150 150 150 150 150 150 150 150 106 108 110 1 112 129 141 117 118 126 128 - 1 1 1 1 150 150 150 150 150 150 150 150 150 150 150 107 109 111 1 113 129 142 146 119 105 5 - 1 1 2 2 20 2 20 2 20 2 2 2 7 20 20 8 11 2 2 2 11 11 11 11 11 2 2 2 11 1 11 11 11 11 11 2 2 2 2 20 24 11 24 11 24 24 24 7 20 20 8 11 2 2 2 11 11 11 11 21 2 22 11 11 22 11 11 11 11 11 36 35 5 38 38 29 9 36 36 36 35 36 39 9 105 139 134 114 105 105 135 84 11 2 11 11 2 11 11 11 11 11 11 11 11 11 11 11 mtDNA number MTR 13609 MTR 13610 MTR 13620 MTR 13621 MTR16007 MTR16011 MTR16045 CFBH-T12560 CFBH-T12561 CFBH-T12564 CFBH-T12565 PUCMG2 MNRJ38920 MNRJ38921 CFBH-T2389 PUCMG1 MTR 13384 MTR 13385 MTR 13472 MTR 13482 MTR 13594 MTR 13595 MTR 13646 CFBH-T4062 CFBH-T4063 CFBH-T4064 CFBH-T4065 CFBH-T4066 CFBH-T4067 CFBH-T4068 CFBH-T4069 CFBH-T4070 CFBH-T4071 CFBH-T7689 AF402 CFBH-T7652 CFBH-T7660 CFBH-T7664 JC 794 CFBH-T7482 CFBH-T7492 CFBH-T7532 MNRJ38327 MNRJ38329 CFBH-T10842 MTR 11543 MTR 11548 MTR15751 MTR15756 MTR 11588 MRT 1252 MRT 1253 MTR 12585 MTR 12609 municipality Camacan Camacan Camacan Camacan Camacan Camacan Camacan Camacan Camacan Camacan Camacan Sta. Maria do Salto Porto Seguro Porto Seguro Grão Mogol Cristália Trancoso Trancoso Trancoso Trancoso Trancoso Trancoso Trancoso Caraíva Caraíva Caraíva Caraíva Caraíva Caraíva Caraíva Caraíva Caraíva Caraíva Prado Santa Bárbara Nova Lima Nova Lima Nova Lima Mariana Ouro Branco Ouro Branco Ouro Branco Catas Altas Catas Altas Belo Horizonte Santa Marta Santa Marta Espera Feliz Espera Feliz Córrego Calçado Ibitirama Ibitirama Ibitirama Ibitirama state BA BA BA BA BA BA BA BA BA BA BA MG BA BA MG MG BA BA BA BA BA BA BA BA BA BA BA BA BA BA BA BA BA BA MG MG MG MG MG MG MG MG MG MG MG MG MG ES ES ES ES ES ES ES L 11 11 11 11 11 11 11 11 11 11 11 12 13 13 14 15 16 16 16 16 16 16 16 17 17 17 17 17 17 17 17 17 17 18 19 20 20 20 21 22 22 22 23 23 24 25 25 26 26 27 28 28 28 28 fragments control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 -2- Hap. hap262 hap261 hap263 hap264 hap277 hap278 hap260 hap281 hap282 hap283 hap284 hap258 hap275 hap265 hap207 hap279 hap255 hap256 hap257 hap258 hap259 hap230 hap265 hap224 hap225 hap226 hap227 hap228 hap229 hap224 hap227 hap230 hap231 hap239 hap266 hap238 hap94 hap237 hap153 hap92 hap93 hap94 hap190 hap191 hap116 hap157 hap158 hap193 hap276 hap159 hap141 hap142 hap146 hap141 clade a.polypep. al.1 al.2 N N N N N N N N N N N N N N N N N N N N N N N N N N N N N N N N N N N N C N C C C C C C C C C C N C