World Practical Course II International School of Biochemistry, Molecular & Cell Biology Current tools in cell biology: probing normal and pathological cell functions IV International Symposium on Myosin V - 21 Years of History Current tools in cell biology: probing normal and pathological cell functions st 1 Week Aug 02 – Arrival O7:00 – 08:00 08:00 – 09:00 09:00 – 10:00 10:00 – 10:30 10:30 – 11:30 11:30 – 12:30 12:30 – 13:30 13:30 – 18:00 18:00 – 19:00 19:00 – 20:00 20:30 – 24:00 Aug 03 Aug 04 Lecture 1 Lecture 2 Lecture 5 Lecture 6 Lecture 3 Lecture 4 Lecture 7 Lecture 8 Aug 05 Aug 06 Lecture 9 Lecture 10 Lecture 13 Lecture 14 Lecture 11 Lecture 12 Lecture 15 Lecture 16 Hotel - UFRJ Coffee Break Lunch Practical 1-8 UniGen – Departamento de Genética e Biologia Molecular Practical 1-8 Practical 1-8 UFRJ – Hotel Dinner Late activity Practical 1-8 World Practical Course II International School of Biochemistry, Molecular & Cell Biology Current tools in cell biology: probing normal and pathological cell functions IV International Symposium on Myosin V - 21 Years of History Lectures (Quinhentão/CCS/UFRJ) Regulation of Actin Assembly and Cell Motility 1. by IQGAPs 2. Principles of FRET microscopy 3. Cell motility in Cancer 4. Unconventional myosins: are we biased by names? 5. RNAs and Proteins Traffic in Myelinated Axons: Inside the Cell and from Outside the Cell 6. Regulation of the actomyosin cytoskeleton by Rho GTPases during cancer cell invasion 7. Actin cytoskeleton modulates calcium signalling during maturation and fertilization 8. General Methods for Studying Transcription and Nuclear Processes 9. 10. 11. 12. 13. 14. Calcium Signaling : a Meeting point for cellular and structural biology 15. Two-hybrid technologies in proteomics research 16. Microtubules, Rho GTPases and cell polarity George Bloom University of Virginia Horst Wallrabe Enilza Espreafico University of Virginia University of São Paulo John Mercer McLaughlin Research Institute Jose Sotelo Ins. Invest. Biol. Clemente Estable Suranganie Dharmawardhane University of Puerto Rico Luigia Santella Stazione Zoologica Anton Dohrn Primal de Lanerolle University of Illinois Roy Larson Charlie Boone Margaret Titus Universidade de São Paulo Niversity of Toronto University of Minnesota Jose Rodriguez-Medina Universidad of Puerto Rico Ernesto Carafoli University of Padova Igor Stagljar University of Toronto Gregg Gundersen Columbia University Lab Classes Title 1. 2. 3. 4. 5. 6. 7. 8. Rac activity in cytoskeletal organization iRNA approaches in cancer biology Actin IQGAP1 association in cells Cell-to-cell RNA transfer in sciatic nerve 2+ T. cruzi and cell invasion: the role of Ca and Myosin V Cell mRNA localization New tools in cellular bioinformatics Quantitative FRET microscopy UniGen – Departamento de Genética e Biologia Molecular Faculty Suranganie Dharmawardhane Enilza Espreafico George Bloom John Mercer/Jose Sotelo Verônica Salerno/ Bill Provance Jeffrey E. Gerst Margaret Titus Horst Wallrabe TA Lab World Practical Course II International School of Biochemistry, Molecular & Cell Biology Current tools in cell biology: probing normal and pathological cell functions IV International Symposium on Myosin V - 21 Years of History IV International Symposium on Myosin V - 21 Years of History Friday, 07 Talk Evidence for an involvement in myosin Va in cell spreading and motility 08:30 - 09:00 Enilza Espreafico Antagonistic influences of Tctex-1 and Lfc during axon formation 09:00 - 09:30 Alfredo Cáceres Signaling from Amyloid to Tau in Alzheimer's Disease 09:30 - 10:00 George Bloom 10:00 - 10:30 Break Calcium dysregulation and mitochondrial stress in Huntington's striatal neurons 10:30 - 11:00 Ernesto Carafoli Calcium and fertilization: an old phenomenon in the new light? 11:00 - 11:30 Luigia Santella 11:30 - 12:00 Miguel Seabra Friday, 07 16:00 - 16:30 16:30 - 17:00 17:00 - 17:30 17:30 - 18:00 18:00 - 18:30 18:30 - 19:00 19:00 - 19:30 Poster Section 19:30 - 21:00 Talk Expression of cytoskeletal proteins of the protozoan Giardia intestinalis after its in vitro treatment with experimental chemicals. Javier Ambrosio Gustavo Brum Running to save neurons L. C. Cameron Break Gonzalo Ferreira Roy Larson Carlos Más UniGen – Departamento de Genética e Biologia Molecular World Practical Course II International School of Biochemistry, Molecular & Cell Biology Current tools in cell biology: probing normal and pathological cell functions IV International Symposium on Myosin V - 21 Years of History IV International Symposium on Myosin V - 21 Years of History Saturday, 08 Talk mRNA trafficking – A whole genome in transit? Jeffrey E. Gerst Molecular Motors in the Nucleus Primal de Lanerolle Nuclear-cytoskeleton interaction sin cell polarity, migration and disease Gregg Gundersen Break Trafficking in polarized epithelial cells (Transferrin receptor) Horst Wallrabe 08:30 - 09:00 09:00 - 09:30 09:30 - 10:00 10:00 - 10:30 10:30 - 11:00 11:00 - 11:30 11:30 - 12:00 Alexandre Romeiro Saturday, 08 16:00 - 16:30 16:30 - 17:00 17:00 - 17:30 17:30 - 18:00 18:00 - 18:30 18:30 - 19:00 19:00 - 19:30 Talk Cancer Genetics in Colombia Ignacio Briceño Regulation of the Rho GTPase Rac in metastatic breast cancer by dietary compounds and small molecule inhibitors Suranganie Dharmawardhane William Provance Break Verônica Salerno Schwan cell (peripheral glia) supply peripheral axons with ribosomes and RNAs José Sotelo Martha Sorenson Poster Section 19:30 - 21:00 UniGen – Departamento de Genética e Biologia Molecular World Practical Course II International School of Biochemistry, Molecular & Cell Biology Current tools in cell biology: probing normal and pathological cell functions IV International Symposium on Myosin V - 21 Years of History IV International Symposium on Myosin V - 21 Years of History Sunday, 09 Talk Identification and Characterization of novel cancer associated genes Josane de Freitas Sousa João Pedro Werneck de Castro Charlie Boone Break Myosin V proteins interact with multiple Rab GTPases James Goldenring The glycogen granule and its proteins: their regulatory roles Terry Graham Learning, memory, and immune system: How does immunity control the brain function? Jonathan Kipnis 08:30 - 09:00 09:00 - 09:30 09:30 - 10:00 10:00 - 10:30 10:30 - 11:00 11:00 - 11:30 11:30 - 12:00 Sunday, 09 16:00 - 16:30 16:30 - 17:00 17:00 - 17:30 17:30 - 18:00 18:00 - 18:30 18:30 - 19:00 19:00 - 19:30 Poster Section 19:30 - 21:00 Talk Role of reactive oxygen species in contraction-mediated glucose transport in skeletal muscle Abram Katz The EEG in the cerebral metabolism Daniel Botero Myosin-Va and cell-to-cell RNA transfer John Mercer Break José Rodriguez-Medina Driving Biological Discoveries Using the Membrane Yeast Two-Hybrid (MYTH) Technology: lessons from the human Epidermal Growth Factor Receptor (EGFR) Igor Stagljar Margaret Titus UniGen – Departamento de Genética e Biologia Molecular World Practical Course II International School of Biochemistry, Molecular & Cell Biology Current tools in cell biology: probing normal and pathological cell functions IV International Symposium on Myosin V - 21 Years of History Current tools in cell biology: probing normal and pathological cell functions nd 2 O7:00 – 08:00 08:00 – 09:00 09:00 – 10:00 10:00 – 10:30 10:30 – 11:30 11:30 – 12:30 12:30 – 13:30 13:30 – 18:00 18:00 – 19:00 19:00 – 20:00 20:30 – 24:00 Week Aug 11 Aug 12 Lecture 17 Lecture 18 Lecture 21 Lecture 22 Lecture 19 Lecture 20 Lecture 23 Lecture 24 Aug 13 Aug 14 Lecture 25 Lecture 26 Lecture 29 Lecture 30 Lecture 27 Lecture 28 Lecture 31 Lecture 32 Hotel - UFRJ Coffee Break Lunch Practical 1-8 Practical 1-8 Practical 1-8 UFRJ – Hotel Dinner Late activity Practical 1-8 Aug 15 – Departure UniGen – Departamento de Genética e Biologia Molecular World Practical Course II International School of Biochemistry, Molecular & Cell Biology Current tools in cell biology: probing normal and pathological cell functions IV International Symposium on Myosin V - 21 Years of History Lectures (Library Auditorium/CCS/UFRJ) 17. Dissecting protein:protein interactions: The modern toolbox. 18. The glycogen granule and its protiens: their regulatory roles 19. Adult neurogenesis in vitro and in vivo 20. 21. 22. Neuronal polarity: Cellular and molecular 23. mechanism 24. 25. Glucose transport into skeletal muscle 26. Methods of investigation in energy metabolism 27. Cancer Genetics 28. Moving messages – How to localize mRNAs in vivo Strategies for studying cytoskeletal proteins of 29. parasites as drug targets. James Goldenring Vanderbilt University Terry Graham University of Guelph Jonathan Kipnis Miguel Seabra Gonzalo Ferreira Gustavo Brum University of Virginia Imperial College Universidad de la República Universidad de la República IInv. Med. Mercedes y Martín Ferreyra Olympus Inc. Karolinska Institute Universidad de La Sabana Universidad de La Sabana Weizmann Institute of Science Alfredo Cáceres Carlos Más Abram Katz Daniel Botero Ignacio Briceño Jeffrey E. Gerst Javier Ambrosio 30. L. C. Cameron 31. Martha Sorenson Universidad Nacional Autonoma de Mexico Fed. U. of State of Rio de Janeiro Federal University of Rio de Janeiro 32. Lab Classes 9. 10. 11. 12. 13. 14. 15. 16. Title Sponsors real time PCR Immunochemical recognition of cytoskeletal proteins Measurement of glucose transport in isolated muscle Relation beetwen EEG and Neurometabolism Spinning disk confocal microscopy Stem cells and Heart Damage Immunohistochemical techniques to determine neural precursor cells and "baby" neurons in adult rodent brain 2+ Ca signalling in muscle cells UniGen – Departamento de Genética e Biologia Molecular Faculty Ignacio Briceño Javier Ambrosio Abram Katz Daniel Botero Alfredo Cáceres JP Werneck de Castro Jonathan Kipnis Martha Sorenson TA Lab World Practical Course II International School of Biochemistry, Molecular & Cell Biology Current tools in cell biology: probing normal and pathological cell functions IV International Symposium on Myosin V - 21 Years of History 17. Confocal Microscopy UniGen – Departamento de Genética e Biologia Molecular Carlos Más