59º Congresso Brasileiro de Genética
Resumos do 59o Congresso Brasileiro de Genética • 16 a 19 de setembro de 2013
Hotel Monte Real Resort • Águas de Lindóia • SP • Brasil
www.sbg.org.br - ISBN 978-85-89109-06-2
STUDY OF ACE AND ACTN3 POLYMORPHISMS
AND PHYSICAL PERFORMANCE IN
BRAZILIAN ARMY VOLUNTEERS
Santos, CGM1, Gonçalves, MM2; Pompeu, FAMS3; Moura-Neto, RS4; Silva, R5.
Instituto de Biologia do Exército (IBEX), Exercito Brasileiro, Rio de Janeiro, RJ; 2 Instituto de Pesquisa da Capacitação
Física do exército (IPCFEx), Exército Brasileiro, Rio de Janeiro, RJ; 3 Escola de Educação Física e Desportos, UFRJ, Rio
de Janeiro, RJ; 4 Instituto de Biologia, UFRJ, Rio de janeiro, RJ; 5 Instituto de Biofísica Carlos Chagas Filho, UFRJ, Rio de
janeiro, RJ
1
[email protected]
Keywords: SNP, performance, trainability, genomic
Military troops need systematic training and athletic skills to reach a satisfactory physical performance and operational
success. Athletic performance is a multifactorial complex human trait, but as environmental factors has been
insufficient to explain some prominent phenotypes, there is a possible genetic predisposition. Thus, two SNPs have
been widely studied in sports performance. One gene of Angiotensin Converting Enzyme (ACE) and another to
alpha-actinina3 (ACTN3). The I allele (Alu indel, intron 16 - ACE) was associated with a higher performance for
endurance exercises while the D allele was associated to Sprint/power activities. On the other hand, the ACTN3
configures a common nonsense polymorphism where homozygous carriers of allele R, were allegedly related to a
higher performance in Sprint/power activities. Few data are available on specialized troops. Thus, a preliminary study
has been performed. The selected population was composed of 33 individuals of the Brazilian Army, with around to
13 years of career, male (31.12±1.62 years), body fat (23.61±6.77)%; weight (84.37±9.59)kg, height (1.75±0.07)
m. Anthropometric measurements were taken of the volunteers. Standardized Physical training (c20-20 - Brazilian
Army) was submitted to them for 7 months. Some Peripheral blood was collected before and after training. DNA
was extracted from blood collected in EDTA using comercial MAGNEX® kit. Three physical diagnostic evaluations
tests were performed (cooper and maximum repetitions of arm flexion, Abdominal flexion above the horizontal bar)
with 1, 4 and 7 months after the initial assessment. Analysis of variance was performed with SPSS® for the analysis
of genotypes. Comparing the group that had at least one I allele (I/I or I/D - 2763.64 m) and individuals with
DD (2890m) to the distance traveled in 12 minutes, there was no statistically significant difference (p = 0.271),
as well as to gain race with training (distance traveled in the seventh month less distance at baseline - I/I or I/D)
(181.82 m) and DD (170.00 m) with p = 0.863. No difference was also found comparing the results of cooper test
and separately the genotypes DD, ID or II. For ACTN3 genotypes there was no statistically significant difference
between the mean of distance traveled X/X (2662.50 m), R/X (2834.62 m), R/R (2870.00 m), or post training gain
running X/X (100.00 m), R/X (192.31 m), R/R (220.00 m), (p = 0.315 and 0.350). There was also no statistically
significant differences for ACTN3 and ACE among groups regarding the number the bar flexion, arm flexions or
Abdominal flexions performed. The results do not suggest an association of several ACE and ACTN3 genotypes in
the population studied. However, more studies are needed to define the influence of SNPs in physical performance.
Financial Support: Brazillian Army and Faperj - BrazilHuman oncoprotein c-Yes: cDNA amplification and cloning
Viviane A. Pereira, Flavio S. Silva, Caroline C. Elias. Mariana M. Alves and Regina Affonso
Centro de Biotecnologia, IPEN/CNEN, S. P., Brazil
Keywords: c-Yes; hRPL10, oncoprotein, recombinant, kinase
191
Cytoplasmic tyrosine kinases of the Src family (SFKs) play important roles in signal transduction induced by a large
number of extracellular stimuli including growth factors. This family is represented by nine members, including c-Src,