C C C C 5 9 5 35 38 38 5 5 36 5 5 38 46 1 1 38 5 5 29 5 29 38 5 5 35 5 5 38 38 5 20 1 5 8 20 5 29 31 8 5 1 1 24 - 9 39 29 9 9 35 1 35 36 39 38 36 5 1 47 38 43 38 44 44 35 38 39 38 9 8 1 38 5 9 9 27 5 9 8 8 30 32 9 5 1 24 25 - nuclear crystallin al.1 al.2 136 144 105 114 148 117 143 131 99 81 131 132 66 114 116 99 114 66 138 125 126 80 65 67 68 87 88 83 84 90 84 77 78 - 137 119 145 147 121 149 105 117 81 140 117 133 99 115 115 82 117 127 121 125 126 81 66 66 69 87 89 83 85 91 84 77 79 - rhodopsin al.1 al.2 STR 2 11 11 11 11 11 11 2 11 11 2 11 11 11 2 11 2 11 11 11 11 11 11 11 11 11 2 11 2 11 2 11 2 11 11 2 2 7 10 11 2 2 11 2 2 11 2 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 39 38 40 41 42 - 11 11 11 11 11 11 11 11 11 11 11 11 11 11 11 11 11 11 11 11 11 11 11 8 11 11 11 11 11 11 11 11 11 11 11 2 11 8 10 11 11 11 11 2 11 11 11 mtDNA number MTR 10732 MTR 10733 MTR15775 CFBH-T9177 CFBH-T5935 CFBH-T5936 CFBH-T5945 CFBH-T10466 CFBH-T9043 CFBH-T9046 MTR 12132 MTR 12200 MTR 12248 CFBH-T3362 CFBH-T3367 CFBH-T3370 CFBH-T3373 CFBH-T9008 CFBH-T9009 CFBH-T9014 CFBH-T9080 CFBH-T9178 CFBH-T5953 MNRJ34956 CFBH-T3393 CFBH-T3397 CFBH-T3400 CFBH-T3407 LSH1 LSH38 LSH39 LSH60 MTR 1237 CFBH-T9324 CFBH-T9325 Tfreitas CFBH-T4380 CFBH-T9331 CFBH-T10355 CFBH-T3377 CFBH-T3381 CFBH-T3385 CFBH-T3387 CFBH-T3422 CFBH-T2317 CFBH-T5086 CFBH-T5087 CFBH-T3099 CFBH-T7699 CFBH-T7705 CFBH-T7712 CFBH-T7717 CFBH-T7727 CFBH-T0867 municipality Caparaó Caparaó Dores do Rio Preto Sooretama Sooretama Sooretama Sooretama Linhares Linhares Linhares Linhares Linhares Linhares Aracruz Aracruz Aracruz Aracruz Santa Leopoldina Santa Leopoldina Santa Leopoldina Santa Leopoldina Santa Tereza Santa Tereza Santa Tereza Vila Velha Vila Velha Vila Velha Vila Velha Guarapari Guarapari Guarapari Anchieta S. José do Calçado Teresópolis Teresópolis Itaipava Petrópolis Guapimirim Seropédica Itaguaí Itaguaí Itaguaí Itaguaí Itaguaí Cristina Cristina Cristina S. R. Passa Quatro Bauru Bauru Bauru Bauru Bauru Sto. Ant. do Pinhal state MG MG ES ES ES ES ES ES ES ES ES ES ES ES ES ES ES ES ES ES ES ES ES ES ES ES ES ES ES ES ES ES ES RJ RJ RJ RJ RJ RJ RJ RJ RJ RJ RJ MG MG MG SP SP SP SP SP SP SP L 29 29 30 31 31 31 31 32 32 32 32 32 32 33 33 33 33 34 34 34 34 35 35 35 36 36 36 36 37 37 37 38 39 40 40 41 42 43 44 45 45 45 45 45 46 46 46 47 48 48 48 48 48 49 fragments control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control control,ND1,ND2 ND1 control,ND1,ND2 control,ND1,ND2 ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 -3- Hap. hap269 hap156 hap194 hap291 hap233 hap234 hap235 hap250 hap242 hap243 hap270 hap271 hap272 hap208 hap41 hap209 hap210 hap240 hap240 hap241 hap110 hap206 hap236 hap236 hap206 hap211 hap212 hap213 hap251 hap252 hap253 hap254 hap140 hap204 hap204 hap203 hap68 hap205 hap112 hap44 hap208 hap42 hap43 hap44 hap13 hap72 hap73 hap21 hap95 hap96 hap95 hap95 hap97 hap5 clade N C C N N N N N N N N N N N C N N N N N C N N N N N N N N N N N C C C C C C C C N C C C C C C C C C C C C C a.polypep. al.1 al.2 nuclear crystallin al.1 al.2 rhodopsin al.1 al.2 STR 44 43 45 47 46 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 27 26 8 27 82 82 82 67 23 11 23 11 5 1 5 9 5 5 5 5 5 5 1 1 24 5 40 5 5 24 24 35 1 1 9 1 5 1 5 9 1 14 9 9 1 41 1 35 45 9 97 120 122 114 129 140 99 43 82 128 99 102 98 121 123 124 129 131 105 43 101 5 100 103 11 1 1 11 11 11 11 11 11 11 11 11 11 11 19 11 2 2 1 2 2 11 11 2 2 11 11 11 11 11 11 11 8 11 11 11 11 11 11 11 2 2 11 11 24 24 104 104 2 11 5 5 1 5 130 95 100 96 2 2 5 1 1 1 1 1 1 6 5 5 5 5 5 5 5 5 6 1 1 1 34 6 6 6 6 5 5 5 5 5 5 10 5 6 75 45 54 4 44 45 6 56 58 30 13 11 70 6 76 45 55 94 45 46 20 57 13 59 13 20 11 6 2 2 2 2 11 2 11 2 2 2 2 2 2 2 1 10 2 2 20 20 11 13 13 11 2 2 2 2 2 2 12 2 12 2 mtDNA number CFBH-T0868 CFBH-T1278 CFBH-T6526 CFBH-T3170 CFBH-T3174 CFBH-T0336 CFBH-T2588 CFBH-T3628 CFBH-T3633 CFBH-T3638 CFBH-T3659 CFBH-T3803 CFBH-T3806 CFBH-T3834 CFBH-T3836 CFBH-T8867 CFBH-T8890 CFBH-T8891 CFBH-T8900 CFBH-T2664 CFBH-T4008 CFBH-T4009 CFBH-T4010 CFBH-T6033 CFBH-T6048 CFBH-T6053 CFBH-T6054 H-466 H-478 H-485 H-488 UF94/18 UF94/30 CFBH-T1956 CFBH-T2516 CFBH-T2517 CFBH-T3491 CFBH-T3896 CFBH-T3897 CFBH-T3910 CFBH-T5522 AF1430 AF1435 AF1443 CFBH-T3953 CFBH-T3954 MTR 16675 MTR10400 MTR10402 IT-H0023 IT-H0025 CFBH-T10514 CFBH-T3856 CFBH-T3912 municipality Sto. Ant. do Pinhal S. Luís Paraitinga S. Luís Paraitinga Nat. da Serra Nat. da Serra Ubatuba Ubatuba Ubatuba Ubatuba Ubatuba Ubatuba Caraguatatuba Caraguatatuba Caraguatatuba Caraguatatuba São Sebastião São Sebastião São Sebastião São Sebastião Ilha Bela Ilha Bela Ilha Bela Ilha Bela Ilha Bela Ilha Bela Ilha Bela Ilha Bela Bertioga Bertioga Bertioga Bertioga Bertioga Bertioga Cubatão Cubatão Cubatão Cubatão Cubatão Cubatão Cubatão Cubatão Santos Santos Santos Santos Santos Santo André Santo André Santo André São Bernardo São Bernardo Itanhaém Itanhaém Itanhaém state SP SP SP SP SP SP SP SP SP SP SP SP SP SP SP SP SP SP SP SP SP SP SP SP SP SP SP SP