RE-EPITHELIZATION EVALUATION AND MITOTIC ACTIVITY OF CRUDE
EXTRACT FROM Caesalpinia peltophoroides BENTH
BUENO, F.B.1, MACHARETH, M.A.D.1, LEITE-MELLO, E.V.S.2, MELLO, J.C.P.1
1. Departamento de Farmácia, Universidade Estadual de Maringá. Maringá, Paraná, Brasil.
2. Departamento de Ciências Morfológicas, Universidade Estadual de Maringá. Maringá, Paraná, Brasil.
[email protected]
Keywords: Caesalpinia peltophoroides, Polyphenols, Wound healing
1. Introduction
The difficulty in wound healing may be a
consequence
of pathological conditions
associated with diabetes, autoimmune disorders,
ischemia and venous stasis. Research have been
carried out by topical application of drugs that
accelerate the wound healing process. This
research aimed to evaluate the process of reepithelialization and mitotic activity during
wound healing treated with crude extract (CE)
from Caesalpinia peltophoroides bark.
Table 1. Average values of the re-epithelialized
area.
x=mean, VC= variation coefficient, *P<0,05
2. Methods
Two wounds were made on the backs of the
animals. One was treated with base gel and the
other with gel containing CE for 4, 7, 10 and 14
days. With the wounds fragments histological
analysis of the re-epithelialized area, in length
(4 and 7 days) and thickness (10 and 14 days),
were carried out. In basal and suprabasal layer
the number of blocked metaphases with
vincristine sulfate 0,5 mg/kg were also
analysed.
3. Results
The measurements of length at 4 days and
thickness at 10 and 14 days of treatment with
gel containing CE are greater than those treated
with base gel (P <0.05) (Table 1). The number
of metaphases with the gel containing CE and
the base gel is increased until 7 and 10 days,
respectively (Figure 1). These data show that
while the wound treated with gel containing CE
has an accentuated mitotic process at 7 days, the
base gel provides this result at 10 days.
Figure 1. Number of cells in metaphase.
*P<0,05
4. Conclusion
The test carried out showed that CE of C.
peltophoroides has action on the tissue repair,
offering conditions that promote faster healing.
The observed conditions were the formation of a
larger re-epithelialized area at 4 days of
treatment and thicker in the end of the healing
period analyzed, and a greater number of
metaphases in the first 7 days of treatment.
Acknowledgments
CNPq and CAPES/Proap for the financial
support of this work.