SP SP SP SP SP SP SP SP SP SP SP SP SP SP SP SP SP SP SP SP SP SP SP SP SP SP L 49 50 50 51 51 52 52 52 52 52 52 53 53 53 53 54 54 54 54 55 55 55 55 55 55 55 55 56 56 56 56 56 56 57 57 57 57 57 57 57 57 58 58 58 58 58 59 59 59 60 60 61 61 61 fragments control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 ND1,ND2 ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 -4- Hap. hap5 hap8 hap86 hap22 hap4 hap4 hap16 hap47 hap48 hap49 hap50 hap53 hap13 hap54 hap55 hap107 hap108 hap109 hap109 hap17 hap63 hap64 hap65 hap17 hap63 hap85 hap13 hap185 hap183 hap109 hap184 hap109 hap183 hap10 hap14 hap15 hap45 hap57 hap58 hap59 hap75 hap179 hap180 hap181 hap61 hap62 hap13 hap154 hap155 hap163 hap164 hap113 hap56 hap60 clade a.polypep. al.1 al.2 C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C 5 5 5 5 5 5 14 6 6 6 5 5 5 5 5 5 5 5 5 5 5 5 5 5 6 5 6 5 5 5 6 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 10 5 5 5 5 6 5 5 5 5 6 15 6 6 6 16 6 5 6 17 6 5 6 6 5 6 5 5 6 6 5 6 17 5 5 6 12 10 5 5 5 5 17 1 1 17 5 18 5 5 10 5 10 10 5 5 10 nuclear crystallin al.1 al.2 7 16 22 6 32 4 24 4 45 49 49 22 41 13 22 6 22 22 22 22 22 5 6 22 6 21 23 47 23 63 22 6 22 41 46 35 22 22 11 52 20 7 17 6 4 25 5 25 5 22 49 49 22 9 28 41 22 22 22 22 22 22 9 22 22 19 22 22 9 41 37 22 22 41 22 22 22 22 22 12 53 35 rhodopsin al.1 al.2 STR 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 1 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 10 2 2 2 5 2 2 2 2 12 2 2 2 2 2 2 2 2 2 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 93 94 91 92 95 96 97 100 98 99 2 2 2 2 2 2 11 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 19 2 2 2 2 2 2 2 2 2 mtDNA number CFBH-T5869 CFBH-T5870 CFBH-T5871 AF122 AF147 AF151 AF160 AF169 CFBH-T2246 CFBH-T2248 CFBH-T4031 CFBH-T4032 CFBH-T5144 CFBH-T6957 CFBH-T6976 CFBH-T7034 CFBH-T6952 CFBH-T7322 CFBH-T10697 CFBH-T8433 CFBH-T8434 CFBH-T8547 CFBH-T8548 CFBH-T8683 CFBH-T5155 AF403 MTR 9957 MTR 9958 MTR 9959 MTR 9960 MTR 9963 CFBH-T3108 CFBH-T8274 CFBH-T8275 CFBH-T8276 Uniban2555 CFBH-T3180 CFBH-T3184 CFBH-T3189 CFBH-T3190 CFBH-T3194 CFBH-T3195 CFBH-T3202 CFBH-T3204 CFBH-T3206 CFBH-T4426 CFBH-T4427 MTR15559 IT-H0437 IT-H0438 IT-H0439 IT-H0459 IT-H0461 IT-H0462 municipality Peruíbe Peruíbe Peruíbe Iguape Iguape Iguape Iguape Iguape Iguape Iguape Iguape Iguape Iguape Iguape Iguape Iguape Pariquera Açú Ilha Comprida Cananéia Cananéia Cananéia Cananéia Cananéia Cananéia Ilha do Cardoso São Paulo São Paulo São Paulo São Paulo São Paulo São Paulo Cotia Biritiba Mirim Biritiba Mirim Biritiba Mirim Biritiba Mirim Santa Isabel Santa Isabel Santa Isabel Santa Isabel Santa Isabel Santa Isabel Santa Isabel Santa Isabel Santa Isabel Santa Isabel Santa Isabel Iperó Juquitiba Juquitiba Juquitiba Juquitiba Juquitiba Juquitiba state SP SP SP SP SP SP SP SP SP SP SP SP SP SP SP SP SP SP SP SP SP SP SP SP SP SP SP SP SP SP SP SP SP SP SP SP SP SP SP SP SP SP SP SP SP SP SP SP SP SP SP SP SP SP L 62 62 62 63 63 63 63 63 63 63 63 63 63 63 63 63 64 65 66 66 66 66 66 66 67 68 68 68 68 68 68 69 70 70 70 70 71 71 71 71 71 71 71 71 71 71 71 72 73 73 73 73 73 73 fragments control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 -5- Hap. hap83 hap83 hap84 hap143 hap144 hap145 hap147 hap90 hap11 hap12 hap66 hap67 hap74 hap88 hap89 hap90 hap87 hap91 hap103 hap103 hap104 hap105 hap104 hap106 hap32 hap149 hap113 hap188 hap189 hap5 hap182 hap13 hap29 hap101 hap102 hap5 hap5 hap23 hap24 hap24 hap25 hap26 hap27 hap28 hap29 hap69 hap70 hap192 hap167 hap168 hap45 hap169 hap66 hap113 clade a.polypep. al.1 al.2 C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C 5 4 4 5 5 5 5 5 5 5 5 1 19 5 5 10 5 5 5 5 5 5 5 5 5 5 5 5 4 5 5 5 17 5 28 5 5 5 5 5 5 12 6 5 5 5 5 5 5 5 17 5 5 5 16 5 5 16 5 5 5 5 1 16 19 5 5 16 5 5 5 5 23 5 5 5 10 5 5 5 5 5 5 5 17 5 17 6 5 5 5 11 5 11 6 5 5 5 5 10 10 5 10 5 nuclear crystallin al.1 al.2 26 28 11 9 9 8 20 60 9 13 13 13 13 62 20 41 41 6 28 6 14 6 33 22 6 20 4 34 35 36 6 35 41 22 20 35 29 8 11 9 11 20 11 61 27 13 13 11 13 22 22 22 41 11 32 9 26 6 22 4 6 6 22 22 22 37 6 9 11 11 11 35 rhodopsin al.1 al.2 2 2 2 2 2 2 9 2 2 2 2 2 2 2 2 2 2 1 2 2 2 2 2 2 2 2 2 2 2 2 2 2 12 2 2 1 14 12 12 2 2 1 2 2 2 2 2 2 9 2 2 2 2 2 2 11 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 11 2 2 6 2 2 2 2 12 6 18 2 2 STR 101 102 106 107 103 104 105 108 111 109 110 112 113 115 116 114 117 118 119 120 124 121 122 123 125 127 mtDNA number IT-H0463 IT-H0468 IT-H0538 AF303 AF304 AF316 AF423 ALC86/77 ALC86/80 ALC86/81 ALC87/1 ALC87/3 ALC87/5 H023 H035 IT-H0207 IT-H0208 IT-H0209 UF88/60 H0300 H0355 H0356 H0366 IT-H0573 IT-H0580 IT-H0581 IT-H0583 IT-H0610 IT-H0612 IT-H0613 CFBH-T5679 CFBH-T5680 CFBH-T5681 CFBH-T5682 CFBH-T5683 CFBH-T5684 CFBH-T5685 CFBH-T5686 CFBH-T5687 CFBH-T5688 CFBH-T5041 CFBH-T1034 CFBH-T1035 IIH-149 IIH-172 IIH-224 II - H046 II - H114 CFBH-T11188 CFBH-T9476 CFBH-T7881 CFBH-T7897 CFBH-T7898 CFBH-T9512 municipality Juquitiba Juquitiba Juquitiba Carapicuíba Carapicuíba Carapicuíba Carapicuíba Piedade Piedade Piedade Piedade Piedade Piedade Piedade Piedade Piedade Piedade Piedade Piedade Buri Buri Buri Buri Buri Buri Buri Buri Buri Buri Buri Ribeirão Grande Ribeirão Grande Ribeirão Grande Ribeirão Grande Ribeirão Grande Ribeirão Grande Ribeirão Grande Ribeirão Grande Ribeirão Grande Ribeirão Grande Guapiara Ribeirão Branco Ribeirão Branco Wenceslau Brás Wenceslau Brás Wenceslau Brás Pinhalão Ortigueira Ortigueira Teodoro Sampaio Teodoro Sampaio Teodoro Sampaio Teodoro Sampaio Teodoro Sampaio state SP SP SP SP SP SP SP SP SP SP SP SP SP SP SP SP SP SP SP SP SP SP SP SP SP SP SP SP SP SP SP SP SP SP SP SP SP SP SP SP SP SP SP PR PR PR PR PR PR SP SP SP SP SP L 73 73 73 74 74 74 74 75 75 75 75 75 75 75 75 75 75 75 75 76 76 76 76 76 76 76 76 76 76 76 77 77 77 77 77 77 77 77 77 77 78 79 79 80 80 80 81 82 82 83 83 83 83 83 fragments control,ND1,ND2 control,ND1,ND2 ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND2 control,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 -6- Hap. hap45 hap170 hap171 hap45 hap5 hap148 hap150 hap5 hap113 hap113 hap160 hap161 hap162 hap113 hap186 hap165 hap166 hap66 hap113 hap174 hap151 hap187 hap152 hap173 hap80 hap174 hap175 hap151 hap152 hap151 hap76 hap77 hap78 hap79 hap80 hap81 hap82 hap79 hap80 hap80 hap71 hap6 hap7 hap176 hap177 hap178 hap172 hap151 hap121 hap199 hap200 hap201 hap201 hap202 clade a.polypep. al.1 al.2 C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 17 5 5 5 5 5 5 5 5 10 5 5 5 5 5 5 5 5 5 5 5 5 5 5 4 5 5 5 5 5 5 5 5 5 5 5 1 5 5 5 5 5 5 17 5 17 5 5 5 5 5 17 5 5 10 5 5 5 5 5 5 10 5 10 10 5 5 5 5 10 5 5 10 5 10 5 5 10 5 5 1 5 5 1 5 1 1 1 5 1 1 nuclear crystallin al.1 al.2 6 9 9 20 34 28 41 28 64 41 6 20 64 20 10 20 29 10 9 26 11 64 28 26 29 40 10 9 10 10 10 10 86 9 10 93 10 10 41 11 11 20 41 28 41 28 20 22 41 11 20 29 11 11 29 26 11 27 29 9 11 26 9 11 10 10 26 26 10 26 13 9 26 26 26 26 rhodopsin al.1 al.2 STR 12 2 6 1 2 12 10 2 12 12 2 12 10 1 1 10 1 10 10 1 12 1 10 1 12 10 1 1 1 2 1 1 1 1 12 10 10 10 12 12 10 10 10 10 128 126 129 130 131 132 135 133 134 141 140 139 137 138 136 142 143 144 145 146 147 148 149 150 151 152 153 154 155 156 157 12 2 6 12 2 12 2 2 6 12 2 6 1 1 1 1 12 10 1 1 12 1 1 12 12 12 12 12 12 12 2 12 12 2 12 12 12 12 12 12 12 12 12 10 mtDNA number CFBH-T7899 CFBH-T9513 CFBH-T9514 IIBPH1333 IIBPH1173 IIBPH1191 IIBPH1336 IIBPH1342 IIBPH1353 IIBPH1356 MVS243 MVS244 MVS242 CFBH-T3619 CFBH-T9193 CFBH-T12598 CFBH-T3084 CFBH-T3085 CFBH-T3086 CFBH-T3304 CFBH-T3306 CFBH-T3310 CFBH-T3312 K970 CFBH-T10261 CFBH-T3665 CFBH-T3666 CFBH-T3672 CFBH-T3679 CFBH-T3314 CFBH-T9201 CFBH-T9202 CFBH-T9202 CFBH-T9204 CFBH-T9205 CFBH-T9206 CFBH-T9207 CFBH-T9208 CFBH-T9209 CFBH-T9210 CFBH-T9211 CFBH-T3316 CFBH-T3320 CFBH-T3323 CFBH-T3343 CFBH-T3348 CFBH-T3351 CFBH-T9194 CFBH-T9195 CFBH-T9196 CFBH-T9197 CFBH-T9198 CFBH-T9199 CFBH-T9200 municipality Teodoro Sampaio Teodoro Sampaio Teodoro Sampaio Canindeyú Itapúa Itapúa Itapúa Itapúa Itapúa Itapúa Adrianópolis Adrianópolis Ribeira Quatro Barras Quatro Barras Antonina Antonina Antonina Antonina Morretes Morretes Morretes Morretes São José dos Pinhais Guaratuba Guaratuba Guaratuba Guaratuba Guaratuba Itapoá Massaranduba Massaranduba Massaranduba Massaranduba Massaranduba Massaranduba Massaranduba Massaranduba Massaranduba Massaranduba Massaranduba Rio dos Cedros Rio dos Cedros Rio dos Cedros Blumenau Blumenau Blumenau Blumenau Blumenau Blumenau Blumenau Blumenau Blumenau Blumenau state SP SP SP PAR PAR PAR PAR PAR PAR PAR PR PR SP PR PR PR PR PR PR PR PR PR PR PR PR PR PR PR PR SC SC SC SC SC SC SC SC SC SC SC SC SC SC SC SC SC SC SC SC SC SC SC SC SC L 83 83 83 84 85 85 85 85 85 85 86 86 87 88 88 89 89 89 89 90 90 90 90 91 92 92 92 92 92 93 94 94 94 94 94 94 94 94 94 94 94 95 95 95 96 96 96 96 96 96 96 96 96 96 fragments control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 ND1 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 -7- Hap. hap202 hap200 hap200 hap119 hap117 hap118 hap120 hap121 hap113 hap113 hap196 hap197 hap195 hap46 hap124 hap3 hap18 hap19 hap20 hap30 hap31 hap32 hap33 hap123 hap111 hap51 hap51 hap52 hap33 hap34 hap131 hap132 hap133 hap134 hap135 hap136 hap134 hap136 hap137 hap138 hap139 hap35 hap36 hap37 hap38 hap39 hap40 hap125 hap126 hap126 hap127 hap128 hap129 hap130 clade C C C C C C C C C C c1 C C c1 c1 c1 C c1 C c1 c1 C c1 c1 c1 c1 c1 c1 c1 c1 c1 c1 c1 c1 c1 c1 c1 c1 c1 c1 c1 c1 c1 c1 c1 c1 c1 c1 c1 c1 c1 c1 c1 c1 a.polypep. al.1 al.2 nuclear crystallin al.1 al.2 rhodopsin al.1 al.2 STR 158 159 160 161 162 163 164 165 166 167 168 169 170 171 172 173 174 175 176 177 178 179 180 181 182 - 5 5 5 5 5 5 5 5 5 4 33 4 4 4 5 4 5 4 5 1 5 5 1 5 5 1 5 5 5 33 5 4 5 5 7 7 5 1 10 10 9 9 9 9 20 9 28 92 28 48 41 26 28 29 11 11 27 26 9 9 9 9 9 9 9 13 26 13 11 41 27 26 27 38 9 11 10 10 10 10 12 10 10 12 10 10 10 5 6 1 10 1 2 1 10 12 12 12 12 12 12 12 12 10 12 6 17 2 1 12 2 12 5 4 4 4 5 5 5 4 4 7 1 4 7 1 4 7 7 4 4 13 4 4 4 4 7 1 4 1 7 5 5 7 5 7 1 5 7 7 4 7 7 1 7 7 4 4 7 7 1 7 1 7 7 7 8 8 8 50 22 27 9 9 74 73 38 9 26 15 39 40 42 14 11 15 15 72 15 73 15 9 26 9 11 11 51 11 26 11 9 9 9 41 11 73 9 11 41 11 9 15 9 15 11 9 9 73 72 41 10 1 1 1 5 10 5 12 5 5 1 1 1 1 6 1 6 10 1 - 2 2 5 1 6 12 5 6 5 5 1 5 12 5 6 1 6 5 1 - mtDNA number CFBH-T10731 CFBH-T10732 CFBH-T1735 PUCRS CFBH-T8092 CFBH-T8093 CFBH-T8094 CFBH-T8095 CFBH-T8096 CFBH-T8097 CFBH-T8098 CFBH-T8099 ELMG453 CFBH-T11195 CFBH-T11196 MVS398 CFBH-T10793 CFBH-T10794 CFBH-T9184 CFBH-T9186 CFBH-T9188 EMLG234 EMLG235 EMLG236 EMLG237 EMLG253 EMLG453 flona062007 flona1603 MNRJ33006 MNRJ33012 CFBH-T8298 UFRGST667 CFBH-T9213 CFBH-T1338 CFBH-T3051 CFBH-T3354 CFBH-T3361 CFBH-T5092 CFBH-T5731 CFBH-T8885 CFBH-T8933 municipality Gov. Celso Ramos Gov. Celso Ramos Itapema S.. Am. Imperatriz Anitápolis Anitápolis Anitápolis Anitápolis Anitápolis Anitápolis Anitápolis Anitápolis Ponte Serrada Ponte Serrada Fernandes Pinheiro Salete Xavantina Xavantina Xavantina Xavantina Xavantina Guatambú Guatambú Guatambú Guatambú Guatambú Guatambú Guatambú Guatambú Mato Castelhano Mato Castelhano Cotiporã Nova Roma do Sul Bento Gonçalves outgroups R. arenarum R. icterica R. icterica R. granulosa R. merianae R.merianae R. icterica R. icterica state SC SC SC SC SC SC SC SC SC SC SC SC SC SC PR SC SC SC SC SC SC SC SC SC SC SC SC SC SC RS RS RS RS RS L 97 97 98 99 100 100 100 100 100 100 100 100 101 101 102 103 104 104 104 104 104 105 105 105 105 105 105 105 105 106 106 107 108 109 fragments control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1,ND2 control,ND1 control,ND1 control,ND1,ND2 control,ND1,ND2 control,ND1 ND1 -8- Hap. hap114 hap115 hap9 hap198 hap98 hap295 hap99 hap100 hap100 hap100 hap296 hap297 hap299 hap122 hap292 hap304 hap293 hap294 hap299 hap299 hap299 hap299 hap299 hap300 hap299 hap299 hap292 hap292 hap302 hap303 hap298 hap305 hap301 clade c1 c1 c1 c1 c1 S c1 c1 c1 c1 S S S C S S S S S S S S S S S S S S S S S S S a.polypep. al.1 al.2 nuclear crystallin al.1 al.2 rhodopsin al.1 al.2 STR 183 184 185 193 186 187 189 190 188 191 192 194 195 196 4 1 4 4 21 1 22 1 1 - 1 1 4 1 22 1 22 1 1 - 11 11 18 26 154 11 155 71 155 14 15 11 11 155 11 155 11 155 5 1 12 3 3 25 6 3 6 2 6 15 25 16 16 3 5 21 51 53 53 57 21 - 5 22 52 54 56 56 22 - 28 159 151 152 156 156 155 156 157 158 28 160 151 153 156 156 156 155 5 5 12 3 10 1 2 3 3 3 - 12 3 5 2 2 15 4 25 - 58 21 21 13 61 55 21 59 22 21 22 22 55 60 156 159 156 162 163 163 155 156 160 156 163 163 163 161 25 3 3 3 3 25 25 25 3 25 25 3 25 25 25 25 25 15